CN113279141A - 具有破损皮肤修复功能的纤维敷料及其制备方法 - Google Patents
具有破损皮肤修复功能的纤维敷料及其制备方法 Download PDFInfo
- Publication number
- CN113279141A CN113279141A CN202110614094.1A CN202110614094A CN113279141A CN 113279141 A CN113279141 A CN 113279141A CN 202110614094 A CN202110614094 A CN 202110614094A CN 113279141 A CN113279141 A CN 113279141A
- Authority
- CN
- China
- Prior art keywords
- dressing
- preparation
- damaged skin
- fiber
- fiber dressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000835 fiber Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 claims abstract description 6
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 6
- 229940096998 ursolic acid Drugs 0.000 claims abstract description 6
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001523 electrospinning Methods 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 5
- 238000009987 spinning Methods 0.000 claims abstract description 5
- 229920001661 Chitosan Polymers 0.000 claims abstract description 4
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 230000000694 effects Effects 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 5
- 206010061218 Inflammation Diseases 0.000 abstract description 3
- 230000004071 biological effect Effects 0.000 abstract description 3
- 230000008439 repair process Effects 0.000 abstract description 3
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 241000699670 Mus sp. Species 0.000 description 6
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 230000029663 wound healing Effects 0.000 description 5
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 4
- 230000009102 absorption Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002121 nanofiber Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000586 desensitisation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4282—Addition polymers
- D04H1/4309—Polyvinyl alcohol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/10—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one other macromolecular compound obtained by reactions only involving carbon-to-carbon unsaturated bonds as constituent
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/22—Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
- A61L2300/222—Steroids, e.g. corticosteroids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- Materials Engineering (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Toxicology (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
