Micro-operation device capable of realizing accurate control of liquid suction and discharge
Technical Field
The invention relates to the technical field of micromanipulation, in particular to a micromanipulation device capable of realizing accurate control of liquid suction and discharge.
Background
The microscope is an instrument for observing microscopic objects invisible to naked eyes and is a mark for human beings to enter atomic times; microscopy is the science of using this instrument to study microscopic objects and structures; "microscopic" means invisible to the naked eye unless observed by a microscope; the microscope is an optical instrument formed by one lens or a combination of several lenses, and the microscopes are of various types and mainly divided into an optical microscope and an electron microscope; optical microscopy, the most common microscope and the earliest microscope invented, uses light to create an image through a sample; in the operation of a microscope, the most initial and important step is to extract a sample, and no object exists without the observation of a sample microscope; especially in the case of liquid samples, the extraction process is more complicated.
At present, the prior micro-operation on liquid mostly can not carry out relatively accurate suction and discharge treatment on the liquid; the low precision of the microscopic operation may result in too much or too little liquid suction; if too much liquid is sucked, resources are wasted, and if too little liquid is sucked, inaccurate experimental results caused by too little sample can be caused; similarly, if the discharged liquid cannot be accurately controlled, the experimental result is easily influenced; in addition, the prior pipette and the prior drain pipe are integrated with the microscope, so that the prior pipette and the prior drain pipe are difficult to disassemble and carry out handheld operation; therefore, a new apparatus is required to be put into use to solve the above problems.
Disclosure of Invention
The present invention is to provide a micromanipulation apparatus capable of controlling the suction and discharge of liquid accurately, so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a micromanipulation device capable of realizing accurate control of liquid suction and discharge comprises a workbench, a bracket, a lens barrel, an ocular and a rotating shaft; the support is fixedly arranged at the top of the rear side of the workbench, a connecting shaft is arranged on the support, and the outer sides of the left side wall and the right side wall of the support are rotatably connected with a support frame through the connecting shaft; a lens barrel is arranged in the support frame in a sliding mode, and the top of the lens barrel is connected with an eyepiece through threads; the left side and the right side of the bottom of the lens cone are respectively connected with a liquid discharge pipe and a liquid suction pipe; rubber pipes are fixedly arranged at the bottoms of the liquid suction pipe and the liquid discharge pipe; a fixing plate is fixedly arranged at the inner bottom of the liquid suction pipe, and the top of the fixing plate is connected with a rotating plate through a rotating shaft; the top parts of the rotating plate and the fixed plate are both provided with vertically upward imbibition conduits, and the top parts of the imbibition conduits are connected with drainage plates fixedly arranged in the imbibition pipes; the top of the rotating plate is fixedly provided with a connecting plate, the top of the connecting plate is rotatably connected with a vertically upward transmission shaft, and the top of the transmission shaft extends out of the top of the liquid suction pipe and is rotatably connected with a first knob; a continuous liquid level sensor is arranged above the drainage plate on the inner wall of the liquid suction pipe; the outer wall of the right side of the liquid suction pipe is sequentially and fixedly provided with an air exhaust device and a communicating pipe communicated with the liquid discharge pipe from top to bottom; and a valve is arranged at the top of the communicating pipe.
Protective housings which are mutually communicated are arranged on the left side and the right side of the liquid discharge pipe, a screw rod is rotatably connected between the upper sides of the protective housings on the left side and the right side, the right side of the screw rod extends out of the right wall of the protective housing and is rotatably connected with a second knob, and flow control plates are rotatably connected on the screw rods in the protective housings on the two sides; a shaft sleeve is arranged on the internal screw rod of the liquid discharge pipe; and a liquid storage tank with a circular through hole is arranged at the center of the bottom of the liquid discharge pipe.
As a further scheme of the invention: the liquid discharge pipe and the liquid suction pipe are connected with the lens barrel in a threaded manner.
As a further scheme of the invention: the fixing plate is provided with a plurality of conduit holes arranged along circles with different diameters.
As a further scheme of the invention: the diameter of the rotating plate is smaller than that of the fixing plate, and a plurality of guide pipe holes are formed in the circular surface along circles with different diameters.
As a further scheme of the invention: the drainage plate is in a structure with high edge, middle and low edge, and a drainage groove leading to the outside of the pipette is formed in the center of the drainage plate.
As a further scheme of the invention: both sides of the screw rod are provided with threads in different directions.
