CN112220051A - 一种功能性胶原蛋白的其制备方法 - Google Patents
一种功能性胶原蛋白的其制备方法 Download PDFInfo
- Publication number
- CN112220051A CN112220051A CN202010891240.0A CN202010891240A CN112220051A CN 112220051 A CN112220051 A CN 112220051A CN 202010891240 A CN202010891240 A CN 202010891240A CN 112220051 A CN112220051 A CN 112220051A
- Authority
- CN
- China
- Prior art keywords
- collagen
- powder
- animal
- functional
- bone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 64
- 108010035532 Collagen Proteins 0.000 title claims abstract description 64
- 229920001436 collagen Polymers 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 45
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 20
- 241001465754 Metazoa Species 0.000 claims abstract description 19
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims abstract description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000013372 meat Nutrition 0.000 claims abstract description 6
- 239000002253 acid Substances 0.000 claims abstract description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 4
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 4
- 238000005238 degreasing Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims abstract description 4
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 229940111202 pepsin Drugs 0.000 claims abstract description 4
- 210000002435 tendon Anatomy 0.000 claims abstract description 4
- 235000020234 walnut Nutrition 0.000 claims description 10
- 244000061456 Solanum tuberosum Species 0.000 claims description 9
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 9
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims description 7
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims description 7
- 235000009685 Crataegus X maligna Nutrition 0.000 claims description 7
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims description 7
- 235000009486 Crataegus bullatus Nutrition 0.000 claims description 7
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims description 7
- 235000009682 Crataegus limnophila Nutrition 0.000 claims description 7
- 235000004423 Crataegus monogyna Nutrition 0.000 claims description 7
- 235000002313 Crataegus paludosa Nutrition 0.000 claims description 7
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims description 7
- 102000002322 Egg Proteins Human genes 0.000 claims description 6
- 108010000912 Egg Proteins Proteins 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000009496 Juglans regia Nutrition 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 235000018102 proteins Nutrition 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000758791 Juglandaceae Species 0.000 claims description 4
- 108010073771 Soybean Proteins Proteins 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- 230000002421 anti-septic effect Effects 0.000 claims description 3
