CN111896315A - Rapid detection method for environmental water microorganisms - Google Patents
Rapid detection method for environmental water microorganisms Download PDFInfo
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- CN111896315A CN111896315A CN202010576352.7A CN202010576352A CN111896315A CN 111896315 A CN111896315 A CN 111896315A CN 202010576352 A CN202010576352 A CN 202010576352A CN 111896315 A CN111896315 A CN 111896315A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B63—SHIPS OR OTHER WATERBORNE VESSELS; RELATED EQUIPMENT
- B63B—SHIPS OR OTHER WATERBORNE VESSELS; EQUIPMENT FOR SHIPPING
- B63B35/00—Vessels or similar floating structures specially adapted for specific purposes and not otherwise provided for
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
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Abstract
The invention belongs to the technical field of microorganism detection, and particularly relates to a rapid detection method for microorganisms in an environmental water body.A sampling device used in the method comprises a first frame and a second frame, wherein two rotating shafts are rotatably arranged in the first frame, a driving wheel is fixed on the surface of each rotating shaft, two driven wheels are rotatably arranged in the second frame, a driving motor is fixed on the side wall of the first frame, a chain is movably connected between the output shaft end of the driving motor and the rotating shafts, the chain is used for driving the driving wheels to rotate, a water taking tank is fixed on the side wall of the second frame, through holes are formed in the top end and the bottom end of the water taking tank, and a straight rod is movably arranged in the water taking tank; according to the invention, the driving motor drives the sampling device to vertically lift along the rope entering the water body, so that not only is safe water taking of the deep well completed, but also the water sample is filtered in the water taking process, thus the safety in the water taking process is ensured, and the accuracy of water sample detection is also improved.
Description
Technical Field
The invention belongs to the technical field of microorganism detection, and particularly relates to a rapid detection method for microorganisms in an environmental water body.
Background
The water environment refers to the environment of the space where water is formed, distributed and transformed in nature. Refers to the totality of various natural factors and related social factors surrounding the space of people and water bodies which can directly or indirectly influence the life and development of human beings. There are also environments in which natural waters, bounded by land, are located that are relatively stable. The microorganisms are lower organisms which are widely existed in nature, have tiny, large quantity and invisible to naked eyes and can be observed only by magnifying by hundreds of times or thousands of times by means of an optical microscope or an electron microscope
Some technical schemes related to microorganism detection also appear in the prior art, for example, a Chinese patent with application number of 200410026795X discloses a method for rapidly detecting microorganisms in an environmental water body, firstly, a water sample is filtered by microporous filter membranes with different apertures from large to small in sequence, and then the filter membranes are taken down for ATP luminescence detection; and simultaneously carrying out the luminescence detection of the ATP standard solution and the blank microporous filter membrane, and making a linear regression equation according to the logarithmic relation of the ATP concentration and the luminescence pulse counting CPM value: lg [ ATP ] ═ a + BLg [ CPM ]; and finally, the ATP concentration of the sample and the content of the microorganisms in the water body are calculated according to the CPM value of the filter membrane, and the conventional sample sampling method is adopted in the patent, so that the sample in a deep well or a sky pit is difficult to be quickly sampled, and the progress of microorganism detection is influenced.
