CN112409456A - 干细胞细胞因子在制备化妆品或药物中的用途 - Google Patents
干细胞细胞因子在制备化妆品或药物中的用途 Download PDFInfo
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- CN112409456A CN112409456A CN202011316271.XA CN202011316271A CN112409456A CN 112409456 A CN112409456 A CN 112409456A CN 202011316271 A CN202011316271 A CN 202011316271A CN 112409456 A CN112409456 A CN 112409456A
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
表面分子 | 结果 |
CD29 | (99.33%±0.11%) |
CD73 | (99.74%±0.20%) |
CD90 | (99.96%±0.15%) |
CD34 | (2.01%±0.04%) |
类型 | VEGF(μg/L) | BDNF(μg/L) | HGF(μg/L) |
P3细胞不加多肽 | 988.2±36.9 | 123.4±13.5 | 1588.6±98.6 |
P3细胞加多肽 | 1645.1±55.7 | 197.4±28.7 | 2711.9±130.2 |
P10细胞不加多肽 | 1023.5±45.8 | 92.5±18.6 | 1236.5±84.2 |
P10细胞加多肽 | 1711.9±52.6 | 175.3±19.2 | 2281.2±90.1 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113384517A (zh) * | 2021-07-16 | 2021-09-14 | 天津汉青生物科技有限公司 | 干细胞细胞因子的制备方法及其在制备药物或化妆品中的用途 |
CN113476601A (zh) * | 2021-08-24 | 2021-10-08 | 天津汉青生物科技有限公司 | 干细胞细胞因子联合海藻多糖制备的药物或化妆品 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106701672A (zh) * | 2017-01-10 | 2017-05-24 | 广东康仹生命科技有限公司 | 一种人源脂肪间充质干细胞因子及其制备方法和用途 |
CN106924719A (zh) * | 2017-05-09 | 2017-07-07 | 广州润虹医药科技有限公司 | 含人干细胞因子的皮肤修复液及其制备方法 |
CN110025566A (zh) * | 2019-05-16 | 2019-07-19 | 三养健康科技有限公司 | 基于干细胞培养上清液的化妆品及其应用 |
-
2020
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106701672A (zh) * | 2017-01-10 | 2017-05-24 | 广东康仹生命科技有限公司 | 一种人源脂肪间充质干细胞因子及其制备方法和用途 |
CN106924719A (zh) * | 2017-05-09 | 2017-07-07 | 广州润虹医药科技有限公司 | 含人干细胞因子的皮肤修复液及其制备方法 |
CN110025566A (zh) * | 2019-05-16 | 2019-07-19 | 三养健康科技有限公司 | 基于干细胞培养上清液的化妆品及其应用 |
Non-Patent Citations (2)
Title |
---|
DAVOOD MEHRABANI ET AL.: "Adipose-Derived Stem Cells (ADSC) and Aesthetic Surgery:A Mini Review", 《WORLD J PLAST SURG》 * |
黄敏 等: "脂肪间充质干细胞条件培养液联合富血小板纤维蛋白修复小鼠皮肤损伤", 《中国组织工程研究》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113384517A (zh) * | 2021-07-16 | 2021-09-14 | 天津汉青生物科技有限公司 | 干细胞细胞因子的制备方法及其在制备药物或化妆品中的用途 |
CN113384517B (zh) * | 2021-07-16 | 2021-12-31 | 上海汉氏方舟生物科技有限公司 | 干细胞细胞因子的制备方法及其在制备药物或化妆品中的用途 |
CN113476601A (zh) * | 2021-08-24 | 2021-10-08 | 天津汉青生物科技有限公司 | 干细胞细胞因子联合海藻多糖制备的药物或化妆品 |
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