CN113040000B - Phellinus igniarius cultivation method capable of achieving fast Phellinus igniarius emergence - Google Patents
Phellinus igniarius cultivation method capable of achieving fast Phellinus igniarius emergence Download PDFInfo
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Abstract
The invention discloses a phellinus igniarius cultivation method with fast phellinus igniarius emergence, which comprises stock seed preparation, cultivated seed preparation and phellinus igniarius cultivation.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a phellinus igniarius cultivation method capable of achieving rapid phellinus igniarius emergence.
Background
Phellinus igniarius is a rare medicinal fungus, is named as 'forest gold' in great name, has been used for more than 2000 years in China, is recorded in detail in the herbal works of the past generations about the medicinal function of Phellinus igniarius, and has the main functions of treating dysentery, night sweat, metrorrhagia, bloody stranguria, umbilical and abdominal pain, rectocele and bleeding, leukorrhagia, amenorrhea, diarrhea stopping diarrhea, prolonging life and the like. Modern researches show that the ganoderma lucidum polysaccharide has obvious effects of resisting tumor, resisting oxidation, enhancing immunity, preventing and treating rheumatoid arthritis, reducing blood fat, inhibiting uric acid and the like, has high medicinal value, is proved to be superior to medicinal fungi such as ganoderma lucidum and the like particularly in the aspect of resisting tumor, becomes a hotspot for research and development of domestic and foreign medicinal preparations and health care products, and has good economic benefit and social benefit
The existing phellinus igniarius cultivation method is slow and unstable in phellinus igniarius emergence, long in production period and low in biological efficiency, so that how to develop a phellinus igniarius cultivation method which is fast in phellinus igniarius emergence, capable of stabilizing the phellinus igniarius, stable in growth and development of sporocarp, small in yield and quality fluctuation, short in production period, low in management cost and high in biological efficiency is a technical problem to be solved.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The phellinus linteus sanghuang poison vanninii QJF-3 is preserved in China general microbiological culture Collection center at 23.07.23.2020, with the address of No. 3 Siro-1 of Beijing republic of Chaoyang, and the preservation number of CGMCC No.20227 at the institute of microbiology of China academy of sciences.
As one aspect of the present invention, the present invention provides a method for cultivating Phellinus linteus with rapid emergence of Phellinus linteus, comprising the steps of,
(1) mixing 75-80% of miscellaneous wood chips, 13-18% of wheat bran, 1-3% of bean pulp, 1-2.5% of sugar and 1-1.5% of quick lime according to weight percentage, fully stirring uniformly, controlling the water content to be 62-65%, filling into a polypropylene fungus bag with the folding diameter of 17cm, and keeping for 2 hours at 121 ℃;
(2) placing the sterilized fungus bags into a cooling chamber to be cooled to room temperature, inoculating the cultivars into a culture material according to the proportion of 5 percent by weight under the aseptic condition, covering the cultivars with a material surface of more than 1/2, immediately sealing after inoculation, and culturing in a dark room with good ventilation condition at the temperature of 22-28 ℃ until hyphae grow over the fungus bags;
(3) transferring the fungus bags with the surfaces full of light yellow mycelia into a cultivation shed, stacking the fungus bags together, covering a sunshade net on the surfaces, and giving scattered light in the shed to finish color conversion of the fungus bags and achieve physiological maturity;
(4) digging a pit in the shed, spraying chlorothalonil in the pit, cutting a cut on a physiologically mature fungus bag by using a sterilization blade, spraying a microbial agent to the cut, sleeving the bag, vertically placing the bag on a fungus bed, and stimulating the growth of yellow by adopting a day and night temperature difference method, namely the day temperature is 25 ℃, the night temperature is 18 ℃, the air humidity is 85-90% during bud forcing, and scattering light;
(5) and after the cut is yellow, removing the bag, spraying water, increasing the air humidity to 90-95%, increasing the ventilation, illuminating by 800lux, and harvesting when the sporocarp tissue is hard, the pileus is turned from bright yellow to dark yellow and a small amount of spores are ejected.
The cultivation method is a bag cultivation mode.
As a preferable scheme of the phellinus igniarius cultivation method with fast yellow color emergence: the microbial agent comprises a composite probiotic fermentation product, chitosan, trace elements, polypeptide and selenium element; the probiotic-containing composite fermented food comprises, by mass, 8-15 parts of the composite probiotic fermented product, 1-5 parts of chitosan, 0.5-1 part of trace elements, 0.1-1 part of polypeptide and 5-8 parts of selenium elements.
