CN2587876Y - Leaching type semicontinuous solid fermentation device - Google Patents
Leaching type semicontinuous solid fermentation device Download PDFInfo
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- CN2587876Y CN2587876Y CN 02289268 CN02289268U CN2587876Y CN 2587876 Y CN2587876 Y CN 2587876Y CN 02289268 CN02289268 CN 02289268 CN 02289268 U CN02289268 U CN 02289268U CN 2587876 Y CN2587876 Y CN 2587876Y
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Abstract
The utility model relates to a leaching type semicontinuous solid fermentation device, which comprises a horizontal type solid fermentation container, a rotary drum type fermentation cylinder, and an internal circulating fan, wherein, the rotary drum type fermentation cylinder is rotatablely and concentrically positioned in the horizontal type solid fermentation container, both ends of the rotary drum type fermentation cylinder are respectively provided with gas distributing plates with a 20% to 30% aperture ratio, and a circulating wind channel is formed between the horizontal type solid fermentation container and the rotary drum type fermentation cylinder. The internal circulating fan is positioned in the horizontal type solid fermentation container and on the front end of the rotary drum type fermentation cylinder. The upper container wall on the rear part of the horizontal type solid fermentation container is provided with a leaching solution inlet, and the lower container wall on the middle part of the horizontal type solid fermentation container is provided with an extracting solution outlet. The diameter ratio of the rotary drum type fermentation cylinder and the horizontal type solid fermentation container is in a range from 0: 7: 1 to 0.9: 1, and the length ratio of the rotary drum type fermentation cylinder and the horizontal type solid fermentation container is in a range from 0.6: 1 to 0.85: 1. The utility model realizes the coupling of semicontinuous solid fermentation with extraction, simplifies operation, improves the utilization rate of equipment, reduces the chance of contamination, and improves the utilization rate of solid substrates.
Description
Technical field
The utility model belongs to the fermentation industry technical field, semicontinuous solid state fermentation of particularly a kind of lixiviate formula and device thereof.
Background technology
(Solid State Fermentation, SSF) technology is that the human use microorganism benefits one of the most ancient technology of oneself to solid state fermentation.Before several thousand, China just utilizes this technology wine brewing and makes various seasonings.So far it is still the main mode of producing traditional food and beverage.Solid state fermentation can also the production zymin, single cell protein, organic acid, alcohol and biotic pesticide etc.At present solid state fermentation is in biological transformation of energy, the processing of solid-state castoff, and aspects such as the production of meta-bolites have also obtained using widely.At present the subject matter that faces of solid state fermentation is, fermentation mode adopts remains batch fermentation, and fermentation period is long, extracts and the lock out operation that ferments, the extraction process complexity, the energy consumption height, the equipment input is many, and operation labour intensity is big, easily microbiological contamination, the solid state substrate utilization ratio is very low, does not meet economic requirement.Therefore, how to realize solid-state continuously fermenting, and realization fermentation and extraction coupling, more effective shortening fermentation period, raising fermentation level, minimizing equipment drop into, and become the hot issue of solid-state fermentation technology research.
And immobilized cell technology to be applied to the report of solid state fermentation be carrier application of expensive, apotrophic mostly, the fixing and fermenting process of cell generally is two relatively independent operating units, the advantage of solid state fermentation raw material cheapness does not demonstrate fully appearance.
Summary of the invention
The fermentation that the purpose of this utility model exists in being to overcome existing solid-state fermentation technology and install separates with extraction element, the solid state substrate utilization ratio is low, plant factor is low, soak defectives such as the body fluid consumption is big, cost height, and provide a kind of lixiviate formula semicontinuous solid-state fermentation installation.
Realize that the utility model purpose technical scheme is as follows:
The semicontinuous solid-state fermentation installation of lixiviate formula that the utility model provides is characterized in that, comprising: a horizontal solid-state fermenter 3, rotary drum fermentation tube 5 and one internal recycling blower fan 1;
Described rotary drum fermentation tube 5 is one to be positioned at one heart within the horizontal solid-state fermenter 3, and rotating revolving horizontal drum formula fermentation tube, this revolving horizontal drum formula fermentation tube 5 two ends are separately installed with gas distribution grid 8, and and horizontal solid-state fermenter 3 inwalls between form circulation air path 4;
Described internal recycling blower fan 1 is positioned at the front end of rotary drum fermentation tube 5 within the horizontal solid-state fermenter 3; Tank skin is provided with the vat liquor inlet on horizontal solid-state fermenter 3 rear portions, this vat liquor ingress is installed the vat liquor that has by-pass valve control 10 and is entered pipe, subordinate's tank skin is provided with the extracting solution relief outlet in the horizontal solid-state fermenter 3, and the extracting solution relief outlet pipe that has by-pass valve control 11 is installed at this extracting solution relief outlet place; Described rotary drum fermentation tube 5 is supported and fixed within the horizontal solid-state fermenter 3 by the support 6 that is embedded on cylindric horizontal solid-state fermenter 3 inwalls.Described rotary drum fermentation tube 5 and horizontal solid-state fermenter 3 are the stainless steel cylinder, and both diameters are than being 0.7-0.9: 1, and length is than being 0.6-0.85: 1; The percentage of open area of described gas distribution grid 8 is 20%-30%; Also be provided with the sterile air inlet mouth on the tank skin on described horizontal solid-state fermenter (3) front portion, belt controling valve (2) inlet pipe is equipped with at the inlet mouth place; Tank skin is provided with venting port under the rear portion, and the exhaust ports dress has by-pass valve control (12) vapor pipe.
The semicontinuous solid state fermentation step of its lixiviate formula is as follows:
A. inoculum fermented material is packed into and carried out being placed on one heart in the rotary drum fermentation tube in the horizontal solid-state fermenter behind the slack tank sterilization and disinfection, build the gas distribution grid at two ends, the horizontal sealing solid-state fermenter, unlatching is positioned at the internal recycling blower fan in rotary drum fermentation tube the place ahead within the horizontal solid-state fermenter, carry out the fermentation of inoculum fermented material, generate tunning;
B. when tunning peaks the phase, open and be positioned at the vat liquor inlet valve that is provided with on the tank skin on the horizontal solid-state fermenter rear portion, in horizontal solid-state fermenter, feed half jar of aseptic vat liquor, tunning in the rotary drum fermentation tube is carried out lixiviate 10-20min, afterwards, rotate rotary drum fermentation tube and make it the Rotate 180 degree, the tunning to second half jar in the rotary drum fermentation tube carries out lixiviate 10-20min again, afterwards, emit vat liquor; Circulation repeats above-mentioned fermentation culture, lixiviate, emits the vat liquor technological process.
The semicontinuous solid-state fermentation installation of lixiviate formula of the present utility model has been realized solid state fermentation and extract phase coupling, has saved and has soaked body fluid, has improved plant factor, has reduced cost, also reduces the chance of microbiological contamination simultaneously, improves the utilization ratio of solid state substrate.
Description of drawings
Accompanying drawing 1 is the semicontinuous solid-state fermentation installation synoptic diagram of lixiviate formula of the present utility model; Wherein:
Recirculation blower 1 sterile air intake valve 2 horizontal solid-state fermenters 3
Circulation air path 4, rotary drum fermentation tube 5 supports 6
Speed change rotating machine 7 gas distribution grids 8 apertures 9
Vat liquor inlet valve 10 vent valves 11 extracting solution outlet valves 12
Embodiment
Accompanying drawing 1 is the semicontinuous solid-state fermentation installation synoptic diagram of lixiviate formula of the present utility model, as shown in Figure 1, the semicontinuous solid-state fermentation installation of lixiviate formula that the utility model that the utility model provides provides, it is characterized in that, comprising: a horizontal solid-state fermenter 3, rotary drum fermentation tube 5 and one internal recycling blower fan 1;
Described rotary drum fermentation tube 5 is one to be positioned at one heart within the horizontal solid-state fermenter 3, and rotating revolving horizontal drum formula fermentation tube, this revolving horizontal drum formula fermentation tube 5 two ends are separately installed with gas distribution grid 8, and and the inwall of horizontal solid-state fermenter 3 between form circulation air path 4;
Described internal recycling blower fan 1 is positioned at the front end of rotary drum fermentation tube 5 within the horizontal solid-state fermenter 3; Tank skin is provided with the vat liquor inlet on described horizontal solid-state fermenter 3 rear portions, this vat liquor ingress is installed the vat liquor that has by-pass valve control 10 and is entered pipe, subordinate's tank skin is provided with the extracting solution relief outlet in the described horizontal solid-state fermenter 3, and the extracting solution relief outlet pipe that has by-pass valve control 11 is installed at this extracting solution relief outlet place; Described rotary drum fermentation tube 5 is supported and fixed within the horizontal solid-state fermenter 3 by the support 6 that is embedded on cylindric horizontal solid-state fermenter 3 inwalls.Described rotary drum fermentation tube 5 and horizontal solid-state fermenter 3 are the stainless steel cylinder, and both diameters are than being 0.7-0.9: 1, and length is than being 0.6-0.85: 1; The percentage of open area of described gas distribution grid 8 is 20%-30%; Tank skin is provided with the sterile air inlet mouth on described horizontal solid-state fermenter (3) front portion, and inlet mouth place dress has by-pass valve control (2) inlet pipe; Tank skin is provided with venting port under the rear portion, and the exhaust ports dress has by-pass valve control (12) vapor pipe.
Embodiment 1
The stainless steel of present embodiment rotary drum fermentation cylindraceous tube 5, the percentage of open area of the gas distribution grid 8 that install at its two ends is 0.2; The diameter of gas distribution grid 8 lip-deep apertures 9 is 2mm; Be used to load the support 6 that rotary drum fermentation tube 5 usefulness of fermentation materials are embedded on cylindric horizontal solid-state fermenter 3 inwalls and be supported and fixed in the horizontal solid-state fermenter 3 circulation air path 4 of formation between the inwall of rotary drum fermentation tube 5 and horizontal solid-state fermenter 3; Rotary drum fermentation tube 5 and horizontal solid-state fermenter 3 are stainless steel cylinders of concentric axle, and both diameter ratio and length ratio were respectively 0.7: 1 and 0.6: 1;
Horizontal solid-state fermenter 3 is outer to connect one by drive shaft rotary drum fermentation tube 5 motor rotating 7, and the place ahead of rotary drums fermentation tube 5 is provided with an internal recycling blower fan 1 of forcing the fermentation materials mass-and heat-transfer in the rotary drum fermentation tube 5 in the horizontal solid-state fermenter 3; The vat liquor inlet tube that vat liquor ingress on horizontal solid-state fermenter 3 rear portions on the tank skin connects connects vat liquor inlet valve 10, and sterile air air inlet place on the front portion on the tank skin connects sterile air intake valve 2; Exhaust ports under the rear portion on the tank skin is connected with vent valve 11, and the extracting solution relief outlet place under the rear portion on the tank skin connects extracting solution outlet valve 12.
Embodiment 2
The stainless steel of present embodiment rotary drum fermentation cylindraceous tube 5 two ends placement percentage of open area is 0.3 gas distribution grid 8; The diameter of gas distribution grid 8 lip-deep apertures 9 is 5mm; Rotary drum fermentation tube 5 and horizontal solid-state fermenter 3 are stainless steel cylinders of concentric axle, and both diameter ratio and length ratio were respectively 0.9: 1 and 0.85: 1.
Other structure is with embodiment 1
Embodiment 3
Adopt the device of embodiment 1, carry out the semicontinuous solid state fermentation of lixiviate formula according to the following steps, concrete steps are as follows:
(1) horizontal solid-state fermenter 3 and rotary drum fermentation tube 5 thereof are carried out the slack tank sterilization and disinfection, after the feeding sterile air is cooled to 40 ℃, (used bacterial classification is with respect to the Penicillium decumbens of fermentation materials quality 10% (Penicilliun.decumbens JUA10) in rotary drum fermentation tube 5 to add inoculum fermented material by aseptic technique, fermentation materials is that component is: the quick-fried fiber material of 90% vapour, 10% wheat bran; The inorganic salt solution proportioning is (NH
4)
2SO
41.5%, MgSO
40.6%, KH
2PO
40.3%; Solid-to-liquid ratio 1: 2.5, the substratum of natural pH), build two ends gas distribution grid 8, horizontal sealing solid-state fermenter 3 is opened internal recycling blower fan 1, carries out solid state fermentation, generates cellulase;
(2) make cellulase after 28 ℃ of bottom fermentations are cultivated 24 hours, open the vat liquor valve, feeding is that 4.8 phosphoric acid buffer (soaking body fluid) is in horizontal solid-state fermenter 3 with respect to 0.02Mol/L, the pH value of horizontal solid-state fermenter 3 volumes 50%, behind the lixiviate 10min, open rotating machine 7, make rotary drum fermentation tube 5 Rotate 180 degree, lixiviate 10min emits vat liquor again; Repeat fermentation culture, lixiviate again, so circulated 15 days;
(3) separate the purification tunning.Obtain the cellulase of 20IU (filter paper enzyme activity)/g (with respect to dry medium) at last.
Embodiment 4
Adopt the device of embodiment 1, carry out the semicontinuous solid state fermentation of lixiviate formula according to the following steps, concrete steps are as follows:
(1) horizontal solid-state fermenter 3 and rotary drum fermentation tube 5 thereof are carried out the slack tank sterilization and disinfection, after the feeding sterile air is cooled to 40 ℃, (bacterial classification that is inserted is with respect to the aspergillus niger (Aspergillus niger AS3.739) of fermentation materials quality 10%, and fermentation materials is that component is: 80% Semen Maydis grit, 19.7% husk, 0.2% lime carbonate, 0.1% ammonium sulfate in rotary drum fermentation tube 5 to add inoculum fermented material by aseptic technique; Solid-to-liquid ratio 1: 2.5, the substratum of natural pH), build two ends gas distribution grid 8, horizontal sealing solid-state fermenter 3 is opened internal recycling blower fan 1, carries out solid state fermentation, generates citric acid;
(2) citric acid was cultivated 16 hours at 28 ℃ of bottom fermentations, open the vat liquor valve, feeding with respect to the sterilized water (soaking body fluid) of horizontal solid-state fermenter 3 volumes 50% in horizontal solid-state fermenter 3, behind the lixiviate 10min, open rotating machine 7, make rotary drum fermentation tube 5 Rotate 180 degree, lixiviate 10min emits vat liquor again; Repeat fermentation culture, lixiviate again, so circulated 10 days.
(3) separate the purification tunning, obtain the citric acid of 7.6% (with respect to the dry medium volume) at last.
Adopt the device of embodiment 2, carry out the semicontinuous solid state fermentation of lixiviate formula according to the following steps, concrete steps are as follows:
(1) horizontal solid-state fermenter 3 and rotary drum fermentation tube 5 thereof are carried out the slack tank sterilization and disinfection, after the feeding sterile air is cooled to 45 ℃, (used bacterial classification is with respect to the Penicillium decumbens of fermentation materials quality 10% (Penicillium decumbens JUA10) in rotary drum fermentation tube 5 to add inoculum fermented material by aseptic technique, fermentation materials is that component is: the quick-fried fiber material of 90% vapour, 10% wheat bran; The inorganic salt solution proportioning is (NH
4)
2SO
41.5%, MgSO
40.6%, KH
2PO
40.3%; Solid-to-liquid ratio 1: 2.5, the substratum of natural pH), build two ends gas distribution grid 8, horizontal sealing solid-state fermenter 3 is opened internal recycling blower fan 1, carries out solid state fermentation, generates cellulase;
(2) cellulase was cultivated 24 hours at 32 ℃ of bottom fermentations, open the vat liquor valve, feeding is that 4.8 phosphoric acid buffer (soaking body fluid) is in horizontal solid-state fermenter 3 with respect to 0.02Mol/L, the pH value of horizontal solid-state fermenter 3 volumes 50%, behind the lixiviate 20min, open rotating machine 7, make rotary drum fermentation tube 5 Rotate 180 degree, lixiviate 20min emits vat liquor again; Repeat fermentation culture, lixiviate again, so circulated 15 days;
(3) separate the purification tunning.Obtain the cellulase of 30IU (filter paper enzyme activity)/g (with respect to dry medium) at last.
Embodiment 6
Adopt the device of embodiment 2, carry out the semicontinuous solid state fermentation of lixiviate formula according to the following steps, concrete steps are as follows:
(1) horizontal solid-state fermenter 3 and rotary drum fermentation tube 5 thereof are carried out the slack tank sterilization and disinfection, after the feeding sterile air is cooled to 50 ℃, (bacterial classification that is inserted is with respect to the aspergillus niger (Aspergillus niger AS3.739) of fermentation materials quality 10%, and fermentation materials is that component is: 80% Semen Maydis grit, 19.7% husk, 0.2% lime carbonate, 0.1% ammonium sulfate in rotary drum fermentation tube 5 to add inoculum fermented material by aseptic technique; Solid-to-liquid ratio 1: 2.5, the substratum of natural pH), build two ends gas distribution grid 8, horizontal sealing solid-state fermenter 3 is opened internal recycling blower fan 1, carries out solid state fermentation, generates citric acid;
(2) citric acid was cultivated 16 hours at 32 ℃ of bottom fermentations, open the vat liquor valve, feeding with respect to the aseptic sterilized water (soaking body fluid) of horizontal solid-state fermenter 3 volumes 50% in horizontal solid-state fermenter 3, behind the lixiviate 20min, open rotating machine 7, make rotary drum fermentation tube 5 Rotate 180 degree, lixiviate 20min emits vat liquor again; Repeat fermentation culture, lixiviate again, so circulated 10 days.
(3) separate the purification tunning, obtain the citric acid of 10.0% (with respect to the dry medium volume) at last.
Claims (5)
1, the semicontinuous solid-state fermentation installation of a kind of lixiviate formula is characterized in that, comprising: a horizontal solid-state fermenter (3), rotary drum fermentation tube (5) and an internal recycling blower fan (1);
Described rotary drum fermentation tube (5) is one to be positioned at one heart within the horizontal solid-state fermenter (3), and rotating revolving horizontal drum formula fermentation tube, this revolving horizontal drum formula fermentation tube (5) two ends are separately installed with gas distribution grid (8), and and the inwall of horizontal solid-state fermenter (3) between form circulation air path (4);
Described internal recycling blower fan (1) is positioned at the front end of rotary drum fermentation tube (5) within the horizontal solid-state fermenter (3);
Tank skin is provided with the vat liquor inlet on described horizontal solid-state fermenter (3) rear portion, its vat liquor ingress is equipped with the vat liquor that has by-pass valve control (10) and enters pipe, subordinate's tank skin is provided with the extracting solution relief outlet in the described horizontal solid-state fermenter (3), and the extracting solution relief outlet pipe that has by-pass valve control (11) is installed at this extracting solution relief outlet place;
As the semicontinuous solid-state fermentation installation of lixiviate formula that claim 1 reached, it is characterized in that 2, described rotary drum fermentation tube (5) is supported and fixed within the horizontal solid-state fermenter (3) by the support (6) that is embedded on cylindric horizontal solid-state fermenter (3) inwall.
3, the semicontinuous solid-state fermentation installation of lixiviate formula as claimed in claim 1 is characterized in that, described rotary drum fermentation tube (5) and horizontal solid-state fermenter (3) they are the stainless steel cylinder, and both diameters are than being 0.7-0.9: 1, and length is than being 0.6-0.85: 1.
4, the semicontinuous solid-state fermentation installation of lixiviate formula as claimed in claim 1 is characterized in that, the percentage of open area of described gas distribution grid (8) is 20%-30%.
5, the semicontinuous solid-state fermentation installation of lixiviate formula as claimed in claim 1 is characterized in that, tank skin is provided with the sterile air inlet mouth on described horizontal solid-state fermenter (3) front portion, and inlet mouth place dress has by-pass valve control (2) inlet pipe; Tank skin is provided with venting port under the rear portion, and the exhaust ports dress has by-pass valve control (12) vapor pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02289268 CN2587876Y (en) | 2002-11-22 | 2002-11-22 | Leaching type semicontinuous solid fermentation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02289268 CN2587876Y (en) | 2002-11-22 | 2002-11-22 | Leaching type semicontinuous solid fermentation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2587876Y true CN2587876Y (en) | 2003-11-26 |
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ID=33748859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02289268 Expired - Lifetime CN2587876Y (en) | 2002-11-22 | 2002-11-22 | Leaching type semicontinuous solid fermentation device |
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| Country | Link |
|---|---|
| CN (1) | CN2587876Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN106070603A (en) * | 2012-06-21 | 2016-11-09 | 黄玧泽 | Home-use Yoghourt fermentation machine |
-
2002
- 2002-11-22 CN CN 02289268 patent/CN2587876Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN102443535B (en) * | 2011-05-10 | 2013-05-01 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN106070603A (en) * | 2012-06-21 | 2016-11-09 | 黄玧泽 | Home-use Yoghourt fermentation machine |
| CN106070603B (en) * | 2012-06-21 | 2019-09-17 | 黄玧泽 | Home-use Yoghourt fermentation machine |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20051207 |
|
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20051207 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |