CN113040158B - Composition and method for preventing and treating branch blight of Korla pear - Google Patents
Composition and method for preventing and treating branch blight of Korla pear Download PDFInfo
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- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a composition for preventing and treating Korla pear branch blight, which comprises a preventing and treating medicament and a plant growth regulator; the control medicament comprises the following components in parts by weight: 5-10 parts of benziothiazolinone, 2-5 parts of shenqinmycin, 1-3 parts of zhongshengmycin, 2-5 parts of a bacillus licheniformis microbial agent and 1-3 parts of a liquid boron fertilizer; the plant growth regulator comprises the following components in parts by weight: 5-8 parts of rhamnolipid, 3-5 parts of chitosan oligosaccharide and 3-6 parts of sugar alcohol calcium. The composition can improve the pesticide effect of the bactericide and obviously reduce the incidence of the Korla fragrant pear branch blight.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a composition for preventing and treating Korla pear branch blight and a prevention and treatment method thereof.
Background
Korla bergamot pear is a special product of the Uygur autonomous region in Xinjiang, is a geographical sign product in China, and is praised as 'pear treasure' and 'fruit prince' due to the characteristics of pleasant color, sweet and smooth taste, rich nutrition and the like. According to statistics, the planting area of the Korla bergamot pears reaches 60 ten thousand mu, the yield value is 20 hundred million yuan, and the method is an important source of income of local fruit growers. However, in recent years, Korla pear has been plagued by a devastating disease, the branch blight disease. The disease is a disease with superimposed bacterial and fungal infectivity, is spread by insect sources, and has great difficulty in prevention and control. The diseases mainly damage the branches and the trunks of fruit trees, and pathogens often invade from the tops of flower organs and twigs and wounds to cause flower rot and branch withering, so that the diseases are fast in onset, fast in spread and high in harmfulness. Branches die, the whole tree dies, and the condition of garden damage brings serious economic loss to fruit growers.
The existing common control method is to chemically control germs, however, the method of spraying bactericide is difficult to achieve better control effect, and effective medicaments for controlling the bacterial wilt are lacking in the market. Patent CN 111034513A discloses a method for preventing and treating Korla pear branch blight, which improves the nutrition supply level of early trees, enhances the stress resistance of the trees and effectively reduces the incidence of the Korla pear branch blight by applying a compound microbial enzyme and a root strengthening nutrient solution. However, the complex microbial ferment still has the disadvantages of complex composition and high cost, and increases the production cost. Therefore, how to provide a composition with low cost and good effect of preventing and treating the Korla pear branch blight is a problem which needs to be solved urgently by researchers.
Disclosure of Invention
The invention provides a composition for preventing and treating Korla pear branch blight and a prevention and treatment method thereof, and aims to solve the technical problem that the prevention and treatment effect of medicaments on the Korla pear branch blight in the current market is poor.
In order to solve the technical problems, the invention provides the following technical scheme:
a composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 5-10 parts of benziothiazolinone, 2-5 parts of shenqinmycin, 1-3 parts of zhongshengmycin, 2-5 parts of a bacillus licheniformis microbial agent and 0.5-2 parts of a liquid boron fertilizer;
the plant growth regulator comprises the following components in parts by weight: 5-8 parts of rhamnolipid, 3-5 parts of chitosan oligosaccharide and 3-6 parts of sugar alcohol calcium.
Preferably, the content of the rhamnolipid is 40-80 g/L.
Preferably, the rhamnolipid is obtained by fermenting and purifying pseudomonas microorganisms.
Preferably, the rhamnolipid simultaneously contains two isomers of disaccharide diglyceride and monosaccharide diglyceride, and the mass content ratio of the disaccharide and the monosaccharide diglyceride is 3-4: 1.
The invention also provides a method for preventing and treating the Korla pear branch blight, which comprises the following steps:
before germination in spring, the control agent is sprayed; spraying the control agent and the plant growth regulator after mixing one week before flowering, one week after flower falling and in a young fruit period; and in the fruit expansion period and the mature period, the plant growth regulator is sprayed.
Preferably, before spring germination, the dilution multiple of the spraying of the control agent is 800-1000 times.
Preferably, the mass ratio of the control agent to the plant growth regulator is 1-2: 1 one week before flowering, and the dilution factor of spraying is 800-1000 times.
