CN112852671A - Microbial preparation for promoting corn growth - Google Patents
Microbial preparation for promoting corn growth Download PDFInfo
- Publication number
- CN112852671A CN112852671A CN202110152711.0A CN202110152711A CN112852671A CN 112852671 A CN112852671 A CN 112852671A CN 202110152711 A CN202110152711 A CN 202110152711A CN 112852671 A CN112852671 A CN 112852671A
- Authority
- CN
- China
- Prior art keywords
- corn
- azospirillum
- bacteria
- hydrogenotrophic
- cgmcc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a compound microbial agent, which is prepared by mixing fermentation liquor of azotobacter and hydrogen-oxidizing bacteria, wherein the azotobacter is Azospirillum melinii (CGMCC 1.5340) and hydrogenotrophic yellow bacteria (hydrogenotropga flava) CGMCC 1.8793. The invention utilizes the correlation action relationship between the azospirillum melitensis and the hydrogenotrophic bacteria, and obtains the compound microbial inoculum capable of promoting the corn by adjusting the volume ratio of the fermentation liquor between the azospirillum melitensis and the hydrogenotrophic bacteria. The preparation can be prepared by simply mixing two strains, is simple to prepare, has the effects of promoting the growth of the stem root and thickening the leaf of the corn, has the beneficial effects of accelerating the rooting of the seed and shortening the germination time of the seed, and also has a certain insect-resistant effect on preventing and treating the corn borer.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a compound microbial preparation for promoting corn growth.
Background
Corn is one of the main grain crops in China, and the cultivation and management of the corn at present mainly comprises the main production links of corn variety selection, seed treatment, land preparation, sowing, field management and the like. The existing fertilizer application technology and the crop root strengthening and raising technology can ensure that corn plants grow vigorously; or the corn seeds are coated to meet the requirements of healthy germination for insect prevention, bacteria prevention and corrosion prevention, and the fertilizer is applied to prevent the plant from being weak and lodging and increase the width of the corn leaf surface, so that the lodging of the plant is reduced and the photosynthesis of the plant is increased.
The existing mainstream planting mode growers neglect crop root system maintenance and excessively depend on using agricultural fertilizers, so that the root system effect of crops cannot be better played in field management before sowing, and the fertilizer is used to overflow in the crop growth period after sowing to exceed the crop requirement level. If the fertilizer is applied to corn farmlands for a long time, nutrients of the fertilizer cannot be effectively absorbed and utilized by crops, and chemical substances such as nitrogen, phosphorus, potassium and the like are easily solidified by soil to form various chemical salts which are accumulated in the soil to cause structural imbalance of soil nutrients, deterioration of physical properties and standard exceeding of harmful metals and harmful germs in partial plots, so that the soil properties are deteriorated. The long-term application of the fertilizer can cause the loss of elements such as calcium, magnesium and the like, so that the soil is continuously acidified, the safety and the usability of the land are threatened, the land recycling area is reduced, and the productivity is finally lost. In addition, the lodging problem of corn is related to the long-term use of nitrogen fertilizer, once the crop lodging grain quality is reduced and the yield is reduced sharply, the Chinese grain safety is threatened. Moreover, the application amount of the fertilizer is difficult to control, and the corn seedling burning phenomenon is easily caused once too much fertilizer is applied.
The fertilizer has the effect of increasing the yield of crops, but the application of the fertilizer is harmful to soil. Calcium phosphate contains a large amount of free acid and, when applied in large quantities in succession, can cause acidification of the soil. The calcium magnesium phosphate fertilizer contains 25 to 30 percent of lime, and the soil alkalinity and the physicochemical property are worsened by large-scale application. Excessive application of calcium phosphate can cause zinc in soil to react with excessive phosphorus, so that zinc phosphate precipitate is generated and cannot be absorbed by crops. If the fertilizer is excessively applied to the soil, the consumed carbon comes from soil organic matters, the content of the organic matters is low, the activity of microorganisms is influenced, and therefore the formation of a soil aggregate structure is influenced, and soil hardening is caused. The content of organic matters is an important index of soil fertility and a granular structure, and the reduction of the organic matters causes soil hardening.
Although the application of the chemical fertilizer can promote the growth of corn plants to a certain extent, the continuous long-term use of the chemical fertilizer can lead to excessive growth of crop plants, soft and weak stems, thick and green leaves, easy lodging and pest and disease infection, green and late maturity, difficult drying of seeds and easy mildew; the nitrate content of the vegetables is increased, the rotten vegetable phenomenon is prominent, the flavor and the color of some melons and fruits are deteriorated, and adverse effects on human health can be caused.
The corn coated seeds can achieve the effect of maintaining the health of the seedling stage transiently, but kill beneficial bacteria and harmful bacteria on the surfaces of the seeds, and can also cause irreversible damage to beneficial microbial colonies in the soil after sowing, thereby destroying the microbial ecological balance.
Influenced by the current planting mode, long-term fertilizer application, durable high-density continuous cropping of crops and lack of soil maintenance cause the microbial flora of the soil to be adversely affected for a long time. Death of beneficial bacteria in soil and mass propagation of pathogenic bacteria cause greatly increased risk of crop diseases and insect pests. The root system of the crops is lack of functional beneficial microorganisms, and the inhibition and antagonism on the proliferation of harmful bacteria are lacked, so that the disease degree and frequency of the crops are increased. Beneficial bacteria are lacked in most cultivated soil in China, particularly in greenhouse soil, plant roots are shallow, and moisture and nutrition cannot be well absorbed. Chemical fertilizer abuse harms agricultural soil and needs to reduce the use of chemical fertilizers. According to the method, the yield of the corn field is not improved, and the problem of applying chemical fertilizers to the corn cannot be solved under the condition of low cost.
Disclosure of Invention
In order to solve the technical problems, the invention provides a compound microbial agent capable of promoting the growth of corn. The microbial agent can improve the growth performance of the corn through a composite microbial agent compounded by azotobacter and hydroxide bacteria. The Azospirillum melinii (Azospirillum melinis) CGMCC1.5340 and the hydrogenotrophic bacterium (Hydrogenophaga flava) CGMCC1.8793 used in the invention are purchased from China general microbiological culture Collection center (CGMCC). Azospirillum melinii (Azospirillum melinis) CGMCC1.5340 and hydrogenotrophus flavus (Hydrogenophaga flava) CGMCC1.8793 have been deposited in CGMCC No. 10/24 and No. 10/21 in 2005 respectively.
The invention realizes the purpose of the invention by the following technical scheme:
in the first aspect, the compound microbial agent is prepared by mixing fermentation liquor of azotobacter and hydrogen-oxidizing bacteria, wherein the azotobacter is Azospirillum melinis (CGMCC 1.5340) and hydrogenotrophus xanthus (Hydrogenophaga flava) CGMCC 1.8793. According to the invention, through a large number of strain screening combinations, the combination of the azospirillum meliloti with the preservation number of CGMCC1.5340 and the fermentation liquid of the hydrogenotrophic yellow bacterium with the preservation number of CGMCC1.8793 can play a role in synergy, and the growth performance of the corn is improved.
Preferably, the volume ratio of the fermentation liquid of the azospirillum melitensis to the fermentation liquid of the hydrogenotrophus flavus is 1: 2-5. More preferably, the volume ratio of the fermentation broth of the azospirillum melitensis to the fermentation broth of the hydrogenotrophus flavus is 1: 3. A large number of experimental researches show that when the volume ratio of the fermentation liquor of azospirillum meliloti to the fermentation liquor of hydrogenotrophus flavus is 1: 2-5, the growth performance of corn can be effectively improved by the compatibility of the azospirillum meliloti and the hydrogenotrophus flavus, and particularly, the effect is most remarkable when the volume ratio is 1: 3.
Preferably, the number of viable bacteria in the mixed fermentation liquid is 1.3 multiplied by 108～2.5×1010One per ml.
The Azospirillum melitensis (CGMCC 1.5340) and the hydrogenotrophic fungus (Hydrogenophaga flava) CGMCC1.8793 are both purchased from China general microbiological culture Collection center (CGMCC).
In a second aspect, the invention provides the application of the microbial agent in a plant growth promoter.
Preferably, the plant is maize.
The invention provides an application of the microbial agent in a corn insect-resistant preparation.
Preferably, the insect-resistant is corn borer. The invention can effectively prevent and control the damage of the corn borers to the corns.
