CN112617023B - Composition containing feather protein powder and preparation method thereof - Google Patents
Composition containing feather protein powder and preparation method thereof Download PDFInfo
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- CN112617023B CN112617023B CN202011546686.6A CN202011546686A CN112617023B CN 112617023 B CN112617023 B CN 112617023B CN 202011546686 A CN202011546686 A CN 202011546686A CN 112617023 B CN112617023 B CN 112617023B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/163—Sugars; Polysaccharides
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/20—Inorganic substances, e.g. oligoelements
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a composition containing feather protein powder and a preparation method thereof. The composition containing the feather protein powder is prepared from corn flour, the feather protein powder, prebiotics, multivitamins, amino acid, bone meal, calcium carbonate, bracken, salt, an additive and water. The composition containing the feather protein powder provided by the invention covers comprehensive nutrition required by animal growth, supplements calcium element, promotes calcium absorption, promotes animal growth and is easy to digest and absorb by animals. In addition, the composition containing the feather protein powder provided by the invention contains nutritional ingredients such as prebiotics, bracken, ferulic acid and the like, and can promote intestinal peristalsis of animals, regulate immunity and reduce the morbidity of the animals.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a composition containing feather protein powder and a preparation method thereof.
Background
With the improvement of living standard of people, the demand of meat products is continuously improved, so that the feed industry is rapidly developed in the past decades, and the demand of feed raw materials is greatly improved. However, China is a country with deficient protein resources, protein raw materials of conventional feed comprise soybean meal, fish meal and the like, and mainly depend on imported protein, and although the imported protein has good effect, the imported protein is expensive, so that the production cost of the feed is greatly increased.
The animal feed in the market is of various types, and the formulas and components of various animal feeds are different. The traditional animal feed is a formula feed prepared by compounding grain additives, the nutrition absorbed by animals is insufficient, and the risk of animal diseases is extremely high. The feeding effect of the current commercial feeds is greatly different, most animal feeds are added with additives in various forms, and the animals eat the feeds for a long time to show various side effects physiologically and have disadvantages to the animal bodies.
Patent publication No. CN112042823A discloses a pig feed prepared from field weeds and a preparation method thereof, wherein the feed comprises the following formula components of field weeds, ingredients and refined materials, wherein the field weeds comprise sowthistle herbs, hollow lotus seeds, fish spears, clarified grass, chickweed and speedwell. The feed does not contain chemical additives, can improve the lean meat percentage of pork and reduce the breeding cost, but has small promotion effect on the growth of pigs.
With the rapid development of the large-scale poultry industry, more and more feathers are discarded every year, which not only causes a series of problems of environmental pollution, but also causes a great deal of waste of protein sources. The feather powder is prepared from various poultry feathers as raw materials, has high protein content, is rich in various amino acids such as glycine, serine, cystine, isoleucine and the like, and is one of effective sources for solving the problem of feed protein source tension. However, feather protein contained in feather meal is a highly cross-linked keratin composed of disulfide bonds, and is not easily degraded by general proteases such as pepsin and trypsin, so that it is difficult to directly digest and absorb feather protein by animals, and pretreatment thereof is required.
Patent publication No. CN108707640A discloses a water-soluble enzymolysis feather protein powder and a preparation method thereof, wherein the preparation process of the feather protein powder comprises the steps of cooking pretreatment, pulping, enzymolysis, filtering, concentration, drying and the like. Although the feather protein powder can be obtained by the method, the yield of the feather protein is low, and the amino acid in the feathers is protected by nitrogen in the cooking process, but the protein source waste caused by the denaturation of part of the amino acid due to high-temperature cooking cannot be avoided.
