CN102719355A - Cell vacuum absorption fixing device - Google Patents
Cell vacuum absorption fixing device Download PDFInfo
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- CN102719355A CN102719355A CN2012102505868A CN201210250586A CN102719355A CN 102719355 A CN102719355 A CN 102719355A CN 2012102505868 A CN2012102505868 A CN 2012102505868A CN 201210250586 A CN201210250586 A CN 201210250586A CN 102719355 A CN102719355 A CN 102719355A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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Abstract
The invention discloses a cell vacuum absorption fixing device comprising a comprehensive bottom plate, an ovum functional plate and a first sperm tank, wherein the ovum functional plate is provided with a concave ovum tank; the side of the comprehensive bottom plate is provided with a connector; and the ovum functional plate is also provided with a plurality of micropores formed in the bottom of the ovum tank. The cell vacuum absorption fixing device also comprises a pressure control loop comprising a diaphragm liquid pump, a pressure sensor, an electro-hydraulic servo valve and a liquid discharging pipe, wherein an inlet of the diaphragm liquid pump is connected with the electro-hydraulic servo valve, and an outlet of the diaphragm liquid pump is connected with the liquid discharging pipe; and the input end of the pressure sensor is connected between the connector and the electro-hydraulic servo valve, and the output end of the pressure sensor is connected to the input end of the electro-hydraulic servo valve. Cells are automatically fixed and released by means of the work of the diaphragm liquid pump, and the diaphragm liquid pump has the characteristics of high efficiency, high repeatability and the like.
Description
Technical field
The present invention relates to field of medical, relate in particular to a kind of cell vacuum suck stationary installation.
Background technology
Micromanipulative technique (Micromanipulation Technique) is meant under the high power inverted microscope; Utilize micromanipulator (Micromanipulator); The mechanism that control microinjection pin moves in field of microscope, a kind of method that is used for carrying out cell or body early embryo operation.The material that can be injected into cell includes: various organoids, kinases, histological chemistry's mark (such as horseradish peroxidase or fluorescent yellow), protein, metabolic substd, little magnetic head, ion, antibody, gene, molecular biological mRNA and DNA or the like.Use this technology, also can realize being used for a small amount of (skin the rises to milliliter) medicament of individual cells or one group of cell or the accurate conveying (slight irrigation notes) of medicine.
In modern times in the fields such as biology, medical science, agricultural; Injection cell has obtained application more and more widely; But experiment whole efficiency and success ratio are all not high; Influence factor comprises by the operation links such as control of injection cell seizure with fixing, accurately location, cytomorphosis and the fragmentation of microinjection pin, microinjection amount; Chinese scholars is demarcated and is detected from cell search and location, the fast feed of microinjection pin, the control of microinjection amount, image recognition processing, micro-operation locus respectively, improved the level of automation of injection cell in various degree, but the integrated automation level is also very low.The operation of injection cell at present need be asked and at first use the sticking pin with cell capture in addition; Accomplish injection process with entry needle then, so usually system needs two operators, and cell all need be used sticking pin absorption, fixing; Efficiency ratio is lower; And in injection process, cell flows easily, influences the success ratio of injection cell.Therefore study new cellular localization and stationary installation, microinjection pin fast feed method, the accurate control of microinjection amount and automatic cytological injection operation technology and system architecture etc. are significant to realization injection cell high success rate, high-throughput.
Existing injection cell system all is based on the structure of tradition " the simple eye formula of both hands "; The operator observes through microscope ocular and finds the object run cell; Utilize the left and right sides operator that is installed on the microscope both sides then, wherein left hand is the sticking pin, is used for catching of cell; The right hand is an entry needle, accomplishes the injection of cell.Can know by the injection cell characteristics; The cell that is in micron dimension is suspended in nutrient solution usually, and the sticking pin moves in nutrient solution or slight vibrations, causes the nutrient solution disturbance easily and drives cell position and change; Cause very difficulty of operations such as cell search and location; Stability is not enough, owing to can only fix a cell at every turn, causes the injection cell conventional efficient low in addition.Simultaneously, operator must have very strong professional technique technical ability, need through long-term strict training.The microinjection system that present biotechnology circle generally uses is basically by Japanese Narishige, and German Eppendorf and U.S. Sutter three companies capture, and existing injection cell system still is in the manual or semi-automatic stage.Therefore study the automatic identification of cell and the new device of fixed cell, change the injection system of traditional left and right sides operator, and then improve the core technology that injection cell efficient is popularization injection cell system applies.
Therefore, to above-mentioned technical problem, be necessary to provide a kind of batch cell vacuum suck stationary installation, to overcome above-mentioned defective.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of cell vacuum suck stationary installation, this cell vacuum suck stationary installation has high precision, characteristics such as high-level efficiency and high repeatability.
