CN113262193B

CN113262193B - Cell signal regulation and development-based uniform skin color brightening essence

Info

Publication number: CN113262193B
Application number: CN202110599117.6A
Authority: CN
Inventors: 朱国君; 罗晓丹; 邢辉
Original assignee: Zhejiang Iseqi Pharmaceutical Technology Co ltd
Current assignee: Zhejiang Iseqi Pharmaceutical Technology Co ltd
Priority date: 2021-05-31
Filing date: 2021-05-31
Publication date: 2022-04-29
Anticipated expiration: 2041-05-31
Also published as: CN113262193A

Abstract

The invention provides a uniform skin color brightening essence developed based on cell signal regulation, which comprises the following components in percentage by mass: 1-12% of water-soluble azelaic acid, 0.1-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 0.5-5% of nano liposome lutein, 1-5% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.01-1% of spirulina maxima extract, 0.1-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water. The brightening essence provided by the invention can inhibit abnormal cell proliferation and development signals activated by inflammatory mediators from the source, prevent melanocytes from transferring excessive and depositing on an epidermal layer to form black spots, improve defense capability by promoting the integrity and compactness of epidermal keratinocyte to recover the normal function of skin cells, remove abnormal denatured protein and prevent protein denaturation.

Description

Cell signal regulation and development-based uniform skin color brightening essence

Technical Field

The invention relates to the technical field of cosmetics, in particular to a uniform skin color brightening essence developed based on cell signal regulation.

Background

Excessive exposure of the skin to various pollutants frequently results in skin sensitization and skin allergic reactions, leading to a significant increase in the production of melanin, which is also an important factor affecting pigmentation. One area where melanin production is beneficial is the absorption of UV from keratinocytes, protecting them. In addition, pigmentation also increases with age. The deposition of excessive skin pigmentation has been one of the researches of worldwide concern, especially asian women mostly look white, pursue a flawless skin color, various whitening products occupy important markets, and more whitening solutions are also needed.

Most of the existing whitening technologies only study a single process of melanin generation, and then perform a point-to-point whitening method, such as inhibiting only the synthesis of tyrosinase. Such technical methods have limited whitening efficacy or cause abnormal pigment loss to cause whitening, and although there are whitening mechanisms for inhibiting melanin transport and stripping melanin, the process of spot formation, especially the process of chemical stripping melanin, cannot be fundamentally explained, and improper use also causes a large amount of inflammatory mediators to be released, and finally the opposite is obtained. In fact, the shade of skin color is not only related to the tyrosinase activity and the melanin synthesis amount. White, fast black, and once deactivated, the skin tone returns to the previous color and is even darker.

Disclosure of Invention

Based on the problems, the brightening essence provided by the invention only plays a role in inhibiting and regulating abnormal melanocytes to restore normal uniform skin color, and focuses on the mechanism of melanin running in the skin and the effect of skin injury repair on uniform skin color.

The technical scheme provided by the invention is as follows:

a uniform skin color brightening essence developed based on cell signal regulation comprises the following components in percentage by mass: 1-12% of water-soluble azelaic acid, 0.1-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 0.5-5% of nano liposome lutein, 1-5% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.01-1% of spirulina maxima extract, 0.1-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

Further, the brightening essence comprises the following components in percentage by mass: 5-12% of water-soluble azelaic acid, 0.5-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 1-5% of nano liposome lutein, 2-5% of diglucosylgallic acid, 0.1-0.2% of VC propylene glycol hyaluronate, 1-3% of water-soluble ellagic acid, 0.01-0.8% of spirulina maxima extract, 0.1-0.8% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

Further, the brightening essence comprises the following components in percentage by mass: 1-10% of water-soluble azelaic acid, 0.1-1.5% of lithospermum stem cells, 0.2-0.4% of lactobacillus ferment cell filtrate, 0.01-0.1% of hydrolyzed conchiolin, 0.5-4% of nano liposome lutein, 1-4% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-2% of water-soluble ellagic acid, 0.2-1% of spirulina maxima extract, 0.2-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

Further, the brightening essence comprises the following components in percentage by mass: 6-8% of water-soluble azelaic acid, 0.8-1.2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 2-3% of nano liposome lutein, 3-4% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.4-0.6% of spirulina maxima extract, 0.4-0.6% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

Further, the humectant comprises glycerin, inositol and isoprene glycol. Wherein the mass ratio of the glycerol to the brightening essence is 3-8%, the mass ratio of the inositol to the brightening essence is 0.5-2.5%, and the mass ratio of the isoprene glycol to the isopentyl glycol is 2-5%.