In conclusion, the technical problems that the feed formula is unreasonable, the growth of animals cannot be promoted, the nutrient components are incomplete, feather protein cannot be completely utilized and the like generally exist in the prior art.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a composition containing feather protein powder and a preparation method thereof. The composition containing the feather protein powder provided by the invention is reasonable in formula, covers all nutrients required by animal growth, and supplements calcium element, promotes calcium absorption and promotes animal growth on the basis. In addition, the feather protein powder in the composition containing the feather protein powder is easy to digest and absorb by animals, and other protein sources are not required to be additionally added when the composition is applied, so that the technical problem of shortage of the protein source is effectively solved.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a composition containing feather protein powder comprises the following components in parts by weight:
50-70 parts of corn flour, 35-55 parts of feather protein powder, 10-20 parts of prebiotics, 5-15 parts of multivitamins, 5-15 parts of amino acid, 20-30 parts of bone meal, 10-20 parts of calcium carbonate, 15-30 parts of bracken, 1-5 parts of salt, 15-25 parts of additives and 10-20 parts of water.
Further, the composition containing the feather protein powder comprises the following components in parts by weight: 62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
Further, the preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: the method comprises the steps of dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.0-1.5h at the temperature of 200-250 ℃ and under the pressure of 10-15Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather powder.
Furthermore, the prebiotics in the composition containing the feather protein powder comprise lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 15-18:5-7: 11-13.
Furthermore, the compound vitamin in the composition containing the feather protein powder consists of vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is composed of 3-5:9-11:7-10:1-3:12-15 by mass ratio.
Furthermore, the amino acid in the composition containing the feather protein powder consists of lysine, methionine, tryptophan, histidine, isoleucine, leucine, phenylalanine, valine, threonine and arginine in a mass ratio of 3:1:5:4:9:7:2:6:11: 8.
Furthermore, the additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 22-25:13-16: 19-21.
The invention also provides a preparation method of the composition containing the feather protein powder, which comprises the following steps:
s1, uniformly mixing corn flour, feather protein powder, prebiotics, compound vitamins, bone meal and calcium carbonate to obtain a mixture;
s2, putting the fiddlehead into a grinder, adding water, and grinding into slurry;
s3, mixing the mixture obtained in the step S1 with the slurry obtained in the step S2, adding amino acid, salt and additives, uniformly stirring, introducing into a granulator with the caliber of a ring die of 5.0mm, and carrying out extrusion granulation to obtain granules;
s4, drying the granules prepared in the step S3, cooling, sieving to remove powder, and packaging to obtain the finished product.
The feather protein powder in the composition containing the feather protein powder provided by the invention is subjected to high temperature action instantly in puffing under the high temperature and high pressure of 200-250 ℃ and the shearing action of a puffing machine under the pressure of 10-15Mpa, the firm spatial structure of the keratin is destroyed when the feather is subjected to decompression and puffing at a die hole, and disulfide bonds are broken, so that the keratin fiber is changed into smaller protein subunits and peptide chain groups arranged in a linear mode, and thus, the feather protein powder is easy to digest and absorb by animals.
The prebiotics in the formula of the invention is used as a nutritional supplement for enhancing immunoregulation and effectively resisting inflammation, can enhance the animal constitution, improve the immunity, effectively resist allergy and inflammation reaction, improve the organism recovery capability after diseases and reduce the infection risk. The research of the invention finds that when the prebiotics consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve according to a certain mass ratio, the prebiotics play the best role in enhancing the immunoregulation.
The bone meal and the calcium carbonate in the formula jointly form a calcium supplement component of the composition containing the feather protein powder, so that the slow growth of animals caused by calcium deficiency is prevented, and the animals are helped to grow quickly. The fiddlehead contains nutrient substances and mineral substances such as carotene, vitamins, protein, fat, dietary fiber, potassium, calcium, magnesium and the like, can meet the growth requirements of animals, can play a positive synergistic effect with prebiotics, and can promote intestinal tract movement, help the animals to digest and absorb and reduce the gastrointestinal diseases of the animals. Meanwhile, the pteridium aquilinum element contained in the pteridium aquilinum has certain inhibition effect on pathogenic bacteria, and can reduce the disease rate of animals. The ferulic acid in the additive has physiological effects of scavenging free radicals, improving immunity, resisting bacteria, diminishing inflammation, reducing blood fat, reducing stress and the like, can be absorbed and eliminated by organisms, can not be accumulated in the bodies, has almost no toxic effect, and is an ideal green additive.
Compared with the prior art, the composition containing feather protein powder and the preparation method thereof provided by the invention have the following advantages:
(1) the composition containing the feather protein powder provided by the invention is reasonable in formula, covers all nutrients required by animal growth, and supplements calcium element, promotes calcium absorption and promotes animal growth on the basis;
(2) the feather protein powder in the composition containing the feather protein powder is easy to digest and absorb by animals, and other protein sources are not required to be additionally added when the composition is applied, so that the technical problem of shortage of the protein source is effectively solved;
(3) the composition containing the feather protein powder provided by the invention contains nutritional ingredients such as prebiotics, bracken, ferulic acid and the like, and can promote intestinal peristalsis of animals, accelerate feeding, regulate immunity and reduce the illness rate of the animals.
Detailed Description
The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples. Various modifications can be made by those skilled in the art based on the basic idea of the invention, but it is within the scope of the invention as long as it does not depart from the basic idea of the invention.
The amino acid in the composition containing feather protein powder in the embodiment consists of lysine, methionine, tryptophan, histidine, isoleucine, leucine, phenylalanine, valine, threonine and arginine in a mass ratio of 3:1:5:4:9:7:2:6:11: 8.
The preparation method of the composition containing the feather protein powder specifically comprises the following steps:
s1, uniformly mixing corn flour, feather protein powder, prebiotics, compound vitamins, bone meal and calcium carbonate to obtain a mixture;
s2, putting the fiddlehead into a grinder, adding water, and grinding into slurry;
s3, mixing the mixture obtained in the step S1 with the slurry obtained in the step S2, adding amino acid, salt and additives, uniformly stirring, introducing into a granulator with the caliber of a ring die of 5.0mm, and carrying out extrusion granulation to obtain granules;
s4, drying the granules prepared in the step S3, cooling, sieving to remove powder, and packaging to obtain the finished product.
Example 1A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
70 parts of corn flour, 55 parts of feather protein powder, 20 parts of prebiotics, 15 parts of compound vitamin, 15 parts of amino acid, 30 parts of bone meal, 20 parts of calcium carbonate, 30 parts of bracken, 5 parts of salt, 25 parts of additive and 20 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: removing dust from the waste feather, rinsing, drying, puffing in a puffing machine at 250 deg.C under 15Mpa for 1.5 hr, sterilizing, dehydrating, deodorizing, and pulverizing to 20 mesh.
The prebiotics in the composition containing the feather albumen powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 18:7: 13.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 5:11:10:3:15 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 25:16: 21.
Example 2A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.2h at the temperature of 230 ℃ and the pressure of 13Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather-containing material.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 17:6: 12.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 23:15: 20.
Example 3A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
50 parts of corn flour, 35 parts of feather protein powder, 10 parts of prebiotics, 5 parts of compound vitamin, 5 parts of amino acid, 20 parts of bone meal, 10 parts of calcium carbonate, 15 parts of bracken, 1 part of salt, 15 parts of additive and 10 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: removing dust from waste feather, rinsing, drying, puffing in puffing machine at 200 deg.C and 10Mpa for 1.0h, sterilizing, dehydrating, deodorizing, and pulverizing to 20 mesh.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 15:5: 11.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 3:9:7:1:12 in mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 22:13: 19.
Comparative example 1A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 17:6: 12.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 23:15: 20.
The comparative example was prepared similar to example 2 for the composition containing feather protein powder.
The comparative example differs from example 2 in that: the feather protein powder in the comparative example is not puffed by a bulking machine.
Comparative example 2A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.2h at the temperature of 230 ℃ and the pressure of 13Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather-containing material.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 1:1: 1.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 23:15: 20.
The comparative example was prepared similar to example 2 for the composition containing feather protein powder.
