CN112716839B

CN112716839B - Composition for resisting oxidation and repairing oxidative damage and application thereof

Info

Publication number: CN112716839B
Application number: CN202011545846.5A
Authority: CN
Inventors: 朱翠翠; 鲁呈韬; 李正日; 高宏旗
Original assignee: Shanghai Linqingxuan Biotechnology Co ltd
Current assignee: Shanghai Linqingxuan Biotechnology Co ltd
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2023-01-03
Anticipated expiration: 2040-12-23
Also published as: CN112716839A

Abstract

The invention provides an antioxidant and oxidative damage repairing composition and application thereof, wherein the composition comprises the following components in percentage by mass: 5-50% of water, 20-50% of 1,3-propylene glycol, 0.5-20% of camellia leaf extract, 0.5-20% of camellia extract, 2-20% of panthenol, 2-20% of uric acid, 0.1-5% of epigallocatechin gallate, 0.2-5% of nicotinamide, 0.1-5% of curcumin, 0.1-5% of paeonol, 0.1-5% of caffeine, 0.2-5% of dipotassium glycyrrhizinate and 0.1-5% of silymarin. The formula of the invention contains an antioxidant component for eliminating active oxygen, and is matched with a synergistic component, so that the autophagy component of cells can be further increased, the damage of the active oxygen to the skin is reduced, the self antioxidant capacity of the skin is improved, and the capacity of the skin for repairing oxidative damage is accelerated.

Description

Composition for resisting oxidation and repairing oxidative damage and application thereof

Technical Field

The invention relates to the field of cosmetics, and particularly relates to an antioxidant and oxidative damage repairing composition containing camellia extract and application of the composition. The camellia extract-containing composition for resisting oxidation and repairing oxidative damage disclosed by the invention has the effects of resisting oxidation, promoting cell self-oxidation, repairing oxidative damage, effectively improving the ultraviolet resistance of skin and promoting after-sun repair, and can be used as an effective raw material to be added into cosmetics.

Background

The skin is the largest organ of our human body, is positioned on the surface of our human body, is in contact with the internal environment and the external environment of our human body, and mainly plays a role in protecting our human body. The skin, which is the outermost organ, is exposed to harmful environmental factors such as UV, air pollutants, pathogens, physical and chemical stimuli, etc., and thus is the most vulnerable organ. Reactive Oxygen Species (ROS) play an important role in many processes where many external harmful factors attack the skin. ROS are involved in skin aging and skin wrinkles, as in the reactions of UV-induced skin inflammation. The skin is used as a first line of defense against external harmful factors and is also an important indicator of the personal appearance, so that the effect of harmful factors on the skin is inhibited and eliminated in various ways, which is a problem of cosmetic and medical attention.

Patent document CN111904911a describes a plant antioxidant composition containing notoginseng, which is prepared from the following raw materials in parts by weight: 0.1-50 parts of fresh panax notoginseng freeze-dried tablets, 0.1-50 parts of giant knotweed rhizome, 0.1-50 parts of white plum blossom and 1-60 parts of aloe leaves. The composition has the effects of clearing various free radicals, resisting and repairing oxidative damage and maintaining redox balance. However, the effect of scavenging free radicals is still to be improved.

In view of the foregoing, there is a need to produce compositions that are more effective at scavenging ROS and repairing oxidative damage.

Disclosure of Invention

In order to solve the defects of the prior art, the invention provides a composition of a composition for resisting oxidation and repairing oxidative damage and application thereof. Meanwhile, the composition is applied to products such as skin moistening oil, emulsion, gel, cream and the like, which have the effects of resisting aging, wrinkles and UV, and improving after-sun repair.

The purpose of the invention is realized by the following technical scheme:

the invention provides a composition for resisting oxidation and repairing oxidative damage, which comprises the following components in percentage by mass:

5 to 50 percent of water,

1,3-propylene glycol 20-50%,

0.5-20% of camellia leaf extract,

0.5-20% of camellia extract,

2 to 20 percent of panthenol,

2 to 20 percent of uric acid,

0.1 to 5 percent of epigallocatechin gallate,

0.2 to 5 percent of nicotinamide,

0.1 to 5 percent of curcumin,

0.1 to 5 percent of paeonol,

0.1 to 5 percent of caffeine,

0.2 to 5 percent of dipotassium glycyrrhizinate,

0.1-5% of silymarin.

Preferably, the composition for resisting oxidation and repairing oxidative damage comprises the following components in percentage by mass:

5 to 13 percent of water,

1,3-propylene glycol 20-25%,

camellia japonica leaf extract 0.5-13.5%,

11.5 to 20 percent of camellia extract,

2 to 11.5 percent of panthenol,

10.5 to 20 percent of uric acid,

0.1-4.5% of epigallocatechin gallate,

2 to 5 percent of nicotinamide,

1.5 to 5 percent of curcumin,

2.5 to 5 percent of paeonol,

0.1 to 4 percent of caffeine,

0.2 to 3 percent of dipotassium glycyrrhizinate,

silymarin 2-5%.

In the invention, the camellia leaf extract is a leaf extract of camellia in the family of Theaceae and has the effects of sterilization and anti-inflammation;

the camellia extract can be used for treating burns, scalds and wound bleeding;

the panthenol is also called provitamin B5, is ubiquitous in animals and plants, and has the effects of moisturizing, relieving and repairing;

uric acid needs the assistance of other antioxidants to play antioxidant activity in the organism;

epigallocatechin gallate is a chemical component of plants such as tea, and has repairing and relieving effects;

the nicotinamide is an amide compound of nicotinic acid, has the effects of whitening skin, resisting aging and the like, and is mainly used for preventing and treating pellagra, stomatitis, glossitis and the like clinically;

curcumin is a polyphenol compound extracted and purified from curcuma longa L. medium plants, has wide pharmacological activities of resisting inflammation, resisting oxidation, regulating lipid, resisting virus, resisting infection, resisting tumor, resisting coagulation, resisting hepatic fibrosis, resisting atherosclerosis and the like, and has low toxicity and small adverse reaction;

paeonol is a component extracted from dried root bark of Paeonia suffruticosa Andr of Ranunculaceae, and has effects of relieving pain, resisting inflammation, relieving fever and inhibiting allergic reaction.

Coffee has effects of exciting nerve due to one component of coffee and tea;

the dipotassium glycyrrhizinate is extract of plants such as Glycyrrhiza glabra L, and has anti-inflammatory, antiallergic, and moisturizing effects;

silymarin is extract of plants such as Silybum marianum of Compositae, and has effects of resisting radiation and aging, preventing and treating arteriosclerosis, and delaying skin aging.

In the invention, ROS is eliminated mainly through the synergistic action of panthenol, uric acid, epigallocatechin gallate, camellia extract and camellia leaf extract; the synthesis of cell antioxidant enzyme is promoted by the synergistic action of curcumin, paeonol and epigallocatechin gallate; the synergistic effect of paeonol, caffeine, dipotassium glycyrrhizinate, epigallocatechin gallate, nicotinamide and silymarin is used for improving autophagy of cells and eliminating cell injury.

Compared with the prior art, the invention has the following beneficial effects:

1. the basis of the antioxidation and the repair of the oxidative damage is the harm of active oxygen, the in-vivo clearing process and the repair process of the oxidative damage, not only the active oxygen is cleared, but also the cells are further promoted to improve the self antioxidation capability, and the oxidation resistance and the repair oxidative damage capability of the cells are improved.

2. The formula of the invention contains an antioxidant component for eliminating active oxygen, and is matched with a synergistic component, so that the autophagy component of cells is increased, the damage of the active oxygen to the skin is reduced, the self antioxidant capacity of the skin is improved, and the capacity of repairing oxidative damage of the skin is accelerated.

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.

Examples 1 to 6

Examples 1-6 provide an antioxidant and repair oxidative damage composition comprising the components in the mass percent amounts shown in table 1.

The compositions of examples 1-6 were prepared as follows:

(1) Weighing the raw materials according to the components and mass percentages of the composition for resisting oxidation and repairing oxidative damage in the table 1;

(2) Mixing flos Camelliae Japonicae extract, folium Camelliae Japonicae extract, water and 1,3-propylene glycol to form mixed solution A;

(3) Adding other raw materials into the mixed solution A, and uniformly stirring.

TABLE 1

Comparative examples 1 to 7

Comparative examples 1 to 7 provide a composition for oxidation resistance and repair of oxidative damage, comprising the components in mass% amounts shown in table 2. The compositions of comparative examples 1 to 7 were prepared in the same manner as in the examples.

TABLE 2

Effect verification:

1) And (3) safety testing: skin irritation

Prepared by using all the examples and comparative examples described aboveThe composition for resisting oxidation and repairing oxidative damage is subjected to a human body safety patch test, and the test method refers to a human body skin patch test in 2015 cosmetic safety technical Specification. The method for the skin closed patch test comprises the following steps: selecting 30 persons with age of 18-60 years, selecting the persons with area not more than 50mm ² And a qualified plaque test device with a depth of about 1mm, 0.020mL of the oxidation and oxidation damage repairing composition prepared in the above examples and comparative examples is put into a small chamber of the plaque test device, a control hole is a blank control (no substance is put), the plaque test device added with the oxidation and oxidation damage repairing composition is applied to the curved side of the forearm of a subject by using a hypoallergenic adhesive tape, and the patch test device is evenly applied to the skin by lightly pressing the palm for 24 hours. Skin reactions were observed at 30min (after disappearance of the indentation), 24h and 48h after removal of the plaque tester containing the composition for oxidation resistance and repair of oxidative damage, respectively, according to the criteria of table 3, and the observation results were recorded (see table 3 for grading criteria). The patch test results for the composition against oxidative damage and repair are shown in table 4.

The experimental results show that the compositions for resisting oxidation and repairing oxidative damage prepared in all the examples and comparative examples pass the safety test of the patch.

TABLE 3 skin response grading Standard for skin Enclosed Patch test

TABLE 4 human body safety test results

Sample numbering	30min	24h	48h	Total result
					Example 1	0	0	0	No positive reaction for 30 people
Example 2	0	0	0	No positive reaction for 30 people
					Example 3	0	0	0	No positive reaction for 30 people
Example 4	0	0	0	No positive reaction for 30 people
					Example 5	0	0	0	No positive reaction for 30 people
Example 6	0	0	0	No positive reaction for 30 people
					Comparative example 1	0	0	0	No positive reaction for 30 people
Comparative example 2	0	0	0	No positive reaction for 30 people
					Comparative example 3	0	0	0	No positive reaction for 30 people
Comparative example 4	0	0	0	No positive reaction for 30 people
					Comparative example 5	0	0	0	No positive reaction for 30 people
Comparative example 6	0	0	0	No positive reaction for 30 people
					Comparative example 7	0	0	0	No positive reaction for 30 people

2) ROS scavenging ability test-Total antioxidant Activity assay (2, 2' -azino-bis (3-ethyllbenzhiazoline-6-sulfonic acid), ABTS method)

The composition for resisting oxidation and repairing oxidative damage prepared in all the examples and the comparative examples is tested for ROS scavenging capacity, a test instrument is an enzyme-labeling instrument, and the test method is as follows: ABTS can be K ₂ S ₂ O ₈ Oxidizing to generate blue-green radical cation ABTS ^.+ ,ABTS ^.+ Quite stable, maximum absorption peaks at 414,645,734 and 805nm, ABTS in the presence of antioxidant ^.+ Reacting with it, it becomes colorless ABTS. ABTS was tested on each composition dilution sample ^.+ The cleaning ability of (1) was tested in parallel for 3 times, the average was taken and ABTS was recorded ^.+ The cleaning ability of (1).