Therefore, the invention provides a method for rapidly detecting microorganisms in an environmental water body, wherein the sampling device used in the method drives the sampling device to vertically lift along a rope entering the water body through a driving motor, so that not only is the safe water taking of a deep well completed, but also the water sample is filtered in the water taking process, thereby ensuring the safety in the water taking process and improving the accuracy of water sample detection.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides the method for rapidly detecting the microorganisms in the environmental water body.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a method for rapidly detecting microorganisms in an environmental water body, which comprises the following steps:
s1: putting one end of a rope into a water body, penetrating one end of the rope, far away from the water body, into a sampling device, fixing the rope, starting the sampling device to sink the sampling device into the water body, driving a driving wheel to move by a driving motor in the moving process of the sampling device so as to enable the sampling device to move downwards, floating a buoyancy ball upwards to enable a straight rod to be driven upwards after the sampling device enters the water body, and feeding water into the bottom of a water taking tank to finish water taking; the water taking tank is driven to quickly enter the water body through the movement of the sampling device, personnel do not need to go deep into the underground or the bottom of a pit, so that accidents of the personnel are avoided, tools such as a deep water pump and the like are not needed, and the water taking is more convenient in the field;
s2: when the sampling device in the S1 finishes taking water, the driving motor rotates reversely, the driving wheel is driven by the driving motor to rotate along the surface of the rope to drive the sampling device to move upwards, the straight rod descends after the buoyancy ball floats out of the water surface, the water sample in the water taking tank is filtered by the filter screen on the straight rod until the straight rod seals the water taking tank, and the sampling device returns to the hand of a user under the action of the driving motor; the sampling device moves rapidly along the rope, so that the collecting speed of a water sample is improved, and meanwhile, the sampling device filters the water sample, so that the detection speed of the water sample is accelerated, the subsequent filtering steps are reduced, and the accuracy of water body detection is improved;
s3: pouring the filtered water sample in the water tank in S2 into a test tube, pouring a detection reagent into the test tube, and judging the microorganisms through the color change of the water sample; the water sample poured out of the water taking box is the filtered water sample, and secondary treatment is not needed, so that the detection speed of the water sample is improved, and the accuracy of detection is further improved;
wherein, the sampling device used in S1 and S2 comprises a first frame and a second frame, two rotating shafts are rotatably arranged in the first frame, the surfaces of the rotating shafts are fixedly provided with driving wheels, two driven wheels are rotatably arranged in the second frame, a driving motor is fixed on the side wall of the first frame, a chain is movably connected between the output shaft end of the driving motor and the rotating shafts and used for driving the driving wheels to rotate, the side wall of the second frame is fixedly provided with a water taking tank, the top end and the bottom end of the water taking tank are both provided with through holes, a straight rod is movably arranged in the water taking tank and in clearance fit with the through holes, the top end of the straight rod is provided with a buoyancy ball, the bottom of the straight rod is provided with a filter screen, the bottom of the water taking tank is provided with a filter grid plate, the side wall of the water taking tank is provided with a waterproof plug, the second, the controller is used for controlling the sampling device to work; when the water sampling device is used, when water samples in special terrains such as deep wells, sky pits and the like are collected, workers need to move to the water surface or put in a water taking bucket for sampling, on one hand, the threats are easily brought to the workers, on the other hand, the water taking time is long due to a manual water taking mode, and the influence on a detection structure is easily brought, so that the problem is solved; before water is taken, the rope is placed in a well or under a sky pit, then the free end of the rope penetrates between a driving wheel and a driven wheel of a sampling device, and the free end of the rope is fixed on a wall body or solid objects such as trees and the like; the working personnel put down the sampling device along the direction of the rope, and a driving motor on the sampling device drives a driving wheel to rotate under the control of a controller, so that the driving wheel drives the sampling device to move downwards; when the sampling device enters the water body, the sampling device continues to descend, and when the buoyancy ball enters the water body, the buoyancy ball floats upwards, so that the straight rod is driven upwards, and the water begins to enter the bottom of the water taking tank; when the water sampling tank is filled with a water sample, the sampling device moves upwards, and the straight rod moves downwards under the action of gravity after the buoyancy ball floats out of the water body, so that the two through holes are sealed by the straight rod, and the water sample in the water sampling tank cannot flow out; meanwhile, the straight rod moves downwards, so that the filter screen filters the water sample in the water taking tank once, and the water sample in the water taking tank is a water sample which can be directly detected; the driving motor drives the driving wheel to rotate so that the sampling device moves to an initial position to complete a water taking task; according to the invention, the driving motor drives the sampling device to vertically lift along the rope entering the water body, so that not only is safe water taking of the deep well completed, but also the water sample is filtered in the water taking process, thus the safety in the water taking process is ensured, and the accuracy of water sample detection is also improved; the water tank can sample the water body only when the water tank completely enters the water body, and the obtained water sample is water with a certain depth underwater, so that the effectiveness of sampling is guaranteed.
