CN113207681B - Breeding method of recessive glandless cotton - Google Patents
Breeding method of recessive glandless cotton Download PDFInfo
- Publication number
- CN113207681B CN113207681B CN202110604007.4A CN202110604007A CN113207681B CN 113207681 B CN113207681 B CN 113207681B CN 202110604007 A CN202110604007 A CN 202110604007A CN 113207681 B CN113207681 B CN 113207681B
- Authority
- CN
- China
- Prior art keywords
- generation
- cotton
- plants
- iii
- genotype
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of agriculture and production thereof, and provides a recessive fertilizerThe gland cotton breeding method comprises the following steps: s1, selecting the genotype as Gl 2 Gl 2 Gl 3 Gl 3 The glandular cotton is female parent and the genotype is gl 2 gl 2 gl 3 gl 3 Taking the recessive glandless cotton as a male parent, and hybridizing to obtain an F1 generation; s2, selfing the F1, and harvesting selfing bolls after maturation to obtain F2; s3, planting F2 generation, and dividing F2 plants into four types, namely I-1 type, I-2 type, II type and III type, in a seedling stage according to the gland density of cotyledon; s4, planting F3 generations in a classified manner, and performing classified identification and screening; s5, repeating the planting method of the S4 in the F4 generation to generate the F5 generation; s6, F5 generation and the planting method of S4 are repeated to generate F6 generation; s7, F6, planting in a classified manner, only retaining the identified III-class single plants, namely gland-free single plants, and performing multi-generation system breeding after single plant selection to obtain new gland-free cotton varieties. By the technical scheme, the problems of low breeding accuracy and low efficiency of the new variety of the glandless cotton in the prior art are solved.
Description
Technical Field
The invention relates to the technical field of agriculture and production thereof, in particular to a breeding method of recessive glandless cotton.
Background
Cotton is an important economic crop, which not only provides fiber raw materials for human beings, but also contains abundant nutrients such as protein, fat, vitamins and the like in the cotton seeds, particularly has quite high content of protein and fat, is an extremely important vegetable oil and an important vegetable protein resource which is only second to soybean and rape in the world, and accounts for 6.9 percent of global protein. The cottonseed protein produced every year can meet the requirement of 5 hundred million people on the protein. However, the glands of the cotton seeds contain gossypol, which easily damages the gastric mucosa of human beings and monogastric animals to cause the disorder of digestive function, thus increasing the difficulty of comprehensively developing and utilizing the cotton seeds and seriously affecting the overall economic benefit of cotton planting. The cultivation of low-phenol cotton variety is an effective technical means for solving the problem of difficult comprehensive utilization of cotton.
The low-phenol cotton varieties cultivated at present are glandless low-phenol cotton varieties, namely glands which are used as gossypol storage places cannot be formed in the cotton, so that the low phenol is realized. Through long-term genetic research, the glandless character of the recessive glandless cotton is mainly controlled by two genes of gl2 and gl3, other genes only play a modifying role, and the cotton is expressed as the glandless cotton only when the two pairs of recessive genes of gl2 and gl3 are homozygously expressed. In the breeding process of the recessive glandless cotton variety, the filial generation can generate 9 plants with different genotypes, and how to accurately identify the genotypes of the different plants is a key for improving the breeding efficiency and accuracy of the glandless cotton and a difficult-to-solve technical problem.
For the existing breeding method, only glandless plants are reserved for breeding, the possibility of genetic variation of cotton is limited, and although invisible glandless cotton can be bred, the quality of the cotton is poor.
Disclosure of Invention
The invention provides a breeding method of a new recessive glandless cotton variety, which solves the problems that in the prior art, the genotype of separated offspring is difficult to distinguish in the breeding process of the recessive glandless cotton variety, and the breeding accuracy and efficiency of the new glandless cotton variety are low.
