CN112451460A - Extraction method of crocus sativus extract and stretch mark removing cream containing extract - Google Patents
Extraction method of crocus sativus extract and stretch mark removing cream containing extract Download PDFInfo
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Abstract
The invention discloses a method for extracting a crocus extract, which mainly comprises the steps of pre-treating, breaking the wall of crocus, extracting crocin, purifying crocin, extracting crocin polysaccharide, decoloring crocin polysaccharide extract, purifying crocin polysaccharide and the like. The invention also discloses stretch mark cream containing the extract and a preparation method thereof. The extraction method of the crocus sativus extract and the stretch mark removing cream containing the extract have the characteristics of high extraction efficiency, high extraction temperature and good activity of the extract.
Description
Technical Field
The invention relates to the technical field of cosmetic raw materials, in particular to a method for extracting crocus sativus extract and striae gravidarum removing cream containing the extract.
Background
The striae gravidarum is mainly formed by that the striae gravidarum is influenced by hormone during pregnancy, the swelling of the abdomen makes the elastic fiber and the collagen fiber of the skin damaged or broken in different degrees due to the external force traction, the skin becomes thin and thin, and the skin of the abdominal wall can have pink or purple wavy patterns with different widths and lengths. After delivery, these patterns gradually disappear, leaving white or silvery-white, shiny scar lines, i.e. stretch marks. Much research has been done around the cause of such trauma, leading to more complex conclusions.
Since 1959, a study focused on the correlation between striae gravidarum and adrenal cortex hyperplasia, explained the "striae factor striatactor", but also suggested that "striae formation is not directly related to skin stretching" and that olive oil massage does not prevent striae gravidarum from occurring. In 1972, however, Davey found that olive oil massage had a surprising effect on preventing striae gravidarum. Novel treatments such as castor oil, seaweed ingredients, topical application of glycolic and tartaric acids and creams containing multiple ingredients, topical application of 0.1% retinoic acid cream to treat early striae distensae, and treatment of old striae distensae with two regimens (20% glycolic acid, 10% levovitamin C,2% zinc sulfate and 0.5% tyrosine; 20% glycolic acid, 0.1% retinoic acid ointment) concluded that the treatment regimen of glycolic acid in combination with retinoic acid-lactic acid ointment increased the elastic fiber content in the striae distensae, and had some effect on improving the appearance of striae distensae. Topical application of tretinoin acid cream significantly improved the appearance of early striae distensae (subjects not including pregnant and parturient women), but this effect could not be generalized to older striae gravidarum. Studies with tretinoin mixed with glycolic acid and vitamin C mixed with glycolic acid concluded that both treatment regimens improved the appearance of mature stretch marks. In recent years, laser therapy (585nm pulse dye laser) has been developed, and a high-intensity, multi-spectral, incoherent light source with a wavelength range of 500nm-1200nm has been developed, and these characteristics make skin tissues selectively absorb intense pulsed light with different wavelengths and produce a photothermolysis effect relatively prominent. While the opposite rf technology is more efficient and effective in combination with laser technology.
In clinic, striae gravidarum is repaired in three ways, namely, proper anti-inflammation, oxidation resistance, regeneration and repair. The comprehensive treatment of repairing components and physical therapy is the mainstream in the market at present, and specifically comprises photon pigment camouflage, micro-grinding, multi-pole radio frequency treatment and dot matrix laser operation. The stretch mark cream is produced by providing a repairing component to cooperate with physical therapy or treating relatively shallow stretch marks in a short time.
At present, the application of the crocus extract in the field of cosmetics becomes a hot point in the industry, and the main methods of the crocus extract at present comprise a solvent extraction method, a microwave extraction method, a supercritical fluid extraction method, an ultrasonic auxiliary extraction method and the like.
Taking the ultrasonic extraction method as an example, the ultrasonic extraction utilizes the strong vibration and cavitation effect generated by ultrasonic to accelerate the release, diffusion and dissolution of the substances in plant cells into a solvent, and simultaneously can keep the structure and biological activity of the extracted substances unchanged. The principle of ultrasonic extraction is mainly a physical process, and is a relatively new extraction method which is gradually emphasized in recent years. For most components, the ultrasonic extraction method can greatly shorten the extraction time compared with the conventional solvent extraction, consumes less solvent and has high extraction efficiency.