Preferably, the bottom end of the first frame is rotatably provided with two first discs, the bottom end of the second frame is rotatably provided with two second discs, the first discs are tightly attached to the adjacent second discs, the output shaft end of the driving motor is fixedly provided with a driving wheel, the driving wheel is tightly attached to one of the first discs, more than two blades are fixed between the two first discs and between the two second discs at equal intervals along the respective circumferential directions, and the blades are of arc structures; when the device is used, water plants may grow at the bottom of the deep well or the sky pit, so that the movement of the sampling device is hindered, and the collection of a water sample is influenced; the driving motor drives the driving wheel to rotate when working through the driving wheel, so that the driving wheel drives the first dial to rotate; the first disc is driven to rotate in the rotating process of the second disc, so that blades on the first disc and the second disc synchronously rotate; cutting aquatic weeds and foreign matters encountered in the advancing process of the sampling device in the rotating process of the blade, so that the sampling device can smoothly enter a water body; the blade is arc structure makes the clearance between blade and the rope reduce to the cutting range of blade has been improved.
Preferably, a cylinder is fixed between the first discs, a cylinder is fixed between the second discs, and rectangular plates are hinged to the surfaces of the two cylinders at equal intervals along the circumferential direction of the two cylinders; when the water sampler is used, the blades cannot drive away aquatic weeds and tree roots after cutting the aquatic weeds, the tree roots and other clothes in the water body, so that the aquatic weeds and the tree roots can easily enter the driving wheel to influence the movement of the sampling device; through being provided with the rectangular plate, drive the rectangular plate and rotate in a dish, No. two dish pivoted processes for the rectangular plate produces the collision with foreign matters such as showy pasture and water and root, thereby makes the foreign matter be knocked off by the rectangular plate, avoids the foreign matter to enter into the action wheel in, and then has guaranteed sampling device's normal removal.
Preferably, the top ends of the first frame and the second frame are respectively fixed with a support rod, the top ends of the support rods are provided with limiting holes, circular rings are movably mounted at the limiting holes, more than two propellers are fixed on the inner rings of the circular rings at equal intervals along the circumferential direction of the inner rings, the bottom end of the first frame is rotatably mounted with a circular rod, a worm is fixed on the surface of the circular rod, the worm is meshed with the circular rings, and a belt is movably connected between the output shaft end of the driving motor and the circular rod; when the worm-gear type ring gear is used, the worm is driven to rotate through the belt in the rotating process of the driving motor, so that the worm drives the ring to rotate; the propeller is driven to rotate in the process of rotating the circular ring; the sampling device can smoothly enter the water body through the rotation of the propeller in the process that the sampling device enters the water body, so that the problem that the sampling device cannot enter the water body due to the gas in the water taking tank is avoided; after the sampling device comes out of the water body, the propeller rotates to generate buoyancy, so that the gravity of the sampling device carrying a water sample is reduced, the workload of the driving motor is reduced, and the sampling device can smoothly return to the initial position.
Preferably, the rectangular plate and the adjacent blades are arranged in a staggered manner, water permeable holes are formed in the rectangular plate, and the density of one end, far away from the adjacent cylinder, of the rectangular plate is greater than that of the other end of the rectangular plate; when the water-permeable rectangular plate is used, the rectangular plate and the blades are arranged in a staggered manner, so that the distance of foreign matters cut by the blades, which are impacted by the rectangular plate, is increased, and meanwhile, the water-permeable holes in the rectangular plate enable the rectangular plate to move more smoothly in a water body, so that the water resistance of the rectangular plate is reduced; the rectangular plate is in a hinged state, and the density difference of the rectangular plate enables the rectangular plate to have larger impact force in the rotating process, so that the foreign matters are far away from the sampling device after being impacted by the rectangular plate.
