CN113288941A - Composition containing raspberry exosome for maintaining ovary and preparation method thereof - Google Patents

Composition containing raspberry exosome for maintaining ovary and preparation method thereof Download PDF

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Publication number
CN113288941A
CN113288941A CN202110573391.6A CN202110573391A CN113288941A CN 113288941 A CN113288941 A CN 113288941A CN 202110573391 A CN202110573391 A CN 202110573391A CN 113288941 A CN113288941 A CN 113288941A
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raspberry
exosomes
exosome
powder
composition
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CN202110573391.6A
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吴丹丹
杨琼
靳莉
李珍珍
曹美霞
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Dongguan Natural Salutem Health Technology Co ltd
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Dongguan Natural Salutem Health Technology Co ltd
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
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    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01—Instant products; Powders; Flakes; Granules
    • A—HUMAN NECESSITIES
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    • A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
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Abstract

The invention relates to the field of biomedicine. Specifically, the invention relates to a raspberry exosome-containing composition for maintaining ovaries and a preparation method thereof. The invention provides an ovary care composition containing raspberry exosomes, which comprises raspberry exosomes, bitter gourd exosomes, spina date seed exosomes, functional raw materials and auxiliary materials. The raspberry exosome-containing ovarian maintenance composition provided by the invention has the effects of maintaining ovaries, improving ovarian functions and improving endocrine levels.

Description

Composition containing raspberry exosome for maintaining ovary and preparation method thereof
Technical Field
The invention relates to the field of biomedicine. Specifically, the invention relates to a raspberry exosome-containing composition for maintaining ovaries and a preparation method thereof.
Background
The ovary is one of the important endocrine glands of women, and its main functions are to secrete female hormones and to produce ova. In recent years, with the increasing social pressure of women, the incidence of premature ovarian failure is increasing year by year due to the influence of various factors such as psychology, sleep, bad life style and environmental pollution. Female infertility is often related to ovaries, and once the ovaries are premature or hypofunction, the premature ovarian failure or hypofunction can cause rapid aging of all parts of the human body. Ovarian hypofunction has a process of onset and progression, where hormone levels may be normal, and Follicle Stimulating Hormone (FSH) may also be normal initially, and then gradually become abnormal. Ovarian diseases are mainly manifested in menstrual periods, and are often manifested as irregular menstruation, which has many different manifestations, such as advanced menstrual period, delayed menstrual period, excessive menstrual blood, scanty menstrual blood, irregular menstrual period, etc.
However, ovarian dysfunction is not irreversible, as long as it is not debilitating, and clinical symptoms can be significantly ameliorated by active treatment. At present, premature ovarian failure is treated mainly by hormone therapy, and patients often need small amount of estrogen or female and progestogen treatment. However, although hormone therapy can eliminate climacteric syndrome on the surface, most people are easy to relapse after drug withdrawal, and the side effects of hormone also bring great burden to the mind and body of patients. In addition, the medicine can regulate endocrine to treat, thereby achieving the effect of improving symptoms. However, the herbs are three-way toxic, especially some Chinese herbs, which can cause heavy load of viscera and affect metabolism of body after long-term administration.
The traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition is capable of supplementing medicinal and edible traditional Chinese medicine components for nourishing yin, tonifying kidney, enriching blood, tonifying yang, soothing liver, strengthening spleen and stomach, can effectively protect and improve premature ovarian failure of women, improve the function decline of ovarian stores, promote ovarian hyporeaction and the like, adopts a plurality of beneficial components for nourishing liver and kidney, tonifying kidney, replenishing essence, nourishing yin, moistening lung, nourishing qi, enriching blood, promoting the production of body fluid, quenching thirst and the like, plays a role in increasing ovarian reactivity, protecting and improving ovarian function and promoting follicular development, and can benefit people with decreased ovarian stores, premature ovarian failure and the like.
Therefore, an innovative medicinal and edible composition is developed and applied to products for preventing and improving ovarian diseases, and the medicinal and edible composition has important significance and wide market prospect.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides an ovary care composition containing raspberry exosomes.
The purpose of the invention is realized by the following technical scheme:
in a first aspect, the invention provides an ovary care composition containing raspberry exosomes, which comprises raspberry exosomes, bitter gourd exosomes, spina date seed exosomes, functional raw materials and auxiliary materials.
In a preferred embodiment, the raspberry exosomes are raspberry exosome lyophilized powders.
In a preferred embodiment, the bitter gourd exosomes are bitter gourd exosome lyophilized powders.
In a preferred embodiment, the spine date seed exosomes are spine date seed exosome freeze-dried powders.
In a preferred embodiment, the functional raw material is selected from one or more of hawthorn powder, lotus leaf powder, black fungus powder, wax gourd seed powder and soybean protein powder.
