CN113405848A - Aquatic pathogenic microorganism collection device - Google Patents
Aquatic pathogenic microorganism collection device Download PDFInfo
- Publication number
- CN113405848A CN113405848A CN202110665670.5A CN202110665670A CN113405848A CN 113405848 A CN113405848 A CN 113405848A CN 202110665670 A CN202110665670 A CN 202110665670A CN 113405848 A CN113405848 A CN 113405848A
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- CN
- China
- Prior art keywords
- test tube
- box
- pathogenic microorganism
- sample
- bottom plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000000010 microbial pathogen Species 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000005070 sampling Methods 0.000 abstract description 9
- 238000004321 preservation Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 235000011089 carbon dioxide Nutrition 0.000 description 5
- 238000001914 filtration Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 3
- 239000011435 rock Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a water body pathogenic microorganism collecting device which comprises an outer box and an inner box, wherein a gap is formed between the outer box and the inner box, a box cover is matched with the top of the outer box, a test tube rack is placed in the inner box and comprises a lower bottom plate and an upper bottom plate, a group of test tube holes are uniformly distributed in the upper bottom plate, a group of test tube bottom grooves are uniformly distributed in the lower bottom plate, test tubes are inserted in the test tube holes, the bottoms of the test tubes are placed in the test tube bottom grooves, the test tube rack is matched with a disposable sample divider, the disposable sample divider comprises a sample dividing box, a funnel is arranged at the top of the sample dividing box, a filter screen is arranged in the funnel, and a group of sample dividing tubes are uniformly distributed at the bottom of the sample dividing box. The invention has reasonable design, is convenient for realizing mass sampling and has good preservation effect.
Description
Technical Field
The invention relates to the technical field of microorganism sampling, in particular to a water body pathogenic microorganism collecting device.
Background
When the pathogenic microorganisms in the water body are detected, the water body needs to be sampled and collected, and aseptic operation is required to be paid attention to water sample collection so as to prevent mixed bacteria from being mixed. And (4) selecting a filter membrane with a proper pore diameter according to the experimental setting, and filtering the water body. For turbid water, the method is recommended to be used for standing and separating suspended particles before filtration, and the suspended particles are firstly filtered by a large-aperture filter membrane and then filtered by a small-aperture filter membrane. After the sample information is marked, the sample information is quickly frozen by liquid nitrogen or placed in a foam box filled with dry ice, and the sample information is transported back to a laboratory as soon as possible in a dark environment and stored in a refrigerator at the temperature of minus 80 ℃, and then the sample information is sent by the dry ice.
At present, most of existing water body pathogenic microorganism collecting devices are simple in structure, poor in preservation effect, difficult to complete a large number of samples, and limited in sample quantity and relatively low in detection data reliability. Therefore, there is a need for a novel collection of aquatic pathogenic microorganisms that overcomes the above-mentioned deficiencies.
Disclosure of Invention
The invention aims to provide a water body pathogenic microorganism collecting device to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a water body pathogenic microorganism collection device, includes outer container and inner box, there is the clearance between outer container and the inner box, the top cooperation of outer container has the case lid, the test-tube rack has been placed in the inner box, the test-tube rack includes lower plate and upper plate, evenly distributed has a set of test tube hole on the upper plate, evenly distributed has a set of test tube kerve on the lower plate, the test tube has been pegged graft in the test tube hole, the bottom of test tube is placed in the test tube kerve, the test-tube rack cooperation has disposable riffle sampler, disposable riffle sampler is including dividing the appearance box, it is provided with the funnel to divide appearance box top, be provided with the filter screen in the funnel, the bottom evenly distributed who divides the appearance box has a component appearance pipe.
Preferably, the edge of the upper end face of the upper base plate is provided with a step, a frame is arranged around the bottom of the sample distribution box, and the frame is in clamping fit with the step.
Preferably, the upper port department of outer container is provided with the joint mouth, case lid lower extreme and joint mouth joint cooperation, joint mouth bottom is provided with sealed the pad.
Preferably, the outer box and the box cover are arranged to be of a double-layer structure, and vacuum is formed between the inner wall and the outer wall of the outer box and the inner wall and the outer wall of the box cover.