Preferably, the first disc is of a cavity structure, and a water-absorbing resin is filled in an inner cavity of the first disc; when the water sampling device is used, the water taking tank has a certain weight after water is taken, so that the sampling device loses balance and the movement of the sampling device is influenced; make a dish in a absorb a large amount of moisture through be equipped with water absorbent resin in a dish to make sampling device keep balanced state, avoid causing the problem emergence that the rope was beaten by the screw because of sampling device's skew, and then guaranteed sampling device's normal removal.
The invention has the following beneficial effects:
1. according to the method for rapidly detecting the microorganisms in the environmental water body, the sampling device used in the method drives the sampling device to vertically lift along the rope entering the water body through the driving motor, so that not only is safe water taking of a deep well completed, but also the water sample is filtered in the water taking process, so that the safety in the water taking process is ensured, and the accuracy of water sample detection is also improved.
2. According to the method for rapidly detecting the microorganisms in the environmental water body, the blade is arranged on the sampling device used in the method to cut the foreign matters in the water body, so that the normal entering of the sampling device is ensured, the success rate of sampling is improved, and the normal recovery of the sampling device is also ensured.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a three-dimensional view of a sampling device used in the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is a right side view of a sampling device used in the present invention;
FIG. 5 is a schematic view of the working state of the sampling device used in the present invention;
in the figure: the water taking device comprises a first frame 1, a second frame 2, a driving wheel 3, a driven wheel 4, a driving motor 5, a chain 6, a water taking tank 7, a straight rod 8, a buoyancy ball 9, a filter screen 10, a filter grid plate 11, a waterproof plug 12, a first disc 13, a second disc 14, a driving wheel 15, a blade 16, a cylinder 17, a rectangular plate 18, a supporting rod 19, a circular ring 20, a propeller 21, a circular rod 22, a worm 23 and a belt 24.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 5, the method for rapidly detecting microorganisms in an environmental water body of the present invention comprises the following steps:
s1: putting one end of a rope into a water body, penetrating one end of the rope, far away from the water body, into a sampling device, fixing the rope, starting the sampling device to sink the sampling device into the water body, driving a driving wheel 3 to move by a driving motor 5 in the moving process of the sampling device so as to enable the sampling device to move downwards, floating a buoyancy ball 9 upwards after entering the water body so as to enable a straight rod 8 to be driven upwards, and feeding water from the bottom of a water taking tank 7 to finish water taking; the water taking tank 7 is driven to quickly enter the water body by the movement of the sampling device, and personnel do not need to go deep into the underground or the bottom of a pit, so that accidents of the personnel are avoided, tools such as a deep water pump and the like are not needed, and the water taking is more convenient in the field;
s2: when the sampling device in the step S1 finishes taking water, the driving motor 5 rotates reversely, the driving wheel 3 is driven by the driving motor 5 to rotate along the surface of the rope to drive the sampling device to move upwards, the straight rod 8 descends after the buoyancy ball 9 floats out of the water surface, the filter screen 10 on the straight rod 8 filters the water sample in the water taking tank 7 until the straight rod 8 seals the water taking tank 7, and the sampling device returns to the hand of the user under the action of the driving motor 5; the sampling device moves rapidly along the rope, so that the collecting speed of a water sample is improved, and meanwhile, the sampling device filters the water sample, so that the detection speed of the water sample is accelerated, the subsequent filtering steps are reduced, and the accuracy of water body detection is improved;
s3: pouring the filtered water sample in the water tank 7 in S2 into a test tube, pouring a detection reagent into the test tube, and judging the microorganisms through the color change of the water sample; the water sample poured out of the water taking tank 7 is the filtered water sample, and secondary treatment is not needed, so that the detection speed of the water sample is improved, and the accuracy of detection is further improved;
