KR102376381B1 - Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used - Google Patents

Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used Download PDF

Info

Publication number
KR102376381B1
KR102376381B1 KR1020177018724A KR20177018724A KR102376381B1 KR 102376381 B1 KR102376381 B1 KR 102376381B1 KR 1020177018724 A KR1020177018724 A KR 1020177018724A KR 20177018724 A KR20177018724 A KR 20177018724A KR 102376381 B1 KR102376381 B1 KR 102376381B1
Authority
KR
South Korea
Prior art keywords
livestock
bacteria
acid
agent
feed
Prior art date
Application number
KR1020177018724A
Other languages
Korean (ko)
Other versions
KR20170117382A (en
Inventor
유타카 야마다
히로키 마쓰이
Original Assignee
유가산교 가부시키가이샤
고쿠리츠다이가쿠호진 미에다이가쿠
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 유가산교 가부시키가이샤, 고쿠리츠다이가쿠호진 미에다이가쿠 filed Critical 유가산교 가부시키가이샤
Publication of KR20170117382A publication Critical patent/KR20170117382A/en
Application granted granted Critical
Publication of KR102376381B1 publication Critical patent/KR102376381B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Polymers & Plastics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Fodder In General (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

본 발명은, 가축 등의 동물의 장내 환경을 개선시켜, 이에 따라 질병의 발생을 미연에 방지하여, 가축의 생산성을 향상시키는 것을 목적으로 한다.
본 발명의 동물장내 유용균 증가제는, 카프릴산, 카프르산 및 라우르산으로 이루어지는 군으로부터 선택되는 적어도 1종류의 지방산 또는 그 염을 함유한다.An object of the present invention is to improve the intestinal environment of animals such as livestock, thereby prevent the occurrence of diseases in advance, and improve the productivity of livestock.
The beneficial bacteria increasing agent in the animal intestine of the present invention contains at least one fatty acid or a salt thereof selected from the group consisting of caprylic acid, capric acid and lauric acid.

Description

동물장내 유용균 증가제 및 이것을 사용한 가축장내 환경개선방법{AGENT FOR INCREASING USEFUL BACTERIA IN ANIMAL INTESTINES, AND METHOD FOR IMPROVING INTESTINAL ENVIRONMENT IN LIVESTOCK IN WHICH SAID AGENT IS USED}An agent for increasing useful bacteria in the animal intestine and a method for improving the environment in the livestock field using the same

본 발명은 동물의 장내(腸內)에 존재하는 유용균(有用菌)을 증가시키는 동물장내 유용균 증가제에 관한 것이다. 또한 본 발명은, 본 발명의 동물장내 유용균 증가제를 사용하여 가축이 구비하는 장내 세균총(腸內 細菌叢) 중의 유용균을 증가시키고 또한 유해균을 감소시킴으로써 가축의 장내 환경을 개선시켜서, 그에 따라 소화생리기능이나 면역기능의 개선과 유지를 도모하고, 질병의 발생을 미연에 방지하여, 가축의 효율적 생산성을 개선시키는 가축장내 환경개선방법에도 관한 것이다.The present invention relates to an agent for increasing useful bacteria in the intestinal tract of animals. In addition, the present invention improves the intestinal environment of livestock by increasing useful bacteria in the intestinal flora of livestock and reducing harmful bacteria by using the beneficial bacteria increasing agent in the animal intestine of the present invention, thereby improving digestive physiology It also relates to a method for improving the environment in a livestock farm to improve and maintain functions or immune functions, to prevent the occurrence of diseases in advance, and to improve the efficient productivity of livestock.

본 발명에 있어서의 「동물」이란, 포유류, 조류, 파충류, 양서류, 어류 등의 척추동물로서, 예를 들면 인간, 가축, 영장류(원숭이, 침팬지 등), 실험동물(마우스, 래트 등) 등이다. 또한 본 발명에 있어서의 「가축」이란, 사람이 그 생산물(젖(乳), 고기(肉), 알(卵) 등)을 이용하기 위해서 순조롭게 도달하거나 내지는 사육하고 있는 산업동물, 펫(pet)이나 감상용의 애완동물, 경기용(競技用) 동물이며, 예를 들면 소, 돼지, 면양, 염소, 말, 가금(家禽), 개, 고양이, 작은 새, 양어(養魚) 등이 포함된다.
In the present invention, the term "animal" refers to vertebrates such as mammals, birds, reptiles, amphibians, and fish, such as humans, livestock, primates (monkeys, chimpanzees, etc.), experimental animals (mouses, rats, etc.). . In addition, "livestock" in the present invention refers to industrial animals, pets, that humans reach or breed smoothly in order to use their products (milk, meat, eggs, etc.) It is a pet for appreciation or competition, and includes, for example, cattle, pigs, sheep, goats, horses, poultry, dogs, cats, small birds, and fish.

최근, 가축은 대규모 사육형태가 한층더 진행되어, 개체가 아니라 군관리(群管理)가 부득이하게 이루어지고 있다. 그 현상(現狀)에 있어서 높은 사육밀도(동물 또는 새/면적)에 의한 단기간사육이나, 영양이나 형태가 다른 사료로의 변경, 서열(暑熱), 한냉, 이유(離乳), 사육장에서의 사람 본위(本位)에 의한 군이동(群移動), 가축의 장거리수송 등의 여러가지 관리 스트레스에 노출되고 있다. 특히 하계(夏季)에는, 지구온난화에 의하여 지금까지 없었던 기온상승에 의해 가축이 받는 스트레스는 현저하게 증대하고, 한편으로는 한냉기(寒冷期)에 있어서의 스트레스에 대해서도 변함이 없어, 이들 요인이 가축에 주는 마이너스의 영향은 늘어나기만 한다. 그 결과, 질병의 발생이나 성장(증체(增體))의 저하 등의 생산성 저하, 및 생산물의 품질에 대한 영향이 큰 문제가 되고 있다.In recent years, large-scale breeding of livestock has progressed further, and group management, not individual, is inevitably made. Short-term breeding due to high breeding density (animal or bird/area) in the present situation, change to feed with different nutrition or form, sequence, cold, weaning, human standard in breeding grounds They are exposed to various management stresses such as military movement due to (本位) and long-distance transport of livestock. In particular, in the summer, the stress that livestock receives due to the unprecedented increase in temperature due to global warming increases remarkably, and on the other hand, the stress in the cold season does not change either, and these factors are The negative impact on livestock only increases. As a result, the decrease in productivity, such as the occurrence of diseases and the decrease in growth (increased body), and the influence on the quality of products are a major problem.

스트레스가 가축의 생산성을 저하시키는 간접적 요인으로서, 소화관 등의 내장이 허약화하여, 사료섭취량의 저하 또는 영양성분의 소화와 흡수의 현저한 저하, 장내 세균총의 교란이나 면역력의 저하에 의하여, 세균이나 원충(原蟲) 등의 병원체에 감염되기 쉬워지는 것을 들 수 있다. 장내 세균총에는 유용균과 유해균이 포함되고, 유용균으로서 Lactobacillus속(屬)이나 Bifidobacterium속에 속하는 균 등을 들 수 있고, 한편 유해균으로서 Escherichia Coli, Salmonella속에 속하는 균, Campylobacter jejuni, Clostridium perfingers 등의 균을 들 수 있다. 이들 유용균과 유해균과의 경쟁적인 증식의 균형이 무너져서 유해균이 우세한 장내 세균총이 됨으로써 면역기능의 저하나 소화생리기능의 저하를 야기하여, 결과적으로 질병의 발생이나 생산성의 저하로 이어진다고 생각되고 있다.As an indirect factor that reduces the productivity of livestock, stress is an indirect factor that weakens the intestines such as the digestive tract, causing a decrease in feed intake or a significant decrease in digestion and absorption of nutrients, disturbance of the intestinal flora or a decrease in immunity, resulting in bacteria or protozoa (origin) which becomes easy to be infected with pathogens, such as, is mentioned. The intestinal flora includes useful bacteria and harmful bacteria, and useful bacteria include bacteria belonging to the genus Lactobacillus and Bifidobacterium, and bacteria belonging to the genera Escherichia Coli and Salmonella, Campylobacter jejuni, Clostridium perfingers, etc. can be mentioned as harmful bacteria. there is. It is thought that the balance of the competitive growth of these useful and harmful bacteria is disrupted, and harmful bacteria become the dominant intestinal flora, resulting in a decrease in immune function or a decrease in digestive and physiological functions, which in turn leads to the occurrence of diseases and a decrease in productivity.