- Materials For Medical Uses (AREA)
Abstract
本发明属于医用敷料的药物技术领域，具体涉及具有破损皮肤修复功能的纤维敷料及其制备方法。本发明的制备方法包括如下步骤，(1)称取聚乙烯醇溶于30％的乙酸中均匀搅拌为2wt％的溶液，再加入等量的壳聚糖粉末在磁力搅拌器下均匀混合，完成基础的电纺溶液制备；(2)之后加入熊果酸，均匀搅拌；(3)以锡箔纸为基材，进行静电纺丝，纺织完成后的电纺膜静置干燥，制得所述纤维敷料。本发明打破传统外敷材料的理念，将低成本、高治疗效果的中药加入外敷材料中，使其材料具有生物活性从而在外敷过程中发挥抗炎、抗氧化、促修复的作用。
Description
技术领域
本发明属于医用敷料的药物技术领域，具体涉及具有破损皮肤修复功能的纤维敷料及其制备方法。
背景技术
糖尿病患者伤口易感染不易愈合的问题是临床上待解决的棘手问题。目前，治疗糖尿病创面的常规方法是手术清创和包扎创面。传统的纱布或棉垫，在吸收了伤口产生的体液后，会出现纱布干燥的情况。并且，修复过程中，皮肤新生组织会从纱布空隙中生长进去，所以在更换新的纱布时容易造成二次伤害。
至今，随着技术研究的发展，已有许多种类的敷材在市面贩售，例如：凡士林纱布、半渗透膜敷料、透明薄膜式敷料、亲水性凝胶、亲水性敷料、藻酸盐敷料、亲水性纤维敷料、海绵敷料、抗菌敷料、胶原蛋白、接触式敷料(又称作非黏附性敷料)与复合性材料等。传统敷料虽然种类众多，但保湿、抗菌、吸收、促进愈合、生物兼容性、脱敏性仍较差，需要进一步改良。
和传统材料的拉伸、相分离等方法相比，静电纺丝已成为制取纳米纤维最重要、最有效的方法。然而大多数报道的生物材料敷料缺乏生物活性。目前材料中添加促增生的系列生长因子显现出良好的治疗效果，同时生长因子对组织的副作用、递送***的低效性及价格昂贵限制其临床推广。
发明内容
根据现有技术上存在的缺陷，结合目前的研究前沿，本发明提供了具有破损皮肤修复功能的纤维敷料及其制备方法。伤口愈合过程是一个复杂低炎症的慢性疾病过程，本发明加入具有抗菌抗炎的生物活性物质将大大提高材料的性能和促进伤口愈合。
本发明是采用以下的技术方案实现的：
本发明提供了一种具有破损皮肤修复功能的纤维敷料的制备方法，包括如下步骤，
(1)称取聚乙烯醇溶于30％的乙酸中均匀搅拌为2wt％的溶液，再加入等量的壳聚糖粉末在磁力搅拌器下均匀混合，完成基础的电纺溶液制备；
(2)之后加入熊果酸，均匀搅拌；
(3)以锡箔纸为基材，进行静电纺丝，纺织完成后的电纺膜静置干燥，制得所述纤维敷料。
具体的，所述步骤(2)中熊果酸的终浓度为1×10-3～5×10-3g/ml。
其中，所述步骤(3)中静电纺丝的参数设定为电压20KVp,针头与收集器距离为10cm，流速为0.5ml/min，环境温度控制在25-29℃，湿度50-58％R，收集时间为两小时。
本发明还提供一种上述方法制备的具有破损皮肤修复功能的纤维敷料。
与现有技术相比，本发明的有益效果：
本发明打破传统外敷材料的理念，将低成本、高治疗效果的中药加入外敷材料中，使其材料具有生物活性从而在外敷过程中发挥抗炎、抗氧化、促修复的作用，促进伤口加快恢复来缓解病人的痛苦。
附图说明
根据本文给出的发明详述和伴随的附图，将更全面地理解本发明，这些发明详述和附图仅以说明的方式给出，而不限制预期的发明范围。
图1为本发明的操作流程示意图；
图2为实施例1-实施例5中制备的纤维敷料的SEM电镜照片，其中SampleⅠ、Ⅱ、Ⅲ、Ⅳ、Ⅴ分别对应实施例1-实施例5，标尺为1μm；
图3为实施例1-实施例5中制备的纤维敷料的表面张力检测结果图，测试液体为水。
具体实施方式
为了使本发明目的、技术方案更加清楚明白，下面结合附图，对本发明作进一步详细说明(如图1所示)。下述实施例中所述实验方法，如无特殊说明，均为常规方法；实施例中未注明具体技术或条件者，按照本领域内的文献所描述的技术或条件或者按照产品说明书进行；所述试剂和材料，如无特殊说明，均可从商业途径获得。
1、下列实施例采用的材料如下：
生物医药专用静电纺丝机CPS-60K02VIT，购自韩国首尔春巴机电有限公司。
接触角测量仪OCA25，购自北京奥德利诺仪器有限公司。
2、Ⅱ型糖尿病(T2DM)小鼠
4周龄Ⅱ型糖尿病小鼠在12：12小时白昼晚上交替，室温状况下喂养，喂养高糖模型饲料，适应性喂养1周，自由饮食。
一、制备具有破损皮肤修复功能的纤维敷料
实施例1～实施例5
(1)称取0.2g聚乙烯醇溶于10ml 30％的乙酸中均匀搅拌为2wt％的溶液，再加入0.2g的壳聚糖粉末在磁力搅拌器下均匀混合1小时，完成基础的电纺溶液制备；
(2)之后实施例1～实施例5分别加入不同质量的熊果酸(0.01g、0.02g、0.03g、0.04g、0.05g)，均匀搅拌；
(3)以锡箔纸为基材，静电纺丝的参数设定为电压20KVp,针头与收集器距离为10cm，流速为0.5ml/min，环境温度控制在25-29℃，湿度50-58％R，收集时间为两小时，纺织完成后的电纺膜静置干燥。
二、效果检测
1、通过扫描电子显微镜分别对实施例1～5制备的纤维敷料进行表面形貌检测，从图2可以看出，纺织后获得的纳米纤维膜的纤维直径小和纤维间孔径均匀。
2、表面张力检测