As a further scheme of the invention: the shape of the flow control plate is arc-shaped.
As a still further scheme of the invention: the bottom material of the liquid storage tank is an electromagnet; and the top of the through hole of the liquid storage tank is provided with an iron ball with the diameter larger than that of the through hole.
Compared with the prior art, the invention has the beneficial effects that: in the device, the connection modes of the liquid suction pipe and the liquid discharge pipe with the platform are threaded, so that the liquid suction pipe and the liquid discharge pipe can be detached and independently operated by hands; the disassembled pipette can be used as a liquid discharge pipe at the same time; the liquid level change in the device can be monitored in real time through the continuous liquid level sensor and the display; the liquid suction pipe is provided with the fixed plate, the rotating plate and the communicating pipes, and the design mode can control the number of the communicating pipes between the rotating plate and the conduit holes on the fixed plate and change the diameter of the communicating pipes through rotating the rotating plate so as to achieve the effect of accurately controlling the liquid level; the device is provided with a drainage plate structure with high edge and low center, and the arrangement of the drainage plate can effectively avoid the backflow of liquid; the liquid discharge pipe in the device is internally provided with a flow control plate with controllable spacing, and the flow control plate can change the flowing speed of liquid in the liquid discharge pipe so as to preliminarily control the discharge of fluid; in addition, the liquid discharging precision is controlled by utilizing the repulsive force between the electromagnet and the iron ball in the liquid discharging pipe, the design mode is simple in structure, and the control effect is good.
Drawings
FIG. 1 is a schematic perspective view of a micromanipulation apparatus capable of precisely controlling the intake and discharge of a liquid.
FIG. 2 is a schematic cross-sectional view of a pipette in a micromanipulation apparatus capable of performing precise controlled aspiration and discharge of a liquid.
FIG. 3 is a schematic cross-sectional view of a drain tube in a micromanipulation device capable of achieving precise controlled aspiration and expulsion of fluid.
FIG. 4 is a schematic structural diagram of a fixing plate of a micromanipulation apparatus capable of accurately controlling the suction and discharge of a liquid.
FIG. 5 is a schematic diagram of a rotating plate in a micromanipulation device that can achieve precise controlled aspiration and expulsion of liquid.
Wherein: 1. a work table; 2. a support; 3. a connecting shaft; 4. a lens barrel; 5. an eyepiece; 6. a support frame; 7. a pipette; 8. a liquid discharge pipe; 9. a continuous liquid level sensor; 10. a first knob; 11. a drive shaft; 12. an air extraction device; 13. a valve; 14. a communicating pipe; 15. a connecting plate; 16. a fixing plate; 17. a rubber tube; 18. a rotating shaft; 19. a rotating plate; 20. a liquid suction conduit; 21. a drainage plate; 22. a flow control plate; 23. an iron ball; 24. a liquid storage tank; 25. a protective shell; 26. a second knob; 27. a shaft sleeve; 28. a screw; 29. a conduit aperture; 30. a display.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Referring to fig. 1-5, a micromanipulation apparatus capable of precisely controlling suction and discharge of liquid includes a worktable 1, a support 2, a lens barrel 4, an eyepiece 5, a rotating shaft 18 and a display 30; the support 2 is fixedly arranged at the top of the rear side of the workbench 1, a connecting shaft 3 is arranged on the support 2, and the outer sides of the left side wall and the right side wall of the support 2 are rotatably connected with a support frame 6 through the connecting shaft 3; a lens barrel 4 is arranged in the support frame 6 in a sliding mode, and the top of the lens barrel 4 is connected with an eyepiece 5 in a threaded mode; the left side and the right side of the bottom of the lens cone 4 are respectively in threaded connection with a liquid discharge pipe 8 and a liquid suction pipe 7, and the liquid discharge pipe 8 and the liquid suction pipe 7 can be detached and independently operated by hands; the pipette 7 after disassembly can have both the functions of aspirating and discharging liquid; rubber pipes 17 are fixedly arranged at the bottoms of the liquid suction pipe 7 and the liquid discharge pipe 8; a fixing plate 16 is fixedly installed at the inner bottom of the pipette 7, a plurality of conduit holes 29 arranged along circles with different diameters are formed in the fixing plate 16, and a rotating plate which is smaller in diameter than the fixing plate 16 and is provided with a plurality of conduit holes 29 along circles with different diameters is connected to the top of the fixing plate 16 through a rotating shaft 18; the top parts of the rotating plate 29 and the fixed plate 16 are both provided with a vertically upward liquid suction conduit 20, the