The technical scheme of the invention is as follows:
a method for breeding recessive glandless cotton comprises the following steps:
s1, selecting the genotype as Gl 2 Gl 2 Gl 3 Gl 3 The glandular cotton is female parent and the genotype is gl 2 gl 2 gl 3 gl 3 Taking the recessive glandless cotton as a male parent, and hybridizing to obtain an F1 generation;
s2, selfing the F1 generation, and harvesting selfing bolls after maturation to generate an F2 generation;
s3, planting F2 generation, and classifying F2 plants into I, II and III according to a classification standard I in the seedling stage, wherein the classification standard I is as follows:
class I: the cotyledon glands are more and evenly distributed, and the gland density>90Per cm 2 ；
And II: the cotyledon glands are few and only distributed at the edge of cotyledon, 0 cotyledon/cm 2 <Gland density<50 pieces/cm 2 ；
Class III: the cotyledon has no gland, and the gland density is 0/cm 2 ；
Classifying class I into two classes I-1 and I-2 according to classification standard II according to gland density of mature true leaf,
class I-1: the number of true-leaf glands is large and the glands are distributed more uniformly, and the gland density is more than 90/cm 2 ；
Class I-2: the true leaf glands are sparse and unevenly distributed, and the gland density is less than 80 per cm 2 ；
Screening criteria one: eliminating the I-1 plants, reserving the I-2, II and III plants for selfing, harvesting the I-2 plants after maturation by single plant division, and harvesting the II and III plants in a mixed manner to generate an F3 generation;
s4, planting F3 generations, screening according to classification standards 1 and 2, reserving I-2, II and III, obtaining respective F4 generations for each category according to a screening standard II, wherein the screening standard II is as follows:
class I-2: in the seedling stage eliminated line, cotyledons of each single plant are all glandular lines, the line produced by the F2 single plant containing the glandless single plant is subjected to single plant selection according to the first classification standard and the second classification standard of the F2 and the first screening standard, and selected single plants are selfed to produce F4 generations;
and II: selecting single plants according to the first classification standard, the second classification standard and the first screening standard of the F2 at the seedling stage, and selfing selected single plants to generate F4 generations;
selfing all III to generate F4 generation;
s5, repeating the planting method of the S4 in the F4 generation to generate the F5 generation;
s6, F5 generation and the planting method of S4 are repeated to generate F6 generation;
and S7, planting F6 generation by classification, performing single plant selection by only reserving class III single plants in the seedling stage according to the classification standard, performing multi-generation systematic breeding until the characters meet the variety requirements, and breeding the non-glandular cotton.
As a further technical scheme, the genotype of the I-type plant is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 、Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 、Gl 2 gl 2 gl 3 gl 3 To (3) is provided.
As a further technical scheme, the genotype of the II-type plant is Gl 2 gl 2 gl 3 gl 3 、gl 2 gl 2 Gl 3 gl 3 One kind of (1).
As a further technical scheme, the type III genotype is gl 2 gl 2 gl 3 gl 3 。
As a further technical scheme, the genotype of the I-1 plant is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 One kind of (1).
As a further technical scheme, the genotype of the I-2 plant is Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 One kind of (1).
As a further technical solution, in the step S7, the criteria for individual plant selection are: the average length of the upper half part of the fiber is more than 27mm, the breaking specific strength is more than 28cN/tex, the micronaire value is 3.5-5.5, the single bell weight is more than 5.5g, the clothes mark is more than 38.0 percent, and the disease grade of the blight is below grade 2.
As a further technical solution, in the step S7, the variety requirement specifically is: the wilt disease is less than 5, the verticillium wilt disease is less than 20, the average length of the upper half is more than or equal to 27mm, the fracture ratio strength is more than or equal to 28cN/tex, and the micronaire value is 3.5-5.5.
The invention has the beneficial effects that:
1. the invention provides a method for breeding a new variety of glandless cotton, aiming at the problem that the genotype of separated offspring is difficult to distinguish in the breeding process of a recessive glandless cotton variety, and the separated offspring is accurately identified and classified based on the time-space distribution of gland density, and is classified, so that more genetic variation information can be stored, and the breeding accuracy and efficiency of the new variety of glandless cotton can be improved. The gland density can well represent the genotype of a plant, and is more visual and more convenient for field operation compared with the genotype, so that the invention provides a method for breeding a new variety of non-gland cotton based on gland density spatial-temporal distribution, which is feasible and effective.
2. When F2 generation separation single plants are screened and identified, the invention divides the single plants into four types of I-1, I-2, II, III and the like based on the gland density of cotyledon and true leaf, only eliminates the type I-1, and retains the type I-2, II and III, compared with the traditional method of retaining the type III of target character, the invention can retain more genetic variation, and effectively improves the success rate of selection and breeding of non-gland varieties. Different generations sort and screen the classification and plant, compare and mix the mixed species of choosing, can reduce the genotype of the high generation colony and appraise the difficulty screened, effectively promote the selection accuracy and efficiency of the single plant of segregation progeny. On the other hand, the invention limits the breeding standards of four types of I-1, I-2, II and III, and improves the success rate of breeding.
Drawings
The invention is described in further detail below with reference to the drawings and the detailed description.
FIG. 1 shows the distribution of cotyledon glands of types I, II and III in example 1;
FIG. 2 shows the glandular distribution of true leaves of types I-1 and I-2 in example 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any inventive step, are intended to be within the scope of the present invention.
Example 1
(1) In the Hainan area in 2010, selecting high-yield high-quality gland-containing conventional cotton Ji cotton 616 with good disease resistance as a female parent and a recessive gland-free cotton resource far 582 with excellent comprehensive performance as a male parent, preparing a hybrid combination, and harvesting a hybrid boll after boll opening to obtain seeds of F1; wherein the cotton wing cotton 616 has been disclosed in "high-yield disease-resistant cotton new product wing cotton" (Cui Shufang, li Junlan, jin Weiping, etc. high-yield disease-resistant cotton new product wing cotton 616[ J ]. Chinese cotton, 2008,035 (009): 23.), wherein the recessive glandless cotton flower resource far 582 line is selected from distant hybridization offspring, the content of free gossypol in the cotton seeds is 0.012%, the whole growth period is 119d, the plant height is about 96.1cm, the number of fruit branches is 12.3, the number of single plant bolls is 23.9, the boll weight is 6.0g, the clothing branch is 43.6%, the wilt disease finger is 6.8, and the verticillium wilt disease finger is 32.1.
(2) Planting F1 in Shijiazhuang in 2011, selfing, and harvesting selfed bolls after maturation to obtain F2;
(3) F2 generations are planted in south of Hainan in winter in 2011, F2 plants are divided into three types (I type, II type and III type) in the seedling stage according to the distribution and density of cotyledon glands, and different types of plants are marked by hair lines with different colors. Based on the distribution and density of glands of true leaves, the class I is subdivided into two classes I-1 and I-2, and the class I-1 plants are eliminated. Selfing reserved I-2, II and III plants, collecting I-2 plants in a single plant after boll opening, and collecting II plants and III plants in a mixed manner to generate an F3 generation;
class I: the cotyledon glands are more and evenly distributed, and the gland density>90 pieces/cm 2 The genotype of the plant is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 、Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 、Gl 2 gl 2 gl 3 gl 3 ；
And II: the number of cotyledon glands is small and only distributed at the edge of cotyledon, 0 cotyledon/cm 2 <Gland density<50 pieces/cm 2 The plant gene of the classType is Gl 2 gl 2 gl 3 gl 3 、gl 2 gl 2 Gl 3 gl 3 ；
Class III: the cotyledon has no gland, and the gland density is 0/cm 2 Genotype is gl 2 gl 2 gl 3 gl 3 The plant of (a); after classification, utilizing the knitting wool with different colors to mark plants of different categories;
class I-1: more true-leaf glands and more uniform distribution, gland density>90 pieces/cm 2 (ii) a Genotype is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 ；
Class I-2: the true leaf glands are sparse and unevenly distributed, and the gland density<80 pieces/cm 2 Genotype is Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 。
(4) F3 generations, I-2 types and plants are planted in the Shijiazhuang in a classified manner in 2012, plants are planted into plant lines in a single plant mode, identification and screening are carried out in a seedling stage according to the density of cotyledon glands, plant lines with glands in each single plant cotyledon in the plant lines are eliminated, plant lines containing gland-free single plants (plant lines generated by Gl2Gl2Gl3Gl3 genotype F2 single plants) are selected in a single plant mode according to the identification and screening method of F2, and the selected single plants are selfed to generate F4 generations; II, identifying and screening according to the density of the cotyledon glands in the seedling stage, eliminating the single plants with the density of I-2 glands, keeping the single plants with the densities of II-type and III-type glands, and selecting the single plants for selfing to generate F4 generations; class iii selfing produces F4.
(5) F4 was planted in the south hainan classification in winter 2012, and F4 repeated the screening and identification method of F3, resulting in F5.
(6) F5 was planted in the Shijiazhuang classification in 2013, and the screening and identification method of F3 was repeated to yield F6.
(7) F6 is planted in Hainan in winter in 2013 in a classified mode, only the identified III-type single plants, namely glandless single plants, are reserved in the seedling stage according to the density of cotyledon glands, the single plants are further screened by a conventional method according to the principle that the average length of the upper half part of fibers is greater than 27mm, the breaking ratio strength is greater than 28cN/tex, the micronaire value is 3.5-5.5, the single boll weight is greater than 5.5g, the clothes weight is greater than 38.0%, and the disease level of the withered yellow disease is less than 2, and the recessive glandless cotton flower Ji cotton 9218 is bred in 2017. Ji cotton 9218 has completed the low gossypol cotton regional test in Hebei province, reaches the approval requirement, has applied for approval in 2021. The specific field test results are as follows: the average result of the two-year area test in 2019-2020 is that the free gossypol content in the cottonseed is 0.010085%, the clothes content is 40.4%, and the weight of a single bell is 5.6g. The average yield per mu of the ginned cotton before frost is 97.55kg and 90.52kg respectively, and the ginned cotton yield is increased by 6.55 percent and 6.48 percent respectively compared with a control (Handan without 198). The average length of the upper half is 30.7mm, the breaking strength is 30.2cN/tex, and the micronaire value is 4.95. The identification of the plant protection research institute of agriculture and forestry academy of sciences of Hebei province in 2019 shows that the wilt disease index is 0.20, and the relative verticillium wilt disease index is 11.28, and the wilt disease index belongs to the type of wilt disease resistance and verticillium wilt resistance. Identified by plant protection research institute of agriculture and forestry academy of sciences of Hebei province in 2020, the fusarium wilt index is 6.09, and the verticillium wilt relative disease index is 15.39, and the anti-fusarium wilt and anti-verticillium wilt disease type belongs to. In the 2020 production test, the yield per mu of the unginned cotton, the ginned cotton and the ginned cotton before frost is 230.38kg, 94.18kg and 87.35kg respectively, and the yield is increased by 5.71%, 8.73% and 10.01% respectively compared with that of the control Handan.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. A breeding method of recessive glandless cotton is characterized by comprising the following steps:
s1, selecting the genotype as Gl 2 Gl 2 Gl 3 Gl 3 The glandular cotton is female parent and the genotype is gl 2 gl 2 gl 3 gl 3 Taking the recessive glandless cotton as a male parent, and hybridizing to obtain an F1 generation;
s2, selfing the F1 generation, and harvesting selfing bells in a mixed mode after maturation to generate an F2 generation;
s3, planting F2 generation, and classifying F2 plants into I, II and III according to a classification standard I in the seedling stage, wherein the classification standard I is as follows:
class I: the cotyledon glands are more and evenly distributed, and the gland density>90 pieces/cm 2 ；
And II: the cotyledon glands are few and only distributed at the edge of cotyledon, 0 cotyledon/cm 2 <Gland density<50 pieces/cm 2 ；
Class III: the cotyledon has no gland, and the gland density is 0/cm 2 ；
Classifying class I into two classes I-1 and I-2 according to classification standard II according to gland density of mature true leaf,
class I-1: the number of true-leaf glands is large and the glands are distributed more uniformly, and the gland density is more than 90/cm 2 ；
Class I-2: the true leaf glands are sparse and unevenly distributed, and the gland density is less than 80 per cm 2 ；
Screening criteria one: eliminating the I-1 plants, reserving the I-2, II and III plants for selfing, harvesting the I-2 plants after maturation by single plant division, and harvesting the II and III plants in a mixed manner to generate an F3 generation;
s4, planting F3 generations, screening according to classification standards 1 and 2, reserving I-2, II and III, obtaining respective F4 generations for each category according to a screening standard II, wherein the screening standard II is as follows:
class I-2: in the seedling stage eliminated line, cotyledons of each single plant are all glandular lines, the line produced by the F2 single plant containing the glandless single plant is subjected to single plant selection according to the first classification standard and the second classification standard of the F2 and the first screening standard, and selected single plants are selfed to produce F4 generations;
and II: selecting single plants according to the first classification standard and the second classification standard of the F2 and the first screening standard in the seedling stage, and performing selfing on the selected single plants to generate F4 generations;
selfing all III to generate F4 generation;
s5, repeating the planting method of the S4 in the F4 generation to generate an F5 generation;
s6, F5 generation and the planting method of S4 are repeated to generate F6 generation;
s7, planting F6 generation by classification, only keeping III type single plants for single plant selection in the seedling stage according to classification standards, breeding by a multi-generation system until the characters meet the variety requirements, and breeding into gland-free cotton;
the genotype of the I-type plant is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 、Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 、Gl 2 gl 2 gl 3 gl 3 One of (a) and (b);
the genotype of the II type plant is Gl 2 gl 2 gl 3 gl 3 、gl 2 gl 2 Gl 3 gl 3 One of (1);
the type III genotype is gl 2 gl 2 gl 3 gl 3；
The genotype of the I-1 plant is Gl 2 Gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 Gl 3 gl 3 、Gl 2 gl 2 Gl 3 Gl 3 One of (1);
the genotype of the I-2 plant is Gl 2 gl 2 Gl 3 gl 3 、gl 2 gl 2 Gl 3 Gl 3 、Gl 2 Gl 2 gl 3 gl 3 One kind of (1).
2. The method for breeding the recessive glandless cotton according to claim 1, wherein in the step S7, the criteria for selecting the single plant are as follows: the average length of the upper half part of the fiber is more than 27mm, the breaking specific strength is more than 28cN/tex, the micronaire value is 3.5-5.5, the single bell weight is more than 5.5g, the clothes mark is more than 38.0 percent, and the disease grade of the blight is below grade 2.