In the process of the ultrasonic extraction process, the selection and concentration of a solvent, the feed-liquid ratio, the extraction temperature and the extraction time directly influence the extraction rate. Ling Zhou et al extract fructus Schisandrae chinensis by ultrasonic extraction, mainly studied the influence factor of ultrasonic extraction rate, the experimental study has shown that the extraction rate increases with the temperature rise, and increases with the power increase. The Hong Van Le and the like extract vitamin E and phenolic compounds in the cherries by utilizing ultrasonic waves, and mainly compare the difference between the ultrasonic extraction method and the enzyme extraction method in extraction time and extraction rate, and experimental results show that the ultrasonic extraction method is shortened by 6 times in extraction time compared with the enzyme extraction method, and the extraction rate of the ultrasonic extraction is 2-3 times of that of the enzyme extraction method. A method for extracting chlorophyll from fresh bamboo leaves by ultrasonic waves in the Stachys province and the like uses a spectrophotometer to quantitatively determine the content of the extracted chlorophyll. The results show that: compared with the common organic solvent extraction method, the ultrasonic extraction method has the advantages of high extraction rate, high speed and high efficiency, and can be used for extracting at room temperature without heating, thereby saving energy.
However, in the extraction process of crocus sativus, the increase of temperature and power can cause the rapid degradation of polysaccharide into monosaccharide or small molecular reducing sugar while improving the extraction rate, and reduce the activity of polysaccharide, thereby influencing the function of crocus sativus extract as the effective component of cosmetics.
Disclosure of Invention
The invention aims to provide an extraction method of a crocus sativus extract and stretch mark removing cream containing the extract, and the crocus sativus extract has the characteristics of high extraction efficiency, high extraction temperature and good activity of the extract.
The invention can be realized by the following technical scheme:
the invention discloses an extraction method of a crocus sativus extract, which comprises the following steps:
(1) pretreatment of raw materials: cleaning crocus sativus, removing dust and particulate impurities on the surface, cutting into pieces, and oven drying at 45-60 deg.C;
(2) wall breaking treatment of crocus sativus: pulverizing pretreated crocus sativus, adding 75-85% ethanol solution and crocus sativus according to the liquid-material weight ratio of 40-60:1, pulverizing, and breaking the wall in a wall breaking machine to obtain crocus sativus wall-broken liquid;
(3) extracting and processing crocin: ultrasonically extracting the wall-broken liquid of crocus sativus at 50-65 ℃ for 0.5-2 h, centrifuging to obtain crocin saponin extract and crocin residue, and spin-drying the crocin saponin extract with a rotary evaporator to obtain crocin saponin crude product;
(4) and (3) purifying crocin: diluting the crocin crude product with a proper amount of distilled water, adding the diluted crocin crude product into prepared adsorption resin for adsorption, eluting with distilled water, then eluting with 70-85% ethanol until no saponin flows out of the eluent, namely, completely eluting, collecting ethanol eluent, spin-drying with a rotary evaporator, and drying with a reduced pressure dryer to constant weight to obtain the crocin finished product:
(5) extracting and treating crocus sativus polysaccharide: adding distilled water 15-25 times the weight of the crocus sativus residue in the step (3), performing reflux extraction at 60-80 ℃ for 1-3 times after wall breaking treatment, extracting for 2-4.5h each time, and mixing water extract to obtain crocus sativus polysaccharide extract;
(6) decoloring the crocus polysaccharide extracting solution: adding a decolorizing agent 0.1-0.5 times of the weight of the crocus polysaccharide extract into the crocus polysaccharide extract, stirring for 20-40min at the rotating speed of 200-400r/min for decolorizing for 1-2 times, and filtering to obtain decolorized crocus polysaccharide extract;
(7) and (3) purifying crocus polysaccharide: concentrating the decolorized crocus sativus polysaccharide extract under reduced pressure, concentrating by 20-30 times, adding the rest extract into adsorption resin prepared in advance for adsorption, eluting with distilled water until no polysaccharide flows out of the eluate, collecting the eluate, and drying the eluate in a spray dryer to obtain crocus sativus polysaccharide.
In the invention, the uniformity of the liquid material system is ensured by adopting the wall breaking process for treatment, the possibility is provided for effectively reducing the extraction time, the possibility is provided for accelerating the extraction finishing rate due to the uniformity of the system, and the extraction efficiency is effectively improved; when the extraction rate is increased, the extraction rate is ensured without excessively increasing the ultrasonic power and the extraction temperature, the degradation and damage of the polysaccharide caused by excessively high power or excessively high temperature are effectively avoided, and the activity of the extract is effectively maintained.
Further, the extraction treatment of crocin in step (3) is continuous ultrasonic treatment, an ultrasonic extraction pipeline is arranged in the ultrasonic cavity, a continuous discharge pipe is arranged at one end of the ultrasonic extraction pipeline, and a double-spiral air inlet pipe for inputting hydraulic air and a liquid inlet pipe for inputting crocin wall breaking liquid are arranged at the other end of the ultrasonic extraction pipeline.