For realizing above-mentioned purpose, the present invention provides following technical scheme:
A kind of cell vacuum suck stationary installation; It comprises comprehensive egative film, be installed on ovum functional sheet on the comprehensive egative film, be formed at the first sperm groove on the comprehensive egative film; Said ovum functional sheet is provided with the ovum groove of spill, and the side of said comprehensive egative film is provided with a joint, also is provided with the some micropores that are formed at the ovum trench bottom on the said ovum functional sheet; Said cell vacuum suck stationary installation also comprises pressure control circuit; Said pressure control circuit comprises diaphragm type liquid pump, pressure transmitter, the electrohydraulic servo valve that is connected with joint and liquid discharge pipe, and the import of said diaphragm type liquid pump is connected with electrohydraulic servo valve, and the outlet of said diaphragm type liquid pump is connected with liquid discharge pipe; The input terminus of said pressure transmitter is connected between joint and the electrohydraulic servo valve, and the output terminal of said pressure transmitter is connected to the input terminus of electrohydraulic servo valve.
Preferably; In above-mentioned cell vacuum suck stationary installation; Said comprehensive egative film be provided with depression in order to first rectangular tank that the ovum functional sheet is installed and be positioned at first rectangular tank and further second rectangular tank of depression; Said second rectangular tank is a vacuum chamber, is provided with the passage that makes liquid communication between said vacuum chamber and the joint.
Preferably, in above-mentioned cell vacuum suck stationary installation, said cell vacuum suck stationary installation also comprises the raceway groove that is formed at the second sperm groove on the comprehensive egative film and connects the first sperm groove and the second sperm groove.
Preferably, in above-mentioned cell vacuum suck stationary installation, the width range of said raceway groove is 80 ~ 120 μ m.
Preferably, in above-mentioned cell vacuum suck stationary installation, the diameter range of said micropore is 10 ~ 500 μ m.
Preferably, in above-mentioned cell vacuum suck stationary installation, said joint is connected to electrohydraulic servo valve by silicone tube, and the other end of said electrohydraulic servo valve is connected to the import of diaphragm type liquid pump by silicone tube.
Preferably, in above-mentioned cell vacuum suck stationary installation, said ovum groove is square.
Can find out that from technique scheme the cell vacuum suck stationary installation of the embodiment of the invention relies on the work of diaphragm type liquid pump to fix or discharges cell, and is repeatable high; And device can carry out the fixing of batch cell, high efficiency; The output terminal of said pressure transmitter is received the input terminus of electrohydraulic servo valve, forms closed loop, accurately controls the pressure of liquid in the pipeline.
Description of drawings
In order to be illustrated more clearly in the embodiment of the invention or technical scheme of the prior art; To do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art below; Obviously, the accompanying drawing relevant of the present invention in describing below only is some embodiments of the present invention, for those of ordinary skills; Under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the schematic perspective view of cell vacuum suck stationary installation of the present invention;
Fig. 2 is the schematic diagram of pressure control circuit in the cell vacuum suck stationary installation of the present invention;
Fig. 3 is the enlarged view of micropore on the ovum functional sheet in the cell vacuum suck stationary installation of the present invention;
Fig. 4 is the enlarged view of raceway groove between two sperm grooves in the cell vacuum suck stationary installation of the present invention.
Wherein: 1, comprehensive egative film; 2, ovum functional sheet; 3, ovum groove; 4, micropore; 5, the first sperm groove; 6, raceway groove; 7, the second sperm groove; 8, vacuum chamber; 9, joint; 10, diaphragm type liquid pump; 11, electrohydraulic servo valve; 12, pressure transmitter; 13, liquid discharge pipe.
Embodiment
The invention discloses a kind of cell vacuum suck stationary installation, this cell vacuum suck stationary installation has high precision, characteristics such as high-level efficiency and high repeatability.
This cell vacuum suck stationary installation comprises comprehensive egative film, be installed on ovum functional sheet on the comprehensive egative film, be formed at the first sperm groove on the comprehensive egative film.Said ovum functional sheet is provided with the ovum groove of spill.The side of said comprehensive egative film is provided with a joint.Also be provided with the some micropores that are formed at the ovum trench bottom on the said ovum functional sheet.Said cell vacuum suck stationary installation also comprises pressure control circuit, and said pressure control circuit comprises diaphragm type liquid pump, pressure transmitter, the electrohydraulic servo valve that is connected with joint and liquid discharge pipe.The import of said diaphragm type liquid pump is connected with electrohydraulic servo valve; The outlet of said diaphragm type liquid pump is connected with liquid discharge pipe; The input terminus of said pressure transmitter is connected between joint and the electrohydraulic servo valve, and the output terminal of said pressure transmitter is connected to the input terminus of electrohydraulic servo valve.
Further; Said comprehensive egative film be provided with depression in order to first rectangular tank that the ovum functional sheet is installed and be positioned at first rectangular tank and further second rectangular tank of depression; Said second rectangular tank is a vacuum chamber, is provided with the passage that makes liquid communication between said vacuum chamber and the joint.
Further, said cell vacuum suck stationary installation also comprises the raceway groove that is formed at the second sperm groove on the comprehensive egative film and connects the first sperm groove and the second sperm groove.
Further, the width range of said raceway groove is 80 ~ 120 μ m.
Further, the diameter range of said micropore is 10 ~ 500 μ m.
Further, said joint is connected to electrohydraulic servo valve by silicone tube, and the other end of said electrohydraulic servo valve is connected to the import of diaphragm type liquid pump by silicone tube.
Further, said ovum groove is square.
To combine the accompanying drawing in the embodiment of the invention below, the technical scheme in the embodiment of the invention is carried out detailed description, obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on the embodiment among the present invention, the every other embodiment that those of ordinary skills are obtained under the prerequisite of not making creative work belongs to the scope that the present invention protects.
To shown in Figure 3, cell vacuum suck stationary installation comprises comprehensive egative film 1, be installed on ovum functional sheet 2 on the comprehensive egative film 1, be formed at the first sperm groove 5 on the comprehensive egative film 1 like Fig. 1.The size of comprehensive egative film 1 is according to general slide glass, and only thickness increases a little, is applicable to most microscopes.Ovum functional sheet 2 is provided with ovum groove 3 spill and that be square.Ovum functional sheet 2 and comprehensive egative film 1 all are be that raw material is injection molded with medical polycarbonate (PC), and nontoxic, light transmission is good, suitable micrurgy.The side of comprehensive egative film 1 is provided with a joint 9, also is provided with the some micropores 4 that are formed at ovum groove 3 bottoms on the ovum functional sheet 2, and the diameter range of micropore 4 is 10 ~ 500 μ m.Micropore 4 can break into laser-beam drilling machine, so is provided with, and is convenient to process the different through hole of diameter, and then can operate to different ovum.In the cell vacuum suck stationary installation of the embodiment of the invention, can be as required, do the quantity of portalling and be: 1,2,3 * 3 arrays, 4 * 4 arrays, 5 * 5 arrays even more (size of pitch of holes, ovum groove 3 all can change because of needs).As shown in Figure 3,5 * 5 to be example, so just can once realize a plurality of, cell is fixing in batches, has significantly improved the efficient of injection.Simultaneously, can demarcate through the assist location mark between the array hole, make that this device fixed cell position is clear and definite, the system of being convenient to catches and does not need to search for randomly.
Like Fig. 1 and shown in Figure 2, cell vacuum suck stationary installation also comprises pressure control circuit.Pressure control circuit comprises diaphragm type liquid pump 10, pressure transmitter 12, the electrohydraulic servo valve 11 that is connected with joint 9 and liquid discharge pipe 13.The import of diaphragm type liquid pump 10 is connected with electrohydraulic servo valve 11; The outlet of diaphragm type liquid pump 10 is connected with liquid discharge pipe 13; The input terminus of pressure transmitter 12 is connected between joint 9 and the electrohydraulic servo valve 11, and the output terminal of pressure transmitter 12 is connected to the input terminus of electrohydraulic servo valve 11.Joint 9 is connected to electrohydraulic servo valve 11 by silicone tube, and the other end of electrohydraulic servo valve 11 is connected to the import of diaphragm type liquid pump 10 by silicone tube.Fix or discharges cell, repeatable height through diaphragm type liquid pump 10 work; And device can carry out the fixing of batch cell, high efficiency; And the output terminal of pressure transmitter is received the input terminus of electrohydraulic servo valve, forms closed loop, accurately controls the pressure of liquid in the pipeline.
Comprehensive egative film 1 be provided with depression in order to first rectangular tank that ovum functional sheet 2 is installed and be positioned at first rectangular tank and further second rectangular tank of depression; Said second rectangular tank is a vacuum chamber 8, is provided with the passage that makes liquid communication between said vacuum chamber 8 and the joint 9.
As shown in Figure 4, cell vacuum suck stationary installation also comprises the raceway groove 6 that is formed at the second sperm groove 7 on the comprehensive egative film 1 and connects the first sperm groove 5 and the second sperm groove 7.The width range of raceway groove 6 is 80 ~ 120 μ m., two sperm grooves are interconnected, and splash into the solution that contains sperm to the first sperm groove 5; Because sperm has flowability, the sperm of swimming across the raceway groove 6 entering second sperm groove 7 must be that vitality is stronger, and the sperm of catching is wherein injected; So be provided with, can improve survival rate of embryo.
The principle of work of the cell vacuum suck stationary installation of the embodiment of the invention is: the quality of cell (for example ovum) is minimum; When there being a very little power near it; Just can make its motion; Therefore when 10 work of diaphragm type liquid pump, extract liquid in the vacuum chamber 8 out, the generation negative pressure at micropore 4 places, near cell is drawn close finally to micropore 4 by suction to be fixed.
The cell vacuum suck stationary installation of the embodiment of the invention relies on the work of diaphragm type liquid pump to fix or discharges cell, and is repeatable high; And device can carry out the fixing of batch cell, high efficiency; The output terminal of said pressure transmitter is received the input terminus of electrohydraulic servo valve, forms closed loop, accurately controls the pressure of liquid in the pipeline.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned example embodiment, and under the situation that does not deviate from spirit of the present invention or essential characteristic, can realize the present invention with other specific form.Therefore; No matter from which point; All should regard embodiment as exemplary; And be nonrestrictive, scope of the present invention is limited accompanying claims rather than above-mentioned explanation, therefore is intended to the implication of the equivalents that drops on claim and all changes in the scope are included in the present invention.Should any Reference numeral in the claim be regarded as limit related claim.
In addition; Describing according to embodiment though should be appreciated that this specification sheets, is not that each embodiment only comprises an independently technical scheme; This narrating mode of specification sheets only is for clarity sake; Those skilled in the art should make specification sheets as a whole, and the technical scheme among each embodiment also can form other embodiments that it will be appreciated by those skilled in the art that through appropriate combination.
Claims (7)
1. cell vacuum suck stationary installation; It is characterized in that: it comprises comprehensive egative film, be installed on ovum functional sheet on the comprehensive egative film, be formed at the first sperm groove on the comprehensive egative film; Said ovum functional sheet is provided with the ovum groove of spill; The side of said comprehensive egative film is provided with a joint; Also be provided with the some micropores that are formed at the ovum trench bottom on the said ovum functional sheet, said cell vacuum suck stationary installation also comprises pressure control circuit, and said pressure control circuit comprises diaphragm type liquid pump, pressure transmitter, the electrohydraulic servo valve that is connected with joint and liquid discharge pipe; The import of said diaphragm type liquid pump is connected with electrohydraulic servo valve; The outlet of said diaphragm type liquid pump is connected with liquid discharge pipe, and the input terminus of said pressure transmitter is connected between joint and the electrohydraulic servo valve, and the output terminal of said pressure transmitter is connected to the input terminus of electrohydraulic servo valve.
2. according to the said cell vacuum suck of claim 1 stationary installation; It is characterized in that: said comprehensive egative film be provided with depression in order to first rectangular tank that the ovum functional sheet is installed and be positioned at first rectangular tank and further second rectangular tank of depression; Said second rectangular tank is a vacuum chamber, is provided with the passage that makes liquid communication between said vacuum chamber and the joint.
3. according to the said cell vacuum suck of claim 1 stationary installation, it is characterized in that: also comprise the raceway groove that is formed at the second sperm groove on the comprehensive egative film and connects the first sperm groove and the second sperm groove.
4. according to the said cell vacuum suck of claim 3 stationary installation, it is characterized in that: the width range of said raceway groove is 80 ~ 120 μ m.
5. according to the said cell vacuum suck of claim 1 stationary installation, it is characterized in that: the diameter range of said micropore is 10 ~ 500 μ m.
6. according to the said cell vacuum suck of claim 1 stationary installation, it is characterized in that: said joint is connected to electrohydraulic servo valve by silicone tube, and the other end of said electrohydraulic servo valve is connected to the import of diaphragm type liquid pump by silicone tube.
7. according to the said cell vacuum suck of claim 1 stationary installation, it is characterized in that: said ovum groove is square.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210250586.8A CN102719355B (en) | 2012-07-19 | 2012-07-19 | Cell vacuum absorption fixing device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210250586.8A CN102719355B (en) | 2012-07-19 | 2012-07-19 | Cell vacuum absorption fixing device |
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| CN102719355A true CN102719355A (en) | 2012-10-10 |
| CN102719355B CN102719355B (en) | 2014-07-09 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099237A (en) * | 2014-06-30 | 2014-10-15 | 浙江星博生物科技有限公司 | Sperm optimal selection device based on microtubule passages and optimal selection method |
| CN108660072A (en) * | 2018-05-18 | 2018-10-16 | 苏州大学 | Egg mother cell micromanipulation system and method based on target area Auxiliary support |
| CN115431076A (en) * | 2022-10-08 | 2022-12-06 | 广东长盈精密技术有限公司 | Thin-wall workpiece clamp |
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| CN115431076B (en) * | 2022-10-08 | 2023-08-18 | 广东长盈精密技术有限公司 | Thin-wall workpiece clamp |
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| CN102719355B (en) | 2014-07-09 |
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