CN112462050A - Chromatography card shell and chromatography detection card - Google Patents
Chromatography card shell and chromatography detection card Download PDFInfo
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- CN112462050A CN112462050A CN201910883996.8A CN201910883996A CN112462050A CN 112462050 A CN112462050 A CN 112462050A CN 201910883996 A CN201910883996 A CN 201910883996A CN 112462050 A CN112462050 A CN 112462050A
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- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 85
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 238000010790 dilution Methods 0.000 claims abstract description 21
- 239000012895 dilution Substances 0.000 claims abstract description 21
- 239000002274 desiccant Substances 0.000 claims abstract description 20
- 238000001125 extrusion Methods 0.000 claims description 19
- 238000007865 diluting Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 238000003908 quality control method Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003550 marker Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 5
- 239000003085 diluting agent Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 241000194017 Streptococcus Species 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000283707 Capra Species 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a chromatography card shell and a chromatography detection card, wherein the chromatography card shell comprises a card shell cover and a card shell bottom; the shell cover of the card shell is provided with a sample adding hole and an observation window in sequence; the bottom of the card shell is sequentially provided with a dilution liquid tank, a chromatography test paper strip position and a drying agent tank. The chromatography detection card comprises the chromatography card shell and the chromatography test strip. The card shell integrates a dilution liquid tank and a drying agent tank, and solves the problems that the dilution carrying pollution rate is high, the reagent card cannot be placed in a normal wet environment for a long time, and the like.
Description
Technical Field
The invention relates to the field of in-vitro diagnosis, in particular to a chromatography card shell and a chromatography detection card.
Background
With the rapid development of modern technology, automatic detection equipment is widely applied in the medical inspection industry. The automatic detection device can replace most of complex manual operations, has accurate detection results and good consistency, avoids human errors, reduces pollution, and has higher automatic detection speed and higher efficiency.
The immunochromatography technique also presents some problems in automated applications: 1. the full-automatic equipment finishes the processes of sampling, diluting and sample adding by equipment, the dilution is finished in a mixing cup, a sampling head is easy to be polluted, the current common method is to use cleaning liquid and deionized water to clean a needle head and the mixing cup among different samples, and if the cleaning is not clean, the previous sample is brought into the subsequent operation. 2. The immunochromatographic test strip needs to be stored in a dry environment, when a full-automatic instrument is adopted for detection, the reagent card is usually put into a detector in batches after an outer package is removed, and the chromatographic test strip can absorb moisture if the reagent card is in contact with air for a long time.
Disclosure of Invention
In order to solve the problems, a chromatography card shell and a chromatography detection card are developed, wherein the chromatography card shell comprises a card shell cover and a card shell bottom; the shell cover of the card shell is provided with a sample adding hole and an observation window in sequence; the bottom of the card shell is sequentially provided with a dilution liquid tank, a chromatography test paper strip position and a drying agent tank.
In the chromatography card shell, the dilution liquid tank on the bottom of the card shell is positioned at one end of the bottom of the card shell, and the drying agent tank on the bottom of the card shell is positioned at any position from the middle part of the bottom of the card shell to the opposite side of the dilution liquid tank.
In the chromatography card shell, 1 to a plurality of baffles can be arranged in the dilution liquid tank.
The inner surface of the shell cover of the card shell is also provided with a fixing hole, an extrusion strip and an extrusion point respectively; the inner surface of the shell bottom of the clamping shell is provided with a fixing column and a fixing strip; the extrusion strips are respectively positioned on two sides of the observation window on the inner surface of the shell cover.
The fixed columns are respectively positioned at the front part, the middle part and the rear part of the inner surface of the shell bottom; the fixing holes are respectively positioned at the front part, the middle part and the rear part of the inner surface of the shell cover; the fixing column corresponds to the fixing hole in position so as to ensure that the fixing hole and the fixing column can be completely embedded together.
The extrusion strips are respectively positioned at two ends of the observation window.
The fixing strips are respectively positioned at two ends of the chromatographic strip position and used for fixing the chromatographic test strip; the inner side surface of the fixing strip is provided with 1 to a plurality of convex points.
The extrusion points and the sample adding holes are respectively positioned at two ends of the observation window, wherein 2 to a plurality of extrusion points are positioned corresponding to the dryer grooves of the shell bottom, and 1 to a plurality of extrusion points are positioned corresponding to the inner sides of the fixed strips of the shell bottom; the bar code district that the cap surface set up, bar code district and application of sample hole are located the both ends of observation window respectively.
According to the chromatography card shell, the shell cover and the shell bottom are separated or integrated, one end of the integrated shell cover is connected with one end of the shell bottom, or one side of the shell cover is connected with one side of the shell bottom, so that the shell cover and the shell bottom are well embedded; the connection comprises fixed connection or movable connection.
1 to a plurality of sliding grooves are formed in one side of the shell cover and one side of the shell bottom, so that the front and back directions of the shell cover are prevented from being reversed when the separated shell cover is embedded with the shell bottom, and the shell cover and the shell bottom are ensured to be consistent in direction; if the chromatography detection card adopts a full-automatic instrument for detection, the sliding groove on the side surface of the chromatography detection card can be matched with the clamping column on the reagent card box, so that the chromatography detection card slides on the clamping column, and the phenomenon of inclination and turnover when the chromatography detection card is taken out of the card is prevented.
The chromatography card shell is characterized in that the dilution liquid groove is integrated or detachable.
Above-mentioned chromatography card shell, still be equipped with on the casing and dismantle sealed lid, sealed lid covers application of sample hole, observation window.
A chromatography detection card comprises the chromatography card shell and a chromatography test strip, wherein the chromatography test strip is positioned at the position of a chromatography strip at the bottom of the card shell, and two ends of the chromatography test strip are clamped at the inner side of a fixing plate.
The chromatography test strip comprises a bottom plate, wherein a sample pad, a combination pad, a reaction membrane and a water absorption pad are sequentially connected to the bottom plate, and a C quality control line and a T detection line are arranged on the reaction membrane in parallel; depending on the number of detection markers, 1 to several T-lines and C-lines may be provided.
The reaction membrane is provided with a detection area T line and a quality control area C line, wherein the T line is coated with an anti-group B streptococcus antibody, the C line is coated with a goat anti-rabbit polyclonal antibody, and the binding pad is coated with an anti-group B streptococcus antibody marked by colloidal gold.
The baffle in the dilution liquid tank is integrated or can be moved and detached, so that the chromatography detection card is suitable for detection of special items, such as: 1. the sample is treated by two or more diluents and then detected; 2. the sample needs to be diluted by multiple times before detection.
The diluent in the diluent groove is sealed by a sealing film, and the sealing film comprises an aluminum foil film, a plastic film, a rubber film and the like, preferably the aluminum foil film.
The extrusion points on the shell cover correspond to the chromatography test paper strips and the drying agents on the shell bottom respectively, so that the fixed reagent strips and the drying agents are prevented from being shifted and shaken in the transportation and use processes.
The shell bottom is provided with a chromatography test strip position, and the chromatography test strip is placed on the chromatography test strip position on the shell bottom and is embedded between the fixing strips at the two ends.
Technical effects
1. According to the chromatography card shell, the dilution liquid tank is connected to the chromatography card shell, the dilution and sample adding processes are carried out in the dilution liquid tank on the chromatography detection card, and each chromatography card is provided with one dilution liquid tank, so that the influence on the detection result caused by bringing the previous sample into the subsequent operation due to the unclean cleaning is avoided, and the probability of wrong adding of the dilution liquid can be effectively reduced; in addition, the chromatography card shell is connected with a diluent tank, and a diluent container does not need to be additionally equipped, so that the production cost is reduced.
2. According to the chromatography card shell, the drying agent groove is formed in the shell bottom, when the chromatography detection card is assembled, the drying agent is filled into the drying agent groove, and after an outer package of the chromatography detection card is opened, the drying agent is filled in the card shell, so that the chromatography test strip can keep a drying state for a long time in a normal wet environment, the chromatography test strip is prevented from affecting the chromatography effect due to moisture absorption, and the detection error is reduced.