3. The method for breeding the recessive glandless cotton according to claim 1, wherein in the step S7, the variety requirements are as follows: the wilt disease is less than 5, the verticillium wilt disease is less than 20, the average length of the upper half is more than or equal to 27mm, the fracture ratio strength is more than or equal to 28cN/tex, and the micronaire value is 3.5-5.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110604007.4A CN113207681B (en) | 2021-05-31 | 2021-05-31 | Breeding method of recessive glandless cotton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110604007.4A CN113207681B (en) | 2021-05-31 | 2021-05-31 | Breeding method of recessive glandless cotton |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113207681A CN113207681A (en) | 2021-08-06 |
| CN113207681B true CN113207681B (en) | 2022-10-28 |
Family
ID=77081849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110604007.4A Active CN113207681B (en) | 2021-05-31 | 2021-05-31 | Breeding method of recessive glandless cotton |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113207681B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115039689A (en) * | 2022-05-09 | 2022-09-13 | 河北省农林科学院旱作农业研究所 | Method for breeding and propagating cotton hybrid by using marker character |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264401C (en) * | 2003-11-10 | 2006-07-19 | 中国农业科学院棉花研究所 | Seed production method of cotton sterile line |
| US8614375B2 (en) * | 2009-12-04 | 2013-12-24 | Phytogen Seed Company | Cotton variety |
| CN102499060A (en) * | 2011-11-01 | 2012-06-20 | 江苏省农业科学院 | Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method |
| CN102613067A (en) * | 2012-02-29 | 2012-08-01 | 河南省农业科学院 | Cotton hybrid breeding method utilizing double labeling method |
| CN102640591A (en) * | 2012-02-29 | 2012-08-22 | 河南省农业科学院 | Seedling identification method of purity of palmatum leaf pigment gland double labeled hybrid cotton seeds |
-
2021
- 2021-05-31 CN CN202110604007.4A patent/CN113207681B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113207681A (en) | 2021-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109463273B (en) | Efficient breeding method for super-early-maturing disease-resistant cotton | |
| CN109997682B (en) | Breeding method for hybrid polymerized cotton with high-quality characters | |
| CN112167054B (en) | Breeding method of new transgenic high-quality upland cotton variety | |
| CN106665332A (en) | Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material | |
| CN113207681B (en) | Breeding method of recessive glandless cotton | |
| CN110169355B (en) | Cultivation method of summer direct seeding cotton variety | |
| Muminov et al. | Analysis of yield and fiber quality traits in intraspecific and interspecific hybrids of cotton | |
| CN111328701A (en) | Cultivation method of high-quality rice restorer line | |
| Fu et al. | Assessment of the genetic diversity and population structure of lotus cultivars grown in China by amplified fragment length polymorphism | |
| CN108782225B (en) | Breeding method of high-yield early-maturing high-quality multi-resistance cotton | |
| Muminov et al. | Cluster analysis of valuable economic traits in amphidiploid cotton hybrid plants. | |
| CN114946643B (en) | Short-season cotton continuous spring and summer carousel polymerization breeding method | |
| CN109601370B (en) | Cascade selection method suitable for breeding new tobacco strain | |
| CN104770290A (en) | Multi-parent seed reserving and selective breeding method of Taishanshuhong peaches | |
| CN110679472B (en) | Method for breeding new corn variety by using X group and Huanglan group heterosis mode | |
| CN110679476B (en) | Innovative breeding method of Huangrui group germplasm | |
| CN112673954B (en) | High-yield breeding method of sesame with limited growth habit | |
| CN101642022A (en) | Method for culturing cotton resisting micro-bundle disease | |
| CN114831021A (en) | Ornamental rape variety cultivation method suitable for miniature bonsai | |
| Musango et al. | Phenotypic characterization of common bean (Phaseolus vulgaris L.) accessions conserved at the genetic resources and biotechnology institute | |
| CN108124758B (en) | Method for purifying and rejuvenating local variety of coix lacryma-jobi and breeding new variety | |
| CN110122136A (en) | A kind of method and its application of each colour system tea tree breed mixed planting | |
| CN111149687A (en) | Method for cultivating cotton variety suitable for thin, dense-tolerant and rot-resistant bolls in thin and dense alternating environment | |
| CN105010131A (en) | Operation method for salvia splendens EMS(Ethyl methane sulfonate) mutation breeding | |
| CN110447530B (en) | Breeding method of light green leaf three-line sterile line, indica-japonica intermediate restorer line and indica-japonica subspecies hybrid rice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |