CN113081890A

CN113081890A - Essence with anti-sugar and anti-oxidation functions and preparation method thereof

Info

Publication number: CN113081890A
Application number: CN202110456744.4A
Authority: CN
Inventors: 杨钰澍; 贾洪雷
Original assignee: Shanghai Fulai Biological High Tech Co ltd
Current assignee: Shanghai Fulai Biological High Tech Co ltd
Priority date: 2021-04-27
Filing date: 2021-04-27
Publication date: 2021-07-09

Abstract

The invention discloses an essence with anti-sugar and anti-oxidation functions and a preparation method thereof, wherein the effective components of the essence comprise an antioxidant and an anti-saccharification agent, the antioxidant is a mixture of carnosine, inositol, yeast lysate extract, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol, the anti-saccharification agent is decarboxylated carnosine HCl, and other functional active components are selected and compounded by adopting various functional components, and the essence can inhibit the release of inflammatory factors, promote the expression of related genes of barrier repair, repair skin barriers, delay aging, repair injury and activate the vitality of fibroblasts by eliminating skin oxygen free radicals, inhibiting glycosylation and treating glycosylated protein for oxidation and saccharification. According to the invention, the anti-saccharification component, the antioxidant component and the other active components are reasonably compounded, so that the activity of each component is improved, and the essence has good anti-sugar and antioxidant effects.

Description

Essence with anti-sugar and anti-oxidation functions and preparation method thereof

Technical Field

The invention belongs to the technical field of daily cosmetics, and particularly relates to essence with sugar-resisting and oxidation-resisting functions and a preparation method thereof.

Background

Glycation is a physiological phenomenon, which refers to the addition of a reducing amino group at the end of a protein in vivo and an excess of an aldehyde group in a reducing sugar molecule to form reversible Schiff bases (Schiff bases), and then the rearrangement with glucosamine (Amadori) to form an early glycosylation product. The early glycosylation products are subjected to a series of chemical reactions such as dehydration condensation, rearrangement, oxidation, crosslinking and the like to finally generate advanced glycosylation end products (AGEs). With aging, metabolism of the skin becomes slow, which causes accumulation of Advanced Glycation Endproducts (AGEs) in the skin, leading to an increase in the skin renewal cycle, a decrease in the metabolic function of skin regeneration, and accelerated aging of the skin. The oxidation is mainly aging caused by skin influenced by environmental factors, mainly takes the influence of ultraviolet radiation as a main factor, and with the development of science and technology, modern people live in a digital environment at any moment, so that the skin is exposed to mobile phones, televisions, computers, various illuminating lamps and the like and is continuously radiated by blue light. When the skin is exposed to long-wave Ultraviolet (UVA), medium-wave Ultraviolet (UVB) and blue light for a long time, the skin generates Reactive Oxygen Species (ROS), which causes a series of oxidative damages, resulting in mitochondrial damage and apoptosis, DNA strand breakage in cell nucleus, stimulation of cell secretion of a large amount of inflammatory factors to induce inflammatory reaction, collagen synthesis reduction, skin barrier destruction, etc., and accelerates skin aging. In addition, after a large amount of free radicals generated by oxidation are accumulated in vivo, the free radicals also participate in the saccharification reaction, early glycosylation products can be changed back to sugar and protein through a reversible reaction, but after the early glycosylation products encounter the free radicals, the formation of AGEs is accelerated under the action of the free radicals.

In order to solve the problems, various anti-glycation and anti-oxidation oral and external products are successively developed in the prior art, and Chinese patent application 201910281558.4 discloses a skin care product with five-dimensional anti-wrinkle and anti-aging effects, wherein the skin care product comprises nicotinamide, membrane-coated yeast fermentation product filtrate, centella asiatica extract, beta-glucan, pomegranate fruit extract, rice extract, ceramide 3, palmitoyl tripeptide-5, acetyl hexapeptide-8, dimethyl silanol hyaluronate and other components, so that the adhesion degree of epidermis and dermis layers can be increased, the synthesis of collagen is promoted, nerves are relieved, expression lines are reduced, silicon is supplemented, the elasticity of skin is improved, glycation is prevented, and the skin is improved in five dimensions to achieve the effects of resisting wrinkle and aging. However, the formulation has no antioxidant effect, and due to the inseparable relationship between saccharification and oxidation, the product has difficulty in achieving optimal anti-saccharification and antioxidant requirements. Chinese patent publication CN109125205A, utilizes antioxidant vitamin C ethyl ether, fullerene derivatives to eliminate Reactive Oxygen Species (ROS) and free radicals to achieve anti-aging effect; however, it is difficult to achieve anti-glycation and fails to meet the requirement of anti-aging in all aspects. Although various active ingredients are usually combined in various skin care products to achieve better anti-sugar or anti-oxidation effects, the requirements of anti-saccharification and anti-oxidation cannot be met simultaneously, and the traditional Chinese medicine has no synergistic effect among the active ingredients and cannot achieve the expected optimal anti-aging effect.