스트레스를 받은 가축에 대한 대처법으로서, 예를 들면 고급지방산 트리글리세리드와 중쇄지방산 트리글리세리드를 함유하는 가축용 영양보급제(특허문헌1), 동식물성 유지와 식물섬유를 함유하고 유축(幼畜)의 설사·무른 대변의 개선 및 스트레스의 경감을 목적으로 하는 유축용 대용유 조성물(특허문헌2), 주성분으로서 탈지분유 및/또는 대두밀(soybean meal)을 포함하고 또한 중쇄지방산 또는 그 염을 함유하는 자축용 대용유 조성물(仔畜用 代用乳 組成物)(특허문헌3), 중쇄지방산 혼합물을 포함하고 대장균에 의한 오염 등을 저지하기 위한 의약(특허문헌4) 등이 보고되어 있다.
As a coping method for stressed livestock, for example, a nutritional supplement for livestock containing high-fatty acid triglycerides and medium-chain fatty acid triglycerides (Patent Document 1), containing animal and vegetable oils and fats and plant fiber, and diarrhea and A substitute oil composition for breast milk (Patent Document 2) for the purpose of improving soft stool and alleviating stress; Compositions (仔畜用大用乳組成物) (Patent Document 3), including a medium-chain fatty acid mixture, drugs for preventing contamination by E. coli, etc. (Patent Document 4), etc. have been reported.

일본국 공개특허 특개평6-153812호 공보Japanese Patent Laid-Open No. 6-153812 일본국 공개특허 특개평10-127232호 공보Japanese Patent Laid-Open No. 10-127232 일본국 특허 제2723961호 공보Japanese Patent No. 2723961 Publication 일본국 특허 제5259905호 공보Japanese Patent No. 5259905 Publication

그러나 고급지방산 트리글리세리드와 중쇄지방산 트리글리세리드의 보급만으로, 또는 식물섬유와의 병용만으로는 가축의 활력이 향상될 때까지 시간을 요하기 때문에, 병원체가 감염되는 시간적 여유를 생겨 버려, 생산성 저하의 개선이 곤란해져버린다. 게다가 영양적, 사육관리적 및 사육환경적 스트레스에 의하여 발생하는 생체조정기능이나 면역기능의 저하를 개선하고, 또한 장관점막(腸管粘膜), 특히 융모(絨毛) 등을 수복하지 않으면, 충분한 성장, 사료효율 등의 생산성의 개선이나 설사 등의 질병발생의 개선은 바랄 수 없는 등의 문제점이 있다. 또한 중쇄지방산의 혼합물에 관해서도 영양보급효과나 장내 대장균의 억제효과는 있지만, 다른 유해균의 억제나 비피더스균 등의 유용균에 주는 영향에 대해서는 알려져 있지 않다.However, since it takes time until the vitality of livestock is improved only by supplying higher fatty acid triglycerides and medium-chain fatty acid triglycerides or only by using dietary fiber in combination, there is time to infect pathogens, making it difficult to improve productivity. throw it away In addition, if nutritional, breeding management and breeding environmental stress does not improve the degradation of the biological control function or immune function, and the intestinal mucosa, especially the villi, etc. are not repaired, sufficient growth and feed There are problems in that productivity such as efficiency cannot be improved or disease outbreaks such as diarrhea can be improved. In addition, the mixture of medium-chain fatty acids also has a nutritional supplementation effect and an inhibitory effect on E. coli in the intestine, but it is not known about the effect on the inhibition of other harmful bacteria or useful bacteria such as Bifidobacterium.

본 발명은, 상기한 일련의 사항에 착안하여, 가축 등의 동물의 장내 환경을 개선시켜, 그에 따라 질병의 발생을 미연에 막아, 가축의 생산성을 향상시키는 것을 목적으로 한다.
An object of the present invention is to improve the intestinal environment of animals such as livestock by paying attention to the above series of matters, thereby preventing the occurrence of diseases in advance, and improving the productivity of livestock.

본 발명자들은, 상기 과제를 해결하기 위해서 예의 검토를 한 결과, 특정한 산 또는 그 염이 가축 등의 동물이 구비하는 장내 세균총내에 유용균을 증가시키고 또한 유해균을 저하시킴으로써 장내 환경을 개선시켜, 질병의 발생이나 생산성 저하를 방지할 수 있는 것을 찾아내어, 본 발명을 완성시키기에 이르렀다.As a result of intensive studies to solve the above problems, the present inventors have improved the intestinal environment by increasing useful bacteria in the intestinal flora of animals such as livestock and lowering harmful bacteria, whereby a specific acid or salt thereof improves the intestinal environment, resulting in disease However, it was found that a decrease in productivity could be prevented, and the present invention was completed.

즉 본 발명은, 카프릴산(caprylic acid), 카프르산(capric acid) 및 라우르산(lauric acid)으로 이루어지는 군으로부터 선택되는 적어도 1종류의 지방산 또는 그 염을 함유하는 동물장내 유용균 증가제이다.That is, the present invention provides an agent for increasing useful bacteria in the intestinal tract containing at least one fatty acid or a salt thereof selected from the group consisting of caprylic acid, capric acid and lauric acid. am.

또 본 발명은, 본 발명의 동물장내 유용균 증가제가 0.1∼2중량％ 배합된 사료를 가축에게 급여하는 것을 특징으로 하는 가축장내 환경개선방법이다.
In addition, the present invention is a method for improving the environment in a livestock field, characterized in that the feed containing 0.1 to 2% by weight of the beneficial bacteria increasing agent in the animal intestine of the present invention is fed to livestock.

동물의 장관(腸管)내에는 다수의 세균군이 존재해 장내 세균총을 형성하고 있다. 본 발명의 동물장내 유용균 증가제는, 가축 등의 동물이 구비하는 장내 세균총내에 유용균을 증가시키고 또한 유해균을 저하시켜서, 동물의 장내 환경을 유용균이 우세한 세균총으로 개선하고 질병의 발생을 미연에 방지하여, 생산성을 향상시킬 수 있다. 게다가 주성분인 카프릴산, 카프르산 및 라우르산은, 원래 흡수속도와 대사속도가 극히 빠른 물질로, 섭취함으로써 활력이 빠르게 얻어진다고 하는 점에서도 유효하다.A large number of bacterial groups exist in the intestinal tract of animals to form the intestinal flora. The useful bacteria increasing agent in the animal intestine of the present invention increases useful bacteria in the intestinal flora of animals such as livestock and lowers harmful bacteria, improves the intestinal environment of animals to a bacterial flora in which useful bacteria dominate, and prevents the occurrence of diseases in advance. , can improve productivity. In addition, caprylic acid, capric acid, and lauric acid, which are the main components, are substances with extremely fast absorption and metabolism rates, and are effective in terms of rapidly gaining vitality by ingesting them.

또한 본 발명의 가축장내 환경개선방법은, 가축의 장내 환경을 개선하고 질병의 발생을 미연에 방지하여 생산성을 향상시킬 수 있다.
In addition, the method for improving the intestinal environment of the present invention can improve the intestinal environment of livestock and prevent the occurrence of diseases in advance, thereby improving productivity.

이하, 본 발명에 대하여 상세하게 설명한다.Hereinafter, the present invention will be described in detail.

본 발명의 동물장내 유용균 증가제(이하, 간단하게 「유용균 증가제」라고도 한다)는, 카프릴산(caprylic acid), 카프르산(capric acid) 및 라우르산(lauric acid)으로 이루어지는 군으로부터 선택되는 적어도 1종류의 지방산 또는 그 염(이하, 「지방산 등」이라고도 한다)을 함유한다. 카프릴산, 카프르산 및 라우르산으로 이루어지는 군으로부터 선택되는 2종류의 지방산또는 염을 함유하는 것이 바람직하고, 이들 3종류의 지방산 또는 염을 함유하는 것이 특히 바람직하다.The useful bacteria increasing agent in the animal intestine of the present invention (hereinafter also simply referred to as "effective bacteria increasing agent") is from the group consisting of caprylic acid, capric acid and lauric acid. At least one selected fatty acid or a salt thereof (hereinafter, also referred to as "fatty acid etc.") is contained. It is preferable to contain two types of fatty acids or salts selected from the group which consists of caprylic acid, capric acid, and lauric acid, and it is especially preferable to contain these three types of fatty acids or salts.

