CN112369315A - Breeding method of hydrangea - Google Patents
Breeding method of hydrangea Download PDFInfo
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- CN112369315A CN112369315A CN202011289097.4A CN202011289097A CN112369315A CN 112369315 A CN112369315 A CN 112369315A CN 202011289097 A CN202011289097 A CN 202011289097A CN 112369315 A CN112369315 A CN 112369315A
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- 235000014486 Hydrangea macrophylla Nutrition 0.000 title claims abstract description 67
- 241001092080 Hydrangea Species 0.000 title claims abstract description 66
- 238000009395 breeding Methods 0.000 title claims abstract description 33
- 238000005520 cutting process Methods 0.000 claims abstract description 137
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 74
- 230000001488 breeding effect Effects 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 26
- 238000012258 culturing Methods 0.000 claims abstract description 19
- 230000003134 recirculating effect Effects 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 description 38
- 239000005556 hormone Substances 0.000 description 14
- 229940088597 hormone Drugs 0.000 description 14
- 238000011160 research Methods 0.000 description 14
- 230000012010 growth Effects 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
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- 230000008569 process Effects 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000003501 hydroponics Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000006479 redox reaction Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000005068 transpiration Effects 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000267823 Hydrangea macrophylla Species 0.000 description 1
- 241000511974 Hydrangea paniculata Species 0.000 description 1
- 241001091442 Hydrangeaceae Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 238000000338 in vitro Methods 0.000 description 1
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- 208000021267 infertility disease Diseases 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a method for breeding hydrangea, which comprises the following steps: culturing the hydrangea cutting with at least two sections in water environment to obtain the water culture seedling with adventitious roots, wherein the sections at the lower end of the cutting are inserted into water. Compared with the hydrangea cuttage and tissue culture breeding mode, the provided hydrangea breeding method is simple to operate, does not need strict breeding conditions, is low in cost and pollution-free, and is high in hydrangea rooting rate and large in rooting number.
Description
Technical Field
The invention relates to the technical field of plant breeding, in particular to a method for breeding hydrangea.
Background
Hydrangea (Hydrangea macrophylla) is a small shrub of Hydrangea genus of hydrangeaceae family, and is called Hydrangea, and Ziyang flower, etc., and is suitable for planting in warm, humid and semi-yin environment at 18-28 deg.C. Because the method can be widely managed, the plant diseases and insect pests are less, the inflorescence is large, the method is widely applied to urban beautification, garden landscaping, pot culture, cut flower and other aspects, the hydrangea industry is rapidly developed in China, and the hydrangea also becomes one of flower varieties popular with people.
At present, the hydrangea varieties planted in China are introduced from foreign countries. The most ornamental part of hydrangea is a large inflorescence composed of many infertile flowers, so the more ornamental varieties are, the more difficult the seeds are to obtain. Meanwhile, the shape of hydrangea seeds is extremely small, the collection and sowing are very difficult, and the mass production cannot be realized. In view of this, the improvement of the hydrangea propagation technology is particularly important for the development of the hydrangea industry.
The existing hydrangea breeding mainly adopts methods of cuttage and tissue culture breeding. In the fields of Effects of growth regulator and substrate on culture greening of organic hydrange, the research of cuttage breeding technology is carried out by using semi-lignified twigs of hydrangea paniculata lime lamps as materials, and the fact that the rooting rate of the vermiculite and peat mixed matrix cuttage is the highest and reaches 84% in seven matrix combinations is found, and the rooting promoting effect of ABT and NAA is the best. Zhang Guoshua in Research on culture propagation of hydrangea uses vermiculite as matrix, and researches show that the rooting effect is best when the upper part of the branch is used as cutting. Wuhuafen in Tissue culture and rapid propagation of Hydrangea by MS +6-BA 1.0 mg.L-1+IBA 0.05mg·L-1As a substrate, the differentiation and proliferation effects of the hydrangea are obvious, and the rooting effect is better when the in vitro rooting is carried out in the environment with the humidity of 85 percent. However, the breeding method of hydrangea has higher requirements on experimental conditions. The cuttage breeding requires a specific substrate, a high hormone concentration and a specific culture environment, the cost is high, the operation is relatively complex, and the environment pollution is caused by using the hormone with an excessively high concentration in the test process. The tissue culture breeding is more complex in related procedures, experimental operation needs to be completed under laboratory conditions, the culture process needs to be completed under aseptic conditions, different culture media are needed in different culture stages, and the method has no general popularization value.