In a preferred embodiment, the excipient is selected from one or more of xylitol, lactose, D-mannitol, magnesium stearate, film coating agents and maltodextrin.
In a second aspect, there is provided a raspberry exosome containing ovarian maintenance product comprising a composition according to the invention.
In a third aspect, use of raspberry exosomes in ovarian maintenance is provided.
In a preferred embodiment, the raspberry exosomes are raspberry exosome lyophilized powders.
In a fourth aspect, there is provided the use of a composition comprising raspberry exosomes for ovarian maintenance.
In a preferred embodiment, the raspberry exosomes are raspberry exosome lyophilized powders.
In a fifth aspect, there is provided use of raspberry exosomes in the preparation of an ovarian maintenance product.
In a preferred embodiment, the raspberry exosomes are raspberry exosome lyophilized powders.
In a sixth aspect, there is provided the use of a composition comprising raspberry exosomes in the preparation of an ovarian maintenance product.
In a preferred embodiment, the raspberry exosomes are raspberry exosome lyophilized powders.
In a preferred embodiment, the raspberry exosome freeze-dried powder is 0.1-5 parts by weight.
In a seventh aspect, there is provided a method of preparing a raspberry exosome containing ovarian maintenance composition, the method comprising the steps of:
s1, adding raspberry exosomes, bitter gourd exosomes, spina date seed exosomes, functional raw materials and auxiliary materials into a mixer, and mixing for 10-20min to obtain a raw material mixture;
s2, adding 85-95% ethanol into the raw material mixture, mixing, and then sieving with a 10-30-mesh sieve to obtain wet particles;
s3, placing the wet granules in a drying oven at 40-80 ℃ for 1-5h to obtain dry granules, and then sieving the dry granules by a sieve of 10-20 meshes to obtain integral granules;
s4, adding magnesium stearate into the whole granules, and totally mixing for 25-35 min.
In an eighth aspect, there is provided a method of preparing a further composition for ovarian maintenance comprising raspberry exosomes, the method comprising the steps of:
s1, adding raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder, spina date seed exosome freeze-dried powder, functional raw materials and auxiliary materials into a mixer to mix for 10-20min to obtain a raw material mixture;
s2, adding 85-95% ethanol into the raw material mixture, mixing, and then sieving with a 10-30-mesh sieve to obtain wet particles;
s3, placing the wet granules in a drying oven at 40-80 ℃ for 1-5h to obtain dry granules, and then sieving the dry granules by a sieve of 10-20 meshes to obtain integral granules;
s4, adding magnesium stearate into the whole granules, and totally mixing for 25-35 min.
In a ninth aspect, the invention provides a composition for maintaining ovaries and containing raspberry exosomes, which comprises raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, a film coating agent and maltodextrin.
In a preferred embodiment, the film coating agent is selected from polyvinyl alcohol and/or beeswax.
In a tenth aspect, the present invention provides a method for preparing a lyophilized powder of plant exosomes, the method comprising the steps of:
s1, adding 200-250mL sterilized PBS into 200-250g fresh plants, and crushing for 15min by using a crusher to obtain plant homogenate;
s2, centrifuging the plant homogenate obtained in the step S1 at 4 ℃ and 4500 Xg for 20min, and removing large residues, wherein the solution is divided into three layers, the upper layer is light residues, the middle layer is transparent solution with various plant colors, and the lower layer is a small amount of precipitate;
s3, centrifuging the middle layer liquid obtained in the step S2 at 4 ℃ at 10000 Xg for 60min, and reserving the upper layer liquid to obtain a plant exosome extracting solution;
s4, compounding the plant exosome extracting solution obtained in the step S3 with mannitol to form a uniform solution, injecting the solution into a mold, pre-freezing for 0.5-3h, transferring into a freeze dryer, and freeze-drying for 5-10h under the conditions of 0.01-10mbar and-50-4 ℃ to obtain the plant exosome freeze-dried powder.
In a preferred embodiment, the plant exosome freeze-dried powder is raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder or spina date seed exosome freeze-dried powder.
According to an eleventh aspect, the invention provides a composition for maintaining an ovary, which comprises, by weight, 0.1-5 parts of raspberry exosome freeze-dried powder, 2-10 parts of bitter gourd exosome freeze-dried powder, 5-10 parts of spina date seed exosome freeze-dried powder, 2-10 parts of hawthorn powder, 0.5-5 parts of lotus leaf powder, 0.5-5 parts of black fungus powder, 0.5-5 parts of white gourd seed powder, 2-10 parts of soybean protein powder, 2-10 parts of xylitol, 3-10 parts of lactose, 1-10 parts of D-mannitol, 0.5-2 parts of magnesium stearate, 0.5-2 parts of polyvinyl alcohol and 5-20 parts of maltodextrin.
In a twelfth aspect, the invention provides a composition for maintaining an ovary, which comprises, by weight, 3 parts of raspberry exosome freeze-dried powder, 5 parts of bitter gourd exosome freeze-dried powder, 7 parts of spina date seed exosome freeze-dried powder, 5 parts of hawthorn powder, 3 parts of lotus leaf powder, 3 parts of black fungus powder, 3 parts of white gourd seed powder, 5 parts of soybean protein powder, 8 parts of xylitol, 8 parts of lactose, 7 parts of D-mannitol, 1 part of magnesium stearate, 1 part of polyvinyl alcohol and 12 parts of maltodextrin.
The taking method of the raspberry exosome-containing ovarian maintenance composition provided by the invention comprises the following steps: it is administered half an hour after meal for 1-2 times a day, 2 tablets each time.
In the present invention, raspberry exosomes: the raspberry is warm in nature, sweet in taste and acidic, has activities of resisting oxidation, inflammation, thrombus and tumor, and mainly contains compounds such as flavonoid, terpenoids, steroids and phenolic acids. The raspberry has the function of resisting osteoporosis and is related to the estrogen-like activity of chemical components of the raspberry, and researches show that the ethyl acetate part of the raspberry has stronger estrogen-like effect and obviously promotes the MCF-7 cell proliferation. One of the main active ingredients in the raspberry is a flavonoid compound, belongs to phytoestrogens, and is an important natural organic compound produced in the metabolic process of the raspberry. Sweet and sweet can tonify, sour and astringent, warm in nature without dryness-heat, tonify kidney yang without damage to yin, relieve depletion without stagnation, tonify and consolidate, benefit lower-jiao cang, it is good at treating insecurity of kidney deficiency. In addition, raspberry fruits contain quite abundant nutrient elements such as vitamin a, vitamin C, calcium, potassium, magnesium and the like, and a large amount of fibers.
Bitter gourd exosomes: balsam pear, a plant of the genus Momordica of the family Cucurbitaceae, is sweet, bitter, cold and cool in nature, and has the effects of clearing heat, relieving restlessness and quenching thirst. The balsam pear can enhance the skin vitality, enables the skin to become fine and healthy, and has the functions of beautifying and skin tendering; the crude extract of balsam pear contains similar insulin substances and has obvious effect of reducing blood sugar; is rich in vitamin C, is an ultra-strong antioxidant, prevents scurvy, prevents atherosclerosis and protects heart; reducing fat accumulation, moistening dry skin, whitening skin, and keeping moisture.
Wild jujube seed exosome: suan Zao ren, listed as the superior product in Ben Cao gang mu. Sweet, sour and neutral. It enters liver, gallbladder and heart meridians, and has effects of nourishing heart, tonifying liver, calming heart, tranquilizing mind, arresting sweating, and promoting fluid production. Can be used for treating vexation, insomnia, palpitation, dreaminess, asthenia, hyperhidrosis, body fluid deficiency, and thirst. Modern researches have shown that the spina date seed has the effects of tranquilizing and hypnotizing, relieving pain, resisting convulsion, reducing blood fat, resisting platelet aggregation, enhancing immunity, reducing blood pressure, resisting anoxia, resisting myocardial ischemia, resisting arrhythmia, resisting aging, resisting radiation and the like.
Hawthorn powder: the hawthorn is a tree species with special Chinese medicinal and fruit functions, mainly contains components such as maslinic acid, citric acid, lipolysis acid, vitamin C, flavone, carbohydrate and the like, has the effects of expanding blood vessels, improving microcirculation, reducing blood fat, reducing blood pressure, strengthening heart, resisting arrhythmia and the like, is also a good medicine for strengthening spleen, stimulating appetite, promoting digestion, removing food stagnation, promoting blood circulation and reducing phlegm, and has good curative effects on symptoms such as chest, diaphragm, spleen fullness, hernia, blood stasis, amenorrhea and the like. The vitexin is a flavonoid compound in hawthorn, is a drug with strong anticancer effect, and the extract of the vitexin has certain effects on inhibiting the growth, proliferation and infiltration and metastasis of cancer cells in vivo.
Lotus leaf powder: bitter and even, entering liver, spleen and stomach meridians. Clear summer-heat, resolve dampness, raise hair, clear yang, cool blood and stop bleeding. Can be used for treating summer heat, polydipsia, summer damp diarrhea, spleen deficiency diarrhea, blood heat, expectoration, hematochezia, metrorrhagia, and metrostaxis. The folium Nelumbinis is rich in folium Nelumbinis flavone, and has effects of reducing weight and blood lipid, resisting oxidation, relieving convulsion, and protecting liver.
