CN112998660A - Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument - Google Patents
Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument Download PDFInfo
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- CN112998660A CN112998660A CN202110224882.XA CN202110224882A CN112998660A CN 112998660 A CN112998660 A CN 112998660A CN 202110224882 A CN202110224882 A CN 202110224882A CN 112998660 A CN112998660 A CN 112998660A
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- 238000000799 fluorescence microscopy Methods 0.000 title claims description 14
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- 238000003384 imaging method Methods 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 3
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- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a biological safety bin for a small animal living body fluorescence imager, which comprises a safety bin body and a safety bin cover, wherein the safety bin cover comprises: a cartridge; a bin cover; the bin box is detachably connected with the bin cover so as to be clamped together to form a bin chamber; a high efficiency filter for filtering gas exiting said chamber; a negative pressure system to create a negative pressure environment within the chamber; the carrier plate is arranged in the chamber and used for fixing the living bodies of the small animals; the bin cover is provided with a transparent window for matching with a fluorescence imager to image the living bodies of the small animals on the carrier plate. The invention also provides a fluorescence imager. The carrier plate is adopted to fix the living bodies of the small animals, the structure of the carrier plate is small, the occupied space of a chamber is small, and the miniaturization design of the safety chamber body is facilitated; the carrier plate is simple and convenient to operate for fixing the living bodies of the small animals, and is easy for a laboratory worker to operate with thick gloves; the safety bin body only has the outside trachea of storehouse box and needs the manual switch-on of laboratory technician, and the operation is few, reduces when connecing the trachea the probability that the improper operation leads to toxic gas to reveal.
Description
Technical Field
The invention relates to the technical field of biological safety protection, in particular to a biological safety cabin and a fluorescence imager for a small animal living body fluorescence imager.
Background
In the research work on pathogenic viruses, the living bodies of animals with pathogenic bacteria are usually tested in conjunction with fluorescence imaging analysis. In this experiment, a biosafety chamber is generally used to safely transfer live animals with pathogens. Present biological safety storehouse, including two interior outer storehouses, interior storehouses are used for placing the animal live body, interior storehouse and other indispensable parts are used for holding in the outer storehouse, the structure of two interior outer storehouses leads to the increase of biological safety storehouse whole size, in addition because the operation of packing into biological safety storehouse with the animal live body is the operation in biological safety cabinet, the laboratory technician takes thick rubber gloves to operate, the trachea of two interior outer storehouses is connected and is needed the laboratory technician to put through by hand, complex operation, and easily lead to biological safety storehouse gas leakage because of the inconvenient operation.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the biological safety bin for the small animal living body fluorescence imaging instrument, which has the advantages of small structure, easiness in operation and high safety.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
A first object of the present invention is to provide a biosafety chamber for a small animal in-vivo fluorescence imager, comprising a safety chamber body comprising:
a cartridge;
a bin cover; the bin box is detachably connected with the bin cover so as to be clamped together to form a bin chamber;
a high efficiency filter for filtering gas exiting said chamber;
a negative pressure system to create a negative pressure environment within the chamber;
the carrier plate is arranged in the chamber and used for fixing the living bodies of the small animals;
the bin cover is provided with a transparent window for matching with a fluorescence imager to image the living bodies of the small animals on the carrier plate.
Preferably, a plurality of grooves are formed in the bottom wall of the bin box; the bottom of the carrier plate is provided with a plurality of support columns; and the support column is arranged in the groove to limit the transverse movement of the carrier plate.
Preferably, the carrier plate is provided with four groups of fixing structures; the fixing structure comprises a through groove, a clamp and a screw and nut assembly; the through groove is arranged on the carrier plate; the clamp is connected to the through groove in a sliding mode through the screw nut assembly; four limbs of a living small animal are fixed through the four clamps.
Preferably, the upper surface of the bin box is provided with a circle of sealing groove for clamping the first sealing ring; the first sealing ring is used for sealing the assembly position of the bin box and the bin cover.
Preferably, the high-efficiency filter is fixed on the inner wall of the cartridge and covers the air outlet of the cartridge.
Preferably, the negative pressure system comprises a suction pump arranged outside the cartridge; the air pump is communicated with the air outlet of the bin box.
Preferably, the electronic module in the safety cabin body comprises an air pressure sensor for detecting the air pressure in the cabin.
Preferably, the electronic module further comprises a display for displaying the air pressure value detected by the air pressure sensor;
and/or the electronic module further comprises an alarm for giving out an alarm when the air pressure value detected by the air pressure sensor is out of a pressure range of-500 Pa to-200 Pa.
Preferably, the inner wall of the bin box is provided with a microswitch; when the bin cover is opened and closed, the bin cover is far away from or touches the micro switch so as to control the on-off of the micro switch; and the start and stop of the electronic module are controlled by the on-off of the microswitch.
A second object of the invention is to provide a fluorescence imager comprising an imaging device, further comprising a biosafety chamber as described above for a small animal live fluorescence imager; and the detection port of the imaging equipment is aligned with the transparent window of the safety bin body.
Compared with the prior art, the invention has the beneficial effects that:
the biological safety bin for the small animal living body fluorescence imaging instrument, provided by the invention, adopts the support plate to fix the small animal living body, and the support plate has a small structure, occupies small space of a bin and is beneficial to the miniaturization design of a safety bin body; the carrier plate is simple and convenient to operate for fixing the living bodies of the small animals, and is easy for a laboratory worker to operate with thick gloves; the safety bin body only has the outside trachea of storehouse box and needs the manual switch-on of laboratory technician, and the operation is few, reduces when connecing the trachea the probability that the improper operation leads to toxic gas to reveal.
In a preferred scheme, the safety bin body comprises an air pressure sensor and a display, and the current air pressure in the bin is displayed to a user in real time. Furthermore, the safety cabin body also comprises an alarm, and the alarm gives out an alarm when the air pressure in the current cabin does not meet the limit standard so as to remind a user in time.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented according to the content of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
fig. 1 is a first schematic diagram of the explosion structure of the safe cabin body of the invention;
fig. 2 is a top view of a carrier plate according to the present invention;
FIG. 3 is a cross-sectional view of the security bin body of the present invention;
FIG. 4 is a cross-sectional view of the assembly structure of the base and the fixing frame of the present invention;
FIG. 5 is a perspective view of the assembly structure of the base and the fixing frame of the present invention;
fig. 6 is a schematic diagram of an explosion structure of the safe cabin body of the invention;
fig. 7 is a schematic perspective view of the safety bin body according to the present invention.
In the figure: 1. a secure bin body; 11. a cartridge; 111. a groove; 112. an air outlet; 113. a sealing groove; 114. an air inlet; 115. a first side wall; 1161. a base; 11611. a first recess; 11612. a second recess; 11613. clamping the bulges; 1162. a fixed mount; 11621. a second snap structure; 117. a support; 12. a bin cover; 121. a transparent window; 122. a frame; 1221. a boss; 13. a bin; 20. a high efficiency filter; 30. a carrier plate; 31. a support pillar; 32. a through groove; 33. a clip; 34. a screw; 40. a display; 50. a microswitch; 60. a control panel; 70. a hinge; 80. a locking structure; 81. a first lock catch; 811. buckling grooves; 82. a second lock catch; 821. a rotating part; 822. a buckling part.
Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, which will enable those skilled in the art to practice the present invention with reference to the accompanying specification. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
Example 1
The invention provides a biological safety bin for a small animal living body fluorescence imager, which comprises a safety bin body 1, wherein as shown in figures 1, 3, 6 and 7, the safety bin body 1 comprises:
a magazine 11;
a bin cover 12; the bin box 11 is detachably connected with the bin cover 12 to jointly clamp and form a bin chamber 13; through the detachable connection of the bin box 11 and the bin cover 12, the bin chamber 13 is opened or closed, so that the living bodies of small animals (such as mice) can be conveniently taken and placed;
a high efficiency filter 20 for filtering the gas discharged from the chamber 13; because the living bodies of the small animals have pathogenic bacteria, and in the experimental process, hairs of the living bodies of the small animals are mixed in the air in the bin 13, and after the air in the bin 13 is filtered by the high-efficiency filter 20, the contents of particle impurities and hairs in the air discharged by the safety bin body 1 are reduced, and the safety bin body 1 is ensured to discharge harmful gas to infect experimental environment and experimenters;
a negative pressure system for forming a negative pressure environment in the chamber 13 to prevent leakage of pathogenic bacteria in the chamber 13;
a carrier plate 30 disposed in the chamber 13 for fixing a living body of a small animal; the carrier plate 10 has small volume, occupies small space of the chamber 13, and is beneficial to the miniaturization design of the safety bin body 1; in addition, the living bodies of the small animals with the pathogenic bacteria are directly fixed on the carrier plate 30, and then the carrier plate 30 is placed in the bin 13, so that the operation is convenient and fast, and the laboratory operators can conveniently operate the small animals with thick laboratory operating gloves; compared with the safety bin structure with two bin structures, the operation of connecting the inner bin with the air pipeline is reduced, one-time air connecting pipe operation is reduced, the operation burden of a laboratory worker is reduced, the operation time is shortened, and the probability of toxic gas leakage when the safety bin body 1 is operated is reduced to a certain extent;
the cover 12 is provided with a transparent window 121 for cooperating with a fluorescence imager to image the living small animal on the carrier plate 30. Specifically, the bin 11 is provided with an air outlet 112 and an air inlet 114; the air outlet 112 and the air inlet 114 are respectively provided with an air outlet valve and an air inlet valve (not shown in the figure), and the air outlet valve and the air inlet valve are positioned outside the bin box 11. The safety bin body 1 is operated in a biological safety cabinet of a PS laboratory, the bin cover 12 is opened, the carrier plate 30 is taken out, the small animal living body is fixed by the carrier plate 30, the carrier plate 30 is placed in the bin 13, and the bin cover 12 is covered. An external anesthetic gas pipeline is connected to the gas inlet valve, a gas outlet pipeline is connected to the gas outlet valve, the negative pressure system, the gas outlet valve and the gas inlet valve are opened, and anesthetic gas is introduced into the chamber 13 through the gas inlet 114 on the bin box 11 through the anesthetic gas pipeline so as to anesthetize the living bodies of the small animals and prepare for subsequent fluorescence imaging.
In one embodiment, lid 12 is a flat plate structure. The bin cover 12 comprises a frame 122 and a transparent window 121, wherein a boss 1221 is arranged on the inner wall of the frame 122, and the lower surface of the transparent window 121 abuts against the boss 1221. To facilitate the installation of the transparent window 121, the transparent window 121 is adhered to the boss 1221. The transparent window 121 is an acrylic plate, and has a simple structure. Further, an antireflection film of 400nm to 800nm is plated on the surface of the acrylic plate to improve the light transmittance of the transparent window 121.
In one embodiment, the carrier 30 is a plastic or plastic plate or a steel plate, and has a smooth surface and is easy to clean. In one embodiment, the carrier 30 is rectangular, and has a simple and compact structure.
In one embodiment, as shown in fig. 3 and 5, the bottom wall of the interior of the cartridge 11 is provided with a plurality of grooves 111; the bottom of the carrier plate 30 is provided with a plurality of support columns 31; a support post 31 is disposed in a groove 111 to limit the lateral movement of the carrier 30. Further, in an embodiment, the carrier 30 is rectangular, the number of the grooves 111 is four, and the number of the supporting pillars 31 is four; the four support columns 31 are respectively distributed at four corners of the bottom of the support plate 30 to stably support the support plate 30. After the carrier plate 30 fixes the living small animal, the carrier plate 30 is placed in the chamber 13, the four support columns 30 at the bottom of the support plate 30 are respectively placed in the corresponding grooves 111, and the transverse movement of the support columns 30 is limited by the inner walls of the grooves 111. Further, in order to stabilize the placement of the carrier 30, a plurality of first locking structures are further provided to lock the upper surface of the carrier 30, so as to limit the longitudinal movement of the carrier 30.
In one embodiment, the carrier 30 has four sets of fixing structures; as shown in fig. 2, the fixing structure includes a through slot 32, a clamp 33, and a screw and nut assembly; the through groove 32 is disposed on the carrier plate 30; the clamp 33 is slidably connected to the through slot 32 through the screw nut assembly; four limbs of a living small animal are fixed by the four clamps 33. Specifically, the through groove 32 is inclined toward the central portion of the carrier plate 30 so that the clip moves in a direction approaching or departing from the living body of the small animal. The clamp 33 is located above the carrier plate 30, the screw 34 is inserted into the through hole of the clamp 33 from above the clamp 33 and then passes through the through slot 32, and the nut is matched with the screw from the bottom of the through slot 32 to fasten the clamp 33. When it is desired to adjust the position of the clip 33 in the corresponding through slot 32, the corresponding nut is unscrewed, so that the screw slides in the through slot 32 to adjust the position of the clip 33. Four limbs of the living small animal are clamped by the four clamps 33, so that the body of the living small animal is kept in a stretching state, and subsequent fluorescence imaging analysis is facilitated. Further, in order to prevent the clip 33 from injuring the living body of the small animal, both clipping portions of the clip 33 are covered with a buffer structure such as a buffer tape or a rubber sleeve.
In one embodiment, as shown in fig. 1, the upper surface of the cartridge 11 is provided with a ring of sealing groove 113 for engaging a first sealing ring (not shown); the first sealing ring is used for sealing the assembly position of the bin box 11 and the bin cover 12. Specifically, after the first sealing ring is clamped into the sealing groove 113, the upper surface of the first sealing ring slightly protrudes from the upper surface of the sealing groove 113, and when the bin box 11 is assembled on the bin cover 12, the lower surface of the bin cover 12 presses against the upper surface of the first sealing ring to compress the first sealing ring, so that the sealing effect is achieved, and the negative pressure environment in the bin 13 is maintained.
In one embodiment, as shown in fig. 3, the high efficiency filter 20 is fixed on the inner wall of the cartridge 11 and covers the air outlet 112 of the cartridge 11. When the air in the chamber 13 needs to be exhausted through the air outlet 112 of the chamber 11, the air needs to be filtered through the high efficiency filter 20 to avoid discharging toxic gas.
Further, as shown in fig. 1, 3, and 5, a base 1161 and a fixing frame 1162 are disposed on an inner wall of the first side wall 115 of the bin box 11, where the first side wall is provided with the air outlet 112, the base 1161 is provided with a hollow area, and the base 1161 and the fixing frame 1162 are clamped together to form a cavity for accommodating the high efficiency filter 20; the outer periphery of the high efficiency filter 20 abuts against the inner wall of the base 1161 to limit the movement of the high efficiency filter 20 in the hollow area. The fixing frame 1162 is provided with a plurality of channels for air to pass through so as to ensure the exhaust speed of the safety bin body 1. In an embodiment, as shown in fig. 4, a peripheral side of the inner wall of the base 1161 is recessed to form a first recess 11611, a peripheral side of the high efficiency filter 20 abuts against the inner wall of the first recess 11611, and the side wall of the first recess 11611 and the inner wall of the fixing frame 1162 together form a limiting structure for limiting the movement of the high efficiency filter 20 in the direction away from or close to the first side wall 115. Further, in order to improve the sealing performance of the assembly position of the high efficiency filter 20 and the base 1161, a second recess 11612 is provided at a position of an inner wall of the first recess 11611 away from the first sidewall 115, and an inner surface of the second recess 11612 and an outer circumferential side surface of the high efficiency filter 20 jointly form a space for accommodating a second seal ring. Further, in order to facilitate the assembly of the base 1161 and the fixing frame 1162, a clamping protrusion 11613 is disposed on the outer side of the base 1161, and the fixing frame 1162 is provided with a second fastening structure 11621 matched with the clamping protrusion 11613, so that the base 1161 and the fixing frame 1162 are fastened to achieve rapid assembly. The high efficiency filter 20 is disposed in the cavity formed by the base 1161 and the fixing frame 1162, and the base 1161, the fixing frame 1162, the high efficiency filter 20, and the second sealing ring together form a structure covering the inner contour of the air outlet 112, so that the gas exhausted through the air outlet 112 needs to be filtered by the high efficiency filter 20.
In one embodiment, the high efficiency filter 20 includes a first filter element and a second filter element in sequence along the air flowing direction, the first filter element is used for filtering the particle dust and suspended matter with a particle size of 0.5 μm or more in the air, and the second filter element is used for filtering the toxic particles in the air. Further, the second filter element is activated carbon.
In one embodiment, the negative pressure system comprises a suction pump (not shown) disposed outside the cartridge 11; the air pump is communicated with the air outlet 112 of the bin 11. The air in the chamber 13 is discharged from the air outlet 112 by the operation of the suction pump to reduce the pressure in the chamber 13. The air pump, the first sealing ring and the second sealing ring are arranged, so that the air pressure in the chamber 13 is maintained at-500 Pa to-200 Pa, and pathogenic bacteria in the chamber 13 are prevented from leaking.
In one embodiment, the electronic module in the safety bin body 1 comprises an air pressure sensor for detecting the air pressure in the bin 13. For the convenience of operation and the simplification of the structure of the safety cabin body 1, the electronic module is powered by a battery.
Further, as shown in fig. 1 and 3, the electronic module further includes a display 40 for displaying the air pressure value detected by the air pressure sensor to prompt the laboratory staff of the current pressure condition in the chamber 13;
and/or the electronic module further comprises an alarm for giving out an alarm when the air pressure value detected by the air pressure sensor is out of a pressure range of-500 Pa to-100 Pa.
Specifically, in an embodiment, the electronic module includes an air pressure sensor and a display 40, the air pressure value in the chamber 13 detected by the air pressure sensor is sent to the control board 60 of the safety body 1, and the control board 60 sends the current real-time air pressure to the display, so that the display displays the current air pressure in the chamber 13 in real time. Further, the air pressure sensor is integrated on the control board 60, and the display 40 is disposed below the transparent window 121. The bin box 11 is internally provided with a bracket 117, the bracket 117 is arranged close to the opening of the bin box 12, the display 40 is fixed on the bracket 117, the mounting and dismounting are convenient, and the living condition of the small animal can be checked while the display 40 is checked through the transparent window 121. Further, the electronic module further comprises an alarm, when the air pressure value detected by the air pressure sensor is lower than a defined pressure threshold value (such as-500 Pa to-100 Pa), the control board 60 sends an alarm instruction to the alarm, and the alarm gives an alarm to prompt a laboratory technician to check and adjust the pressure in the chamber 13, so as to warn the user when the laboratory technician does not know the current abnormal condition of the air pressure through the display in time. Further, the alarm is a buzzer. Further, the air pressure sensor and the buzzer are integrated on the control panel 60, so that the occupied space of the bin 13 is reasonably planned, and the miniaturization design of the safety bin body 1 is facilitated.
In yet another embodiment, the electronic module includes a pressure sensor and an alarm, and the user is warned of the abnormal condition of the pressure in the chamber 13 by the alarm.
In one embodiment, as shown in fig. 1 and 6, a micro switch 50 is disposed on an inner wall of the cartridge 11; when the bin cover 12 is opened and closed, the bin cover 12 is far away from or touches the micro switch 50 so as to control the on-off of the micro switch 50; the on-off of the electronic module is controlled by the on-off of the microswitch 50. The principle of the microswitch 50 is that an external force acts on an action reed of the microswitch 50 through a transmission element 51, when the action reed moves to a critical point, instantaneous action is generated, so that a movable contact point at the tail end of the action reed is quickly connected with or disconnected from a fixed contact point, when the acting force on the transmission element 50 is removed, the action reed generates reverse action force, and when the reverse stroke of the transmission element 50 reaches the action critical point of the reed, reverse action is instantaneously completed. Specifically, the top of the transmission element 51 slightly protrudes from the upper surface of the bin 11, and when the bin cover 12 is covered on the bin 11, the lower surface of the bin cover 12 touches the top of the transmission element 51, so that the microswitch 50 is switched on, the power supply module is powered on, and the electronic module (the air pressure sensor, the alarm and the display) is powered on. When the bin cover 12 is opened, the bin cover 12 is far away from the transmission element 51 of the microswitch 50, the external force applied to the transmission element 51 is removed, the microswitch 50 is disconnected, the power supply module stops supplying power, and the electronic module stops working.
In one embodiment, as shown in fig. 3 and 7, one end of the lid 12 is rotatably connected to the box 11 by at least two hinges 70, so as to facilitate the opening and closing of the lid 12. Further, as shown in fig. 6, in order to close the bin cover 12 tightly, the other end of the bin cover 12 is locked on the bin 11 by at least two locking structures 80, so as to prevent the gas in the bin 13 from leaking through the assembly of the bin cover 12 and the bin 11 and polluting the environment. Further, in order to further ensure the confinement property when the bin cover 12 is closed, one side of the bin cover 12 is rotatably connected to the bin 11 through at least two hinges 70, and the other three sides are locked on the bin 11 through the locking structures 80; wherein, one side of the bin cover 12 opposite to the hinge 70 is locked on the bin box 11 through two locking structures 80, and the remaining two sides of the bin cover 12 are respectively locked on the bin box 11 through one locking structure 80. Specifically, in an embodiment, the locking structure 80 includes a first locking catch 81 and a second locking catch 82, and the first locking catch 81 and the second locking catch 82 are respectively disposed on the bin cover 12 and the bin 11. The first lock 81 is disposed in the locking groove 811, and the second lock 82 is disposed in the rotating portion 821 and the locking portion 822. The rotating portion 821 is rotated to change the distance between the fastening portion 822 and the fastening groove 811, so that the fastening portion 822 is fastened in the fastening groove 811 or separated from the fastening groove 811, thereby achieving locking.
Example 2
The invention provides a fluorescence imager, which comprises an imaging device and a biological safety bin, wherein the biological safety bin is used for the small animal living body fluorescence imager; the detection port of the imaging device is aligned with the transparent window 121 of the safety bin body 1. When the fluorescence imaging analysis is performed, the image acquisition port of the imaging device is positioned above the transparent window 121, and the small animal living body on the carrier 30 is aligned through the transparent window 121 to be photographed for the fluorescence imaging analysis.
Compared with the prior art, the biological safety bin for the small animal living body fluorescence imaging instrument is small in structure, the support plate is easy to fix the small animal living body, the probability of toxic gas leakage caused by improper operation of the gas receiving pipe is reduced, the safety bin body is convenient and fast to operate, and the small animal living body is convenient and fast to transport.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; those skilled in the art can readily practice the invention as shown and described in the drawings and detailed description herein; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (10)
1. A biological safety storehouse for little animal live body fluorescence imager, including safe storehouse body (1), its characterized in that, safe storehouse body (1) includes:
a magazine (11);
a bin cover (12); the bin box (11) is detachably connected with the bin cover (12) to jointly clamp and form a bin chamber (13);
a high efficiency filter (20) for filtering gas exiting said chamber (13);
a negative pressure system to create a negative pressure environment within the chamber (13);
a carrier plate (30) arranged in the chamber (13) for fixing the living bodies of the small animals;
the bin cover (12) is provided with a transparent window (121) for matching with a fluorescence imager to image the living small animal on the carrier plate (30).
2. The biosafety chamber for a small animal living body fluorescence imager according to claim 1, characterized in that the bottom wall inside the chamber box (11) is provided with a plurality of grooves (111); the bottom of the carrier plate (30) is provided with a plurality of support columns (31); the supporting column (31) is arranged in the groove (111) to limit the transverse movement of the carrier plate (30).
3. The biosafety chamber for a small animal living body fluorescence imager of claim 1, wherein the carrier plate (30) is provided with four sets of fixing structures; the fixing structure comprises a through groove (32), a clamp (33) and a screw nut assembly; the through groove (32) is arranged on the carrier plate (30); the clamp (33) is connected to the through groove (32) in a sliding mode through the screw nut assembly; four limbs of the living body of the small animal are fixed through the four clamps (33).
4. The biosafety chamber for the fluorescence imaging instrument for the living bodies of the small animals as claimed in claim 1, wherein a circle of sealing groove (113) is formed on the upper surface of the chamber box (11) and used for clamping a first sealing ring; the first sealing ring is used for sealing the assembly position of the bin box (11) and the bin cover (12).
5. The biosafety chamber for a small animal living body fluorescence imager of claim 1, wherein the high efficiency filter (20) is fixed to an inner wall of the chamber box (11) and covers the air outlet (112) of the chamber box (11).
6. The biosafety chamber for a small-animal living-body fluorescence imaging instrument according to any one of claims 1 to 5, characterized in that the negative pressure system comprises a suction pump arranged outside the chamber box (11); the air pump is communicated with an air outlet (112) of the bin box (11).
7. The biosafety chamber for a small animal living body fluorescence imager according to claim 1, wherein the electronic module inside the safety chamber body (1) comprises an air pressure sensor for detecting the air pressure inside the chamber (13).
8. The biosafety chamber for a small-animal living-body fluorescence imager of claim 7, wherein the electronic module further comprises a display (40) to display the air pressure value detected by the air pressure sensor;
and/or the electronic module further comprises an alarm for giving out an alarm when the air pressure value detected by the air pressure sensor is out of a pressure range of-500 Pa to-200 Pa.
9. The biosafety chamber for a small animal living body fluorescence imager according to claim 7 or 8, characterized in that a microswitch (50) is arranged on the inner wall of the chamber box (11); when the bin cover (12) is opened and closed, the bin cover (12) is far away from or touches the micro switch (50) so as to control the on-off of the micro switch (50); the on-off of the electronic module is controlled by the on-off of the microswitch (50).
10. A fluorescence imager comprising an imaging device, characterized by further comprising a biosafety chamber for a small animal in vivo fluorescence imager as described in any of 1-9; the detection port of the imaging device is aligned with the transparent window (121) of the safety cabin body (1).
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