CN112881356B - High-flux fluorescence immunoassay quantitative POCT analysis device - Google Patents
High-flux fluorescence immunoassay quantitative POCT analysis device Download PDFInfo
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0438—Linear motion, sequential
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Abstract
The invention discloses a high-flux fluorescence immunoassay quantitative POCT analysis device, which comprises a shell, wherein a sample seat for placing a plurality of samples, a detection assembly for detecting the samples and a controller for controlling are arranged in the shell; a first electric telescopic rod for driving the detection assembly to move along the horizontal arrangement direction of the sample detection area is arranged in the shell; the sample seats consisting of the first sample seat and the second sample seat are respectively and horizontally arranged in the first shell and the second shell, and the first sample seat and the second sample seat are arranged in a vertically staggered manner; the detection assembly comprises a second electric telescopic rod and a detection head, wherein the second electric telescopic rod is used for driving the detection head to move up and down so as to reach a sample detection area, and the detection head is used for detecting, and the detection head, the first electric telescopic rod and the second electric telescopic rod are respectively electrically connected with the controller. The invention realizes the effects of high flux, portability and cold field use.
Description
Technical Field
The invention relates to the technical field of detection of fluorescence analyzers, in particular to a high-throughput fluorescence immunoassay POCT analysis device.
Background
A fluorescence immunity quantitative POCT analysis device is an instrument for detecting by utilizing fluorescence effect of fluorescent material, wherein, an immunochromatography test strip is provided with fluorescein, after the irradiation of an excitation light source, the fluorescence which is slightly longer than the wavelength of incident light can be emitted after the incident light with a certain wavelength is absorbed, the emitted light disappears once the light source stops irradiation, the fluorescein is taken as a marker and is combined with a known antibody without influencing the immunological characteristic, then the fluorescein marked antibody is taken as a standard reagent and is used for detecting and identifying unknown antigen, the fluorescein which is combined with the antibody is released after the antigen and the antibody carry out immunoreaction, the more the antigen which is immunoreactive with the antibody, the more the released fluorescein is, the more the fluorescence generated by the fluorescence effect is, on the contrary, the generated fluorescence is less, and the fluorescence intensity is converted into an electric signal, the content of the corresponding antigen in the detected object can be known by analyzing the electric signal.
The fluorescence immunoassay POCT analysis device is used for on-site rapid quantitative detection, and is required to have high detection efficiency, small size and convenient carrying and use. In order to increase the detection efficiency, the prior art designs a multi-channel scheme with a rotating disk structure or a linear form, such as a high-throughput automated fluorescence immunoassay analyzer and a control method thereof published as CN110361528A in the prior art, which increases the detection efficiency, but the device is large in size and inconvenient to carry. Especially when using in winter or cold place, ambient temperature is lower, and is great to detecting the accuracy influence, needs to increase temperature control device, preheating device etc. and has increaseed the device size, carries the production degree of difficulty to the miniaturization of device size.
Therefore, it is desirable to design a high-throughput fluorescence immunoassay POCT analysis device capable of solving the above-mentioned technical problems.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
Still another object of the present invention is to provide a high throughput fluorescence immunoassay POCT assay device which can improve the detection efficiency, is portable, and can be used in cold sites.
To achieve these objects and other advantages in accordance with the present invention, there is provided a high throughput immunofluorescence quantitative POCT analysis apparatus, comprising a housing, a sample holder for placing a plurality of samples, a detection assembly for detecting the samples, and a controller for controlling, wherein the housing comprises a first housing and a second housing, the first housing and the second housing are nested in a drawer type, and a stopper is disposed on the first housing for fixing with the second housing; a first opening is formed in one side of the first shell, a second opening is formed in one side of the second shell, and the first shell and the second shell which are sleeved with each other are communicated with each other; a first supporting seat for supporting a first electric telescopic rod is arranged on one side of the interior of the shell along the sleeving sliding direction, and the first electric telescopic rod is used for driving the detection assembly to move along the horizontal arrangement direction of the sample detection area;
the sample holders comprise a first sample holder and a second sample holder, the first sample holder and the second sample holder are respectively horizontally arranged in the first shell and the second shell, the first sample holder and the second sample holder are arranged in a vertically staggered manner, so that the first shell and the second shell cannot collide with each other when sleeved and moved, and a plurality of corresponding sample sockets for inserting a plurality of samples are respectively arranged on the first sample holder and the second sample holder;
the detection assembly comprises a detection head and a second electric telescopic rod, and the second electric telescopic rod is used for driving the detection head to move up and down so as to reach a sample detection area for detection;
the detection head, the first electric telescopic rod and the second electric telescopic rod are respectively electrically connected with the controller.
Preferably, a second supporting seat used for supporting a third telescopic rod is arranged on the other side inside the shell, the third telescopic rod and the first electric telescopic rod are arranged oppositely, the detection assembly is fixed between the telescopic end of the first electric telescopic rod and the telescopic end of the third telescopic rod, and the third telescopic rod is used for assisting the detection assembly to move along the horizontal arrangement direction of the sample detection area. For example, a first supporting seat is disposed on the side opposite to the opening side in the first housing, a second supporting seat is disposed on the side opposite to the opening side in the second housing, and when the first electric telescopic rod extends to drive the detection assembly to move leftward, the third telescopic rod is shortened, so that the detection assembly can stably move along the horizontal arrangement direction of the sample detection area.
Preferably, the limiting part is a buckle or a limiting bolt, and the first shell and the second shell are provided with corresponding buckle holes.
Preferably, the sample holder is provided with a partition plate for placing a plurality of samples at intervals, and a third heating assembly is arranged on the sample holder and below the sample; a heat preservation cover is arranged on the sample seat and above the sample seat for placing the samples, and is used for placing each corresponding sample in a closed space formed by the sample seat and the heat preservation cover; the inner side of the heat-insulating cover is provided with an infrared heating plate, the heat-insulating cover is provided with a corresponding temperature sensor for monitoring the temperature of each sample, the position where the temperature sensor is arranged is not provided with the infrared heating plate, the distance between the temperature sensor and the infrared heating plate is equal to the distance between the corresponding sample and the infrared heating plate, and the temperature of the sample is monitored to be 22-28 ℃; the heat preservation cover above the sample detection area is provided with a detection port for aligning and approaching the detection head of the detection assembly.
Preferably, the method further comprises the following steps: the third shell is used for preheating a sample, an upper end opening and one side opening of the third shell are provided, a cover body capable of opening and closing is arranged on the upper end opening, the third shell is sleeved with the first shell in a drawer type mode through the one side opening, the sleeved position of the third shell is located on the opposite side of the sleeved position of the second shell, and a limiting piece used for being fixed with the third shell is arranged on the first shell; and a first heating assembly and a temperature sensor for monitoring the temperature in the third shell are arranged in the third shell. When the third housing is used, the temperature in the third housing is monitored at 20-25 ℃.
Preferably, a slide way for the first sliding block to slide up and down is arranged on the side wall of the first shell on the side sleeved with the third shell, the first heating component is a heating lath, one end of the first heating component is hinged with the lowest part of the side wall of the third shell far away from the first shell, and the other end of the first heating component is hinged with the first sliding block; when the third shell is pushed into the first shell, the first sliding block drives the other end of the first heating assembly to move to the upper part of the side wall of the first shell, and the first heating assembly is clamped between the side wall of the third shell and the side wall of the first shell; when the third shell is pulled out of the first shell, the first sliding block drives the other end of the first heating assembly to move to the lower portion of the side wall of the first shell, an included angle of 20-40 degrees is formed between the first heating assembly and the bottom wall, a heat insulation coating is arranged on the upper surface of the first heating assembly and is a rough surface, and a heat conduction coating is arranged on the lower surface of the first heating assembly and is a rough surface.
Preferably, a slide way for the second sliding block to slide up and down is arranged on the side wall of a third shell far away from the first shell, and the heating device also comprises a second heating component in the third shell, wherein the second heating component is a heating lath, one end of the second heating component is hinged with the bottom wall of the third shell close to the first shell, and the other end of the second heating component is hinged with the second sliding block; when the third shell is pushed into the first shell, the second sliding block drives the other end of the second heating assembly to move to the upper part of the side wall of the third shell, and the second heating assembly is clamped between the side wall of the third shell and the side wall of the first shell; when the third shell is pulled out of the first shell, the second sliding block drives the other end of the second heating assembly to move to the lower portion of the side wall of the third shell, the second heating assembly and the bottom wall form an included angle of 20-40 degrees, a heat insulation coating is arranged on the upper surface of the second heating assembly and is a rough surface, a heat conduction coating is arranged on the lower surface of the second heating assembly and is a rough surface, and the first assembly and the second assembly are alternately arranged at intervals along the parallel direction of the hinged side.
Preferably, the lid is articulated with the upper end of third casing lateral wall, be provided with magnet on the opening of third casing upper end, be provided with magnet with lid articulated third casing lateral wall upper portion, be provided with corresponding magnet on the upper and lower side of lid for the lid is fixed with the magnetic attraction that third casing upper end opening lid closed and opened.
Preferably, a through hole is provided in the first casing side wall on the side fitted with the third casing, for communicating the first casing with the third casing.
One way of realizing drawer type sleeving of the invention is as follows: the first shell is provided with a first shell side wall, a second shell side wall and a third shell side wall, wherein the first shell is sleeved with the third shell, and the other wall bodies of the first shell are hollow wall bodies. And the first heating assembly, the second heating assembly, the third heating assembly, the infrared heating plate and the temperature sensor are respectively and electrically connected with the controller.
The invention at least comprises the following beneficial effects:
the high-throughput fluorescence immunoassay quantitative POCT analysis device provided by the invention realizes the effects of high throughput and portability. When the size of the device needs to be reduced, the second shell is sleeved in the first shell and is fixed through the limiting piece, so that the device is convenient to carry; use more sample socket at needs to when improving detection efficiency, stretch out the second casing in following first casing, and fixed through the locating part, can be in order to use more sample socket simultaneously, for example first sample seat sets up eight sample socket passageways, and the second sample seat sets up eight sample socket passageways, just can detect by a wheel to sixteen samples, increases the channel volume under the portable device size for detection efficiency.
The high-throughput fluorescence immunoassay quantitative POCT analysis device can solve the problem of accurate heating of a sample reaction area, improves the reaction effect of the sample, and is beneficial to improving the accurate detection effect.
The infrared heating plate is arranged on the inner side of the heat-insulating cover, and heat of the infrared heating plate is directly radiated on the reaction area of the sample, so that the temperature of the sample is well controlled, the temperature of the reaction area of the sample can be accurately controlled, the reaction of the sample on the sample seat is facilitated, the reaction time is shortened, redundant reaction devices outside the device are reduced, the reaction efficiency is improved, and the detection accuracy is improved. The problem that the reaction zone is heated inaccurately through air heat transfer or sample heat transfer when the sample is heated at the bottom conventionally, the reaction efficiency of the reaction zone is influenced, even the temperature required by the reaction cannot be reached, and the detection influence on a cold site is large is solved.
And fourthly, the third shell is used for preheating the sample before the sample is dripped into the object to be tested and preheating the object to be tested, so that the influence of cold environment on the sample and the object to be tested is avoided. According to the invention, the first heating assembly and the second heating assembly are alternately arranged at intervals, and when the third shell is pulled out of the first shell, the first heating assembly and the second heating assembly are obliquely distributed with the bottom surface, so that the temperature rise and the temperature control in the third shell are facilitated, and the samples are erected among the first heating assembly, the second heating assembly and the bottom wall of the third shell at intervals, so that the heat absorption and the temperature control of the samples are facilitated.
And fifthly, the through hole is formed in the side wall of the first shell at the side sleeved with the third shell, so that when the device is used in a cold environment, the interior of the device is conveniently preheated, and the normal operation of the device is facilitated.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic structural diagram of an implementation form of a reduced size of the high-throughput fluorescence immunoassay POCT analysis apparatus of the present invention;
FIG. 2 is a schematic diagram of the high throughput fluorescence immunoassay POCT analyzer according to the present invention;
FIG. 3 is a schematic view of one implementation of the inner portion of FIG. 2 planed along direction A-A;
fig. 4 is a schematic view of another embodiment of the inner portion of fig. 2 along the direction a-a.
Wherein, the first casing 1; a cover body 2; a third housing 3; a first sample holder 4; a sample socket 5; a second sample holder 6; a second housing 7; a display screen 8; a second support base 11; a third telescopic rod 12; a second electric telescopic rod 13; a detection assembly 14; a detection head 15; a first electric telescopic rod 16; a first support base 17; a first heating assembly 21; a second heating assembly 22; a second slider 23; a second hinge 24; a first slider 25; a first hinge 26; a third heating assembly 31; a heat-insulating cover 32; a detection port 33.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It should be noted that in the description of the present invention, the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of describing the present invention and simplifying the description, and does not indicate or imply that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
1-3, the high-throughput fluorescence immunoassay POCT analysis apparatus comprises a housing, in which a sample holder for placing a plurality of samples, a detection component 14 for detecting the samples, and a controller for controlling are disposed, wherein the housing comprises a first housing 1 and a second housing 7, the first housing 1 and the second housing 7 are disposed in a drawer-type manner, and a limiting member for fixing with the second housing 7 is disposed on the first housing 1; a first opening is formed in one side of the first shell 1, a second opening is formed in one side of the second shell 7, and the first shell 1 and the second shell 7 are used for communicating with each other; a first supporting seat 17 for supporting a first electric telescopic rod 16 is arranged on one side of the inside of the shell along the sleeving sliding direction, and the first electric telescopic rod 16 is used for driving the detection assembly 14 to move along the horizontal arrangement direction of the sample detection area;
the sample holder comprises a first sample holder 4 and a second sample holder 6, the first sample holder 4 and the second sample holder 6 are respectively horizontally arranged in the first shell 1 and the second shell 7, the first sample holder 4 and the second sample holder 6 are arranged in a vertically staggered manner, so that the first shell 1 and the second shell 7 cannot collide with each other when being sleeved and moved, and the first sample holder 4 and the second sample holder 6 are respectively provided with a plurality of corresponding sample sockets 5 for inserting a plurality of samples;
the detection assembly 14 comprises a detection head 15 and a second electric telescopic rod 13, and the second electric telescopic rod 13 is used for driving the detection head 15 to move up and down so as to reach a sample detection area for detection;
the detection head 15, the first electric telescopic rod 16 and the second electric telescopic rod 13 are respectively electrically connected with the controller.
The test head 15 is a prior art test head 15 that includes a light source and a silicon photocell. During the detection of the sample, for example: each sample dropwise add determinand and in inserting the sample holder, when the sample reaction of label one or record sample serial number ended, the control lamp source shines the light, shine the light of lamp source on the detection zone of corresponding sample, on the reactant promptly, then refract the light source to the silicon photocell on through the sample, thereby form the electric current, the information of formation electric current sends the controller, the controller conveys the display screen 8 after with information processing on, show the inspection result, perhaps save and be used for conveying next computer or printer to print on the memory, accomplish the sample detection of label one. When a plurality of samples need to be detected, start first electric telescopic handle 16, will detect head 15 and remove next sample, and label two or record sample serial number, when the reaction is ended, the control lamp source gives off the radiance, with the light irradiation of lamp source to the detection zone that corresponds the sample on, be on the reactant, then refract the light source to silicon photocell through the sample on, thereby form the electric current, the information that forms the electric current sends the controller, the controller conveys behind the information processing on display screen 8, show the inspection result, or save and be used for conveying next computer or printer to print on the memory, accomplish the sample detection of label two, detect a plurality of samples according to this. After the first row of samples are detected, for example, after the samples in the first sample holder 4 are detected, the process can enter the sample detection of the second sample holder 6, specifically, the first electric telescopic rod 16 is started, the detection head 15 is moved to the upper part of the sample detection area of the second sample holder 6, then the second electric telescopic rod 13 is started to move the detection head 15 downwards to reach the sample detection area of the second sample holder 6, and a plurality of samples in the second sample holder 6 are detected in sequence according to the mode of detecting the samples in the first sample holder 4. In the detection process, the detected sample can be taken out and replaced by the sample to be detected, and the controller controls one cycle and the other cycle for detection, so that high-flux detection is realized.
When the size of the device needs to be reduced, the high-throughput fluorescence immunoassay quantitative POCT analysis device is characterized in that a second shell 7 is sleeved into a first shell 1 and is fixed through a limiting piece so as to be convenient to carry; use more sample socket 5 at needs to when improving detection efficiency, stretch out second casing 7 from first casing 1 in, and fixed through the locating part, can be with using more sample socket 5 simultaneously, for example first sample seat 4 sets up eight sample sockets 5, and second sample seat 6 sets up eight sample sockets 5, just can detect sixteen samples by a wheel pair for detection efficiency. The device of the invention realizes the effects of high flux and portability.
On the basis of the above implementation form, a second supporting seat 11 for supporting a third telescopic rod 12 is arranged on the other side inside the shell, the third telescopic rod 12 is arranged opposite to the first electric telescopic rod 16, the detection assembly 14 is fixed between the telescopic end of the first electric telescopic rod 16 and the telescopic end of the third telescopic rod 12, and the third telescopic rod 12 is used for assisting the detection assembly 14 to move along the horizontal arrangement direction of the sample detection areas. For example, a first supporting seat 17 is provided at the side opposite to the opening side in the first housing 1, a second supporting seat 11 is provided at the side opposite to the opening side in the second housing 7, and when the first electric telescopic rod 16 extends to drive the detecting member 14 to move leftward, the third telescopic rod 12 is shortened to stably move the detecting member 14 in the horizontal arrangement direction of the sample detecting section.
On the basis of the above implementation form, the limiting member is a buckle or a limiting bolt, and the first casing 1 and the second casing 7 are provided with corresponding buckle holes.
On the basis of the above implementation form, referring to fig. 4, a partition board is arranged on the sample holder for placing a plurality of samples at intervals, and a third heating assembly 31 is arranged on the sample holder and below the sample; a heat preservation cover 32 is arranged on the sample seat and above the sample seat for placing the samples, and is used for placing each corresponding sample in a closed space formed by the sample seat and the heat preservation cover 32; an infrared heating plate is arranged on the inner side of the heat-insulating cover 32, a corresponding temperature sensor for monitoring the temperature of each sample is arranged on the heat-insulating cover 32, the infrared heating plate is not arranged at the position where the temperature sensor is arranged, the distance between the temperature sensor and the infrared heating plate is equal to the distance between the corresponding sample and the infrared heating plate, and the temperature of the sample is monitored to be 22-28 ℃; the heat-insulating cover 32 above the sample detection area is provided with a detection port 33 for aligning and approaching the detection head 15 of the detection assembly 14.
Through being provided with the infrared heating board in heat preservation cover 32 inboard, the heat direct radiation of infrared heating board plays fine temperature control to the sample on the reaction zone of sample, can more accurate control sample reaction zone's temperature, is favorable to the sample to react on the sample seat, shortens reaction time and reduces the outer unnecessary reaction unit of this device, improves reaction efficiency to and be favorable to improving the detection accuracy. The problem that the reaction zone is heated inaccurately through air heat transfer or sample heat transfer when the sample is heated at the bottom conventionally, the reaction efficiency of the reaction zone is influenced, even the temperature required by the reaction cannot be reached, and the detection influence on a cold site is large is solved. If near-infrared fluorescent molecules are used as marks, near-infrared scanning is adopted during detection, the infrared heating plate of the heat-insulating cover 32 above the corresponding sample can be closed when the corresponding sample is detected, and the infrared heating plate is opened after the detection is finished to heat the sample to be detected for replacement.
On the basis of the above implementation form, the method further includes: the third shell 3 is used for preheating a sample, the upper end opening and one side opening of the third shell 3 are provided, the upper end opening is provided with a cover body 2 which can be opened and closed, the third shell 3 is sleeved with the first shell 1 in a drawer type through the one side opening, the sleeved position of the third shell 3 is positioned on the opposite side of the sleeved position of the second shell 7, and the first shell 1 is provided with a limiting piece which is used for being fixed with the third shell 3; a first heating assembly 21 and a temperature sensor for monitoring the temperature in the third housing 3 are arranged in the third housing 3. When the third housing 3 is used, the temperature inside the third housing 3 is monitored at 20-25 ℃.
On the basis of the above implementation form, a slide way for the first sliding block 25 to slide up and down is provided on the side wall of the first casing 1 at the side sleeved with the third casing 3, the first heating component 21 is a heating lath, one end of the first heating component is hinged with the lowest part (the first hinge part 26) of the side wall of the third casing 3 far away from the first casing 1, and the other end is hinged with the first sliding block 25; when the third casing 3 is pushed into the first casing 1, the first slider 25 drives the other end of the first heating element 21 to move to the upper part of the side wall of the first casing 1, and the first heating element 21 is clamped between the side wall of the third casing 3 and the side wall of the first casing 1; when the third casing 3 is pulled out from the first casing 1, the first slider 25 drives the other end of the first heating assembly 21 to move to the lower part of the side wall of the first casing 1, and the first heating assembly 21 and the bottom wall form an included angle of 20-40 degrees, the upper surface of the first heating assembly 21 is provided with a heat insulation coating and is a rough surface, and the lower surface of the first heating assembly 21 is provided with a heat conduction coating and is a rough surface.
On the basis of the above implementation form, a slide way for the second sliding block 23 to slide up and down is provided on the side wall of the third casing 3 far away from the first casing 1, and in the third casing 3, the second heating assembly 22 is further included, the second heating assembly 22 is a heating plate strip, one end of the second heating assembly 22 is hinged with the bottom wall (second hinge part 24) of the third casing 3 near the first casing 1, and the other end is hinged with the second sliding block 23; when the third casing 3 is pushed into the first casing 1, the second slider 23 drives the other end of the second heating assembly 22 to move to the upper part of the side wall of the third casing 3, and the second heating assembly 22 is clamped between the side wall of the third casing 3 and the side wall of the first casing 1; when the third casing 3 is pulled out from the first casing 1, the second slider 23 drives the other end of the second heating assembly 22 to move to the lower part of the side wall of the third casing 3, the second heating assembly 22 and the bottom wall form an included angle of 20-40 degrees, the upper surface of the second heating assembly 22 is provided with a heat insulation coating and is a rough surface, the lower surface of the second heating assembly 22 is provided with a heat conduction coating and is a rough surface, and the first heating assembly 21 and the second heating assembly 22 are alternately arranged along the parallel direction of the hinged side at intervals. In the position of the drawing, the surface on which the sample insertion port 5 is provided is the front surface, and the first heating unit 21 and the second heating unit 22 are alternately arranged at intervals in the front-rear direction.
The third shell 3 is used for preheating the sample before the sample is dripped into the object to be tested and preheating the object to be tested, so that the influence of cold environment on the dripping of the sample and the object to be tested is avoided. According to the invention, the first heating assemblies 21 and the second heating assemblies 22 are alternately arranged at intervals, and when the third shell 3 is pulled out of the first shell 1, the first heating assemblies 21 and the second heating assemblies 22 are obliquely distributed with the bottom surface, so that the temperature rise and the temperature control in the third shell 3 are facilitated, and the samples are erected among the first heating assemblies 21, the second heating assemblies 22 and the bottom wall of the third shell 3 at intervals, so that the heat absorption and the temperature control of the samples are facilitated.
On the basis of the above-mentioned realization form, lid 2 is articulated with the upper end of 3 lateral walls of third casing, be provided with magnet on the opening of 3 upper ends of third casing, be provided with magnet with 3 lateral wall upper portions of the articulated third casing of lid 2, be provided with corresponding magnet on the upper and lower side of lid 2 for lid 2 and 3 upper end opening of third casing lid close with the magnetic attraction of opening fixed.
On the basis of the above implementation form, a through hole is provided on the side wall of the first casing 1 on the side of being nested with the third casing 3, and is used for communicating the first casing 1 with the third casing 3.
According to the invention, the through hole is formed in the side wall of the first shell 1 at the side sleeved with the third shell 3, so that when the device is used in a cold environment, the interior of the device can be preheated conveniently, and the normal operation of the device is facilitated.
One way of realizing drawer type sleeving of the invention is as follows: the first casing 1 is a hollow wall except the side wall of the first casing 1 at the side sleeved with the third casing 3, and the other walls of the first casing 1 are hollow walls for the second casing 7 and the third casing 3 to be sleeved with the first casing 1 in a drawer type through the hollow walls. And the first heating unit 21, the second heating unit 22, the third heating unit 31, the infrared heating panel and the temperature sensor are electrically connected to the controller, respectively.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (9)
1. The high-throughput fluorescence immunoassay quantitative POCT analysis device comprises a shell, wherein a sample seat for placing a plurality of samples, a detection component for detecting the samples and a controller for controlling are arranged in the shell,
the shell comprises a first shell and a second shell, the first shell and the second shell are sleeved in a drawer type, and a limiting piece used for being fixed with the second shell is arranged on the first shell; a first opening is formed in one side of the first shell, a second opening is formed in one side of the second shell, and the first shell and the second shell are used for communicating the first shell and the second shell; a first supporting seat for supporting a first electric telescopic rod is arranged on one side of the interior of the shell along the sleeving sliding direction, and the first electric telescopic rod is used for driving the detection assembly to move along the horizontal arrangement direction of the sample detection area;
the sample holder comprises a first sample holder and a second sample holder, the first sample holder and the second sample holder are respectively horizontally arranged in the first shell and the second shell, the first sample holder and the second sample holder are arranged in a vertically staggered manner, and a plurality of corresponding sample sockets for inserting a plurality of samples are respectively arranged on the first sample holder and the second sample holder;
the detection assembly comprises a detection head and a second electric telescopic rod, and the second electric telescopic rod is used for driving the detection head to move up and down so as to reach a sample detection area for detection;
the detection head, the first electric telescopic rod and the second electric telescopic rod are respectively electrically connected with the controller.
2. The high throughput immunofluorescence quantitative POCT analysis apparatus according to claim 1, wherein a second support base for supporting a third telescopic rod is provided on the other side inside the housing, the third telescopic rod is disposed opposite to the first telescopic rod, the detection module is fixed between the telescopic end of the first telescopic rod and the telescopic end of the third telescopic rod, and the third telescopic rod is used to assist the detection module to move along the horizontal arrangement direction of the sample detection zones.
3. The high-throughput immunofluorescence quantitative POCT analysis apparatus according to claim 1, wherein the position-limiting member is a buckle or a position-limiting bolt, and the first housing and the second housing are provided with corresponding buckle holes.
4. The high throughput immunofluorescent quantitative POCT analysis apparatus according to claim 1, wherein a partition is provided on the sample holder for placing a plurality of samples at intervals, and a third heating unit is provided on the sample holder below the sample placement; a heat preservation cover is arranged on the sample seat and above the sample seat for placing the samples, and is used for placing each corresponding sample in a closed space formed by the sample seat and the heat preservation cover; the inner side of the heat-insulating cover is provided with an infrared heating plate, the heat-insulating cover is provided with a corresponding temperature sensor for monitoring the temperature of each sample, the position where the temperature sensor is arranged is not provided with the infrared heating plate, the distance between the temperature sensor and the infrared heating plate is equal to the distance between the corresponding sample and the infrared heating plate, and the temperature of the sample is monitored to be 22-28 ℃; the heat preservation cover above the sample detection area is provided with a detection port for aligning and approaching the detection head of the detection assembly.
5. The high throughput fluoroimmunoassay POCT assay device of any of claims 1-4, further comprising: the third shell is used for preheating a sample, an upper end opening and one side opening of the third shell are provided, a cover body capable of opening and closing is arranged on the upper end opening, the third shell is sleeved with the first shell in a drawer type mode through the one side opening, the sleeved position of the third shell is located on the opposite side of the sleeved position of the second shell, and a limiting piece used for being fixed with the third shell is arranged on the first shell; and a first heating assembly and a temperature sensor for monitoring the temperature in the third shell are arranged in the third shell.
6. The high-throughput immunofluorescence quantitative POCT analysis apparatus according to claim 5, wherein a slide for the first slider to slide up and down is provided on the side wall of the first housing which is nested in the third housing, the first heating element is a heating plate strip, one end of which is hinged to the lowest part of the side wall of the third housing which is far away from the first housing, and the other end is hinged to the first slider; when the third shell is pushed into the first shell, the first sliding block drives the other end of the first heating assembly to move to the upper part of the side wall of the first shell, and the first heating assembly is clamped between the side wall of the third shell and the side wall of the first shell; when the third shell is pulled out of the first shell, the first sliding block drives the other end of the first heating assembly to move to the lower portion of the side wall of the first shell, an included angle of 20-40 degrees is formed between the first heating assembly and the bottom wall, a heat insulation coating is arranged on the upper surface of the first heating assembly and is a rough surface, and a heat conduction coating is arranged on the lower surface of the first heating assembly and is a rough surface.
7. The high-throughput immunofluorescence quantitative POCT analysis apparatus according to claim 6, wherein a slide for the second slider to slide up and down is provided on a side wall of a third housing away from the first housing, the third housing further comprises a second heating element, the second heating element is a heating plate strip, one end of the second heating element is hinged to a bottom wall of the third housing close to the first housing, and the other end is hinged to the second slider; when the third shell is pushed into the first shell, the second sliding block drives the other end of the second heating assembly to move to the upper part of the side wall of the third shell, and the second heating assembly is clamped between the side wall of the third shell and the side wall of the first shell; when the third shell is pulled out of the first shell, the second sliding block drives the other end of the second heating assembly to move to the lower portion of the side wall of the third shell, the second heating assembly and the bottom wall form an included angle of 20-40 degrees, a heat insulation coating is arranged on the upper surface of the second heating assembly and is a rough surface, a heat conduction coating is arranged on the lower surface of the second heating assembly and is a rough surface, and the first heating assembly and the second heating assembly are alternately arranged at intervals along the parallel direction of the hinged side.
8. The high-throughput quantitative fluorescence immunoassay POCT analysis device as claimed in claim 7, wherein the cover is hinged to the upper end of the side wall of the third casing, a magnet is provided on the upper opening of the third casing, a magnet is provided on the upper portion of the outer side wall of the third casing hinged to the cover, and corresponding magnets are provided on the upper and lower sides of the cover for the magnetic attraction fixation of the cover and the upper opening of the third casing.
9. The high throughput immunofluorescent quantitative POCT analysis apparatus according to claim 8, wherein a through hole is provided on a side wall of the first casing on the side nested with the third casing, for communicating the first casing with the third casing.
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