- 238000000502 dialysis Methods 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 210000002969 egg yolk Anatomy 0.000 claims description 3
- 235000013345 egg yolk Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 210000003205 muscle Anatomy 0.000 claims description 3
- 235000012015 potatoes Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 235000019710 soybean protein Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 240000000171 Crataegus monogyna Species 0.000 claims 2
- 240000007049 Juglans regia Species 0.000 claims 2
- 241000718541 Tetragastris balsamifera Species 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 12
- 230000000694 effects Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241001092040 Crataegus Species 0.000 description 5
- 241000758789 Juglans Species 0.000 description 4
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 230000003020 moisturizing effect Effects 0.000 description 4
- 208000004998 Abdominal Pain Diseases 0.000 description 3
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
- 208000003351 Melanosis Diseases 0.000 description 2
- 208000019790 abdominal distention Diseases 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 206010000077 Abdominal mass Diseases 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 201000000736 Amenorrhea Diseases 0.000 description 1
- 206010001928 Amenorrhoea Diseases 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 208000002881 Colic Diseases 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010049077 Hernia pain Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010021746 Infantile colic Diseases 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- 206010024604 Lipoatrophy Diseases 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 206010040954 Skin wrinkling Diseases 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 231100000540 amenorrhea Toxicity 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229940001941 soy protein Drugs 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了一种功能性胶原蛋白的其制备方法，S1:选取高蛋白的动物品种，将先剔除动物骨上残留的肉质和肌腱等杂物；S2:利用粉碎机对动物骨进行粉碎，随后用正丁醇或正己烷萃取出骨油；S3:通过用稀酸或EDTA溶液除去骨中无机物以提高胶原蛋白的得率；S4:正乙烷脱脂后再用胃蛋白酶酶解，在加酶量150U/g，pH值1.7，然后在固液比1∶6的情况下抽提5h，在此条件下，提取率可达18％；S5:利用碱法对动物骨胶原白进行提取，得到胶原蛋白，随后进行烘干，得到胶原蛋白粉。本发明的有益效果是，本技术方案提供一种具有功能的胶原蛋白粉，该技术方案加入了多种果实，使得人体摄入后可有效的对胶原蛋白吸收，同时还能够提供更多的营养成分。
Description
技术领域
本发明涉及食品领域，特别是一种功能性胶原蛋白的其制备方法。
背景技术
胶原蛋白是生物高分子，动物***中的主要成分，也是哺乳动物体内含量最多、分布最广的功能性蛋白，占蛋白质总量的25％～30％，某些生物体甚至高达80％以上；
畜禽源动物组织是人们获取天然胶原蛋白及其胶原肽的主要途径，但由于相关畜类疾病和某些宗教信仰限制了人们对陆生哺乳动物胶原蛋白及其制品的使用，现今正在逐步转向海洋生物中开发。
发明内容
本发明的目的是为了解决上述问题，设计了一种功能性胶原蛋白的其制备方法。
实现上述目的本发明的技术方案为，一种功能性胶原蛋白的其制备方法，包括胶原蛋白10-20份、大豆分离蛋白粉32-38份、马铃薯蛋白粉0.6-1份、结晶果糖6-10份、核桃粉0.2-0.5份、蛋清粉0.5-0.8份以及蛋黄粉0.1-0.3份、山楂粉5-10粉。
一种功能性胶原蛋白的其制备方法，包含以下步骤：
S1:选取高蛋白的动物品种，将先剔除动物骨上残留的肉质和肌腱等杂物；
S2:利用粉碎机对动物骨进行粉碎，随后用正丁醇或正己烷萃取出骨油；
S3:通过用稀酸或EDTA溶液除去骨中无机物以提高胶原蛋白的得率；
S4:正乙烷脱脂后再用胃蛋白酶酶解，在加酶量150U/g，pH值1.7，然后在固液比1∶6的情况下抽提5h，在此条件下，提取率可达18％；
S5:利用碱法对动物骨胶原白进行提取，得到胶原蛋白，随后进行烘干，得到胶原蛋白粉；
S6：通过透析法对步骤S5中得到胶原蛋白进行提取；
S7；将新鲜大豆、去皮马铃薯、去壳核桃以及剥离籽后的山楂进行研磨成粉，得到果实粉；
S8：将果实粉进行灭菌处理，随后与胶原蛋白粉混合搅拌得到功能性的胶原蛋白粉；
S9：将产品进行密封处理，并加入防腐剂。
优选的，所述步骤S4中酶解时需37℃条件下处理120min。
优选的，所述步骤S2中破碎细度为10-20目。
优选的，所述步骤S7中的新鲜大豆、马铃薯以及核桃需要提前用水浸泡。
优选的，所述S7中的实粉研磨细度为60目以上，研磨机转速为45R/min。
利用本发明的技术方案制作的一种功能性胶原蛋白的其制备方法，本技术方案提供一种具有功能的胶原蛋白粉，该技术方案加入了多种果实，使得人体摄入后可有效的对胶原蛋白吸收，同时还能够提供更多的营养成分。
具体实施方式
一种功能性胶原蛋白的其制备方法。
一种功能性胶原蛋白，包括胶原蛋白10-20份、大豆分离蛋白粉32-38份、马铃薯蛋白粉0.6-1份、结晶果糖6-10份、核桃粉0.2-0.5份、蛋清粉0.5-0.8份以及蛋黄粉0.1-0.3份、山楂粉5-10粉。
大豆粉不但具有大豆蛋白质含量高，不饱和脂肪酸含量较高等特点，还具有抗衰老、健脑等保健功效，是一种营养价值较高的食物；
核桃中所含的精氨酸、油酸、抗氧化物质等对保护心血管，预防冠心病、中风、老年痴呆等是颇有裨益的；
山楂消食积，散瘀血，驱绦虫。治肉积，症瘕，痰饮，痞满，吞酸，泻痢，肠风，腰痛，疝气产后儿枕痛，恶露不尽，小儿乳食停滞。消食健胃，行气散瘀。用于肉食积滞、胃烷胀满、泻痢腹痛、瘀血经闭、产后瘀阻、心腹刺痛、疝气疼痛、高血脂症；
胶原蛋白由动物皮提取，皮中除胶原蛋白外还含有透明质酸、硫酸软骨素等蛋白多糖，它们含有大量极性基团，是保湿因子，且有阻止皮肤中的酪氨酸转化为黑色素的作用，故胶原蛋白有纯天然保湿、美白、防皱、祛斑等作用，可广泛应用于美容用品中。胶原蛋白的化学组成、结构赋予了它是美容的基础。胶原蛋白与人体皮肤胶原的结构相似，为非水溶性纤维状含糖蛋白质，分子中富含大量氨基酸和亲水基，具有一定的表面活性和很好的相容性，同时由于其分子中含有大量的羟基，因此它有着相当好的保湿作用。在相对湿度70％时，仍可保持其自身重量45％的水分。试验证明：0.01％的胶原蛋白纯溶液就能形成很好的保水层，供给皮肤所需要的全部水分。
随着年龄的增长，成纤维细胞的合成能力下降，若皮肤中缺乏胶原蛋白，胶原纤维就会发生联固化，使细胞间粘多糖减少，皮肤便会失去柔软、弹性和光泽，发生老化，同时真皮的纤维断裂、脂肪萎缩、汗腺及皮脂腺分泌减少，使皮肤出现色斑、皱纹等一系列老化现象。将其作为活性物质用于化妆品中时，后者可以扩散到皮肤的深层，其含有的酪氨酸与皮肤中的酪氨酸竞争，而与酪氨酸酶的催化中心结合，从而抑制黑色素的产生，使皮肤中的胶原蛋白活性增强，保持角质层水分以及纤维结构的完整性，促进皮肤组织的新陈代谢，对皮肤产生良好的滋润保湿、消皱美容作用。早在20世纪70年代初，美国就率先推出注射用牛胶原，用于祛斑除皱纹及修复瘢痕。
一种功能性胶原蛋白的其制备方法，包含以下步骤：
S1:选取高蛋白的动物品种，将先剔除动物骨上残留的肉质和肌腱等杂物；
S2:利用粉碎机对动物骨进行粉碎，随后用正丁醇或正己烷萃取出骨油；
S3:通过用稀酸或EDTA溶液除去骨中无机物以提高胶原蛋白的得率；
S4:正乙烷脱脂后再用胃蛋白酶酶解，在加酶量150U/g，pH值1.7，然后在固液比1∶6的情况下抽提5h，在此条件下，提取率可达18％；
S5:利用碱法对动物骨胶原白进行提取，得到胶原蛋白，随后进行烘干，得到胶原蛋白粉；
S6：通过透析法对步骤S5中得到胶原蛋白进行提取；
S7；将新鲜大豆、去皮马铃薯、去壳核桃以及剥离籽后的山楂进行研磨成粉，得到果实粉；
S8：将果实粉进行灭菌处理，随后与胶原蛋白粉混合搅拌得到功能性的胶原蛋白粉；
S9：将产品进行密封处理，并加入防腐剂。
作为优选方案，更进一步的，所述步骤S4中酶解时需37℃条件下处理120min。
作为优选方案，更进一步的，所述步骤S2中破碎细度为10-20目。
作为优选方案，更进一步的，所述步骤S7中的新鲜大豆、马铃薯以及核桃需要提前用水浸泡。
作为优选方案，更进一步的，所述S7中的实粉研磨细度为60目以上，研磨机转速为45R/min。
上述技术方案仅体现了本发明技术方案的优选技术方案，本技术领域的技术人员对其中某些部分所可能做出的一些变动均体现了本发明的原理，属于本发明的保护范围之内。
Claims (6)
1.一种功能性胶原蛋白，其特征在于，包括胶原蛋白10-20份、大豆分离蛋白粉32-38份、马铃薯蛋白粉0.6-1份、结晶果糖6-10份、核桃粉0.2-0.5份、蛋清粉0.5-0.8份以及蛋黄粉0.1-0.3份、山楂粉5-10粉。
2.根据权利要求1所述的一种功能性胶原蛋白的其制备方法，其特征在于，包含以下步骤：
S1:选取高蛋白的动物品种，将先剔除动物骨上残留的肉质和肌腱等杂物；
S2:利用粉碎机对动物骨进行粉碎，随后用正丁醇或正己烷萃取出骨油；
S3:通过用稀酸或EDTA溶液除去骨中无机物以提高胶原蛋白的得率；
S4:正乙烷脱脂后再用胃蛋白酶酶解，在加酶量150U/g，pH值1.7，然后在固液比1∶6的情况下抽提5h，在此条件下，提取率可达18％；
S5:利用碱法对动物骨胶原白进行提取，得到胶原蛋白，随后进行烘干，得到胶原蛋白粉；
S6：通过透析法对步骤S5中得到胶原蛋白进行提取；
S7；将新鲜大豆、去皮马铃薯、去壳核桃以及剥离籽后的山楂进行研磨成粉，得到果实粉；
S8：将果实粉进行灭菌处理，随后与胶原蛋白粉混合搅拌得到功能性的胶原蛋白粉；
S9：将产品进行密封处理，并加入防腐剂。
3.根据权利要求2所述的一种功能性胶原蛋白的其制备方法，其特征在于，所述步骤S4中酶解时需37℃条件下处理120min。
4.根据权利要求2所述的一种功能性胶原蛋白及其制备方法，其特征在于，所述步骤S2中破碎细度为10-20目。
5.根据权利要求2所述的一种功能性胶原蛋白的其制备方法，其特征在于，所述步骤S7中的新鲜大豆、马铃薯以及核桃需要提前用水浸泡。