Black fungus powder: the black fungus is sweet in nature and taste, and has the effects of clearing lung, moistening intestines, nourishing yin, enriching blood, promoting blood circulation to remove blood stasis, improving eyesight, nourishing stomach and the like. Can be used for treating metrorrhagia, hemorrhoid, bloody dysentery, anemia, constipation, etc. Meanwhile, the hair ferment and the plant alkali contained in the medicine can promote the secretion of the digestive tract and the urinary tract, and coordinate with the secretion to catalyze the calculus, thereby having obvious decomposition effect on gallstone, kidney stone and the like. Regarding modern medicinal research of black fungus, the black fungus is firstly found to have remarkable effect of inhibiting platelet aggregation, thereby attracting the attention of people. It can be used for treating female leukorrhagia, blood clotting in abdomen, and swelling and pain, and also for treating epistaxis, and intestinal wind and blood diarrhea, benefiting five viscera, and benefiting gastrointestinal qi and expelling toxic gas. The dried Auricularia auricula has high contents of protein, vitamins and ferrum, and the protein contains various amino acids, especially lysine and leucine.
White gourd seed powder: the wax gourd seeds have high medicinal value, the effects of clearing lung and eliminating phlegm and no side effect. The vegetable oil contained in semen Benincasae contains linoleic acid, which is a skin-moistening cosmetic. The unsaturated fatty acid can make the appearance ruddy and glossy, the skin tender and soft, and the hair black and bright. Therefore, the wax gourd seeds have quite high cosmetic value. Modern nutrition researches find that the wax gourd seeds contain 0.68% of saponin, urea-decomposing enzyme, fatty oil, citrulline, histidine, lupulin, mannitol, beta-sitosterol and other components, and particularly contain quite rich vitamin B1. The components can effectively reduce the contents of fat and cholesterol in blood, and have positive significance for preventing and treating cardiovascular diseases.
Soybean protein powder: soybeans are rich in soybean isoflavones. The isoflavone mainly comprises daidzein, genistein, glycitein 3, and glucoside, acetyl glucoside, and malonyl glucoside. The content of isoflavones in various soybeans is 1200-4216 mu g/g, the contents of glucoside and malonyl glucoside are mainly 207-1698 mu g/g and 999-2518 mu g/g respectively. The soybean isoflavone is a phytochemical, belongs to plant flavonoid, is mainly derived from legume, and has high content of soybean of 0.1-0.5%. Since it is extracted from plants and has a structure similar to that of estrogen, soy isoflavones are also called phytoestrogens. The estrogenic action of soy isoflavones affects hormone secretion, metabolic biological activity, protein synthesis, growth factor activity, and is a natural cancer chemopreventive agent. The soybean isoflavone can make up for deficiency of hormone secretion of premature ovarian failure female, and improve ovarian function.
Xylitol: under the condition that the carbohydrate metabolism of a human body is influenced by lack of insulin in vivo, the intermediate does not need insulin promotion, xylitol can also permeate cell membranes to be absorbed and utilized by tissues, liver glycogen synthesis is promoted to provide nutrition and energy for cells, the blood sugar value cannot be increased, three or more symptoms (polyphagia, polydipsia and polyuria) after the sugar metabolism is taken by a diabetic patient are eliminated, and the xylitol is a nutritional sugar substitute which is most suitable for the diabetic patient to eat. Xylitol can also be used in weight-reducing products to provide energy for human bodies, synthesize glycogen, reduce the consumption of protein in fat and liver tissues, protect and repair livers, reduce the generation of harmful ketone bodies in human bodies, and avoid obesity and worry caused by eating the xylitol.
Lactose: in addition to supplying energy to the human body, lactose also has physiological significance different from other sugars. Lactose is not digested and absorbed in the stomach of the human body and can reach the intestinal tract directly. Lactose is easily decomposed into glucose and galactose by lactase in human intestinal tract to be absorbed. Galactose is a component of glycolipids constituting brain and nerve tissues, is important for the development of intelligence of infants, and promotes the production of cerebroside and mucopolysaccharides. Lactose can promote the generation of some lactic acid bacteria in human intestinal tract, inhibit the growth of putrefying bacteria, and promote intestinal peristalsis.
Compared with the prior art, the invention has the beneficial effects that:
the specific functional raw materials used in the raspberry exosome-containing ovarian maintenance composition provided by the invention can directly act on the ovarian function by supplementing phytoestrogen and stimulating follicular development. The blood concentration of the estrogen can promote or inhibit the release of gonadotropin, thereby indirectly influencing the ovarian function, making up the defect of insufficient hormone secretion of premature ovarian failure women and improving the ovarian function. Meanwhile, the ovary aging can be delayed by supplementing flavonoid substances and removing free radicals in the ovary so as to achieve the function of ovary maintenance, and the flavonoid also has the functions of inducing tumor cell apoptosis and resisting polycystic ovarian cyst. In addition, high content of proteins, vitamins, iron in Auricularia and high content of linoleic acid in semen Benincasae can be used for treating female metrorrhagia, leukemia, intra-abdominal agglomeration, anemia, and skin moistening.
