CN113395595B - Intelligent two-dimensional video playing method related to density and video device thereof - Google Patents
Intelligent two-dimensional video playing method related to density and video device thereof Download PDFInfo
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N21/00—Selective content distribution, e.g. interactive television or video on demand [VOD]
- H04N21/40—Client devices specifically adapted for the reception of or interaction with content, e.g. set-top-box [STB]; Operations thereof
- H04N21/47—End-user applications
- H04N21/472—End-user interface for requesting content, additional data or services; End-user interface for interacting with content, e.g. for content reservation or setting reminders, for requesting event notification, for manipulating displayed content
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N21/00—Selective content distribution, e.g. interactive television or video on demand [VOD]
- H04N21/40—Client devices specifically adapted for the reception of or interaction with content, e.g. set-top-box [STB]; Operations thereof
- H04N21/43—Processing of content or additional data, e.g. demultiplexing additional data from a digital video stream; Elementary client operations, e.g. monitoring of home network or synchronising decoder's clock; Client middleware
- H04N21/431—Generation of visual interfaces for content selection or interaction; Content or additional data rendering
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N21/00—Selective content distribution, e.g. interactive television or video on demand [VOD]
- H04N21/40—Client devices specifically adapted for the reception of or interaction with content, e.g. set-top-box [STB]; Operations thereof
- H04N21/43—Processing of content or additional data, e.g. demultiplexing additional data from a digital video stream; Elementary client operations, e.g. monitoring of home network or synchronising decoder's clock; Client middleware
- H04N21/44—Processing of video elementary streams, e.g. splicing a video clip retrieved from local storage with an incoming video stream or rendering scenes according to encoded video stream scene graphs
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Abstract
The invention discloses a density-related intelligent two-dimensional video playing method and an intelligent two-dimensional video device thereof. The method comprises the following steps: connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different densities of an ultrathin section sample, a plurality of substance molecules with different densities are distributed on the ultrathin section sample, and the plurality of images are sequenced from small to large according to the densities; and playing the two-dimensional video to enable an operator to quickly view the plurality of images respectively corresponding to the plurality of distributions with different densities in the two-dimensional video. The invention at least comprises the following advantages: the density of each substance molecule in the ultrathin section sample can be rapidly detected, and the excessive quantity of which substance molecule can be known by matching with fluorescent molecular markers with different colors, so that doctors can be assisted to rapidly judge whether a patient is abnormal or not.
Description
Technical Field
The invention relates to the technical field of picture imaging, in particular to a density-related intelligent two-dimensional video playing method based on a microscope-shot picture and a density-related intelligent two-dimensional video device thereof.
Background
The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.
Microscopes project an accelerated and focused beam of electrons onto a very thin sample, where the electrons collide with atoms in the sample and change direction, thereby producing solid angle scattering. The magnitude of the scattering angle is related to the density and thickness of the sample, so that images with different light and shade are formed, and the images are displayed on a fluorescent screen after being amplified and focused.
The microscope utilizes electron beams to penetrate through a thin film sample (e.g., an ultrathin slice of a human tissue or organ), which is distributed with substances of different densities, so that the electron beams penetrate to different degrees. The part with high material density, which has less penetration of electron beam, is projected into deeper zone; the portion with low material density, where the electron beam penetrates more, is projected as a shallower area. All the blocks with different depths are projected on the fluorescent plate, and the kinetic energy generated by the electron beam is converted into a two-dimensional image visible to naked eyes by the phosphoric acid substance of the fluorescent plate.
It should be noted that the above background description is only for the sake of clarity and complete description of the technical solutions of the present invention and for the understanding of those skilled in the art. These solutions are not considered to be known to the person skilled in the art merely because they are set forth in the background section of the invention.
Disclosure of Invention
In order to overcome the defects in the prior art, embodiments of the present invention provide a density-dependent intelligent two-dimensional video playing method based on a microscope-captured picture and a density-dependent intelligent two-dimensional video apparatus thereof, where the intelligent two-dimensional video apparatus can rapidly switch between different densities to view the texture and the overall view of different regions of an object under study.
The embodiment of the application discloses: a density-related intelligent two-dimensional video playing method comprises the following steps: connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of ultrathin slice samples with different densities, and the plurality of images are sequenced from small to large according to the densities; and playing the two-dimensional video to allow an operator to quickly view the plurality of images respectively corresponding to the plurality of distributions with different densities in the two-dimensional video.
Further, the method further comprises: and identifying the two-dimensional video according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample respectively correspond to the plurality of fluorescent molecular markers with different colors.
Further, the method further comprises: calculating the molecular density of the plurality of different substance molecules for each image; and displaying the molecular density of the plurality of different substance molecules in the marked two-dimensional video.
Further, the method further comprises: the marked two-dimensional video is identified according to a specified density range to generate a low-risk section and a high-risk section.
