CN112526123A - 一种dnp-bsa聚合度的质控方法 - Google Patents
一种dnp-bsa聚合度的质控方法 Download PDFInfo
- Publication number
- CN112526123A CN112526123A CN202011297476.8A CN202011297476A CN112526123A CN 112526123 A CN112526123 A CN 112526123A CN 202011297476 A CN202011297476 A CN 202011297476A CN 112526123 A CN112526123 A CN 112526123A
- Authority
- CN
- China
- Prior art keywords
- dnp
- bsa
- pad
- polymerization
- degree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nanotechnology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
原料 | 货号 | 产家 |
胶体金 | G7277 | Sigma |
碳酸钾溶液 | / | 市售 |
DNP-BSA | / | Sigma |
BSA | 69003431 | 国药试剂 |
PEG20000 | / | Sigma |
Tris缓冲液 | T6455 | Sigma |
吐温20 | 93773 | Sigma |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011297476.8A CN112526123B (zh) | 2020-11-18 | 2020-11-18 | 一种dnp-bsa聚合度的质控方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011297476.8A CN112526123B (zh) | 2020-11-18 | 2020-11-18 | 一种dnp-bsa聚合度的质控方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112526123A true CN112526123A (zh) | 2021-03-19 |
CN112526123B CN112526123B (zh) | 2022-05-24 |
Family
ID=74981527
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011297476.8A Active CN112526123B (zh) | 2020-11-18 | 2020-11-18 | 一种dnp-bsa聚合度的质控方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112526123B (zh) |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2195328A1 (fr) * | 1994-09-28 | 1996-04-04 | Jean Kadouche | Diagnostic direct d'haptenes |
CN1645146A (zh) * | 2005-02-03 | 2005-07-27 | 厦门大学 | 用荧光稀土纳米颗粒作为标记物的免疫层析方法及其检测试纸条 |
CN200986547Y (zh) * | 2005-04-15 | 2007-12-05 | 华南农业大学 | 庆大霉素快速半定量检测试纸 |
WO2010101777A1 (en) * | 2009-03-06 | 2010-09-10 | Idexx Laboratories, Inc. | Detection of melamine |
CN101900728A (zh) * | 2010-08-05 | 2010-12-01 | 中国农业科学院油料作物研究所 | 半定量化检测黄曲霉毒素b1的多检测线免疫层析试纸条及其制备方法 |
CN102901814A (zh) * | 2012-07-12 | 2013-01-30 | 浙江大学 | 莠去津分子印迹金标试纸条及其用途 |
CN103869079A (zh) * | 2014-03-06 | 2014-06-18 | 瑞莱生物工程(深圳)有限公司 | 一种快速定量检测半乳糖凝集素-3的胶体金试纸 |
CN103901216A (zh) * | 2014-04-22 | 2014-07-02 | 上海科华生物工程股份有限公司 | 人h-fabp胶体金检测试纸及其制备方法 |
CN106066399A (zh) * | 2016-05-24 | 2016-11-02 | 深圳市前海安测信息技术有限公司 | 检测早期糖尿病肾病的胶体金试纸条的制备方法 |
CN107907679A (zh) * | 2017-11-06 | 2018-04-13 | 南京诺唯赞医疗科技有限公司 | 一种免疫层析试纸条及其制作方法与应用 |
CN108279309A (zh) * | 2018-01-30 | 2018-07-13 | 深圳市伯劳特生物制品有限公司 | 一种pla2r抗体的检测试纸条及检测方法 |
CN109187981A (zh) * | 2018-08-01 | 2019-01-11 | 万东山 | 一种快速检测β-淀粉样蛋白的量子点免疫层析试纸条与应用 |
-
2020
- 2020-11-18 CN CN202011297476.8A patent/CN112526123B/zh active Active
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2195328A1 (fr) * | 1994-09-28 | 1996-04-04 | Jean Kadouche | Diagnostic direct d'haptenes |
CN1645146A (zh) * | 2005-02-03 | 2005-07-27 | 厦门大学 | 用荧光稀土纳米颗粒作为标记物的免疫层析方法及其检测试纸条 |
CN200986547Y (zh) * | 2005-04-15 | 2007-12-05 | 华南农业大学 | 庆大霉素快速半定量检测试纸 |
WO2010101777A1 (en) * | 2009-03-06 | 2010-09-10 | Idexx Laboratories, Inc. | Detection of melamine |
CN101900728A (zh) * | 2010-08-05 | 2010-12-01 | 中国农业科学院油料作物研究所 | 半定量化检测黄曲霉毒素b1的多检测线免疫层析试纸条及其制备方法 |
CN102901814A (zh) * | 2012-07-12 | 2013-01-30 | 浙江大学 | 莠去津分子印迹金标试纸条及其用途 |
CN103869079A (zh) * | 2014-03-06 | 2014-06-18 | 瑞莱生物工程(深圳)有限公司 | 一种快速定量检测半乳糖凝集素-3的胶体金试纸 |
CN103901216A (zh) * | 2014-04-22 | 2014-07-02 | 上海科华生物工程股份有限公司 | 人h-fabp胶体金检测试纸及其制备方法 |
CN106066399A (zh) * | 2016-05-24 | 2016-11-02 | 深圳市前海安测信息技术有限公司 | 检测早期糖尿病肾病的胶体金试纸条的制备方法 |
CN107907679A (zh) * | 2017-11-06 | 2018-04-13 | 南京诺唯赞医疗科技有限公司 | 一种免疫层析试纸条及其制作方法与应用 |
CN108279309A (zh) * | 2018-01-30 | 2018-07-13 | 深圳市伯劳特生物制品有限公司 | 一种pla2r抗体的检测试纸条及检测方法 |
CN109187981A (zh) * | 2018-08-01 | 2019-01-11 | 万东山 | 一种快速检测β-淀粉样蛋白的量子点免疫层析试纸条与应用 |
Non-Patent Citations (1)
Title |
---|
唐雨德等: "金免疫层析法检测囊虫循环抗原试验研究", 《动物医学进展》 * |
Also Published As
Publication number | Publication date |
---|---|
CN112526123B (zh) | 2022-05-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5569608A (en) | Quantitative detection of analytes on immunochromatographic strips | |
US5387503A (en) | Assay method using internal calibration to measure the amount of analyte in a sample | |
EP0279097A2 (en) | Immunoassay test strip | |
IE62974B1 (en) | Method for determination of a polyvalent substance using an immunoaggregate | |
CN108226466B (zh) | 一种免疫层析试纸条及免疫层析检测方法 | |
US5166079A (en) | Analytical assay method | |
CN112526137A (zh) | 一种免疫层析的检测试纸条的制备方法 | |
US4990442A (en) | Assay for an analyte on a solid porous support | |
CN112526123B (zh) | 一种dnp-bsa聚合度的质控方法 | |
CN104610079B (zh) | 一种沙丁胺醇半抗原衍生物、沙丁胺醇人工抗原及其制备方法和应用 | |
CN112986567A (zh) | 一种多项目快速定量毒品检测盒试剂成分配比和制备方法 | |
CN112462052A (zh) | 一种免疫层析的检测试纸条及其使用方法 | |
CN117031022A (zh) | 一种***弹性蛋白酶荧光免疫层析检测试剂盒及检测方法 | |
CN112763728B (zh) | 用于检测液态样品的浓度的检测方法和测试条 | |
CN103543274A (zh) | 一种可视化的生物芯片 | |
Law et al. | Enzyme-linked immunosorbent assay (ELISA) development and optimisation | |
CN111896743A (zh) | 一种荧光免疫层析试纸条及其制备方法和应用 | |
CN100483134C (zh) | 免疫层析装置 | |
CN112881672A (zh) | 通用型多核酸产物检测胶体金试纸条及其制备方法和应用 | |
Rubtsova et al. | Chemiluminescent immunoassay: Application of a portable scanning luminometer for the determination of 2, 4‐dichlorophenoxyacetic acid in microtiter and membrane strip format | |
CN109490537A (zh) | 一种瘦肉精试纸条及其制备方法 | |
CN102520191B (zh) | 一种运用elisa测定蛋白质浓度的方法 | |
CN112881676B (zh) | 食品中塑化剂时间分辨荧光免疫定量层析试纸条制作方法 | |
CN111579774A (zh) | 一种校准、质量控制用的卡式标准物质及其制备方法和应用 | |
US5202432A (en) | Assay for an analyte on a solid porous support |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Tao Inventor after: Chao Pan Inventor after: Zhou Guodong Inventor after: Sheng Buen Inventor before: Zhang Tao Inventor before: Chao Pan Inventor before: Zhou Guodong |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: Building 4, Xiamen Biomedical Industry Collaborative Innovation and Entrepreneurship Center, No. 71 Houxiang Road, Haicang District, Xiamen City, Fujian Province, 361000 Patentee after: XIAMEN TONGRENXIN BIOLOGY TECHNOLOGY Co.,Ltd. Address before: Unit 01, 5th floor, building B12, Xiamen biomedical industrial park, 2072 wengjiao West Road, Haicang District, Xiamen City, Fujian Province, 361000 Patentee before: XIAMEN TONGRENXIN BIOLOGY TECHNOLOGY Co.,Ltd. |