wherein, the sampling device used in S1 and S2 comprises a first frame 1 and a second frame 2, two rotating shafts are rotatably installed in the first frame 1, a driving wheel 3 is fixed on the surface of each rotating shaft, two driven wheels 4 are rotatably installed in the second frame 2, a driving motor 5 is fixed on the side wall of the first frame 1, a chain 6 is movably connected between the output shaft end of the driving motor 5 and the rotating shafts, the chain 6 is used for driving the driving wheel 3 to rotate, a water taking tank 7 is fixed on the side wall of the second frame 2, through holes are formed in the top end and the bottom end of the water taking tank 7, a straight rod 8 is movably arranged in the water taking tank 7, the straight rod 8 is in clearance fit with the through holes, a buoyancy ball 9 is arranged at the top end of the straight rod 8, a filter screen 10 is arranged at the bottom of the straight rod 8, a filter grid plate 11 is arranged at the bottom of the, the second frame 2 is provided with a storage battery and a controller, the storage battery is electrically connected with the controller, and the controller is used for controlling the sampling device to work; when the water sampling device is used, when water samples in special terrains such as deep wells, sky pits and the like are collected, workers need to move to the water surface or put in a water taking bucket for sampling, on one hand, the threats are easily brought to the workers, on the other hand, the water taking time is long due to a manual water taking mode, and the influence on a detection structure is easily brought, so that the problem is solved; before water is taken, the rope is placed in a well or under a pit, the free end of the rope penetrates between a driving wheel 3 and a driven wheel 4 of a sampling device, and the free end of the rope is fixed on a wall body or solid objects such as trees; the worker puts down the sampling device along the direction of the rope, and a driving motor 5 on the sampling device drives a driving wheel 3 to rotate under the control of a controller, so that the driving wheel 3 drives the sampling device to move downwards; when the sampling device enters the water body, the sampling device continuously descends, and when the buoyancy ball 9 enters the water body, the buoyancy ball floats upwards, so that the straight rod 8 is driven upwards, and the water begins to enter the bottom of the water taking tank 7; when the water sampling tank 7 is filled with a water sample, the sampling device moves upwards, and the straight rod 8 moves downwards under the action of gravity after the buoyancy ball 9 floats out of the water body, so that the straight rod 8 seals the two through holes, and the water sample in the water sampling tank 7 cannot flow out; meanwhile, in the process that the straight rod 8 moves downwards, the filter screen 10 filters the water sample in the water taking tank 7 once, so that the water sample in the water taking tank 7 is a water sample capable of being directly detected; the driving motor 5 drives the driving wheel 3 to rotate so that the sampling device moves to an initial position to finish a water taking task; according to the invention, the driving motor 5 drives the sampling device to vertically lift along the rope entering the water body, so that not only is safe water taking of the deep well completed, but also the water sample is filtered in the water taking process, thus the safety in the water taking process is ensured, and the accuracy of water sample detection is also improved; the water taking tank 7 can take samples of the water body only when completely entering the water body, and the obtained water sample is water with a certain depth underwater, so that the effectiveness of sampling is guaranteed.
As a specific embodiment of the present invention, two first disks 13 are rotatably mounted at the bottom end of the first frame 1, two second disks 14 are rotatably mounted at the bottom end of the second frame 2, the first disks 13 are tightly attached to the adjacent second disks 14, a driving wheel 15 is fixed at an output shaft end of the driving motor 5, the driving wheel 15 is tightly attached to one of the first disks 13, two or more blades 16 are fixed between the two first disks 13 and between the two second disks 14 at equal intervals along respective circumferential directions, and the blades 16 are in an arc structure; when the device is used, water plants may grow at the bottom of the deep well or the sky pit, so that the movement of the sampling device is hindered, and the collection of a water sample is influenced; by arranging the driving wheel 15, the driving motor 5 drives the driving wheel 15 to rotate when working, so that the driving wheel 15 drives the first dial 13 to rotate; the first disc 13 drives the second disc 14 to rotate in the rotating process, so that the blades 16 on the first disc 13 and the second disc 14 rotate synchronously; the water plants and foreign matters encountered in the advancing process of the sampling device are cut in the rotating process of the blade 16, so that the sampling device can smoothly enter a water body; the arc-shaped configuration of the blade 16 reduces the gap between the blade 16 and the strand, thereby increasing the cutting range of the blade 16.