6.根据权利要求2所述的一种功能性胶原蛋白的其制备方法，其特征在于，所述S7中的实粉研磨细度为60目以上，研磨机转速为45R/min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010891240.0A CN112220051A (zh) | 2020-08-30 | 2020-08-30 | 一种功能性胶原蛋白的其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010891240.0A CN112220051A (zh) | 2020-08-30 | 2020-08-30 | 一种功能性胶原蛋白的其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112220051A true CN112220051A (zh) | 2021-01-15 |
Family
ID=74116396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010891240.0A Withdrawn CN112220051A (zh) | 2020-08-30 | 2020-08-30 | 一种功能性胶原蛋白的其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112220051A (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104605225A (zh) * | 2015-01-02 | 2015-05-13 | 保龄宝生物股份有限公司 | 一种适合中老年人服用的胶原蛋白粉 |
| CN104770743A (zh) * | 2015-05-04 | 2015-07-15 | 威海御膳坊生物科技有限公司 | 一种复合蛋白粉及其制备方法 |
| CN109608540A (zh) * | 2019-01-29 | 2019-04-12 | 广州华大生物科技有限公司 | 一种从鱼鳞中提取胶原蛋白的方法 |
| CN109762865A (zh) * | 2019-03-25 | 2019-05-17 | 武汉轻工大学 | 一种牛骨胶原多肽的生产方法 |
-
2020
- 2020-08-30 CN CN202010891240.0A patent/CN112220051A/zh not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104605225A (zh) * | 2015-01-02 | 2015-05-13 | 保龄宝生物股份有限公司 | 一种适合中老年人服用的胶原蛋白粉 |
| CN104770743A (zh) * | 2015-05-04 | 2015-07-15 | 威海御膳坊生物科技有限公司 | 一种复合蛋白粉及其制备方法 |
| CN109608540A (zh) * | 2019-01-29 | 2019-04-12 | 广州华大生物科技有限公司 | 一种从鱼鳞中提取胶原蛋白的方法 |
| CN109762865A (zh) * | 2019-03-25 | 2019-05-17 | 武汉轻工大学 | 一种牛骨胶原多肽的生产方法 |
Non-Patent Citations (1)
| Title |
|---|
| 王晨等: "胃蛋白酶酶解提取鸡骨胶原蛋白工艺的研究", 《现代食品科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103340817B (zh) | 一种葛根美白面膜 | |
| CN109512691A (zh) | 一种蓝铜肽冻干粉制剂及其制备方法 | |
| KR102014680B1 (ko) | 염모제 조성물을 이용한 모발의 염색 시술방법 | |
| CN101343653A (zh) | 海参胶原蛋白活性肽的制备方法和应用 | |
| CN103565699B (zh) | 一种黑果枸杞祛斑美白抗衰老精华液及其制备方法 | |
| CN104490732B (zh) | 一种抗衰老鱼鳞胶原多肽面膜及其制备方法 | |
| CN108653013A (zh) | 一种植物保湿水润晒后修复霜及其制备方法 | |
| CN103585097A (zh) | 一种白及多糖载表皮生长因子复合物及其制备方法和应用 | |
| CN109627325A (zh) | 一种梯度胶原蛋白及胶原多肽分子的制备方法及其应用 | |
| CN108653006A (zh) | 一种植物保湿全效眼霜及其制备方法 | |
| CN108495616B (zh) | 用于改善皮肤皱纹的组合物以及利用该组合物用于改善皮肤皱纹的贴片和面膜 | |
| CN109381390A (zh) | 一种玻尿酸亮肤修护面膜及其制备方法 | |
| CN108888554B (zh) | 具有嫩肤淡斑的干细胞美容乳液及其制备方法 | |
| CN110810853A (zh) | 一种改善皮肤油水平衡、增加皮肤弹性的运动营养食品 | |
| CN110680775A (zh) | 一种美白保湿补水精粹液及其制备方法 | |
| CN112220051A (zh) | 一种功能性胶原蛋白的其制备方法 | |
| CN111067845A (zh) | 一种天然植物多效修复面膜及其制备方法 | |
| CN108653146A (zh) | 一种营养护手霜的制作方法 | |
| KR20030055950A (ko) | 석류 추출물을 함유하는 노화 방지용 화장료 조성물 | |
| CN111450035A (zh) | 纯天然美白丰胸和防治乳腺疾病的胸膜及其制备方法 | |
| CN111700818A (zh) | 一种祛皱抗衰人参多肽化妆水及其制备方法 | |
| CN1071816A (zh) | 一种美容保健食品的制备方法 | |
| CN108815107A (zh) | 一种颈膜及其制备方法 | |
| KR102374056B1 (ko) | 건강 개선을 위한 천연성의 조성물과 이를 포함한 기능성 샴푸제 및 화장제 | |
| CN110200840A (zh) | 一种具有抑菌、修复再生、补水保湿、延缓衰老作用的复合生物酶组合物 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210115 |