Preferably, an elastic pad is bonded on the inner wall of the top of the box cover.
Preferably, the upper end of the outer wall around the outer box is provided with a locking block, the lower end of the outer wall around the box cover is provided with a lock catch, and the lock catch is in clamping fit with the locking block.
Preferably, a handle is arranged on the side wall of the upper bottom plate, and a sponge pad is adhered to the inner wall of the test tube hole.
Preferably, a handle is arranged at the top of the box cover.
Compared with the prior art, the invention has the beneficial effects that: during sampling, dry ice is filled in a gap between the outer box and the inner box, the obtained water body is loaded into a test tube through the disposable sample splitter, the port of the test tube is sealed, and then the box cover is closed. The disposable riffle sampler effectively shortens the time of water body in the test tube, has reduced the contaminated risk of water body, is convenient for realize a large amount of samplings simultaneously. The vacuum outer box and the box cover have better heat preservation performance. Cover the case lid behind the sealed test tube, the cushion is pressed and is established in the test tube upper end, avoids the test tube to rock on the way in the transportation. The sponge liner prevents the test tube from being damaged in the taking and placing process. The invention has reasonable design, is convenient for realizing mass sampling and has good preservation effect.
Drawings
FIG. 1 is a schematic structural diagram of a water body pathogenic microorganism collecting device;
FIG. 2 is an enlarged view of a portion of FIG. 1 at K;
FIG. 3 is a schematic structural diagram of a sampler in the water body pathogenic microorganism collecting device;
FIG. 4 is a schematic view of the bottom structure of a sampler in a water pathogenic microorganism collecting device.
In the figure: 1-an outer box, 2-an inner box, 3-a box cover, 4-a clamping interface, 5-a sealing gasket, 6-an elastic cushion, 7-a locking block, 8-a lock catch, 9-a handle, 10-a test tube rack, 11-an upper base plate, 12-a test tube hole, 13-a lower base plate, 14-a test tube bottom groove, 15-a test tube, 16-a step, 17-a handle, 18-a disposable sample divider, 19-a sample dividing box, 20-a frame, 21-a sample dividing tube, 22-a funnel and 23-a filter screen.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 4, the present invention provides a technical solution:
the utility model provides a water body pathogenic microorganism collection device, includes outer container 1 and inner box 2, there is the clearance between outer container 1 and the inner box 2, the top cooperation of outer container 1 has case lid 3, place test-tube rack 10 in the inner box 2, test-tube rack 10 includes bottom plate 13 and upper plate 11, evenly distributed has a set of test tube hole 12 on the upper plate 11, evenly distributed has a set of test tube kerve 14 on the bottom plate 13, test tube 15 has been pegged graft in the test tube hole 12, test tube 15's bottom is placed in test tube kerve 14, test-tube rack 10 cooperates disposable riffle sampler 18, disposable riffle sampler 18 is including dividing sample box 19, divide sample box 19 top to be provided with funnel 22, be provided with filter screen 23 in the funnel 22, divide the bottom evenly distributed of sample box 19 to have a group to divide sample pipe 21.
During sampling, dry ice is filled in a gap between the outer box 1 and the inner box 2, the obtained water body is loaded into the test tube 15 through the disposable sample splitter 18, the port of the test tube 15 is sealed, and the box cover 3 is closed. The disposable sample splitter 18 can be used immediately after being unsealed, cannot be reused, is recycled, and needs to be disinfected and cleaned at a later stage. The water body flows into the sample dividing box 19 through the funnel 22 and then flows into the test tube 15 through the sample dividing tube 21, and the filter screen 23 is used for filtering impurities in the water body. The disposable sample splitter 18 effectively shortens the time for loading the water body into the test tube 15, reduces the risk of water body pollution, and is convenient for realizing mass sampling.
Preferably, a step 16 is arranged at the edge of the upper end face of the upper bottom plate 11, a frame 20 is arranged around the bottom of the sample distribution box 19, and the frame 20 is in clamping fit with the step 16.
Preferably, the upper port department of outer container 1 is provided with joint interface 4, 3 lower extremes of case lid and the cooperation of 4 joints of joint interface, 4 bottoms of joint interface are provided with sealed pad 5.
The gasket 5 ensures the sealing between the outer case 1 and the case cover 3.
Preferably, the outer box 1 and the box cover 3 are configured as a double-layer structure, and a vacuum is formed between the inner and outer walls of the outer box 1 and the box cover 3.
The outer box 1 and the box cover 3 arranged in vacuum have better heat preservation performance.
Preferably, an elastic pad 6 is adhered to the inner wall of the top of the case cover 3.
Preferably, the upper end of the peripheral outer wall of the outer box 1 is provided with a locking block 7, the lower end of the peripheral outer wall of the box cover 3 is provided with a lock catch 8, and the lock catch 8 is in clamping fit with the locking block 7.
The lock catch 8 is matched with the lock block 7 in a clamping way to lock the box cover 3.
Preferably, the side wall of the upper base plate 11 is provided with a handle 17, and the inner wall of the test tube hole 12 is adhered with a sponge pad.
The sponge pad prevents the test tube 15 from being damaged in the taking and placing process.
Preferably, a handle 9 is provided on the top of the cover 3.
The handle 9 is convenient for screwing up the box body.
The working principle of the invention is as follows: during sampling, dry ice is filled in a gap between the outer box 1 and the inner box 2, the obtained water body is loaded into the test tube 15 through the disposable sample splitter 18, the port of the test tube 15 is sealed, and the box cover 3 is closed. The disposable sample splitter 18 can be used immediately after being unsealed, cannot be reused, is recycled, and needs to be disinfected and cleaned at a later stage. The water body flows into the sample dividing box 19 through the funnel 22 and then flows into the test tube 15 through the sample dividing tube 21, and the filter screen 23 is used for filtering impurities in the water body. The disposable sample splitter 18 effectively shortens the time for loading the water body into the test tube 15, reduces the risk of water body pollution, and is convenient for realizing mass sampling. Frame 20 and step 16 joint cooperation guarantee to divide appearance pipe 21 and test tube 15 to aim at. The gasket 5 ensures the sealing between the outer case 1 and the case cover 3. The outer box 1 and the box cover 3 arranged in vacuum have better heat preservation performance. Cover case lid 3 behind the sealed test tube 15, elastic pad 6 presses and establishes in test tube 15 upper end, avoids test tube 15 to rock on the way in the transportation. The sponge pad prevents the test tube 15 from being damaged in the taking and placing process.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. The utility model provides a water body pathogenic microorganism collection device, includes outer container (1) and inner box (2), its characterized in that: a gap is arranged between the outer box (1) and the inner box (2), the top of the outer box (1) is matched with a box cover (3), a test tube rack (10) is arranged in the inner box (2), the test tube rack (10) comprises a lower bottom plate (13) and an upper bottom plate (11), a group of test tube holes (12) are uniformly distributed on the upper bottom plate (11), a group of test tube bottom grooves (14) are uniformly distributed on the lower bottom plate (13), a test tube (15) is inserted into the test tube hole (12), the bottom of the test tube (15) is placed in the test tube bottom groove (14), the test tube rack (10) is matched with a disposable sample splitter (18), the disposable sample splitter (18) comprises a sample splitting box (19), a funnel (22) is arranged at the top of the sample separating box (19), a filter screen (23) is arranged in the funnel (22), the bottom of the sample separating box (19) is uniformly distributed with a group of sample separating tubes (21).
2. An aquatic pathogenic microorganism collecting apparatus according to claim 1, wherein: the edge of the upper end face of the upper base plate (11) is provided with a step (16), a frame (20) is arranged around the bottom of the sample distribution box (19), and the frame (20) is in clamping fit with the step (16).
3. An aquatic pathogenic microorganism collecting apparatus according to claim 2, wherein: the upper port department of outer container (1) is provided with joint interface (4), case lid (3) lower extreme and joint interface (4) joint cooperation, joint interface (4) bottom is provided with sealed pad (5).
4. An aquatic pathogenic microorganism collecting apparatus according to claim 3, wherein: outer container (1) and case lid (3) set up to bilayer structure, are the vacuum between the interior outer wall of outer container (1) and case lid (3).
5. An aquatic pathogenic microorganism collecting apparatus according to claim 4, wherein: an elastic pad (6) is bonded on the inner wall of the top of the box cover (3).
6. An aquatic pathogenic microorganism collecting apparatus according to claim 5, wherein: the outer container (1) is provided with locking piece (7) in the upper end of outer wall all around, the lower extreme of outer wall all around of case lid (3) is provided with hasp (8), hasp (8) and locking piece (7) joint cooperation.
7. An aquatic pathogenic microorganism collecting apparatus according to claim 6, wherein: the side wall of the upper bottom plate (11) is provided with a handle (17), and the inner wall of the test tube hole (12) is adhered with a sponge liner.
8. An aquatic pathogenic microorganism collecting apparatus according to claim 7, wherein: the top of the box cover (3) is provided with a handle (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110665670.5A CN113405848A (en) | 2021-06-16 | 2021-06-16 | Aquatic pathogenic microorganism collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110665670.5A CN113405848A (en) | 2021-06-16 | 2021-06-16 | Aquatic pathogenic microorganism collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113405848A true CN113405848A (en) | 2021-09-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110665670.5A Pending CN113405848A (en) | 2021-06-16 | 2021-06-16 | Aquatic pathogenic microorganism collection device |
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| Country | Link |
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| CN (1) | CN113405848A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205653454U (en) * | 2016-05-17 | 2016-10-19 | 南阳师范学院 | Cell culture base filter equipment |
| CN108216856A (en) * | 2017-12-21 | 2018-06-29 | 成都汇鲤科技有限公司 | A kind of novel water body microbial diversity monitoring sample cartridge |
| CN208292998U (en) * | 2018-04-10 | 2018-12-28 | 云南精赛顿生物科技有限公司 | A kind of liquid subpackage mobile device |
| CN210175442U (en) * | 2019-06-11 | 2020-03-24 | 广东以勒咨询有限公司 | Microorganism water sample transportation and storage device |
| CN111896315A (en) * | 2020-06-22 | 2020-11-06 | 淮南钦鼎生物科技有限公司 | Rapid detection method for environmental water microorganisms |
| CN212987728U (en) * | 2020-07-13 | 2021-04-16 | 张建设 | Sewage detection sample storage device |
| CN113462539A (en) * | 2021-07-01 | 2021-10-01 | 枣庄科技职业学院 | Pathogenic microorganism separation and extraction device |
-
2021
- 2021-06-16 CN CN202110665670.5A patent/CN113405848A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205653454U (en) * | 2016-05-17 | 2016-10-19 | 南阳师范学院 | Cell culture base filter equipment |
| CN108216856A (en) * | 2017-12-21 | 2018-06-29 | 成都汇鲤科技有限公司 | A kind of novel water body microbial diversity monitoring sample cartridge |
| CN208292998U (en) * | 2018-04-10 | 2018-12-28 | 云南精赛顿生物科技有限公司 | A kind of liquid subpackage mobile device |
| CN210175442U (en) * | 2019-06-11 | 2020-03-24 | 广东以勒咨询有限公司 | Microorganism water sample transportation and storage device |
| CN111896315A (en) * | 2020-06-22 | 2020-11-06 | 淮南钦鼎生物科技有限公司 | Rapid detection method for environmental water microorganisms |
| CN212987728U (en) * | 2020-07-13 | 2021-04-16 | 张建设 | Sewage detection sample storage device |
| CN113462539A (en) * | 2021-07-01 | 2021-10-01 | 枣庄科技职业学院 | Pathogenic microorganism separation and extraction device |
Non-Patent Citations (1)
| Title |
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| 王晓斐等: "固定化酵母利用甘蔗汁发酵酒精工艺条件的研究", 《食品与发酵科技》 * |
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Application publication date: 20210917 |
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| RJ01 | Rejection of invention patent application after publication |