Drawings
FIG. 1: is a schematic structural diagram of the outer surface of the shell cover of the chromatography card shell.
FIG. 2: is a schematic structural diagram of the inner surface of the shell cover of the chromatography card shell.
FIG. 3: the structure of the inner surface of the shell bottom of the chromatography card is shown schematically, wherein the diluting fluid tank is integrated.
FIG. 4: the inner surface of the shell cover of the chromatography card shell is in a schematic structure, wherein the diluting fluid groove is detachable.
FIG. 5: is a structural schematic diagram of the connection of the chromatography shell cover and the chromatography shell bottom.
FIG. 6: is a schematic diagram of a chromatographic test strip.
Description of reference numerals: 101-shell cover, 102-shell cover, 201-shell bottom, 202-shell bottom, 6-observation window, 7-sample adding hole, 8-extrusion strip, 9-extrusion point, 10-fixed hole, 11-fixed column, 12-dilution liquid tank, 13-drying agent tank, 14-salient point, 15-fixed strip, 16-sliding groove, 17-chromatography test strip position, 18-bar code area, 21-sample pad, 22-combination pad, 23-reaction membrane, 24-water absorption pad, 25-bottom plate, T-detection area T line and C-quality control area C line.
Detailed Description
The invention is further described with reference to the following examples.
Example 1
A chromatography card shell comprises a card shell cover 101 and a card shell bottom 201; the shell cover of the card shell is sequentially provided with a sample adding hole 7 and an observation window 6; the bottom of the card shell is sequentially provided with a dilution liquid tank 12, a chromatography test strip position 17 and a drying agent tank 13; the inner surface of the shell cover is also respectively provided with a fixing hole 10; the inner surface of the shell bottom is provided with a fixed column 11 and a fixed strip 15.
The diluting liquid tank and the drying agent tank on the bottom of the clamping shell are respectively positioned at two ends of the bottom of the clamping shell; the dilution liquid tank is integrated.
Example 2
A chromatography card shell comprises a card shell cover 101 and a card shell bottom 201; the shell cover of the card shell is sequentially provided with a sample adding hole 7 and an observation window 6; the bottom of the card shell is sequentially provided with a dilution liquid tank 12, a chromatography test strip position 17 and a drying agent tank 13; the inner surface of the shell cover is also provided with a fixing hole 10, an extrusion strip 8 and an extrusion point 9 respectively; the inner surface of the shell bottom is provided with a fixed column 11 and a fixed strip 15.
The diluting liquid tank and the drying agent tank on the bottom of the clamping shell are respectively positioned at two ends of the bottom of the clamping shell; the diluting liquid groove is of a separated type.
Example 3
A chromatography card shell comprises a card shell cover 101, a card shell bottom 202; the shell cover of the card shell is sequentially provided with a sample adding hole 7 and an observation window 6; the bottom of the card shell is sequentially provided with a dilution liquid tank 12, a chromatography test strip position 17 and a drying agent tank 13; the inner surface of the shell cover is also provided with a fixing hole 10, an extrusion strip 8 and an extrusion point 9 respectively; the inner surface of the shell bottom is provided with a fixed column and a fixed strip.
The diluting liquid tank and the drying agent tank on the bottom of the clamping shell are respectively positioned at two ends of the bottom of the clamping shell; and 2 sliding grooves are respectively formed in one side of the shell cover and one side of the shell bottom, and the diluting liquid groove is integrated.
Example 4
A chromatography detection card comprises the chromatography card shell and a chromatography test strip in embodiment 3, wherein the chromatography test strip comprises a bottom plate 25, a sample pad 21, a binding pad 22, a reaction membrane 23 and a water absorption pad 24 are sequentially connected on the bottom plate, the reaction membrane is provided with a detection zone T line and a quality control zone C line, the T line is coated with an anti-group B streptococcus antibody, the C line is coated with an anti-rabbit polyclonal antibody, and the binding pad is coated with an anti-group B streptococcus antibody marked by colloidal gold.
The diluting liquid tank and the drying agent tank on the bottom of the clamping shell are respectively positioned at two ends of the bottom of the clamping shell; and 2 sliding grooves are respectively formed in one side of the shell cover and one side of the shell bottom, and the diluting liquid groove is integrated.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the true spirit and scope of the present invention.
Claims (10)
1. A chromatography card shell comprises a card shell cover and a card shell bottom; the shell cover of the card shell is provided with a sample adding hole and an observation window in sequence; the bottom of the card shell is sequentially provided with a dilution liquid tank, a chromatography test paper strip position and a drying agent tank.
2. A chromatography card casing according to claim 1, characterized in that: the diluting liquid tank on the bottom of the clamping shell is positioned at one end of the bottom of the clamping shell, the drying agent tank on the bottom of the clamping shell is positioned at any position from the middle part of the bottom of the clamping shell to the opposite side of the diluting liquid tank, 1 to a plurality of baffles can be arranged in the diluting liquid tank, and the diluting liquid tank is integrated or detachable.
3. A chromatography card casing according to claim 1 or 2, characterized in that: the inner surface of the shell cover of the card shell is provided with a fixing hole, an extrusion strip and an extrusion point; the inner surface of the bottom of the clamping shell is also provided with a fixed column and a fixed strip respectively.
4. A chromatography card casing according to claim 3, characterized in that: the fixed columns are respectively positioned at the front part, the middle part and the rear part of the inner surface of the shell bottom; the fixing holes are respectively positioned at the front part, the middle part and the rear part of the inner surface of the shell cover; the fixing column corresponds to the fixing hole in position; the fixing strips are respectively positioned at two ends of the chromatography strip position, and two parallel fixing strips are respectively arranged at the two ends; the inner side surface of the fixing strip is provided with 1 to a plurality of convex points.
5. A chromatography card casing according to claim 3, characterized in that: the extrusion points and the sample adding holes are respectively positioned at two ends of the observation window, wherein 2 to a plurality of extrusion points are positioned corresponding to the dryer grooves of the shell bottom, and 2 to a plurality of extrusion points are positioned corresponding to the inner sides of the fixing strips of the shell bottom; the surface of the shell cover is provided with a bar code area, and the bar code area and the sample adding hole are respectively positioned at two ends of the observation window.
6. A chromatography card casing according to claim 1 or 2, characterized in that: the shell cover and the shell bottom are separated or integrated, one end of the integrated shell cover is connected with one end of the shell bottom, or one side of the shell cover is connected with one side of the shell bottom, and the connection comprises fixed connection or movable connection.
7. A chromatography card casing according to any of claims 1-6, characterized in that: and 1 to a plurality of sliding grooves are respectively arranged on one side of the shell cover and one side of the shell bottom, wherein the positions of the sliding grooves of the shell cover and the shell bottom correspond to each other.
8. A chromatography card casing according to claim 7, characterized in that: above-mentioned chromatography card shell, still be equipped with on the casing and dismantle sealed lid, sealed lid covers application of sample hole, observation window.
9. A chromatography detection card comprises the chromatography card shell and the chromatography test paper strip of any one of the items 1-8, and is characterized in that: the chromatography test strip is positioned at the chromatography strip position at the bottom of the card shell, two ends of the chromatography test strip are clamped at the inner side of the fixing plate, the chromatography test strip comprises a bottom plate, a sample pad, a combination pad, a reaction membrane and a water absorption pad are sequentially connected onto the bottom plate, and a C quality control line and a T detection line are arranged on the reaction membrane in parallel.
10. The chromatography detection card of claim 9, wherein: the conjugate pad is coated with an antibody labeled with a marker, the T line is coated with an antibody matched with the antibody on the conjugate pad, and the C line is coated with a polyclonal antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910883996.8A CN112462050A (en) | 2019-09-06 | 2019-09-06 | Chromatography card shell and chromatography detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910883996.8A CN112462050A (en) | 2019-09-06 | 2019-09-06 | Chromatography card shell and chromatography detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112462050A true CN112462050A (en) | 2021-03-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910883996.8A Pending CN112462050A (en) | 2019-09-06 | 2019-09-06 | Chromatography card shell and chromatography detection card |
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| CN (1) | CN112462050A (en) |
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