상기 지방산의 염으로서는, 가축에 대한 필수 미네랄 성분인 점으로부터, 상기한 각종 지방산의 칼슘염 및 마그네슘염이 적합하고, 이들은 1종으로 사용해도 좋고, 2종을 조합시켜서 사용해도 좋다. 이들 지방산의 금속염은 상온에서는 고체로서, 취급이 용이하고 또 사료에 혼화(混和)하기 쉽다는 점에서, 또한 반추동물(反芻動物)의 루멘(lumen)에 대한 영향을 고려하여, 지질중에서는 이들 지방산 금속염이 바람직하다. 특히 고체분말상(固體粉末狀) 혹은 과립상(顆粒狀)의 지방산 칼슘이나 지방산 마그네슘을 사용하는 것이 더 바람직하다.As the salt of fatty acid, calcium salt and magnesium salt of various fatty acids described above are suitable from the viewpoint of being an essential mineral component for livestock, and these salts may be used alone or in combination of two kinds. Metal salts of these fatty acids are solid at room temperature, easy to handle, and easy to mix with feed. Fatty acid metal salts are preferred. In particular, it is more preferable to use calcium fatty acid or magnesium fatty acid in solid powder form or granular form.

본 발명의 유용균 증가제는, 소, 돼지, 닭 등의 가축에 적용할 수 있고, 예를 들면 높은 사육밀도(동물 또는 새/면적)에 의한 단기간사육이나, 영양이나 형태가 다른 사료로의 변경, 서열, 한냉, 이유, 사육장에서의 사람 본위에 의한 군이동, 가축의 장거리수송 등의 여러가지 관리 스트레스를 받는 시기에 있어서, 장내의 유해균을 감소시키고 유용균을 증가시킴으로써 질병을 미연에 방지하고 사료섭취량의 유지나 성장을 촉진시켜 생산성을 유지할 수 있다.The useful bacteria increasing agent of the present invention can be applied to livestock such as cattle, pigs, chickens, etc., for example, short-term breeding due to high breeding density (animal or bird/area), or change to feed with different nutrition or shape In times of various management stresses, such as sequencing, cold, weaning, movement by humans in the kennel, and long-distance transport of livestock, it reduces harmful bacteria in the intestine and increases useful bacteria to prevent diseases and feed intake It is possible to maintain productivity by promoting the maintenance or growth of

본 발명의 유용균 증가제가 배합된 가축용 사료(이하, 본 발명의 가축용 사료라고도 한다)의 급여에 의하여 감소시킬 수 있는 유해균은, 예를 들면Escherichia coli, Salmonella속, Clostridium perfingers 등을 들 수 있고, 특히 Escherichia coli, Clostridium perfingers에 대하여 강한 효과를 나타낸다. 또 본 발명의 가축용 사료의 급여에 의하여 증가시킬 수 있는 유용균은, 예를 들면 Lactobacillus속이나 Bifidobacterium속에 속하는 균 등을 들 수 있다.Harmful bacteria that can be reduced by feeding the feed for livestock containing the useful bacteria increasing agent of the present invention (hereinafter also referred to as the feed for livestock of the present invention) include, for example, Escherichia coli, Salmonella genus, Clostridium perfingers, etc. , especially against Escherichia coli and Clostridium perfingers. In addition, useful bacteria that can be increased by feeding the feed for livestock of the present invention include, for example, bacteria belonging to the genus Lactobacillus and the genus Bifidobacterium.

본 발명의 유용균 증가제는, 담체(擔體), 부형제(賦形劑) 등의 공지의 첨가제를 함유하고 있어도 좋다. 본 발명의 유용균 증가제에 있어서 상기한 지방산 등의 함유량은, 바람직하게는 70중량％ 이상, 특히 바람직하게는 90중량％ 이상, 더 바람직하게는 95중량％ 이상이다. 함유량이 70중량％미만에서는, 동물의 장내 세균총을 개선하기 위해서는 불충분해지는 경우가 있고, 또 장쇄지방산이나 미네랄 등의 타성분도 급여하게 되어, 비효율적이다.The useful bacteria increasing agent of this invention may contain well-known additives, such as a carrier and an excipient. In the useful bacteria increasing agent of the present invention, the content of the above fatty acids and the like is preferably 70% by weight or more, particularly preferably 90% by weight or more, and more preferably 95% by weight or more. If the content is less than 70% by weight, it may become insufficient to improve the intestinal flora of animals, and other components such as long-chain fatty acids and minerals are also supplied, which is inefficient.

본 발명의 유용균 증가제 및 가축용 사료는, 식품첨가제, 의약품으로서 사용할 수 있고, 또 가축용 사료의 첨가제로서도 사용할 수 있다.The useful bacteria increasing agent and feed for livestock of the present invention can be used as food additives and pharmaceuticals, and can also be used as an additive for feed for livestock.

가축용 사료는, 예를 들면 옥수수분, 쌀가루, 겨 등의 곡분(穀粉), 무기물, 아미노산, 단백질, 비타민류, 지질 등을 포함하고 있어도 좋다. 예를 들면 일본표준사료성분표(2009년판, 독립행정법인 농업·식품산업기술종합연구기구(National Agriculture and Food Research Organization) 편찬)에 기재된 성분을 포함하고 있어도 좋다.The feed for livestock may contain, for example, cornmeal, rice flour, wheat flour such as bran, inorganic substances, amino acids, proteins, vitamins, lipids, and the like. For example, the ingredients described in the Japanese Standard Feed Ingredients Table (2009 edition, compiled by the National Agriculture and Food Research Organization, an independent administrative corporation) may be included.

본 발명의 유용균 증가제 및 가축용 사료의 형태로서는, 액상(液狀), 페이스트상(paste狀), 분말상(粉末狀), 입상(粒狀), 펠렛(pellet) 등 어느 형태여도 좋고, 원하는 형태에 따라 공지의 방법으로 조제할 수 있다.As the form of the useful bacteria increasing agent and livestock feed of the present invention, any form such as liquid, paste, powder, granular, pellet, etc. may be used. Depending on the form, it can be prepared by a known method.

본 발명의 가축장내 환경개선방법에 있어서는, 본 발명의 유용균 증가제가 0.1∼2중량％, 바람직하게는 0.10∼1.5중량％, 특히 바람직하게는 0.5∼1중량％ 배합된 사료를 가축에 급여하는 것을 특징으로 한다. 가축에 대한 급여량이 사료중 0.1중량％ 미만에서는, 본 발명의 효과가 얻어지지 않는 경우가 있다.In the method for improving the environment in the livestock field of the present invention, feeding the livestock a feed formulated with the useful bacteria increasing agent of the present invention 0.1 to 2% by weight, preferably 0.10 to 1.5% by weight, particularly preferably 0.5 to 1% by weight characterized. If the feed amount to livestock is less than 0.1% by weight in the feed, the effect of the present invention may not be obtained.

또 본 발명의 유용균 증가제가 배합된 사료의 급여빈도나 급여량은, 사료의 물성(物性), 가축의 종류나 체중(연령) 등에 따라 다르고, 당해 사료의 통상의 급여빈도나 급여량을 채용할 수 있다.In addition, the feeding frequency and feeding amount of the feed containing the useful bacteria increasing agent of the present invention varies depending on the physical properties of the feed, the type or weight (age) of the livestock, etc., and the normal feeding frequency and feeding amount of the feed can be adopted. .

실시예Example

이하, 실시예에 의하여 본 발명을 더 상세하게 설명하지만, 본 발명은 그 요지를 넘지 않는 한 이들에 한정되지 않는다.Hereinafter, although an Example demonstrates this invention in more detail, this invention is not limited to these unless the summary is exceeded.

실시예에 사용하는 가축용 사료로서, 카프릴산Ｃａ, 카프르산Ｃａ, 라우르산Ｃａ, 카프릴산·카프르산·라우르산 혼합 지방산Ｃａ의 4종류의 가축용 사료를 준비했다. 또 「카프릴산·카프르산·라우르산 혼합 지방산Ｃａ」은, 카프릴산 : 카프르산 : 라우르산 = 20 : 20 : 60(중량비)으로 이루어지는 혼합 지방산의 칼슘염으로, 유화산업주식회사(YUKA SANGYO CO., LTD.) 제품의 상품이다.As feed for livestock used in Examples, four types of feed for livestock were prepared: caprylic acid Cc, capric acid Cc, lauric acid Cc, and caprylic/capric/lauric acid mixed fatty acid Cc. In addition, "caprylic acid, capric acid, lauric acid mixed fatty acid Cc" is a calcium salt of a mixed fatty acid composed of caprylic acid: capric acid: lauric acid = 20: 20: 60 (weight ratio), and is used in the emulsification industry. It is a product of YUKA SANGYO CO., LTD.

가축용 사료의 지방산 함유율을 표1에 나타낸다.Table 1 shows the fatty acid content of feed for livestock.

실시예에 사용한 가축용 사료의 지방산 함유율Fatty acid content of feed for livestock used in Examples 가축용 사료livestock feed 지방산 함유율（％）Fatty acid content (%) 카프릴산Ｃａcaprylic acid ７２．２７２．２ 카프르산Ｃａcapric acid ７５．６７５．６ 라우르산ＣａLauric acid C ７８．３７８．３ 카프릴산·카프르산·라우르산
혼합 지방산ＣａCaprylic acid, capric acid, lauric acid
mixed fatty acids ７７．３７７．３

〔실시예1∼5, 비교예1∼5〕[Examples 1 to 5, Comparative Examples 1 to 5]

물(水) 및 표2에 나타낸 기초사료를 포식(飽食), 급여하고 있는 홀스타인 착유소(Holstein 窄乳牛) 50마리를 실시예 및 비교예별로 5마리 사용하고, 표3 및 표4에 나타낸 가축용 사료를 3주(週)간 급여했다. 급여 3주 후에 분(糞)을 샘플링하고, 분석하기까지 동결(凍結) 보존했다. 분중의 세균수는, 리얼타임 ＰＣＲ법에 의하여 Bifidobacterium속, Escherichia coli, Salmonella속, Campylobacter jejumi., Clostridium perfringens에 대해서 조사했다. 구체적으로는, 1)검체(檢體)로부터 ＤＮＡ를 추출, 2)그 ＤＮＡ를 사용해서 각 ＰＣＲ법으로 분석, 3)유전자량과 세균수와의 비례관계에 의거하여 세균수를 정량하는 순서에 따라 세균수를 구하였다. 그리고 급여하기 전의 세균수를 1로 한 상대값으로서 산출했다. 또한 멀티플렉스 ＰＣＲ법에 의하여, 분변(糞便)중의 Lactobacillus속 세균 9종류, 즉 Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus reuteri의 검출을 하였다. 또한 급여 기간중의 설사 발생률, 급여 3주 후의 젖생산량, 유지율(乳脂率), 유단백질률을 조사했다. 각 실시예의 결과를 표5, 표7, 표9 및 표10에, 각 비교예의 결과를 표6, 표8, 표9 및 표10에 각각 나타낸다.50 Holstein dairy cows fed and fed with water and the basic feed shown in Table 2 were used in each of Examples and Comparative Examples, 5 animals were used, and the livestock shown in Tables 3 and 4 were used. For 3 weeks (週) was fed. Manure was sampled 3 weeks after feeding and stored frozen until analysis. Bifidobacterium genus, Escherichia coli, Salmonella genus, Campylobacter jejumi., and Clostridium perfringens were investigated for the number of bacteria in the manure by real-time PRC method. Specifically, 1) DNA is extracted from a sample, 2) DNA is used and analyzed by each PRC method, 3) the number of bacteria is quantified based on the proportional relationship between the amount of gene and the number of bacteria. The number of bacteria was calculated accordingly. Then, the number of bacteria before feeding was calculated as a relative value with 1. In addition, 9 types of bacteria of the genus Lactobacillus, namely, Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus plantarum, Lactobacillus rhamnosus, were detected by the multiplex PRC method. In addition, the incidence of diarrhea during the feeding period, the milk production 3 weeks after the feeding, the maintenance rate, and the milk protein rate were investigated. The results of each Example are shown in Tables 5, 7, 9 and 10, and the results of each comparative example are shown in Tables 6, 8, 9 and 10, respectively.

기초사료의 배합비율Basic feed mixing ratio 원료Raw material 배합비율（중량％）Blending ratio (% by weight) 소주 디스틸러스 솔류블
(distillers soluble)Soju Distillers Soluble
(distillers soluble) ３４．３34．3 간장박(soy sauce lees)soy sauce lees １５．１155.1 알팔파(Alfalfa)Alfalfa １５．５157.5 옥수수사일리지(corn silage)Corn silage １０．２10.2 블루그래스(Blueglass)Bluegrass ９．０9. 0 시판배합사료commercially available feed １５．０15. 0 비타민미네랄믹스vitamin and mineral mix ０．９0．9

기초사료에 대한 가축용 사료 첨가량Amount of feed for livestock to basic feed
실시예Example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 카프릴산Ｃａcaprylic acid 1.0%1.0% －- －- 0.2%0.2% －- 카프르산Ｃａcapric acid －- 0.5%0.5% －- －- －- 라우르산ＣａLauric acid C －- －- 0.1%0.1% 0.2%0.2% －- 카프릴산·카프르산·라우르산 혼합 지방산CaMixed fatty acid Ca of caprylic acid, capric acid, and lauric acid －- －- －- －- 0.5%0.5%


비교예comparative example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 카프릴산Ｃａcaprylic acid 0.05%0.05% －- －- －- －- 카프릴산·카프르산·라우르산 혼합 지방산CaMixed fatty acid Ca of caprylic acid, capric acid, and lauric acid －- 0.08%0.08% －- －- －- 팜유 지방산CaPalm Oil Fatty Acid Ca －- －- 1%One% －- －- 대두유 지방산CaSoybean Oil Fatty Acid Ca －- －- －- 2%2% －- 아마인유 지방산CaLinseed oil fatty acid Ca －- －- －- －- 1%One%



분중 균수 상대값The relative value of the number of germs in the minute 실시예Example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 Bifidobacterium속genus Bifidobacterium 1.151.15 1.071.07 1.021.02 1.071.07 1.181.18 Escherichia coliEscherichia coli 0.200.20 0.560.56 0.770.77 0.430.43 0.160.16 Salmonella속Salmonella 0.980.98 0.950.95 0.970.97 0.970.97 0.930.93 Campylobacter jejuniCampylobacter jejuni 1.151.15 1.141.14 1.161.16 1.161.16 1.131.13 Clostridium perfringensClostridium perfringens 0.740.74 0.830.83 0.890.89 0.800.80 0.700.70



분중 균수 상대값The relative value of the number of germs in the minute 비교예comparative example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 Bifidobacterium속genus Bifidobacterium 0.980.98 0.990.99 0.990.99 1.001.00 1.001.00 Escherichia coliEscherichia coli 1.011.01 1.031.03 1.071.07 0.990.99 1.021.02 Salmonella속Salmonella 1.061.06 1.041.04 1.101.10 1.011.01 1.051.05 Campylobacter jejuniCampylobacter jejuni 1.261.26 1.201.20 1.231.23 1.201.20 1.171.17 Clostridium perfringensClostridium perfringens 1.131.13 1.071.07 1.221.22 1.111.11 1.101.10



분중 Lactobacillus속 검출 마릿수／５마리Number of Lactobacillus genus detected per minute／5 실시예Example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 L. acidophilusL. acidophilus 00 00 00 00 00 L. casei-groupL. casei-group 22 22 1One 22 22 L. delbrueckiiL. delbrueckii 00 00 00 00 00 L. gasseriL. gasseri 33 33 22 33 33 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 00 00 00 00 00 L. reuteriL. reuteri 00 00 00 00 00



분중Lactobacillus속 검출 마릿수／５마리Number of animals detected in the genus Lactobacillus / 5 animals per minute 비교예comparative example No.1No.1 No.2No.2 No.3No.3 No.4No.4 No.5No.5 L. acidophilusL. acidophilus 00 00 00 00 00 L. casei-groupL. casei-group 00 00 00 00 00 L. delbrueckiiL. delbrueckii 00 00 00 00 00 L. gasseriL. gasseri 00 00 00 00 00 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 00 00 00 00 00 L. reuteriL. reuteri 00 00 00 00 00

설사발생률（%）Diarrhea incidence (%)

실시예

Example No.1No.1 0（0/5）0 (0/5) No.2No.2 0（0/5）0 (0/5) No.3No.3 20（1/5）20 (1/5) No.4No.4 0（0/5）0 (0/5) No.5No.5 0（0/5）0 (0/5)

비교예

comparative example No.1No.1 80（4/5）80 (4/5) No.2No.2 60（3/5）60 (3/5) No.3No.3 80（4/5）80 (4/5) No.4No.4 60（3/5）60 (3/5) No.5No.5 40（2/5）40 (2/5)

젖생산량（kg/일）Milk production (kg/day) 유지율(%)Retention (%) 유단백질률(%)Milk protein percentage (%)

실시예

Example No.1No.1 3939 3.63.6 3.13.1 No.2No.2 3838 3.63.6 3.13.1 No.3No.3 3737 3.73.7 3.23.2 No.4No.4 3838 3.63.6 3.13.1 No.5No.5 3939 3.63.6 3.13.1

비교예

comparative example No.1No.1 3333 3.73.7 3.13.1 No.2No.2 3434 3.63.6 3.13.1 No.3No.3 3434 3.63.6 3.13.1 No.4No.4 3434 3.63.6 3.13.1 No.5No.5 3535 3.63.6 3.13.1

표5에 나타나 있는 바와 같이, 본 발명에 관한 실시예1∼5의 유용균 증가제를 급여한 소의 분중의 Bifidobacterium속 균수는, 무급여소의 1.02배∼1.18배로 증가하였고, Escherichia coli는 0.77배∼0.16배로 감소하였고, Clostridium perfringens는 0.89배∼0.70배로 감소했다.As shown in Table 5, the number of bacteria of the genus Bifidobacterium in the manure of cows fed with the useful bacteria increasing agent of Examples 1 to 5 according to the present invention increased from 1.02 to 1.18 times that of unfed cattle, and 0.77 to 0.16 times for Escherichia coli. decreased by a factor of two, and Clostridium perfringens decreased by 0.89 to 0.70 times.