After the specific functional raw materials used in the invention are compounded with the raspberry exosome, the balsam pear exosome and the spina date seed exosome used in the raspberry exosome-containing ovarian maintenance composition, the raspberry exosome, the balsam pear exosome and the spina date seed exosome can supplement steroid compounds (estrogen) to act on follicular development, regulate endocrine and improve ovarian function, resist bacteria and diminish inflammation to prevent female vaginal bacteria from breeding, and anthocyanin and flavonoid compounds can directly or indirectly delay ovarian senescence and improve ovarian function by scavenging free radicals and the like. In addition, it also has anti-osteoporosis effect. Specifically, the raspberry exosome has activities of oxidation resistance, inflammation resistance, thrombus resistance, tumor resistance and the like, wherein high-content estrogen is a steroid compound, has wide biological activity, plays an important role in development of female reproductive organs and maintenance of secondary sexual characteristics, and also has an important role in bone metabolism. The estrogen can directly improve follicular development and maturationImproving ovarian function; indirectly improving ovarian function, preventing or improving menstrual disorder and dysmenorrhea by regulating endocrine; the raspberry contains anthocyanin and flavonoid compounds, has strong antibacterial and anti-inflammatory capabilities, can prevent the breeding of female vaginal bacteria, has high vitamin C content, can promote the regeneration of immune cells, and enhances the autoimmune capability and disease resistance of a human body; the anthocyanin and the flavonoid compound can clear free radicals accumulated in ovaries, improve the activity of human tissue cells, inhibit pigment accumulation, play an obvious role in beautifying while delaying ovarian aging and achieve the effect of resisting aging of organisms. The ethyl acetate part of the raspberry exosome has a strong estrogen-like effect, and obviously promotes MCF-7 cell proliferation, so the raspberry exosome has an anti-osteoporosis effect. The compound bitter gourd exosome and the spina date seed exosome are compounded, high-content vitamin C in the bitter gourd exosome can improve the immunity of an organism, and the charantin can be combined with redundant acidic substances in the body to reduce the stimulation of the acidic substances to ovaries, and the compound bitter gourd exosome also has the effect of improving the activity of ovarian cells and has an important effect on the recovery of the ovaries. The high-purity jujuboside A in jujuba seed exosome not only can increase the expression of gamma-aminobutyric acid receptor, but also can influence calmodulin on Ca2+Antagonizes excitatory neurotransmitter glutamic acid in the brain, thereby influencing the brain waves and sleeping behaviors of animals, playing a role in calming and hypnotizing, indirectly regulating aging caused by body disorder through sleep improvement and playing a role in improving the functions of the body including ovary.
Meanwhile, the raspberry exosome-containing ovarian maintenance composition is convenient to carry and take, and the problem of poor medicine-food homologous taste is solved.
The preparation method of the raspberry exosome-containing ovarian maintenance composition provided by the invention is simple in process and low in energy consumption, and processes such as heating and the like which are easy to damage effective components are not involved in the preparation process, so that the effective components and the nutritional components are effectively protected; meanwhile, processes of extraction and other solvents which are easy to remain are not involved, and the method is safe and feasible.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Example 1
The embodiment provides a composition for maintaining ovaries containing raspberry exosomes, which comprises, by weight, 0.1 part of raspberry exosome freeze-dried powder, 2 parts of bitter gourd exosome freeze-dried powder, 5 parts of wild jujube seed exosome freeze-dried powder, 2 parts of hawthorn powder, 0.5 part of lotus leaf powder, 0.5 part of black fungus powder, 0.5 part of white gourd seed powder, 2 parts of soybean protein powder, 2 parts of xylitol, 3 parts of lactose, 1 part of D-mannitol, 0.5 part of magnesium stearate, 0.5 part of polyvinyl alcohol and 5 parts of maltodextrin.
