CN113440570A

CN113440570A - Traditional Chinese medicine composition for reducing blood sugar and losing weight and preparation thereof

Info

Publication number: CN113440570A
Application number: CN202110696399.1A
Authority: CN
Inventors: 刘向前; 郑贤珠
Original assignee: Changsha Broad Ocean Bio Science And Technique Co ltd
Current assignee: Changsha Broad Ocean Bio Science And Technique Co ltd
Priority date: 2021-06-23
Filing date: 2021-06-23
Publication date: 2021-09-28

Abstract

The invention discloses a traditional Chinese medicine composition for reducing blood sugar and losing weight and a preparation thereof, belonging to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 23-27 parts of Chinese yam, 8-12 parts of black beans, 8-12 parts of phaseolus calcaratus, 9-11 parts of balsam pear, 13-16 parts of trichosanthes root, 14-16 parts of jerusalem artichoke and 13-16 parts of radix acanthopanacis senticosi. The flash extraction method is used for carrying out flash extraction on the traditional Chinese medicine composition, and realizes the high-efficiency extraction of effective components by effectively regulating and controlling the process steps and parameters in the extraction process. The traditional Chinese medicine composition is a safe and effective traditional Chinese medicine hypoglycemic drug, and has the effect of losing weight on the basis of effectively controlling the content of blood sugar.

Description

Traditional Chinese medicine composition for reducing blood sugar and losing weight and preparation thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine composition for reducing blood sugar and losing weight and a preparation thereof.

Background

At present, with the acceleration of life rhythm and environmental pollution, the incidence rate of hyperglycemia diseases shows an upward trend.

Short-time and disposable hyperglycemia has no serious damage to human bodies, such as transient hyperglycemia in a stress state or in emotional agitation and high stress; a large amount of sugar is taken at a time, and transient hyperglycemia can also occur; subsequently, the blood glucose level gradually returns to normal. However, long-term hyperglycemia not only causes organ hypertrophy and obesity, but also causes pancreatic damage and insulin resistance. Hyperglycemia causes pathological changes of all tissues and organs of the whole body, and leads to the occurrence of acute and chronic complications, such as dehydration, electrolyte disorder, nutrient deficiency, resistance reduction, renal dysfunction, neuropathy, fundus oculi pathological changes, cardiovascular and cerebrovascular diseases, diabetic foot and the like.

The currently clinically used blood sugar lowering drugs mainly include biguanides (representing drugs such as metformin), sulfonylureas (representing drugs such as glipizide and gliclazide), alpha-glycosidase inhibitors (representing drugs such as acarbose), and insulin sensitizers (representing drugs such as rosiglitazone and pioglitazone) in addition to insulin. However, western medicines usually have limitations and adverse reactions, for example, biguanide hypoglycemic medicines have great harm to intestines and stomach, sulfonylurea medicines may cause leucopenia, alpha-glycosidase inhibitors may cause gastrointestinal tract reactions, insulin sensitizers may cause water retention, and the like, so that the research and development of traditional Chinese medicine hypoglycemic medicines with hypoglycemic effects, safety and no side effects is a difficult problem to be overcome urgently in the medical science and technology field and pharmaceutical industry at home and abroad at present.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for reducing blood sugar and losing weight and a preparation thereof, which are used for solving the problems in the prior art, so that the traditional Chinese medicine composition has a remarkable blood sugar reducing effect and can inhibit obesity caused by high-fat diet.

In order to achieve the purpose, the invention provides the following scheme:

one of the technical schemes of the invention is as follows: the traditional Chinese medicine composition for reducing blood sugar and losing weight is provided, and the raw materials comprise the following components in parts by weight:

23-27 parts of Chinese yam, 8-12 parts of black beans, 8-12 parts of phaseolus calcaratus, 9-11 parts of balsam pear, 13-16 parts of trichosanthes root, 14-16 parts of jerusalem artichoke and 13-16 parts of radix acanthopanacis senticosi.

Further, the raw materials comprise the following components in parts by weight:

25 parts of Chinese yam, 10 parts of black bean, 10 parts of phaseolus calcaratus, 10 parts of balsam pear, 15 parts of trichosanthes root, 15 parts of jerusalem artichoke and 15 parts of acanthopanax root.