이에 대하여 표6에 나타나 있는 바와 같이, 비교예1∼5에서는 Bifidobacterium속 및 Escherichia coli에 큰 증감은 없고, Clostridium perfringens는 최대 1.22배로 증가했다. 실시예1∼5에서는 Salmonella속 균수가 0.98배∼0.93배로 큰 변화는 없고, Campylobacter jejumi가 1.13배∼1.16배로 약간 증가한 것에 대해, 비교예1∼5에서는 Salmonella속이 1.01∼1.10배, Campylobacter jejumi가 1.17∼1.26배가 되고 있어, 실시예에서는 양자의 균수의 증가를 억제한 것을 알 수 있다.On the other hand, as shown in Table 6, in Comparative Examples 1 to 5, there was no significant increase or decrease in Bifidobacterium genus and Escherichia coli, and Clostridium perfringens increased by a maximum of 1.22 times. In Examples 1-5, the number of bacteria of the genus Salmonella did not change significantly, from 0.98 times to 0.93 times, and Campylobacter jejumi increased slightly to 1.13 times to 1.16 times. It is -1.26 times, and it turns out that the increase of the number of both bacteria was suppressed in the Example.

표7에 나타나 있는 바와 같이, 실시예1∼5의 분중의 Lactobacillus속의 검출 마리수의 비율은, L.Casei-group이 20%∼40%, L.gasseri가 40%∼60%이었다.As shown in Table 7, the ratio of the number of detected animals of the genus Lactobacillus in the minutes of Examples 1 to 5 was 20% to 40% for L. Casei-group and 40% to 60% for L. gasseri.

이에 대하여 표8에 나타나 있는 바와 같이, 비교예1∼5에서는 어느 균도 검출된 개체는 없었다.In contrast, as shown in Table 8, no bacteria were detected in Comparative Examples 1 to 5.

또한 표9에 나타나 있는 바와 같이, 실시예1∼5의 설사 발생률은 0∼20%이었다.In addition, as shown in Table 9, the diarrhea incidence rate in Examples 1 to 5 was 0 to 20%.

이에 대하여 비교예1∼5에서는 40∼80%로, 실시예1∼5보다 설사 발생률이 높았다.On the other hand, in Comparative Examples 1-5, it was 40-80%, and the diarrhea incidence rate was higher than that of Examples 1-5.

표10에 나타나 있는 바와 같이, 실시예1∼5에서는 젖생산량이 37∼39kg/일로 높았다.As shown in Table 10, in Examples 1 to 5, the milk production was as high as 37 to 39 kg/day.

이에 대하여 비교예1∼5에서는 33∼35kg/일로, 실시예1∼5보다 젖생산량이 낮았다.On the other hand, in Comparative Examples 1-5, it was 33-35 kg/day, and the milk production amount was lower than Examples 1-5.

〔실시예6∼10, 비교예6∼10〕[Examples 6-10, Comparative Examples 6-10]

물 및 표11에 나타낸 기초사료를 포식, 급여하고 있는 ＬＷＤ종 거세 새끼돼지 50마리를 실시예 및 비교예별로 5마리 사용하고, 표12 및 표13에 나타낸 가축용 사료를 2주간 급여했다. 급여 2주 후에 분을 샘플링하고, 분석하기까지 동결 보존했다. 분중의 세균수는 리얼타임 ＰＣＲ법에 의하여 Bifidobacterium속, Escherichia coli, Salmonella속, Campylobacter jejumi., Clostridium perfringens에 대해서 조사했다. 구체적으로는, 1)검체로부터 ＤＮＡ를 추출, 2)그 ＤＮＡ를 사용해서 각 ＰＣＲ법으로 분석, 3)유전자량과 세균수와의 비례관계에 의거하여 세균수를 정량하는 순서에 따라 세균수를 구하였다. 그리고 급여하기 전의 돼지의 세균수를 1로 한 상대값으로서 산출했다. 또한 멀티플렉스 ＰＣＲ법에 의하여 분변중의 Lactobacillus속 세균 9종류, 즉 Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus reuteri의 검출을 하였다. 또한 급여기간중의 설사 발생률과, 급여 2주간의 사료섭취량과 체중으로부터 사료효율을 산출했다. 각 실시예의 결과를 표14, 표16, 표18 및 표19에, 각 비교예의 결과를 표15, 표17, 표18 및 표19에 각각 나타낸다.50 castrated piglets of LLD species, which were fed and fed with water and the basic feed shown in Table 11, were used for each Example and Comparative Example, and the feed for livestock shown in Tables 12 and 13 was fed for 2 weeks. Minutes were sampled 2 weeks after feeding and cryopreserved until analysis. Bifidobacterium, Escherichia coli, Salmonella, Campylobacter jejumi., and Clostridium perfringens were investigated for the number of bacteria in the manure by real-time PRC method. Specifically, the number of bacteria is determined in the following order: 1) extracting DN from the specimen, 2) using the DN and analyzing it by each PRC method, 3) quantifying the number of bacteria based on the proportional relationship between the amount of gene and the number of bacteria. saved Then, the number of bacteria in pigs before feeding was calculated as a relative value with 1. In addition, 9 types of Lactobacillus genus bacteria in feces, namely Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus reuteri, were detected by the multiplex PRC method. In addition, the feed efficiency was calculated from the incidence of diarrhea during the feeding period and the feed intake and body weight for 2 weeks of feeding. The results of each Example are shown in Table 14, Table 16, Table 18 and Table 19, and the results of each comparative example are shown in Table 15, Table 17, Table 18 and Table 19, respectively.

기초사료의 배합비율Basic feed mixing ratio 원료Raw material 배합비율（％）Blending ratio (%) 옥수수corn ５５．２55．2 마일로(Milo)Milo ２０．０20.0 탈지쌀겨skim rice bran １６．０16. 0 어분fishmeal ６．０６．0 비타민미네랄믹스vitamin and mineral mix ２．８２．8

실시예Example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 카프릴산Ｃａcaprylic acid －- 0.2%0.2% －- 0.1%0.1% －- 카프르산Ｃａcapric acid 1.0%1.0% 0.2%0.2% 0.2%0.2% 0.1%0.1% －- 라우르산ＣａLauric acid C －- －- 0.2%0.2% 0.1%0.1% －- 카프릴산·카프르산·라우르산 혼합 지방산CaMixed fatty acid Ca of caprylic acid, capric acid, and lauric acid －- －- －- －- 0.5%0.5%

비교예comparative example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 카프르산Ｃａcapric acid 0.05%0.05% －- －- －- －- 라우르산CaCa lauric acid －- 0.08%0.08% －- －- －- 대두유 지방산CaSoybean Oil Fatty Acid Ca －- －- 2%2% －- －- 채종유 지방산CaRape Oil Fatty Acid Ca －- －- －- 3%3% －- 쌀기름 지방산CaRice Oil Fatty Acid Ca －- －- －- －- 5%5%



분중 균수 상대값The relative value of the number of germs in the minute 실시예Example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 Bifidobacterium속genus Bifidobacterium 1.321.32 1.261.26 1.161.16 1.301.30 1.351.35 Escherichia coliEscherichia coli 0.330.33 0.520.52 0.790.79 0.430.43 0.260.26 Salmonella속Salmonella 0.310.31 0.660.66 0.840.84 0.560.56 0.210.21 Campylobacter jejuniCampylobacter jejuni 0.640.64 0.720.72 0.880.88 0.820.82 0.600.60 Clostridium perfringensClostridium perfringens 0.700.70 0.770.77 0.890.89 0.800.80 0.660.66



분중 균수 상대값The relative value of the number of germs in the minute 비교예comparative example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 Bifidobacterium속genus Bifidobacterium 0.980.98 0.990.99 1.001.00 0.980.98 0.930.93 Escherichia coliEscherichia coli 1.031.03 1.051.05 1.131.13 1.001.00 1.111.11 Salmonella속Salmonella 1.081.08 1.051.05 1.141.14 1.081.08 1.161.16 Campylobacter jejuniCampylobacter jejuni 1.151.15 1.161.16 1.271.27 1.201.20 1.161.16 Clostridium perfringensClostridium perfringens 1.101.10 1.081.08 1.291.29 1.251.25 1.251.25



분중 Lactobacillus속 검출 마릿수／５마리Number of Lactobacillus genus detected per minute／5 실시예Example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 L. acidophilusL. acidophilus 33 33 22 33 33 L. casei-groupL. casei-group 00 00 00 00 00 L. delbrueckiiL. delbrueckii 33 33 1One 33 44 L. gasseriL. gasseri 44 33 33 44 44 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 33 33 22 33 33 L. reuteriL. reuteri 00 00 00 00 00



분중 Lactobacillus속 검출 마릿수／５마리Number of Lactobacillus genus detected per minute／5 비교예comparative example No.6No.6 No.7No.7 No.8No.8 No.9No.9 No.10No.10 L. acidophilusL. acidophilus 00 1One 00 00 00 L. casei-groupL. casei-group 00 00 00 00 00 L. delbrueckiiL. delbrueckii 1One 1One 00 00 00 L. gasseriL. gasseri 1One 1One 00 00 00 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 00 1One 00 00 00 L. reuteriL. reuteri 00 00 00 00 00

설사발생률（%）Diarrhea incidence (%)

실시예

Example No.6No.6 0（0/5）0 (0/5) No.7No.7 0（0/5）0 (0/5) No.8No.8 20（1/5）20 (1/5) No.9No.9 0（0/5）0 (0/5) No.10No.10 0（0/5）0 (0/5)

비교예

comparative example No.6No.6 40（2/5）40 (2/5) No.7No.7 40（2/5）40 (2/5) No.8No.8 80（4/5）80 (4/5) No.9No.9 80（4/5）80 (4/5) No.10No.10 80（4/5）80 (4/5)

사료효율Feed efficiency

실시예

Example No.6No.6 0.840.84 No.7No.7 0.820.82 No.8No.8 0.810.81 No.9No.9 0.820.82 No.10No.10 0.840.84

비교예

comparative example No.6No.6 0.790.79 No.7No.7 0.780.78 No.8No.8 0.770.77 No.9No.9 0.790.79 No.10No.10 0.770.77

표14에 나타나 있는 바와 같이, 실시예6∼10에서는, 분중의 Bifidobacterium속이 1.16배∼1.35배로 증가하고, Escherichia coli가 0.79배∼0.26배, Salmonella속이 0.84배∼0.21배, Campylobacter jejumi가 0.88배∼0.60배, Clostridium pefringens가 0.89배∼0.66배로 감소했다.As shown in Table 14, in Examples 6-10, the genus Bifidobacterium in the manure increased by 1.16 to 1.35 times, Escherichia coli 0.79 to 0.26, Salmonella 0.84 to 0.21, and Campylobacter jejumi 0.88 to 0.88. 0.60 times and Clostridium pefringens decreased by 0.89 to 0.66 times.

이에 대하여 표15에 나타나 있는 바와 같이, 비교예6∼10의 경우, Biffidobacterium속이 0.93∼1.00배로 큰 변화는 없고, Escherichia coli가 1.00배∼1.13배, Salmonella속이 1.05배∼1.16배, Campylobacter jejumi가 1.15∼1.27배, Clostridium perfringens가 1.08∼1.29배로 증가했다.On the other hand, as shown in Table 15, in Comparative Examples 6 to 10, there was no significant change in Biffidobacterium genus 0.93 to 1.00 times, Escherichia coli 1.00 times to 1.13 times, Salmonella genus 1.05 times to 1.16 times, Campylobacter jejumi 1.15 times. ∼1.27 times, Clostridium perfringens increased by 1.08∼1.29 times.

표16에 나타나 있는 바와 같이, 실시예6∼10의 분중의 Lactobacillus속의 검출 마릿수의 비율(검출률)은, L.acidophilus가 40%∼60%, L.delbrueckii가 20%∼80%, L.gasseri가 60%∼80%, L.rhamnosus가 40%∼60%이었다.As shown in Table 16, the ratio (detection rate) of the number of detected animals of the genus Lactobacillus per minute in Examples 6 to 10 was 40% to 60% for L.acidophilus, 20% to 80% for L.delbrueckii, and 20% to 80% for L. gasseri. was 60% to 80%, and L. rhamnosus was 40% to 60%.

이에 대하여 표17에 나타나 있는 바와 같이, 비교예6∼10에서는 L.acidophilus가 0%∼20%, L.delbrueckii에서 0%∼20%, L.gasseri에서 0%∼20%, L.rhamnosus에서 0%∼20%로 검출률은 낮았고, 또한 다른 균에 대해서는 검출되지 않았다.In contrast, as shown in Table 17, in Comparative Examples 6 to 10, L. acidophilus was 0% to 20%, L. delbrueckii was 0% to 20%, L. gasseri was 0% to 20%, and L. rhamnosus was The detection rate was low at 0% to 20%, and no other bacteria were detected.

또한 표18에 나타나 있는 바와 같이, 실시예6∼10의 설사 발생률은 0∼20%이었다.Further, as shown in Table 18, the diarrhea incidence rate in Examples 6 to 10 was 0 to 20%.

이에 대하여 비교예6∼10에서는 40∼80%로, 실시예6∼10보다 설사 발생률이 높았다.On the other hand, in Comparative Examples 6 to 10, 40 to 80%, the incidence of diarrhea was higher than in Examples 6 to 10.

또한 표19에 나타나 있는 바와 같이, 실시예6∼10의 사료효율은 0.81∼0.84로 높았다.Also, as shown in Table 19, the feed efficiency of Examples 6 to 10 was as high as 0.81 to 0.84.

이에 대하여 비교예6∼10에서는 0.77에서 0.79로, 실시예6∼10보다 사료효율이 낮았다.On the other hand, in Comparative Examples 6-10, the feed efficiency was lower than that of Examples 6-10, from 0.77 to 0.79.

〔실시예11∼15, 비교예11∼15〕[Examples 11-15, Comparative Examples 11-15]

물 및 표20에 나타낸 기초사료를 포식, 급여하고 있는 3주 나이의 브로일러(broiler) 1000마리를 실시예 및 비교예별로 100마리 사용하고, 표21 및 표22에 나타낸 가축용 사료를 5주간 급여했다. 급여 5주 후에 각 섹션 5마리씩으로부터 분을 샘플링하고, 분석하기까지 동결 보존했다. 분중의 세균수는, 리얼타임 ＰＣＲ법에 의하여 Bifidobacterium속, Escherichia coli, Salmonella속, Campylobacter jejumi., Clostridium perfringens에 대해서 조사했다. 구체적으로는, 1)검체로부터 ＤＮＡ를 추출, 2)그 ＤＮＡ를 사용해서 각 ＰＣＲ법으로 분석, 3)유전자량과 세균수와의 비례관계에 의거하여 세균수를 정량하는 순서에 따라 세균수를 구하였다.1000 broilers of 3 weeks age who were fed and fed with water and the basic feed shown in Table 20 were used for each Example and Comparative Example, and the feed for livestock shown in Tables 21 and 22 was fed for 5 weeks. did. Minutes were sampled from 5 each section 5 weeks after feeding and cryopreserved until analysis. Bifidobacterium genus, Escherichia coli, Salmonella genus, Campylobacter jejumi., and Clostridium perfringens were investigated for the number of bacteria in the manure by real-time PRC method. Specifically, the number of bacteria is determined in the following order: 1) extracting DNA from the sample, 2) using the DNA and analyzing it by each PRC method, 3) quantifying the number of bacteria based on the proportional relationship between the amount of gene and the number of bacteria saved

그리고 급여하기 전의 브로일러의 세균수를 1로 한 상대값으로서 산출했다. 또한 멀티플렉스 ＰＣＲ법에 의하여, 분변중의 Lactobacillus속 세균 9종류, 즉 Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus reuteri의 검출을 하였다. 또한 급여 5주 후의 육성률을 조사했다. 각 실시예의 결과를 표23, 표25 및 표27에, 각 비교예의 결과를 표24, 표26 및 표27에 각각 나타낸다.And it computed as the relative value which made the number of bacteria of the broiler before feeding to 1. In addition, 9 types of Lactobacillus genus bacteria in feces, namely Lactobacillus acidophilus, Lactobacillus casei-group, Lactobacillus delbrueckii, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus reuteri, were detected by the multiplex PRC method. In addition, the growth rate after 5 weeks of salary was investigated. The results of each Example are shown in Tables 23, 25 and 27, and the results of each comparative example are shown in Tables 24, 26 and 27, respectively.

기초사료의 배합비율Basic feed mixing ratio 원료Raw material 배합비율（％）Mixing ratio (%) 마일로Milo ４８．３48．３ 보리barley ３０．０30.0 대두박(soybean cake)soybean cake １０．５10. 5 어분fishmeal ８．０8．0 비타민미네랄믹스vitamin and mineral mix ３．２３．２

실시예Example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 카프릴산Ｃａcaprylic acid 1.0%1.0% 0.2%0.2% 0.2%0.2% －- －- 카프르산Ｃａcapric acid －- －- 0.2%0.2% －- －- 라우르산ＣａLauric acid C －- 0.2%0.2% 0.2%0.2% －- －- 카프릴산·카프르산·라우르산 혼합 지방산CaMixed fatty acid Ca of caprylic acid, capric acid, and lauric acid －- －- －- 1.0%1.0% 0.1%0.1%

비교예comparative example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 카프릴산Ｃａcaprylic acid 0.09%0.09% －- －- －- －- 카프릴산·카프르산·라우르산 혼합 지방산CaMixed fatty acid Ca of caprylic acid, capric acid, and lauric acid －- 0.05%0.05% －- －- －- 스테아린산CaCa stearate －- －- 1%One% －- －- 팔미트산CaPalmitate Ca －- －- －- 5%5% －- 올레산CaOleic acid Ca －- －- －- －- 3%3%



분중 균수 상대값The relative value of the number of germs in the minute 실시예Example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 Bifidobacterium속genus Bifidobacterium 1.241.24 1.201.20 1.221.22 1.311.31 1.161.16 Escherichia coliEscherichia coli 0.420.42 0.580.58 0.500.50 0.400.40 0.770.77 Salmonella속Salmonella 0.350.35 0.440.44 0.380.38 0.230.23 0.680.68 Campylobacter jejuniCampylobacter jejuni 0.640.64 0.750.75 0.670.67 0.630.63 0.800.80 Clostridium perfringensClostridium perfringens 0.700.70 0.800.80 0.730.73 0.640.64 0.850.85



분중 균수 상대값The relative value of the number of germs in the minute 비교예comparative example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 Bifidobacterium속genus Bifidobacterium 1.011.01 1.001.00 0.990.99 0.980.98 0.980.98 Escherichia coliEscherichia coli 1.021.02 1.071.07 1.111.11 1.051.05 0.990.99 Salmonella속Salmonella 1.041.04 1.041.04 1.111.11 1.091.09 1.091.09 Campylobacter jejuniCampylobacter jejuni 1.161.16 1.111.11 1.091.09 1.081.08 1.001.00 Clostridium perfringensClostridium perfringens 1.051.05 1.001.00 1.031.03 0.990.99 1.101.10



분중 Lactobacillus속 검출 마릿수／５마리Number of Lactobacillus genus detected per minute／5 실시예Example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 L. acidophilusL. acidophilus 22 33 22 22 33 L. casei-groupL. casei-group 00 00 00 00 00 L. delbrueckiiL. delbrueckii 33 33 22 22 33 L. gasseriL. gasseri 33 44 33 22 44 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 44 22 33 33 33 L. reuteriL. reuteri 00 00 00 00 00



분중 Lactobacillus속 검출 마릿수／５마리Number of Lactobacillus genus detected per minute／5 비교예comparative example No.11No.11 No.12No.12 No.13No.13 No.14No.14 No.15No.15 L. acidophilusL. acidophilus 00 00 00 00 00 L. casei-groupL. casei-group 00 00 00 00 00 L. delbrueckiiL. delbrueckii 00 00 00 1One 00 L. gasseriL. gasseri 00 00 1One 00 00 L. plantarumL. plantarum 00 00 00 00 00 L. rhamnosusL. rhamnosus 00 00 00 00 00 L. reuteriL. reuteri 00 00 00 00 00

육성률（%）Growth rate (%)

실시예

Example No.11No.11 100100 No.12No.12 9999 No.13No.13 100100 No.14No.14 100100 No.15No.15 9999

비교예

comparative example No.11No.11 9797 No.12No.12 9696 No.13No.13 9696 No.14No.14 9595 No.15No.15 9595

표23에 나타나 있는 바와 같이, 실시예11∼15에서는, 분중의 Bifidobacterium속이 1.16배∼1.31배로 증가하였고, Escherichia coli가 0.77배∼0.40배, Salmonella속이 0.68배∼0.23배, Campylobacter jejumi가 0.80배∼0.63배, Clostridium pefringens가 0.85배∼0.64배로 감소했다.As shown in Table 23, in Examples 11-15, the genus Bifidobacterium in the manure increased by 1.16 to 1.31 times, Escherichia coli by 0.77 to 0.40, Salmonella by 0.68 to 0.23, and Campylobacter jejumi by 0.80 to 0.80. 0.63 times and Clostridium pefringens decreased by 0.85 to 0.64 times.

이에 대하여 표24에 나타나 있는 바와 같이, 비교예11∼15의 경우, Bifidobacterium속이 0.98∼1.01배로 큰 변화는 없고, Escherichia coli가 0.99배∼1.11배, Salmonella속이 1.04배∼1.11배, Campylobacter jejumi가 1.00∼1.16배, Clostridium perfringens가 0.99∼1.10배로 큰 변화가 없거나, 또는 증가했다.On the other hand, as shown in Table 24, in Comparative Examples 11 to 15, there was no significant change in Bifidobacterium genus 0.98 to 1.01 fold, Escherichia coli 0.99 to 1.11 fold, Salmonella genus 1.04 to 1.11 fold, Campylobacter jejumi 1.00 ∼1.16 times, Clostridium perfringens showed no significant change or increased by 0.99∼1.10 times.

표25에 나타나 있는 바와 같이, 실시예11∼15의 분중의 Lactobacillus속의 검출 마릿수의 비율(검출율)은, L.acidophilus가 40%∼60%, L.delbrueckii가 40%∼60%, L.gasseri가 40%∼80%, L.rhamnosus가 40%∼80% 검출되었다.As shown in Table 25, the ratio (detection rate) of the number of detected animals of the genus Lactobacillus per minute in Examples 11 to 15 was 40% to 60% for L. acidophilus, 40% to 60% for L. delbrueckii, and 40% to 60% for L. gasseri was detected in 40% to 80% and L. rhamnosus in 40% to 80%.

이에 대하여 표26에 나타나 있는 바와 같이, 비교예11∼15에서는 L.delbrueckii가 0%∼20%, L.gasseri에서 0%∼20%로 검출율은 낮았고, 또한 다른 균에 대해서는 검출되지 않았다.In contrast, as shown in Table 26, in Comparative Examples 11 to 15, L. delbrueckii was 0% to 20% and L. gasseri was 0% to 20%, and the detection rate was low, and no other bacteria were detected.

또한 표27에 나타나 있는 바와 같이, 실시예11∼15의 육성률은 99∼100%이었다.Further, as shown in Table 27, the growth rates of Examples 11 to 15 were 99 to 100%.

이에 대하여 비교예11∼15에서는 95∼97%로, 실시예11∼15보다 육성률이 낮았다.
On the other hand, in Comparative Examples 11-15, it was 95-97%, and the growth rate was lower than Examples 11-15.

본 발명의 동물장내 유용균 증가제는, 동물의 장내에 존재하는 유용균을 증가시키고 또한 유해균을 감소시킴으로써 동물의 장내 환경을 개선시킬 수 있기 때문에, 식품첨가제, 의약품으로서 사용할 수 있고, 또 가축용 사료의 첨가제로서도 사용할 수 있다. 특히, 본 발명의 동물장내 유용균 증가제가 특정량 배합된 사료를 가축에 급여함으로써, 가축이 구비하는 장내 세균총내에 유용균을 증가시키고 또한 유해균을 감소시킴으로써 가축의 장내 환경을 개선시켜, 이에 따라 질병의 발생을 미연에 방지하여고, 가축의 생산성을 향상시킬 수 있다.Since the useful bacteria increasing agent in the animal intestine of the present invention can improve the intestinal environment of animals by increasing useful bacteria present in the intestine of animals and reducing harmful bacteria, it can be used as a food additive or pharmaceutical, and can be used as a feed for livestock. It can also be used as an additive. In particular, by feeding livestock a feed formulated with a specific amount of the beneficial bacteria increasing agent in the animal gut of the present invention, the intestinal environment of livestock is improved by increasing the useful bacteria in the intestinal flora provided by the livestock and reducing harmful bacteria, thereby improving the occurrence of diseases can be prevented in advance, and the productivity of livestock can be improved.

Claims (4)

카프릴산(caprylic acid), 카프르산(capric acid) 및 라우르산(lauric acid)의 지방산 또는 그 염을 함유하고, 카프릴산 : 카프르산 : 라우르산의 중량비가 20 : 20 : 60인, 동물장내 유용균수의 절대값을 증가시키는 동물장내 유용균 증가제(動物腸內 有用菌 增加劑).
It contains fatty acids or salts thereof of caprylic acid, capric acid and lauric acid, and the weight ratio of caprylic acid: capric acid: lauric acid is 20: 20: A 60-person, effective bacteria-increasing agent that increases the absolute number of useful bacteria in the animal gut.
 제1항에 있어서,
지방산의 염이 칼슘염인 동물장내 유용균 증가제.
According to claim 1,
An agent for increasing useful bacteria in the intestines of animals whose fatty acid salt is a calcium salt.
 제1항 또는 제2항에 있어서,
장내 유용균이 Bifidobacterium속(屬)에 속하는 균(菌)인 동물장내 유용균 증가제.
3. The method of claim 1 or 2,
An agent for increasing useful bacteria in the intestines of animals, the beneficial bacteria belonging to the genus Bifidobacterium.
 제1항 또는 제2항의 동물장내 유용균 증가제가 0.1∼2중량％ 배합된 사료를 가축에 급여하는 것을 특징으로 하는 가축장내 환경개선방법.A method for improving the environment in a livestock field, characterized in that the feed containing 0.1 to 2% by weight of the beneficial bacteria increasing agent in the animal intestine according to claim 1 or 2 is fed to the livestock.
KR1020177018724A 2015-02-13 2015-02-13 Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used KR102376381B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2015/053961 WO2016129100A1 (en) 2015-02-13 2015-02-13 Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used

Publications (2)

Publication Number Publication Date
KR20170117382A KR20170117382A (en) 2017-10-23
KR102376381B1 true KR102376381B1 (en) 2022-03-17

Family

ID=56614466

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177018724A KR102376381B1 (en) 2015-02-13 2015-02-13 Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used

Country Status (3)

Country Link
JP (1) JP6557686B2 (en)
KR (1) KR102376381B1 (en)
WO (1) WO2016129100A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3508068A4 (en) * 2016-09-02 2019-10-09 FUJIFILM Corporation Livestock feed or livestock supplement, lactobacillus bacteria growth promoter, and lactobacillus bacteria growth promotion method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020094346A1 (en) 1995-05-17 2002-07-18 M. D. Henry C. Lin Method and compositions for improving digestion and absorption in the small intestine
JP2003535894A (en) 2000-06-20 2003-12-02 エヌ・ブイ・ニュートリション・サイエンシス Medium-chain fatty acids applicable as antibacterial agents
JP3480566B2 (en) 1998-08-25 2003-12-22 日本水産株式会社 Natural bioactive substance effective for fish disease and fish feed containing it
JP2005270101A (en) 2004-02-27 2005-10-06 Nippon Suisan Kaisha Ltd Feed for eel
WO2011135564A1 (en) 2010-04-26 2011-11-03 Enzymotec Ltd. Methods and lipid compositions for promoting development of gut flora
US20130028869A1 (en) 2010-04-14 2013-01-31 Nutrition Sciences Nv/Sa Feed supplement comprising oligosaccharides and medium chain fatty acids
US20140037698A1 (en) 2011-01-21 2014-02-06 Norel, S.A Additives for animal food

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2723961B2 (en) 1989-03-31 1998-03-09 花王株式会社 Veterinary milk substitute composition and breeding method
JP3203441B2 (en) 1992-11-19 2001-08-27 ミヨシ油脂株式会社 Livestock nutritional supplement
JPH10127232A (en) 1996-10-29 1998-05-19 Natl Fedelation Of Agricult Coop Assoc Substitute milk composition for juvenile livestock and raising of juvenile livestock
JP2002518031A (en) * 1998-06-23 2002-06-25 ユニバーシティ オブ メリーランド Use of oils with high lauric acid content as animal feed
EP1059041A1 (en) * 1999-06-07 2000-12-13 Universiteit Gent The combined use of triglycerides containing medium chain fatty acids and exogenous lipolytic enzymes as feed supplements
EP1314358A1 (en) * 2001-11-21 2003-05-28 N.V. Seghers Nutrition Sciences Antimicrobial composition for animals
KR20100108526A (en) * 2007-12-04 2010-10-07 웨스트게이트 바이올로지컬 리미티드 Antimicrobial compositions comprising fatty acids and milk proteins
JP2011062093A (en) * 2009-09-15 2011-03-31 Kaneka Corp Composition for food and/or feed

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020094346A1 (en) 1995-05-17 2002-07-18 M. D. Henry C. Lin Method and compositions for improving digestion and absorption in the small intestine
JP3480566B2 (en) 1998-08-25 2003-12-22 日本水産株式会社 Natural bioactive substance effective for fish disease and fish feed containing it
JP2003535894A (en) 2000-06-20 2003-12-02 エヌ・ブイ・ニュートリション・サイエンシス Medium-chain fatty acids applicable as antibacterial agents
JP5259905B2 (en) * 2000-06-20 2013-08-07 エヌ・ブイ・ニュートリション・サイエンシス Medium chain fatty acids applicable as antibacterial agents
JP2005270101A (en) 2004-02-27 2005-10-06 Nippon Suisan Kaisha Ltd Feed for eel
US20130028869A1 (en) 2010-04-14 2013-01-31 Nutrition Sciences Nv/Sa Feed supplement comprising oligosaccharides and medium chain fatty acids
WO2011135564A1 (en) 2010-04-26 2011-11-03 Enzymotec Ltd. Methods and lipid compositions for promoting development of gut flora
KR101411679B1 (en) 2010-04-26 2014-06-25 엔지모테크 리미티드 Methods and lipid compositions for promoting development of gut flora
US20140037698A1 (en) 2011-01-21 2014-02-06 Norel, S.A Additives for animal food

Also Published As

Publication number Publication date
WO2016129100A1 (en) 2016-08-18
KR20170117382A (en) 2017-10-23
JP6557686B2 (en) 2019-08-07
JPWO2016129100A1 (en) 2017-11-24

Similar Documents

Publication Publication Date Title
US10398156B2 (en) Animal feed compositions and feed additives
US10834942B2 (en) Yeast and bacterial probiotics combinations and methods of use to improve swine production
US20030198730A1 (en) Food supplement and use thereof for elevating levels of essential fatty acids in livestock and products therefrom
CA2748979C (en) Sodium stearoyl-2-lactylate derivatives as an animal feed additive for improving fat utilization efficiency
CA2444189C (en) A food supplement containing essential fatty acids and products therefrom
US20110189347A1 (en) Use of succinic acid
KR20170051592A (en) Fermentation feed for domestic animal comprising liquid fermented sulfuric
EP1314358A1 (en) Antimicrobial composition for animals
KR100857771B1 (en) Compositions for addition to feed for fish comprising Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces serevisiae
KR102376381B1 (en) Agent for increasing useful bacteria in animal intestines, and method for improving intestinal environment in livestock in which said agent is used
TWI663919B (en) Increasing agent for useful bacteria in animal intestines and livestock intestinal environment improvement method using the same
Dharmayudha et al. Soil worms (Lumbricus rubellus) as feed additives for piglets' growth, blood profile and immunomodulators
KR100877800B1 (en) Feed Composition for Livestock
Dayani et al. Effect of feeding dietary treated wheat straw with urea and whey on fattening lambs performance.
KAHRAMAN et al. Determination of in vitro digestibility and some quality characteristics of fermented sucuk foods produced for dogs
Dabiri et al. Effect of Different Levels of Biosaf Probiotic in Medium Concentrate Diet on Performance and Blood Factors of Iranian Zandi Lambs. J Fisheries Livest Prod 4: 206 doi: 10.4172/2332-2608.1000206 Page 2 of 4 was recorded daily and the residues were collected and weighed. Feed conversion ratio was worked out by dividing the feed intake (g/day) by weight gain (g/day). Blood samples were collected from jugular veins by vacutainer tubes containing heparin as anticoagulant at beginning and on d 42 and d 84, approximately 3 h after the morning feeding and transported to the laboratory till analyzed for total protein, globulin, albumin, BUN, white blood cells and heterophil: lymphocyte ratio according to standard methods. All statistical analyses were conducted by GLM procedure for the completely randomized design with three treatments and three replicates using the SAS software (SAS, 1997) and the means were compared using Duncan's Multiple Range test at the level of P< 0.05
Flutura et al. The effect of pellet and granulated feed on production parameters of fattening pigs.
Caisîn et al. The Influence of the preparation primix Bionorm K on the digestibility of the nutrients in the fodders for young pigs
JP2009106213A (en) Cholesterol-reducing agent for pet, and cholesterol-reducing pet foods

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
AMND Amendment
X701 Decision to grant (after re-examination)
GRNT Written decision to grant