As a preferable scheme of the phellinus igniarius cultivation method with fast yellow color emergence: the microbial agent also comprises a disease-resistant growth promoting factor.
As a preferable scheme of the phellinus igniarius cultivation method with rapid phellinus igniarius emergence: the microbial agent is characterized in that the compound probiotic fermentation product comprises fermentation products of rhodopseudomonas palustris, bacillus subtilis and bacillus mucilaginosus.
As a preferable scheme of the phellinus igniarius cultivation method with fast yellow color emergence: the preparation method of the composite probiotic fermentation product comprises the steps of respectively activating rhodopseudomonas palustris, bacillus subtilis and bacillus mucilaginosus according to a conventional method, mixing, inoculating the activated rhodopseudomonas palustris, bacillus subtilis and bacillus mucilaginosus into a liquid fermentation culture medium according to the mass percentage of 3%, and culturing for 48-72 hours at the temperature of 30 ℃.
The Phellinus linteus comprises QJF-3 of Phellinus linteus with preservation number of CGMCC No. 20227.
As a preferable scheme of the phellinus igniarius cultivation method with fast yellow color emergence: the formula of the microbial agent is as follows: the probiotic compound fermented product comprises, by weight, 10 parts of composite probiotic fermented product, 3 parts of chitosan (with a molecular weight of 50kDa), 7 parts of sodium selenite, 0.8 part of potassium dihydrogen phosphate and 0.2 part of soybean polypeptide; the preparation method of the composite probiotic fermentation product comprises the steps of respectively activating rhodopseudomonas palustris (Rhodopseudomonas aeruginosa, ACCC 10650), Bacillus subtilis (Bacillus subtilis var. aterlimus, GDMCC 800060) and Bacillus mucilaginosus (Bacillus mucilaginosus, ACCC 10012) according to a conventional method, mixing the activated Bacillus subtilis and the activated Bacillus mucilaginosus according to a mass ratio of 1:2:1, and inoculating the activated Bacillus mucilaginosus to a liquid fermentation culture medium according to a mass percentage of 3%.
As a preferable scheme of the phellinus igniarius cultivation method with fast yellow color emergence: the miscellaneous wood chips in the step (1) are mulberry branch wood chips or oak wood chips and oak wood chips.
As another aspect of the present invention, there is provided a method for cultivating Phellinus linteus with rapid yellowing, comprising the steps of,
(1) chopping oak cut in winter in the current year into blocks, splicing into section wood bundles with the diameter of 15-17 cm, bundling the section wood bundles by using a plastic rope, soaking the section wood bundles in clear water for 20min, fishing out the wood bundles, controlling water for 20min, filling low-pressure polyethylene material bags with the folding diameter of 19-22 cm, the length of 39-45 cm and the thickness of 0.005-0.008 cm into the low-pressure polyethylene material bags, filling sawdust nutrient materials into two ends and gaps of a wood section, wherein the ratio of sawdust to wheat bran is 3:1, adding appropriate amount of water for uniformly stirring, controlling the water content to be 55-66%, bundling bag openings, tightly attaching the section wood and the plastic bags, sterilizing at normal pressure, controlling the temperature to rise to 100 ℃ within 4-6 h, keeping the temperature for 16-18 h, and taking the cut section wood out of the pot when the temperature naturally falls below 60 ℃;
(2) adopting a one-end inoculation mode, digging strains by using an inoculation shovel, quickly feeding the strains into a material bag, covering the strains on a material surface above 1/2, immediately sealing after inoculation, and culturing in a dark room with good ventilation conditions at 22-28 ℃ until hyphae grow to fill the material bag;
(3) transferring the fungus sticks with the surfaces full of the faint yellow mycelia into a cultivation shed, stacking the fungus sticks together, covering a sunshade net on the surfaces, and giving scattered light in the shed to enable the fungus bags to finish color conversion and achieve physiological maturity;
(3) digging pits in the shed according to the depth of 6cm and the distance between the plants and the rows of 10cm or 12cm, and spraying the chlorothalonil in the pits; cutting a cut on a physiologically mature fungus bag by using a sterilization blade, vertically placing the fungus bag in a pit, burying sandy soil, spraying a microbial agent to the cut of the fungus stick, sleeving the fungus bag, vertically placing the fungus bag on a fungus bed, and stimulating the fungus stick to be yellow by adopting a day and night temperature difference method, namely 25 ℃ in the daytime, 18 ℃ at night, 85-90% of air humidity during bud forcing and scattering light;
after the cut part turns yellow, the sleeved bag is removed, the water is sprayed, the air humidity is improved to 90 percent, the ventilation is increased, and the illumination is 800 lux; after the greenhouse is in winter, directly covering the sun-shading net on the surface of the fungus stick, controlling the temperature and keeping the moisture, and uncovering the sun-shading net to the top of the greenhouse in the next 4 months to play a sun-shading role; after the local air temperature is 20 ℃, the phellinus igniarius sporocarp enters a growing period and is managed in a conventional fruiting mode.
The cultivation method is a wild-imitating cultivation mode.
The invention has the beneficial effects that: the phellinus igniarius cultivation method provided by the invention has the advantages of high fungus growing speed, high yellow color generation speed, stable yellow color generation, stable growth and development of sporocarp, low fluctuation of yield and quality, short production period, low management cost and high biological efficiency.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1: morphological characteristics and classification identification of phellinus igniarius strain QJF-3
(1) Morphological characteristics of Phellinus linteus strain QJF-3: the second and third layers of the sporocarp are stacked, the sporocarp is in a horseshoe shape or a fan shape, the back surface of the pileus is provided with an obvious yellow wide band, the ventral surface is yellow, and the texture is soft; culturing in a culture dish (PDA culture medium) for 14d, wherein the colony diameter reaches 90mm, and the texture is villiform, yellow and brown on the back; the hypha is light brown, multi-branched and provided with a diaphragm under the observation of a microscope, the diameter is 2.5-4.5 colors, and the morphological characteristics show that the hypha is authentic phellinus igniarius.
(2) ITS identification of Phellinus linteus strain QJF-3
Extracting DNA of phellinus igniarius strain QJF-3 by a conventional method, and performing PCR amplification by using ITS1 and ITS4 as primers, wherein the primer sequences of ITS1 and ITS4 are as follows:
ITS1:5'--3'(SEQ ID No.1)-TCCGTAGGTGAACCTGCGG
ITS4:5'--3'(SEQ ID No.2)-TCCTCCGCTTATTGATATGC
the PCR amplification system is as follows: premix 12.5TG, ITS1 and ITS4 each 1. mu.L, DNA template 2. mu.L, using ddH2O was supplemented to 25. mu.L.
Amplification conditions: pre-denaturation at 95 ℃ for 5 min; denaturation at 94 ℃ for 3min, annealing at 55 ℃ for 50s, and extension at 72 ℃ for 45s for 33 cycles; fully extend for 7min at 72 ℃.
Detecting the PCR product by 1.2% agarose gel electrophoresis, wherein the target fragment is about 700bp, sending the PCR product to Shanghai Biotech company for sequencing, and the sequence information is as follows:
5'-AACGAGCTTTGAGCGAGACTTGCTGCTGGCGCGAATGAATTTTTGCGCATGTGCACGGCCTTCGCGCTCAAATCCAACTCTCAAACCCCCTGTGCACCTTATTATATCGCGAGTCGAAGTTAGTAGTCTGAGGTCTGTCTTGTAAGTAATGAGTAGAAGGGCGAAAGCGAGCGAGTGTTGCTCGTTAGGTAACCCTTCGAAAATGAAAGCGAGCGGGAGTGCGTCGGGTGAAGACTTGGGCTTGTTGTTACAAACCCCCCCCTTATATTGTCTTTGTGAATGTAACGCTCCTCGTGGGCGAAAATCAATACAACTTTCAACAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATGTTTATCTCAAACCGCTCGTCTTTCTTTAATTGAAGGGCTTGAGGTTTGGACTTGGAGGTTTACTGCTGGCCGCCTTTCGAAGGGGGTCGGCTCCTCTTAAATACATTAGCTGGGCTTTGGCTCGCGCTTACGGTGTAATAGTTGATTCCGTTCACCAACGAGCGCTTGCCTAACGAGCTTGCTTCTAACGGTCCGCTTGGTCGGACAAGGAGTTGTTGTTACCTCCTTTTTCTTGACACCTTGACCTCAAATCAGGGTACGA-3'
the sequences were Blast aligned to show the highest homology to the ITS sequence of Sanghuang lung vanil i.
According to the comprehensive analysis of the experimental data of the sporophore morphology, the mycelium morphology, the microscopic characteristics, the ITS identification and the like of the strain, the phellinus igniarius strain is proved to be phellinus igniarius, and the Latin name of the phellinus igniarius strain is Sanghuangporus vanninii.
Example 2: cultivation experiment of Phellinus linteus QJF-3
(1) Preparing stock seeds: mixing 81% of sawdust (half of coarse sawdust and half of fine sawdust), 15% of wheat bran, 2% of bean cake, 0.5% of lime, white sugar and 1% of gypsum according to the weight percentage, adding water, fully and uniformly stirring, controlling the water content to be between 62% and 65%, bagging, controlling the specification of a cultivation bag to be 17cm, controlling the specification of the cultivation bag to be uniform, controlling the water content of a polypropylene bag, tying a rubber band, sterilizing at high pressure, and keeping at 125 ℃ for 120 min. And (3) after the fungus bags are sterilized, taking out and spreading for cooling, inoculating the phellinus igniarius QJF-3 test tube seeds under the aseptic condition, and culturing for 40 days under the conditions of 28 ℃ and light protection to obtain phellinus igniarius QJF-3 stock seeds.
(2) Preparation of cultivars: the composition and preparation method of the culture material of the cultivated species are shown in step (1), the stock seeds obtained in step (1) are inoculated on the culture material of the cultivated species according to the proportion of 5 percent by weight, and the cultivated species are cultivated for 40 days under the condition of 28 ℃ and dark place, so that the cultivated species of phellinus igniarius QJF-3 are obtained.
(3) Bag cultivation method of phellinus igniarius QJF-3
Mixing 78% of mixed wood chips, 18% of wheat bran, 2% of bean pulp, 1% of sugar and 1% of quicklime according to the weight percentage, adding water, fully and uniformly stirring, controlling the water content to be 62-65%, filling into a polypropylene fungus bag with the diameter of 17cm, and keeping the temperature at 121 ℃ for 2 hours;
placing the sterilized fungus bags into a cooling chamber to be cooled to room temperature, inoculating the cultivated species in the step (2) into a culture material according to the proportion of 5 percent by weight under the aseptic condition, covering the cultivated species with a material surface of more than 1/2, immediately sealing the inoculated cultivated species by using a bacterium filtering sealing film or a jacket bag, and culturing the inoculated cultivated species at 28 ℃ in a dark room with good ventilation condition until hypha grows over the fungus bags;
before the fungus sticks enter a cultivation shed, digging pits according to the depth of 3-4 cm and the distance of 10cm or 12cm between plant rows, spraying chlorothalonil in the pits, moving the fungus sticks with faint yellow mycelia on the surface into the cultivation shed, cutting openings (crescent) on the fungus bags by using a sterilization blade, standing upside down in the pits, burying wet sandy soil, dividing the cultivation shed into A, B areas, spraying a microbial agent to the cutting openings of the phellinus fungus sticks in the B area, bagging, vertically placing on a fungus bed, and taking the A area as a contrast without any treatment. Then the yellow color is stimulated by adopting a day and night temperature difference method, namely the temperature in the day is about 25 ℃, and the temperature at night is about 18 ℃. In the bud forcing period, the air humidity is 85%, the cut is scattered light, the cut in the area B is basically yellow, the fungus sticks in the area A are yellow, and most of the fungus sticks are not yellow.
After the cut part turns yellow, the bag is removed, the water is sprayed, the air humidity is increased to 90%, the ventilation is increased, the illumination is 800lux, the growth period of the sporocarp is increased, the growth vigor of the sporocarp of the fungus stick in the B area is better than that of the fungus stick in the A area, the agronomic characters are all better, and the fungus can be harvested after the fungus tissue is hard and the fungus cover is changed from bright yellow to dark yellow and a small amount of spores are ejected.
The formula of the microbial agent is as follows: the probiotic compound fermented product comprises, by weight, 10 parts of composite probiotic fermented product, 3 parts of chitosan (with a molecular weight of 50kDa), 7 parts of sodium selenite, 0.8 part of potassium dihydrogen phosphate and 0.2 part of soybean polypeptide; the preparation method of the composite probiotic fermentation product comprises the steps of respectively activating rhodopseudomonas palustris (ACCC 10650), Bacillus subtilis var. aterlimus (GDMCC 800060) and Bacillus mucilaginosus (ACCC 10012) according to a conventional method, mixing the activated Bacillus subtilis var. aterlimus and the activated Bacillus mucilaginosus according to a mass ratio of 1:2:1, inoculating the activated Bacillus mucilaginosus and the activated Bacillus mucilaginosus into a liquid fermentation culture medium according to a mass percentage of 3%, wherein the liquid fermentation culture medium comprises 1% of peptone, 2% of beef extract, 2% of glucose, 1% of yeast extract, 0.1% of ammonium citrate, 0.05% of magnesium sulfate and the balance of distilled water, and culturing the mixture at 30 ℃ for 72 hours to enable the strain concentration to reach 108And (5) cfu/mL or more, so as to obtain the composite probiotic fermentation product. When in use, the water is diluted and sprayed according to the ratio of 1: 200.
TABLE 1
(4) Wild-imitating cultivation method of phellinus igniarius QJF-3
Chopping oak felled in winter in the current year into blocks, splicing into section wood bundles (3 kg-4.5 kg) with the diameter of 15 cm-17 cm, bundling the section wood bundles by using a plastic rope, soaking the section wood bundles in clear water for 20min, fishing out, controlling water for 20min, filling low-pressure polyethylene material bags with the folding diameter of 19 cm-22 cm, the length of 39 cm-45 cm and the thickness of 0.005 cm-0.008 cm, filling sawdust and wheat bran nutrition materials (the ratio of sawdust to wheat bran is 3: 1) into two ends and gaps of a wood section, adding proper amount of water for uniformly stirring, controlling the water content to be 55% -66%, bundling the section wood and the plastic bag, tightly attaching the section wood and the plastic bag, sterilizing at normal pressure, controlling the temperature to rise to 100 ℃ within 4 h-6 h, keeping for 16 h-18 h at the temperature, and taking the section wood out of the plastic bag after the temperature in the pot naturally falls below 60 ℃.
Adopting a mode of inoculating at one end, digging strains by an inoculating shovel, quickly feeding the strains into a material bag, covering the strain surface above 1/2, immediately sealing by a strain sealing film or a jacket bag after inoculating, and culturing in a dark room at 28 ℃ under good ventilation conditions until hypha grows over the strain bag.
Before the fungus sticks enter the cultivation shed, digging pits according to the depth of 6cm and the distance of 10cm or 12cm between plant rows, and spraying the chlorothalonil in the pits; the method comprises the steps of moving a fungus stick with a surface full of faint yellow mycelia into a cultivation shed, cutting a cut (cutting a fungus skin) on the fungus bag by using a sterilization blade, vertically placing the fungus stick in a pit, burying sandy soil, wherein the water content of the sandy soil is 70% -72%, dividing the cultivation shed into A, B two areas, spraying a microbial agent to the cut of the phellinus igniarius fungus stick in the B area, bagging, vertically placing the fungus stick on a fungus bed, and taking the A area without any treatment as a comparison. Then the yellow color is stimulated by adopting a day and night temperature difference method, namely the temperature in the day is about 25 ℃, and the temperature at night is about 18 ℃. In the bud forcing period, the air humidity is 85% -90%, the light is scattered, the cut part is observed within 10 days, the cut part in the area B is basically yellow, fungus sticks in the area A are yellow, and most of the fungus sticks are not yellow.
After the cut part turns yellow, the sleeved bag is removed, the water is sprayed, the air humidity is increased to 90%, the ventilation is increased, the illumination is 800lux, the growth period of the fruiting body is longer than that of the fruiting body in the region A, the growth vigor of the fruiting body of the fungus stick in the region B is better than that of the fruiting body in the region A, and the agronomic characters are better.
After the greenhouse is in winter, the sun-shading net is directly covered on the surface of the fungus stick, the temperature is controlled, the moisture is preserved, and the sun-shading net is uncovered to the top of the greenhouse in the next 4 months of the year, so that the sun-shading effect is achieved. After the local air temperature is 20 ℃, the phellinus igniarius sporocarp enters a growing period and is managed in a conventional fruiting mode.
Three-year-old phellinus igniarius is stacked in two layers or three layers, is in a horseshoe shape or a fan shape, is woody and hard, and can be harvested when pileus is dark yellow to brown, white growth rings on the edge disappear and a small amount of spores are ejected.
TABLE 2
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (3)
1. A method for cultivating phellinus igniarius with fast yellow color generation is characterized in that: comprises the following steps of (a) carrying out,
(1) mixing 75-80% of miscellaneous wood chips, 13-18% of wheat bran, 1-3% of bean pulp, 1-2.5% of sugar and 1-1.5% of quick lime according to weight percentage, fully and uniformly stirring, controlling the water content to be 62-65%, filling into a polypropylene fungus bag with the folding diameter of 17cm, and keeping for 2 hours at 121 ℃;
(2) placing the sterilized fungus bags into a cooling room to be cooled to room temperature, inoculating the cultivated species into a culture material according to the proportion of 5 percent by weight under the aseptic condition, covering the cultivated species with a material surface above 1/2, immediately sealing after inoculation, and culturing in a dark room with good ventilation condition at the temperature of 22-28 ℃ until hyphae grow to fill the fungus bags;
(3) transferring the fungus bags with the surfaces full of faint yellow mycelia into a cultivation shed, stacking the fungus bags together, covering the surface with a sunshade net, and giving scattered light in the shed to enable the fungus bags to finish color conversion and achieve physiological maturity;
(4) digging a pit in the shed, spraying chlorothalonil in the pit, cutting a cut on a physiologically mature fungus bag by using a sterilization blade, spraying a microbial agent to the cut, sleeving the bag, vertically placing the bag on a fungus bed, and stimulating the yellow color by adopting a day and night temperature difference method, namely the day temperature is 25 ℃, the night temperature is 18 ℃, the air humidity is 85-90%, and scattering light;
(5) after the cut part turns yellow, removing the bagged fungus, spraying water, increasing the air humidity to 90-95%, increasing the ventilation, illuminating by 800lux, and collecting when the fruiting body tissue becomes hard, the pileus turns from bright yellow to dark yellow and a small amount of spores are ejected;
the cultivation method is a bag cultivation mode; the microbial agent comprises, by weight, 10 parts of composite probiotic fermentation product, 3 parts of chitosan, 7 parts of sodium selenite, 0.8 part of potassium dihydrogen phosphate and 0.2 part of soybean polypeptide; the molecular weight of the chitosan is 50 kDa; the composite probiotic fermentation product consists of fermentation products of rhodopseudomonas palustris, bacillus subtilis and bacillus mucilaginosus; the preparation method of the composite probiotic fermentation product comprises the following steps: activating rhodopseudomonas palustris, bacillus subtilis and bacillus mucilaginosus respectively according to a conventional method, mixing the activated rhodopseudomonas palustris, the bacillus subtilis and the bacillus mucilaginosus according to the mass ratio of 1:2:1, inoculating the activated rhodopseudomonas palustris, the bacillus subtilis and the bacillus mucilaginosus into a liquid fermentation culture medium according to the mass percentage of 3%, and culturing the activated rhodopseudomonas palustris, the bacillus subtilis and the bacillus mucilaginosus for 48 to 72 hours at the temperature of 30 ℃;
the phellinus igniarius is QJF-3, and the preservation number is CGMCC No. 20227.
2. The method for cultivating Phellinus linteus with rapid yellowing according to claim 1, wherein: the microbial agent also comprises a disease-resistant growth promoting factor.
3. The method for cultivating Phellinus linteus with rapid yellowing according to claim 1, wherein: the miscellaneous wood chips in the step (1) are mulberry branch wood chips or oak wood chips and oak wood chips.
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| CN107484544A (en) * | 2017-07-04 | 2017-12-19 | 徐州山野菌业有限公司 | A kind of cultural method of pleurotus eryngii |
| CN107996288B (en) * | 2017-12-29 | 2020-11-10 | 浙江省林业科学研究院 | Method for ecologically cultivating phellinus igniarius under forest by imitating wild conditions |
| CN110089341A (en) * | 2018-01-31 | 2019-08-06 | 广西宜州天源绿佳农业科技有限公司 | A kind of cultural method of ramulus mori Pleurotus citrinopileatus |
| CN109258293B (en) * | 2018-11-27 | 2020-09-18 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | High-yield cultivation method for morchella |
| CN110301283A (en) * | 2019-06-10 | 2019-10-08 | 江苏康能生物工程股份有限公司 | A kind of Phellinus industrialization segment wood cultivated method |
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