The invention has the beneficial effects that: the invention utilizes the correlation action relationship between the azospirillum melitensis and the hydrogenotrophic bacteria, and obtains the compound microbial inoculum capable of promoting the corn by adjusting the volume ratio of the fermentation liquor between the azospirillum melitensis and the hydrogenotrophic bacteria. The preparation can be prepared by simply mixing two strains, is simple to prepare, has the effects of promoting the growth of the stem root and thickening the leaf of the corn, has the beneficial effects of accelerating the rooting of the seed and shortening the germination time of the seed, and also has a certain insect-resistant effect on preventing and treating the corn borer.
Drawings
FIG. 1 is a comparison of stem cross-sections of a corn control group and an experimental group (A is the control group; B is the experimental group)
FIG. 2 is a comparison of the cross section of the main vein between the control group and the experimental group (A is the control group; B is the experimental group)
FIG. 3 is a comparison of leaf thickness cross-sections of a corn control group and an experimental group (A is the control group; B is the experimental group)
FIG. 4 is a cross-sectional view of the roots of the corn control group and the experimental group (A is the control group; B is the experimental group)
Detailed Description
In order to show technical solutions, purposes and advantages of the present invention more concisely and clearly, the technical solutions of the present invention are described in detail below with reference to specific embodiments. The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified. The Azospirillum meliloti (Azospirillum melinis) CGMCC1.5340 and the hydrogenotrophus flavus (Hydrogenophaga flava) CGMCC1.8793 in the following examples are purchased from China general microbiological culture Collection center (CGMCC), and the biological material of the present invention can be purchased from CGMCC by the public.
Example 1 preparation of microbial Agents
The embodiment provides a preparation method of a compound microbial preparation, which comprises the following specific steps:
1. preparation of Azospirillum melissii (Azospirillum melinis) CGMCC1.5340 fermentation liquor: inoculating azospirillum melitensis into nitrogen-free liquid culture medium, culturing at 30 deg.C for 48 hr to obtain bacterial suspension, and performing amplification culture until the thallus concentration is not less than 106mL, secondary cultures were obtained. 1L of the medium contained 8g of mannitol, 0.5g of dipotassium hydrogen phosphate, 0.3g of magnesium sulfate, 0.5g of sodium chloride, 0.2g of calcium sulfate and 6g of calcium carbonate, and the pH value was 7.2. + -. 0.2.
2. Preparation of hydrogenotrophus flavus (Hydrogenophaga flava) CGMCC1.8793 fermentation broth: inoculating the hydrogenotrophic yellow bacteria into R2A liquid culture medium, shake culturing at 30 deg.C for 48 hr to obtain bacterial suspension, and performing amplification culture until the thallus concentration is not less than 106mL, secondary cultures were obtained. 1L of the medium contained 0.6g of yeast, 0.25g of tryptone, 0.75g of peptone, 0.5g of glucose, 0.5g of starch, 0.2g of dipotassium phosphate, 0.025g of magnesium sulfate, 0.3g of sodium pyruvate, and pH 7.2. + -. 0.2.
3. And (3) mixing the fermentation liquor obtained in the step (1) and the fermentation liquor obtained in the step (2) according to the volume ratio of 1:2 to prepare the composite microecological bactericide.
The proportions of the composite microbial preparations prepared in examples 1 to 4 and comparative examples 1 to 5 are shown in table 1:
table 1:
| group of | The volume ratio of azospirillum meliloti to hydrogenotrophic bacteria |
| Example 1 | 1:2 |
| Example 2 | 1:3 |
| Example 3 | 1:4 |
| Example 4 | 1:5 |
| Comparative example 1 | 1:1 |
| Comparative example 2 | 1:6 |
| Comparative example 3 | 3:1 |
Note: the only difference between comparative examples 4 and 5 and example 2 is the absence of Azospirillum meliloti and Hydrogen-loving organisms respectively, but the total amount in comparative examples 4 and 5 is the same as in example 2.
Comparative example 4
The only difference between comparative example 4 and example 2 is the absence of Azospirillum meliloti, but the total amount is the same as in example 2.
Comparative example 5
Comparative example 5 differs from example 2 only in the absence of hydrogenotrophus xanthus, but in the same total amount as in example 2.
Comparative example 6
The only difference between comparative example 6 and example 2 is the replacement of the hydrogenotrophus xanthus (hydrogenotropga flava) CGMCC1.8793 with hydrogenotrophus pratensis (hydrogenotropga palleronii) CGMCC 1.12500.
Comparative example 7
Comparative example 7 is different from example 2 only in that atypical hydrogenotrophus (Hydrogenophaga typica) CGMCC 1.13009 replaces hydrogenotrophus xanthus (Hydrogenophaga flava) CGMCC 1.8793.
Comparative example 8
The only difference between comparative example 8 and example 2 is the replacement of Azospirillum melinii (Azospirillum melinis) CGMCC 1.10379 for Azospirillum melinii (CGMCC 1.5340).
Comparative example 9
Comparative example 9 differs from example 2 only in that Azospirillum melitensis (Azospirillum melinis) CGMCC1.8545 is substituted for Azospirillum melinii CGMCC 1.5340.
The total viable count of examples 1 to 4 and comparative examples 1 to 9 is shown in table 2:
table 2:
test example 1
Experiments on corn growth promoting effect of microbial agents prepared in examples 1-4 and comparative examples 1-5
1. The seed germination experiment specifically comprises the following test contents:
the variety of the test crop: zhengdan 958 made from corn
The test method comprises the following steps:
pretreatment: soaking the seeds in 38 deg.C warm water for 1 hr, taking out, treating with 75% ethanol for 1 min, washing with sterile water, soaking with sodium hypochlorite for 45 s, and washing with sterile water for several times.
Grouping experiments: the pretreated seeds are respectively soaked in an experimental group and a control group for 8 hours, the microbial inoculum of each of examples 1-4 and comparative examples 1-5 is used in the experimental group, and the control group is clear water (n is 3). Then, the seeds are put in a dark place at 28 ℃ for accelerating germination for two days, the seeds are continuously grown and cultured under the illumination condition, the growth conditions of the bud length and the root length of the corn are observed after two weeks, and the average value is taken.
The results of the experiment are shown in table 3:
TABLE 3
In table 3, it can be seen from examples 1 to 4 and comparative examples 4 and 5 that the complex microbial inoculum compounded by the honey-yellow hydrogenotrophic bacteria and the yellow hydrogenotrophic bacteria can shorten the germination time of the corn seeds and promote the growth of the sprouts and the development of root systems, while the microbial inocula prepared separately from the complex microbial inocula have no influence on the germination development of the corn seeds, the effect of the complex microbial inocula is similar to that of a control group, and the compounding of the honey-yellow hydrogenotrophic bacteria and the yellow hydrogenotrophic bacteria produces a synergistic effect; as can be seen from comparison of comparative examples 1-3 and examples 1-4, the compounding of the honey-yellow hydrogenotrophic bacteria and the hydrogen xanthovorax bacteria not only produces a synergistic effect, but also the proportion of the two is an important factor influencing whether the composite strains can produce the synergistic effect, and if the proportion exceeds the specific proportion of the two, the effects of promoting seed germination and promoting seed growth cannot be achieved; as can be seen from comparison of comparative examples 6-9 and example 2, the purpose of promoting seed germination and growth can be achieved only by compounding Azospirillum melitensis (CGMCC 1.5340) and hydrogenotrophus xanthus (hydrogenotrophus flava) CGMCC1.8793, and the purpose of the invention can not be achieved if other strains are used.
2、The corn growth promoting experiment specifically comprises the following test contents:
zhengdan 958 is planted in Xin county Tai and Zhen lan Wanshou areas in Qing Yuan City of Guangdong, and planted in a small area in a greenhouse, and phosphate fertilizer, potassium fertilizer and zinc sulfate borax are used as base fertilizers, and the seedling fertilizers are applied and used up before corn topping. The nitrogen fertilizer is applied in stages, 40 percent of the nitrogen fertilizer is used as a base fertilizer, and 60 percent of the nitrogen fertilizer is topdressed in 11-12 leaf development and filling periods. Control group did not administer microbial agent; the microbial inoculum of example 2 was applied to the experimental group, and the microbial inoculum was sprayed three times onto the corn roots in the late seedling stage and the adult stage, respectively.
The contrast group is applied with 15 kg/mu of nitrogenous fertilizer, 5 kg of phosphate fertilizer, 6 kg/mu of potash fertilizer, 1 kg/mu of zinc sulfate and 0.5 kg/mu of borax; the treatment group is applied with 7 kg/mu of nitrogenous fertilizer, 5 kg of phosphate fertilizer, 6 kg/mu of potash fertilizer, 1 kg/mu of zinc sulfate and 0.5 kg/mu of borax.
The results are shown in fig. 1-4, and through observation of paraffin sections, the stalks of the experimental group are tough and strong, and it can be seen that the stalks of the treatment group are full in tissue, high in strength and capable of resisting lodging. The experimental group has the advantages of large number of vascular bundles, large number of cell layers, compact cell arrangement and more storage substances, and the mixed microbial agent can increase the strength and toughness of the stems of the corns and increase the lodging resistance. Through paraffin section observation, the main leaf vein and the leaves of the corn in the experimental group are compared and are thick, and in the vascular bundle sheath cells of the corn, the corresponding leaf vein (containing the vascular bundle sheath cells) and mesophyll tissues (containing the mesophyll cells) are more and firmer than those in the control group, the cells are more and more closely arranged, and the stored substances are more. Through paraffin section observation, the number of corn vascular bundles in an experimental group is large, the number of cell layers is large, cells are arranged tightly, the ground can be firmly grasped, the nutrition absorption area can be increased, and the lodging resistance effect is achieved.
Test example 2 anti-insect action of Complex microbial Agents of the present invention
The test varieties are as follows: zhengdan 958
The application method comprises the following steps: 100mL of the microbial agent prepared in the examples 1-4 and the comparative examples 1-5 and 20L of water are uniformly mixed and then sprayed on the leaf surfaces of crops.
The test method comprises the following steps: according to the corn insect resistance identification technical specification, part 5: the technical specification of the identification of the corn borer resistance is implemented. The results of the experiment are shown in table 4:
table 4:
in table 4, it can be seen from examples 1 to 4 and comparative examples 4 and 5 that the complex microbial inoculum compounded by the honey-yellow hydrogenotrophs and the yellow hydrogenotrophs can improve the resistance of the corn to the corn borers, while the microbial inocula prepared respectively and independently have no insect-resistant effect, and the effect is similar to that of a control group, so that the compounding of the honey-yellow hydrogenotrophs and the yellow hydrogenotrophs generates a synergistic effect, and the resistance to the corn borers is improved; as can be seen from comparison of comparative examples 1-3 and examples 1-4, the compounding of the honey-brown hydrogenotrophic bacterium and the brown hydrogenotrophic bacterium not only produces a synergistic effect, but also the proportion of the honey-brown hydrogenotrophic bacterium and the yellow hydrogenotrophic bacterium is an important factor influencing whether the composite strain can produce the synergistic effect, and if the proportion exceeds the specific proportion of the honey-brown hydrogenotrophic bacterium and the yellow hydrogenotrophic bacterium, the insect-resistant effect can not be realized; as can be seen from comparison of comparative examples 6-9 and example 2, the purpose of improving the resistance of corn to corn borers can be realized only by compounding Azospirillum melinii (CGMCC 1.5340) and hydrogenotrophus xanthus (hydrogengenophaga flava) CGMCC1.8793, and the purpose of the invention cannot be realized if other strains are used.
Table 5 shows Chinese agricultural industry Standard NY/T1248.5-2006, part 5 of the technical Specification for identifying disease and insect resistance of corn: the grading standard of the degree of damage of the corn borers to the heart leaves in the technical specification of the identification of the corn borers.
TABLE 5
| Grade of eating leaves | Description of the symptoms |
| 1 | Only individual blade is provided with 1-2 wormholes with the aperture less than or equal to 1mm |
| 2 | Only individual blade is provided with 3-6 wormholes with the aperture less than or equal to 1mm |
| 3 | A small number of leaf blades are provided with 7 wormholes with the aperture less than or equal to 1mm |
| 4 | 1 to 2 wormholes with the aperture less than or equal to 2mm are arranged on individual blades |
| 5 | A small number of leaf blades are provided with 3-6 wormholes with the aperture less than or equal to 2mm |
| 6 | Part of the leaves are provided with 7 wormholes with the aperture less than or equal to 2mm |
| 7 | The small number of the leaf blades are provided with 1-2 wormholes with the aperture diameter larger than 2mm |
| 8 | Part of the blades are provided with 3-6 wormholes with the aperture diameter larger than 2mm |
| 9 | Most of the blades have 7 wormholes with the aperture larger than 2mm |
Test example 3
Verification of the inventive bacterium pair H2The absorption function is as follows: 100ml of the inoculum prepared in example 1 are taken in a closed tube and subjected to initial H by gas chromatography2Concentration and after 3 days H2To calculate the absorption H2The size of the capability. The results are shown in Table 6:
TABLE 6
Test example 4
The nitrogen fixation activity of the microbial inoculum is verified as follows: 100ml of the microbial inoculum prepared in example 1 was placed in a closed vessel, then acetylene gas was injected to a final concentration of 10%, the vessel was sealed with a medical tape, and after three days of further culture, 100. mu.L of reaction gas was taken, the amount of ethylene produced was measured by a gas chromatograph, and the nitrogenase activity of the strain was calculated according to the formula. Nitrogen fixation activity (nmol/mg. h) ═ C2H4nmol/[ amount of mycoprotein (mg). times.reaction time (h)]Wherein (C)2H4nmol＝1000×C2H4Volume (. mu.l). times.273 XP/[ 22.4 × (273+ t ℃ C.) times.760]Wherein P is the gas pressure (mm Hg) and t is the reaction temperature.
The method for measuring the mycoprotein content comprises the following steps of collecting the thallus in the fermentation liquor, adding 3mL0.5M NaOH into the thallus, boiling the thallus in boiling water for 5min, adding 3mL0.5M HCl, mixing, centrifuging, taking 1.0mL of supernatant, adding 5mL of Coomassie brilliant blue solution, mixing on a vortex mixer, developing for 3 min, measuring the light absorption value A595 at 595nm, and calculating the mycoprotein content according to a bovine serum albumin standard curve.
The result shows that the nitrogen fixation activity of the composite microbial inoculum is 35.12nmol C2H4h.mg protein.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. The compound microbial agent is characterized in that the microbial agent is prepared by mixing fermentation liquor of nitrogen fixing bacteria and hydrogen hydroxide bacteria, wherein the nitrogen fixing bacteria are Azospirillum melitensis (CGMCC 1.5340) and hydrogenotrophic yellow bacteria (hydrogenotropga flava) CGMCC 1.8793.
2. The compound microbial inoculant according to claim 1, wherein the volume ratio of the fermentation broth of azospirillum meliloti to the fermentation broth of hydrogenotrophus flavus is 1: 2-5.
3. The complex microbial inoculant according to claim 1, wherein the volume ratio of the fermentation broth of azospirillum melitensis to the fermentation broth of hydrogenotrophus flavus is 1: 3.
4. The complex microbial inoculant according to claim 1, wherein the viable count in the mixed fermentation broth is 1.3 x 108～2.5×1010One per ml.
5. The use of a microbial inoculant according to any one of claims 1 to 4 as a plant growth promoter.
6. The use of claim 5, wherein the plant is maize.
7. The use of the microbial inoculant of any one of claims 1-4 in a corn anti-pest formulation.
8. The use of claim 7, wherein said pest resistance is corn borer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110152711.0A CN112852671B (en) | 2021-02-04 | 2021-02-04 | Microbial preparation for promoting corn growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110152711.0A CN112852671B (en) | 2021-02-04 | 2021-02-04 | Microbial preparation for promoting corn growth |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112852671A true CN112852671A (en) | 2021-05-28 |
| CN112852671B CN112852671B (en) | 2023-10-20 |
Family
ID=75986539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110152711.0A Active CN112852671B (en) | 2021-02-04 | 2021-02-04 | Microbial preparation for promoting corn growth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112852671B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940969A (en) * | 2021-02-07 | 2021-06-11 | 兴安盟莱绅生物农业有限公司 | Novel microbial agent and soybean planting method |
| CN113046260A (en) * | 2021-02-04 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Microbial mixed microbial inoculum for promoting soybean growth and application thereof |
Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102976840A (en) * | 2011-09-05 | 2013-03-20 | 麻林涛 | Specialized composite microbial fertilizer for corn, and preparation method thereof |
| EP2944697A1 (en) * | 2014-05-13 | 2015-11-18 | Evonik Degussa GmbH | Method of producing nylon |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿***料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
| WO2018213568A1 (en) * | 2017-05-17 | 2018-11-22 | President And Fellows Of Harvard College | Biofertilzer and methods of making and using same |
| CN110627209A (en) * | 2019-10-18 | 2019-12-31 | 中国科学院水生生物研究所 | Iron-coupled autotrophic denitrification system and method for removing nitrate in wastewater with low carbon-nitrogen ratio |
| CN110678539A (en) * | 2017-02-03 | 2020-01-10 | 基沃德股份有限公司 | CO2And other C1 substrates into pure nutrients, fertilizers, biostimulants and systems for accelerating carbon sequestration in soils |
| CN112481159A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial agent and application thereof |
| CN112501061A (en) * | 2020-11-27 | 2021-03-16 | 广东丽豪生物农业有限公司 | Microbial agent for wheat planting |
| CN112940969A (en) * | 2021-02-07 | 2021-06-11 | 兴安盟莱绅生物农业有限公司 | Novel microbial agent and soybean planting method |
| CN112980732A (en) * | 2021-03-17 | 2021-06-18 | 广东丽豪生物农业有限公司 | Microbial agent for promoting growth of rape and rape planting method thereof |
| CN113046260A (en) * | 2021-02-04 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Microbial mixed microbial inoculum for promoting soybean growth and application thereof |
| CN113040175A (en) * | 2021-02-07 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Oat seed coating agent for pseudomonas mendocina |
| CN113121284A (en) * | 2021-05-06 | 2021-07-16 | 广东丽豪生物农业有限公司 | Composite organic bacterial fertilizer and preparation method and application thereof |
| CN113215028A (en) * | 2021-03-30 | 2021-08-06 | 广东丽豪生物农业有限公司 | Microbial flora and microbial agent and application thereof |
| CN113337420A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Composite nitrogen-fixing microbial agent and preparation method and application thereof |
| CN113337421A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Compound microbial agent and application thereof |
| CN114231465A (en) * | 2021-12-30 | 2022-03-25 | 广东丽豪生物农业有限公司 | Microbial preparation for improving iron deficiency stress resistance of crops and application thereof |
-
2021
- 2021-02-04 CN CN202110152711.0A patent/CN112852671B/en active Active
Patent Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102976840A (en) * | 2011-09-05 | 2013-03-20 | 麻林涛 | Specialized composite microbial fertilizer for corn, and preparation method thereof |
| EP2944697A1 (en) * | 2014-05-13 | 2015-11-18 | Evonik Degussa GmbH | Method of producing nylon |
| CN110678539A (en) * | 2017-02-03 | 2020-01-10 | 基沃德股份有限公司 | CO2And other C1 substrates into pure nutrients, fertilizers, biostimulants and systems for accelerating carbon sequestration in soils |
| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿***料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
| WO2018213568A1 (en) * | 2017-05-17 | 2018-11-22 | President And Fellows Of Harvard College | Biofertilzer and methods of making and using same |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
| CN110627209A (en) * | 2019-10-18 | 2019-12-31 | 中国科学院水生生物研究所 | Iron-coupled autotrophic denitrification system and method for removing nitrate in wastewater with low carbon-nitrogen ratio |
| CN112501061A (en) * | 2020-11-27 | 2021-03-16 | 广东丽豪生物农业有限公司 | Microbial agent for wheat planting |
| CN112481159A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial agent and application thereof |
| CN113046260A (en) * | 2021-02-04 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Microbial mixed microbial inoculum for promoting soybean growth and application thereof |
| CN112940969A (en) * | 2021-02-07 | 2021-06-11 | 兴安盟莱绅生物农业有限公司 | Novel microbial agent and soybean planting method |
| CN113040175A (en) * | 2021-02-07 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Oat seed coating agent for pseudomonas mendocina |
| CN112980732A (en) * | 2021-03-17 | 2021-06-18 | 广东丽豪生物农业有限公司 | Microbial agent for promoting growth of rape and rape planting method thereof |
| CN113215028A (en) * | 2021-03-30 | 2021-08-06 | 广东丽豪生物农业有限公司 | Microbial flora and microbial agent and application thereof |
| CN113121284A (en) * | 2021-05-06 | 2021-07-16 | 广东丽豪生物农业有限公司 | Composite organic bacterial fertilizer and preparation method and application thereof |
| CN113337420A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Composite nitrogen-fixing microbial agent and preparation method and application thereof |
| CN113337421A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Compound microbial agent and application thereof |
| CN114231465A (en) * | 2021-12-30 | 2022-03-25 | 广东丽豪生物农业有限公司 | Microbial preparation for improving iron deficiency stress resistance of crops and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| ARTURO ABURTO等: "Degradation of a benzene–toluene mixture by hydrocarbon-adapted bacterial communities", ANN MICROBIOL * |
| 刘华伟;林晓军;孙超;***;杨呼;郭蔼光;: "接种两种固氮菌增强小麦幼苗抗渗透胁迫及生长能力", 植物生态学报 * |
| 王亚楠;陈莹莹;吴玉洪;吴海霞;暴增海;马桂珍;: "甲基营养型芽孢杆菌对黄瓜促生作用及其机理研究", 北方园艺 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113046260A (en) * | 2021-02-04 | 2021-06-29 | 兴安盟莱绅生物农业有限公司 | Microbial mixed microbial inoculum for promoting soybean growth and application thereof |
| CN113046260B (en) * | 2021-02-04 | 2023-04-25 | 兴安盟莱绅生物农业有限公司 | Microbial mixed microbial agent for promoting soybean growth and application thereof |
| CN112940969A (en) * | 2021-02-07 | 2021-06-11 | 兴安盟莱绅生物农业有限公司 | Novel microbial agent and soybean planting method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112852671B (en) | 2023-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2514815B1 (en) | Biocontrol strain for cucumber and watermelon continuous cropping wilt diseases and microbial organic fertilizer thereof | |
| CN112501061B (en) | Microbial agent for wheat planting | |
| CN112481159B (en) | Microbial agent and application thereof | |
| CN112852671B (en) | Microbial preparation for promoting corn growth | |
| CN108029501A (en) | A kind of vegetable nutrient diseases prevention seedling medium and preparation method and application | |
| CN116463256B (en) | Composite microbial agent and preparation method and application thereof | |
| CN110463551B (en) | Biological control method for peanut root knot nematode disease | |
| CN106927948A (en) | A kind of medicine fertilizer and its application | |
| CN112481161A (en) | Microbial agent for promoting wheat growth and wheat breeding method thereof | |
| CN116671408A (en) | Lily high-yield and high-quality cultivation method | |
| İşçi et al. | Effects of IBA and plant growth-promoting rhizobacteria (PGPR) on rooting of Ramsey American grapevine rootstock. | |
| CN107032918A (en) | A kind of few core slides the implantation methods of skin cumquat | |
| JPH10251087A (en) | Organic fermented fertilizer and its use | |
| CN111471620B (en) | Biocontrol bacterium BV03, application thereof, microbial inoculum and preparation method of microbial inoculum | |
| CN112430554B (en) | Biocontrol bacterium BTh05 for damping-off of melons and fruits, microbial inoculum thereof, preparation method of microbial inoculum and application of microbial inoculum | |
| KR20090095149A (en) | Fertilizer composition containing natural plant vinegar | |
| JPS6024075B2 (en) | Method for manufacturing organic fertilizer ripening agent | |
| CN103275892A (en) | Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes | |
| CN113396663A (en) | Crop high-yield regulation and control method in microbial fertilizer and straw returning deep ploughing mode | |
| CN112806138A (en) | Method for improving cold resistance of rice based on complete decomposition and returning of rice straw to field | |
| CN115232764B (en) | Microbial agent | |
| CN111727847A (en) | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method | |
| CN110903136A (en) | Microbial fertilizer and preparation method thereof | |
| Sukmaningrum et al. | Response of Microorganism Suspension and Various Kinds of Organic Fertilizers to the Development of Pathogen Fusarium sp. on Shallots | |
| NL2032380B1 (en) | Soil conditioner and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |