CN108795823A - It is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application - Google Patents
It is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application Download PDFInfo
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- CN108795823A CN108795823A CN201810722697.1A CN201810722697A CN108795823A CN 108795823 A CN108795823 A CN 108795823A CN 201810722697 A CN201810722697 A CN 201810722697A CN 108795823 A CN108795823 A CN 108795823A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of cultural method improving women pregnant and lying-in women intestinal flora probiotics and applications.Porous material is added in the culture medium containing the somatomedin for promoting growth of probiotics breeding, and the volume ratio of culture medium and porous material is 1：0.001–1：1000, then be inoculated with probiotics in the medium and cultivated, realize probiotics High Density Cultivation；The probiotics obtained can be used for preparing the products such as food, food additives, medicine, feed or environmental protection treatment.The present invention, which obtains high density probiotics, has the performances such as good heat resistance, acid resistance and drug resistance, has broad application prospects in fields such as food, medicine, feed, environmental protection.
Description
Technical field
The present invention relates to a kind of cultural method of probiotics improving women pregnant and lying-in women's intestinal flora and applications, belong to micro- life
Object preparation culture technique field.
Background technology
Probiotics, mono- words of English probiotics are translated into " for life " from Greek " for live " derivation, are
Viable bacteria containing physiological activity after oral or other administering modes take in quantity appropriate, can be colonized in when by body
Host simultaneously improves host's microecological balance, to play beneficial effect.Recent studies indicate that the bacterium in human body is up to
Bacterium in more than 1000 kinds, especially gastrointestinal tract is the abundantest, accounts for whole body micro-ecological bacterial sum 80% or so, is human body cell
It 10 times, is known as " the second genome " of the mankind.These bacteriums contribute to human consumption's food, generate vitamin to prevent food
The disease that middle bacterium is induced, while stimulating immune system.With going deep into for research, find enteric microorganism in many chronic diseases
In disease and symptom, such as inflammation, obesity, diabetes, also cancer also play key effect.The quantity and gene dosage of microorganism
It is surprising, the strong health for influencing the mankind.These are found to be probiotic products in food, health products, medicine, cultivation, environment, health
The application in the fields such as articles for use brings huge motive force.Currently, probiotics is widely used in Yoghourt, sour milk beverage, prebiotic
In the products such as bacteria powder, animal feed, environmental improvement, relatively complete and ripe probiotics industry is formd.
It is worth noting that, when probiotics only reaches certain quantity levels, probiotics could generate in host
Corresponding effect.So needing to ensure contained prebiotic bacterium number living using probiotics as the food of functional component, health products or drug
Amount is maintained at certain level.This demand proposes corresponding requirement to the production and preparation of probiotics.In the training of probiotics
During supporting, realizes High Density Cultivation, can not only meet the functional requirement of follow-up probiotic products, probiotics can also be improved
Production efficiency in production link reduces volume of culture, shortens the production cycle, reduces production cost.Therefore, probiotics is highly dense
Degree culture is the key technical problem in current probiotics exploitation and production.
In liquid fermentation system, Chinese invention patent (publication number：CN 101230323A) alkalinity is added in incubation
Suffered stress material acid or oxygen, make finally to produce when salt and deoxidier are to eliminate lactobacillus acidophilus or bifidobacterium bifidum fermentation
Number of viable improves 2-4 times in product.Chinese invention patent (publication number：CN 101418270A) in the training of Lactobacillus casei Zhang
During supporting, the method by the way that the neutralizers such as NaOH, KOH or ammonium hydroxide and logical nitrogen are added obtains 1011CFU/mL or more viable bacterias
The freeze-drying bacterium powder of several Lactobacillus caseis.Hayakawa K et al. (J Ferment Bioeng, 1990,70,404) use film skill
Art is coupled with fermentation tank, is constantly filtered to remove the stress ingredient such as lactic acid, is realized that highest cell density is up to 1011CFU/mL, the party
Method need to expend mass propgation base, and be only limitted to laboratory level, it is difficult to be expanded to production application scale.
In semisolid or solid fermentation system, Chinese invention patent (publication number：CN 105132321A) using by wheat bran and
The culture medium of the material compositions such as dregs of beans, inoculation enterococcus faecium carry out solid state fermentation, and the viable bacteria content in dry products is up to 3.8
×1010CFU/g。
Similar also has Chinese patent (publication number：CN 101555510B；CN 101555511B；CN 103421710B；
CN 102907563B；CN 103289937B；CN 1212391C) it is all made of dregs of beans, wheat bran, corncob, rice bran, peanut shell, shallow lake
The agricultural wastes such as powder, corn flour, turf or agricultural product solid medium as main component, microbe inoculation carry out highly dense
Degree ferments, and the viable count in product is essentially 1011CFU/g.This kind of product is mainly used for animal feed or carries out the metabolism such as drug
Product produces.
Probiotics is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can generate
Definite health efficacy so as to improve host's microecological balance, play the active beneficial microorganism general name of beneficial effect.Human body moves
Beneficial bacterium or fungi mainly have in object：Clostridium butyricum, lactobacillus, Bifidobacterium, actinomyces, saccharomycete etc..Generation at present
The most powerful product of the function of being studied in boundary be mainly the above all kinds of microorganism groups at composite reactive probiotics, extensive use
In bioengineering, industrial or agricultural, food security and life and health field.
The benefit of probiotics is very more, such as prevents or improves diarrhea, alleviate lactase deficiency shape, prevent system genitale togetherness
It contaminates, enhance human immunity, enteron aisle digestive system health, reduction serum cholesterol and help is promoted to absorb nutritional ingredient.
Common probiotics such as Bifidobacterium is existing a kind of physiological bacterium in human body, it is close not with the health of human body
It can divide, if Bifidobacterium will generate lactic acid and acetic acid after sugar decomposition, make enteron aisle in acidity, result can be controlled to be caused by harmful bacteria
Abnormal fermentation, and stimulate enterocinesia, play the role of releasing constipation, bifidobacterium preparations can also inhibit toxigenic
Harmful bacteria quantity, to play good therapeutic effect to liver patient, in addition it is solid can to influence courage for the beneficial bacteriums such as Bifidobacterium
The metabolism of alcohol is translated into the non-absorbent steroids of human body, reduces the concentration of Blood Cholesterol, thus to hypertension and moves
Arteries and veins hardening has certain preventive and therapeutic effect.
Also common lactobacillus is a section for bacterium, is Gram-positive, sporeless bacterium, lactic acid bacteria is in nature
Be distributed in boundary it is very wide, plant surface, dairy products, meat products, beer, grape wine, fruit juice, brewer's wort, fermented dough, sewage with
And in human and animal excreta, separate.The food of Chinese tradition, such as pickles, hot pickled mustard tuber, pickle and the making of wine brewing preservation technology
This effect of lactic acid bacteria is exactly utilized, to enteron aisle and good absorbing.
The many health problems of human body are caused by internal flora is unbalance, and determinant is the balance of Tiny ecosystem, can be passed through
Internal flora is set to achieve the purpose that the ecological balance again to realize alleviation and treatment.For example diarrhea, constipation, vagina infection etc. are comprehensive
Symptom has already passed through years of researches and practice both at home and abroad, it was demonstrated that can be effectively using the probiotics specifically confirmed through clinic
It is treated.
These are found to be application of the probiotic products in fields such as food, health products, medicine, cultivation, environment, amenities
Bring huge motive force.Currently, probiotics be widely used in Yoghourt, sour milk beverage, probiotics powder agent, animal feed,
In the products such as environmental improvement, relatively complete and ripe probiotics industry is formd.
If Chinese patent 201110442521.9 discloses a kind of food product, it is related to being preferentially used for the people's at paediatrics age
The administration form of load capacity height and the probiotic bacteria of stable load, the invention further relate to chocolate flavoured Probiotic supplement,
Especially include the probiotic tablet of interior section and exterior section, but the taste of the invention is chocolate flavoured, limits consumption
Person crowd, although the food of the invention is liked by children deeply, most of adult does not like the food of dietetic chocolate taste
Product, and the food is also not suitable for large amounts of food.
In addition, pregnancy and lactation are two special body periods of mother, if this stage is sick, it is easy to be absorbed in nothing
Available awkward situation is wanted, at this time probiotic supplemented, on the one hand can improve the resistance of mother, on the other hand can also improve mother's
Healthy malaise symptoms.In addition many pregnant and lying-in women can be easy to happen constipation, and probiotics can play good work during this time
With because probiotics plays the role of prevention and relief of constipation, probiotics refers to by adjusting intestinal flora, it is strong improving enteron aisle
The viable bacteria of health, probiotics can adjust intestinal flora balance, maintain the active microorganism of intestinal health.Probiotics can not only prevent
Allergic constitution, but also the immunity of pregnant and lying-in women and baby can be improved, pregnant and lying-in women take probiotics and are conducive to health in right amount.
As Chinese patent 201110442521.9 discloses a kind of compound probiotic, its answering in treating anaphylactia
With and allergy free probiotic electuary for pregnant, the compound probiotic of the invention its by bifidobacterium lactis Bi-07, Lactobacillus rhamnosus
LR22, lactobacillus acidophilus NCFM compositions, can not only stimulate the secretion of IFN-γ and IL-2 in animal body, and can inhibit immune
The secretion of globulin E (IgE) and IL-4, to play prevention and treatment anaphylactia, and the test of pesticide effectiveness shows the drug system
Agent has remarkable effect to improving IL-2 and IFN-γ.In addition, with control group ratio, the IgE contents of test group are significantly reduced,
But the invention do not provide how the method for the culture of the high density and activity compound probiotic, therefore it is compound in the invention
Probiotics is likely to over time, and the quantity of beneficial bacterium is reduced rapidly, to the value that loses one's health.
Therefore it provides a kind of probiotic composition that can improve pregnant and lying-in women's intestinal flora has prodigious realistic meaning.
So need at present it is a kind of improve women pregnant and lying-in women intestinal flora probiotics cultural method and application；Need a kind of improvement female
The property multifarious probiotic composition of pregnant and lying-in women's intestinal flora.
Invention content
In order to solve the problems, such as background technology, the purpose of the present invention is to provide a kind of improvement women pregnant and lying-in women's intestines
The cultural method of road flora probiotics and application, it is characterised in that include the following steps：
The technical solution adopted by the present invention is as follows：
Add porous material in the culture medium containing the somatomedin for promoting growth of probiotics breeding, culture medium with it is more
The volume ratio of Porous materials is 1：0.001–1：1000, then be inoculated with probiotics in the medium and cultivated, realize that probiotics is high
Density culture.
The culture is Anaerobic culturel, and the probiotics being inoculated with is the microorganism of anaerobism or amphimicrobian.
Anaerobic culturel is to cultivate 12-48h at 30-42 DEG C.
After culture, the probiotics gathers the surface of porous material either inside or surface and inside, obtains
Total viable count is up to 1011-1015CFU/g or more in product after drying.Viable count is influenced by factors such as drying means, cold
Viable count higher in dry product is lyophilized.
Preparation-obtained probiotics has the performances such as good heat resistance, acid resistance and drug resistance.
The culture medium is liquid, semisolid or solid-state, and cultivating system can be Liquid Culture, Semi-solid cell culture and consolidate
Any one of three kinds of training methods of body culture, the porous material are solid-state.
The invention discloses a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, the probiotic groups
It closes object and contains that resistant dextrin, inulin, oligofructose, bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus casei etc. are more, this is prebiotic
Bacteria composition can improve women pregnant and lying-in women's intestinal flora, but also can by adding maltose, and adjust oligofructose and
The weight ratio and resistant dextrin of inulin and the weight ratio of Lactobacillus rhamnosus, significantly improve probiotics in probiotic composition
Survival rate, extend the pot-life of probiotic composition.The present invention relates to a kind of multifarious composition of improvement intestinal flora,
In particular it relates to a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora.
Probiotics there are many containing in the probiotic composition, makes various probiotics pass through interaction and effectively improves women
Pregnant and lying-in women's intestinal flora, and the probiotic composition prepared by the present invention has the characteristics that high activity, by prolonged normal
It is gentle to put, still maintain higher probiotic active.
Explanation：All numbers each mean parts by weight in the present invention.
A kind of probiotic composition includes by weight：
40-60 parts of resistant dextrin, 5-15 parts of inulin, 5-15 parts of oligofructose, 2-8 parts of bifidobacterium lactis, rhamnose breast bar
2-10 parts of bacterium, 2-8 parts of Lactobacillus casei, 2-8 parts of lactobacillus acidophilus, 2-10 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri,
2-10 parts of 2-10 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
Preferably, the probiotic composition includes by weight：It is 40-55 parts of resistant dextrin, 5-12 parts of inulin, oligomeric
5-12 parts of fructose, 2-6 parts of bifidobacterium lactis, 2-8 parts of Lactobacillus rhamnosus, 2-6 parts of Lactobacillus casei, 2-6 parts of lactobacillus acidophilus,
2-8 parts of 2-8 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, 2-8 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
It is further preferred that the probiotic composition includes by weight：45-55 parts of resistant dextrin, inulin 8-12
Part, 8-12 parts of oligofructose, 4-6 parts of bifidobacterium lactis, 5-8 parts of Lactobacillus rhamnosus, 4-6 parts of Lactobacillus casei, acidophilus breast bar
5-8 parts of 4-6 parts of bacterium, 5-8 parts of Lactobacillus helveticus, 5-8 parts of lactobacillus reuteri, 5-8 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
In the probiotic composition, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition, the weight ratio of oligofructose and inulin addition is 1-3:1；Preferably, oligofructose
Weight ratio with inulin addition is 1-1.5:1；It is highly preferred that oligofructose and the weight ratio of inulin addition are 1:1.
In the probiotic composition, the weight ratio of resistant dextrin and Lactobacillus rhamnosus addition is 4-30:1；Preferably,
Resistant dextrin and the weight ratio of Lactobacillus rhamnosus addition are 8-20:1；It is highly preferred that resistant dextrin and Lactobacillus rhamnosus add
The weight ratio added is 3:1.
In some preferred embodiments, 0.5-3 parts of maltose is also contained in the probiotic composition；Some more
In preferred embodiment, 0.5-2.5 parts of maltose is also contained in the probiotic composition；
In some preferred embodiments, oligofructose and the weight ratio of inulin are 1 in the probiotic composition:1.
In some preferred embodiments, the weight ratio of resistant dextrin and Lactobacillus rhamnosus in the probiotic composition
For 8-10:1；
Compared with prior art, beneficial effects of the present invention are：
(1) probiotic composition of the present invention, wherein can interact between each bacterial strain, to advantageously promote women pregnant
Absorption of puerpera's stomach and intestine to nutriment can increase beneficial to bacterial content, play promotion digestion, stimulate intestine immunity cell, increase
The effects that strong women pregnant and lying-in women's intestinal immunity.
(2) present invention adjusts weight ratio and the oligomeric fruit of resistant dextrin and Lactobacillus rhamnosus by adding maltose
The weight ratio of sugar and inulin, hence it is evident that improve stability of probiotics during preservation in composition.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
The probiotic composition of the embodiment includes by weight：40 parts of resistant dextrin, 5 parts of inulin, 10 parts of oligofructose,
2 parts of bifidobacterium lactis, 2 parts of Lactobacillus rhamnosus, 2 parts of Lactobacillus casei, 8 parts of lactobacillus acidophilus, 10 parts of Lactobacillus helveticus, Roy
10 parts of 8 parts of family name's lactobacillus, 10 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 2:1；
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 9:1.
Embodiment 2
Probiotic composition includes by weight in the embodiment：50 parts of resistant dextrin, 10 parts of inulin, oligofructose 10
Part, 5 parts of bifidobacterium lactis, 6 parts of Lactobacillus rhamnosus, 4 parts of Lactobacillus casei, 6 parts of lactobacillus acidophilus, 6 parts of Lactobacillus helveticus, sieve
6 parts of 6 parts of Yi Shi lactobacillus, 6 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1；
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 8.3:1.
Embodiment 3
Probiotic composition includes by weight in the embodiment：60 parts of resistant dextrin, 10 parts of inulin, oligofructose 15
Part, 8 parts of bifidobacterium lactis, 10 parts of Lactobacillus rhamnosus, 8 parts of Lactobacillus casei, 2 parts of lactobacillus acidophilus, 2 parts of Lactobacillus helveticus,
2 parts of 2 parts of lactobacillus reuteri, 2 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1.5:1；
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 6:1；
Embodiment 4
The composition and embodiment 1 of the probiotic composition of the embodiment are identical, only difference is that:The embodiment is in reality
On the basis of the probiotic composition for applying example 1,1.5 parts of maltose is increased.
Comparative example 1
Probiotic composition includes by weight in the embodiment：35 parts of resistant dextrin, 4 parts of inulin, 4 parts of oligofructose,
1 part of bifidobacterium lactis, 1 part of Lactobacillus rhamnosus, 1 part of cheese breast bar, 10 parts of lactobacillus acidophilus, 12 parts of Lactobacillus helveticus, Roy
12 parts of 10 parts of family name's lactobacillus, 12 parts of Lactobacillus rhamnosus and lactobacillus fermenti；
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1；
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 35:1.
Comparative example 2
Probiotic composition includes by weight in the embodiment：66 parts of resistant dextrin, 16 parts of inulin, oligofructose 16
Part, 10 parts of bifidobacterium lactis, 12 parts of Lactobacillus rhamnosus, 10 parts of cheese breast bar, 1 part of lactobacillus acidophilus, 1 part of Lactobacillus helveticus,
1 part of lactobacillus reuteri, 1 part of Lactobacillus rhamnosus, 1 part of lactobacillus fermenti；
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder；
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1；
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 5.5:1.
The detection of 1 flora abundance of experimental example
The detection of flora abundance is carried out to the probiotic composition in embodiment 1-4 and comparative example 1-2：The survey of flora abundance
Determine method：Take growth conditions good and each 35 of female rats in pregnancy period and term, and weight is 250-350g, by 70
Female mice is randomly divided into 7 groups, every group of 5 pregnancy period female mices and 5 term female mices, totally 10；Wherein 4 groups are experimental group, and 3 groups are pair
It is unified to feed 2 weeks under identical environment according to group, continuous 15 days gavages then are carried out to female mice, are 20g/kg according to dosage
Gavage is carried out, experimental group uses the probiotic composition in 1-4 of the embodiment of the present invention to carry out gavage, and control group uses comparative example 1-
Probiotic composition and physiological saline in 2 carry out gavage as blank control, after 15 days gavages, collect the excrement of female mice
Just sample, the 16S rRNA genes for full flora are investigated and analysed, and the abundance of flora in female mice body is detected, and ask average in group
Value, testing result are as shown in table 1；
Explanation：(1) present invention in lactobacillus acidophilus survival rate, refer to that solid beverage is placed 4 under conditions of 25 DEG C
The survival rate of lactobacillus acidophilus after a month；(2) "-" in table of the present invention indicates, this is not present.
Embodiment 1-4 and comparative example 1-2 and blank control are compared, it is found that the present invention passes through in composition
The middle a variety of probiotics of addition can promote the abundance of flora in rat body to 450 or more by 400.
Table 1
The 2 probiotics pot-life of experimental example tests
Probiotic composition in embodiment 1-3 and comparative example 1-2 is subjected to pot-life test：37 DEG C of environment preserves
192 hours, and 0 hour, 6 hours, 12 hours, 18 hours, 24 hours, 96 hours 48 hours, 144 hours and 192 small time-divisions
Other to carry out survival volume test to probiotics and average, the results are shown in Table 2；
It can be found that the probiotics survival rate being added in the embodiment 4 of 1.5 portions of maltose is preserved by 192 hours
In the process, obviously higher than other embodiment and comparative example；In addition, by embodiment 2 with except the other embodiment of embodiment 4 and right
It being found when ratio is compared, the probiotics survival rate of embodiment 2 is above other embodiment and comparative example except embodiment 4,
That is, applicant is found surprisingly that after adding 1.5 parts of maltose in probiotic composition, probiotic composition
Pot-life it is obviously elongated；In addition, when oligofructose in probiotic composition and the weight ratio of inulin are 1:1, and resist simultaneously
Property dextrin and Lactobacillus rhamnosus weight ratio be 8-10:When 1, the pot-life of probiotic composition is significantly longer.
Table 2
Claims (10)
1. a kind of cultural method improving women pregnant and lying-in women intestinal flora probiotics and application, it is characterised in that：Containing promotion
Porous material is added in the culture medium of the somatomedin of growth of probiotics breeding, and the volume ratio of culture medium and porous material is 1：
0.001–1：1000, then be inoculated with probiotics in the medium and cultivated, realize probiotics High Density Cultivation.
2. a kind of cultural method improving women pregnant and lying-in women intestinal flora probiotics according to claim 1 and application,
It is characterized in that：The culture is Anaerobic culturel, and the probiotics being inoculated with is the microorganism of anaerobism or amphimicrobian.
3. a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, which is characterized in that including：Resistance is pasted
Essence, inulin, oligofructose, bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus, Lactobacillus helveticus, sieve
Yi Shi lactobacillus, Lactobacillus rhamnosus and lactobacillus fermenti.
4. a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, which is characterized in that include by weight：
40-60 parts of resistant dextrin, 5-15 parts of inulin, 5-15 parts of oligofructose, 2-8 parts of bifidobacterium lactis, 2-10 parts of Lactobacillus rhamnosus,
2-8 parts of Lactobacillus casei, 2-8 parts of lactobacillus acidophilus, 2-10 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, rhamnose breast bar
2-10 parts of 2-10 parts of bacterium and lactobacillus fermenti.
5. a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, which is characterized in that include by weight：
40-55 parts of resistant dextrin, 5-12 parts of inulin, 5-12 parts of oligofructose, 2-6 parts of bifidobacterium lactis, 2-8 parts of Lactobacillus rhamnosus,
2-6 parts of Lactobacillus casei, 2-6 parts of lactobacillus acidophilus, 2-8 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, rhamnose breast bar
2-8 parts of 2-8 parts of bacterium and lactobacillus fermenti.
6. a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, which is characterized in that include by weight：
45-55 parts of resistant dextrin, 8-12 parts of inulin, 8-12 parts of oligofructose, 4-6 parts of bifidobacterium lactis, 5-8 parts of Lactobacillus rhamnosus,
4-6 parts of Lactobacillus casei, 4-6 parts of lactobacillus acidophilus, 5-8 parts of Lactobacillus helveticus, 5-8 parts of lactobacillus reuteri, rhamnose breast bar
5-8 parts of 5-8 parts of bacterium and lactobacillus fermenti.
7. in the probiotic composition as described in claim 3-6, wherein oligofructose and the weight ratio of inulin addition is 1-3:
1；Preferably, oligofructose and the weight ratio of inulin addition are 1-1.5:1；It is highly preferred that the weight of oligofructose and inulin addition
Amount is than being 1:1.
8. in the probiotic composition as described in claim 3-7, the wherein weight of resistant dextrin and Lactobacillus rhamnosus addition
Than for 4-30:1；Preferably, resistant dextrin and the weight ratio of Lactobacillus rhamnosus addition are 8-20:1；It is highly preferred that resistance is pasted
Essence and the weight ratio of Lactobacillus rhamnosus addition are 3:1.
9. in the probiotic composition as described in claim 3-8, also containing 0.5-3 parts of maltose.
10. in the probiotic composition as described in claim 3-9, the weight ratio of wherein resistant dextrin and Lactobacillus rhamnosus is
8-10:1。
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