According to the technical scheme, the double-helix gas inlet pipe and the double-helix liquid inlet pipe are adopted to form gas-liquid mixed fluid, intense vortex diffusion is generated inside liquid material cells under the action of ultrasonic stirring and vibration, the concentration difference inside and outside the cells is increased, the mass transfer rate of solute is accelerated, and the extraction rate is improved. The air inlet pipe is arranged, high-pressure air is sprayed out, and when bubbles float upwards, three-phase fluidization of gas, liquid and solid is formed to flow in the ultrasonic extraction pipeline, so that the condition of uneven extraction caused by the existence of a stagnant area is effectively overcome, and the extraction efficiency is effectively ensured. Meanwhile, the improvement of the extraction efficiency avoids the degradation damage of polysaccharide caused by overhigh temperature or overlarge power by improving the temperature and increasing the ultrasonic power.
Further, intake pipe and feed liquor pipe double helix form stretch into to drawing the pipeline inner chamber, and the inner chamber part that the intake pipe stretched into and draws the pipeline is equipped with the fumarole, and the portion that the feed liquor pipe stretched into and draws the pipeline inner chamber is equipped with the hydrojet pipe.
Further, the aperture of the air injection hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole. The aperture of the liquid spraying hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole. By setting gradient change of the aperture, a vortex diffusion gradient is formed, concentration difference is accelerated, and extraction rate is improved.
Furthermore, the continuous discharging pipe is a spiral pipeline, and the tail end of the continuous discharging pipe is provided with an adjusting valve for flow control. Through the regulating valve, the extraction time of liquid in the ultrasonic extraction pipeline can be controlled and regulated, and the extraction efficiency is ensured.
Another aspect of the invention is to protect stretch mark removing cream, wherein the stretch mark removing cream comprises the crocus sativus extract. The crocus extract is crocus saponin crude product, crocus saponin finished product, crocus polysaccharide extract and/or crocus polysaccharide.
Preferably, the stretch mark removing cream comprises the following components in parts by weight:
phase A: 35-42 parts of water, 4-6 parts of glycerol, 0.01-0.03 part of EDTA-2Na and 0.10-0.30 part of allantoin;
phase B: 0.40-0.60 part of cetyl alcohol, 0.40-0.60 part of glyceryl stearate, 0.40-0.60 part of PEG-75 stearate, 0.78-0.60 part of ceteth-200.40, 1-3 parts of cetearyl alcohol, 0.40-0.60 part of cetearyl glucoside, 0.15-0.25 part of sodium stearyl glutamate, 4-8 parts of isononyl isononanoate, 0.15-0.25 part of tocopherol acetate and 1.5-2.5 parts of olive oil;
and C phase: 0.60-1.0 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.15-0.25 part of C18-21 alkyl and 0.32-0.25 part of tridecyl alcohol polyether-60.15;
phase E: 1.5-2.5 parts of butanediol, 0.40-0.60 part of jasminolone and 0.40-0.60 part of 1,2 hexanediol;
and (3) phase F: 13-17 parts of vital repair peptide, 4-6 parts of glycerol glucoside, 1.5-2.5 parts of isosorbide dimethyl ether, 8-12 parts of hydroxyprolinol CN, 3-7 parts of crocus sativus extract and 1.5-2.5 parts of asiaticoside;
phase H: 0.003 to 0.006 portion of essence.
Preferably, the stretch mark removing cream comprises the following components in parts by weight:
phase A: 38.075 parts of water, 5.00 parts of glycerol, 0.02 part of EDTA-2Na and 0.20 part of allantoin;
phase B: 0.50 part of cetyl alcohol, 0.50 part of glycerol stearate, 0.50 part of PEG-75 stearate, 200.50 parts of ceteth-200.50 parts, 2.00 parts of cetearyl alcohol, 0.50 part of cetearyl glucoside, 0.20 part of sodium stearyl glutamate, 6.00 parts of isononyl isononanoate, 0.20 part of tocopherol acetate and 2.00 parts of olive oil;
and C phase: 0.80 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.20 part of C18-21 alkyl and 60.20 parts of tridecyl polyether;
phase E: 2.00 parts of butanediol, 0.50 part of jasminon and 0.50 part of 1,2 hexanediol;
and (3) phase F: 15.00 parts of ultimate repair peptide, 5.00 parts of glycerol glucoside, 2.00 parts of isosorbide dimethyl ether, 10.00 parts of hydroxyl prolinol CN, 5.00 parts of crocus sativus extract and 2.00 parts of asiaticoside;
phase H: 0.005 part of essence.
In the invention, the uniformity of the liquid material system is ensured by adopting the wall breaking process for treatment, the possibility is provided for effectively reducing the extraction time, the possibility is provided for accelerating the extraction finishing rate due to the uniformity of the system, and the extraction efficiency is effectively improved; when the extraction rate is increased, the extraction rate is ensured without excessively increasing the ultrasonic power and the extraction temperature, the degradation and damage of the polysaccharide caused by excessively high power or excessively high temperature are effectively avoided, and the activity of the extract is effectively maintained.
Another aspect of the present invention is to provide a method for preparing the stretch mark removing cream, comprising the following steps: heating A, B phase to 80-90 deg.C, stirring, homogenizing, adding oil slowly into phase B and phase A, emulsifying, homogenizing for 2-5 min; adding C, D phase, homogenizing for 2-5 min, cooling, defoaming to 40-50 deg.C, adding E phase, dissolving, sequentially adding F, H phases, and homogenizing.
The invention relates to a method for extracting crocus sativus extract and stretch mark removing cream containing the extract, which have the following beneficial effects:
according to the technical scheme, the double-helix gas inlet pipe and the double-helix liquid inlet pipe are adopted to form gas-liquid mixed fluid, intense vortex diffusion is generated inside liquid material cells under the action of ultrasonic stirring and vibration, the concentration difference inside and outside the cells is increased, the mass transfer rate of solute is accelerated, and the extraction rate is improved. The air inlet pipe is arranged, high-pressure air is sprayed out, and when bubbles float upwards, three-phase fluidization of gas, liquid and solid is formed to flow in the ultrasonic extraction pipeline, so that the condition of uneven extraction caused by the existence of a stagnant area is effectively overcome, and the extraction efficiency is effectively ensured.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the following provides a detailed description of the product of the present invention with reference to the examples.
Example 1
The invention discloses an extraction method of a crocus sativus extract, which comprises the following steps:
(1) pretreatment of raw materials: cleaning stigma croci Sativi, removing dust and particulate impurities on surface, cutting into pieces, and oven drying at 45-60 deg.C. By low-temperature drying, the degradation damage of polysaccharide caused by overhigh temperature is avoided, and the influence on the drying efficiency caused by overlow temperature (such as lower than 45 ℃) is also noticed.
(2) Wall breaking treatment of crocus sativus: pulverizing pretreated crocus sativus, adding 75-85% ethanol solution and crocus sativus according to the liquid-material weight ratio of 40-60:1, pulverizing, and breaking the wall in a wall breaking machine to obtain crocus sativus wall-broken liquid. The liquid-material ratio is set, generally speaking, the extraction rate can be increased along with the increase of the liquid-material ratio, but in practice, the extraction rate tends to increase first and then decrease, mainly because the increase of the liquid-material ratio when the polysaccharide is added to the liquid material to be basically extracted completely can cause impurities to enter the leaching solution, so that the viscosity of the leaching solution is increased, the diffusion speed is reduced, and the polysaccharide is not easy to release. In addition, when the solvent amount is too large, the ultrasonic radiation is absorbed by the solvent more, and the action on the material is relatively reduced, so that the separation of the polysaccharide is influenced, and the extraction of the polysaccharide is not facilitated when the liquid-material ratio is too large or too small.
(3) Extracting and processing crocin: ultrasonic extracting the wall-broken liquid of crocus sativus at 50-65 deg.C for 0.5-2 h, centrifuging to obtain crocin saponin extract and crocin residue, and spin-drying the crocin saponin extract with rotary evaporator to obtain crocin saponin crude product. As for the extraction temperature, generally speaking, with the increase of the temperature, the movement among solvent molecules is increased, the diffusion is promoted, so that the increase of the extraction rate is facilitated, but the extraction rate tends to be reduced when the temperature is too high, and partial polysaccharide is damaged and degraded as a result of the synergistic effect of the high temperature and the ultrasonic crushing, and the activity of the polysaccharide is reduced. After wall breaking treatment, the liquid-material ratio is properly controlled. The extraction temperature is not required to be too high, the control at 50-65 ℃ is more suitable, and the defect that the traditional extraction method is up to 90 ℃ is effectively avoided. For the extraction time, the extraction rate of the polysaccharide is slowed down after reaching the maximum value along with the increase of the ultrasonic time, and the intense vortex diffusion is generated inside the material cells under the action of ultrasonic stirring and vibration, so that the concentration difference inside and outside the cells is increased, the mass transfer rate of solute is accelerated, and the extraction rate is improved; when a certain critical point is reached, the ultrasonic time is prolonged, the extraction rate of the polysaccharide is reduced, and the polysaccharide molecules are broken (2) due to the long-time sample treatment mainly under the action of ultrasonic mechanical shearing, so that the extraction rate of the polysaccharide is influenced.
(4) And (3) purifying crocin: diluting the crocin crude product with a proper amount of distilled water, adding the diluted crocin crude product into prepared adsorption resin for adsorption, eluting with distilled water, eluting with 70-85% ethanol until no saponin flows out of the eluent, collecting the ethanol eluent, spin-drying with a rotary evaporator, and drying with a reduced pressure dryer to constant weight to obtain the crocin finished product.
(5) Extracting and treating crocus sativus polysaccharide: adding distilled water 15-25 times the weight of the crocus sativus residue in the step (3), performing wall breaking treatment, performing reflux extraction at 60-80 ℃ for 1-3 times, extracting for 2-4.5h each time, and mixing water extract to obtain crocus sativus polysaccharide extract.
(6) Decoloring the crocus polysaccharide extracting solution: adding 0.1-0.5 times of decolorizing agent into the stigma croci Sativi polysaccharide extractive solution, stirring at a rotation speed of 200 and 400r/min for 20-40min for decolorizing for 1-2 times, and filtering to obtain decolorized stigma croci Sativi polysaccharide extractive solution.
(7) And (3) purifying crocus polysaccharide: concentrating the decolorized crocus sativus polysaccharide extract under reduced pressure, concentrating by 20-30 times, adding the rest extract into adsorption resin prepared in advance for adsorption, eluting with distilled water until no polysaccharide flows out of the eluate, collecting the eluate, and drying the eluate in a spray dryer to obtain crocus sativus polysaccharide.
Further, the extraction treatment of crocin in step (3) is continuous ultrasonic treatment, an ultrasonic extraction pipeline is arranged in the ultrasonic cavity, a continuous discharge pipe is arranged at one end of the ultrasonic extraction pipeline, and a double-spiral air inlet pipe for inputting hydraulic air and a liquid inlet pipe for inputting crocin wall breaking liquid are arranged at the other end of the ultrasonic extraction pipeline.
Further, intake pipe and feed liquor pipe double helix form stretch into to drawing the pipeline inner chamber, and the inner chamber part that the intake pipe stretched into and draws the pipeline is equipped with the fumarole, and the portion that the feed liquor pipe stretched into and draws the pipeline inner chamber is equipped with the hydrojet pipe.
Further, the aperture of the air injection hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole. The aperture of the liquid spraying hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole.
Furthermore, the continuous discharging pipe is a spiral pipeline, and the tail end of the continuous discharging pipe is provided with an adjusting valve for flow control.
In the above extraction process, the preparation method of the adsorbent resin comprises the following steps:
(a) preparing a polyaspartic acid saturated solution by using deionized water at room temperature, dropwise adding a 10% sulfuric acid solution to adjust the pH value to 4-5, adding hydroxypropyl methyl cellulose, adding water, heating and raising the temperature, keeping the temperature and stirring for 2-5 hours when the hydroxypropyl methyl cellulose is completely dissolved after heating to a reflux state, and obtaining a reaction product for later use;
(b) uniformly mixing a styrene monomer, an acrylate monomer and the reaction product monomer in the step (a) to obtain an oil phase mixture, adding an initiator and a pore-forming agent into the oil phase mixture, and stirring to fully dissolve the initiator and the pore-forming agent to obtain a mixture;
(c) adding sodium pyrophosphate, polyvinyl alcohol and an auxiliary agent into water which is 2-5 times the weight of the mixture in the step (b), starting stirring at the stirring speed of 150-300r/min, adding the mixture in the step (b) into water which is continuously stirred, continuing stirring, heating a reaction system to 70-85 ℃ after the mixture forms uniform liquid drops in the water, carrying out suspension polymerization reaction for 20-25h, raising the temperature to 85-95 ℃ after the reaction is finished, and carrying out curing reaction for 15-20h to obtain the adsorption resin.
Preferably, the mass ratio of the polyaspartic acid to the hydroxypropyl methylcellulose in the step (a) is 10-15: 5-10.
Preferably, the weight ratio of the styrene monomer, the acrylate monomer and the reaction product monomer in the step (a) is 15-40:15-25: 35-70.
Preferably, the weight ratio of the total weight of the monomers in the step (b) to the initiator to the porogen is 90-100: 0.5-1.5: 50-140.
Preferably, the weight ratio of the water, the sodium pyrophosphate, the polyvinyl alcohol and the auxiliary agent in the step (c) is 80-100:0.5-2:1-2.5: 2-5.
Preferably, the auxiliary agent is a mixture of potassium sorbate, disodium edetate and magnesium stearate in a weight ratio of 10-15:5-10: 2-8.
Preferably, in step (b), the initiator is dimethyl azo-diisobutyrate, and the pore-forming agent is a mixture of polyvinylpyrrolidone and talcum powder in a weight ratio of 15-25: 5-10.
Further, the preparation method of the decolorizing agent comprises the steps of roasting and activating diatomite, bentonite and attapulgite for 2-3 h at 70-95 ℃, adding sodium dodecyl sulfate and water, stirring uniformly to dissolve and disperse the diatomite, the bentonite and the attapulgite into uniform aqueous solution, adding 0.1-2.0mol/L sodium hydroxide solution to ensure that the pH value is 8-10, carrying out ultrasonic treatment for 0.5-1h, then placing the solution in a closed container, heating to 100 ℃ and 150 ℃ for hydrothermal reaction for 4-6h, cooling to room temperature after the reaction is finished, filtering to obtain precipitate, adding 0.2-0.4 mol/L sodium alginate solution with the weight being 2-3 times of the weight of the precipitate, adding polysorbate with the weight being 1-2 times of the weight of the sodium alginate solution, carrying out ultrasonic dispersion for 0.5-1h, carrying out constant temperature reaction for 2-4 h at 45-60 ℃, then sending into a spray dryer for drying, and then crushing by an ultrafine crusher to obtain the product.
Preferably, the weight ratio of the diatomite, the bentonite, the attapulgite, the sodium dodecyl sulfate and the water is 50-80: 40-60: 60-90:1-4: 80-120.
Example 2
Another aspect of the invention is to protect stretch mark removing cream, wherein the stretch mark removing cream comprises the crocus sativus extract. The crocus extract is crocus saponin crude product, crocus saponin finished product, crocus polysaccharide extract and/or crocus polysaccharide.
Preferably, the stretch mark removing cream comprises the following components in parts by weight:
phase A: 35-42 parts of water, 4-6 parts of glycerol, 0.01-0.03 part of EDTA-2Na and 0.10-0.30 part of allantoin;
phase B: 0.40-0.60 part of cetyl alcohol, 0.40-0.60 part of glyceryl stearate, 0.40-0.60 part of PEG-75 stearate, 0.78-0.60 part of ceteth-200.40, 1-3 parts of cetearyl alcohol, 0.40-0.60 part of cetearyl glucoside, 0.15-0.25 part of sodium stearyl glutamate, 4-8 parts of isononyl isononanoate, 0.15-0.25 part of tocopherol acetate and 1.5-2.5 parts of olive oil;
and C phase: 0.60-1.0 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.15-0.25 part of C18-21 alkyl and 0.32-0.25 part of tridecyl alcohol polyether-60.15;
phase E: 1.5-2.5 parts of butanediol, 0.40-0.60 part of jasminolone and 0.40-0.60 part of 1,2 hexanediol;
and (3) phase F: 13-17 parts of vital repair peptide, 4-6 parts of glycerol glucoside, 1.5-2.5 parts of isosorbide dimethyl ether, 8-12 parts of hydroxyprolinol CN, 3-7 parts of crocus sativus extract and 1.5-2.5 parts of asiaticoside;
phase H: 0.003 to 0.006 portion of essence.
Example 3
In the embodiment, the stretch mark removing cream comprises the following components in parts by weight:
phase A: 38.075 parts of water, 5.00 parts of glycerol, 0.02 part of EDTA-2Na and 0.20 part of allantoin;
phase B: 0.50 part of cetyl alcohol, 0.50 part of glycerol stearate, 0.50 part of PEG-75 stearate, 200.50 parts of ceteth-200.50 parts, 2.00 parts of cetearyl alcohol, 0.50 part of cetearyl glucoside, 0.20 part of sodium stearyl glutamate, 6.00 parts of isononyl isononanoate, 0.20 part of tocopherol acetate and 2.00 parts of olive oil;
and C phase: 0.80 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.20 part of C18-21 alkyl and 60.20 parts of tridecyl polyether;
phase E: 2.00 parts of butanediol, 0.50 part of jasminon and 0.50 part of 1,2 hexanediol;
and (3) phase F: 15.00 parts of ultimate repair peptide, 5.00 parts of glycerol glucoside, 2.00 parts of isosorbide dimethyl ether, 10.00 parts of hydroxyl prolinol CN, 5.00 parts of crocus sativus extract and 2.00 parts of asiaticoside;
phase H: 0.005 part of essence.
In the present invention, lease of a part of active ingredients is described as follows: the extreme repair peptide is a high-activity polypeptide component, and can repair and tighten skin in a layered manner in all directions; wherein hexapeptide-9 can effectively relieve and inhibit forehead raised lines, fine lines of the tail of the eye and the contraction and activity of peripheral muscles, help the muscles relax and recover the elastic tissues of the skin to be smooth lines; palmitoyl pentapeptide-4 belongs to an anti-aging component, and is used as a skin conditioner, an antioxidant and a humectant in cosmetics. Pentapeptides possess five amino acids, which is one of the most famous and prevalent collagen peptides in cosmetics. Palmitoyl pentapeptide-4 can stimulate the synthesis of key components of the skin matrix such as collagen and the like; palmitoyl tripeptide-1 is a matrikine signal peptide, which acts on the dermis layer, can promote the synthesis of extracellular matrix proteins (matrix proteins), particularly collagen, and simultaneously can increase the generation of elastin, hyaluronic acid, glycosaminoglycan and fibronectin, thereby strengthening the dermis layer, enabling the skin to become firmer, improving the moisture content of the skin, enabling the face skin to be plump and elastic, having stronger capability of resisting ultraviolet irradiation, and achieving the effect of beautifying the collagen injection. 2. The glyceryl glucoside has effects in activating cell activity and metabolism, activating regenerated skin cell, enhancing antioxidant activity (SOD) of skin cell, accelerating synthesis of type I collagen precursor in aging cell, increasing skin moisture retention, elasticity, smoothness and skin thickness (in vivo experiment), relieving skin redness, resisting erythra (in vivo experiment), accelerating wound healing and tissue repair. The dimethyl isosorbide anhydride promotes the functional raw materials to permeate the cortex to enable the effect to be stronger, the crocus sativus extract has the effects of deeply repairing sebum fibers, fading textures, promoting the synthesis of extracellular matrixes such as collagen and glycosaminoglycan, strengthening the dermis, enabling the skin to be thicker and firmer, and relieving wrinkles, and particularly has the outstanding effect on skin repair. Asiaticoside has effects of resisting depression and burn scar, resisting cancer, preventing and treating coronary atherosclerotic heart disease, myocardial infarction, cerebral thrombosis, cerebral infarction, improving memory, and whitening skin.
Example 4
Another aspect of the present invention is to provide a method for preparing the stretch mark removing cream, comprising the following steps: heating A, B phase to 80-90 deg.C, stirring, homogenizing, adding oil slowly into phase B and phase A, emulsifying, homogenizing for 2-5 min; adding C, D phase, homogenizing for 2-5 min, cooling, defoaming to 40-50 deg.C, adding E phase, dissolving, sequentially adding F, H phases, and homogenizing.
Example 5
In order to verify the technical effect of the invention, the stretch mark removing cream is prepared by the method of the embodiment 3 and the embodiment 4 according to the extract obtained in the embodiment 1 (specifically, the mass ratio of the crocin finished product to the crocin polysaccharide is 1: 1), and then the related performance test is carried out.
Selecting 100 striae gravidarum volunteers 25-35 years old, performing preliminary allergy test on the striae gravidarum of the volunteers, smearing the striae gravidarum on the volunteers at the same time every day, taking pictures to record the striae gravidarum condition of the volunteers, performing early-stage observation, and observing the striae gravidarum repairing condition and the fading degree in the time of one week, three weeks, four weeks, five weeks, six weeks and seven weeks respectively. The experimental data are summarized as follows:
table 1 summary of the results of the tests
As can be seen from the trademark, the product obtained by the invention has better striae gravidarum removing effect.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; as will be readily apparent to those skilled in the art from the disclosure herein, the present invention may be practiced without these specific details; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (9)
1. A method for extracting crocus sativus extract is characterized by comprising the following steps:
(1) pretreatment of raw materials: cleaning crocus sativus, removing dust and particulate impurities on the surface, cutting into pieces, and oven drying at 45-60 deg.C;
(2) wall breaking treatment of crocus sativus: pulverizing pretreated crocus sativus, adding 75-85% ethanol solution and crocus sativus according to the liquid-material weight ratio of 40-60:1, pulverizing, and breaking the wall in a wall breaking machine to obtain crocus sativus wall-broken liquid;
(3) extracting and processing crocin: ultrasonically extracting the wall-broken liquid of crocus sativus at 50-65 ℃ for 0.5-2 h, centrifuging to obtain crocin saponin extract and crocin residue, and spin-drying the crocin saponin extract with a rotary evaporator to obtain crocin saponin crude product;
(4) and (3) purifying crocin: diluting the crocin crude product with a proper amount of distilled water, adding the diluted crocin crude product into prepared adsorption resin for adsorption, eluting with distilled water, then eluting with 70-85% ethanol until no saponin flows out of the eluent, namely, completely eluting, collecting ethanol eluent, spin-drying with a rotary evaporator, and drying with a reduced pressure dryer to constant weight to obtain the crocin finished product:
(5) extracting and treating crocus sativus polysaccharide: adding distilled water 15-25 times the weight of the crocus sativus residue in the step (3), performing reflux extraction at 60-80 ℃ for 1-3 times after wall breaking treatment, extracting for 2-4.5h each time, and mixing water extract to obtain crocus sativus polysaccharide extract;
(6) decoloring the crocus polysaccharide extracting solution: adding a decolorizing agent 0.1-0.5 times of the weight of the crocus polysaccharide extract into the crocus polysaccharide extract, stirring for 20-40min at the rotating speed of 200-400r/min for decolorizing for 1-2 times, and filtering to obtain decolorized crocus polysaccharide extract;
(7) and (3) purifying crocus polysaccharide: concentrating the decolorized crocus sativus polysaccharide extract under reduced pressure, concentrating by 20-30 times, adding the rest extract into adsorption resin prepared in advance for adsorption, eluting with distilled water until no polysaccharide flows out of the eluate, collecting the eluate, and drying the eluate in a spray dryer to obtain crocus sativus polysaccharide.
2. The method for extracting crocus sativus extract according to claim 1, wherein: the extraction treatment of the crocin in the step (3) is continuous ultrasonic treatment, an ultrasonic extraction pipeline is arranged in the ultrasonic cavity, a continuous discharge pipe is arranged at one end of the ultrasonic extraction pipeline, and a double-spiral air inlet pipe for inputting hydraulic air and a liquid inlet pipe for inputting the wall breaking liquid of crocus are arranged at the other end of the ultrasonic extraction pipeline.
3. The method for extracting crocus sativus extract according to claim 2, wherein: the intake pipe with the two heliciform of feed liquor pipe stretch into to drawing the pipeline inner chamber, the inner chamber part that the intake pipe stretched into and draws the pipeline is equipped with the fumarole, the portion that the feed liquor pipe stretched into and draws the pipeline inner chamber is equipped with the spray tube.
4. The method for extracting crocus sativus extract according to claim 3, wherein: the aperture of the air injection hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole; the aperture of the liquid spraying hole is reduced in a gradient manner from the inner cavity of the extraction pipeline to the direction of the air inlet hole.
5. The method for extracting crocus sativus extract according to claim 4, wherein: the continuous discharging pipe is a spiral pipeline, and the tail end of the continuous discharging pipe is provided with an adjusting valve for flow control.
6. The stretch mark removing cream is characterized in that: comprising the crocus sativus extract of claim 1; the crocus extract is crocus saponin crude product, crocus saponin finished product, crocus polysaccharide extract and/or crocus polysaccharide.
7. The stretch mark removing cream according to claim 6, characterized by comprising the following components in parts by weight:
phase A: 35-42 parts of water, 4-6 parts of glycerol, 0.01-0.03 part of EDTA-2Na and 0.10-0.30 part of allantoin;
phase B: 0.40-0.60 part of cetyl alcohol, 0.40-0.60 part of glyceryl stearate, 0.40-0.60 part of PEG-75 stearate, 0.78-0.60 part of ceteth-200.40, 1-3 parts of cetearyl alcohol, 0.40-0.60 part of cetearyl glucoside, 0.15-0.25 part of sodium stearyl glutamate, 4-8 parts of isononyl isononanoate, 0.15-0.25 part of tocopherol acetate and 1.5-2.5 parts of olive oil;
and C phase: 0.60-1.0 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.15-0.25 part of C18-21 alkyl and 0.32-0.25 part of tridecyl alcohol polyether-60.15;
phase E: 1.5-2.5 parts of butanediol, 0.40-0.60 part of jasminolone and 0.40-0.60 part of 1,2 hexanediol;
and (3) phase F: 13-17 parts of vital repair peptide, 4-6 parts of glycerol glucoside, 1.5-2.5 parts of isosorbide dimethyl ether, 8-12 parts of hydroxyprolinol CN, 3-7 parts of crocus sativus extract and 1.5-2.5 parts of asiaticoside;
phase H: 0.003 to 0.006 portion of essence.
8. The stretch mark removing cream according to claim 6, characterized by comprising the following components in parts by weight:
phase A: 38.075 parts of water, 5.00 parts of glycerol, 0.02 part of EDTA-2Na and 0.20 part of allantoin;
phase B: 0.50 part of cetyl alcohol, 0.50 part of glycerol stearate, 0.50 part of PEG-75 stearate, 200.50 parts of ceteth-200.50 parts, 2.00 parts of cetearyl alcohol, 0.50 part of cetearyl glucoside, 0.20 part of sodium stearyl glutamate, 6.00 parts of isononyl isononanoate, 0.20 part of tocopherol acetate and 2.00 parts of olive oil;
and C phase: 0.80 part of hydrogenated lecithin;
phase D: 0.60 part of sodium polyacrylate, 0.20 part of C18-21 alkyl and 60.20 parts of tridecyl polyether;
phase E: 2.00 parts of butanediol, 0.50 part of jasminon and 0.50 part of 1,2 hexanediol;
and (3) phase F: 15.00 parts of ultimate repair peptide, 5.00 parts of glycerol glucoside, 2.00 parts of isosorbide dimethyl ether, 10.00 parts of hydroxyl prolinol CN, 5.00 parts of crocus sativus extract and 2.00 parts of asiaticoside;
phase H: 0.005 part of essence.
9. The preparation method of the stretch mark removing cream as claimed in any one of claims 6 to 8, which is characterized by comprising the following steps: heating A, B phase to 80-90 deg.C, stirring, homogenizing, adding oil slowly into phase B and phase A, emulsifying, homogenizing for 2-5 min; adding C, D phase, homogenizing for 2-5 min, cooling, defoaming to 40-50 deg.C, adding E phase, dissolving, sequentially adding F, H phases, and homogenizing.
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