ABTS ^.+ The formula for the clearance of (c) is as follows:

ABTS ^.+ cleaning ability of = (OD 734 nm) _{Control group} -OD734nm _{Sample set} )/OD734nm _{Control group} *100％

Control group-group without sample addition.

Sample set-sample added set.

Test results are shown in Table 5, ABTS ^.+ The stronger the scavenging ability of (a), the stronger the ROS scavenging ability. The composition of the invention has higher ROS scavenging capability.

TABLE 5

Numbering	ABTS. ⁺ Cleaning capability of
		Example 1	92.7％
Example 2	42.7％
		Example 3	87.3％
Example 4	79.1％
		Example 5	72.5％
Example 6	62.7％
		Comparative example 1	34.6％
Comparative example 2	33.2％
		Comparative example 3	35.8％
Comparative example 4	38.0％
		Comparative example 5	36.8％
Comparative example 6	39.8％
		Comparative example 7	36.7％

3) Glutathione peroxidase (GSH-Px) activity test for improving cell self-antioxidant capacity by promoting activity of antioxidant enzyme

The compositions for oxidation resistance and repair of oxidative damage prepared in all of the above examples and comparative examples were subjected to a test for increasing glutathione peroxidase (GSH-Px) activity using a microplate reader. The test method comprises the following steps: the diluted samples of the antioxidant and repair oxidative damage compositions prepared in all of the above examples and comparative examples were added to human keratinocyte HaCat cells, and after 24 hours of incubation, cell lysates were prepared. The preparation of cell lysate is firstly carried out protein quantification, and then the glutathione peroxidase activity of the lysate is tested by using a kit, wherein the unit is U/mg/protein.

The results are shown in Table 6, cellular glutathione peroxidase (GSH-Px) activity. The higher the activity of glutathione peroxidase (GSH-Px), the stronger the antioxidant capacity of the cell itself. The composition of the invention can improve the self antioxidant capacity by improving the activity of cellular antioxidant enzyme.

TABLE 6

4) Increasing autophagy viability of cells

Autophagy is the key to clearing damaged proteins or organelles, and the LC3II level is in direct proportion to the number of autophagic vacuoles, so that the autophagy activity of cells is reflected.

The compositions for oxidation resistance and repair of oxidative damage prepared in all of the above examples and comparative examples were tested for enhancing autophagy activity, and LC3II was tested for autophagy activity by western blotting. The test method comprises the following steps: the diluted samples of the composition for oxidation resistance and repair of oxidative damage prepared in all of the above examples and comparative examples were added to human skin fibroblasts, incubated for 5 hours, and then subjected to UVB irradiation and further incubated for 24 hours to prepare cell lysates. And (3) preparing cell lysate, quantifying protein, and detecting the relative expression quantity of LC3II by using a western blot method.

The calculation formula for improving autophagy activity of the test sample is as follows:

cell autophagy activity improvement rate = (relative expression amount of LC3 II) _{Sample set} Relative expression of LC3II _{Control group} ) Relative amount of expression of/LC 3II _{Control group} *100％

Control group-group without sample addition.

Sample set-sample added set.

The test results are shown in Table 7, and the higher the increase rate of autophagy activity indicates that the sample can increase autophagy activity. The composition has a good effect of improving autophagy activity of cells.

TABLE 7

Numbering	Cell autophagy activity improvement rate
		Example 1	74.0％
Example 2	61.1％
		Example 3	65.1％
Example 4	47.2％
		Example 5	55.2％
Example 6	27.1％
		Comparative example 1	58.8％
Comparative example 2	58.5％
		Comparative example 3	52.0％
Comparative example 4	54.5％
		Comparative example 5	39.9％
Comparative example 6	47.4％
		Comparative example 7	46.7％

The invention has many applications, and the above description is only a preferred embodiment of the invention. It should be noted that the above examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that various modifications can be made without departing from the principles of the invention and these modifications are to be considered within the scope of the invention.

Claims

1. The composition for resisting oxidation and repairing oxidative damage is characterized by comprising the following components in percentage by mass: