CN1127913C - Nutrient health-care food with immunocompetence improvement - Google Patents

Nutrient health-care food with immunocompetence improvement Download PDF

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CN1127913C
CN1127913C CN00129906A CN00129906A CN1127913C CN 1127913 C CN1127913 C CN 1127913C CN 00129906 A CN00129906 A CN 00129906A CN 00129906 A CN00129906 A CN 00129906A CN 1127913 C CN1127913 C CN 1127913C
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nutrient
powder
glossy ganoderma
soybean
sea
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CN00129906A
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CN1287800A (en
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赵钰凤
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BEIJING LONG-LIFE HEALTH-CARE PRODUCTS Co Ltd
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BEIJING LONG-LIFE HEALTH-CARE PRODUCTS Co Ltd
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Abstract

The present invention relates to a nutrient health care product for regulating immune functions. The present invention is a capsule or a tablet made by the following steps: power is prepared from the fine powder extracted from lucid ganoderma, soybean and seatangle by the enzymolysis technique of complex phosphoesterasum; the power is scientifically processed and mixed to form capsules or tablets. The present invention has not only abundant nutrient substances, but also significant physiological activators such as at least 20 to 40 milligrams/grams of total saponins because of a dietotherapeutic characteristic and is easily digested and absorbed because of good solubility of nutrient ingredients. The nutrient health care product of the present invention can obviously increase the body fluid of the organism and improve the phagocytosis of the macrophage; the present invention is a nutrient health care product with the function of immunity regulation to improve the immunity and physiological effect.

Description

A kind of nutrient and healthcare products of regulating immunologic function
The present invention relates to a kind of nutrient and healthcare products and manufacture method thereof, particularly a kind of have the adjusting immunologic function and engulf the macrophage nutrient and healthcare products and the manufacture method of employing complex enzyme hydrolysis technology.
As everyone knows, after the mankind step into old-age group, the various physiological functions of himself fail gradually, have a delicate constitution, especially immunologic function is along with age growth is constantly low, resist external also corresponding the weakening of various germ infiltration capabilities that causes, the elderly's disease is increased, this is the law of development of human society.From ancient times to the present, people look forward to always, and strengthen everyone physique, raising immunocompetence, the generation of minimizing disease, thereby life-saving by every possible means.The past people like green plants albumen one soybean of diet always, because soybean molecular structure complexity, dissolubility is low, influences digestibility and biologicak efficiency, and the back that is difficult to be eaten influences actual value for human body institute all absorbs.Entered since the seventies and eighties, people are aspect exploitation soybean deep-processed food, mainly the soybean protein deep processing is become easy absorbed polypeptide, adopt trypsase or pepsin, but the animal protein enzyme source is limited, and price is also expensive, so still generally adopt hay bacillus 1398 etc., put a bacterium 166 and carry out enzymolysis, produce and to have the polypeptide soy food product of bitter taste and peculiar smell in various degree.
The flat 4-53460 of Japan discloses a kind of with endopeptidase and endoglucanase fermentation dipping soybean, mixes being used for improving diabetes then with spirulina powder, essential oil or glossy ganoderma powder, the functional food of low blood pressure and insomnia usefulness.
The objective of the invention is to through the inventor nutrition developmental researches for many years such as soybean, overcome the defective of present prior art, adopt up-to-date complex enzyme zymohydrolysis technology to combine, a kind of nutrient and healthcare products and manufacture method thereof of regulating immunologic function and engulfing macrophage that have of eating medicine nutrition overall balance, complementation is provided with traditional Chinese medicine.
The nutrient and healthcare products of adjusting immunologic function provided by the invention comprise following raw materials according component and percentage by weight thereof,
Soybean 40-70
Sea-tangle 5-20
Glossy ganoderma 10-40
Be preferably soybean 50-65, sea-tangle 10-15, glossy ganoderma 20-35.More preferably soybean 60, sea-tangle 10, glossy ganoderma 30.
In the nutrient and healthcare products according to adjusting immunologic function provided by the invention, described raw soybeans sea-tangle is through the contain mixtures of polypeptides of complex enzyme technology through enzymolysis.Well-known soybean is human main plant nutrient protein source, contain rich in protein, multiple unrighted acid, vitamin, mineral matter, also have important physical active material such as saponin, isoflavone, for example per 100 gram soybean contain 16 gram protein, 7.8 gram carbohydrates, 9 gram lipids and 2.1 gram celluloses and other a large amount of nutritions etc.According to modern nutriology research report, soybean molecular structure complexity, form solid spherical structure, irregular structure accounts for 61%, α-Luo Xuanjiegou accounts for 5-6%, beta structure accounts for 34-35%, beta structure is antiparallel beta structure, and to be that the random structure of globulin molecule is partially folded be very solid tertiary structure, and then the complicated quaternary structure of formation, this extremely compact texture is decomposed enzyme must have very strong resistivity, make poorly solublely simultaneously, influence digestibility and biological effect, the back absorption rate is low so soybean protein is eaten.The inventor uses up-to-date compound bio enzyme resolving tech, it is complementary to adopt plurality of enzymes to cooperate, and makes soybean and the sea-tangle enzymolysis that ferments together, destroys to be difficult to be become the micromolecule polypeptide mixture by the protein structure that people digest and assimilate, to be lower than 1000, common 300-700, and based on two third-class low molecular peptides, its amino acid is formed almost completely the same with soybean protein, make it have unique functional and nutritive peculiarity, dissolubility is good, and the digestibility height is easily directly absorbed by people.Mixtures of polypeptides is best in the absorptivity of enteron aisle, and the modern medicine proof has the effect of obvious reduction cholesterol.Soyasaponins has the lipid peroxide of reduction to generate, thereby reduces or the elimination free radical, suppresses the damage of radical pair cell membrane, and the protection cell membrane is avoided damage, improves body metabolism, strengthens immunocompetence.The isoflavones material, it 2/3rds is a tri hydroxy isoflavone, it be normal cell and tumor information transmit enzyme inhibitor arranged by force, and the anti-significantly anti-multiple effect that lures is arranged.Soybean is not only improved nutritive value through the complex enzyme hydrolysis afterproduct, easily is absorbed, and can makes full use of these special compositions, the performance physiological active functions.Soybean varieties is not particularly limited in nutrient and healthcare products of the present invention, and yellow, green, black all can use, and preferably uses the Northeast China soybean; Soybean consumption (by weight percentage) is generally 40-70, is preferably 50-65, and more preferably 60.