Preferably, the mass ratio of the control agent to the plant growth regulator is 1-2: 1 in one week after flower falling and in the young fruit period, and the dilution factor of spraying is 600-800 times.
Preferably, the dilution factor of the spraying of the plant growth regulator is 600-800 times in the fruit expansion period and the mature period.
Preferably, the fruit tree branches, roots and orchard ground are comprehensively sprayed before germination in spring, one week before flowering and one week after flower falling; comprehensively spraying the overground part of the fruit tree in the young fruit period; spraying leaves in the fruit expanding period and the mature period.
Compared with the prior art, the invention has the following beneficial effects:
the invention combines the control agent and the plant growth regulator, thereby not only reducing the dosage and the use frequency of the bactericide, but also improving the pesticide effect, effectively reducing the incidence of the branch blight of the Korla fragrant pears and reducing the risk of orchard damage in orchards.
The benziothiazolinone, shenqinmycin and zhongshengmycin contained in the composition provided by the invention are broad-spectrum efficient bactericides, have low toxicity and low residue, and have better control effects on various bacterial and fungal diseases. The early research results prove that after the three bactericides of benziothiazolinone, shenqinmycin and zhongshengmycin are mixed with the bacillus licheniformis bactericide, the survival rate and the growth of the bacillus licheniformis can not be completely inhibited, the survival rate reaches 86%, the bactericides can be mixed and prepared, and the counteraction effect can not be generated among the bactericides.
In the invention, when the control agent and the plant growth regulator are used together, the final control effect on the Korla pear branch blight is 82.86%, which is obviously higher than the control effects of respectively using the plant growth regulator and the control agent alone.
Detailed Description
A composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 5-10 parts of benziothiazolinone, 2-5 parts of shenqinmycin, 1-3 parts of zhongshengmycin, 2-5 parts of a bacillus licheniformis microbial agent and 0.5-2 parts of a liquid boron fertilizer;
the plant growth regulator comprises the following components in parts by weight: 5-8 parts of rhamnolipid, 3-5 parts of chitosan oligosaccharide and 3-6 parts of sugar alcohol calcium.
In the composition for preventing and treating the branch blight of the Korla fragrant pears, the benziothiazolinone is added, is a novel broad-spectrum bactericide, and is mainly used for preventing and treating various bacterial and fungal diseases such as cucumber downy mildew, pear scab, apple scab, citrus anthracnose, grape anthracnose and the like. The sterilization mechanism mainly comprises two aspects of destroying the nuclear structure of pathogenic bacteria, leading the pathogenic bacteria to lose heart parts and die due to failure, interfering the metabolism of pathogenic bacteria cells, leading the pathogenic bacteria cells to be disordered physiologically and finally leading the pathogenic bacteria cells to die. In the present invention, the amount of benziothiazolinone is 5 to 10 parts by weight, preferably 6 to 8 parts by weight, and more preferably 7 parts by weight. The source of the benziothiazolinone is not particularly limited in the present invention, and a commercially available product can be used.
The composition for preventing and treating the bacterial wilt of the Korla pear is added with the shenqinmycin, which is an antibiotic secreted by pseudomonas fluorescens M18 through biological culture, and simultaneously has a bactericide with double functions of inhibiting plant pathogenic bacteria in a broad spectrum and promoting plant growth, has the characteristics of broad spectrum and high efficiency, and can effectively prevent and treat blight, gummy stem blight, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, stripe rust, sclerotinia, gibberellic disease, anthracnose, gray mold, scab, leaf spot, bacterial wilt, ulcer disease, ginger disease and soil-borne disease on crops such as rice, wheat, vegetables and the like. In the invention, the amount of the shenqinmycin is 2-5 parts by weight, preferably 3-4 parts by weight. The source of the shenqinmycin is not specially limited, and a commercial product is adopted.
The composition for preventing and treating the branch blight of the Korla pear is added with the medium-level mildewcide, which is a novel agricultural antibiotic developed successfully by biocontrol of Chinese academy of agricultural sciences, is an antibiotic produced by Hainan variety of Streptomyces lavendulae and belongs to N-glycoside alkaline water-soluble substances. The medium-level mycophenolic acid has high activity on bacterial diseases and partial fungal diseases of crops, and has a certain yield increasing effect. In the invention, the addition amount of the medium-sized mildewcide is 1 to 3 parts by weight, and preferably 2 parts by weight. The source of the zhongshengmycin is not specially limited, and a commercially available product is adopted.