Therefore, how to provide a multi-component synergistic product with anti-sugar and anti-oxidation functions is a problem to be solved by those skilled in the art.

Disclosure of Invention

In view of the above, the invention provides the formula of the essence with the functions of resisting sugar and resisting oxidation and the preparation method thereof, and the activities of the anti-saccharification component and the anti-oxidation component are reasonably compounded to improve the activities of the anti-saccharification component and the anti-oxidation component, so that the essence has good effects of resisting sugar and resisting oxidation simultaneously, and has the best anti-aging effect.

In order to achieve the purpose, the invention adopts the following technical scheme:

the essence with the functions of resisting sugar and oxidation comprises the following components in parts by weight:

40-70 parts of deionized water, 0.1-3 parts of emulsifier, 5-20 parts of humectant, 0.3-5 parts of antioxidant, 0.6-5 parts of anti-saccharification agent, 0.1-1 part of soothing agent, 0.1-1 part of preservative, 0.1-1 part of thickener and 2-10 parts of oily component.

Preferably, the essence with the functions of resisting sugar and oxidation comprises the following components in parts by weight:

60 parts of deionized water, 1.7 parts of emulsifier, 15 parts of humectant, 2.9 parts of antioxidant, 3.8 parts of anti-saccharification agent, 0.7 part of soothing agent, 0.2 part of preservative, 0.7 part of thickening agent and 6 parts of oily component.

Preferably, the emulsifier is a mixture of cetearyl olive oleate and sorbitan olive oleate.

The effect of the above preferred scheme is: cetearyl olive oleate is used as emulsifier, has dispersing effect, and can be used for two components which are not dissolved in water and oil to make one component uniformly dispersed in the other component to form emulsion or suspension, and has high safety for cosmetic; sorbitan olivate is also a safe and good emulsifier; the combination of multiple emulsifiers ensures good compatibility of the components.

Preferably, the humectant is a mixture of butylene glycol, glycerin polymethacrylate, glyceryl polyether-26, isoprene glycol, trehalose, sodium hyaluronate, hydrolyzed hyaluronic acid, and arginine.

The beneficial effects of the preferred technical scheme are as follows: butanediol, glycerin, sodium hyaluronate, hydrolyzed hyaluronic acid and arginine are all commonly used good moisturizers; the glycerin polymethacrylate is a liquid with particularly high moisture-retaining components, is a water-soluble transparent gel, and can be used as a moisture-retaining matrix to increase skin feel and lubricity and improve moisture retention; the glyceryl polyether-26 is obtained by polymerizing glycerin and ethylene oxide, and has certain dispersion promoting effect as humectant of cosmetic, which is beneficial for uniformity of finished product; the isoprene glycol has the functions of moistening the skin and moisturizing, also has certain relieving and antibacterial properties, and reduces the addition of preservatives; trehalose has strong moisturizing effect and physiological effects of sun protection, ultraviolet protection and the like, and can not only moisturize but also assist in resisting ultraviolet oxidation.

Preferably, the antioxidant is a mixture of carnosine, inositol, yeast lysate extract, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol.

The beneficial effects of the preferred technical scheme are as follows: carnosine has antioxidant property, is a natural antioxidant, is safe and has no side effect; inositol is widely present in various natural animal, plant and microorganism tissues, is a growth factor of animals, plants and microorganisms, has very good moisturizing and conditioning functions on skin due to the same action of biotin and vitamin B, namely has good relieving action while moisturizing and has excellent antioxidant effect; the extract of the yeast lysate contains a plurality of nutrient substances, and amino acids, nucleosides, nucleotides, peptides, active enzymes, amino acid precursors, sterols, trace elements, vitamin B families and the like with high proportion in the lysate are basically active substances which can be directly utilized, so that basic nutrition is fully provided for skin cells, melanin generation can be inhibited, the respiration effect of the cells is enhanced, the oxygen consumption efficiency of the cells is improved, the formation of active oxygen free radicals is reduced, and a plurality of accessory enzyme components can reduce the damage of free radical oxidation to the skin and have excellent antioxidation; the Haematococcus pluvialis extract mainly contains astaxanthin, which is a chain-breaking antioxidant. The antioxidant has strong antioxidant capacity, can eliminate nitrogen dioxide, sulfide, disulfide and the like, can reduce lipid peroxidation, and effectively inhibit lipid peroxidation caused by free radicals; the auxiliary antioxidant function of oryzanol and tocopherol is combined, so that the component achieves the optimal antioxidant effect.

Preferably, the anti-glycation agent is decarboxypeptide HCl.

The beneficial effects of the preferred technical scheme are as follows: the decarboxylated carnosine has the effects of capturing hydroxyl free radicals, simple substance oxygen and hydrogen peroxide free radicals, can inhibit lipid oxidation caused by nonmetallic ions, can stably protect cell membranes, resists oxidation, and inhibits glycosylation of structural proteins through two different target points to protect collagen and elastin.

Preferably, the soothing agent is a mixture of panthenol, agaricus bisporus extract and allantoin.

The beneficial effects of the preferred technical scheme are as follows: panthenol can be converted into pantothenic acid (VI 3s) in skin, is equivalent to pantothenic acid, and has moisturizing, antiinflammatory, and cell division stimulating effects; the agaricus bisporus extract contains extremely many amino acids, nucleotides and vitamins, can moisten and care skin for comprehensively conditioning skin, and also has good antioxidant, sedative, anti-inflammatory and bacteriostatic effects; allantoin is an amphoteric compound, can combine with multiple substances to form double salt, and has light-shielding, antibacterial, antiseptic, analgesic, and antioxidant effects.

Preferably, the preservative is a mixture of 1, 2-hexanediol, p-hydroxyacetophenone and dipotassium glycyrrhizinate.

The beneficial effects of the preferred technical scheme are as follows: the 1, 2-hexanediol has antibacterial activity, is not influenced by hydrolysis, temperature and pH value, replaces the traditional preservative, has skin moistening and moisturizing effects, and is mild and non-irritant; the p-hydroxyacetophenone is a high-temperature auxiliary activity stabilizer, has stable component properties, improves the component stability and has no side effect on the skin; the dipotassium glycyrrhizinate has good antibacterial effect.

Preferably, the thickener is a mixture of carbomer and xanthan gum.

Preferably, the oily component is a mixture of isononyl isononanoate, squalane and palm kernel oil.

The beneficial effects of the preferred technical scheme are as follows: the isononyl isononanoate has a unique multi-methyl branched chain structure, has good intermiscibility to oily components, can solve the problem of low-temperature precipitation of the oily components, is a good emulsifier, a good stabilizer and a good coupling agent, and has good dispersion effect on various raw materials; squalane is animal oil, has good affinity to skin, does not cause allergy and irritation, and can accelerate other active ingredients in the formula to permeate into skin; the skin care cream has low polarity and moderate spreadability, can inhibit the growth of mould, strengthen and repair the epidermis, effectively form a natural protective film, help the balance between the skin and the sebum, can inhibit the peroxidation of skin lipid, can effectively permeate into the skin, promote the proliferation of skin basal cells, and has obvious physiological effects on delaying the skin aging, improving and eliminating chloasma. Squalane can also open skin pores, promote blood microcirculation, promote cell metabolism and help repair damaged cells; the Daphnia lansium fruit oil is grease mainly containing unsaturated fatty acid, wherein the fatty acid contains oleic acid 78.73%, linoleic acid 3.93%, palmitic acid 17.34, and carotenoid, vitamin E and vitamin C, and is used as basic grease of cosmetics, has good permeability, can nourish skin, and the prepared emulsion has good stability and good moisturizing effect.

The invention also provides a preparation method of the essence with the sugar-resisting and oxidation-resisting functions, which comprises the following steps:

(1) adding deionized water, humectant, thickener and antiseptic in weight parts into an emulsifying pot, stirring and heating to 50-65 deg.C, stirring at 200-;

(2) weighing oily components and an emulsifier according to the weight parts, adding into an oil pan, heating to 65-70 ℃, stirring until the oily components and the emulsifier are completely melted, and keeping the temperature for 40min to obtain emulsified oil;

and (3) pouring the emulsified oil obtained in the step (2) into the dispersion liquid obtained in the step (1) under the stirring conditions of 50-65 ℃ and 200-.

According to the technical scheme, compared with the prior art, the essence with the anti-sugar and anti-oxidation functions and the preparation method thereof are provided, multiple functional components are compounded, the skin oxygen free radicals are eliminated, glycosylation is inhibited, oxidation and saccharification of glycosylated protein are treated, the release of inflammatory factors is inhibited, the expression of related genes of barrier repair is promoted, the skin barrier is repaired, aging is delayed, damage is repaired, the fibroblast activity is activated, and the expression of collagen is promoted.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The essence in the examples is prepared by the following method:

Example 1

40 parts of deionized water, 0.1 part of emulsifier, 5 parts of humectant, 0.3 part of antioxidant, 5 parts of anti-glycation agent, 0.1 part of soothing agent, 0.1 part of preservative, 0.1 part of thickening agent and 2 parts of oily component.

The emulsifier is mixture of cetearyl olive oleate and sorbitan olive oleate in any proportion; the humectant is a mixture of butanediol, glycerol polymethacrylate, glycerol polyether-26, isoprene glycol, trehalose, sodium hyaluronate, hydrolyzed hyaluronic acid and arginine in any proportion; the soothing agent is a mixture of panthenol, Agaricus bisporus extract and allantoin at any ratio; the preservative is a mixture of 1, 2-hexanediol, p-hydroxyacetophenone and dipotassium glycyrrhizinate in any proportion; the thickening agent is a mixture of carbomer and xanthan gum in any proportion; the oily component is a mixture of isononyl isononanoate, squalane and palm kernel oil.

The antioxidant is composed of carnosine, inositol, yeast lysate extract, Haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol at a weight ratio of 3:2:1:1:1:1: 1. The anti-glycation agent is decarboxylated carnosine HCl.

Example 2

Example 3

70 parts of deionized water, 3 parts of emulsifier, 20 parts of humectant, 5 parts of antioxidant, 5 parts of anti-glycation agent, 1 part of soothing agent, 1 part of preservative, 1 part of thickening agent and 10 parts of oily component.

Example 4

65 parts of deionized water, 2.1 parts of emulsifier, 16 parts of humectant, 3.8 parts of antioxidant, 4.5 parts of anti-saccharification agent, 0.7 part of soothing agent, 0.3 part of preservative, 0.6 part of thickening agent and 7 parts of oily component.

Example 5

55 parts of deionized water, 1.7 parts of emulsifier, 11 parts of humectant, 3.2 parts of antioxidant, 2.9 parts of anti-saccharification agent, 0.7 part of soothing agent, 0.4 part of preservative, 0.6 part of thickening agent and 5 parts of oily component.

Comparative example 1

The difference from example 2 is that no antioxidant is added.

Comparative example 2

The difference from example 2 is that no anti-glycation agent was added.

Comparative example 3

The difference from example 2 is that the antioxidant is carnosine, yeast lysate extract, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol are mixed in a weight ratio of 3:1:1:1:1: 1.

Comparative example 4

The difference from example 2 is that the antioxidant is inositol, yeast lysate extract, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol are mixed in a weight ratio of 2:1:1:1:1: 1.

Comparative example 5

The difference from example 2 is that the antioxidant is carnosine, inositol, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol are mixed in a weight ratio of 3:2:1:1:1: 1.

Comparative example 6

The difference from example 2 is that the antioxidant is carnosine, inositol, yeast lysate extract, oryzanol and tocopherol mixed in a weight ratio of 3:2:1:1: 1.

Test examples

First, test of oxidation resistance

The products of the above examples and comparative examples were tested for radical scavenging effect by the DPPH method. DPPH is also called 1, 1-diphenyl-2-trinitrophenylhydrazine, has a single electron, has strong absorption at 517nm, and the absolute ethanol solution of DPPH is purple. In the presence of a free radical scavenger, its absorption gradually disappears by pairing with its single electron, and its degree of discoloration is quantitatively related to the number of electrons it receives, so that a rapid quantitative analysis can be performed with a spectrophotometer.

The test method comprises the following steps: DPPH is accurately weighed and prepared into a solution with 0.2mmol/L DPPH, and the solution is stored in a brown bottle in dark at low temperature. And diluting the antioxidant moisturizing composition sample to 1.0mg/mL by using deionized water to obtain a sample solution. Taking 2mL of sample liquid into a test tube, adding 2mL and 0.2mmol/L of DPPH solution, uniformly mixing, standing in a dark place for 30min at room temperature, measuring the absorbance value at 517nm by using an ultraviolet spectrophotometer, and calculating the absorbance value as Ai, simultaneously measuring 2mL of sample liquid and 2mL of absolute ethyl alcohol, standing in a dark place for 30min at room temperature, measuring the absorbance value at 517nm by using an ultraviolet spectrophotometer, and calculating the absorbance value as Aj, and measuring the absorbance value at 517nm by using 2mL of 0.2mmol/LDPPH solution and 2mL of distilled water, standing, and calculating the absorbance value at Ac by using an ultraviolet spectrophotometer. The DPPH radical clearance rate of the sample is calculated according to the following formula: DPPH radical clearance ═ 1- (Ai-Aj)/Ac ] × 100%, the test results are given in the following table:

	DPPH radical scavenging ratio (%)
		Example 1	86.59％
Example 2	89.23％
		Example 3	82.21％
Example 4	81.32％
		Example 5	84.28％
Comparative example 1	62.12％
		Comparative example 2	70.34％
Comparative example 3	74.54％
		Comparative example 4	75.27％
Comparative example 5	78.95％
		Comparative example 6	78.97％

The test results show that the essence prepared by the technical scheme of the invention has a good free radical removal function, namely, the essence has good anti-aging capability. In comparative example 1 and comparative example 2, the antioxidant and the anti-sugar agent were not added, respectively, and the removal effect was poor; in comparative examples 3 to 6, the compositions of the antioxidants are different from those of the present invention, and although the removal effect is due to comparative examples 1 to 2, the requirements are difficult to satisfy due to the lack of the optimal synergistic effect among the components, so that the essence prepared by the formula of the components of the present invention has the optimal antioxidant and saccharification effects, and the optimal synergistic effect among the components.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The essence with the functions of resisting sugar and oxidation is characterized by comprising the following components in parts by weight:

2. The essence with the functions of resisting sugar and resisting oxidation as claimed in claim 1, wherein the emulsifier is a mixture of cetearyl olive oleate and sorbitan olive oleate.

3. The essence with the functions of resisting sugar and resisting oxidation as claimed in claim 1, wherein the humectant is a mixture of butylene glycol, glycerin polymethacrylate, glycerin polyether-26, isoprene glycol, trehalose, sodium hyaluronate, hydrolyzed hyaluronic acid and arginine.

4. The essence with anti-sugar and anti-oxidation functions according to claim 1, wherein the antioxidant is a mixture of carnosine, inositol, yeast lysate extract, haematococcus pluvialis extract, astaxanthin, oryzanol and tocopherol.

5. The essence with the functions of resisting sugar and resisting oxidation as claimed in claim 1, wherein the anti-glycation agent is decarboxylated carnosine HCl.

6. The essence with anti-sugar and anti-oxidation functions as claimed in claim 1, wherein the soothing agent is a mixture of panthenol, agaricus bisporus extract and allantoin.

7. The essence with the functions of resisting sugar and resisting oxidation as claimed in claim 1, wherein the preservative is a mixture of 1, 2-hexanediol, p-hydroxyacetophenone and dipotassium glycyrrhizinate.

8. The essence with the functions of resisting sugar and resisting oxidation as claimed in claim 1 or 2, wherein the thickener is a mixture of carbomer and xanthan gum.

9. The essence with anti-sugar and anti-oxidation functions as claimed in claim 1, wherein the oily component is a mixture of isononyl isononanoate, squalane and palm kernel oil.

10. The preparation method of the essence with the functions of resisting sugar and oxidation as claimed in claims 1 to 9, which is characterized by comprising the following steps:

(3) and (3) pouring the emulsified oil obtained in the step (2) into the dispersion liquid obtained in the step (1) under the stirring conditions of 50-65 ℃ and 200-.