CN112656870B - Anti-fatigue traditional Chinese medicine composition and preparation method and application thereof - Google Patents
Anti-fatigue traditional Chinese medicine composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an anti-fatigue traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 3-5 parts of wolfberry fruit, 1-2 parts of ginseng, 3-5 parts of epimedium and 3-5 parts of glossy privet fruit. The composition has remarkable anti-fatigue effect and is suitable for being taken by people who are easy to fatigue for a long time.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, and in particular relates to an anti-fatigue traditional Chinese medicine composition and a preparation method and application thereof.
Background
According to the investigation of the world health organization, more than 35% of people worldwide are in fatigue state, especially 60% -75% of middle-aged male people in fatigue state. Medical experts point out that sustained over-fatigue can lead to a series of adverse symptoms, resulting in "fatigue syndrome". Fatigue is one of the main factors endangering human health in the 21 st century. In the modern society, due to the accelerated pace of life, the working pressure, the family burden and the natural aging of human organs, more and more people feel that the physical conditions are poor, many people bear great psychological pressure for a long time or are in overtime overload working states, such as unreasonable rest and adjustment, and in the past, the functions of important organs of the body are disordered, so that a series of fatigue symptoms such as lassitude, dizziness, headache, limb weakness, poor spirit, hypomnesis and the like are caused. Fatigue is the source of all diseases, and can cause oxygen free radical accumulation, low immune function and the like, and diseases can follow over time, thereby seriously affecting the work and life of people.
At present, products for relieving physical fatigue in the market mainly comprise western medicines and traditional Chinese medicines. Western medicine products are mainly used for treating symptoms to relieve discomfort, but are often used for damaging internal organs (mainly liver and kidney) of a body or forming medicine dependence, so that the western medicine is not suitable for long-term use. The center of gravity of modern medicine has turned towards effective prevention of intervention and disease in pre-disease states. The traditional Chinese medicine has unique characteristics in the aspect of adjusting the whole functional state of the body, and the health food which is prepared by taking the traditional Chinese medicine as the raw material and has the function of relieving physical fatigue has irreplaceable advantages. Traditional Chinese medicine emphasizes the holistic concept and emphasizes holistic treatment on treatment, which has a comprehensive regulation effect on physical fatigue patients. In addition, most of the physical fatigue syndrome patients need long-term treatment and recuperation, and the pure natural Chinese herbal medicine with the health care function has mild medicine property, is not easy to generate medicine resistance and is suitable for long-term administration.
Therefore, the development of an anti-fatigue traditional Chinese medicine compound composition which is efficient, safe, mild in medicine property, wide in applicable population and suitable for long-term administration is a technical problem which needs to be solved by technical personnel in the field urgently.
Disclosure of Invention
The inventor thinks that the fatigue belongs to the category of 'fatigue' and 'consumptive disease' in the traditional Chinese medicine. Fatigue refers to excessive exertion and mental stress; consumptive disease is also called as deficiency and refers to the general term of various chronic diseases caused by deficiency of primordial qi and deficiency of the zang-fu organs. The traditional Chinese medicine considers that the qi and blood of a human body are easily injured by fatigue caused by excessive exercise. The book Su Wen & Tao Tong Lun mentions that "qi exhaustion occurs due to overstrain, dyspnea with sweat occurs due to overstrain, both internally and externally, so qi exhaustion occurs. "Su Wen & Shang Gu Tian Zhen Lun" also has cloud "the form is tired but not tired, and qi is flowing smoothly", and if the form is tired or tired too much, it can consume qi and be deficient. "liver stores blood" in Su Wen, Tiaojing Lun "means that liver has the function of storing and regulating blood volume, when the human body is moving, the liver supplies the stored blood to the whole body, so that the brain or limbs can fully exert their strength without fatigue. The "liver is the root of the strike", i.e. the liver is the root of fatigue resistance, as described in Su Wen, Lian Ji Bian Lun. The liver governs tendons and tendons depend on blood to nourish them, which fills the tendons and governing fascia of the body. Kidneys store essence, which is the innate root, governs bones and produces marrow, and leads to brain, and is the source of physical strength and motive power for producing skill. The kidney essence is sufficient, so that the user can not only shake and feel mentally and agile, but also have strong muscles and bones and powerful actions; otherwise, soreness and weakness of the waist and back, weak bones, dizziness, etc. Therefore, physical fatigue is especially closely related to liver and kidney, and can damage qi and blood.
The inventor selects traditional Chinese medicines with mild drug properties as raw materials, medlar as monarch drug, ginseng as ministerial drug, epimedium and glossy privet fruit as innovative formula of assistant and guide drugs, according to the compatibility theory of monarch, ministerial, assistant and guide drugs in traditional Chinese medicine, and scientifically verifies the safety and effectiveness of the formula by applying modern pharmacological theory and research results. Experiments prove that the anti-fatigue traditional Chinese medicine composition provided by the invention has the advantages of high efficiency, safety, wide applicable population, mild medicine property, suitability for long-term administration and the like.
The anti-fatigue traditional Chinese medicine composition is mainly prepared on the basis of the following conception:
wolfberry fruit: the tea is sweet in taste and neutral in nature, enters liver and kidney channels, has the effects of tonifying kidney and replenishing essence, and nourishing liver to improve eyesight, and is considered by the theory of traditional Chinese medicine: "kidney stores essence", the kidney person is skilled and has appeared, the essence is deficient after consumption, wolfberry fruit can tonify kidney essence to cure internal injury, and "Shennong Bencao Jing" in Shen nong's herbal, it is taken for a long time, it can strengthen muscles and bones, lighten body, prevent aging, resist cold and summer heat ", is listed as the top grade. The book Ben Cao Hui Yan (the classic but know that qi tonifies must use ginseng and astragalus, blood tonics must use Gui and Di, yang tonics must use Gui and Fu, yin tonics must use Zhi and Bai, fire-reducing must use Qin Lian, dampness-dispersing must use cang and Pu, wind-dispelling must use Qiang, Du and Fang Feng, while unknown medlar can make qi full, blood tonics, yang can grow, yin can grow, fire can reduce, wind-damp can get away, there are ten times of wonderful use), is the monarch drug of the recipe.
Ginseng: sweet taste, slight bitter taste and mild nature, and has the effects of invigorating primordial qi, restoring pulse, relieving depletion, invigorating spleen, benefiting lung, promoting fluid production, and quenching thirst. The Shen nong Ben Cao Jing (Shen nong's herbal medicine) listed as the superior one, said it has the effects of nourishing five internal organs, calming the mind, calming the soul, stopping palpitation, improving eyesight, benefiting intelligence, reducing weight and prolonging life after being taken for a long time, so it is a ministerial medicine of the recipe. The medlar and the ginseng have the effects of replenishing essence, tonifying qi, tonifying essence and recovering deficiency, and are the monarch and minister medicines of the formula respectively.
Herba epimedii and glossy privet fruit: herba Epimedii is pungent and sweet in flavor, neutral in nature, and enters liver and kidney meridians, and has effects of invigorating kidney yang, and strengthening tendons and bones. The glossy privet fruit is sweet and bitter in taste, neutral in nature, enters liver and kidney meridians, and has the effect of nourishing liver and kidney. Together, they are the adjuvant and guiding drugs of the recipe.
The invention takes the medlar as the monarch drug, tonifies the liver and the kidney, and benefits the essence and the blood; ginseng is effective in invigorating primordial qi, restoring pulse, relieving depletion, invigorating spleen, benefiting lung, promoting fluid production, and quenching thirst. The medlar and the ginseng, one supplement essence and one supplement qi, are the monarch drug and the ministerial drug of the formula respectively; herba Epimedii is effective in invigorating kidney yang, and strengthening tendons and bones; fructus Ligustri Lucidi is effective in nourishing liver and kidney; the epimedium and the glossy privet fruit, one of which is used for tonifying yang and the other is used for tonifying yin and yang, are used together as adjuvant and guiding drugs of the recipe. The anti-fatigue traditional Chinese medicine composition disclosed by the invention is prepared from the four medicines of wolfberry fruit, ginseng, epimedium and glossy privet fruit, has the effects of nourishing liver and kidney, benefiting yin and supporting yang together, achieves the purpose of relieving physical fatigue, is mild and safe in medicine property, and is suitable for being taken by people who are easy to fatigue for a long time.