The composition for preventing and treating the bacterial wilt of the Korla pear is added with the bacillus licheniformis microbial agent, the bacillus licheniformis is common gram-positive bacteria in soil, can generate a plurality of antibacterial active substances such as lichenin and the like, has a unique biological oxygen-deprivation action mechanism, and inhibits the growth and the propagation of pathogenic bacteria. In the invention, the addition amount of the bacillus licheniformis agent is 2-5 parts by weight, preferably 3 parts by weight. The source of the bacillus licheniformis agent is not specially limited, and a commercially available product is adopted.
The branch blight is a disease caused by various actions of pathogenic bacteria, fungi and the like, and the current practice proves that the branch blight can not be prevented and controlled by singly using any one of the fungicide of benziothiazolinone, shenqinmycin, zhongshengmycin and bacillus licheniformis. The early research results prove that after the three bactericides of benziothiazolinone, shenqinmycin and zhongshengmycin are mixed with the bacillus licheniformis bactericide, the survival and the growth of the bacillus licheniformis can not be completely inhibited, and the survival rate reaches 86 percent, so the bactericides can be mixed and prepared. Compared with the single use of each medicament, the compounded medicament has a synergistic effect, can effectively prevent and treat the Kurla pear deadwood disease, and reduces the incidence rate of the Kurla pear deadwood disease.
The liquid boron fertilizer is added into the composition for preventing and treating the Korla pear branch blight, boron is one of essential nutrient elements of plants, plays an important role in synthesis and transportation of a product of photosynthesis, namely carbohydrate, and has a special role in normal fertilization. The application of the boric fertilizer can improve the stress resistance of plants, thereby better resisting the invasion of pathogenic bacteria, and the boric fertilizer is compounded with benziothiazolinone, shenqinmycin, zhongshengmycin and bacillus licheniformis microbial inoculum to prepare a control medicament, thereby effectively improving the control effect of the Kuerle fragrant pear fusarium wilt. In the invention, the addition amount of the liquid boron fertilizer is 0.5-2 parts by weight, preferably 1 part by weight. The source of the liquid boron fertilizer is not specially limited, and the liquid boron fertilizer can be obtained by adopting a commercial product.
In the composition for preventing and treating the Korla pear branch blight, the rhamnolipid is a biological surfactant metabolite generated by microbial metabolism, has the effects of reducing the surface/interface tension of liquid, solubilizing, emulsifying, spreading and other auxiliary agents, is green, safe and residue-free, has properties superior to those of a chemical surfactant, and does not have a series of environmental pollution problems caused by the chemical surfactant, and more importantly, the structure of the rhamnolipid has similarity with the lipid bilayer structure of a plant cell membrane, so that a special 'pore channel' can be formed on the surface of the cell membrane to promote the penetration of liquid medicine, and the pesticide effect is improved. The reasonable addition of the rhamnolipid is an important condition that the preventive and therapeutic agent can play a role in preventing and treating diseases. According to the difference of fermentation strains and fermentation processes, a plurality of isomer structures exist, and more or less functional differences exist among different structures. The more common rhamnolipids have four structures: Rha-Rha-C10-C10(R1 disaccharide diester), Rha-C10-C10(R2 monosaccharide diester), Rha-Rha-C10(R3 disaccharide monoester), and Rha-C10(R4 monosaccharide monoester). The mono-glycolipid has higher ability to lower interfacial tension than the di-glycolipid, which is superior in emulsifying and penetrating cell membrane. In the invention, the addition amount of the rhamnolipid is 5-8 parts by weight, preferably 6-7 parts by weight. In the invention, the rhamnolipid is obtained by fermenting and purifying pseudomonas microorganisms, preferably, the rhamnolipid simultaneously contains two isomers of disaccharide biolipid and monosaccharide biolipid, and the mass content ratio of the disaccharide biolipid to the monosaccharide biolipid is 3-4: 1, preferably 3: 1. In the invention, the content of the rhamnolipid is 40-80g/L, and preferably 60 g/L.
The composition for preventing and treating the Korla pear branch blight is added with chitosan oligosaccharide, also called chitosan oligosaccharide, which is known as beta-1, 4-oligo-glucosamine, and chitosan is used as a raw material, and is degraded by a biotechnology to form a low-molecular-weight product with good water solubility, large functional effect and high biological activity. The chitosan oligosaccharide can effectively improve the yield of fruits and vegetables, prevent and treat plant diseases and insect pests, and proliferate beneficial bacteria of soil and biological bacterial manure. The chitosan oligosaccharide has the special double-effect of the pesticide and fertilizer, so that the chitosan oligosaccharide is widely applied to the agricultural field. The chitosan oligosaccharide added in the invention provides necessary nutritional conditions for the growth of crops, and improves the capability of preventing and controlling the Korla fragrant pear branch blight. In the invention, the addition amount of the chitosan oligosaccharide is 3-5 parts by weight, preferably 4 parts by weight. The source of the chitosan oligosaccharide is not particularly limited, and the chitosan oligosaccharide can be obtained by adopting a commercially available product.
The sugar alcohol calcium is added into the composition for preventing and treating the Korla pear branch blight, so that the absorption rate of a calcium fertilizer is greatly improved, calcium elements are supplemented in time, the calcium deficiency symptom of crops is corrected, and necessary nutritional conditions are provided for the growth of the crops. According to the invention, the sugar alcohol calcium increases the disease resistance and appearance quality of crops, improves the using effect of the pesticide, and improves the capability of preventing and controlling the Korla fragrant pear branch blight. In the invention, the sugar alcohol calcium is added in an amount of 3-6 parts by weight, preferably 4-5 parts by weight. The source of sugar alcohol calcium in the invention is not particularly limited, and a commercially available product can be adopted.
The invention provides a method for preventing and treating branch blight of Korla fragrant pears, which comprises the following steps:
before germination in spring, the control agent is sprayed; spraying the control agent and the plant growth regulator after mixing one week before flowering, one week after flower falling and in a young fruit period; and in the fruit expansion period and the mature period, the plant growth regulator is sprayed.
In the method for preventing and treating the branch blight of the Korla fragrant pear, the preferable application mode is water-added spraying; the spraying standard is a conventional standard; in the embodiment of the invention, the type of the device preferably used is a knapsack sprayer, and the diameter of the spraying hole is 0.8 mm.
In the method for preventing and treating the branch blight of the Korla fragrant pears, the prevention and treatment agent is preferably diluted to be 1000 times of 800-fold for spraying before the germination in spring. Preferably, the spraying is carried out on the branches, the roots and the ground of the fruit trees comprehensively.
In the method for controlling the branch blight of the Korla fragrant pear, the control agent and the plant growth regulator are preferably mixed in a mass ratio of 1-2: 1, preferably 1: 1, mixing, wherein the dilution multiple of spraying is 800-1000 times. Preferably, the spraying is carried out on the branches, the roots and the ground of the fruit trees comprehensively.
In the method for preventing and treating the branch blight of the Korla fragrant pear, the control agent and the plant growth regulator are preferably mixed in a mass ratio of 1-2: 1 in one week after flower falling and in a young fruit period, and preferably in a ratio of 1: 1, mixing, wherein the dilution multiple of spraying is 600-800 times. Preferably, the fruit tree branches, roots and orchard ground are comprehensively sprayed one week after flower falling; and in the young fruit period, the overground part of the fruit tree is sprayed comprehensively.
In the method for preventing and treating the branch blight of the Korla fragrant pears, the plant growth regulator is preferably diluted to be 800 times of 600-fold and sprayed in the fruit expansion period and the mature period. Foliar spraying is preferably carried out.
The present invention will be described in detail with reference to examples for better understanding the objects, technical solutions and advantages of the present invention, but they should not be construed as limiting the scope of the present invention.
Example 1
A composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 5 parts of benziothiazolinone, 2 parts of shenqinmycin, 1 part of zhongshengmycin, 2 parts of a bacillus licheniformis microbial agent and 0.5 part of a liquid boric fertilizer;
the plant growth regulator comprises the following components in parts by weight: 5 parts of rhamnolipid, 3 parts of chitosan oligosaccharide and 3 parts of sugar alcohol calcium.
Example 2
A composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 10 parts of benziothiazolinone, 5 parts of shenqinmycin, 3 parts of zhongshengmycin, 5 parts of a bacillus licheniformis microbial agent and 2 parts of a liquid boric fertilizer;
the plant growth regulator comprises the following components in parts by weight: 8 parts of rhamnolipid, 5 parts of chitosan oligosaccharide and 6 parts of sugar alcohol calcium.