top part of the liquid suction conduit 20 is connected with a drainage plate 21 which is fixedly arranged in the liquid suction pipe 7 and has a shape of high edge and low middle, and the drainage plate 21 is designed to avoid the situation of liquid backflow; a drainage groove leading to the outside of the pipette 7 is formed in the center of the drainage plate 21; the top of the rotating plate 19 is fixedly provided with a connecting plate 15, the top of the connecting plate 15 is rotatably connected with a vertical upward transmission shaft 11, and the top of the transmission shaft 11 extends out of the top of the pipette 7 and is rotatably connected with a first knob 10; the first knob 10 is rotated, the first knob 10 drives the transmission shaft 11 to start rotating, the rotation of the transmission shaft 11 further drives the rotating plate 19 to rotate through the rotation of the connecting plate 15, the rotation of the rotating plate 19 can control the communication number of the rotating plate 19 and the conduit holes 29 on the fixed plate 16, and further the liquid suction can be preliminarily controlled; a continuous liquid level sensor 9 is arranged above the drainage plate 21 on the inner wall of the pipette 7, and the continuous liquid level sensor 9 can continuously monitor the change of the liquid level; the liquid level is convenient to control; an air exhaust device 12 and a communicating pipe 14 communicated with the liquid discharge pipe 8 are sequentially and fixedly arranged on the outer wall of the right side of the liquid suction pipe 7 from top to bottom; the suction means 12 can control the amount of liquid sucked in by controlling the pressure inside the pipette 7; the top of the communicating pipe 14 is provided with a valve 13, and the valve 13 can further control the liquid flow.
Example two
On the basis of the first embodiment, in order to optimize the performance of the device; protective shells 25 which are mutually communicated are arranged on the left side and the right side of the liquid discharge pipe 8, screw rods 28 with threads in different directions on two sides are rotatably connected between the upper sides of the protective shells 25 on the left side and the right side, the right sides of the screw rods 28 extend out of the right wall of the protective shells 25 and are rotatably connected with a knob II 26, and circular arc-shaped flow control plates 22 are rotatably connected on the screw rods 28 in the protective shells 25 on the two sides; rotating the second knob 26, wherein the screw 28 is driven to rotate by the rotation of the second knob 26, and the rotation of the screw 28 can further control the flow control plates 22 on the two sides to approach or separate from each other, so that the outflow speed of the liquid can be preliminarily controlled; a shaft sleeve 27 is arranged on a screw 28 in the liquid discharge pipe 8, and the screw 28 can be protected by the shaft sleeve 27; a liquid storage tank 24 with a circular through hole at the center is arranged at the bottom of the liquid discharge pipe 8, and the bottom of the liquid storage tank 24 is made of an electromagnet; an iron ball 23 with the diameter larger than that of the through hole is arranged at the top of the through hole of the liquid storage tank 24; the device can control the suspension height of the iron ball 23 by changing the magnetism of the electromagnet, thereby further controlling the discharge of liquid; the display 30 is fixedly arranged on the side surface of the bracket 2, and the display 30 can display the liquid level real-time data in the device in real time.
The working principle of the invention is as follows: in pipetting, firstly starting the air suction device 12, wherein the air suction device 12 can control the amount of the sucked liquid by controlling the pressure inside the pipette 7; then, the first knob 10 is rotated, the first knob 10 drives the transmission shaft 11 to start to rotate, the rotation of the transmission shaft 11 further drives the rotation plate 19 to rotate through the rotation of the connecting plate 15, the rotation of the rotation plate 19 can control the communication number of the rotation plate 19 and the conduit holes 29 on the fixing plate 16, and further the liquid suction can be preliminarily controlled; the continuous liquid level sensor 9 can continuously monitor the change of the liquid level; the liquid level is convenient to control; a valve 13 is arranged at the top of the communicating pipe 14, and the valve 13 can further control the liquid flow; when liquid is drained, the second knob 26 is rotated, the screw 28 is driven to rotate by the rotation of the second knob 26, and the rotation of the screw 28 can further control the flow control plates 22 on the two sides to approach or separate from each other, so that the outflow speed of liquid can be preliminarily controlled; finally, the suspension height of the iron ball 23 can be controlled by changing the magnetism of the electromagnet, and further the discharge of the liquid can be controlled.
Although the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.