CN113068525A - Scale cutting propagation method for sinojackia xylocarpa - Google Patents

Scale cutting propagation method for sinojackia xylocarpa Download PDF

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CN113068525A
CN113068525A CN202110241574.8A CN202110241574A CN113068525A CN 113068525 A CN113068525 A CN 113068525A CN 202110241574 A CN202110241574 A CN 202110241574A CN 113068525 A CN113068525 A CN 113068525A
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cutting
seedlings
sinojackia xylocarpa
slips
rooting
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李家儒
李海军
魏新增
江明喜
臧润国
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Wuhan University WHU
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings

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  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a large-scale cutting propagation method of sinojackia xylocarpa, belonging to the field of conservation biology. The method comprises the following steps: (1) preparing a cutting seedbed; (2) selecting cutting slips; (3) preparing a rooting agent; (4) cutting; (5) rooting of cutting slips and managing cutting seedlings; (6) and (5) cultivating the big seedlings. Wherein the formula of the rooting agent is 250mg/L alpha-NAA +250mg/L IBA. The temperature and the humidity of the cutting seedbed are independently controlled, the special rooting agent for the sinojackia xylocarpa has good use effect and high rooting rate of cutting slips, the plant type of the large seedlings is regulated and controlled by high density and a tiller inhibitor, the cultivated sinojackia xylocarpa has beautiful large-seedling stem type, can be used for near-field protection, ex-situ protection, natural regression and landscaping of the sinojackia xylocarpa, and has good development and application prospects.