利用接触角测量仪，对实施例1～5制备的纤维敷料的表面张力检测，结果如图3所示。可以看出，所有纤维敷料的表面张力随着时间下降，并引起接触角也随着时间发生相应的变化(表面张力降低，接触角减小)。实施例1的纤维敷料的初始接触角角度最大，纤维敷料对水的吸湿性最差；实施例3、4的敷料对水的吸湿性接近，而实施例5的辅料的吸湿性最强。
3、Ⅱ型糖尿病(T2DM)小鼠伤口愈合效果检测
将样本组模型小鼠的伤口处敷贴实施例1～5制备的纤维敷料(每组4只)，对照组小鼠不敷贴任何材料，在伤口愈合前期、中期、后期，分别观察样本组和对照组小鼠，每个样本组小鼠，伤口愈合速度均快于对照组。
当然，以上仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (4)
1.一种具有破损皮肤修复功能的纤维敷料的制备方法，其特征在于，包括如下步骤，
(1)称取聚乙烯醇溶于30％的乙酸中均匀搅拌为2wt％的溶液，再加入等量的壳聚糖粉末在磁力搅拌器下均匀混合，完成基础的电纺溶液制备；
(2)之后加入熊果酸，均匀搅拌；
(3)以锡箔纸为基材，进行静电纺丝，纺织完成后的电纺膜静置干燥，制得所述纤维敷料。
2.根据权利要求1所述的制备方法，其特征在于，所述步骤(2)中熊果酸的终浓度为1×10-3～5×10-3g/ml。
3.根据权利要求2所述的制备方法，其特征在于，所述步骤(3)中静电纺丝的参数设定为电压20KVp,针头与收集器距离为10cm，流速为0.5ml/min，环境温度控制在25-29℃，湿度50-58％R，收集时间为两小时。
4.一种权利要求1-3中任一项所述方法制备的具有破损皮肤修复功能的纤维敷料。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110614094.1A CN113279141A (zh) | 2021-06-02 | 2021-06-02 | 具有破损皮肤修复功能的纤维敷料及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110614094.1A CN113279141A (zh) | 2021-06-02 | 2021-06-02 | 具有破损皮肤修复功能的纤维敷料及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113279141A true CN113279141A (zh) | 2021-08-20 |
Family
ID=77283161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110614094.1A Pending CN113279141A (zh) | 2021-06-02 | 2021-06-02 | 具有破损皮肤修复功能的纤维敷料及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113279141A (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062426A (zh) * | 2006-04-26 | 2007-10-31 | 北京化工大学 | 一种抗菌型共混电纺纳米纤维膜生物敷料及其制备方法 |
| US20110111012A1 (en) * | 2009-11-12 | 2011-05-12 | Hemcon Medical Technologies, Inc. | Nanomaterial wound dressing assembly |
| CN104511045A (zh) * | 2013-09-26 | 2015-04-15 | 五邑大学 | 一种含纳米银的聚乙烯醇/壳聚糖纳米纤维膜敷料及制备 |
| CN107261192A (zh) * | 2017-07-27 | 2017-10-20 | 江南大学 | 一种促进伤口无瘢痕愈合的苦莓苷f1敷料及制备方法 |
| CN108498846A (zh) * | 2018-05-11 | 2018-09-07 | 南通大学 | 一种缓释型壳聚糖抗菌敷料及其制备方法 |
| CN111529748A (zh) * | 2020-04-16 | 2020-08-14 | 东华大学 | 一种医用创面修复用复合敷料及其制备方法 |
-
2021
- 2021-06-02 CN CN202110614094.1A patent/CN113279141A/zh active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062426A (zh) * | 2006-04-26 | 2007-10-31 | 北京化工大学 | 一种抗菌型共混电纺纳米纤维膜生物敷料及其制备方法 |
| US20110111012A1 (en) * | 2009-11-12 | 2011-05-12 | Hemcon Medical Technologies, Inc. | Nanomaterial wound dressing assembly |
| CN104511045A (zh) * | 2013-09-26 | 2015-04-15 | 五邑大学 | 一种含纳米银的聚乙烯醇/壳聚糖纳米纤维膜敷料及制备 |
| CN107261192A (zh) * | 2017-07-27 | 2017-10-20 | 江南大学 | 一种促进伤口无瘢痕愈合的苦莓苷f1敷料及制备方法 |
| CN108498846A (zh) * | 2018-05-11 | 2018-09-07 | 南通大学 | 一种缓释型壳聚糖抗菌敷料及其制备方法 |