Further, the stabilizer comprises xanthan gum and sclerotium rolfsii gum. Wherein the xanthan gum accounts for 0.05-0.2% of the brightening essence by mass, and the sclerotium rolfsii gum accounts for 0.1-0.5%.

On the other hand, the invention also provides a preparation method of the brightening essence, which comprises the following steps:

obtaining 1-12 parts of water-soluble azelaic acid, 0.1-2 parts of lithospermum stem cells, 0.2-0.5 part of lactobacillus zymocyte filtrate, 0.01-0.2 part of hydrolyzed conchiolin, 0.5-5 parts of nano liposome lutein, 1-5 parts of diglucosylgallic acid, 0.02-0.2 part of VC propylene glycol hyaluronate, 0.5-3 parts of water-soluble ellagic acid, 0.01-1 part of spirulina maxima extract, 0.1-1 part of tetrahydropyrimidine carboxylic acid, 0.05-0.2 part of sodium phytate, 5.5-15.5 parts of humectant and 0.15-0.7 part of stabilizer; adding water, titrating to 100 parts, and uniformly mixing to obtain the brightening essence.

The brightening essence has the beneficial effects that: can inhibit abnormal cell proliferation and development signals activated by inflammatory mediators from the source, prevent melanocytes from transferring excessive deposition on epidermal layers to form black spots, improve defense capability by promoting the integrity and compactness of epidermal keratinocyte formation cells, recover the normal functions of skin cells, remove abnormal denatured proteins and prevent protein denaturation. The invention clarifies that the spot is a product of inflammation from the perspective of cell biology, and further regulates and lightens melanin from a plurality of inflammation related pathway mechanisms for demonstration, thereby helping the skin to realize brand-new uniform skin color from point to surface and improving the skin transparency.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

Fig. 1 is a comparison graph of the effect of the brightening essence 1 before and after the brightening essence 1 is used in the test 1.

Fig. 2 is a comparison graph of the effect of the brightening essence 2 before and after the test 2.

Fig. 3 is a comparison graph of the effect of the brightening essence 3 before and after the brightening essence 3 is used in the test subject 3.

FIG. 4 is a graph showing the comparison of the results of the experiment for inhibiting melanin production in the fourth example.

FIG. 5 is a statistical chart of the results of the fourth example of the experiment for repairing DNA damage.

FIG. 6 is a graph comparing the results of the fourth example experiment for repairing DNA damage.

FIG. 7 is a graph comparing the results of the fourth example for verification of non-toxicity.

FIG. 8 is a graph showing the statistics of the results of the fourth example experiment for suppressing NF-kB expression.

Fig. 9 is a statistical chart of the results of the experiment affecting eNOS activation of the fourth embodiment.

FIG. 10 is a statistical chart of the results of the fourth embodiment antioxidant performance test.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

From a principle point of view, the synthesis and transport of melanin is a rather complex process. The body produces melanocyte stimulating hormone which acts through melanocyte stimulating hormone MSH receptor on melanocyte membrane to cause mass melanocyte proliferation. The AKT signaling pathway plays an important role in the multi-cellular process, and is also closely related to melanocyte proliferation, cytokines and inflammation associated with melanin deposition. Under the action of exposure group such as ultraviolet rays, melanocytes can over-express receptors on melanin cell membranes, and tyrosinase, protein kinase, melanocortin Prohormone (POMC), etc. trigger the generation or aggregation of melanosome through cell signaling mechanism. Meanwhile, ultraviolet rays can also cause inflammatory reaction of the skin, so that inflammatory factors such as Endothelin (ET), Nitric Oxide (NO) and the like are increased, and melanin synthesis is increased.

