CN112293741A - Plant extract and application thereof - Google Patents

Plant extract and application thereof Download PDF

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Publication number
CN112293741A
CN112293741A CN202011198352.4A CN202011198352A CN112293741A CN 112293741 A CN112293741 A CN 112293741A CN 202011198352 A CN202011198352 A CN 202011198352A CN 112293741 A CN112293741 A CN 112293741A
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plant extract
groups
group
feed
added
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虞德兵
沈雪峰
李晶
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Jiangsu Hongkang Biotechnology Co ltd
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Jiangsu Hongkang Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a plant extract and application thereof, wherein the plant extract comprises the following components in percentage by mass: the feed is applied to chicken feeds, wherein 32% of houttuynia cordata, 16% of dandelion, 6% of honeysuckle, 15% of astragalus mongholicus, 11% of turmeric and 20% of purslane are added into the basic chicken feed in a manner of adding 200-1200 mg of plant extract per kg of the basic chicken feed. The additive prepared by the invention is added into poultry feed, can effectively improve the quality of eggs, and replaces a large amount of hormones for use.

Description

Plant extract and application thereof
Technical Field
The invention belongs to the technical field of poultry and animal feeding, and particularly relates to a plant extract and application thereof.
Background
With the increasing maturity of poultry animal feeding technology, specialized poultry animal feed is also the figure eight. Compared with the traditional poultry animal food, the animal feed is more sanitary and healthy and has balanced nutrition. In order to meet certain specific requirements, different animal feed additives are added into the feed of the poultry, and the feed additives refer to small or trace substances added in the processes of production, processing and use of the feed, and have small dosage but remarkable effect in the feed. The feed additive is a raw material inevitably used in modern feed industry, and has obvious effects on strengthening the nutritive value of basic feed, improving the production performance of poultry, ensuring the health of poultry, saving the feed cost, improving the quality of poultry and egg products and the like. The poultry animal feed on the market can meet the nutritional requirements of poultry raising, but the epidemic prevention capability of the poultry is enhanced, and the trees are not built much in the aspect of inhibiting the propagation and growth of harmful bacteria. In order to prevent and treat animal diseases, a large amount of hormones are added to feed, which results in the degradation of the quality of eggs or meat.
In the technical research and development field of the industry, an additive which does not contain a large amount of hormone and is prepared by taking herbs as a main body for enhancing the immunity of animals is urgently needed.
Object of the Invention
Aiming at the defects of the prior art, the invention improves the quality of poultry eggs and meat, and summarizes a plant extract and application thereof by mixing and combining herbal powder in order to prevent and treat the current situation that a large amount of hormones are used for epidemic diseases, so that the immunity of poultry can be effectively improved, and the quality of poultry eggs is improved.
Disclosure of Invention
In order to achieve the above object, the plant extract of the present invention comprises, by mass: the traditional Chinese medicine composition comprises the following components, by weight, 32% of houttuynia cordata, 16% of dandelion, 6% of honeysuckle, 15% of astragalus membranaceus, 11% of turmeric and 20% of purslane, wherein the houttuynia cordata, the dandelion, the honeysuckle, the astragalus membranaceus, the turmeric and the purslane are all dry plant powder, and the plant extract is a mixture of various dry plant powder.
The application of the plant extract in chicken feed is characterized in that the plant extract is added into the basic chicken feed in a manner of adding 200-1200 mg per kg.
Further, the present invention provides a preferable mode in which the plant extract is added to the basal chicken feed in an amount of 400mg per kg.
Still further, the present invention provides another preferable mode, wherein the plant extract is added to the basal chicken feed in a manner of adding 800mg per kg.
The dried plant powder is a powder obtained by processing houttuynia cordata, dandelion, honeysuckle, astragalus mongholicus, turmeric and purslane through a wall-breaking crushing process, the wall-breaking rate is over 90%, and the particle size is larger than 180 meshes.
Has the advantages that: compared with the prior art, the invention has the beneficial effects that:
1. the additive prepared by the invention is added into poultry feed, can effectively improve the quality of egg products, and replaces a large amount of hormones.
2. The additive prepared by the invention is added into poultry feed, so that the quality of chicken can be effectively improved, and the immunity of poultry is enhanced.
3. The additive can be used in the whole period of poultry production, has no medicine residue and no drug resistance, and is safe and reliable.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and the embodiments described below are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
A plant extract comprising, in mass percent: the traditional Chinese medicine composition comprises the following components, by weight, 32% of houttuynia cordata, 16% of dandelion, 6% of honeysuckle, 15% of astragalus membranaceus, 11% of turmeric and 20% of purslane, wherein the houttuynia cordata, the dandelion, the honeysuckle, the astragalus membranaceus, the turmeric and the purslane are all dry plant powder, and the plant extract is a mixture of various dry plant powder.
The plant extract is applied to chicken feed, and 200-1200 mg of the plant extract is added into basic chicken feed per kg of the plant extract.
The plant extract was added to the basal chicken feed in an amount of 400mg per kg.
Plant extract is added to the basal chicken feed in a manner of adding 800mg per kg.
1. Proof of experiment
1.1 test animals
Selecting a dwarf laying hen strain.
1.2 Experimental design and Breeding management
495 healthy laying hens with similar weights were selected and randomly divided into 5 groups of 3 replicates each of 33 chickens (the feed consumption test was 9 replicates each of 11 chickens each). The control group was fed with a basal diet (1 group), and the 4 treatment groups were fed with 200, 400, 800 and 1200mg/kg of plant extracts (2, 3, 4 and 5 groups, respectively) added to the basal diet, and the test was started at 270 days of age in the post-egg laying period for 8 weeks (3 months, 4 days to 4 months, 29 days). During the whole experiment, three-layer stepwise individuals were raised in cages, freely drunk water, irradiated by conventional light (16h:8h) and immunized.
Group 1 2 groups of Group 3 4 groups of 5 groups of
Addition amount (mg/kg) 0 200 400 800 1200
Body weight (g) before test 1504.3±191.0 1518.0±189.4 1522.9±213.6 1475.4±195.9 1495.3±198.3
Egg production (%) 84.2 84.2 83.4 84.8 84.7
1.3 sample Collection and measurement
During the test period, the egg number, breakage, death and elutriation and other conditions of each group are recorded every day; measuring the weight of eggs in the whole group every week; measuring indexes (such as sand shell and dark spot) of whole group of eggs every 2 weeks, and measuring conventional egg quality (more than 30 eggs/group) including color, glossiness, strength, weight, height, Haugh unit, thickness, etc.; at the end of 4 weeks and 8 weeks, randomly selecting 2 in each group, collecting blood to detect serum oxidative stress indexes (total antioxidant capacity (T-AOC), superoxide dismutase (SOD), Malondialdehyde (MDA), Catalase (CAT), glutathione peroxide (GSH-PX), reduced Glutathione (GSH) and oxidized glutathione (GSSG) content), immunological indexes (serum immunoglobulin IgG, IgM, IgA, complement 3(C3) and complement 4(C4) content, phagocytic capacity of phagocytic cells in 8 weeks), performing conventional slaughter, namely weighing before slaughter, weighing whole chest, weighing semi chest, abdominal fat, etc., taking thymus, spleen, bursa to detect immune organ index, and taking liver to detect liver tissue oxidative stress index.
Blood collection: repeating the 2 times 3 times 5 groups, collecting 3 ml of blood from each chicken, subpackaging the blood into 2 tubes of 1.5 ml centrifuge tubes, centrifuging, taking supernatant, namely serum, and storing at-20 ℃. (index for measuring oxidative stress in serum, immunological index)
Sampling: 2X 3 repeated X5 groups, after slaughtering, part of liver tissue is cut into small pieces and put into a 1.5 ml centrifuge tube (for measuring oxidative stress index). Loading the contents of jejunum, ileum and cecum into 5 ml centrifuge tube, loading another part of liver tissue into 1.5 ml centrifuge tube (to be tested for TOR sample receptor signal channel related gene expression), and storing at-80 deg.C.
Slaughtering: 5X 3 repeated X5 groups, and performing conventional slaughtering to determine slaughtering rate, half-bore rate, full-bore rate, pectoral muscle rate, leg muscle rate, liver rate, abdominal fat rate, and fatty liver degree. Weighing thymus, spleen and bursa of Fabricius, and measuring immune organ index.
Phagocytic capacity of phagocytic cells: repeating 2 × 3 × 5 groups, weighing, injecting 0.2ml India ink diluted 1: 3 into intra-winged vein, timing, collecting 0.2ml blood from heart 2min and 10min after injecting ink, and adding 20ml 0.1% Na2CO3Shaking the solution evenly, measuring the light absorption value OD at the wavelength of 600nm by using an enzyme-linked immunosorbent assay, and taking Na as the reference2CO3The solution was used as a blank control, the chicken was slaughtered, the liver and spleen were weighed, and the phagocytic index was calculated.
Phagocytosis index: a ═ body weight ÷ (liver weight + spleen weight) × K1/3. Description of the drawings: a is the phagocytic index; k is carbon clearance rate, and K is (lgOD1-lgOD2)/t2-t 1; OD1 and OD2 are absorbance values of the solution to be detected prepared by collecting blood for 2min and 10min respectively; t1 and t2 indicate blood collection time after indian ink injection.
2 results and analysis
2.1 egg quality
2.1.12 weeks of egg quality
The results are shown in table 1, with the gloss 2, 3, 5 groups being significantly greater than 1 group; the eggshell thickness 4, 5 groups are significantly larger than 1, 2, 3 groups; egg yolk color 2 group was significantly greater than 1 group.
Figure BDA0002754637920000041
Egg quality at Table 12 weeks
2.1.24 weeks of egg quality
The results are shown in table 2, with 5 groups of eggshell color significantly greater than 4 groups, 5 groups of eggshell thickness significantly less than the other groups, 1 group of eggshell weight significantly less than 2, 3, 4 groups, and 1 group of yolk color significantly less than 2 groups.
Figure BDA0002754637920000042
Egg quality at 24 weeks in Table
2.1.36 weeks of egg quality
The results are shown in table 3, with gloss 2, 3, 4, 5 groups being significantly greater than 1 group, and 5 groups being significantly greater than the other groups.
Figure BDA0002754637920000043
Egg quality of table 36 weeks
2.1.48 Weeked egg quality
The results are shown in table 4, with eggshell color 3 significantly greater than 1; gloss 4, 5 groups were significantly less than 1, 2, 3 groups; the eggshell thicknesses of 3, 4 and 5 groups are obviously less than 1 group, and the eggshell thicknesses of 5 groups are obviously less than 1 and 2 groups; yolk ratio 4, 5 groups were significantly greater than 1, 2 groups; the hamlet unit 4 groups were significantly smaller than groups 1, 2, 3.
Figure BDA0002754637920000051
Egg quality at 48 weeks in Table
2.2 oxidative stress index
2.2.1 index of oxidative stress in the liver
The results are shown in Table 5, and the T-AOC and GSH-PX groups have no significant difference from the 1 group.
Figure BDA0002754637920000052
TABLE 58 week hepatic oxidative stress index
2.2.24 week serum oxidative stress index
The results are shown in Table 6, where T-AOC 3, 4, 5 groups are significantly greater than 1 group; SOD 3, 4, 5 groups are significantly larger than 1 group; the CAT 5 group was significantly larger than the 1, 2 groups; GSH-PX4, group 5 was significantly greater than groups 1, 2; the GSH 3 group was significantly larger than the 1 group.
Figure BDA0002754637920000053
TABLE 64 week serum oxidative stress index
2.2.38 week serum oxidative stress index
The results are shown in Table 7, where T-AOC 3, 4, 5 groups are significantly greater than 1, 2 groups; SOD 3, 4, 5 groups are significantly larger than 1 group; the MDA 5 group is significantly smaller than the 1 and 2 groups; CAT 4, 5 groups were significantly greater than 1 group; the GSH-PX4, 5 group was significantly greater than group 1.
Figure BDA0002754637920000061
TABLE 78 serum oxidative stress indicators for weeks
2.5 immunological indices
2.5.14 immunological indicators of the week serum
The results are shown in table 8, IgA 5 group is significantly greater than group 1; the IgG 2 and 3 groups are obviously larger than the 1 group, and the 4 and 5 groups are obviously larger than the 1 and 2 groups; groups IgM 3, 4, 5 were significantly larger than groups 1, group 5 was significantly larger than groups 1, 2; both C3 and C4 were groups 3, 4, 5 significantly larger than group 1.
Figure BDA0002754637920000062
TABLE 84 week serum immunological indices
2.5.28 immunological indicators of the week serum
The results are shown in table 9, IgG 3, 4, 5 groups are significantly larger than 1, 2 groups; groups IgM 3, 4, 5 were significantly larger than groups 1, group 5 was significantly larger than groups 1, 2; group C34, 5 is significantly larger than group 1, 2; groups C43, 4, 5 were significantly larger than group 1.
Figure BDA0002754637920000063
TABLE 98 week serum immunological indices
The plant extract and the application thereof provided by the embodiment of the invention are described in detail above, the principle and the embodiment of the invention are explained by applying specific embodiments, and the description of the embodiment is only used for the main synthesis thought and route of the invention; in addition, in view of the above, the present disclosure should not be construed as limited to the present disclosure, but is to be construed as an exemplification and illustration of the present disclosure.

Claims (4)

1. A plant extract characterized by: the plant extract comprises the following components in percentage by mass: the traditional Chinese medicine composition comprises the following components, by weight, 32% of houttuynia cordata, 16% of dandelion, 6% of honeysuckle, 15% of astragalus membranaceus, 11% of turmeric and 20% of purslane, wherein the houttuynia cordata, the dandelion, the honeysuckle, the astragalus membranaceus, the turmeric and the purslane are all dry plant powder, and the plant extract is a mixture of various dry plant powder.
2. The application of the plant extract is characterized in that: the plant extract is applied to chicken feed, and 200-1200 mg of the plant extract is added into basic chicken feed per kg of the plant extract.
3. Use of a plant extract according to claim 2, wherein: the plant extract was added to the basal chicken feed in an amount of 400mg per kg.
4. Use of a plant extract according to claim 2, wherein: plant extract is added to the basal chicken feed in a manner of adding 800mg per kg.
CN202011198352.4A 2020-10-31 2020-10-31 Plant extract and application thereof Pending CN112293741A (en)

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040161524A1 (en) * 2001-06-21 2004-08-19 Yasushi Sakai Process for producing a plant extract containing plant powder
CN101926411A (en) * 2010-09-14 2010-12-29 丽水市林业科学研究院 Heartleaf houttuynia herb livestock and poultry feed additive and preparation method thereof
CN102771625A (en) * 2012-08-08 2012-11-14 陈虹 Chinese herbal medicine feed supplement and usage method for improving egg quality
CN103947875A (en) * 2014-04-15 2014-07-30 河南师范大学 Chinese medicinal feed additive and application thereof
CN104082530A (en) * 2014-06-13 2014-10-08 思南县常青种养专业合作社 Chinese herbal medicine additive for chicken feed
CN104187170A (en) * 2014-09-12 2014-12-10 安徽农业大学 Green tea composite chicken feed additive capable of enhancing immunity of chickens
CN104621408A (en) * 2015-02-12 2015-05-20 贵州省畜牧兽医研究所 Functional feed for improving immunity of laying hens and preparation method of functional feed
CN104839516A (en) * 2015-06-24 2015-08-19 福建亚森农业综合开发股份公司 Compound poultry feed
CN108310136A (en) * 2018-04-20 2018-07-24 佛山迁宇科技有限公司 A kind of feed addictive and preparation method thereof of prevention white diarrhea
CN109588572A (en) * 2019-01-30 2019-04-09 永胜腾飞家禽养殖有限公司 A kind of feed addictive of layer chicken improving egg quality

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040161524A1 (en) * 2001-06-21 2004-08-19 Yasushi Sakai Process for producing a plant extract containing plant powder
CN101926411A (en) * 2010-09-14 2010-12-29 丽水市林业科学研究院 Heartleaf houttuynia herb livestock and poultry feed additive and preparation method thereof
CN102771625A (en) * 2012-08-08 2012-11-14 陈虹 Chinese herbal medicine feed supplement and usage method for improving egg quality
CN103947875A (en) * 2014-04-15 2014-07-30 河南师范大学 Chinese medicinal feed additive and application thereof
CN104082530A (en) * 2014-06-13 2014-10-08 思南县常青种养专业合作社 Chinese herbal medicine additive for chicken feed
CN104187170A (en) * 2014-09-12 2014-12-10 安徽农业大学 Green tea composite chicken feed additive capable of enhancing immunity of chickens
CN104621408A (en) * 2015-02-12 2015-05-20 贵州省畜牧兽医研究所 Functional feed for improving immunity of laying hens and preparation method of functional feed
CN104839516A (en) * 2015-06-24 2015-08-19 福建亚森农业综合开发股份公司 Compound poultry feed
CN108310136A (en) * 2018-04-20 2018-07-24 佛山迁宇科技有限公司 A kind of feed addictive and preparation method thereof of prevention white diarrhea
CN109588572A (en) * 2019-01-30 2019-04-09 永胜腾飞家禽养殖有限公司 A kind of feed addictive of layer chicken improving egg quality

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