The comparative example differs from example 2 in that: in the comparative example, the prebiotics consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 1:1: 1.
Comparative example 3A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.2h at the temperature of 230 ℃ and the pressure of 13Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather-containing material.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 17:6: 12.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 23:15: 20.
The comparative example was prepared similar to example 2 for the composition containing feather protein powder.
The comparative example differs from example 2 in that: no bone meal was added to this comparative example.
Comparative example 4A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 2 parts of salt, 19 parts of additive and 15 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.2h at the temperature of 230 ℃ and the pressure of 13Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather-containing material.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 17:6: 12.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of ferulic acid, resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 23:15: 20.
The comparative example was prepared similar to example 2 for the composition containing feather protein powder.
The comparative example differs from example 2 in that: no bracken was added to this comparative example.
Comparative example 5A composition containing feather protein powder
The composition containing the feather protein powder comprises the following components in parts by weight:
62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
The preparation method of the feather protein powder in the composition containing the feather protein powder comprises the following steps: dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.2h at the temperature of 230 ℃ and the pressure of 13Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather-containing material.
The prebiotics in the composition containing the feather protein powder consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 17:6: 12.
The compound vitamin in the composition containing feather protein powder comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is prepared from 4:10:9:2:13 by mass ratio.
The additive in the composition containing the feather protein powder consists of resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 15: 20.
The comparative example was prepared similar to example 2 for the composition containing feather protein powder.
The comparative example differs from example 2 in that: the additive in the comparative example consists of resistant dextrin and electrolytic multi-dimensional powder according to the mass ratio of 15: 20.
Test example one, growth promotion test of composition
Test samples: the feather protein powder-containing compositions prepared in examples 1 to 3 and comparative examples 1 to 5;
the test method comprises the following steps: 180 SPF pure male SD rats of 226 + -26 g weight were purchased from Shandong Lu anti-medicine GmbH at 3 weeks of age. After the rats are purchased, the rats are adapted to the environment for one week, water and diet are freely taken, the temperature of a feeding room is 25 ℃, the humidity is 60%, and the rats are alternately used day and night for 12 hours. Rats were divided into 9 groups of 20 rats each, and fed with test specimen samples and purified water, respectively.
And (3) weight detection: weighing the weight of each group of rats before feeding and after feeding for 6d and 10d respectively, recording the average value of the weight of each group of rats, and calculating the weight gain of 6d and 10 d;
bone growth test: feeding test samples, wherein the feeding amount is 100 g/time, injecting fluorescent matrix-tetracycline into the abdominal cavity after 6 days, injecting the fluorescent matrix-tetracycline for the 2 nd time on the 8 th day, intoxicating the 10 th rhizoma gastrodiae with rats to kill, completely stripping off the tibia, measuring the distance between the proximal articular surface and the distal articular surface of the tibia by using a vernier caliper, namely the length of the tibia fed for 10 days, and taking the average value of 3 times of measurement. In addition, the length of the tibia was measured after injection of the fluorescent matrix tetracycline at 56d and 60d, respectively, and 60 d. Tibial elongation is measured by taking the removed tibia as a bone slice, immunofluorescent staining the tetracycline, and measuring the distance between the fluorescent bands and the growth plate for tibial elongation between 2 d.
Rat bone densitometry test: a weaning Wistar male rat with SPF level born for about 4 weeks is selected, the weight of which is 60-75 g, and purchased from Beijing Huafukang Biotechnology GmbH. After the rats are purchased, the rats are adapted to the environment for one week, water and diet are freely taken, the temperature of a feeding room is 25 ℃, the humidity is 60%, and the rats are alternately used day and night for 12 hours. Rats were randomly divided into ten groups of 20 rats per group by weight, and the rats were fed test samples with a feeding amount of 100 g/time, sacrificed after 3 months, the femurs were dissected out, and the bone densities of the mid-point of the left femur and the distal end of the femur were measured using a peripheral dual-energy X-ray bone densitometer.
And (3) test results: the test results are shown in tables 1, 2 and 3.
Table 1 rat body weight test results
TABLE 2 rat bone growth test results
| Group of | Tibia growth length/mum of rat after 10d | Tibia growth length/mum of rat after 60d |
| Example 1 | 89 | 406 |
| Example 2 | 93 | 412 |
| Example 3 | 91 | 410 |
| Comparative example 1 | 85 | 387 |
| Comparative example 2 | 92 | 409 |
| Comparative example 3 | 67 | 365 |
| Comparative example 4 | 88 | 387 |
| Comparative example 5 | 91 | 410 |
| Purified water | 63 | 342 |
TABLE 3 rat bone Density test results
As can be seen from Table 1, the weight gains of the rats of 10d and 60d containing the feather albumen powder provided by the invention are obvious, the weight gain of 10d is 59-64g, and the weight gain of 60d is 374-382 g. As can be seen from Table 2, after the rats eat the compositions containing the feather albumen powder for 10 days and 60 days, the growth length of the shin bones of the rats is obvious, which fully indicates that the compositions containing the feather albumen powder can effectively promote the growth of the animals. As can be seen from Table 3, after feeding the composition containing feather protein powder provided by the invention for 3 months, the femoral midpoint density and the distal bone density of rats are both significantly improved. As can be seen from tables 1, 2 and 3, the weight gain of the rats fed with the composition containing feather protein powder prepared in example 3 was the greatest, the tibia grew the fastest and the bone density was the highest, so example 3 is the best example of the present invention.
Compared with example 3, comparative examples 1-5 respectively change the puffing of feather albumen powder, the dosage ratio of prebiotics, the dosage of bone meal, the dosage of fiddlehead and the proportion of additives, but the weight gain and the density growth of tibia and bone of rats are reduced to different degrees, which shows that the formula of the invention is optimized, and the effect is reduced by changing the formula.
Test example two, composition disease resistance test
Test samples: compositions prepared in examples 1 to 3 and comparative examples 4 to 5;
the test method comprises the following steps: 50 piglets aged 30 days are selected and randomly divided into 5 groups, each group comprises 10 piglets, the compositions prepared in the examples 1-3 and the comparative examples 4-5 are respectively fed according to the total-period amount of 31-40 days and 1kg of 41-50 days, and after the piglets are fed for 20 days, the disease condition and the death condition of the piglets are detected. In this process, piglets were managed in the same way;
and (3) test results: the test results are shown in Table 4.
TABLE 4 disease resistance Effect of the compositions
| Group of | Number of diarrhea/body | Death number/body |
| Example 1 | 1 | 0 |
| Example 2 | 0 | 0 |
| Example 3 | 0 | 1 |
| Comparative example 4 | 2 | 1 |
| Comparative example 5 | 3 | 2 |
As can be seen from Table 4, the composition containing feather protein powder provided by the invention can effectively reduce the morbidity of animals, wherein the composition prepared in example 3 has the best effect and is the best example of the invention. Compared with the embodiment 3, the comparative example 4 has no added bracken, but the diarrhea number and death number of the piglets are increased, which shows that the bracken contained in the bracken has a certain inhibition effect on pathogenic bacteria, and can reduce the disease incidence of animals; comparative example 5 does not add ferulic acid, but the diarrhea number and death number of piglets are increased, which shows that ferulic acid has physiological effects of scavenging free radicals, improving immunity, resisting bacteria, diminishing inflammation, reducing blood fat, reducing stress and the like, can be absorbed and eliminated by organisms, and can effectively reduce the illness rate of animals.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art will recognize that changes may be made to the embodiments described above without departing from the spirit and scope of the invention. Therefore, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the technical spirit of the present invention are covered by the claims of the present invention.
Claims (6)
1. The composition containing feather protein powder is characterized by comprising the following components in parts by weight:
50-70 parts of corn flour, 35-55 parts of feather protein powder, 10-20 parts of prebiotics, 5-15 parts of multivitamins, 5-15 parts of amino acid, 20-30 parts of bone meal, 10-20 parts of calcium carbonate, 15-30 parts of bracken, 1-5 parts of salt, 15-25 parts of additives and 10-20 parts of water;
the prebiotics consist of lactococcus lactis, yeast beta-glucan and bifidobacterium breve in a mass ratio of 15-18:5-7: 11-13; the additive consists of ferulic acid, resistant dextrin and electrolytic multi-vitamin powder in a mass ratio of 22-25:13-16: 19-21.
2. The composition containing feather protein powder as claimed in claim 1, which is characterized by comprising the following components in parts by weight: 62 parts of corn flour, 41 parts of feather protein powder, 15 parts of prebiotics, 11 parts of compound vitamin, 12 parts of amino acid, 27 parts of bone meal, 16 parts of calcium carbonate, 27 parts of bracken, 2 parts of salt, 19 parts of additive and 15 parts of water.
3. The composition containing the feather protein powder as claimed in claim 1, wherein the preparation method of the feather protein powder comprises the following steps: the method comprises the steps of dedusting, rinsing and drying the waste feather, then putting the waste feather into a bulking machine, bulking for 1.0-1.5h at the temperature of 200-250 ℃ and under the pressure of 10-15Mpa, then sterilizing, dehydrating and deodorizing, and finally crushing the deodorized feather to 20 meshes to obtain the feather powder.
4. The composition containing feather protein powder of claim 1, wherein the vitamin complex comprises vitamin A and vitamin B2Vitamin B6Vitamin B12And the vitamin D is composed of 3-5:9-11:7-10:1-3:12-15 by mass ratio.
5. The composition containing feather protein powder as claimed in claim 1, wherein the amino acid consists of lysine, methionine, tryptophan, histidine, isoleucine, leucine, phenylalanine, valine, threonine and arginine in a mass ratio of 3:1:5:4:9:7:2:6:11: 8.
6. A method for preparing the composition containing the feather protein powder according to any one of claims 1 to 5, which is characterized by comprising the following steps:
s1, uniformly mixing corn flour, feather protein powder, prebiotics, compound vitamins, bone meal and calcium carbonate to obtain a mixture;
s2, putting the fiddlehead into a grinder, adding water, and grinding into slurry;
s3, mixing the mixture obtained in the step S1 with the slurry obtained in the step S2, adding amino acid, salt and additives, uniformly stirring, introducing into a granulator with the caliber of a ring die of 5.0mm, and carrying out extrusion granulation to obtain granules;
s4, drying the granules prepared in the step S3, cooling, sieving to remove powder, and packaging to obtain the finished product.
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| CN103704469A (en) * | 2014-01-14 | 2014-04-09 | 夏歌骏 | Production process for puffed feather meal |
| CN105901289A (en) * | 2016-04-20 | 2016-08-31 | 李森泉 | Complete feed for pork pigs |
| CN110720557A (en) * | 2019-11-21 | 2020-01-24 | 安徽徽牛牧业有限公司 | Beef cattle feed and preparation process thereof |
| CN112004417A (en) * | 2018-04-25 | 2020-11-27 | 诺维信公司 | Animal feed composition and use thereof |
| CN112006179A (en) * | 2020-08-21 | 2020-12-01 | 安徽绿保源养殖有限公司 | Piglet feed |
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| CN103704469A (en) * | 2014-01-14 | 2014-04-09 | 夏歌骏 | Production process for puffed feather meal |
| CN105901289A (en) * | 2016-04-20 | 2016-08-31 | 李森泉 | Complete feed for pork pigs |
| CN112004417A (en) * | 2018-04-25 | 2020-11-27 | 诺维信公司 | Animal feed composition and use thereof |
| CN110720557A (en) * | 2019-11-21 | 2020-01-24 | 安徽徽牛牧业有限公司 | Beef cattle feed and preparation process thereof |
| CN112006179A (en) * | 2020-08-21 | 2020-12-01 | 安徽绿保源养殖有限公司 | Piglet feed |
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