CN1124776A - Process for extracting alcohol from waste liquid in producing saponin with Dioscorea zingiberensis or Dioscorea nipponica - Google Patents
Process for extracting alcohol from waste liquid in producing saponin with Dioscorea zingiberensis or Dioscorea nipponica Download PDFInfo
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- CN1124776A CN1124776A CN 94110810 CN94110810A CN1124776A CN 1124776 A CN1124776 A CN 1124776A CN 94110810 CN94110810 CN 94110810 CN 94110810 A CN94110810 A CN 94110810A CN 1124776 A CN1124776 A CN 1124776A
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Abstract
The process for extracting alcohol from waste liquid of yellow ginger or Rhizoma dioscoreae nipponicae after producing saponin includes such steps as addition of alkaline substance for neutralization to make pH value 4-7, inoculating cultured yeast strain at 28-33 deg.C, addition of N-containing compound, magnesium salt and phosphate before or after inoculation, fermentation at 28-40 deg.C for 60-80 hr, distillation and separation.
Description
The present invention relates to a kind of processing method of extracting alcohol from contain sugared waste liquid, is the method for extracting alcohol from the waste liquid of yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome production saponin specifically.
Existing saponin production technique after to yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome hydrolysis, is slagged tap and waste liquid by the sheet frame separation, and wherein slag is used to extract saponin, and waste liquid is directly emitted.The raw material yellow ginger or the Dioscorea nipponica Mak. Ningpo Yam Rhizome that produce saponin are the higher wild plants of a kind of starch content, after complete hydrolysis, starch wherein just changes glucose into, therefore sugar degree is higher in the waste liquid, general content reaches 3-8%, acidity (in HCL) reaches 4-6%, with in the waste liquid and after, COD, BOD content are considerably beyond emission standard.One ton of saponin of every production will produce 300-400 cubic metres of this organic content height, acid strong waste liquids, if waste liquid is directly emitted, both environment be caused very big pollution, cause the significant wastage of natural resources again.
The objective of the invention is effectively to utilize the sugar in the waste liquid,, resolve into alcohol and carbonic acid gas,,, reduce the pollution level of waste liquid environment to reduce the waste of natural resources again by the distillation extraction alcohol product by saccharomycetic effect.
For achieving the above object, the present invention is with reference to the alcohol producing process of routine, the waste liquid that adopts yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome to produce saponin is made raw material, regulate pH value by neutralization, the control leavening temperature satisfies saccharomycetic growth and breeding condition, and when fermenting to the regular hour, the sugar in the waste liquid just can decompose fully, carry out fractionation by distillation then, obtain alcohol product.If in karusen, replenish an amount of nitrogenous source and trace element, with more favourable saccharomycetic growth and breeding, shorten fermentation period, improve fermented quality.
Fig. 1 is a process flow sheet of the present invention.1. being to adopt the normal temperature yeast to ferment among Fig. 1,2. is to adopt high temperature resistant active dry yeast to ferment, and the two can select one.
Concrete extracting method is as follows:
1, the neutralizing treatment of waste liquid: with yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome hydrolyzed waste or with its concentrated solution, feed neutralization tank, add alkaline matter and be neutralized to pH value 4-7, preferably pH value is 5.0～6.0.If adopting the drystone ashes is neutralizing agent, its add-on is 2～5% (W/V) of waste liquid volume; Determining the add-on of neutralizing agent according to H+ ionic concn in the waste liquid, is good between 5-6 with the control pH value.
2, replenish nitrogenous source and trace element: with the waste liquid suction fermenting container after the neutralizing treatment, according to the sugared total amount that contains in the waste liquid, add an amount of nitrogenous compound, magnesium salts, phosphoric acid salt, replenish nitrogenous source and micro-deficiency in the waste liquid, contain sugared total amount in the waste liquid: nitrogen content in the nitrogenous compound: magnesium content in the magnesium salts: phosphorus content in the phosphoric acid salt=100: 0.025～0.03: 0.005～0.008: 0.015～0.03.As being nitrogenous source to add ammonium sulfate (perhaps urea), be the source of trace element with sal epsom, dipotassium hydrogen phosphate, then their add-on is respectively 0.15% (perhaps 0.05% the urea), 0.05%, 0.1% that contains sugared total amount in the waste liquid.
3, the fermentation of waste liquid: under 28-33 ℃ temperature condition, cultured barms is inserted in above-mentioned pretreated waste liquid, the control leavening temperature is 28～40 ℃, ferments 60～80 hours, can be with the sugar fermentation finished thoroughly1 in the waste liquid.Adopt the normal temperature yeast, inoculation temp is 28～30 ℃, and inoculum size is 10% (V/V) of waste liquid volume, and leavening temperature is 28～32 ℃; If adopt high temperature resistant active dry yeast, inoculation temp is 30～33 ℃, high temperature resistant active dry yeast consumption, and for waste liquid contains 0.08～0.3% (W/W) of sugared total amount, leavening temperature is 30～40 ℃.
4, the extraction of alcohol: with the waste liquid of fermenting-ripening, adopt the conventional production method of alcohol, carry out fractionation by distillation, get final product alcohol product.Poor liquid behind the extraction alcohol, residual sugar content is at (W/V) below 0.3%.
The above-mentioned two yeast-like fungi kinds that adopt, its cultural method is as follows: A, normal temperature yeast are the saccharomyces carlsbergensis bacterial classifications that adopts, the barms cultural method is cultivated in the Alcohol Production routinely, in culturing process, can adopt yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome to produce the saponin hydrolyzed waste and make substratum and replace bent juice of rice or wort, the displaced substratum of institute is used for the enlarged culturing after the triangular flask cultivation.Concrete operations: get waste liquid and be concentrated to sugared content 12～15%, transfer pH value 5.0～5.5 with the drystone ashes, add this waste liquid and contain the ammonium sulfate of sugared total amount 0.15% or 0.05% urea and 0.05% sal epsom, 0.1% hydrogen sulfate dipotassium, under 28-30 ℃ of temperature, insert saccharomyces carlsbergensis bacterial classification through the test tube liquid culture, cultivated 15-24 hours, and got final product further enlarged culturing or do zymophyte.Spawn culture is carried out in the inoculation at once after having transferred pH value of this substratum, does not need to take the sterilization measure, otherwise must carry out sterilising treatment.B, high temperature resistant active dry yeast are a kind of yeast solids, and it is water activation in 2% syrup, can do zymophyte and use; It is 3-5% waste liquid that the present invention takes sugared content, add the drystone ashes and regulate pH value to 5.0-5.5, in this waste liquid of 40-50 times of volumes of dry yeast, add dry yeast, the dry yeast add-on contains 0.08～0.3% of sugared total amount for the preparation fermented waste fluid, under 33～40 ℃ of temperature, activate 2～3 hours, can obtain fermented bacterium liquid.
Test one:
Detecting waste liquid sugar content is 3%, waste liquid concentrated make its sugared content reach 9%, getting 240ml places little triangular flask to make bacterium culture medium, with in the alkaline fermentation wort sludge in the lactic acid-producing and waste liquid, get pH value 5.4 (with the low mensuration of accurate examination, down together), under 29～30 ℃ of temperature, insert test tube liquid culture bacterial classification saccharomyces carlsbergensis, cultivated 15 hours; With spissated waste liquid 2360ml, transferring pH value with the alkaline fermentation wort sludge in the lactic acid-producing is 6.5, then be cooled to 29-30 ℃, then the cultured bacterial classification of little triangular flask is inserted in the waste liquid, inoculum size 10% (V/V), ferment at constant temperature is 72 hours under 29～30 ℃ of temperature, and the wine degree is 3.4 degree (20 ℃) in the detection karusen, and residual sugar content is 3%.
Test two:
Detecting waste liquid sugar content is 3.94%, getting waste liquid 900ml contains in the triangular flask of 1000ml, make four such samples, regulate pH value 5.5～6.0 with the drystone ashes, selected fermentation scheme: a, little triangular flask substratum adopt bent juice of rice and two kinds of waste liquids (waste liquid is a concentrated solution) respectively; B, fermented waste fluid divide two kinds of sterilization and not sterilizations, and c, fermented waste fluid do not concentrate, and concrete experimental result sees Table one.
Table one
| Sequence number | Substratum | The pre-treatment situation | Leavening temperature | Period hours | Wine degree % | Residual sugar % | Remarks |
| ?1 | The bent juice of rice | Sterilization | 30℃ | ????72 | ?1.8 | ?0.3 | Alcoholic strength is data under 20 ℃ of temperature |
| ?2 | The bent juice of rice | Not sterilization | 30℃ | ????72 | ?1.8 | ?0.35 | |
| ?3 | Waste liquid | Sterilization | 30℃ | ????72 | ?1.9 | ?0.25 | |
| ?4 | Waste liquid | Not sterilization | 30℃ | ????72 | ?1.5 | ?0.45 |
Experimental result shows: the waste liquid sterilization is more higher than the transformation efficiency of not sterilization sugar, and it is close to adopt bent juice of rice and waste liquid to make the substratum effect.
Test three:
Detecting waste liquid sugar content is 3.94%, transfers pH value 5.5～6.0 with the drystone ashes, selectes fermentation scheme: a, adopts waste liquid to make bacterium culture medium; B, waste liquid divide and concentrate and do not concentrate two kinds of situations fermentations, and c, fermentation pre-treatment are unsterilised, also do not add other material, transfer inoculation at once behind the pH value, and concrete experimental result sees Table two.
Table two:
| Sequence number | Fermented liquid | Sugar content % | Volume ml | Leavening temperature | Period hours | Wine degree % | Residual sugar % | Remarks |
| 1 | Concentrate | 11.24 | 700 | 30 ℃ | 80 | 4.3 | 0.35 | The wine degree is data under 20 ℃ of temperature. |
| 2 | Concentrate | 11.24 | 500 | 30 ℃ | 80 | 4.3 | 0.35 | |
| 3 | Do not concentrate | 3.94 | 700 | 30 ℃ | 72 | 1.9 | 0.3 |
Experiment shows: waste liquid concentrates and does not concentrate all energy fermentation finished thoroughly1s, but fermentation period has length.
Test four:
Detecting the waste liquid sugar degree is 3.94%, transfers pH value 5.5～6.0 with the drystone ashes, and selected fermentation scheme: a, employing waste liquid are made bacterium culture medium; B, add nitrogenous source and trace element and do not add two kinds of situations fermentations, it is urea that additive divides other, and dosage is 0.05% of a total reducing sugar, and sal epsom, dosage are 0.05% of total reducing sugar, and dipotassium hydrogen phosphate, dosage are 0.1% of total reducing sugar; C, not sterilization of fermented waste fluid pre-treatment also do not concentrate, inoculation at once behind the accent pH value, and concrete experimental result sees Table three.
Table three
| Sequence number | Add nitrogenous source | Add trace element | Volume ml | Leavening temperature | Period hours | Wine degree % | Residual sugar % | Remarks |
| 1 | Add | Add | ?700 | ?30℃ | ????60 | ?2.3 | ?0.2 | The wine degree is data under 20 ℃ of temperature |
| 2 | Do not add | Do not add | ?700 | ?30℃ | ????60 | ?1.8 | ?0.35 |
Experimental result shows: add nitrogenous source and trace element, than additive effect is not better.
Test five:
Detecting sugared content is 4.3%, getting 800ml places in the plastic tank, transfer pH value 5.5 with the drystone ashes, and add ammonium sulfate 0.15%, sal epsom 0.05%, high temperature resistant active dry yeast kind liquid after inserting activation under 33 ℃ of temperature, the dry yeast dosage is 0.2% (W/W) of fermented liquid total reducing sugar, carries out ferment at constant temperature under 33-35 ℃ of temperature, fermentation time 72 hours, the wine degree is 2.3 degree (20 ℃) in the detection karusen, and residual sugar content is 0.3%.
The activation of high temperature resistant active dry yeast: adopt 2% syrup, its volume is 50 times of dry yeast weight, under 37 ℃ of temperature, and dry yeast water activation 3 hours.
Test six:
Detecting the waste liquid sugar degree is 3.95%, and waste liquid is contained in reactor, and the waste liquid volume is 2200l, transfer pH value 5.4 with the drystone ashes, drystone ashes dosage is 65 liters, adds urea 50 grams, sal epsom 50 grams, under 33 ℃ of temperature, insert the good dry yeast bacterial classification of activation culture, the dry yeast consumption is 250 grams, keeps 33-35 ℃ of temperature fermentations 72 hours, through fractionation by distillation, fermentation liquid ethanol content 2 degree, residual sugar content is 0.25%.
The activation of high temperature resistant active dry yeast: with in the waste liquid and control pH value 5.4, volume is 50 times of dry yeast, under 35 ℃ of temperature, and dry yeast water activation 3 hours.
Above-mentioned used weight is meant corresponding unit weight volume ratio with volume ratio, as kilogram/liter, grams per milliliter.
Advantage of the present invention: (1) can turn waste into wealth, and turns harm into good, and the poor liquid residual sugar resultant from waste liquid behind the extraction alcohol greatly reduces the organic content in the waste liquid below 0.45%; (2) replace the bent juice of rice, wort, syrup etc. to make bacterium culture medium with waste liquid, simplified technology, reduced cost.
Claims (6)
1, a kind of method of from contain sugared waste liquid, extracting alcohol, it is characterized in that: the raw material that is adopted is the waste liquid that yellow ginger or Dioscorea nipponica Mak. Ningpo Yam Rhizome produce saponin, this waste liquid or its concentrated solution are fed neutralization tank, add the alkaline matter neutralization, pH value is controlled between 4～7, under 28～33 ℃ temperature condition, inserting cultured barms ferments, leavening temperature is controlled between 28～40 ℃, fermented 60～80 hours, with the maturing fermentation wine with dregs, carry out fractionation by distillation then, extract alcohol with conventional alcohol producing process.
2, method according to claim 1, it is characterized in that: before inserting cultured barms or after inserting, also can add nitrogenous compound, magnesium salts, phosphoric acid salt, its add-on is: contain sugared total amount in the waste liquid: nitrogen content in the nitrogenous compound: magnesium content in the magnesium salts: phosphorus content in the phosphoric acid salt=100: 0.025～0.03: 0.005～0.008: 0.015～0.03.
3, method according to claim 2 is characterized in that: the nitrogenous source that is replenished and trace element are to contain the ammonium sulfate of sugared total amount 0.15% or 0.05% urea, 0.05% sal epsom and 0.1% hydrogen sulfate dipotassium in the adding waste liquid.
4, according to claim 1,2 or 3 described methods, it is characterized in that: said alkaline matter is the drystone ashes, and dosage is 2～5% of a waste liquid volume, and the control pH value is best between 5～6.
5, according to claim 1,2 or 3 described methods, it is characterized in that: said cultured barms, adopt normal temperature barms saccharomyces carlsbergensis, the volume of its inoculation is 10% of a fermentating liquid volume, inoculation temp is 28～30 ℃, and leavening temperature is 28～32 ℃; Perhaps adopt high temperature resistant active dry yeast, its add-on be fermented liquid contain sugared total amount 0.08～0.3%, inoculation temp is 30～33 ℃, leavening temperature is 30～40 ℃.
6, a kind of cultural method that is exclusively used in claim 1,2 or 3 barms, it is characterized in that: adopt the normal temperature saccharomyces carlsbergensis, it is 12～15% that waste liquid is concentrated to sugar degree, adding the drystone ashes pH value that neutralizes is 5.0～5.5, add the ammonium sulfate of this waste liquid sugar degree 0.15% or 0.05% urea, 0.05% sal epsom and 0.1% dipotassium hydrogen phosphate again, under 28～30 ℃ temperature condition, insert saccharomyces carlsbergensis bacterial classification through the test tube liquid culture, cultivated 15～24 hours, and carried out repeatedly can becoming fermentation kind of liquid after the enlarged culturing; Also can adopt high temperature resistant active dry yeast, in sugar degree is to add the drystone ashes in 3～5% the waste liquid to regulate pH value to 5.0～5.5, substratum as dry yeast, under 33～35 ℃ of temperature, add dry yeast, the add-on of dry yeast contains 0.08～0.3% of sugared total amount for the preparation fermented waste fluid, the above-mentioned waste liquid consumption of making the dry yeast substratum is: its volume equals 40～50: 1 with the ratio that active dry yeast adds weight, under 33～40 ℃ temperature condition, activate 2～3 hours, promptly can be used as fermentation kind of liquid.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94110810 CN1124776A (en) | 1994-01-06 | 1994-01-06 | Process for extracting alcohol from waste liquid in producing saponin with Dioscorea zingiberensis or Dioscorea nipponica |
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| CN 94110810 CN1124776A (en) | 1994-01-06 | 1994-01-06 | Process for extracting alcohol from waste liquid in producing saponin with Dioscorea zingiberensis or Dioscorea nipponica |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064711C (en) * | 1997-05-07 | 2001-04-18 | 昆明市环境科学研究所 | Alcohol extracting method from waste liquid containing sugar and acid |
| CN1307298C (en) * | 2004-08-02 | 2007-03-28 | 赵金忠 | Turmeric wine and its preparation method |
| CN101067143B (en) * | 2007-05-28 | 2010-06-23 | 中国地质大学（武汉） | Process of preparing alcohol with side product sugar liquid from peltate yam saponin production |
| CN101096643B (en) * | 2007-05-28 | 2010-08-25 | 中国地质大学（武汉） | Method for preparing yeast with byproduct sugar solution for production of saponin by turmeric |
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1994
- 1994-01-06 CN CN 94110810 patent/CN1124776A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064711C (en) * | 1997-05-07 | 2001-04-18 | 昆明市环境科学研究所 | Alcohol extracting method from waste liquid containing sugar and acid |
| CN1307298C (en) * | 2004-08-02 | 2007-03-28 | 赵金忠 | Turmeric wine and its preparation method |
| CN101067143B (en) * | 2007-05-28 | 2010-06-23 | 中国地质大学（武汉） | Process of preparing alcohol with side product sugar liquid from peltate yam saponin production |
| CN101096643B (en) * | 2007-05-28 | 2010-08-25 | 中国地质大学（武汉） | Method for preparing yeast with byproduct sugar solution for production of saponin by turmeric |
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