Description

Scale cutting propagation method for sinojackia xylocarpa
Technical Field
The invention belongs to the field of protection biology, and particularly relates to a large-scale cutting propagation method of sinojackia xylocarpa.
Background
Limonium flavum (Sinojackia huangmegensis) is a typical minimal population of wild plants. The establishment of the new technology for the large-scale cutting propagation of the sinojackia xylocarpa is a premise and a foundation for cultivating enough sinojackia xylocarpa seedlings on a large scale and implementing the near-field protection, the ex-situ protection and the natural regression of the sinojackia xylocarpa. At present, in the research literature of the sinojackia xylocarpa, the research on the propagation of the sinojackia xylocarpa, particularly the literature report of the large seedling cultivation is not found.
The cutting propagation technology is widely applied to the production of nursery stocks in forest trees and gardens, but the technical literature reports of the propagation research of the sinojackia xylocarpa are few. The control of the temperature and the humidity of the microenvironment of the cutting seedbed is also the key for determining the success or failure of the cutting. At present, the temperature and the humidity are mainly controlled by full-light spraying in the conventional seedling production, and the effective and independent control of the temperature and the humidity is difficult to realize.
The proper rooting agent is one of the keys for determining the success of the cuttage, and whether the rooting agent is proper or not is related to the specificity of species. The Libra chira, which is a typical wild plant with a very small population, has few research materials and few paradigms of related breeding research results. The common commercial rooting agents such as the commercial ABT rooting powder and the Guoguang rooting agent are applied to the Libra cypress trees for cuttage, and the rooting rate is only 40-60%. Therefore, the research and development of the efficient special rooting agent for the sinojackia xylocarpa suitable for large-scale propagation is very important.
The Libra is white like snow, the fruit is like Libra, the flower and fruit have great ornamental value, but the trunk is not obvious and is in a cluster state, and the plant type characteristics limit the application of the Libra in the garden greening engineering. The method is characterized in that the large seedlings of the sinojackia xylocarpa with prominent trunk and beautiful tree shape are cultured in a large scale, are directly applied to garden greening engineering, are not only the technical key of the sinojackia xylocarpa in large-scale cutting propagation, but also are more active and effective protection for the sinojackia xylocarpa.
The key to the cultivation of the main stem is to inhibit the sprouting of the main stem. In production, the sprout tillers and sprout strips are removed manually, a special sprout tillering inhibitor is developed, the sprout tillering is effectively controlled, and the method has important significance.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a large-scale cutting propagation method of sinojackia xylocarpa.
The purpose of the invention is realized by the following technical scheme:
a scale cutting propagation method of sinojackia xylocarpa comprises the following steps:
(1) cutting seedbed preparation
The upper layer of the seedbed is river sand of 8-10 cm; a small plastic film arched shed is used for covering the seedbed, a sunshade net is covered on the small plastic film arched shed, and the temperature and the humidity of the cuttage seedbed are independently controlled by the small plastic film arched shed and the sunshade net.
The top height of the plastic film small arched shed is preferably 45-60 cm; the sunshade net is preferably supported by an arc steel frame with the top height of 1.8-2.5 m, and the light transmittance of the sunshade net is preferably 30-40%.
(2) Selection of cutting
Selecting tender branches of the annual semi-lignified Huangmei sinojackia xylocarpa from late 6 to early 7 months, and making cutting slips.
The requirements for making the cutting are preferably as follows: the cutting slips are flat at the upper part and inclined at the lower part, the length of the cutting slips is 7-10 cm, the diameter is 0.3-0.4 cm, 4-6 knots are reserved, 1-2 leaves are reserved at the upper end of the cutting slips, and the cutting slips are used at present.
(3) Preparation of rooting agent
Preparing 250mg/L alpha-naphthylacetic acid (alpha-NAA) +250mg/L indolebutyric acid (IBA) rooting agent.
The rooting agent is preferably prepared from the following components in percentage by weight: the alpha-NAA and the IBA are fully dissolved by alcohol and then are added with water to fix the volume.
(4) Cutting
Inserting the base part of the cutting into a rooting agent, soaking for 25-35 seconds, taking out, and directly cutting; spraying carbendazim on the cuttings after cutting, and covering a small plastic film arched shed and a sunshade net.
Cutting requirements: the plant row spacing of the cutting slips is 5 multiplied by 10cm, and the cutting depth is 1/2-2/3 of the length of the cutting slips.
(5) Management of cutting rooting and cutting plantlet
Accurately controlling the temperature and the humidity of the cutting bed after cutting: the humidity of the slotting machine matrix is determined by the degree that the river sand is pinched by hands to be loosened and not agglomerated, and the relative humidity of the air of the slotting machine is kept at 80-90%; the highest temperature in the plastic film small arch shed is not more than 39 ℃. Keeping the temperature and humidity when the cutting seedlings root in the same year, and timely removing weeds on the bed surface to ensure the stable growth environment of the cutting seedlings.
(6) Cultivation of big seedlings
The cutting seedlings are transplanted for the first time in spring next year, and the seedlings with bad plant types need to be stumped. And (4) transplanting the cutting seedlings for two years, and then transplanting for the second time, wherein the height of the seedlings is 1.2-1.5 m and the seedlings are fixed. In the process of culturing the big seedlings, the sprouts of the dry base parts need to be manually removed in time, and sprout inhibitors are sprayed to inhibit the sprouts after the sprouts are removed. The sprout tiller inhibitor is alpha-NAA with the concentration of 200mg/L, and the preparation is preferably as follows: the alpha-NAA is fully dissolved by alcohol and then is added with water to fix the volume. The application of the sprout-tiller inhibitor can effectively control sprout-tiller generation, reduce labor intensity of workers and improve labor productivity.
The plant row spacing for the first transplanting is preferably 0.4 multiplied by 0.5m, and the transplanting density can effectively inhibit the generation and growth of the sprout of the root and neck of the trunk; the row spacing of the plants for the second transplanting is preferably 1.5 multiplied by 1.5 m. The transplanting density is high, so that the occurrence of lateral branches of the sinojackia xylocarpa seedling is inhibited, the development of a straight trunk is promoted, and a beautiful plant type is cultivated.
The invention has the following advantages and beneficial effects: the temperature and the humidity of the cutting seedbed are independently controlled; the rooting agent special for the sinojackia xylocarpa is used, so that the cutting rooting rate is high, and the effect is good; the large seedling type of the cultivated Libra sinojackia xylocarpa is elegant by regulating and controlling the large seedling type through high density and a tiller inhibitor, can be used for near-field protection, ex-situ protection, natural regression and landscaping of the Libra sinojackia xylocarpa, and has good development and application prospects.
Drawings
FIG. 1 is a diagram of the seedling of the Libra yellow plum after cutting and rooting and before transplanting.
FIG. 2 is a drawing of the fixed stem of Libra yellow plum.
Detailed Description
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention.
Example 1
A scale cutting propagation method of sinojackia xylocarpa comprises the following steps:
(1) cutting seedbed preparation
Selecting sandy loam with thick soil layer, loose and fertile soil, good drainage and neutral or slightly acidic soil. The length, the width and the height of the seedbed are 10m multiplied by 1.2m multiplied by 0.25m, the upper layer of the seedbed is river sand of 8-10 cm, and 3% potassium permanganate soil is disinfected (newly mined river sand does not need to be disinfected).
A small plastic film arched shed (with the ceiling height of 50cm) is used for covering the seedbed, a sunshade net (supported by an arc-shaped steel frame with the ceiling height of 2m and with the transmittance of 30-40%) is covered on the small plastic film arched shed, and the temperature and the humidity of the cuttage seedbed are independently controlled by the small plastic film arched shed and the sunshade net.
(2) Selection of cutting
Selecting tender branches of semilignified Huangmeilianzhu trees with no diseases and insect pests and full bud eyes in the current year from the late 6 th to the early 7 th of the month, manufacturing cutting slips, wherein the upper flat mouths and the lower inclined mouths of the cutting slips are 7-10 cm long, 0.3-0.4 cm in diameter and 4-6 knots, and 1-2 leaves are reserved at the upper ends of the cutting slips. The cutting is used as it is.
(3) Preparation of rooting agent
In the step, rooting agents with different formulas are designed for tests, and the results show that after the rooting agent with the formula of 250mg/L alpha-naphthylacetic acid (alpha-NAA) +250mg/L indolebutyric acid (IBA) is used for 6-8 weeks, the cuttage rooting rate is 97%, and the effect is optimal (shown in table 1).
The rooting agent is prepared by taking 1L as a using unit, and is used as the existing rooting agent. Respectively weighing a certain mass of alpha-NAA and IBA, mixing, fully dissolving with a little alcohol, and then metering to 1L with clear water.
TABLE 1 cuttage rooting rate of rooting agent of different concentrations
Figure BDA0002962409200000031
Figure BDA0002962409200000041
(4) Cutting
50-100 cutting slips are bundled into a bundle, and the polarity of the cutting slips is kept consistent during bundling. And (4) inserting the base part of the cutting slip into the prepared rooting agent, soaking for 30 seconds, taking out, and directly cutting. The plant row spacing of the cutting slips is 5 multiplied by 10cm, and the cutting depth is 1/2-2/3 of the length of the cutting slips. And after the cuttage is finished, spraying 500 times of 50% carbendazim solution on the cutting slips.
(5) Management of cutting rooting and cutting plantlet
And 6-8 weeks are needed for rooting after cutting of the cutting slips. The rooting effect of different rooting agents is shown in table 1.
The temperature and the humidity of the cutting bed need to be accurately controlled after cutting. The humidity of the substrate of the slotting machine is controlled by taking the river sand pinched by hand to be loosened and not agglomerated, and the relative humidity of the air of the slotting machine is kept at 80-90%. The highest temperature in the plastic film small arch shed is not more than 39 ℃. Keeping proper temperature and humidity when rooting in the same year, and timely removing weeds on the bed surface to ensure the stable growth environment of the cutting seedlings.
The cutting seedling of the Libra chira after cutting and rooting before transplanting is shown in figure 1.
(6) Cultivation of big seedlings
The cutting seedlings are transplanted in spring next year, the row spacing of the seedlings is 0.4 multiplied by 0.5m, the seedlings with bad plant types need to be stumped, and the transplanting density can effectively inhibit the generation and growth of sprouts at the root neck of the trunk. And after the cuttage seedlings are transplanted for two years, transplanting for the second time is carried out, the plant row spacing is 1.5 multiplied by 1.5m, the seedling height is 1.2m, and the trunk setting seedlings are shown in figure 2. In the process of culturing the big seedlings, the sprouts of the dry base parts need to be manually removed in time, and sprout inhibitors are sprayed to inhibit the sprouts after the sprouts are removed.
The special sprout inhibitor is obtained by design and screening in the step, and the sprout inhibition effect is shown in table 2. The optimal tiller inhibitor is 200mg/L alpha-NAA, which is prepared by the following steps: taking 1L as a using unit, preparing for use on site, weighing 200mg of alpha-NAA, fully dissolving with a little alcohol, and then fixing the volume to 1L with clear water.
TABLE 2 inhibition ratio of different concentrations of sprout-tiller inhibitor to sprout-tiller
Figure BDA0002962409200000051
Example 2
25000 cut seedlings of the sinojackia xylocarpa were cultivated by using the method of example 1 and using the optimal formula of rooting agent and tiller inhibitor. The nursery stocks are distributed to the local places of Huangmei county, and are cultivated in the protection bases of the near places and the migratory places such as Hainan, Yunnan, Guangzhou, Zhejiang, Beijing, tobacco terrace and the like, the survival rate is more than 97 percent, and the nursery stocks have good growth conditions in all places.

Claims (7)

1. A scale cutting propagation method of sinojackia xylocarpa is characterized in that: the method comprises the following steps:
(1) cutting seedbed preparation
The upper layer of the seedbed is river sand of 8-10 cm; covering the seedbed with a small plastic film arched shed, and covering a sunshade net on the small plastic film arched shed;
(2) selection of cutting
Selecting tender branches of the annual semi-lignified Huangmei sinojackia xylocarpa from late 6 to early 7 months, and making cutting slips;
(3) preparation of rooting agent
Preparing a rooting agent of 250mg/L alpha-NAA +250mg/L IBA;
(4) cutting
Inserting the base part of the cutting into a rooting agent, soaking for 25-35 seconds, taking out, and directly cutting; spraying carbendazim on the cuttings after the cutting is finished, and covering a small plastic film arched shed and a sunshade net;
(5) management of cutting rooting and cutting plantlet
Accurately controlling the temperature and the humidity of the cutting bed after cutting: the humidity of the slotting machine matrix is determined by the degree that the river sand is pinched by hands to be loosened and not agglomerated, and the relative humidity of the air of the slotting machine is kept at 80-90%; the highest temperature in the plastic film small arched shed is not more than 39 ℃; keeping the temperature and humidity when the roots grow in the same year, and removing weeds on the bed surface in time;
(6) cultivation of big seedlings
Transplanting the cutting seedlings for the first time in spring next year, and leveling stubbles of the seedlings with poor plant types; transplanting the cutting seedlings for two years, and then transplanting for the second time, wherein the height of the seedlings is 1.2-1.5 m, and the seedlings are fixed dry; in the process of culturing the big seedlings, the sprouts of the dry base parts need to be manually removed in time, and sprout inhibitors are sprayed to inhibit sprouts after the sprouts are removed, wherein the sprout inhibitors are alpha-NAA of 200 mg/L.
2. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (1), the ceiling height of the plastic film small arched shed is 45-60 cm; the sunshade net is supported by an arc steel frame with the top height of 1.8-2.5 m, and the transmittance of the sunshade net is 30-40%.
3. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (2), the requirements for making the cutting slips are as follows: the cutting slips are flat at the upper part and inclined at the lower part, the length of the cutting slips is 7-10 cm, the diameter is 0.3-0.4 cm, 4-6 knots are reserved, 1-2 leaves are reserved at the upper end of the cutting slips, and the cutting slips are used at present.
4. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (3), the rooting agent is prepared by the following steps: the alpha-NAA and the IBA are fully dissolved by alcohol and then are added with water to fix the volume.
5. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (4), the cutting requirements are as follows: the plant row spacing of the cutting slips is 5 multiplied by 10cm, and the cutting depth is 1/2-2/3 of the length of the cutting slips.
6. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (5), the preparation of the tiller inhibitor is as follows: the alpha-NAA is fully dissolved by alcohol and then is added with water to fix the volume.
7. The scale cutting propagation method of the sinojackia xylocarpa according to claim 1, which is characterized in that: in the step (5), the row spacing of the plants transplanted for the first time is 0.4 multiplied by 0.5 m; the row spacing of the plants transplanted for the second time is 1.5 multiplied by 1.5 m.
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JP2015116172A (en) * 2013-12-20 2015-06-25 宮本 忠 Method for cultivating and growing cuttage of willow seedling
CN104041365A (en) * 2014-05-29 2014-09-17 丹阳市保得生态农林开发有限公司 Method for controlling root stalks of date tree to germinate
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Application publication date: 20210706