CN112704234A - Nutritional composition containing composite peptide and probiotics and preparation method thereof - Google Patents

Nutritional composition containing composite peptide and probiotics and preparation method thereof Download PDF

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Publication number
CN112704234A
CN112704234A CN202011473752.1A CN202011473752A CN112704234A CN 112704234 A CN112704234 A CN 112704234A CN 202011473752 A CN202011473752 A CN 202011473752A CN 112704234 A CN112704234 A CN 112704234A
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parts
peptide
probiotics
nutritional composition
vitamin
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王中振
谢涛
谢骞
刘家生
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Guangzhou Jinguishou Pharmaceutical Co ltd
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Guangzhou Jinguishou Pharmaceutical Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention belongs to the technical field of food processing, and particularly relates to a nutritional composition containing composite peptide and probiotics and a preparation method thereof. The nutritional composition containing the compound peptide and the probiotics provided by the invention comprises the compound peptide, the probiotics, fruit juice, hawthorn powder, prebiotics and vitamin B5Vitamin B6Maltitol, 13 parts of thickening agent, resveratrol and water. The peptide in the nutritional composition containing the composite peptide and the probiotics provided by the invention can be completely absorbed, the survival rate of the probiotics is high, the bacterial balance in intestinal tract can be adjusted, and the intestinal tract is improvedSub-health state, and enhancing immunity. Meanwhile, the nutritional composition containing the compound peptide and the probiotics provided by the invention can supplement vitamin B5Vitamin B6Essential mineral substances and trace elements of human bodies such as vitamin C and iron elements, rich fruit flavor, fragrant and sweet taste and good acceptability.

Description

Nutritional composition containing composite peptide and probiotics and preparation method thereof
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a nutritional composition containing composite peptide and probiotics and a preparation method thereof.
Background
Peptides are formed by the condensation of the amino group of one amino acid and the carboxyl group of another amino acid, are called peptide bonds in protein chemistry, are amphiphilic compounds, and are biologically active substances involved in various cellular functions in the organism. Macroscopically, peptides play a key role in the processes of growth and development, newcastle disease, aging and death of human beings, and if the human bodies lack the peptides, the skills of the human bodies are changed greatly, and the growth and development of the children become slow or even stop. Microscopically, the peptide can regulate the balance of water and electrolyte in vivo, produce antibodies for resisting bacteria and infection for an immune system, improve immunity, promote wound healing, repair cells, improve cell metabolism and prevent cell degeneration.
Probiotics is a kind of active microorganisms beneficial to a host, and is a general term for active beneficial microorganisms which are planted in the intestinal tract and the reproductive system of a human body and can generate definite health efficacy so as to improve the micro-ecological balance of the host and play beneficial roles. The probiotics have the functions of preventing constipation, improving intestinal functions, helping digestion and absorption of nutrient substances and producing a plurality of important nutrient substances including pantothenic acid, nicotinic acid, vitamin B1, vitamin B2, vitamin B6, vitamin K and the like.
In children and the elderly, due to the development of imperfect physiological structures and functions and the deterioration of functions, the children and the elderly are more susceptible to adverse effects caused by environmental pathogens and antibiotics, and the resistance to certain diseases needs to be improved by means of external immune preparations. Therefore, it is important to develop a micro-ecological food which is convenient to drink, rich in nutrition and capable of enhancing immunity.
Patent publication No. CN105641209A discloses a probiotic composition for improving immunity, which comprises probiotic dry powder, radix astragali extract powder, fructus Lycii extract powder, and vitamin K and vitamin B12Pyridoxine, biotin, folic acid, nicotinic acid and thiamine, and is prepared from traditional Chinese medicines and probioticsThe bacteria are reasonably compatible and act synergistically, so that the immunity is remarkably improved, and the health of the organism is promoted. However, the traditional Chinese medicine components are complex, the components which inactivate probiotics are inevitably present to reduce the curative effect, and the traditional Chinese medicine is bitter in taste and generally unacceptable. Patent publication No. CN109123269A discloses a blueberry selenium peptide liquid and a preparation method thereof, wherein the formula components of the blueberry selenium peptide liquid comprise blueberry juice or blueberry fermentation product, protein peptide, maltitol, inulin, sucralose, organic selenium and the like, the peptide liquid can supplement peptide and selenium to a certain extent, but the formula components of the blueberry selenium peptide liquid do not contain substances for promoting absorption, and the peptide is not completely absorbed, so that the beneficial effect is not obvious.
In summary, the prior art generally has the technical defects of probiotic inactivation, incomplete peptide absorption, single taste, poor acceptability and the like.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the present invention aims to provide a nutritional composition containing a composite peptide and probiotics and a preparation method thereof. The peptide in the nutritional composition containing the composite peptide and the probiotics provided by the invention can be completely absorbed, the survival rate of the probiotics is high, the fruit flavor is rich, the taste is fragrant and sweet, the acceptability is good, and the immunity of the organism can be improved.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a nutritional composition containing composite peptide and probiotics comprises the following components in parts by weight:
20-30 parts of compound peptide, 10-16 parts of probiotics, 25-33 parts of fruit juice, 5-9 parts of hawthorn powder, 10-20 parts of prebiotics and vitamin B51-5 parts of vitamin B61-5 parts of maltitol, 10-20 parts of thickening agent, 5-8 parts of resveratrol and 40-50 parts of water.
Further, the nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
Furthermore, the composite peptide in the nutritional composition containing the composite peptide and the probiotics consists of marine protein peptide, marine collagen peptide and bone collagen peptide according to the weight ratio of 15-17:5-8: 9-11.
Furthermore, the probiotics in the nutritional composition containing the composite peptide and the probiotics consist of bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 16-19:13-15: 21-23.
Further, the fruit juice in the nutritional composition containing the composite peptide and the probiotics consists of apple juice, cherry juice, pomegranate juice and grapefruit juice according to the mass ratio of 9:11:7: 15.
Furthermore, the prebiotics in the nutritional composition containing the composite peptide and the probiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 13-15:2-7: 8-11.
Furthermore, the thickening agent in the nutritional composition containing the compound peptide and the probiotics consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 8-11:5-7: 3-5.
The invention also provides a preparation method of the nutritional composition containing the composite peptide and the probiotics, which comprises the following steps:
s1, mixing the compound peptide, the fruit juice and the hawthorn powder according to the formula ratio, and stirring for 10min at the rotation speed of 400-600rpm at 25 ℃ to obtain a mixed solution A;
s2, mixing the thickening agent with water according to the formula ratio, heating to 50-55 ℃, stirring for 15min under the condition of 500-600rpm, adding prebiotics and vitamin B5And vitamin B6Continuously stirring for 20min to obtain a mixed solution B;
s3, cooling the mixed solution B prepared in the step S2 to 20 ℃, adding the mixed solution A prepared in the step S1, stirring for 10min under the conditions of 600-800rpm, adding maltitol and resveratrol, and continuously stirring for 10min to prepare a mixed solution C;
s4, performing high-temperature steam sterilization on the mixed solution C prepared in the step S3 at the sterilization temperature of 120-140 ℃ for 0.5h, then cooling to 20 ℃, adding probiotics according to the formula amount, stirring for 15min at the speed of 500-600rpm, and filling to obtain the product.
According to the formula, repeated research shows that the composite peptide consists of marine protein peptide, marine collagen peptide and ossein protein peptide according to a certain proportion, can rapidly pass through the oral cavity and the stomach of a human body, directly enters the small intestine, is absorbed by the small intestine, finally enters the blood circulation system, organs and cell tissues of the human body, and rapidly plays the physiological action and the biological function.
The fruit juice component and the hawthorn powder in the formula can adjust the taste of the nutritional composition, so that the nutritional composition has rich fruit taste, and the fruit juice contains rich dietary fibers, so that the intestinal tract movement can be promoted, harmful substances in the intestinal tract can be eliminated, and the absorption of the compound peptide is facilitated. The hawthorn powder can promote gastric secretion and increase enzyme in the stomach, thereby accelerating the absorption of the compound peptide. Apple polyphenol contained in apple juice also has the functions of resisting bacteria, protecting cardiovascular system and resisting decayed tooth, cherry juice has good iron supplementing effect, can improve anemia of children, and improve physical quality, pomegranate juice contains rich trace elements and amino acids, can achieve the functions of invigorating stomach, refreshing and enhancing appetite, and grapefruit juice can relieve dyspepsia, thereby being beneficial to full absorption of peptide.