In view of the above, a new technical scheme for breeding hydrangea needs to be provided to solve the above technical problems.
Disclosure of Invention
The invention aims to provide a method for breeding hydrangea with low cost, no pollution, high rooting rate and large number of roots.
In order to achieve the purpose, the invention adopts the following technical means:
a method for breeding hydrangea comprises the following steps: culturing the hydrangea cutting with at least two sections in water environment to obtain hydrangea hydroponic seedlings with adventitious roots; wherein the section at the lower end of the cutting is inserted into the water.
Preferably, the upper end cut of the cutting is a flat cut, and the lower end cut of the cutting is a bevel cut.
Preferably, the section at the upper end of the cutting strip keeps 2-4 blades, and the area of each blade is 1/3-2/3 of the area of the original blade.
Preferably, the distance between the upper end notch and the section at the upper end of the cutting slip is 1-3 cm.
Preferably, the distance between the lower end notch and the section of the lower end of the cutting slip is 1-3 cm.
Preferably, the angle of the beveling is 40 ° to 50 °.
Preferably, the length of the cutting is 5-10 cm.
Preferably, the concentration of NAA in the water environment is 0-5 mg/L.
Preferably, the concentration of IBA in the aqueous environment is 0-5 mg/L.
Preferably, the aqueous environment comprises a recirculating aqueous environment.
Preferably, the cycle period of the circularly flowing water environment is 20-28 h.
Preferably, the culturing time is 25 to 40 days.
Preferably, the light culture condition of the culture is 12-16 h/day, and the culture temperature is 24-28 ℃; the dark culture condition is 8-12 h/day, and the culture temperature is 18-22 ℃.
Compared with the prior art, the invention has the following technical effects:
compared with the cutting and tissue culture breeding modes of the hydrangea, the breeding method of the hydrangea with the water culture seedlings with adventitious roots by culturing the hydrangea cuttings reserved with at least two nodes has the advantages of simple operation, no need of strict breeding conditions, low cost, no pollution, high rooting rate, more roots and the like.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 shows the rooting rate of hydrangea cuttings cultured under hydroponic conditions of example 1, example 2 and example 3 as a function of hydroponic time;
FIG. 2 shows the relationship between hormone concentration and rooting rate of hydrangea cuttings in hydroponic conditions of example 1, example 2 and example 3, and reference examples 1 to 8;
FIG. 3 shows the relationship between hormone concentration and the number of roots of hydrangea cutting under hydroponic conditions of example 1, example 2 and example 3, and reference examples 1 to 8;
FIG. 4 shows the relationship between hormone concentration and average root length of hydrangea cuttings in hydroponic conditions of example 1, example 2 and example 3, and reference examples 1 to 8;
FIG. 5 shows the color of hydrangea cuttings cultured under hydroponic conditions of example 1, example 2, reference example 1, and reference example 2.
Detailed Description
The invention provides a method for breeding hydrangea, which comprises the following steps: culturing the hydrangea cutting with at least two sections in water environment to obtain hydrangea hydroponic seedlings with adventitious roots, wherein the sections at the lower end of the cutting are inserted into water. As can be understood by those skilled in the art, the upper end node of the hydrangea cutting can grow branches, and more meristems are arranged around the lower end node, so that adventitious roots are more likely to grow. Therefore, the lower nodes of the cuttings are inserted into water and cultured, so that adventitious roots can grow around the lower nodes. Therefore, the hydrangea hydroponics breeding process provided by the invention is simple to operate, only the hydrangea cutting comprising at least two sections needs to be prepared, and the cost is low. Meanwhile, the method has low requirements on the environment and conditions of breeding, the cuttings are only required to be placed in the water environment for culture, and the water culture seedling can be carried out in the places with good illumination conditions such as windowsills, balconies and the like in seasons with proper temperature conditions under the condition of meeting the water culture conditions.
In certain embodiments of the invention, the cutting is made by cutting the current year hydrangea branch from the top down. Of course, the invention can be practiced with other hydrangea branches. When the branch is cut from the top end to the lower end, the top bud is reserved on the node at the upper end of the upper section cutting. As can be understood by those skilled in the art, the cuttings at the upper section are cuttings with terminal buds, are tender, have low lignification degree and developed meristem, and thus adventitious roots are easy to grow around the lower end nodes of the cuttings. When the branches are cut into sections from the top end to the bottom, top buds are not reserved on the sections of the upper ends of the obtained middle-section and lower-section cuttings, the cuts of the upper ends of the cuttings are cut flatly, and the cuts of the lower ends of the cuttings are cut obliquely. The incision on the upper end of the cutting can be cut flatly to reduce the wound area of the cutting and reduce the evaporation of water. The beveling of the cut at the lower end of the cutting is to increase the wound area of the cutting, so that the cutting can absorb moisture and callus can be generated, and the rooting of the cutting can be promoted.
In some embodiments of the invention, the section of the upper end of the slip comprises 2-3 blades, and the area of the blades is 1/3-2/3 of the area of the original blades. As can be appreciated by those skilled in the art, the cuttings require nutrients during rooting, and therefore, the leaves are retained to facilitate photosynthesis of the cuttings, thereby providing nutrients for rooting of the cuttings. However, the leaves are retained too much, and the transpiration of the cuttings is strengthened, so that the cuttings are easy to wither. Therefore, the area of the leaf is kept about half of the area of the original leaf, and the balance between photosynthesis and transpiration of the cutting can be maintained.
In some embodiments of the invention, the cut at the upper end of the slip is 1-3cm from the section at the upper end of the slip. As can be understood by those skilled in the art, the upper node is provided with leaves, and the 1-3cm part of the meristem around the upper node is relatively more easy to grow out branches.
In some embodiments of the invention, the cut at the lower end of the slip is 1-3cm from the joint of the lower end of the slip. As will be appreciated by those skilled in the art, meristems at 1-3cm around the lower node are relatively numerous and more prone to adventitious root formation.
In certain embodiments of the invention, the angle of the chamfer is 40 ° to 50 °. As can be understood by those skilled in the art, the cut area is largest when the angle of the beveling is 40-50 degrees, so that the cuttings can absorb water favorably, and the callus can be generated favorably, so that the rooting of the cuttings is promoted.
In certain embodiments of the invention, the length of the cutting is 5-10 cm. As can be understood by those skilled in the art, too short a cutting can result in too few cuttings to be inserted into the water, affecting the amount of rooting; too long cuttings can increase the water transportation length, possibly cause the cuttings to lose water and wither, and further reduce the survival rate of the cuttings.
In certain embodiments of the invention, the concentration of NAA in the aqueous environment is 0-5 mg/L. In the water environment, when the concentration of NAA is less than 5mg/L or even no NAA is added, the rooting rate and the rooting number of the cuttings are the highest.
In certain embodiments of the invention, the concentration of IBA in the aqueous environment is 0-5 mg/L. In the water environment, when the concentration of IBA is less than 5mg/L or even no IBA is added, the rooting rate and the rooting number of the cuttings are the highest.
In certain embodiments of the invention, the aqueous environment comprises a recirculating aqueous environment. It will be appreciated by those skilled in the art that a recirculating flowing aqueous environment can maintain a steady water level. Meanwhile, the water environment of the circulating flow can also increase oxygen in water, which is more beneficial to the growth of roots.
In certain embodiments of the invention, the circulating aqueous environment has a cycle period of 20 to 28 hours. It will be appreciated by those skilled in the art that the water used for hydroponics may be circulated approximately once a day, with the specific number and period of cycles depending on the water level of the water environment to keep the lower portion of the cuttings in the water at all times.
In certain embodiments of the invention, the culturing is for a period of 25 to 40 days. As will be appreciated by those skilled in the art, the time for the hydrangea cuttings to begin rooting is about 15 days. The average root length of the water culture seedlings can reach 3-5cm about 10-25 days after the cuttings take root, and the transplanting requirements are met.
In some embodiments of the invention, the light culture conditions of the culture are 12-16 h/day, and the culture temperature is 24-28 ℃; the dark culture condition is 8-12 h/day, and the culture temperature is 18-22 ℃. As can be understood by those skilled in the art, more robust water-cultured seedlings can be obtained under the condition that the illumination time is 12-16 hours.
The invention is further described below with reference to specific embodiments and the attached drawings.
In a specific embodiment of the invention, the hydrangea explant hydroponic breeding test is performed in a light incubator (Shanghai Bochner BSG-800).
The hydrangea adopted in the embodiment of the invention is a big hydrangea branch of the same year, which is introduced from the university of georgia in the United states and has consistent growth vigor.
In the specific embodiment of the invention, the number of rooted explants is counted every 3 days from 15 d. And after culturing for 36d, counting the final explant rooting number, the rooting number and the root length of each explant, and carrying out statistical analysis on test data by using Excel.
Example 1
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end section is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section of the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades of the section of the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, and setting the light-dark culture condition at 16h/8h, the temperature under the light culture condition at 25 ℃ and the temperature under the dark culture condition at 20 ℃.
Example 2
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end section is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section of the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades of the section of the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of NAA in the sterile water is 5mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 1
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end section is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section of the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades of the section of the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of NAA in the sterile water is 10mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 2
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end section is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section of the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades of the section of the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of NAA in the sterile water is 15mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 3
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of NAA in the sterile water is 20mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 4
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of NAA in the sterile water is 25mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Example 3
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of IBA in the sterile water is 5mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 5
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of IBA in the sterile water is 10mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 6
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of IBA in the sterile water is 15mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 7
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of IBA in the sterile water is 20mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
Reference example 8
And selecting the current-year branches with consistent growth vigor as research objects. Each branch is cut into three sections of cuttings, namely an upper section, a middle section and a lower section, two sections are reserved in each section of cuttings, and the length of each section is 10 cm. When the cutting is trimmed, the cut is smooth, the distance between the cut at the upper end and the section at the upper end is 2cm, and the cut is a flat opening; the distance between the lower end notch and the lower end is 2cm, the notch is an oblique notch, and the notch angle is 45 degrees. The section at the upper end of the cutting remains 2 blades, the size of the blade remains 1/2, and the blades at the section at the lower end are all removed.
Culturing 30 cuttings in sterile water, changing the sterile water every 24 hours, wherein the concentration of IBA in the sterile water is 25mg/L, the light-dark culture condition is 16h/8h, the temperature under the light-dark culture condition is set to be 25 ℃, and the temperature under the dark culture condition is set to be 20 ℃.
The rooting time, rooting rate, rooting number, root length and skin color of the hydrangea cuttings in the examples and the reference examples are as follows:
as can be seen from the above table, the hydrangea cutting has the highest rooting rate under the water culture condition of sterile water, 5mg/L of sterile water of NAA content and 5mg/L of sterile water of IBA content, respectively, 96.2%, 96.1% and 84.2%. The rooting rate of the hydrangea cutting is highest under the condition of no hormone treatment. The average root number of each plant under the sterile water condition is 30.45, while the root number under the NAA and IBA treatment conditions is 21.4 and 18.78 respectively, which is obviously reduced compared with the root number under the sterile water condition. The average root length per plant under the sterile water and NAA treatment conditions was not significantly different, but the average root length per plant under the IBA treatment conditions was the shortest, only 32.15 mm.
As can be seen from the table above, different hormone concentrations have different effects on the rooting of the cuttings, the low concentration can promote the rooting of the cuttings, and the high concentration has an inhibiting effect on the roots. The results of treating cuttings with NAA of different concentrations show that the average rooting rate reaches the peak value of 96.2% when the concentration is 0 mg/L; 96.1% under the condition of 5mg/L concentration treatment; when the concentration is higher than 10mg/L, the average rooting rate tends to be low. The average root number per plant is the largest when the concentration is 0mg/L, and is 30.45, the concentration is increased, and the average root number per plant is reduced. The average root length per plant is in a descending trend along with the increase of the concentration of the NAA, the maximum is 45.46 at 0mg/L, the concentration is slightly reduced at 5mg/L and 10mg/L, and the average root length per plant of the explant is obviously reduced when the concentration is higher than 15 mg/L. From the above table, it can be seen that the surface of the cuttings immersed in the solution turns red significantly when the NAA concentration is 15mg/L or more. This is because, too high hormone concentration induces the cutting to generate secondary metabolites, which affect some redox reactions inside the cutting, hamper the normal physiological metabolism of root growth, thus inhibiting rooting and even producing toxic effects. The table shows that the induction effect of 0mg/L concentration treatment on cutting rooting is the best, the NAA (5mg/L and 10mg/L) treatment with lower concentration has a certain promotion effect on the cutting rooting of hydrangea, and the cutting rooting is obviously inhibited by high concentration hormone treatment.
The inventor discovers that during the hydroponic rooting process of hydrangea, various hormones with proper concentrations are selected as a key factor for success or failure of hydrangea hydroponics. The exogenous hormone can regulate the content of related endogenous hormones in the explant, and induce cell differentiation, so that the formation of adventitious roots is promoted. The hormone with lower concentration can promote the formation of callus, and is favorable for the cell to be differentiated into root primordium, thereby promoting rooting. The hormone with too high concentration can induce the cutting to generate secondary metabolite, which affects some oxidation-reduction reaction inside the cutting, and hinders the normal physiological metabolism of root growth, thereby inhibiting rooting and even generating toxic action. Meanwhile, the osmotic pressure of the solution is increased due to too high concentration of the phytohormone, so that the cuttings cannot utilize the water in the solution, the rooting rate is reduced, and in a more serious case, the cuttings are dehydrated and die.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (10)
1. A method for breeding hydrangea, which is characterized in that,
the method comprises the following steps:
culturing the hydrangea cutting with at least two sections in water environment to obtain hydrangea hydroponic seedlings with adventitious roots; wherein the section at the lower end of the cutting is inserted into the water.
2. The method for breeding hydrangea as claimed in claim 1, wherein:
the upper end cut of the cutting is a flat cut, and the lower end cut of the cutting is a bevel cut.
3. The method for breeding hydrangea as claimed in claim 1, wherein:
2-4 blades are reserved at the upper end of the cutting, and the area of each blade is 1/3-2/3 of the area of the original blade.
4. The method for breeding hydrangea as claimed in claim 2, wherein:
the distance between the upper end notch and the section at the upper end of the cutting is 1-3 cm;
the distance between the lower end notch and the section of the lower end of the cutting is 1-3 cm;
the angle of the beveling is 40 ° to 50 °.
5. The method for breeding hydrangea as claimed in claim 1, wherein:
the length of the cutting is 5-10 cm.
6. The method for breeding hydrangea as claimed in claim 1, wherein:
the concentration of NAA in the water environment is 0-5 mg/L; or
The concentration of IBA in the water environment is 0-5 mg/L.
7. The method for breeding hydrangea as claimed in claim 1, wherein:
the aqueous environment includes a recirculating aqueous environment.
8. The method for breeding hydrangea as claimed in claim 7, wherein:
the cycle period of the circularly flowing water environment is 20-28 h.
9. The method for breeding hydrangea as claimed in claim 1, wherein:
the culture time is 25-40 days.
10. The method for breeding hydrangea as claimed in claim 1, wherein:
the light culture condition of the culture is 12-16 h/day, and the culture temperature is 24-28 ℃; the dark culture condition is 8-12 h/day, and the culture temperature is 18-22 ℃.
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