In nutrient and healthcare products provided by the invention, described sea-tangle is not particularly limited, and preferably with Chinese Shandong coastal product sea-tangle, it is after marine collection, removes impurity, the dried sea-tangle after drying in the shade with fresh water is clean.Common water content 28.5%, ash 17%, crude fibre 21.2%, nitrogen 0.71%, protein 4.81%, fat 1.2%, pentosan 8.82% reaches abundant iodine etc.Carry out complex enzyme hydrolysis with soybean during use, make compositions such as original sea-tangle composition such as protein, cellulose, carbohydrate become the easy absorbed material of solubility, sea-tangle glycan in the sea-tangle can reduce cholesterol, and Soluble Fiber have prevention tumour and heavy metal poisoning function.The general by weight percentage 5-20 of sea-tangle consumption in nutrient and healthcare products is preferably 10-20, and more preferably 10.In nutrient and healthcare products provided by the invention, described glossy ganoderma is the smart powder that extracts through water retting.Glossy ganoderma is the famous and precious tonic herb of China's tradition, puts down in writing the title of " lucid ganoderma " in Chinese Compendium of Materia Medica for a long time, is a kind of edible mushroom.Through the modern medicine clinical testing, chemical composition analysis and pharmacological action etc. studies confirm that, glossy ganoderma is that a kind of building up one's health by taking tonic kept fit, strengthen the body resistance to consolidate the constitution, the Chinese medicine of promoting longevity contains abundant effective pharmacological active substance, can improve body immunity as GL-B, eliminate free radical, suppress the anti-radiation of tumour; Ganoderic acid is a kind of triterpene substance, has calmness to protect the liver, the killing tumor cell function; Adenosine has the microcirculation of improvement, improves the oxygen delivery capacity to heart and brain; Contain needed by human body Ca, Mg, Fe, Se, P, Zn, Ge etc. simultaneously, especially Ge, Se, Mg have anticancer, anti-senescence function.Modern age, medical research proof glossy ganoderma was a kind of immunomodulator.The dipping powder of ganoderma lucidum fruitbody has all effective ingredients and the active material of former glossy ganoderma, and contains 20 several amino acids of needed by human body; Its composition is more reasonable, and content is also abundant.
Described glossy ganoderma can be used Wild ganoderma or cultivation glossy ganoderma, preferably uses Wild ganoderma, but because the Wild ganoderma source is limited, adopts cultivation glossy ganoderma, for example red ganoderma usually.Glossy ganoderma consumption in nutrient and healthcare products is generally 10-40 by weight percentage, is preferably 20-35, and more preferably 30.
The production method of nutrient and healthcare products provided by the invention comprises the following steps:
1, will be milled to 60 purpose powders respectively through raw soybeans and sea-tangle selected, that clean, dry, the common 50-90 of described bake out temperature ℃, preferred 80 ℃.
2, the powder that step 1 is obtained adds thermal agitation 20-60 minute with water by the material component percentage by weight in jar fermenter, and preferred 30 minutes, powder and water weight ratio were 1: 5 to 1: 20, preferably are taken as 1: 10; Temperature is 60-95 ℃, is preferably 90 ℃, is chilled to 50 ℃ then.
3, be chilled to 50 ℃, add 1% (weight) by cellulase, pectase and papain with the complex enzyme formed at 1: 1: 1 in PH5-6,50 ℃ of temperature bottom fermentation enzymolysis 5-8 hour are preferably 6 hours.
4, the fermentation enzymolysis material of step 3 is removed residue after filtration, cross 100 mesh sieve powder after concentrating filter liquor, the spray-drying.Described thickening temperature is 50-60 ℃.
5, glossy ganoderma powder is broken into 20 order coarse granules, soaked earlier 1-3 hour, after be heated to 80-90 ℃ and kept the filtrate immersion liquid 2-3 hour, repeat three times, merging filtrate is through centrifugal-film evaporator, be concentrated into 1/3rd,, make smart powder again through the high speed centrifugation duster;
6, the refining powder lot that step 4 and 5 is obtained mixes, and back filled capsules or make tablet is packaged into finished product behind illumination-based disinfection.
In the manufacture method of described nutrient and healthcare products of the present invention, wherein said complex enzyme is meant that cellulase, pectase and papain are composite and forms that three kinds of enzymes can adopt any commercially available enzyme, for example:
Cellulase: the biochemical east wind biochemical corp in Chinese Academy of Sciences Shanghai is produced,
Production code member: 4104, every gram contains more than filter paper enzyme activity 15000 units.
Buy to Medical Depot.
Pectase: Hongshan Biochemical Industry Co., Fuzhou City produces, the product approval number:
No. the 041st, Min Wei food word (1994), product assay certificate number: newly reflect No. 022, word of 93 Fujian sections, buy enzyme activity 〉=20,000 M/g to producer.
Papain: the product of thing enzyme preparation factory takes place in Wuxi enzyme preparation factory or chemical engineering institute of Guangxi University sea, and enzyme activity is 85-125M/g.
Three kinds of enzyme-cellulose enzymes: pectase: the composite ratio of papain is 1: 1: 1.Fermentation during enzymolysis in jar fermenter bubbling air, guarantee the enzymolysis requisite oxygen, Ventilation Rate is decided on production scale.Fermentation enzymolysis PH is 5-6.
The smart powder of described glossy ganoderma is to select clean Wild ganoderma or cultivation glossy ganoderma such as artificial red ganoderma for use, and preferred Wild ganoderma was soaked 1-3 hour through being ground into 20 order coarse granules, preferred 2 hours, be heated to 80-90 ℃ after the immersion and kept 2-3 hour, preferred 2 hours, the filtrate immersion liquid, repeat three times, merging filtrate through centrifugal-film evaporator, is concentrated into 1/3 liquid, through the high speed centrifugation duster, make smart powder again.
Described finished product is a light brown powder, and evenly disperseing does not have caking, has distinctive smell and flavour.Have food, medicine has both characteristics, not only contain abundant nutrient, and the important physical active material is arranged, soybean and sea-tangle are by behind the enzymolysis, it is good to make relevant nutrition become dissolubility, human body easily digests, the absorptivity height, contain after testing total saponin (〉=20-40mg/g), polysaccharide (10-15mg/g) and a large amount of protein, Cobastab 1, B 2, C, mineral matter and microelements of calcium, phosphorus, iron, manganese, zinc, selenium and more than ten plants human amino acid needed.Experimental results show that and have the effect that significantly improves body, body fluid and macrophage phagocytic, suppress the lipid peroxidation deposits yields, eliminate free radical, strengthen immunity of organisms, improve metabolism, regulate total balance of the body, can bring into play complementary, synergy, the inhibition fat absorption be arranged, promote lipolysis as the saponin in the soya bean, stop the lipid peroxidation deposits yields, but can make body row iodine, and containing abundant iodine in the sea-tangle, both cooperations can be removed disadvantage from, more can improve the nutritive effect of product, the performance health-care effect comprehensively.
Bring into play the characteristics that three kinds of bulk drug tablewares are equipped with and the characteristic of nutrition and function thereof, take for a long time, can reach nutrient balance, improve the quality of living, improve immunity, the generation of pre-preventing tumor and physiology degenerative disorders.The qualifications and record of service source of these three kinds of raw materials is abundant, and carrying out deep processing development production is health nutrient, and physiologic effect is not only arranged, and also has favorable economic benefit.
The present invention further specifies the present invention with the following example, but the present invention is not limited to embodiment, does some modification those of ordinary skills, still in protection domain of the present invention.
Embodiment 1
One, glossy ganoderma is extracted the manufacturing of smart powder
Red ganoderma 2.5Kg with cultivation, drop in the container through being ground into 20 order coarse grain, soak 2 hours (amount of water is as the criterion with the submergence ganoderma particles), be heated to 90 ℃ then and kept 2 hours, filter out filtrate, filter residue is soaked in water again, triplicate, merging filtrate, filtrate is concentrated into 1/3rd liquid through centrifugal-film evaporator, through the high speed centrifugation duster, get smart powder again.
Two, soybean sea-tangle enzymolysis product is made
Selected, clean with carrying out respectively, wear into 60 purpose powders in the soybean 6.7Kg of 80-85 ℃ of oven dry and sea-tangle 0.8Kg and drop in the jar fermenter, add 100Kg water, stirred 0.5 hour in 90 ℃, be cooled to 50 ℃ then.Add the complex enzyme of raw material 1% (weight), is made up of cellulase, pectase and protease, proportioning is 1: 1: 1, and in 50 ℃ of enzymolysis 6 hours of fermenting, enzymolysis PH is 5-6.5.Fermentation enzymolysis material is removed residue after filtration, and filtrate concentrates in 50-60 ℃, after spray-drying again, cross 100 mesh sieves and get smart powder.
Glossy ganoderma extracted smart powder and mix, load capsule (0.35 gram) or make tablet with the smart powder of soybean sea-tangle enzymolysis, again behind the radiation sterilization finished product.
Embodiment 2
Embodiment 2 is identical with the manufacturing step of embodiment 1, and not being both the raw material glossy ganoderma is 3Kg, and sea-tangle is 1Kg.
Under classify Beijing Sanitary and Antiepidemic Station tampon as and survey result's report
The 1# sample The 2# sample The 3# sample
Total plate count (cfu/g) 40 30 50
Coliform MPN (100g) <30 <30 <30
Pathogenic bacteria Do not detect Do not detect Do not detect
Yeast (cfu/g) <5 <5 <5
Mould (cfu/g) <5 <5 <5
Arsenic (mg/Kg) <0.30 <0.30 <0.30
Plumbous (mg/Kg) <0.50 <0.50 <0.50
Copper (mg/Kg) 8.48 8.32 8.61
Ash content (%) 3.5 3.4 3.5
Moisture (%) 8.3 8.2 8.4
Total saponin (mg/Kg) 0.29 0.33 0.2
Adenosine (ug/g) Do not detect Do not detect Do not detect
Polysaccharide (mg/g) 13.0 11.2 13.3
Calcium (mg/100g) 338.19 332.65 328.67
Zinc (mg/100g) 22.98 24.04 25.86
Selenium (ug/g) 16.27 21.56 17.30
Amino acid content (mg/100g)
Asparatate 273.77 266.52 279.21
Glutamic acid 319.49 321.52 321.45
Serine 214.75 229.14 217.68
Glycine 154.75 ?163.58 ?169.65
Histidine 318.59 ?320.34 ?312.42
Arginine 351.85 ?347.45 ?349.70
Threonine 265.71 ?270.53 ?260.90
Alanine 210.78 ?219.53 ?215.62
Proline 279.65 ?281.12 ?276.24
Tyrosine 375.66 ?380.21 ?375.44
Valine 293.34 ?220.89 ?234.54
Methionine 316.98 ?320.36 ?330.21
Cysteine 124.68 ?129.34 ?118.65
Isoleucine 276.89 ?282.86 ?278.77
Leucine 267.90 ?270.41 ?279.12
Phenylalanine 347.92 ?344.35 ?335.32
Lysine 329.10 ?326.21 ?330.51
Protein (%) 40.3 ?40.1 ?40.0
Notice that sample 1-3# is that the raw material that is same as embodiment 2 is formed and weight proportion
Through food section of Beijing Sanitary and Antiepidemic Station through physics and chemistry, Micro biological Tests, all indexs all meet national related food sanitary standard and the requirement of health food general sanitary, constant product quality, shelf-life is 1.5 years, through the acute oral toxicity test result, judge the nontoxic level in the true border of product capsule according to acute grade scale in " foodsafety assessment process of science and method ".
Advance institute's health food Function detection center examining report through Beijing Union University's practical writing, per os give mouse not behind the capsule product of dosage high dose can improve mouse humoral immune function 5.9% (P<0.05), improve macrophage phagocytosis of mice 9.4% (P<0.05).According to the regulation of " the function assessment assessment process and the detection method of health food ", judge that the high dose capsule product has immunoregulation effect.

Claims (8)

1, a kind of nutrient and healthcare products of regulating immunologic function is characterized in that: comprise following raw materials according component and percentage by weight
Soybean 40-70
Sea-tangle 5-20
Glossy ganoderma 10-40
Wherein glossy ganoderma is to extract the smart powder of the glossy ganoderma that obtains through the water logging bubble, soybean and sea-tangle are through selected, clean, oven dry is ground into powder, in jar fermenter, add thermal agitation 20-60 minute with water, powder and water weight ratio 1: 5 to 1: 20, temperature 60-95 ℃, be chilled to 50 ℃, add 1% (weight) by cellulase, the complex enzyme that pectase and papain are formed at 1: 1: 1, in PH5-6,50 ℃ of temperature bottom fermentation enzymolysis 5-8 hour, again after filtration, concentrate, the powder that spray-drying obtains, full and uniform capsule or the tablet of being mixed and made into of the smart powder of powder that spray-drying is obtained and glossy ganoderma.
2, according to a kind of nutrient and healthcare products of regulating immunologic function of claim 1, it is characterized in that: described material component and percentage by weight are soybean 50-65, sea-tangle 10-15, glossy ganoderma 20-35.
3, according to a kind of nutrient and healthcare products of regulating immunologic function of claim 1, it is characterized in that: described material component and percentage by weight are soybean 60, sea-tangle 10, glossy ganoderma 30.
4, a kind of nutrient and healthcare products of regulating immunologic function arbitrary according to claim 1-3 is characterized in that: described glossy ganoderma is natural glossy ganoderma or cultivation red ganoderma.
5, a kind of manufacture method of regulating the nutrient and healthcare products of immunologic function comprises the following steps:
(1) raw soybeans and sea-tangle are carried out respectively selected, clean, oven dry is ground into 60 purpose powders;
(2) powder that step 1 is obtained is by soybean 40-70, and sea-tangle 5-20 percentage by weight adds thermal agitation 20-60 minute with water in jar fermenter, powder and water weight ratio 1: 5 to 1: 20, temperature 60-95 ℃;
(3) be chilled to 50 ℃, add 1% (weight) by cellulase, pectase and papain with the complex enzyme formed at 1: 1: 1 in PH5-6,50 ℃ of temperature bottom fermentation enzymolysis 5-8 hour;
(4) the fermentation enzymolysis material of step (3) is removed residue after filtration, filtrate concentrates, spray-drying is crossed 100 mesh sieve powder, and described thickening temperature is 50-60 ℃;
(5) glossy ganoderma is ground into 20 order coarse granules by the 10-40 percentage by weight, soaked earlier 1-3 hour, after be heated to 80-90 ℃ and kept 2-3 hour, the filtrate immersion liquid repeats merging filtrate three times, through centrifugal-film evaporator, be concentrated into 1/3rd,, make smart powder again through the high speed centrifugation duster;
(6) refining powder lot that step (4) and (5) are obtained mixes, and back filled capsules or make tablet becomes finished product again behind illumination-based disinfection.
6, according to a kind of manufacture method of regulating the nutrient and healthcare products of immunologic function of claim 5, wherein glossy ganoderma is Wild ganoderma or cultivation red ganoderma.
7, according to a kind of manufacture method of regulating the nutrient and healthcare products of immunologic function of claim 5, powder and water weight ratio are 1: 10 in the wherein said step (2).
8, according to a kind of manufacture method of regulating the nutrient and healthcare products of immunologic function of claim 5, wherein said fermentation enzymolysis time is 6 hours.
CN00129906A 2000-10-09 2000-10-09 Nutrient health-care food with immunocompetence improvement Expired - Fee Related CN1127913C (en)

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Publication number Priority date Publication date Assignee Title
CN103070431B (en) * 2012-08-22 2013-11-27 浙江省海洋开发研究院 Kelp extract iodine tablet and preparation method thereof
CN117297096A (en) * 2023-11-29 2023-12-29 吉林省特医食品生物科技有限公司 Medicine and food homologous composition for treating cardiovascular and cerebrovascular diseases and preparation method thereof

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