Stachyose has obvious value-added effect on beneficial bacteria in intestinal tract, can quickly improve the internal environment of human digestive tract, regulate microecological bacteria balance, and the isomaltooligosaccharide has positive effect on the digestive system, immune system, metabolic circulation system and the like of human body, and has promotion effect on maintaining the functions of decomposition, transformation, absorption and the like of food in the digestive system, and inulin can promote the growth of beneficial bacteria in the intestinal tract, help to improve constipation and promote the absorption of trace elements. According to the invention, through researches, stachyose, isomaltooligosaccharide and inulin are mixed according to a certain proportion and then taken together with probiotics, so that the metabolism and proliferation of the probiotics can be promoted, the survival rate of the probiotics in the nutritional composition is ensured, the balance of bacteria in intestinal tracts is adjusted, and the sub-health state of the intestinal tracts is improved, thereby enhancing the immunity of organisms.
Vitamin B5And vitamin B6Is two water-soluble vitamins B, and has effects in promoting regeneration of body tissue, and producing antibodyPromoting the production of gastric acid in digestive system, maintaining potassium-sodium balance, etc. and can supplement vitamins and strengthen immunity. The thickening agent in the formula consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to a certain proportion, and has no mutual intersection effect or mutual elimination intersection effect, and has good emulsification stability.
Resveratrol is a natural plant antibody, has strong antioxidant capacity, can remove free radicals, and plays a role of a preservative in the formula of the invention. Meanwhile, due to the strong antioxidant capacity, the resveratrol also has the effects of improving immunity, resisting cancer, resisting virus, resisting aging and the like, and has positive synergistic effect by improving the immunity of a human body together with the compound peptide, the vitamin B5 and the vitamin B6.
Compared with the prior art, the nutritional composition containing the composite peptide and the probiotics and the preparation method thereof provided by the invention have the following technical advantages:
(1) the peptide in the nutritional composition containing the composite peptide and the probiotics provided by the invention can be completely absorbed, the survival rate of the probiotics is high, the bacterial balance in the intestinal tract is adjusted, and the sub-health state of the intestinal tract is improved, so that the immunity of the organism is enhanced;
(2) the nutritional composition containing the compound peptide and the probiotics provided by the invention can supplement vitamin B5Vitamin B6Essential mineral substances and trace elements of human bodies such as vitamin C and iron elements, has rich fruit flavor, fragrant and sweet taste and good acceptability, and can increase the immunity of the organism;
(3) the preparation method of the nutritional composition containing the composite peptide and the probiotics provided by the invention is simple and easy to operate, has controllable conditions and lower production cost, and is easy to realize industrial production.
Detailed Description
The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples. Various modifications can be made by those skilled in the art based on the basic idea of the invention, but it is within the scope of the invention as long as it does not depart from the basic idea of the invention.
In the embodiment, the fruit juice in the nutritional composition containing the composite peptide and the probiotics consists of apple juice, cherry juice, pomegranate juice and grapefruit juice according to the mass ratio of 9:11:7: 15.
Example 1A nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 20 parts of compound peptide, 10 parts of probiotics, 25 parts of fruit juice, 5 parts of hawthorn powder, 10 parts of prebiotics and vitamin B51 part of vitamin B61 part, 10 parts of maltitol, 9 parts of thickening agent, 5 parts of resveratrol and 50 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 15:8: 9; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 16:13: 21; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 13:2: 8; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 8:5: 3.
The preparation method of the nutritional composition containing the composite peptide and the probiotics comprises the following steps:
s1, mixing the compound peptide, the fruit juice and the hawthorn powder according to the formula ratio, and stirring for 10min at the rotation speed of 400rpm at 25 ℃ to obtain a mixed solution A;
s2, mixing the thickening agent with water according to the formula amount, heating to 50 ℃, stirring for 15min under the condition of 500rpm, and adding prebiotics and vitamin B5And vitamin B6Continuously stirring for 20min to obtain a mixed solution B;
s3, cooling the mixed solution B prepared in the step S2 to 20 ℃, adding the mixed solution A prepared in the step S1, stirring for 10min at 600rpm, adding maltitol and resveratrol, and continuously stirring for 10min to prepare a mixed solution C;
s4, performing high-temperature steam sterilization on the mixed liquid C prepared in the step S3 at the sterilization temperature of 120 ℃ for 0.5h, cooling to 20 ℃, adding probiotics according to the formula amount, stirring for 15min at 500rpm, and filling to obtain the probiotic bacteria.
Example 2A nutritional composition comprising Complex peptides and Probiotics and method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the composite peptide and the probiotics comprises the following steps:
s1, mixing the compound peptide, the fruit juice and the hawthorn powder according to the formula ratio, and stirring for 10min at the rotation speed of 500rpm at 25 ℃ to obtain a mixed solution A;
s2, mixing the thickening agent with water according to the formula amount, heating to 53 ℃, stirring for 15min at 550rpm, adding prebiotics and vitamin B5And vitamin B6Continuously stirring for 20min to obtain a mixed solution B;
s3, cooling the mixed solution B prepared in the step S2 to 20 ℃, adding the mixed solution A prepared in the step S1, stirring for 10min at 700rpm, adding maltitol and resveratrol, and continuously stirring for 10min to prepare a mixed solution C;
s4, performing high-temperature steam sterilization on the mixed liquid C prepared in the step S3 at the sterilization temperature of 130 ℃ for 0.5h, then cooling to 20 ℃, adding probiotics according to the formula amount, stirring for 15min at 550rpm, and filling to obtain the probiotic bacteria.
Example 3A nutritional composition comprising Complex peptides and Probiotics and method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 30 parts of compound peptide, 16 parts of probiotics, 33 parts of fruit juice, 9 parts of hawthorn powder, 20 parts of prebiotics and vitamin B55 portions of vitamin B65 parts of maltitol, 20 parts of thickening agent, 8 parts of resveratrol and 40 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 17:5: 11; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 19:15: 23; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 15:7: 11; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 11:7: 5.
The preparation method of the nutritional composition containing the composite peptide and the probiotics comprises the following steps:
s1, mixing the compound peptide, the fruit juice and the hawthorn powder according to the formula ratio, and stirring for 10min at the rotation speed of 600rpm at 25 ℃ to obtain a mixed solution A;
s2, mixing the thickening agent with water according to the formula amount, heating to 55 ℃, stirring for 15min under the condition of 600rpm, and adding prebiotics and vitamin B5And vitamin B6Continuously stirring for 20min to obtain a mixed solution B;
s3, cooling the mixed solution B prepared in the step S2 to 20 ℃, adding the mixed solution A prepared in the step S1, stirring for 10min at 800rpm, adding maltitol and resveratrol, and continuously stirring for 10min to prepare a mixed solution C;
s4, performing high-temperature steam sterilization on the mixed liquid C prepared in the step S3 at the sterilization temperature of 140 ℃ for 0.5h, then cooling to 20 ℃, adding probiotics according to the formula amount, stirring for 15min at 600rpm, and filling to obtain the product.
Comparative example 1, a nutritional composition comprising a complex peptide and a probiotic and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics and fruits28 parts of juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 1:1: 1; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: the composite peptide in the comparative example consists of marine protein peptide, marine collagen peptide and bone collagen peptide according to the weight ratio of 1:1: 1.
Comparative example 2, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: the fruit juice in the comparative example consists of apple juice, cherry juice, pomegranate juice and grapefruit juice according to the mass ratio of 1:1:1: 1.
Comparative example 3, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: no hawthorn powder was added in this comparative example.
Comparative example 4, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: resveratrol was not added in this comparative example.
Comparative example 5, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 1:1: 1; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: in the comparative example, the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 1:1: 1.
Comparative example 6, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: no vitamin B was added to this comparative example5
Comparative example 7, a nutritional composition comprising a Complex peptide and Probiotics and a method for preparing the same
The nutritional composition containing the compound peptide and the probiotics comprises the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
The composite peptide consists of marine protein peptide, marine collagen peptide and ossein peptide according to the weight ratio of 16:7: 10; the probiotics comprise bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 17:14: 22; the prebiotics consist of stachyose, isomaltooligosaccharide and inulin according to the mass ratio of 14:5: 9; the thickening agent consists of tamarind polysaccharide gum, sodium caseinate and guar gum according to the mass ratio of 9:6: 4.
The preparation method of the nutritional composition containing the complex peptide and the probiotics is similar to that of example 2.
The comparative example differs from example 2 in that: no vitamin B was added to this comparative example6
Test example one, test for modulating intestinal flora with nutritional composition
Test samples: examples 1 to 3, comparative example 5, the prepared nutritional composition containing the complex peptide and the probiotic bacteria;
the test method comprises the following steps: mice adopting salmonella to construct a diarrhea model are divided into 4 groups, the groups of examples 1-3 and comparative example 5 drink the nutrient composition containing the compound peptide and the probiotics prepared in the examples 1-3 and the comparative example 5 every day, and the blank group drinks elegant purified water every day; after feeding 4 groups of mice for 30 days, observing the diarrhea condition of the mice; one day before the experiment, feces were collected and stored at-80 ℃ for intestinal flora analysis. Carrying OUT 16S rDNA high-throughput gene sequencing on the excrement of each group of mice, wherein the sequencing region is a V3-V4 region, and obtaining the OUT number of each group through sequencing;
and (3) test results: the test results are shown in Table 1.
TABLE 1 nutritional compositions for modulating the effects of intestinal flora
Group of Whether or not diarrhea is present Number of OUT
Example 1 Whether or not 275
Example 2 Whether or not 281
Example 3 Whether or not 273
Comparative example 5 Is that 234
Blank group Is that 215
As can be seen from table 1, the nutritional composition containing the composite peptide and the probiotics provided by the invention has good efficacy of regulating intestinal flora, and can effectively improve diarrhea, activate gastrointestinal functions and promote intestinal health. The mice in the group of the example 2 have the highest fecal OUT number of 281, and compared with the blank group, 66 is increased, and the increment is obvious, so the example 2 is the best example of the invention. Compared with the example 2, the comparative example 5 changes the dosage ratio of the prebiotics in the formula components, but the efficacy of the prepared nutritional composition containing the compound peptide and the probiotics for regulating the intestinal flora is reduced, which shows that the dosage ratio of the prebiotics in the formula of the invention is optimized, the metabolism and proliferation of the probiotics can be promoted under the condition of the dosage ratio, the survival rate of the probiotics in the intestinal tract after the nutritional composition is drunk is ensured, the bacterial balance in the intestinal tract is regulated, and the sub-health state of the intestinal tract is improved.
Test example two, test of immunomodulating Effect of nutritional composition
Test samples: the nutritional compositions containing the complex peptide and the probiotics prepared in examples 1 to 3, comparative examples 1 to 4, and comparative examples 6 to 7;
the test method comprises the following steps: selecting 100 ICR healthy magnetic mice of 6-8 weeks provided by the Experimental animals center of the department of medicine of Beijing university, randomly dividing the ICR healthy magnetic mice into 10 groups, each group comprises 10 ICR healthy magnetic mice, the nutritional compositions containing the compound peptide and the probiotics are prepared by respectively gavage the stomach of the embodiments 1-3, the comparative examples 1-4 and the comparative examples 6-7, the blank groups are gavage with elegant purified water, the stomach is gavage for 1 time every day, each gavage is 0.1mL/10g of the weight of the mice, the animals freely eat and drink water during the experiment period, and the mice are killed to measure various indexes after the mice are gavage for 4 weeks;
determination of proliferation capacity of Con A-induced splenic lymphocytes in mice: aseptically collecting spleen, grinding, filtering (200 mesh), washing, suspending, and making into cell with cell concentration of 5 × 106Spleen cell suspension, adding the spleen cell suspension into 24-well culture plate in 2-well, each well containing 1mL of 1. mu.l Con A in experimental well and no Con A in another well as control well, and adding 5% CO2After culturing at 37 ℃ for 72 hours, the A value was measured at a wavelength of 570nm by the MTT method, and the difference between the A values of the experimental wells and the control wells was used as the valueLymphocyte competence;
and (3) test results: the test results are shown in Table 2.
Table 2 nutritional composition immunomodulating test results
Figure BDA0002836910670000111
As can be seen from table 2, the nutritional composition containing the composite peptide and the probiotics provided by the present invention has a good effect of enhancing immunity, and the proliferation ability of mouse lymphocytes and the swelling degree of foot metatarsal swelling induced by ConA are significantly improved compared with the blank group, wherein the proliferation ability of mouse lymphocytes and the swelling degree of foot metatarsal swelling induced by ConA of the nutritional composition containing the composite peptide and the probiotics prepared in example 2 are the most improved, which is the best example of the present invention.
Compared with example 2, comparative example 1 changed the dosage ratio of the composite peptide, but the effect of enhancing immunity of the prepared nutritional composition was reduced, which indicates that the dosage and ratio of the composite peptide in the formulation of the present invention have reached the optimal values. Comparative examples 2 and 3 change the dosage ratio and dosage of the fruit juice and the hawthorn powder, but the prepared nutritional composition has reduced effect of enhancing immunity, which indicates that the fruit juice and the hawthorn powder can promote the full absorption of the compound peptide, thereby achieving the effect of enhancing the body immunity. Comparative example 4 where resveratrol was not added, but the resulting nutritional composition had a reduced effect on enhancing immunity, indicating that resveratrol, while acting as a preservative, may also act in combination with a complex peptide and vitamin B5Vitamin B6Has synergistic effect and can improve immunity. In comparative example 6 and comparative example 7, no vitamin B was added5Vitamin B6However, the resulting nutritional composition has a reduced effect on enhancing immunity, which indicates that vitamin B is present5Vitamin B6Can supplement vitamins and enhance immunity.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art will recognize that changes may be made to the embodiments described above without departing from the spirit and scope of the invention. Therefore, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the technical spirit of the present invention are covered by the claims of the present invention.

Claims (8)

1. The nutritional composition containing the composite peptide and the probiotics is characterized by comprising the following components in parts by weight:
20-30 parts of compound peptide, 10-16 parts of probiotics, 25-33 parts of fruit juice, 5-9 parts of hawthorn powder, 10-20 parts of prebiotics and vitamin B51-5 parts of vitamin B61-5 parts of maltitol, 10-20 parts of thickening agent, 5-8 parts of resveratrol and 40-50 parts of water.
2. The nutritional composition containing the composite peptide and the probiotics according to claim 1, which is characterized by comprising the following components in parts by weight: 26 parts of compound peptide, 14 parts of probiotics, 28 parts of fruit juice, 7 parts of hawthorn powder, 16 parts of prebiotics and vitamin B53 portions of vitamin B64 parts of maltitol, 15 parts of thickening agent, 6 parts of resveratrol and 48 parts of water.
3. The nutritional composition according to claim 1 or 2, wherein the complex peptide consists of marine protein peptide, marine collagen peptide and bone collagen peptide in a weight ratio of 15-17:5-8: 9-11.
4. The nutritional composition containing the complex peptide and the probiotics according to claim 1 or 2, wherein the probiotics consist of bifidobacterium ovorans, lactobacillus casei and streptococcus thermophilus according to the mass ratio of 16-19:13-15: 21-23.
5. The nutritional composition containing the complex peptides and the probiotics according to claim 1 or 2, wherein the fruit juice consists of apple juice, cherry juice, pomegranate juice and grapefruit juice in a mass ratio of 9:11:7: 15.
6. The nutritional composition containing the complex peptide and the probiotics as claimed in claim 1 or 2, wherein the prebiotics comprise stachyose, isomaltooligosaccharide and inulin at a mass ratio of 13-15:2-7: 8-11.
7. The nutritional composition containing the complex peptide and the probiotics as claimed in claim 1 or 2, wherein the thickener consists of tamarind polysaccharide gum, sodium caseinate and guar gum in a mass ratio of 8-11:5-7: 3-5.
8. A method for preparing a nutritional composition comprising a complex peptide and a probiotic according to any of claims 1 to 7, comprising the steps of:
s1, mixing the compound peptide, the fruit juice and the hawthorn powder according to the formula ratio, and stirring for 10min at the rotation speed of 400-600rpm at 25 ℃ to obtain a mixed solution A;
s2, mixing the thickening agent with water according to the formula ratio, heating to 50-55 ℃, stirring for 15min under the condition of 500-600rpm, adding prebiotics and vitamin B5And vitamin B6Continuously stirring for 20min to obtain a mixed solution B;
s3, cooling the mixed solution B prepared in the step S2 to 20 ℃, adding the mixed solution A prepared in the step S1, stirring for 10min under the conditions of 600-800rpm, adding maltitol and resveratrol, and continuously stirring for 10min to prepare a mixed solution C;
s4, performing high-temperature steam sterilization on the mixed solution C prepared in the step S3 at the sterilization temperature of 120-140 ℃ for 0.5h, then cooling to 20 ℃, adding probiotics according to the formula amount, stirring for 15min at the speed of 500-600rpm, and filling to obtain the product.
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