More and more researches show that melanocytes and keratinocytes interact in a co-culture process under different conditions, and the metabolism and regulation mechanism of the melanocytes is disclosed. This is a process of interaction, and there are autocrine and paracrine regulatory links between keratinocytes and melanocytes, and cytokines produced between them react with each other, and when different cytokines reach a certain concentration, they act on adjacent cells or act on melanocytes through receptors, for example, basic fibroblast growth factor bFGF, stem cell factor SCF, endothelin ET, leukotriene LT, etc. expressed by keratinocytes can directly act on melanocytes, promote their proliferation and synthesize melanin. The research proves that the two components of the endothelin and the basic fibroblast growth factor can promote the mitosis of human melanocytes, increase the DNA synthesis of the melanocytes, and stimulate the cell proliferation through the divalent calcium ions and inositol phospholipid signal transduction pathways. Two receptor subtypes of endothelin play important roles in the growth and development stages of melanocytes and in the anabolism of melanin. Numerous studies have demonstrated that endothelin can stimulate fibroblast proliferation by endothelin high affinity receptors in fibroblasts, up-regulating bFGF expression.

Melanocytes store synthesized melanin in melanosomes, which are transported by the dendrites of melanocytes to epidermal keratinocytes, giving the skin a color and also causing the skin to be melanized. During melanosis, a melanocyte delivers melanin to approximately 36 keratinocytes, where the dendrites of melanocytes play an important role, transporting melanosomes to the surrounding keratinocytes under the control of tubulin via actin binding. It has been found that in discolored moles, the dendrites of melanocytes are short and the melanin content in keratinocytes is significantly reduced. But after the stimulation of external environmental factors, the growth of the dendrites of the melanocytes is promoted, and the skin color is also deepened. Under the action of environmental factors, type IV collagen, cytokines, hormones and the like can increase the number and the length of dendrites of melanocytes, keratinocytes are an important source of exogenous signals, and secreted endothelin can stimulate the increase and the lengthening of the dendrites of the melanocytes.

In addition, cells are stimulated to activate endothelial nitric oxide synthase, which is primarily responsible for the production of nitric oxide NO, an essential mediator of many cellular functions, and is somewhat destructive. NO, by up-regulating tyrosinase activity and the transcription factor MITF, induces melanosome synthesis, promoting transport of melanosomes to keratinocytes.

The stratum corneum of the skin is also important for optical whitening as the area to which the cosmetic is applied, and proper thickness of the stratum corneum and well-arranged keratinocytes affect the reflection and scattering of light, reducing the amount of light entering the skin and making the skin dull and opaque. The stratum corneum, which is healthy in appearance, acts as a physical barrier, separating the human body from the harmful external environment, and reducing the effects of irritation on melanin is critical.

First, the stratum corneum, the outermost layer of the skin, despite its strong physical barrier function, is constantly disturbed by fluctuating ambient temperature and humidity, ultraviolet rays, microorganisms, daily hygiene, foreign molecules, etc. In order to maintain this vital function of the physical barrier, the epidermis must constantly renew itself, making it the most active part of the human body, with an average daily layer of dead skin cells being shed from the outer surface of the body. And in order to ensure that the skin maintains the barrier function, the epidermis is able to and must function as a "biosensor". The differentiating keratinocytes are able to respond rapidly and compensate for the negative effects of external challenges on the skin barrier function. The immunoreactive capacity of keratinocytes is the second line of defense if the physical barrier function of the skin is considered the first line of defense against the negative effects. Keratinocytes are considered the "prime force" of the epidermis, responsible for daily maintenance tasks. And one epidermal stem cell may be a "mother cell" of 8 to 32 viable keratinocytes. The epidermal stem cells have a long life span and a strong anti-apoptosis mechanism. In this regard, epidermal stem cells behave significantly differently than keratinocytes, which can initiate apoptotic mechanisms when necessary. However, epidermal stem cells are particularly easy to accumulate a large amount of DNA damage, and in young healthy skin, the regeneration process can be smoothly performed, but a natural DNA repair system needs 48 hours to repair 50% of DNA damage, and the regeneration and repair process is slowed down due to external influences such as pressure, sleep deficiency, air-conditioning environment and the like. With age, the rate of renewal is gradually slowed, and the proliferative capacity of epidermal stem cells is diminished and gradually becomes non-functional. The level of cell viability of keratinocytes and epidermal stem cells is closely related to the integrity of DNA in their nuclei. Keratinocytes and epidermal stem cells are exposed to ultraviolet rays from sunlight almost at any time, and thus are particularly vulnerable. For example, when skin is exposed to ultraviolet radiation, DNA in keratinocytes is almost always damaged, and single cells are damaged up to 10 ten thousand times a day. DNA damage caused by sunlight alters the nuclear genomic information of keratinocytes and epidermal stem cells. This alters cellular function and signaling, and results in the release of a number of inflammatory factors, activating the activity of protein kinases, the inflammatory pathway of NF-. kappa.B and MSH.

The intensive research on the complex biological network operation mechanism and the action of a plurality of factors in the complex biological network operation mechanism, especially the control of inflammatory disturbance from the source can realize the efficacy formula of reducing color shading, lightening spots and uniformly brightening skin color to some extent. Therefore, more ideas are provided for solving the problem of lightening the light spots by searching and screening inhibitors of tyrosinase, protein kinase and POMC (polymerase chain reaction) and endothelial nitric oxide synthase antagonist and screening DNA repair enzyme by reducing the stimulation of external environment. The development of effective regulation of melanin bioactivity for stimulated melanocytes is based on the source regulation of protein kinase signaling pathways and melanin regulation mechanisms:

1. inhibiting DNA damage epidermal pigmentation caused by external stimulus including light irradiation. The DNA repair mechanism of skin cells gradually declines with age, and a powerful product must first support keratinocytes and epidermal stem cells, maintain their healthy genome, support their DNA repair mechanism, and provide the additional benefit of increasing skin tolerance to external environmental stimuli.

2. Inhibiting endothelial type carbon monoxide NO synthase, reducing tyrosinase activity and expression of key gene MITF of melanin production, and preventing melanin production process.

3. Reduce the production of inflammatory factors. Skin inflammation is often the primary cause of skin redness, unevenness of the skin, and also produces more additional melanocyte proliferation. Inhibiting NF-kB to reduce inflammation in the color deposition area, blocking proliferation signal of melanocyte, and reducing proliferation of melanocyte. In addition, the active oxygen cluster ROS is inhibited through antioxidation, so that the release of inflammatory mediators is indirectly avoided, and the melanin deposition caused by skin injury is reduced.

4. Accelerate the formation of complete stratum corneum and reduce the external stimulation.

Therefore, based on the above principle, the present invention constructs a formula of the lightening essence: 1-12% of water-soluble azelaic acid, 0.1-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 0.5-5% of nano liposome lutein, 1-5% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.01-1% of spirulina maxima extract, 0.1-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

Wherein, the humectant comprises glycerol, inositol and isoprene glycol, and the mass ratio in the brightening essence is as follows in sequence: 3-8% of glycerol, 0.5-2.5% of inositol and 2-5% of isoprene glycol. The stabilizer comprises xanthan gum and sclerotium rolfsii gum, and the brightening essence sequentially comprises the following components in percentage by mass: 0.05-0.2% of xanthan gum and 0.1-0.5% of sclerotium rolfsii gum.

The functions of the components are as follows:

the water-soluble azelaic acid, the lithospermum stem cells and the lactobacillus zymocyte filtrate hydrolyze conchiolin, can adjust and renew abnormal keratinocytes, simultaneously activate epidermal stem cells, increase cell activity, accelerate epidermal renewal, maintain normal cell cycle, avoid epidermal thinning and prevent abnormal differentiation and proliferation of cells. Abnormal proliferation of cells can lead to the production of large amounts of SCF and bFGF, factors that promote melanocyte proliferation. Supplying keratinocyte, repairing damaged and aged cells, helping the cells resist external environment stimulation, enhancing the natural defense system of skin, improving the tolerance of the skin to the external environment stimulation, and achieving immune homeostasis.

The spirulina maxima extract contains light activated enzyme, can realize rapid repair through light activation-natural light or electronic light, actively cuts Cyclobutane Pyrimidine Dipolymer (CPD) to repair DNA damage caused by radiation, greatly lightens inflammation, stabilizes cells, maintains the stability of epidermal genome, comprehensively and rapidly repairs damage, and restores dynamic balance in tissues.

The hydrolyzed conchiolin can inhibit endothelin secretion, promote involution of involucrin (CE), prevent protein carbonylation and saccharification to brown, and make skin clear and bright.