As a specific embodiment of the present invention, a cylinder 17 is fixed between the two first disks 13, a cylinder 17 is fixed between the two second disks 14, and rectangular plates 18 are hinged on the surfaces of the two cylinders 17 at equal intervals along the circumferential direction; when the water sampler is used, the blades 16 can not drive away aquatic weeds and tree roots after cutting the aquatic weeds, tree roots and other clothes in the water body, so that the aquatic weeds and the tree roots can easily enter the driving wheel 3 to influence the movement of the sampling device; through being provided with rectangular plate 18, drive rectangular plate 18 and rotate in the pivoted in-process of a dish 13, No. two dishes 14 for rectangular plate 18 produces the collision with foreign matters such as showy pasture and water and root, thereby makes the foreign matter be knocked off by rectangular plate 18, avoids the foreign matter to enter into in the action wheel 3, and then has guaranteed sampling device's normal removal.
As a specific embodiment of the invention, a support rod 19 is fixed at the top ends of the first frame 1 and the second frame 2, a limit hole is arranged at the top end of the support rod 19, a circular ring 20 is movably installed at the limit hole, more than two propellers 21 are fixed at equal intervals along the circumferential direction of the inner ring of the circular ring 20, a circular rod 22 is rotatably installed at the bottom end of the first frame 1, a worm 23 is fixed on the surface of the circular rod 22, the worm 23 is meshed with the circular ring 20, and a belt 24 is movably connected between the output shaft end of the driving motor 5 and the circular rod 22; when the device is used, the worm 23 is arranged, and the worm 23 is driven to rotate through the belt 24 in the rotating process of the driving motor 5, so that the worm 23 drives the circular ring 20 to rotate; the ring 20 drives the propeller 21 to rotate in the rotating process; the sampling device can smoothly enter the water body through the rotation of the propeller 21 in the process that the sampling device enters the water body, so that the problem that the sampling device cannot enter the water body due to the gas in the water taking tank 7 is avoided; after the sampling device comes out of the water body, the propeller 21 generates buoyancy in the rotating process, so that the gravity of the sampling device carrying a water sample is reduced, the workload of the driving motor 5 is reduced, and the sampling device can smoothly return to the initial position.
As a specific embodiment of the present invention, the rectangular plate 18 is arranged in a staggered manner with the adjacent blades 16, the rectangular plate 18 is provided with water permeable holes, and the density of one end of the rectangular plate 18 away from the adjacent cylinder 17 is greater than that of the other end; when the water-saving filter is used, the rectangular plate 18 and the blades 16 are arranged in a staggered mode, so that the distance of foreign matters cut by the blades 16 impacted by the rectangular plate 18 is increased, meanwhile, the water-permeable holes in the rectangular plate 18 enable the rectangular plate 18 to move more smoothly in a water body, and the water resistance of the rectangular plate 18 is reduced; the rectangular plate 18 is hinged, and the density of the rectangular plate 18 is different, so that the rectangular plate 18 has a larger impact force in the rotating process, and foreign matters are far away from the sampling device after being impacted by the rectangular plate 18.