This example provides a method of preparing a lyophilized powder of plant exosomes, the method comprising the steps of:
s1, adding 200mL of sterilized PBS into 200g of fresh plants, and crushing for 15min by using a crusher to obtain plant homogenate;
s2, centrifuging the plant homogenate obtained in the step S1 at 4 ℃ and 4500 Xg for 20min, and removing large residues, wherein the solution is divided into three layers, the upper layer is light residues, the middle layer is transparent solution with various plant colors, and the lower layer is a small amount of precipitate;
s3, centrifuging the middle layer liquid obtained in the step S2 at 4 ℃ at 10000 Xg for 60min, and reserving the upper layer liquid to obtain a plant exosome extracting solution;
s4, compounding the plant exosome extracting solution obtained in the step S3 with mannitol to form a uniform solution, injecting the solution into a mold, pre-freezing for 1h, transferring into a freeze dryer, and freeze-drying for 6h under the conditions of 5mbar and-30 ℃ to obtain plant exosome freeze-dried powder;
wherein the plant exosome freeze-dried powder is raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder and spina date seed exosome freeze-dried powder.
The preparation method of the raspberry exosome-containing ovarian maintenance composition comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, balsam pear exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer to mix for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Example 2
The embodiment provides a composition for maintaining ovaries containing raspberry exosomes, which comprises, by weight, 5 parts of raspberry exosome freeze-dried powder, 10 parts of bitter gourd exosome freeze-dried powder, 10 parts of wild jujube exosome freeze-dried powder, 10 parts of hawthorn powder, 5 parts of lotus leaf powder, 5 parts of black fungus powder, 5 parts of white gourd seed powder, 10 parts of soybean protein powder, 10 parts of xylitol, 10 parts of lactose, 10 parts of D-mannitol, 2 parts of magnesium stearate, 2 parts of polyvinyl alcohol and 20 parts of maltodextrin.
This example provides a method of preparing a lyophilized powder of plant exosomes, the method comprising the steps of:
s1, adding 200mL of sterilized PBS into 200g of fresh plants, and crushing for 15min by using a crusher to obtain plant homogenate;
s2, centrifuging the plant homogenate obtained in the step S1 at 4 ℃ and 4500 Xg for 20min, and removing large residues, wherein the solution is divided into three layers, the upper layer is light residues, the middle layer is transparent solution with various plant colors, and the lower layer is a small amount of precipitate;
s3, centrifuging the middle layer liquid obtained in the step S2 at 4 ℃ at 10000 Xg for 60min, and reserving the upper layer liquid to obtain a plant exosome extracting solution;
s4, compounding the plant exosome extracting solution obtained in the step S3 with mannitol to form a uniform solution, injecting the solution into a mold, pre-freezing for 1h, transferring into a freeze dryer, and freeze-drying for 6h under the conditions of 5mbar and-30 ℃ to obtain plant exosome freeze-dried powder;
wherein the plant exosome freeze-dried powder is raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder and spina date seed exosome freeze-dried powder.
The preparation method of the raspberry exosome-containing ovarian maintenance composition comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, balsam pear exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer to mix for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Example 3
The embodiment provides a composition for maintaining ovaries containing raspberry exosomes, which comprises 3 parts of raspberry exosome freeze-dried powder, 5 parts of bitter gourd exosome freeze-dried powder, 7 parts of wild jujube exosome freeze-dried powder, 5 parts of hawthorn powder, 3 parts of lotus leaf powder, 3 parts of black fungus powder, 3 parts of white gourd seed powder, 5 parts of soybean protein powder, 8 parts of xylitol, 8 parts of lactose, 7 parts of D-mannitol, 1 part of magnesium stearate, 1 part of polyvinyl alcohol and 12 parts of maltodextrin in parts by weight.
This example provides a method of preparing a lyophilized powder of plant exosomes, the method comprising the steps of:
s1, adding 200mL of sterilized PBS into 200g of fresh plants, and crushing for 15min by using a crusher to obtain plant homogenate;
s2, centrifuging the plant homogenate obtained in the step S1 at 4 ℃ and 4500 Xg for 20min, and removing large residues, wherein the solution is divided into three layers, the upper layer is light residues, the middle layer is transparent solution with various plant colors, and the lower layer is a small amount of precipitate;
s3, centrifuging the middle layer liquid obtained in the step S2 at 4 ℃ at 10000 Xg for 60min, and reserving the upper layer liquid to obtain a plant exosome extracting solution;
s4, compounding the plant exosome extracting solution obtained in the step S3 with mannitol to form a uniform solution, injecting the solution into a mold, pre-freezing for 1h, transferring into a freeze dryer, and freeze-drying for 6h under the conditions of 5mbar and-30 ℃ to obtain plant exosome freeze-dried powder;
wherein the plant exosome freeze-dried powder is raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder and spina date seed exosome freeze-dried powder.
The preparation method of the raspberry exosome-containing ovarian maintenance composition comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, balsam pear exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer to mix for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Comparative example 1
To further illustrate the beneficial effects of the present invention, comparative example 1 is provided, which is different from example 3 in that: the balsam pear exosome freeze-dried powder is not added.
The preparation method of the composition for maintaining the ovary containing raspberry exosomes of the comparative example comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer, and mixing for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Comparative example 2
To further illustrate the beneficial effects of the present invention, comparative example 2 is provided, which comparative example 2 differs from example 3 in that: semen Ziziphi Spinosae exosome lyophilized powder is not added.
The preparation method of the composition for maintaining the ovary containing raspberry exosomes of the comparative example comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, soybean protein powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer to mix for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Comparative example 3
To further illustrate the beneficial effects of the present invention, comparative example 3 is provided, which comparative example 3 differs from example 3 in that: no soy protein powder was added.
The preparation method of the composition for maintaining the ovary containing raspberry exosomes of the comparative example comprises the following steps:
s1, adding raspberry exosome freeze-dried powder, bitter gourd exosome freeze-dried powder, spina date seed exosome freeze-dried powder, hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder, xylitol, lactose, D-mannitol, magnesium stearate, polyvinyl alcohol and maltodextrin into a mixer to mix for 15min to obtain a raw material mixture;
s2, adding 95% ethanol into the raw material mixture, mixing, and then sieving with a 10-mesh sieve to obtain wet particles;
s3, placing the wet particles in a 50 ℃ oven for 3h to obtain dry particles, and then screening the dry particles through a 10-mesh screen to obtain integral particles;
s4, adding magnesium stearate into the whole granules, and totally mixing for 30 min;
s5, tabletting;
s6, dissolving polyvinyl alcohol by using 80% ethanol, and coating tablets;
and S7, bottling the coated tablets.
Comparative example 4
To further illustrate the beneficial effects of the present invention, comparative example 4 is provided, which comparative example 4 differs from example 3 in that: the weight part of the raspberry exosome freeze-dried powder is adjusted to 0.05 part.
Comparative example 5
To further illustrate the beneficial effects of the present invention, comparative example 5 is provided, which comparative example 5 differs from example 3 in that: the raspberry exosome freeze-dried powder is adjusted to be 7 parts by weight.
Comparative example 6
To further illustrate the beneficial effects of the present invention, comparative example 6 is provided, which comparative example 6 differs from example 3 in that: the bitter gourd exosome freeze-dried powder is adjusted to be 1 part by weight.
Comparative example 7
To further illustrate the beneficial effects of the present invention, comparative example 7 is provided, which comparative example 7 differs from example 3 in that: the bitter gourd exosome freeze-dried powder is adjusted to 11 parts by weight.
Comparative example 8
To further illustrate the beneficial effects of the present invention, comparative example 8 is provided, which comparative example 8 differs from example 3 in that: the weight portion of the soybean protein powder is adjusted to 1 portion.
Comparative example 9
To further illustrate the beneficial effects of the present invention, comparative example 9 is provided, which comparative example 9 differs from example 3 in that: the weight portion of the soybean protein powder is adjusted to 12 portions.
Experiment 1: security verification
And (3) testing a sample: examples 1-3 samples prepared
The experimental method comprises the following steps:
10 male and female mice are screened, the male and female mice are raised in cages and weigh 18-22g, the composition for maintaining the ovary, which is prepared by the method of examples 1-3 and contains the raspberry exosome, is ground into powder, the powder is dissolved in purified water and then is taken by the mice, two groups of experiments of a high-dose group and a normal group are set, the observation is carried out for 14 days, relevant data are recorded on 0 day, 7 days and 14 days respectively, and the experimental data are shown in the following table 1 and the following table 2.
TABLE 1 test results of normal acute oral toxicity group
Figure BDA0003083485840000141
TABLE 2 Experimental results for acute oral toxicity high dose group
Figure BDA0003083485840000142
Figure BDA0003083485840000151
From the above results, it can be seen that:
the composition containing the raspberry exosome for maintaining the ovary is provided, and is taken by a mouse at a normal dose or a high dose, the mental state of the mouse is good after the mouse is taken, the behavior of the mouse is normal, the body weight of the mouse is normally increased, no adverse reaction occurs, and the death number of the mouse is 0.
Experiment 2: physical, chemical and microbiological evaluation
And (3) testing a sample: examples 1-3 samples prepared
The experimental method comprises the following steps:
each index was measured according to the standard shown in the column of "control standard" in Table 3.
The experimental data are shown in table 3 below.
TABLE 3 results of physical, chemical and microbiological examinations
Figure BDA0003083485840000152
Figure BDA0003083485840000161
From the above results, it can be seen that:
the results of physical, chemical and microbial evaluation on the raspberry exosome-containing ovarian maintenance composition prepared by the invention show that all physical, chemical and microbial indexes of the raspberry exosome-containing ovarian maintenance composition meet the standard requirements.
Experiment 3: efficacy verification
And (3) testing a sample: samples to be tested prepared in examples 1 to 3 and comparative examples 1 to 9
Subjects and groups: the method comprises the steps of selecting healthy female Kunming mice as study objects, breeding the mice for 8 weeks at a temperature of 22-25 ℃ and a relative humidity of 50-65%, illuminating for 12 hours every day, carrying out adaptive breeding according to normal day and night physiological rules, and freely drinking and eating 12 mice per group. After the adaptive feeding is finished, the experiment dosage is set according to the body weight ratio of the human body to the mouse, the experiment time is set to be 7 days, and the blank control group is filled with distilled water with the same amount as the stomach.
The experimental method comprises the following steps: the levels of serum estradiol (E2) and Follicle Stimulating Hormone (FSH) are measured by adopting an Elisa method, a standard substance and a sample to be measured are added into a transparent enzyme-labeled coating plate which is coated with a mouse estrogen monoclonal antibody in advance, after the incubation is carried out for enough time, the unbound parts are removed by washing, then an enzyme-labeled working solution is added, after the incubation is carried out for enough time, the unbound parts are removed by washing. Adding the substrates A and B in sequence, converting the substrates into blue products under the catalysis of horseradish peroxidase, changing the blue products into yellow under the action of acid, enabling the color depth to be in positive correlation with the concentration of the estrogen of the rat mouse in the sample, measuring the OD value under the wavelength of 450nm, and calculating the E2 value according to the OD values of the sample to be measured and the standard. The calculation of FSH values is similar to E2. The results are shown in Table 4 below.
TABLE 4 mouse serum estradiol (E2) and Follicle Stimulating Hormone (FSH) assays
Figure BDA0003083485840000171
Figure BDA0003083485840000181
From the above results, it can be seen that:
when the raspberry exosome-containing composition for maintaining ovaries is used for feeding mice, the detection value of serum estradiol (E2) of the mice is obviously improved, and the improvement difference is obvious compared with that of a blank control group, so that the raspberry exosome-containing composition for maintaining ovaries has the effect of obviously improving estrogen secretion. The raspberry exosome-containing ovarian maintenance composition has good effects of maintaining ovaries and improving ovarian functions.
When the raspberry exosome-containing ovarian maintenance composition provided by the invention is used for feeding mice, compared with a blank group, the FSH value of all the example groups is obviously reduced, and the FSH value is high, so that endocrine disorders are easily caused, and the raspberry exosome-containing ovarian maintenance composition has the effect of obviously improving the endocrine level, and in the example groups, the effect of improving the endocrine level is the best in example 3. Namely, the raspberry exosome-containing ovarian maintenance composition has a good effect of improving the endocrine level.
After the raspberry exosome-containing composition for maintaining ovaries is not used for feeding mice, the ovaries and the endocrine are not obviously maintained and improved.
It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims (10)

1. The composition containing raspberry exosomes for maintaining ovaries is characterized by comprising the raspberry exosomes, bitter gourd exosomes, spina date seed exosomes, functional raw materials and auxiliary materials.
2. The composition as claimed in claim 1, wherein the functional material is selected from one or more of hawthorn powder, lotus leaf powder, black fungus powder, white gourd seed powder and soybean protein powder.
3. The composition of claim 1, wherein the excipient is selected from one or more of xylitol, lactose, D-mannitol, magnesium stearate, film coating agents, and maltodextrin.
4. An ovarian maintenance product comprising raspberry exosomes, characterized in that it comprises a composition according to any one of claims 1-3.
5. Use of Rubi fructus exosome in ovary care is provided.
6. Use of composition containing Rubi fructus exosome in ovary care is provided.
7. Use of raspberry exosomes in the preparation of ovarian maintenance products.
8. Use of a composition comprising raspberry exosomes in the preparation of an ovarian maintenance product.
9. The composition according to any one of claims 1 to 3 or the use according to any one of claims 5 to 8, wherein the weight fraction of raspberry exosomes is 0.1-5.
10. A method of preparing an ovarian maintenance composition comprising raspberry exosomes, the method comprising the steps of:
s1, adding raspberry exosomes, bitter gourd exosomes, spina date seed exosomes, functional raw materials and auxiliary materials into a mixer, and mixing for 10-20min to obtain a raw material mixture;
s2, adding 85-95% ethanol into the raw material mixture, mixing, and then sieving with a 10-30-mesh sieve to obtain wet particles;
s3, placing the wet granules in a drying oven at 40-80 ℃ for 1-5h to obtain dry granules, and then sieving the dry granules by a sieve of 10-20 meshes to obtain integral granules;
s4, adding magnesium stearate into the whole granules, and totally mixing for 25-35 min.
CN202110573391.6A 2021-05-25 2021-05-25 Composition containing raspberry exosome for maintaining ovary and preparation method thereof Withdrawn CN113288941A (en)

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