Lutein, also known as "phytoxanthin", is an oxygen-containing carotenoid, an essential natural antioxidant, and cannot be synthesized by the skin. It can protect the tissue from being damaged by free radicals, absorb harmful rays such as blue light and the like, and reduce the influence of aging; it also stimulates the production of inflammation-related enzymes, such as protein kinases, endothelial nitric oxide synthase, and NF- κ B. Numerous medical studies have shown that: it has excellent anti-inflammatory effect on chronic inflammation-related diseases, and can reduce inflammation activation-related factors from the source, thereby reducing the release of inflammatory factors and further preventing abnormal cell proliferation. Lutein is again a molecule that can combat macular degeneration. According to the technical scheme provided by the invention, the nano-liposome lutein is selected, has an intelligent speckle fading mechanism, only aims at abnormal melanocytes, and particularly plays a role in synthesis of melanosomes, so that the concentration of melanin in the melanosomes can be effectively reduced, and the selectivity of melanin is reduced.

Meanwhile, the biological lutein can be combined with diglucosylgallic acid to strongly inhibit the generation of NF-kB. NF-kB is a strong inflammatory proliferation transduction factor and a growth proliferation signal factor of melanosome cells, which leads to increased melanin synthesis, increased color deposition area and melanin concentration.

The diglucosylgallic acid can combine with the stable VC propylene glycol hyaluronate and the water-soluble ellagic acid to achieve the effect of full-link antioxidation, and simultaneously can resist inflammation, reduce inflammatory disturbance, protect cells, reduce abnormal secretion of melanin, reduce the activity of tyrosinase, reduce and block melanin generation. The water-soluble ellagic acid is a natural polyphenol antioxidant, belongs to a secondary metabolite of a plant, helps to improve the defense capability, has the absorption capability in a 220-nm UV band, and can avoid UV damage from the source.

Fine particles and ultrafine particles, which are air pollution parameters, can cause the expression of Procalcitonin (POMC) in keratinocytes, and POMC expression can also be caused by industrial exhaust gas containing heavy metals and harmful chemicals. Asian human skin is more sensitive to POMC expression. The tetrahydropyrimidic carboxylic acid is very effective in helping Asian human skin to resist pollution, reduces POMC expression and can effectively protect the skin from pigmentation caused by air pollution, such as color spots and excessive pigment deposition.

[ first embodiment ] A method for manufacturing a semiconductor device

The embodiment provides a uniform skin color brightening essence 1 developed based on cell signal regulation, which comprises the following components in percentage by mass: 7% of water-soluble azelaic acid, 1.2% of lithospermum stem cells, 0.4% of lactobacillus zymocyte filtrate, 0.15% of hydrolyzed conchiolin, 0.5% of nano liposome lutein, 4% of diglucosyl gallic acid, 0.15% of VC propylene glycol hyaluronate, 2% of water-soluble ellagic acid, 0.08% of spirulina maxima extract, 1% of tetrahydropyrimidine carboxylic acid, 0.2% of sodium phytate, 8% of glycerol, 1.8% of inositol, 3% of isoprene glycol, 0.1% of xanthan gum, 0.1% of sclerotium rolfsii gum and the balance of water.

Referring to fig. 1, a female of 40 years old is tested 1, chloasma and sunburn are distributed below the eyes and on both sides of the cheeks, and the brightening essence 1 provided by the embodiment is applied to the spot after cleaning the face in the morning and evening, and is obviously improved after 60 days, and only the sunburn which is not completely faded is slightly visible at a position below the cheekbones.

[ second embodiment ]

The embodiment provides a uniform skin color brightening essence 2 developed based on cell signal regulation, which comprises the following components in percentage by mass: 4% of water-soluble azelaic acid, 1.7% of lithospermum stem cells, 0.5% of lactobacillus zymocyte filtrate, 0.18% of hydrolyzed conchiolin, 1% of nano liposome lutein, 2% of diglucosylgallic acid, 0.15% of VC propylene glycol hyaluronate, 3% of water-soluble ellagic acid, 0.05% of spirulina maxima extract, 0.1% of tetrahydropyrimidine carboxylic acid, 0.18% of sodium phytate, 5% of glycerol, 0.7% of inositol, 2% of isoprene glycol, 0.15% of xanthan gum, 0.2% of sclerotium rolfsii gum and the balance of water.

Referring to fig. 2, subject 2 was a 38-year-old female, who was full-face smeared with the brightening essence 2 provided in this example after cleansing in the morning and evening, and after 15 days, the problem of dull skin was improved.

[ third embodiment ]

The embodiment provides a uniform skin color brightening essence 3 developed based on cell signal regulation, which comprises the following components in percentage by mass: 6% of water-soluble azelaic acid, 2% of lithospermum stem cells, 0.5% of lactobacillus zymocyte filtrate, 0.2% of hydrolyzed conchiolin, 2% of nano liposome lutein, 1% of diglucosylgallic acid, 0.2% of VC propylene glycol hyaluronate, 2% of water-soluble ellagic acid, 0.4% of spirulina maxima extract, 0.2% of tetrahydropyrimidine carboxylic acid, 0.16% of sodium phytate, 6% of glycerol, 2.3% of inositol, 3% of isoprene glycol, 0.2% of xanthan gum, 0.3% of sclerotium rolfsii gum and the balance of water.

Referring to fig. 3, in a test 3, the brightening essence 3 provided by the embodiment is applied to the whole face once every day after face cleaning in the morning and evening, the dosage is 0.5ml to 0.8ml each time, and VISIA is used to observe local half-point fading conditions, so that the brightening essence 3 provided by the embodiment has a remarkable effect.

[ fourth example ] A

The embodiment provides a uniform skin color brightening essence 4 developed based on cell signal regulation, which comprises the following components in percentage by mass: 8% of water-soluble azelaic acid, 1.4% of lithospermum stem cells, 0.5% of lactobacillus fermentation cell filtrate, 0.2% of hydrolyzed conchiolin, 3% of nano liposome lutein, 2% of diglucosylgallic acid, 0.2% of VC propylene glycol hyaluronate, 0.5% of water-soluble ellagic acid, 0.5% of spirulina maxima extract, 0.4% of tetrahydropyrimidine carboxylic acid, 0.07% of sodium phytate, 4% of glycerol, 1.2% of inositol, 4% of isoprene glycol, 0.1% of xanthan gum, 0.4% of sclerotium rolfsii gum and the balance of water.

The performance of the brightening essence 4 provided in this example was verified from different angles by experiments.

1. Preventing the generation of melanin.

Experimental group a B16 melanocyte strain, a melanocyte inducer α -MSH and the lightening essence 4 were co-cultured and tested for OD405nm value. The control group was a blank control without the addition of the lightening essence 4. Referring to fig. 4, the left graph is the detection result of the control group, and the right graph is the experimental group, and the comparison shows that the brightening essence 4 can effectively inhibit the generation of melanin.

2. Repair DNA damage.

The degree of DNA damage is determined by comet scanning test (comet assay), and the more small fragments of DNA, the more fragmentation, the longer the tail formed, and the more serious the degree of DNA damage. The experimental group was supplemented with 5% of the brightening essence 4, and the control group was a blank control. Experimental results referring to fig. 5 and 6, it can be seen that the brightening essence 4 provided in this example can effectively repair DNA.

3. And (5) verifying non-toxicity.

As a whitening and lightening product, the cytotoxicity parameter is of great importance, and only compounds with low cytotoxicity are suitable as excellent cosmetics. The lightening essence 4 provided in this example did not affect the survival rate of cells, indicating that it is free from any cytotoxicity. The test method is as follows: the B16 melanocyte strain was co-cultured with various concentrations of lightening essence for 24 hours, and the viability of the cells was tested by MTT method. Referring to fig. 7, the cell survival rate is greater than 80% at different concentrations, which indicates that the cytotoxicity is low.

4. Inhibit NF-kB expression.

Adding alpha-MSH to induce and activate melanin generation state in cultured melanocyte, adding 0.8 μ g/ml of brightening essence 4 in experimental group, and not adding in control group; the amount of NF- κ B expression was then measured by incubating the melanocytes for one hour. Experimental results referring to FIG. 8, the repair serum 4 was effective in inhibiting the expression of NF- κ B.

5. Affecting the activation of eNOS.

The variable 1 is the culture of melanocytes under normal conditions and alpha-MSH induction conditions; variable 2 is using 0.8 μ g/ml of the lightening essence 4 and blank control; in a total of 4 experiments, the activation state of eNOS was determined after one hour incubation. Experimental results referring to fig. 9, the repair essence 4 can affect activation of eNOS.

6. And (5) testing the oxidation resistance.

VC was used as a comparison in this experiment, and the semi-inhibitory concentration (IC50) for scavenging DPPH free radicals was used as an index for evaluation of antioxidant activity. The experimental procedure was as follows: a certain amount of DPPH is weighed and prepared into 0.04mg/mL DPPH solution by using absolute ethyl alcohol. 2mL of 8% brightening essence 4 is added into 2mL of DPPH solution, mixed evenly, placed at room temperature for 30min and centrifuged at 5000r/min for 10 min. The supernatant was collected and absorbance was measured at 517 nm. The control group replaced the brightening essence with VC, and the other conditions were the same. Experimental results referring to fig. 10, the antioxidant capacity of the repair essence 4 provided by the present example is significantly higher than that of VC.

[ fifth embodiment ]

The uniform skin color brightening essence developed based on cell signal regulation and control provided by the invention can further comprise the following components in percentage by mass, and see table 1.

TABLE 1

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A uniform skin color brightening essence developed based on cell signal regulation is characterized by comprising the following components in percentage by mass:

1-12% of water-soluble azelaic acid, 0.1-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 0.5-5% of nano liposome lutein, 1-5% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.01-1% of spirulina maxima extract, 0.1-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

2. The brightening essence of claim 1, comprising the following components in percentage by mass:

5-12% of water-soluble azelaic acid, 0.5-2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 1-5% of nano liposome lutein, 2-5% of diglucosylgallic acid, 0.1-0.2% of VC propylene glycol hyaluronate, 1-3% of water-soluble ellagic acid, 0.01-0.8% of spirulina maxima extract, 0.1-0.8% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

3. The brightening essence of claim 1, comprising the following components in percentage by mass:

1-10% of water-soluble azelaic acid, 0.1-1.5% of lithospermum stem cells, 0.2-0.4% of lactobacillus ferment cell filtrate, 0.01-0.1% of hydrolyzed conchiolin, 0.5-4% of nano liposome lutein, 1-4% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-2% of water-soluble ellagic acid, 0.2-1% of spirulina maxima extract, 0.2-1% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

4. The brightening essence of claim 1, comprising the following components in percentage by mass:

6-8% of water-soluble azelaic acid, 0.8-1.2% of lithospermum stem cells, 0.2-0.5% of lactobacillus ferment cell filtrate, 0.01-0.2% of hydrolyzed conchiolin, 2-3% of nano liposome lutein, 3-4% of diglucosylgallic acid, 0.02-0.2% of VC propylene glycol hyaluronate, 0.5-3% of water-soluble ellagic acid, 0.4-0.6% of spirulina maxima extract, 0.4-0.6% of tetrahydropyrimidine carboxylic acid, 0.05-0.2% of sodium phytate, 5.5-15.5% of humectant and 0.15-0.7% of stabilizer; the balance being water.

5. A lightening essence according to any one of claims 1 to 4, wherein the humectant comprises glycerol, inositol or isoprene glycol.

6. The brightening essence of claim 5, wherein the glycerol is 3-8%, the inositol is 0.5-2.5%, and the isoprene glycol is 2-5% by mass of the brightening essence.

7. A lightening essence according to any one of claims 1 to 4, wherein the stabilizer comprises xanthan gum, sclerotium rolfsii gum.

8. The brightening essence of claim 7, wherein the xanthan gum is 0.05 to 0.2% and the sclerotium rolfsii gum is 0.1 to 0.5% by mass of the brightening essence.

9. A method of preparing a brightening essence according to any one of claims 1 to 8, comprising the steps of:

obtaining 1-12 parts of water-soluble azelaic acid, 0.1-2 parts of lithospermum stem cells, 0.2-0.5 part of lactobacillus zymocyte filtrate, 0.01-0.2 part of hydrolyzed conchiolin, 0.5-5 parts of nano liposome lutein, 1-5 parts of diglucosylgallic acid, 0.02-0.2 part of VC propylene glycol hyaluronate, 0.5-3 parts of water-soluble ellagic acid, 0.01-1 part of spirulina maxima extract, 0.1-1 part of tetrahydropyrimidine carboxylic acid, 0.05-0.2 part of sodium phytate, 5.5-15.5 parts of humectant and 0.15-0.7 part of stabilizer;

adding water, titrating to 100 parts, and uniformly mixing to obtain the brightening essence.