As a specific embodiment of the present invention, the first disc 13 has a cavity structure, and a cavity of the first disc 13 is filled with water-absorbent resin; when the water sampling device is used, the water taking tank 7 has a certain weight after water is taken, so that the sampling device loses balance and the movement of the sampling device is influenced; make a large amount of moisture of interior absorption of a dish 13 through be equipped with water absorbent resin in a dish 13 to make sampling device keep balanced state, avoid leading to the problem emergence that the rope was hit by screw 21 because of sampling device's skew, and then guaranteed sampling device's normal removal.
When the water sampling device is used, when water samples in special terrains such as deep wells, sky pits and the like are collected, workers need to move to the water surface or put in a water taking bucket for sampling, on one hand, the threats are easily brought to the workers, on the other hand, the water taking time is long due to a manual water taking mode, and the influence on a detection structure is easily brought, so that the problem is solved; before water is taken, the rope is placed in a well or under a pit, the free end of the rope penetrates between a driving wheel 3 and a driven wheel 4 of a sampling device, and the free end of the rope is fixed on a wall body or solid objects such as trees; the worker puts down the sampling device along the direction of the rope, and a driving motor 5 on the sampling device drives a driving wheel 3 to rotate under the control of a controller, so that the driving wheel 3 drives the sampling device to move downwards; when the sampling device enters the water body, the sampling device continuously descends, and when the buoyancy ball 9 enters the water body, the buoyancy ball floats upwards, so that the straight rod 8 is driven upwards, and the water begins to enter the bottom of the water taking tank 7; when the water sampling tank 7 is filled with a water sample, the sampling device moves upwards, and the straight rod 8 moves downwards under the action of gravity after the buoyancy ball 9 floats out of the water body, so that the straight rod 8 seals the two through holes, and the water sample in the water sampling tank 7 cannot flow out; meanwhile, in the process that the straight rod 8 moves downwards, the filter screen 10 filters the water sample in the water taking tank 7 once, so that the water sample in the water taking tank 7 is a water sample capable of being directly detected; the driving motor 5 drives the driving wheel 3 to rotate so that the sampling device moves to an initial position to finish a water taking task; according to the invention, the driving motor 5 drives the sampling device to vertically lift along the rope entering the water body, so that not only is safe water taking of the deep well completed, but also the water sample is filtered in the water taking process, thus the safety in the water taking process is ensured, and the accuracy of water sample detection is also improved; the water taking tank 7 can take samples of the water body only when completely entering the water body, and the obtained water sample is water with a certain depth underwater, so that the effectiveness of sampling is guaranteed.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A method for rapidly detecting microorganisms in an environmental water body is characterized by comprising the following steps:
s1: putting one end of a rope into a water body, penetrating one end of the rope, far away from the water body, into a sampling device, fixing the rope, starting the sampling device to sink the sampling device into the water body, driving a driving wheel (3) to move by a driving motor (5) in the moving process of the sampling device so as to enable the sampling device to move downwards, floating a buoyancy ball (9) after entering the water body so as to enable a straight rod (8) to be driven upwards, and feeding water from the bottom of a water taking tank (7) to finish water taking;
s2: when the sampling device in the S1 finishes taking water, the driving motor (5) rotates reversely, the driving wheel (3) is driven by the driving motor (5) to rotate along the surface of the rope to drive the sampling device to move upwards, the straight rod (8) descends after the buoyancy ball (9) floats out of the water surface, the filter screen (10) on the straight rod (8) filters the water sample in the water taking tank (7) until the straight rod (8) seals the water taking tank (7), and the sampling device returns to the hand of a user under the action of the driving motor (5);
s3: pouring the filtered water sample in the water tank (7) in S2 into a test tube, pouring a detection reagent into the test tube, and judging the microorganisms through the color change of the water sample;
wherein, the sampling device who uses in S1, S2 includes frame (1) and No. two frame (2), No. 1 internal rotation installs two pivots, pivot fixed surface has action wheel (3), No. two frame (2) internal rotation installs two and follows driving wheel (4), be fixed with driving motor (5) on the frame (1) lateral wall, swing joint has chain (6) between the output axle head of driving motor (5) and the pivot, chain (6) are used for driving action wheel (3) to rotate, No. two frame (2) lateral wall is fixed with gets water tank (7), the top and the bottom of getting water tank (7) all are equipped with the through-hole, it is equipped with straight-bar (8) to get water tank (7) internalization, straight-bar (8) and through-hole are clearance fit, straight-bar (8) top is equipped with buoyancy ball (9), straight-bar (8) bottom is equipped with filter screen (10), get water tank (7) bottom and be equipped with filtration grid tray (11), be equipped with waterproof stopper (12) on getting water tank (7) lateral wall, be equipped with battery and controller on No. two frame (2), battery and controller are electric connection, and the controller is used for controlling sampling device work.
2. The method for rapidly detecting the microorganisms in the environmental water body according to claim 1, which is characterized in that: frame (1) bottom is rotated and is installed two disks (13), No. two frames (2) bottom is rotated and is installed two disks (14), a disk (13) and adjacent two disks (14) closely laminate, the output shaft end of driving motor (5) is fixed with drive wheel (15), drive wheel (15) and one of them disk (13) closely laminate, two between a disk (13), two all be fixed with blade (16) more than two along respective circumference equidistance between two disks (14), blade (16) are the arc structure.
3. The method for rapidly detecting the microorganisms in the environmental water body according to claim 2, which is characterized in that: a cylinder (17) is fixed between the two first disks (13), cylinders (17) are fixed on the two second disks (14), and rectangular plates (18) are hinged on the surfaces of the two cylinders (17) at equal intervals along the circumferential directions of the two cylinders (17).
4. The method for rapidly detecting the microorganisms in the environmental water body according to claim 3, which is characterized in that: a frame (1) and No. two frame (2) tops all are fixed with bracing piece (19), bracing piece (19) top is equipped with spacing hole, and spacing hole department movable mounting has ring (20), ring (20) inner circle is fixed with screw (21) more than two along its circumference equidistance, frame (1) bottom rotates installs round bar (22), round bar (22) fixed surface has worm (23), worm (23) and ring (20) intermeshing, swing joint has belt (24) between the output axle head of driving motor (5) and round bar (22).
5. The method for rapidly detecting the microorganisms in the environmental water body according to claim 3, which is characterized in that: rectangular plate (18) and adjacent blade (16) staggered arrangement, be equipped with the hole of permeating water on rectangular plate (18), the one end density that adjacent drum (17) was kept away from in rectangular plate (18) is greater than the density of the other end.
6. The method for rapidly detecting the microorganisms in the environmental water body according to claim 4, wherein the method comprises the following steps: the first disc (13) is of a cavity structure, and the inner cavity of the first disc (13) is filled with water-absorbent resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010576352.7A CN111896315A (en) | 2020-06-22 | 2020-06-22 | Rapid detection method for environmental water microorganisms |
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| CN202010576352.7A CN111896315A (en) | 2020-06-22 | 2020-06-22 | Rapid detection method for environmental water microorganisms |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113405848A (en) * | 2021-06-16 | 2021-09-17 | 枣庄科技职业学院 | Aquatic pathogenic microorganism collection device |
| CN113447318A (en) * | 2021-08-19 | 2021-09-28 | 潍坊水发供水集团有限公司 | Portable water body sampler and use method thereof |
-
2020
- 2020-06-22 CN CN202010576352.7A patent/CN111896315A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113405848A (en) * | 2021-06-16 | 2021-09-17 | 枣庄科技职业学院 | Aquatic pathogenic microorganism collection device |
| CN113447318A (en) * | 2021-08-19 | 2021-09-28 | 潍坊水发供水集团有限公司 | Portable water body sampler and use method thereof |
| CN113447318B (en) * | 2021-08-19 | 2022-08-19 | 潍坊水发供水集团有限公司 | Portable water body sampler and use method thereof |
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Application publication date: 20201106 |