| CN111529748A (zh) * | 2020-04-16 | 2020-08-14 | 东华大学 | 一种医用创面修复用复合敷料及其制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Agarwal et al. | Curcumin loaded polycaprolactone-/polyvinyl alcohol-silk fibroin based electrospun nanofibrous mat for rapid healing of diabetic wound: An in-vitro and in-vivo studies | |
| Lee et al. | Core-shell insulin-loaded nanofibrous scaffolds for repairing diabetic wounds | |
| RU2468129C2 (ru) | Биополимерное волокно, состав формовочного раствора для его получения, способ приготовления формовочного раствора, полотно биомедицинского назначения, способ его модификации, биологическая повязка и способ лечения ран | |
| CN104761737B (zh) | 一种静电纺丝法制备胶原蛋白/氧化石墨烯纳米纤维复合膜的方法 | |
| TWI445555B (zh) | 含積雪草活性成分之敷材及其應用 | |
| CN106390177B (zh) | 一种壳聚糖基多层纳米纤维膜敷料及其制备方法和应用 | |
| US5914124A (en) | Alginate fibre, process for the preparation thereof and use | |
| CN110772379B (zh) | 一种负载纳米酶的复合纳米纤维膜的制备方法及其创面敷贴 | |
| Salehi et al. | Kaolin-loaded chitosan/polyvinyl alcohol electrospun scaffold as a wound dressing material: In vitro and in vivo studies | |
| TW200925342A (en) | Method for production of nanofibres | |
| CN107823692B (zh) | 一种创伤敷料复合纳米纤维膜及其制备方法 | |
| CN107130333B (zh) | 一种海藻酸与壳聚糖混编纤维及其制备方法 | |
| CN109381732A (zh) | 负载生长因子小分子抑制剂的静电纺丝敷料、其制备方法及应用 | |
| CN107737364A (zh) | 一种创伤敷料及其制备方法 | |
| PL239827B1 (pl) | Włóknina opatrunkowa i sposób wytwarzania włókniny opatrunkowej | |
| Li et al. | A native sericin wound dressing spun directly from silkworms enhances wound healing | |
| CN104784744A (zh) | 一种人发角蛋白复合伤口敷料的制备方法 | |
| CN111118734B (zh) | 一种聚乙烯醇/羧甲基壳聚糖纳米纤维医用敷料及其制备方法与应用 | |
| Chen et al. | Vapor-phase glutaraldehyde crosslinked waste protein-based nanofiber nonwovens as an environmentally friendly wound dressing | |
| CN113279141A (zh) | 具有破损皮肤修复功能的纤维敷料及其制备方法 | |
| CN110711264B (zh) | 复合材料、医用粘合剂及其制备方法和应用 | |
| TW201323021A (zh) | 生物纖維敷料及其製備方法與用途 | |
| CN109260524B (zh) | 一种组织修复用纳米短纤维材料及其制备方法和应用 | |
| KR20220093701A (ko) | 항염증성을 포함하는 생리활성 기능성을 가지는 콜라겐-히알루론산 복합 천연고분자 기반의 창상피복재용 나노섬유 멤브레인의 제조방법 | |
| Wang et al. | Astragaloside/PVP/PLA nanofiber functional dressing prepared by coaxial electrostatic spinning technology for promoting diabetic wound healing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210820 |
|
| RJ01 | Rejection of invention patent application after publication |