Example 3
A composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 6.5 parts of benziothiazolinone, 3 parts of shenqinmycin, 2 parts of zhongshengmycin, 4 parts of a bacillus licheniformis microbial agent and 1 part of a liquid boric fertilizer;
the plant growth regulator comprises the following components in parts by weight: 6 parts of rhamnolipid, 4.5 parts of chitosan oligosaccharide and 4 parts of sugar alcohol calcium.
Example 4
A composition for preventing and treating Korla pear branch blight comprises a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 8 parts of benziothiazolinone, 4 parts of shenqinmycin, 2 parts of zhongshengmycin, 4 parts of a bacillus licheniformis microbial agent and 1.5 parts of a liquid boric fertilizer;
the plant growth regulator comprises the following components in parts by weight: 7 parts of rhamnolipid, 4 parts of chitosan oligosaccharide and 5 parts of sugar alcohol calcium.
Example 5
The composition of example 3 was used as a test agent;
conditions of the experiment
The Korla pear trees in the test park are 9 years old, and the occurrence of the branch blight is serious. And performing a field plot bacterial wilt prevention and control test in a composition mode in 2018. The test period is from 4 months and 5 days to 9 months and 10 days, and covers the whole growth period of the pear trees. Each treatment was repeated 3 times, with 5 pear trees in each repeat. And (4) investigating 20 new branches of each pear tree, calculating the diseased branch rate of each branch, and calculating the control effect of each treatment.
The formula for calculating the diseased branch rate and the control effect is as follows:
TABLE 1 design of field drug effect test
The application method comprises the following steps: adding water for spraying; the spraying standard is a conventional standard; the type of the used instrument is a knapsack sprayer, and the diameter of a spraying hole is 0.8 mm.
Data requirements of other pest control agents
During the test period, except for spraying the test agent, the test agent is not sprayed to any other pesticide in each cell.
The test agent is safe and has no phytotoxicity to the tested pear varieties.
Investigating the prevention effect once in the middle of 5 months, 6 months and 8 months respectively, and counting the diseased branch rate and the prevention effect of the branch blight of each cell.
Table 2 field control of the branch blight of Korla pear by each treatment group
The field test results show that the final control effect on the Korla fragrant pear branch blight is 40.35% when only the control agent and no plant growth regulator are used in the treatment (II). And (3) the treatment (III) only uses the plant growth regulator and does not use a control agent, and the final control effect is 33.74 percent. The final control effect of the control agent and the plant growth regulator on the Korla pear branch blight in the treatment (I) is 82.86 percent and is 42.51 percent and 49.12 percent higher than that in the treatment (II) and the treatment (III) respectively. The combined use has better disease control effect. The control effect on the branch blight by the treatment (II) and the treatment (III) is not more than 50 percent, which indicates that the control effect on the branch blight of the Korla pear cannot be achieved by using any kind of medicament alone. The combined use of the two medicaments achieves 82.86% of the control effect on the Korla pear, which shows that the two medicaments have obvious enhancement effect.
Example 6
Test agent
Treatment group (one) agent composition:
the control medicament comprises the following components in parts by weight: 5 parts of benziothiazolinone, 2 parts of shenqinmycin, 1 part of zhongshengmycin, 2 parts of a bacillus licheniformis microbial agent and 0.5 part of a liquid boric fertilizer; the plant growth regulator comprises the following components in parts by weight: 5 parts of rhamnolipid, 3 parts of chitosan oligosaccharide and 3 parts of sugar alcohol calcium.
Treatment group (two) composition of agents:
compared with the treatment group (I), the treatment group (I) does not contain benziothiazolinone and a bacillus licheniformis microbial agent.
Treatment group (iii) agent composition:
rhamnolipids were not added compared to treatment group (one).
Treatment group (four) agent composition:
compared with the treatment group (I), the treatment group (I) does not contain benziothiazolinone, a bacillus licheniformis microbial agent and rhamnolipid.
Conditions of the experiment
The Korla pear trees in the test park are 8 years old, and the occurrence of the branch blight is serious. And performing a field plot bacterial wilt prevention and control test in a composition mode in 2017. The test period is from 4 months and 8 days to 9 months and 10 days, and covers the whole growth period of the pear trees. Each treatment was repeated 3 times, with 5 pear trees in each repeat. And (4) investigating 20 new branches of each pear tree, calculating the diseased branch rate of each branch, and calculating the control effect of each treatment.