The second technical scheme of the invention is as follows: provides a Chinese medicinal preparation for reducing blood sugar and losing weight, which comprises the extract of the Chinese medicinal composition.

Further, the extraction method of the traditional Chinese medicine composition extract comprises the following steps:

(1) pretreatment of raw materials: drying the raw materials at 45-50 ℃;

(2) flash extraction: mixing the pretreated raw materials with an extraction solvent for flash extraction, and filtering to obtain a filtrate after extraction is finished;

(3) centrifuging the filtrate: centrifuging the obtained filtrate at 10-12 deg.C and 3000r/min for 5-6min, and collecting supernatant;

(4) concentration: concentrating the supernatant to obtain extract;

(5) and (3) purification: and (4) adding water into the extract obtained in the step (4) for suspension, extracting by using an organic solvent, removing an organic layer, concentrating a water phase, and drying in vacuum to obtain the traditional Chinese medicine composition extract.

Further, the step (1) of crushing the raw materials before the drying treatment is carried out, wherein the drying treatment time is 3-4 h.

Further, the extraction solvent used in the flash extraction is water, and the addition amount of the extraction solvent is 12-16 times of the weight of the pretreated raw materials.

Further, the flash extraction time is 1-1.5min, and the extraction temperature is 55-65 deg.C.

Further, the concentration in the steps (4) and (5) is carried out in a flash concentrator, the vacuum degree during concentration is-0.7 to-0.9 MPa, the temperature is 55 to 65 ℃, and the concentration time is 15 to 20 s.

Further, the organic solvent in the step (5) is n-hexane, and the temperature of vacuum drying is 45-55 ℃.

The invention discloses the following technical effects:

the blood sugar-reducing and weight-losing traditional Chinese medicine composition disclosed by the invention is reasonable in compatibility, fully retains the active ingredients of the medicine by utilizing a flash extraction mode, is a safe and effective traditional Chinese medicine blood sugar-reducing medicine, and has the effect of reducing weight on the basis of effectively controlling the content of blood sugar.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a morphology chart of each group of mice after 12 weeks of rearing, wherein a is Normal group, b is Control group, and c is Sample group;

FIG. 2 is a weight chart of each group of mice at 0-12 weeks;

FIG. 3 is the average of weekly food intake for each mouse in each group during the experiment;

FIG. 4 is a graph of weight gain for each group of mice;

FIG. 5 is a graph of food efficacy ratios for each group;

FIG. 6 is a graph showing the fat content in the dry feces of mice in week 2, week 5, and week 10 high fat diet group and treatment group;

FIG. 7 is a graph showing the total serum cholesterol levels of various groups of mice;

FIG. 8 is a graph showing the serum triglyceride content of each group of mice;

FIG. 9 is a graph of serum HDL and serum LDL/VLDL, where a is a graph of serum HDL content and b is a graph of serum LDL/VLDL;

FIG. 10 is a graph of liver weights of various groups of mice;

FIG. 11 is a pictorial view of livers of various groups of mice; wherein, a is Normal group, b is Control group, c is Sample group;

FIG. 12 is a graph showing the weight of white adipose tissue, kidney tissue and spleen tissue in each group of mice; wherein a is a weight graph of white adipose tissue, b is a weight graph of kidney tissue, and c is a weight graph of spleen tissue;

FIG. 13 is a 20-fold magnified microscopic image of liver tissue of each group of mice; wherein, a is Normal group, b is Control group, c is Sample group.

FIG. 14 is a 20-fold magnified microscopic image of white adipose tissue of each group of mice; wherein, a is Normal group, b is Control group, c is Sample group.

FIG. 15 is a microscope photograph of each group of mice at 10-fold enlargement of kidney tissue; wherein, a is Normal group, b is Control group, c is Sample group.

FIG. 16 is a 20-fold magnified microscopic image of kidney tissue from each group of mice; wherein, a is Normal group, b is Control group, c is Sample group.

FIG. 17 is a microscope photograph of spleen tissue of each group of mice at 10-fold magnification; wherein, a is Normal group, b is Control group, c is Sample group.

FIG. 18 is a 20-fold magnified micrograph of spleen tissue from each group of mice; wherein, a is Normal group, b is Control group, c is Sample group.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

The "parts" in the present invention are all parts by mass unless otherwise specified.

Example 1

A traditional Chinese medicine preparation for losing weight and reducing blood sugar comprises the following components in parts by weight:

23 parts of Chinese yam, 8 parts of black beans, 12 parts of red beans, 11 parts of bitter gourds, 13 parts of trichosanthes roots, 16 parts of jerusalem artichoke and 16 parts of radix acanthopanacis senticosi.

The preparation method comprises the following steps:

(1) pretreatment of raw materials: weighing the raw materials according to the weight part ratio, crushing the raw materials to 200 meshes, and then drying the powder for 3 hours at the temperature of 45 ℃;

(2) flash extraction: adding water 12 times the weight of the pretreated raw materials, uniformly mixing, transferring to a flash extractor, extracting at 55 ℃ for 1min, filtering, and collecting filtrate; extracting the residue after filtration by the same method, and combining the filtrates;

(3) centrifuging the filtrate: centrifuging the obtained filtrate at 12 deg.C and 2500r/min for 5min, and collecting supernatant;

(4) concentration: placing the supernatant in a flash concentrator, and concentrating for 15s at 55 deg.C and-0.7 MPa to obtain extract;

(5) and (3) purification: adding water into the extract obtained in the step (4) for suspension, extracting by using normal hexane, removing an organic layer, placing a water phase into a flash concentrator, concentrating for 20s under the conditions that the vacuum degree is-0.7 MPa and the temperature is 55 ℃, and drying the concentrated product in vacuum at 55 ℃ to obtain a powdery product.

Example 2

27 parts of Chinese yam, 12 parts of black beans, 8 parts of phaseolus calcaratus, 9 parts of bitter gourds, 14 parts of trichosanthes roots, 14 parts of jerusalem artichoke and 13 parts of acanthopanax roots.

The preparation method comprises the following steps:

(1) pretreatment of raw materials: weighing the raw materials according to the weight part ratio, crushing the raw materials to 300 meshes, and then drying the powder for 4 hours at 50 ℃;

(2) flash extraction: adding water 16 times the weight of the pretreated raw materials, uniformly mixing, transferring to a flash extractor, extracting at 60 ℃ for 1.5min, filtering, and collecting filtrate; extracting the residue after filtration by the same method, and combining the filtrates;

(3) centrifuging the filtrate: centrifuging the obtained filtrate at 10 deg.C and 3000r/min for 6min, and collecting supernatant;

(4) concentration: placing the supernatant in a flash concentrator, and concentrating for 20s at vacuum degree of-0.8 MPa and temperature of 60 deg.C to obtain extract;

(5) and (3) purification: adding water into the extract obtained in the step (4) for suspension, extracting by using normal hexane, removing an organic layer, placing a water phase into a flash concentrator, concentrating for 15s under the conditions that the vacuum degree is-0.9 MPa and the temperature is 60 ℃, and drying the concentrated product in vacuum at 45 ℃ to obtain a powdery product.

Example 3

The preparation method comprises the following steps:

(1) pretreatment of raw materials: weighing the raw materials according to the weight part ratio, crushing the raw materials into 250 meshes, and then drying the powder for 3 hours at the temperature of 48 ℃;

(2) flash extraction: adding water 15 times the weight of the pretreated raw materials, uniformly mixing, transferring to a flash extractor, extracting at 65 ℃ for 1min, filtering, and collecting filtrate; extracting the residue after filtration by the same method, and combining the filtrates;

(3) centrifuging the filtrate: centrifuging the obtained filtrate at 11 deg.C and 2000r/min for 5min, and collecting supernatant;

(4) concentration: placing the supernatant in a flash concentrator, and concentrating for 18s under the conditions of vacuum degree of-0.9 MPa and temperature of 65 ℃ to obtain extract;

(5) and (3) purification: adding water into the extract obtained in the step (4) for suspension, extracting by using normal hexane, removing an organic layer, placing a water phase into a flash concentrator, concentrating for 18s under the conditions that the vacuum degree is-0.8 MPa and the temperature is 65 ℃, and drying the concentrated product in vacuum at 50 ℃ to obtain a powdery product.

First, in vitro experiment

The Chinese medicinal preparation prepared in example 1 was prepared into solutions of different concentrations, and the antioxidant activity thereof was measured.

The results of the DPPH free radical scavenging activity of the Chinese medicinal preparations with different concentrations are shown in Table 1; the half inhibitory concentrations of the herbal formulations against DPPH and ABTS are shown in Table 2.

TABLE 1DPPH radical scavenging Activity

TABLE 2 semi-inhibitory concentrations of DPPH and ABTS free radicals

Second, in vivo experiment

16 healthy C57BL/6 mice of the same growth period were divided into 3 groups, 5 mice on a Normal diet (Normal group), 6 mice on a high fat diet (Control group), and 5 mice on a treatment group (Sample group), and were kept under the same experimental environment during which the mice were freely supplied with food and water. The composition of the feed for the mice in the normal diet group is shown in table 3, the composition of the feed for the mice in the treatment group and the mice in the high-fat diet group are fed with the high-fat diet, the composition of the feed is shown in table 4, and the nutritional composition of the feed is shown in table 5. The treatment groups were administered twice daily by gavage (the Chinese medicinal preparation of example 1) at a dose of 500 mg/kg. The body weight and food intake of mice were measured weekly during the experiment for 12 weeks.

TABLE 3 Normal diet component composition

TABLE 4 high fat diet composition

TABLE 5 feed Nutrition ingredients

(1) Morphology of mouse

After 12 weeks of feeding, morphology of each group of mice was observed, and the results are shown in fig. 1.

Wherein, a is Normal group, b is Control group, c is Sample group. As can be seen from figure 1, the Chinese medicinal preparation for reducing blood sugar and losing weight can inhibit the fatness of mice.

(2) Body weight of mouse

The body weight of each group of mice for each week during the experiment is shown in figure 2. Note: data show relative mean ± standard deviation, statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.05; p < 0.01, P < 0.001vs Control.

As can be seen from fig. 2, the preparation of the present invention can control the weight gain of mice compared to the group with high fat diet.

(3) Food intake in mice

The average of the weekly food intake of each mouse in each group during the experiment is shown in figure 3.

(4) Weight gain and food efficacy ratio in mice

The weight gain of each group of mice is shown in figure 4, and the food efficacy ratio is shown in figure 5.

As can be seen from fig. 4, the treated mice grew less rapidly in weight than the high fat diet group; as can be seen from fig. 5, the food efficacy ratio was relatively low in the treatment group compared to the high fat diet group.

Note: data show relative mean ± standard deviation, statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.05; p < 0.01, P < 0.001vs Control.

(5) Fecal fat excretion in mice

The experimental method comprises the following steps: collecting feces of mice in high fat diet group and treatment group at 2, 5 and 10 weeks of feeding mice, collecting 0.5-1g of dry feces, adding 3ml of water, moisturizing, and adding methanol: the fecal fat was extracted from the chloroform-1: 2 mixed solution and the fat content in the dry feces was calculated and the results are shown in figure 6.

As can be seen from FIG. 6, the fat content in the dry feces of the mice in the high-fat diet group and the treatment group is not much different at

weeks

2 and 5, and the fat content in the dry feces of the mice in the treatment group is obviously higher than that of the mice in the high-fat diet group at week 10, which shows that the traditional Chinese medicine preparation of the invention can inhibit the absorption of the fat in the feed by the mice.

(6) Blood glucose levels in mice

Blood glucose levels were measured in each group of mice at

weeks

0, 3, 6, 9, and 12 of the experiment and the results are shown in table 6.

TABLE 6

Note: data show relative mean ± standard deviation, statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.05; p < 0.01; p < 0.001vs Control.

As can be seen from table 6, blood glucose was effectively controlled in the treated mice compared to the high fat diet group.

(7) Determination of serum insulin and Steady State model evaluation (HOMA) -Insulin Resistance (IR)

The experimental method comprises the following steps: (ii) performing fasting blood glucose test every 3 weeks with a one-touch blood glucose monitoring system

N, i-sens, Korea). At the end of the experiment, serum samples were prepared after centrifugation of the collected blood samples (at 14000 rpm for 20 minutes at 4 ℃) and stored at-70 ℃ for biochemical analysis. Mouse insulin was tested using a mouse insulin ELISA kit (Cat # 638-. And evaluating insulin resistance (HOMA-IR) according to a steady-state model, and calculating HOMA-IR by taking fasting blood glucose at the sacrifice day as an index, wherein the HOMA-IR is shown as a formula (1).

HOMA-IR [ { fasting insulin (μ IU/ml) } × { fasting blood glucose (mmol/l) }/22.5 formula (1)

The results are shown in Table 7.

TABLE 7

(8) Analysis of blood lipid profiles

The levels of serum Total Cholesterol (TC), High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), cholesterol and triglycerides were determined using respective bioassay kits (BioVision, Milpitas, USA) according to the manufacturer's procedures.

FIG. 7 shows the serum total cholesterol levels of various groups of mice; FIG. 8 is serum triglyceride levels;

FIG. 9 is a graph showing serum HDL and serum LDL/VLDL, in which a is a graph showing the serum HDL content and b is a graph showing the serum LDL/VLDL.

The data in fig. 7, 8 and 9 show relative mean ± standard deviation, and statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.05; p < 0.001vs Control.

From fig. 7-9, it can be seen that the treatment group can significantly reduce serum total cholesterol, reduce serum triglyceride, increase serum high density lipoprotein, and reduce serum LDL/VLDL, compared with the high fat diet group, which indicates that the traditional Chinese medicine preparation of the present invention has the effect of reducing blood fat.

(9) Atherosclerotic index and protection rate

The atherosclerosis index and the protection rate were determined for each group and the results are shown in Table 8.

TABLE 8

Wherein, the calculation formula of the Atherosclerosis Index (AI) is shown as the formula (2):

the formula for calculating the protection rate is shown in formula (3):

as can be seen from table 8, the treatment group reduced the atherosclerotic index with a 7.12% protection rate compared to the high fat diet group.

(10) Weight of liver, white adipose tissue, kidney tissue and spleen tissue of each group of mice

The liver weights of the mice in each group are shown in FIG. 10. Data show relative mean ± standard deviation, statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.05; p < 0.01 vsControl.

FIG. 11 is a schematic diagram of the livers of each group of mice, wherein a is the Normal group, b is the Control group, and c is the Sample group.

The weights of white adipose tissues, kidney tissues and spleen tissues of each group of mice were measured, and the measurement results are shown in FIG. 12.

Wherein a is a weight graph of white adipose tissues of each group of mice, b is a weight graph of kidney tissues of each group of mice, and c is a weight graph of spleen tissues of each group of mice.

Statistical significance was calculated using one-way analysis of variance and Dunnett's multiple comparison test. P < 0.001vs Control.

Thirdly, the liver tissue, the white adipose tissue, the kidney tissue and the spleen tissue of the experimental mouse are stained by adopting an eosin staining method

As can be seen from fig. 13 to 18, the lipid content in the liver, kidney and spleen of the mice in the drug group was significantly reduced, and the size of adipocytes was significantly reduced, indicating that the traditional Chinese medicine preparation of the present invention can promote lipid metabolism.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. The traditional Chinese medicine composition for reducing blood sugar and losing weight is characterized by comprising the following raw materials in parts by weight:

2. The traditional Chinese medicine composition according to claim 1, wherein the raw materials comprise the following components in parts by weight:

3. A Chinese medicinal preparation for reducing blood sugar and body weight, which comprises the extract of the Chinese medicinal composition of claim 1 or 2.

4. The Chinese medicinal preparation as claimed in claim 3, wherein the extraction method of the Chinese medicinal composition extract comprises the following steps:

(1) pretreatment of raw materials: drying the raw materials at 45-50 ℃;

(4) concentration: concentrating the supernatant to obtain extract;

5. The Chinese medicinal preparation according to claim 4, wherein the drying treatment in step (1) further comprises a step of pulverizing the raw materials, and the drying treatment lasts for 3-4 h.

6. The Chinese medicinal preparation according to claim 4, wherein the extraction solvent used in the flash extraction is water, and the amount of the extraction solvent is 12-16 times of the weight of the pretreated raw materials.

7. The Chinese medicinal preparation according to claim 4, wherein the flash extraction time is 1-1.5min and the extraction temperature is 55-65 ℃.

8. The Chinese medicinal preparation according to claim 4, wherein the concentration in steps (4) and (5) is carried out in a flash concentrator, the vacuum degree during concentration is-0.7 to-0.9 MPa, the temperature is 55 to 65 ℃, and the concentration time is 15 to 20 s.

9. The Chinese medicinal preparation according to claim 4, wherein the organic solvent in step (5) is n-hexane, and the temperature for vacuum drying is 45-55 ℃.