Particularly, the invention creatively increases the dosage of adjuvant drugs, namely the glossy privet fruit, and aims to improve the effects of nourishing liver and kidney and eliminating soreness and weakness of waist and knees, and to supplement yin essence of people by combining with the medlar so as to help the monarch drugs to take effect quickly.
Particularly, the invention creatively increases the dosage of the adjuvant drug epimedium herb, and aims to strengthen the muscles and bones, tonify the waist and knees and strengthen the heart force so as to benefit the mild and lasting effect of the whole formula, and also can supplement the yang qi of people by combining with ginseng so as to benefit the yang-strengthening effect of ministerial drugs.
Particularly, the invention creatively uses epimedium and glossy privet fruit in the same amount, one is the yang of tonifying the kidney, the other is the yin of tonifying the kidney, and the balance and the complementation of yin and yang are realized to supplement the essence and qi of the kidney.
In particular, the amount of the solvent to be used,
the invention provides a traditional Chinese medicine composition, which can efficiently and safely relieve physical fatigue, has the advantages of mild medicine property, wide applicable population, suitability for long-term taking and the like, and is prepared from the following traditional Chinese medicines in parts by weight:
wherein, the medlar is a monarch drug, the ginseng is a ministerial drug, and the epimedium and the glossy privet fruit are an assistant drug.
Wherein, the weight ratio of the epimedium to the glossy privet fruit is preferably 1: 1.
Preferably, the invention provides an anti-fatigue traditional Chinese medicine composition which is prepared from the following raw materials in parts by weight:
wherein fructus Lycii is dried mature fruit of Lycium barbarum L of Solanaceae. Harvesting fruits in summer and autumn when the fruits are red, drying the fruits until the fruits are wrinkled, drying the fruits in the sun, and removing fruit stalks.
Wherein the Ginseng radix product is dried root and rhizome of Panax ginseng C.A. Meyer of Araliaceae. Collected in autumn, washed, dried in the sun or baked.
Wherein the herba Epimedii is dried aerial part of herba Epimedii of berberidaceae. Collecting and cutting in summer and autumn when the stems and leaves are flourishing, removing coarse stems and impurities, and sun drying or shade drying.
Wherein fructus Ligustri Lucidi is dried mature fruit of fructus Ligustri Lucidi of Oleaceae. Harvesting in winter, removing branches and leaves, steaming or slightly scalding in boiling water, and drying; or directly dried.
Further, the invention also relates to a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
1) reflux-extracting herba Epimedii and fructus Ligustri Lucidi with organic solvent, filtering the extractive solution, recovering organic solvent from the filtrate, and concentrating to obtain fluid extract A;
2) decocting fructus Lycii in water, filtering decoction, and concentrating the filtrate to obtain fluid extract B;
3) mixing fluid extract A and fluid extract B, drying, and pulverizing into dry extract powder;
4) drying Ginseng radix, and pulverizing into Ginseng radix powder, or directly weighing Ginseng radix powder;
5) and mixing the dry paste powder and the ginseng powder uniformly to obtain the traditional Chinese medicine composition.
Preferably, the traditional Chinese medicine composition is an anti-fatigue traditional Chinese medicine composition.
Preferably, the dry paste powder and the ginseng powder all pass through a No. five sieve, and the powder which can pass through a No. six sieve is not less than 95 percent.
Preferably, in the step 1), the epimedium and the glossy privet fruit are refluxed and extracted at least 2 times, 5-15 times of solvent (more preferably 9 times of solvent) is added each time, the reflux extraction is carried out for 1-3 hours (more preferably 2 hours), the extracting solution is filtered (the medicine residues are stored in another container), the filtrates are combined, the organic solvent is recovered from the filtrate (an organic solvent recovery tower, 0.065-0.075Mpa, 60-65 ℃) and concentrated to the clear paste A with the relative density of 1.10-1.35(60 ℃), preferably 1.10-1.25(60 ℃), more preferably 1.10-1.20 (60 ℃); wherein, the filtering step adopts a sieve with 150 meshes and 300 meshes, and more preferably a sieve with 200 meshes.
Preferably, wherein the organic solvent in step 1) is ethanol.
Preferably, the medlar in the step 2) and the dregs after the reflux extraction in the step 1) are decocted together by adding water for at least 2 times, 8-12 times of water (more preferably 10 times of water) is added each time, the decoction is decocted for 1-2 hours (more preferably 1.5 hours), the decoction is filtered (150 meshes and 300 meshes, more preferably 200 meshes), the filtrates are combined, and the filtrate is decompressed and concentrated to a clear paste B with the relative density of 1.10-1.35(60 ℃), preferably 1.10-1.25(60 ℃), more preferably 1.10-1.20 (60 ℃).
Preferably, the drying of the clear paste A and the clear paste B in the step 3) is decompression drying under the conditions of-0.085 Mpa and 65 ℃; the dry extract powder is preferably sieved by a 100-mesh sieve.
Preferably, wherein the drying temperature of the ginseng in the step 4) is 60 ℃, and the ginseng powder is preferably sieved by a 100-mesh sieve; or preferably sterilizing the ginseng powder by irradiation, wherein the irradiation condition is preferably 5kGy dose60Co。
Further, the invention also relates to a traditional Chinese medicine preparation, which can be prepared into other dosage forms such as capsules, granules, tablets, pills, powder or oral liquid according to the conventional preparation technology in the field. For example: for capsules, the traditional Chinese medicine composition can be prepared into soft materials by using 85% ethanol, granulation is carried out by using a 16-mesh sieve, drying is carried out at 55-60 ℃, granules are granulated by using a 14-mesh sieve, 0.4 g/granule of the granules are filled into No. 0 capsules, and the capsules can be prepared by polishing, inspecting and packaging.
Furthermore, the invention also relates to application of the traditional Chinese medicine composition or the traditional Chinese medicine preparation in preparing anti-fatigue products, and the products are preferably medicines or health-care foods.
The recommended dosage of the traditional Chinese medicine preparation capsule is as follows: orally taken 2 times/day, 3 capsules (1.2g) each time, or following the prescription.
The traditional Chinese medicine composition of the invention has the following beneficial effects:
(1) experiments show that the anti-fatigue traditional Chinese medicine composition takes liver and kidney nourishing, qi benefiting and blood nourishing as rules of treatment, the four medicines of the medlar, the ginseng, the epimedium herb and the glossy privet fruit are combined to play the effects of liver and kidney nourishing, yin benefiting and yang supporting, the purpose of physical fatigue relieving is achieved, the medicine property is mild and safe, the cheap and easily available medlar is taken as a monarch medicine, the anti-fatigue effect is greatly increased under the assistance of an adjuvant and a guiding medicine, and the anti-fatigue traditional Chinese medicine composition is mild and is suitable for being taken by people who are easy to fatigue for a long time.
(2) Experiments show that compared with a composition (comparison example 1) which takes ginseng as a monarch drug, medlar as a ministerial drug, epimedium and glossy privet fruit as an assistant and guide drug, a composition (comparison example 2) which takes epimedium as a monarch drug, glossy privet fruit as a ministerial drug, medlar and ginseng as an assistant and guide drug, a composition (comparison example 3) which takes epimedium and glossy privet fruit as a monarch drug, ginseng as a ministerial drug and medlar as an assistant and guide drug, the traditional Chinese medicine composition (examples 1-4) which takes medlar as a monarch drug, ginseng as a ministerial drug and epimedium and glossy privet fruit as an assistant and guide drug has obvious advantages in the aspects of medication safety, effectiveness, applicable crowd range and the like. Namely, compared with other prescription strategies or concepts, the prescription mainly has the effects of tonifying qi and soothing the nerves, nourishing the liver and kidney, and promoting the production of body fluid and nourishing the blood (examples 1 to 4) is safer and more effective in the aspect of improving the immunity of the organism and has a wider application range.
(3) The invention creatively increases the dosage of the assistant and guide medicaments glossy privet fruit and epimedium herb, and leads the glossy privet fruit and the epimedium herb to clear up soreness and weakness of waist and knees, strengthen muscles and bones, tonify waist and knees and strengthen heart force while assisting the monarch medicament medlar and ministerial medicament ginseng to exert the effects, so that the anti-fatigue effect of the composition is mild, the applicable crowd is wide, and the glossy privet fruit and the epimedium herb are used approximately in equal amount, so that the yin and yang are balanced and supplemented, the curative effect is lasting and high-efficiency.
(4) Ginseng is rare and powdery. The traditional Chinese medicine composition of the invention takes the ginseng powder as the medicine, can be used as an excipient, avoids moisture absorption and agglomeration of capsule contents, and can retain the effective components to the maximum extent; not only improves the stability of the product, but also reduces the use of auxiliary materials and reduces the production cost.
Detailed Description
The technical solution of the present invention will be described in detail with reference to specific examples and comparative examples. The traditional Chinese medicine raw materials used by the invention are purchased from Xijingzhi medicine Limited company of Xian algae Lutang pharmaceutical industry group.
Examples
EXAMPLE 1 preparation of the Chinese medicinal composition of the present invention
(1) Extracting herba epimedii 3.0kg and glossy privet fruit 3.5kg with ethanol under reflux for 3 times, adding 7 times of ethanol each time, extracting under reflux for 2 hours each time, filtering the extract (with a 200-mesh stainless steel sieve, storing the residue in another container), merging the filtrates, recovering ethanol, and concentrating under reduced pressure to obtain a clear paste A with a relative density of 1.10-1.20 (60 ℃);
(2) decocting 5.0kg of wolfberry fruit and the dregs of the reflux extraction in the step 1) with 8 times of water for 2 times, decocting for 1.5 hours each time, filtering decoction liquid (200-mesh stainless steel sieve), merging filtrate, and concentrating under reduced pressure to obtain clear paste B with the relative density of 1.10-1.20 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) drying Ginseng radix 2.0kg at 60 deg.CAfter drying, pulverizing, sieving with a 100 mesh sieve, sterilizing by irradiation (5kGy,60co) to obtain ginseng powder;
(5) mixing the above dry extract powder and Ginseng radix powder to obtain 3.48kg of the Chinese medicinal composition.
EXAMPLE 2 preparation of the Chinese medicinal composition of the present invention
(1) Extracting herba epimedii 3.5kg and glossy privet fruit 4.5kg with ethanol under reflux for 2 times, adding 9 times of ethanol each time, extracting under reflux for 2 hours each time, filtering the extract (with a 200-mesh stainless steel sieve, storing the residue in another container), merging the filtrates, recovering ethanol, and concentrating under reduced pressure to obtain a clear paste A with a relative density of 1.20-1.25 (60 ℃);
(2) decocting 4.0kg of medlar and the dregs of a decoction obtained after reflux extraction in the step 1) with water for 2 times, adding 10 times of water each time, decocting for 1.5 hours each time, filtering decoction liquid (a 200-mesh stainless steel sieve), merging filtrate, and concentrating under reduced pressure (-0.065-0.075 Mpa, 75 ℃) to obtain clear paste B with the relative density of 1.20-1.25 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) 1.5kg of ginseng is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, sterilized by irradiation (5kGy,60co) to obtain ginseng powder;
(5) mixing the above dry extract powder and Ginseng radix powder to obtain 2.85kg of the Chinese medicinal composition.
EXAMPLE 3 preparation of the Chinese medicinal composition of the present invention
(1) Extracting herba epimedii 4.5kg and glossy privet fruit 5.0kg with ethanol under reflux for 3 times, adding 10 times of ethanol each time, extracting under reflux for 2 hours each time, filtering the extract (with a 200-mesh stainless steel sieve, storing the residue in another container), merging the filtrates, recovering ethanol, and concentrating under reduced pressure to obtain a clear paste A with a relative density of 1.10-1.20 (60 ℃);
(2) decocting 3.0kg of medlar and the dregs of a decoction obtained after reflux extraction in the step 1) with water for 3 times, adding 12 times of water each time, decocting for 1.5 hours each time, filtering decoction liquid through a stainless steel sieve of 200 meshes, merging filtrate, and concentrating under reduced pressure to obtain clear paste B with the relative density of 1.10-1.20 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) 1.0kg of ginseng is taken, dried at 60 ℃, crushed, sieved by a 100-mesh sieve, sterilized by irradiation (5kGy,60co) to obtain ginseng powder;
(5) mixing the above dry extract powder and Ginseng radix powder to obtain 2.41kg of the Chinese medicinal composition.
EXAMPLE 4 preparation of the Chinese medicinal composition of the present invention
(1) Extracting herba epimedii 4.0kg and glossy privet fruit 4.0kg with ethanol under reflux for 2 times, adding 13 times of ethanol each time, extracting under reflux for 2 hours each time, filtering the extract (with a 200-mesh stainless steel sieve, storing the residue in another container), merging the filtrates, recovering ethanol, and concentrating under reduced pressure to obtain a clear paste A with a relative density of 1.30-1.35 (60 ℃);
(2) decocting 4.0kg of wolfberry fruit and the dregs obtained in the reflux extraction in the step 1) with water for 3 times, adding 10 times of water for each time, decocting for 1.5 hours, filtering decoction liquid (200-mesh stainless steel sieve), merging filtrate, and concentrating under reduced pressure to obtain clear paste B with the relative density of 1.30-1.35 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) mixing the above dry extract powder with 1.5kg Ginseng radix powder (sieved with 100 mesh sieve) to obtain 2.85kg of the Chinese medicinal composition.
Example 5 preparation of capsules of the Chinese medicinal composition of the present invention
Taking 3.48kg of the traditional Chinese medicine composition prepared in the embodiment 1, preparing a soft material by using 85% ethanol, granulating by using a 16-mesh sieve, drying at 55-60 ℃, grading by using a 14-mesh sieve, filling 0.4 g/capsule into a No. 0 capsule, polishing, inspecting and packaging to obtain 9545 capsules of the traditional Chinese medicine composition.
Comparative example
Control example 1 preparation of control Chinese medicinal composition
(1) Extracting herba epimedii 2.5kg and glossy privet fruit 3.0kg with ethanol under reflux for 2 times, adding 9 times of ethanol each time, extracting under reflux for 2 hours each time, filtering the extract (200-mesh stainless steel sieve, storing the residue in another container), merging the filtrates, recovering ethanol, and concentrating under reduced pressure (-0.065 to-0.075 Mpa at 75 ℃) to obtain clear paste A with the relative density of 1.10 to 1.20(60 ℃);
(2) decocting 3.0kg of medlar and the dregs of a decoction obtained after reflux extraction in the step 1) with water for 2 times, adding 10 times of water each time, decocting for 1.5 hours each time, filtering decoction liquid (a 200-mesh stainless steel sieve), merging filtrate, and concentrating under reduced pressure (-0.065-0.075 Mpa, 75 ℃) to obtain clear paste B with the relative density of 1.10-1.20 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) collecting Ginseng radix 5.0kg, drying at 60 deg.C, pulverizing, sieving with 100 mesh sieve, sterilizing by irradiation (5kGy,60co) to obtain ginseng powder;
(5) mixing the above dry extract powder and Ginseng radix powder to obtain 5.78kg of the Chinese medicinal composition.
Control example 2 preparation of control Chinese medicinal composition
(1) Taking 6.5kg of epimedium herb and 3.5kg of glossy privet fruit, adding ethanol for reflux extraction for 3 times, adding 9 times of ethanol for each time, reflux extracting for 2 hours for each time, filtering an extracting solution (a 200-mesh stainless steel sieve, storing dregs of a decoction in another container), merging the filtrates, recovering the ethanol, concentrating under reduced pressure (-0.065 to-0.075 Mpa at 75 ℃) to obtain a clear paste with the relative density of 1.20 to 1.25(60 ℃), drying the clear paste under vacuum (-0.085Mpa at 65 ℃), crushing, and sieving by a 80-mesh sieve to obtain dry paste powder;
(2) taking 1.5kg of medlar, 4.5kg of epimedium herb and 4.5kg of glossy privet fruit, drying at 60 ℃, crushing, sieving with a 100-mesh sieve, irradiating for sterilization (5kGy,60co) to obtain mixed powder of the medlar, the epimedium and the glossy privet fruit;
(5) and mixing the above dry extract powder and mixed powder to obtain 11.06kg of the Chinese medicinal composition.
Control example 3 preparation of control Chinese medicinal composition
(1) Taking 3.0kg of ginseng, adding ethanol for reflux extraction for 3 times, adding 9 times of ethanol for each time, reflux extracting for 2 hours for each time, filtering an extracting solution (a 200-mesh stainless steel sieve and storing dregs in another container), merging filtrates, recovering ethanol, and concentrating under reduced pressure to obtain a clear paste A with the relative density of 1.30-1.35 (60 ℃);
(2) decocting 1.5kg of medlar and the dregs of a decoction obtained after reflux extraction in the step 1) with water for 3 times, adding 10 times of water for each time, decocting for 1.5 hours, filtering decoction liquid through a stainless steel sieve of 200 meshes, merging filtrate, and concentrating under reduced pressure to obtain clear paste B with the relative density of 1.30-1.35 (60 ℃);
(3) mixing fluid extract A and fluid extract B, vacuum drying (-0.085Mpa, 65 deg.C), pulverizing, and sieving with 80 mesh sieve to obtain dry extract powder;
(4) taking 4.5kg of epimedium herb and 4.5kg of glossy privet fruit, drying and crushing at 60 ℃, sieving by a 100-mesh sieve, irradiating and sterilizing (5kGy,60co) to obtain mixed powder of epimedium and glossy privet fruit;
(5) mixing the above dry extract powder, herba Epimedii and fructus Ligustri Lucidi powder, and making into 9.22kg of the Chinese medicinal composition.
Examples of the experiments
Taking 320 male Kunming mice (provided by Experimental animals center of Chengdu biological products research institute, Limited liability company, with the weight of 18g-22g), randomly dividing the mice into 4 batches (80 mice in each batch) and 8 groups (10 mice in each group), namely 4 experimental groups, 3 control groups and 1 blank control group, preparing a test object by using distilled water as a solvent, and respectively carrying out oral gavage on the mice according to the ratio of 20ml/kg.BW once a day for 30 days continuously. The preparation method, administration mode, dosage and the like of the test solution of each group are shown in the following table:
experimental example 1 weight swimming test
After the animals of each group of the experiment are given to the test object for 30min in the last time, the tail root of the rat is loaded with a thin fuse with the weight of 5%, then the mouse is put into a swimming box with the water temperature of 25 +/-1.0 ℃ and the water depth of 30cm for swimming, the time from the swimming to the death of the mouse is recorded, the swimming time of the mouse is calculated, and the results are as follows:
| dosage (mg/kg. BW) | Animal number (only) | Swimming time (min) |
| Experimental group 1 | 10 | 27.20±7.02 |
| Experimental group 2 | 10 | 25.40±7.33 |
| Experimental group 3 | 10 | 26.70±5.38 |
| Experimental group 4 | 10 | 26.00±7.72 |
| Control group 1 | 10 | 22.40±7.43 |
| Control group 2 | 10 | 21.70±5.38 |
| Control group 3 | 10 | 23.00±7.14 |
| Blank control group | 10 | 17.10±4.65 |
It can be seen that the swimming time of the control group mice is longer than that of the blank control group, but shorter than that of the positive control group; the swimming time of the experimental group of mice is obviously longer than that of the control group and the positive control group. Therefore, the traditional Chinese medicine composition can remarkably prolong the swimming time of mice.
EXAMPLE 2 serum Urea assay
After two groups of animals are tested and the tested object is given for 30min in the last time, the mice are put into water with the temperature of 30 ℃ for swimming for 90min without load, after the mice are rested for 60min, 0.5ml of whole blood is collected by drawing eyeballs, the serum is taken according to the instruction of a kit, and the content of the serum urea is measured by a CX4 full-automatic biochemical analyzer and the kit provided by Beckmann Kulter Co., Ltd, USA, the results are as follows:
| dosage (mg/kg. BW) | Animal number (only) | Serum urea (mmol/L) |
| Experimental group 1 | 10 | 4.43±0.65 |
| Experimental group 2 | 10 | 4.32±0.58 |
| Experimental group 3 | 10 | 4.61±0.47 |
| Experimental group 4 | 10 | 4.55±0.49 |
| Control group 1 | 10 | 5.50±0.51 |
| Control group 2 | 10 | 5.31±0.34 |
| Control group 3 | 10 | 5.40±0.42 |
| Blank control group | 10 | 5.53±0.55 |
Thus, the control traditional Chinese medicine composition hardly affects the serum urea level of the mice, and is similar to the blank control group; the traditional Chinese medicine composition (experimental groups 1-4) can obviously reduce the serum urea level of mice.
Experimental example 3 liver glycogen assay
After three groups of animals were administered with test substance for 30min, killing animal, taking liver, rinsing with physiological saline, blotting with filter paper, weighing about 100mg liver, and determining liver glycogen content according to anthrax method, which has the following results:
| dosage (mg/kg. BW) | Animal number (only) | Hepatic glycogen (mg/100g liver) |
| Experimental group 1 | 10 | 5271.23±1001.63 |
| Experimental group 2 | 10 | 5175.51±1025.94 |
| Experimental group 3 | 10 | 5657.60±1106.65 |
| Experimental group 4 | 10 | 5287.99±835.27 |
| Control group 1 | 10 | 4371.82±1100.16 |
| Control group 2 | 10 | 4354.65±996.28 |
| Control group 3 | 10 | 4087.12±1018.73 |
| Blank control group | 10 | 3717.15±913.01 |
It can be seen that liver glycogen levels were increased in the control mice compared to the blank control group; the traditional Chinese medicine composition (experimental groups 1-4) provided by the invention can be used for remarkably improving the glycogen level of mice.
Claims (9)
1. An anti-fatigue traditional Chinese medicine composition comprises the following components in parts by weight:
3-5 parts of wolfberry fruit,
1-2 parts of ginseng (Panax ginseng C.A.Meyer),
3-5 parts of epimedium herb, namely,
3-5 parts of glossy privet fruit;
the preparation method of the traditional Chinese medicine composition comprises the following steps:
1) reflux-extracting herba Epimedii and fructus Ligustri Lucidi with ethanol, filtering the extractive solution, recovering ethanol from the filtrate, and concentrating to obtain fluid extract A;
2) decocting the medlar and the dregs obtained after the reflux extraction in the step 1) with water, filtering decoction, and concentrating the filtrate into clear paste B;
3) mixing fluid extract A and fluid extract B, drying, and pulverizing into dry extract powder;
4) drying Ginseng radix, and pulverizing into Ginseng radix powder, or directly weighing Ginseng radix powder;
5) and mixing the dry paste powder and the ginseng powder uniformly to obtain the traditional Chinese medicine composition.
2. The Chinese medicinal composition according to claim 1, wherein the wolfberry fruit is monarch drug, the ginseng is ministerial drug, and the epimedium and the glossy privet fruit are assistant drugs.
3. A method for preparing the traditional Chinese medicine composition of claim 1 or 2, comprising the following steps:
1) reflux-extracting herba Epimedii and fructus Ligustri Lucidi with ethanol, filtering the extractive solution, recovering ethanol from the filtrate, and concentrating to obtain fluid extract A;
2) decocting the medlar and the dregs obtained after the reflux extraction in the step 1) with water, filtering decoction, and concentrating the filtrate into clear paste B;
3) mixing fluid extract A and fluid extract B, drying, and pulverizing into dry extract powder;
4) drying Ginseng radix, and pulverizing into Ginseng radix powder, or directly weighing Ginseng radix powder;
5) and mixing the dry paste powder and the ginseng powder uniformly to obtain the traditional Chinese medicine composition.
4. The preparation method according to claim 3, wherein step 1) herba Epimedii and fructus Ligustri Lucidi are reflux-extracted at least 2 times.
5. The preparation method according to claim 3, wherein the relative density of the fluid extract in steps 1) and 2) is 1.10-1.35.
6. The method according to claim 3, wherein the dry extract powder and ginseng powder are powders which pass through a five-mesh sieve and contain not less than 95% of powder passing through a six-mesh sieve.
7. A Chinese medicinal preparation made from the Chinese medicinal composition of claim 1 or 2.
8. The Chinese medicinal preparation according to claim 7, which is a capsule, granule, tablet, pill, powder or oral liquid.
9. Use of the Chinese medicinal composition of claim 1 or 2 for preparing an anti-fatigue medicament or a health food.
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