The formula for calculating the diseased branch rate and the control effect is as follows:
TABLE 3 design of field drug effect test
The application method comprises the following steps: adding water for spraying; the spraying standard is a conventional standard; the type of the used instrument is a knapsack sprayer, and the diameter of a spraying hole is 0.8 mm.
Data requirements of other pest control agents
During the test period, except for spraying the test agent, the test agent is not sprayed to any other pesticide in each cell.
The test agent is safe and has no phytotoxicity to the tested pear varieties.
Investigating the prevention effect once in the middle of 5 months, 6 months and 8 months respectively, and counting the diseased branch rate and the prevention effect of the branch blight of each cell.
Table 4 shows the field control effect of the treatment groups on the branch blight of Korla fragrant pears
The field test results show that the final control effect on the Korla pear branch blight after the treatment of the control agent lacking the benziothiazolinone and the bacillus licheniformis microbial agent and the normally prepared plant growth regulator is 39.12%. And (3) the plant growth regulator lacking rhamnolipid is treated, and the final control effect on the Korla pear branch blight is 38.35% when the plant growth regulator is used together with a normally prepared control agent. And (IV) the control agent lacking the benziothiazolinone and the bacillus licheniformis microbial inoculum and the plant growth regulator lacking the rhamnolipid are combined to be used, and the final control effect on the bacterial wilt of the Korla pear is 29.32%. The final control effect of the normally prepared agent on the Korla pear branch blight reaches 86.24%, which is far higher than that of the other three groups of agent treatment groups. The three substances play a vital role in controlling diseases, but the single use of the substances cannot play an effective disease control effect. Therefore, the composition has positive significance for preventing and treating the Korla pear branch blight.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A composition for preventing and treating Korla pear branch blight is characterized by comprising a preventing and treating agent and a plant growth regulator;
the control medicament comprises the following components in parts by weight: 5-10 parts of benziothiazolinone, 2-5 parts of shenqinmycin, 1-3 parts of zhongshengmycin, 2-5 parts of a bacillus licheniformis microbial agent and 0.5-2 parts of a liquid boron fertilizer;
the plant growth regulator comprises the following components in parts by weight: 5-8 parts of rhamnolipid, 3-5 parts of chitosan oligosaccharide and 3-6 parts of sugar alcohol calcium.
2. The composition for controlling the Korla pear branch blight disease according to claim 1, wherein the content of the rhamnolipid is 40-80 g/L.
3. The composition for controlling the Korla pear branch blight disease according to claim 1, wherein the rhamnolipid is obtained by fermenting and purifying a microorganism belonging to the genus Pseudomonas.
4. The composition for preventing and treating the Korla pear branch blight disease as claimed in claim 1, wherein the rhamnolipid contains two isomers of disaccharide diester and monosaccharide diester at the same time, and the mass content ratio of the disaccharide diester to the monosaccharide diester is 3-4: 1.
5. A method for preventing and treating branch blight of Korla fragrant pears is characterized by comprising the following steps:
before spring germination, spraying the control agent of claim 1; spraying the controlling agent of claim 1 after mixing with the plant growth regulator of claim 1 one week before flowering, one week after flower fall, and at a young fruit stage; spraying the plant growth regulator of claim 1 during the fruit expansion period and the mature period.
6. The prevention and treatment method as claimed in claim 5, wherein the dilution factor for spraying the prevention and treatment agent is 800-1000 times before spring germination.
7. The control method as claimed in claim 5, wherein the mass ratio of the control agent to the plant growth regulator is 1-2: 1 and the dilution factor for spraying is 800-1000 one week before flowering.
8. The control method according to claim 5, characterized in that the mass ratio of the control agent to the plant growth regulator is 1-2: 1 at one week after flower drop and at the young fruit stage, and the dilution factor of spraying is 600-800 times.
9. The method as claimed in claim 5, wherein the dilution factor for spraying the plant growth regulator is 600-800 times in the fruit expansion period and the mature period.
10. The control method according to claim 5, characterized in that the fruit tree branches, roots and orchard ground are sprayed all over before germination in spring, one week before flowering and one week after flower falling; comprehensively spraying the overground part of the fruit tree in the young fruit period; spraying leaves in the fruit expanding period and the mature period.
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| 《苹果细菌性枯枝病菌的生物学特性及抑菌药剂筛选》;李燕等;《中国农学通报》;20210205;全文 * |
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