Further, the method further comprises: playing the low-risk section of the marked two-dimensional video at a first playing speed, and playing the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
The embodiment of the application discloses: a density-dependent intelligent two-dimensional video device comprising: the system comprises a video generating unit, a video generating unit and a video processing unit, wherein the video generating unit is used for connecting a plurality of images generated by a microscope imaging system in series into a two-dimensional video, the plurality of images respectively correspond to a plurality of distributions with different densities of an ultrathin slice sample, a plurality of substance molecules with different densities are distributed on the ultrathin slice sample, and the plurality of images are sequenced from small to large according to the densities; and a display unit, coupled to the video generation unit, for playing the two-dimensional video to enable an operator to quickly view the plurality of images respectively corresponding to the plurality of distributions with different densities in the two-dimensional video.
Furthermore, the density-related intelligent two-dimensional video device further comprises a marking unit, coupled to the video generating unit, for identifying the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample correspond to the plurality of fluorescent molecular markers of different colors, respectively.
Further, the density-dependent intelligent two-dimensional video apparatus further comprises a calculating unit, coupled to the marking unit, for calculating the molecular density of the plurality of different substance molecules for each image. The display unit displays the molecular density of the plurality of different substance molecules in the marked two-dimensional video.
Further, the density-dependent intelligent two-dimensional video apparatus further comprises an intelligent identification unit, coupled to the computing unit, for identifying the marked two-dimensional video according to a specified density range to generate a low-risk section and a high-risk section.
Furthermore, the density-dependent intelligent two-dimensional video apparatus further comprises an intelligent play control unit, coupled to the intelligent recognition unit and the display unit, for controlling the display unit to play the low-risk segment of the marked two-dimensional video at a first play speed and to play the high-risk segment of the marked two-dimensional video at a second play speed, wherein the first play speed is greater than the second play speed.
By means of the technical scheme, the invention has the following beneficial effects: images shot under a microscope are displayed on a screen after being spliced, and a two-dimensional video is generated, so that the overall appearance of a researched object under a microscopic field of view can be conveniently checked, and an interested area can be conveniently researched. In addition, the invention provides a density-related intelligent two-dimensional video playing method and a density-related intelligent two-dimensional video device thereof, which can automatically adjust the playing speed according to the content of the video, and play the low-risk section at a higher playing speed when detecting that the video is a low-risk section; when the two-dimensional video is detected to be a high-risk section corresponding to the designated density range, the high-risk section is played at a slower playing speed. The invention can quickly detect whether the density and the density distribution of each substance molecule in the ultrathin slice sample are uniform, and can know which substance molecules are excessive by matching with fluorescent molecular markers with different colors, thereby assisting a doctor to quickly judge whether a patient is abnormal.
In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flowchart of a density-dependent intelligent two-dimensional video playing method according to a first embodiment of the present invention.
Fig. 2 is a flowchart of a density-dependent intelligent two-dimensional video playing method according to a second embodiment of the present invention.
FIG. 3 is a block diagram of a density-dependent intelligent two-dimensional video apparatus according to a first embodiment of the present invention.
FIG. 4 is a block diagram of a density-dependent intelligent two-dimensional video apparatus according to a second embodiment of the present invention.
Reference numerals of the above figures: s110, S120, S210, S220, S230, S240, S250 and a step; 30. 40, a density-dependent intelligent two-dimensional video device; 310. a video generation unit; 320. 420, a display unit; 430. a marking unit; 440. a calculation unit; 450. an intelligent identification unit; 460. and a smart play control unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, in the description of the present invention, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is considered as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
It can be seen by microscopy that the appearance of tumor cells and normal cells is quite different. Normal cells are substantially uniform in size, have a directional arrangement, and appear to be small in size. The size of the tumor cells is very different, the cell nucleus is irregular in shape, grows densely, and is arranged in a disordered way, and the cell nucleus of the tumor cells is very dark because the tumor cells contain excessive chromosomes (DNA).
Referring to fig. 1, fig. 1 is a flowchart illustrating a density-dependent intelligent two-dimensional video playing method according to a first embodiment of the present invention. The method comprises the following steps:
s110: the method comprises the steps of connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different densities of an ultrathin section sample, a plurality of substance molecules with different densities are distributed on the ultrathin section sample, and the plurality of images are sequenced from small to large according to the densities.
S120: and playing the two-dimensional video to enable an operator to quickly view a plurality of images which respectively correspond to a plurality of distributions with different densities in the two-dimensional video.
In one possible embodiment, the ultrathin section sample can be a human tissue organ section. For example, a first ultrathin section sample includes only normal cells, and a second ultrathin section sample includes both normal cells and tumor cells. Firstly, a plurality of images generated after a microscope imaging system shoots human tissue and organ slices are connected in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of ultrathin slice samples with different densities, a plurality of substance molecules with different densities are distributed on the ultrathin slice samples, and the plurality of images are sorted from small to large according to the densities. Since the first ultrathin section sample includes only normal cells, which are substantially uniform in size and have small nuclei, the first ultrathin section sample has a small density and a uniform density distribution, and the nuclei appear bright. The second ultrathin section sample simultaneously comprises normal cells and tumor cells, the size difference of the tumor cells is large, the growth of the tumor cells is dense, the density of the second ultrathin section sample is high, the density distribution is uneven, and the cell nucleus looks dark. Therefore, when the two-dimensional video is played, an operator can watch the plurality of images respectively corresponding to the plurality of distributions with different densities in the two-dimensional video too quickly, and further know whether the tissue cells of the patient are normal or not through the density size and the density distribution of the ultrathin section sample.
Referring to fig. 2, fig. 2 is a flowchart illustrating a density-dependent intelligent two-dimensional video playing method according to a second embodiment of the present invention. The method comprises the following steps:
s210: the method comprises the steps of connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different densities of an ultrathin section sample, a plurality of substance molecules with different densities are distributed on the ultrathin section sample, and the plurality of images are sequenced from small to large according to the densities.
S220: and identifying the two-dimensional video according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample respectively correspond to the plurality of fluorescent molecular markers with different colors.
S230: calculating the molecular density of the plurality of different substance molecules for each image; and displaying the molecular density of the plurality of different substance molecules in the marked two-dimensional video.
S240: the marked two-dimensional video is identified according to a designated density range to generate a low-risk section and a high-risk section.
S250: playing the low-risk section of the marked two-dimensional video at a first playing speed, and playing the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
For example, the three component molecules of tumor cells can be stained with different fluorescent substances, DNA is stained blue, INCENP protein is stained green, and microtubules are stained purple, so that a plurality of images generated by the microscope imaging system will be overlaid to give a labeled two-dimensional video with fluorescent molecular labels of different colors, and the DNA stained blue, the INCENP protein stained green, and the microtubules stained purple can be clearly distinguished.
Referring to fig. 4, in step S220, the labeling unit 430 identifies the two-dimensional video according to the blue, green and purple fluorescent molecular markers to generate a labeled two-dimensional video, wherein the labeled two-dimensional video includes the blue, green and purple fluorescent molecular markers, which correspond to the blue-stained DNA, the green-stained INCENP protein and the purple-stained microtubule molecules in the collected liquid sample, respectively.
In step S230, the molecular densities of DNA, INCENP protein and microtubules may be further separated and displayed in the labeled two-dimensional video. For example, the first microtomed sample has a lower density and a more uniform density distribution, the nuclei appear brighter, the incinp protein density is lower, and the DNA molecule density of the nuclei is lower. The second microtomed sample had a higher density of INCENP protein and an abnormally high density of nuclear DNA molecules. At this time, the doctor can easily determine which substance molecule has an abnormal number according to the molecular density of the substance molecules and whether the density distribution is uniform.
In one possible embodiment, the playing speed of the two-dimensional video can be automatically adjusted. In step S250, when it is detected that the two-dimensional video is a low-risk section, the low-risk section is played at a faster playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to a specified density range (for example, the density is higher than a specific threshold), the high-risk section is played at a slower playing speed, so as to assist a doctor to quickly judge whether the patient has an abnormality.
For example, since the second ultrathin section sample includes tumor cells and normal cells, the second ultrathin section sample has a higher density of the INCENP protein and an abnormally high density of DNA molecules in the nucleus. Therefore, the invention can rapidly detect whether the molecular density of each substance molecule in the ultrathin section sample is uniform or not and the density distribution is uniform, and can know which substance molecule is excessive by matching with fluorescent molecular markers with different colors, thereby assisting doctors to rapidly judge whether a patient is abnormal or not.
Referring to fig. 3, fig. 3 is a block diagram of a density-dependent intelligent two-dimensional video apparatus 30 according to a first embodiment of the present invention. The density-dependent intelligent two-dimensional video apparatus 30 includes a video generation unit 310 and a display unit 320. The video generating unit 310 is configured to concatenate a plurality of images generated by a microscope imaging system into a two-dimensional video, wherein the plurality of images respectively correspond to a plurality of distributions of different densities of an ultrathin section sample, and the plurality of images are sorted according to the densities from small to large. The display unit 320 is coupled to the video generating unit 310 for playing the two-dimensional video for an operator to quickly view the plurality of images corresponding to the plurality of distributions with different densities in the two-dimensional video.
Referring to fig. 4, fig. 4 is a block diagram of a density-dependent intelligent two-dimensional video apparatus 40 according to a second embodiment of the present invention. In the present embodiment, the density-dependent intelligent two-dimensional video apparatus 40 includes a video generating unit 310, a display unit 420, a marking unit 430, a calculating unit 440, an intelligent identification unit 450, and an intelligent playback control unit 460. The marking unit 430 is coupled to the video generating unit 310, and configured to identify the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample correspond to the plurality of fluorescent molecular markers of different colors, respectively. The calculating unit 440, coupled to the labeling unit 430, calculates the molecular density of the plurality of different molecules for each image; at this time, the display unit 420 displays the molecular density of the plurality of different substance molecules in the labeled two-dimensional video. The intelligent recognition unit 450 is coupled to the calculation unit 440 and is configured to recognize the marked two-dimensional video according to a specified density range to generate a low risk section and a high risk section. The smart play control unit 460 is coupled to the smart identification unit 450 and the display unit 420, and configured to control the display unit 420 to play the low risk segment of the two-dimensional video at a first play speed and play the high risk segment of the two-dimensional video at a second play speed, where the first play speed is greater than the second play speed.
In other words, the density-dependent intelligent two-dimensional video device 40 is capable of automatically adjusting the playback speed of the two-dimensional video. When the two-dimensional video is detected to be a low-risk section, the low-risk section is played at a faster playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to a specified density range (for example, the density is higher than a specific threshold), the high-risk section is played at a slower playing speed, so as to assist a doctor to quickly judge whether the patient has an abnormality.
By means of the technical scheme, the invention has the following beneficial effects: images shot under a microscope are spliced and then displayed on a screen, a two-dimensional video is generated, the overall appearance of a researched object in a microscopic view can be conveniently checked, and an interested area can be conveniently researched. In addition, the invention provides a density-related intelligent two-dimensional video playing method and a density-related intelligent two-dimensional video device thereof, which can automatically adjust the whole playing speed of a two-dimensional video, and when the video is detected to be a low-risk section, the low-risk section can be played at a higher playing speed; when the two-dimensional video is detected to be a high-risk section corresponding to the designated density range, the high-risk section is played at a slower playing speed. The density of each substance molecule in the ultrathin section sample can be rapidly detected through the invention, and the excessive number of the substance molecules can be known by matching with fluorescent molecular markers with different colors, so that doctors can be assisted to rapidly judge whether a patient is abnormal.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (4)
1. A density-related intelligent two-dimensional video playing method is characterized by comprising the following steps:
connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different densities of an ultrathin section sample, a plurality of substance molecules with different densities are distributed on the ultrathin section sample, and the plurality of images are sequenced from small to large according to the densities;
playing the two-dimensional video to enable an operator to quickly view the plurality of images which respectively correspond to the plurality of distributions with different densities in the two-dimensional video;
identifying the two-dimensional video according to a plurality of fluorescent molecular markers with different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample respectively correspond to the plurality of fluorescent molecular markers with different colors;
calculating the molecular density of the plurality of different substance molecules for each image;
displaying the molecular densities of the plurality of different substance molecules in the labeled two-dimensional video;
the marked two-dimensional video is identified according to a specified density range to generate a low-risk section and a high-risk section.
2. The method of claim 1, further comprising:
playing the low-risk section of the marked two-dimensional video at a first playing speed, and playing the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
3. A density-dependent intelligent two-dimensional video apparatus, comprising:
the video generation unit is used for connecting a plurality of images generated by a microscope imaging system in series to form a two-dimensional video, wherein the plurality of images respectively correspond to the distribution of a plurality of different densities of an ultrathin section sample, a plurality of substance molecules with different densities are distributed on the ultrathin section sample, and the plurality of images are sequenced from small to large according to the densities;
a display unit, coupled to the video generating unit, for displaying the two-dimensional video for an operator to quickly view the plurality of images respectively corresponding to the plurality of distributions with different densities in the two-dimensional video;
a marking unit, coupled to the video generating unit, for identifying the two-dimensional video according to a plurality of fluorescent molecular markers of different colors to generate a marked two-dimensional video, wherein the plurality of different substance molecules on the ultrathin section sample correspond to the plurality of fluorescent molecular markers of different colors, respectively;
a calculating unit, coupled to the marking unit, for calculating the molecular density of the plurality of different material molecules for each image; and
an intelligent identification unit, coupled to the computing unit, for identifying the marked two-dimensional video according to a specified density range to generate a low risk section and a high risk section;
wherein the display unit displays the molecular density of the plurality of different substance molecules in the labeled two-dimensional video.
4. The intelligent density-dependent two-dimensional video apparatus according to claim 3, further comprising:
and the intelligent playing control unit is coupled with the intelligent identification unit and the display unit and used for controlling the display unit to play the low-risk section of the marked two-dimensional video at a first playing speed and play the high-risk section of the marked two-dimensional video at a second playing speed, wherein the first playing speed is greater than the second playing speed.
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