CN112189675A - Pesticide nano drug-loading system and preparation method thereof - Google Patents
Pesticide nano drug-loading system and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present disclosure relates to a pesticide nano drug-carrying system comprising nanoparticles and an active ingredient encapsulated within the nanoparticles; the nanoparticles are composed of a carrier material and auxiliary components, and the effective components comprise brassinolide, matrine and natural pyrethrin. Through the technical scheme, the pesticide nano drug delivery system provided by the disclosure has an obvious sustained and controlled release effect and can increase the drug duration; meanwhile, the pesticide nano drug-loading system has strong blade affinity, can be well attached to crop blades, and avoids the phenomenon that a large amount of pesticide drops enter the natural environment to pollute the natural environment, so that the pesticide nano drug-loading system disclosed by the invention reduces the dosage, improves the drug effect, and has obvious effects on relieving the environmental pollution caused by pesticide abuse, the drug resistance of pests, the loss of biological diversity and the like.
Description
Technical Field
The disclosure relates to the technical field of pesticide production, in particular to a pesticide nano drug delivery system and a preparation method thereof.
Background
The pesticide is an important resource for agricultural generation, and the application of the pesticide is an important means for preventing and controlling crop diseases and insect pests and regulating the growth of crops, and has important significance for the yield conservation and the yield increase of agricultural production. Most pesticides are unstable and easy to degrade under natural conditions, so that the pesticide has short duration and low utilization rate; moreover, the surfaces of most crop leaves have hydrophobic micro-nano structures such as wax layers, villi, mastoids and the like, pesticide liquid drops are difficult to attach to the crop leaves and fall off into the natural environment, the pesticide utilization rate is further reduced, meanwhile, the pesticide falling off into the natural environment causes environmental pollution, the health of human beings is threatened, and the pesticide resistance of harmful organisms is also induced. Therefore, it is necessary to develop a novel pesticide formulation to increase the pesticide effect, increase the amount of the pesticide adhering to the plant leaves, and reduce the amount of the pesticide used.
Disclosure of Invention
The purpose of the present disclosure is to provide a pesticide nano drug-carrying system and a preparation method thereof, wherein the pesticide nano drug-carrying system has obvious sustained and controlled release effects and leaf affinity effects.
In order to achieve the above objects, the present disclosure provides a pesticide nano drug-carrying system comprising nanoparticles and an active ingredient encapsulated within the nanoparticles; the nanoparticles are composed of a carrier material and auxiliary components, and the effective components comprise brassinolide, matrine and natural pyrethrin.
Optionally, in the effective component, relative to 1 weight part of brassinolide, the content of matrine is 0.1-40 weight parts, and the content of natural pyrethrin is 0.1-50 weight parts; preferably, the content of matrine is 1-10 parts by weight and the content of natural pyrethrin is 1-20 parts by weight relative to 1 part by weight of brassinolide.
Optionally, the average particle size of the pesticide nano drug delivery system is 69.7-142.5nm, the drug loading rate is 5.6-58.8%, and the encapsulation rate is 65.3-92.7%; preferably, the average particle size of the pesticide nano drug delivery system is 96.7-121.3nm, the drug loading rate is 19.6-33.5%, and the encapsulation rate is 76.3-88.5%.
Optionally, the carrier material comprises at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyethylene oxide-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer and polymethyl methacrylate; preferably, the carrier material is at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer and polymethyl methacrylate.
Optionally, the adjunct ingredients include an emulsifier and a wetting agent.
Alternatively, the auxiliary ingredient is used in an amount of 0.05 to 0.5 parts by weight, relative to 1 part by weight of the carrier material.
The present disclosure also provides a method for preparing the pesticide nano drug delivery system, wherein the method comprises:
a. dissolving the carrier material and the auxiliary component in an organic solvent to obtain a carrier solution;
b. dissolving the effective components in an organic solvent to obtain an effective component solution;
c. and mixing the carrier solution and the effective component solution, and stirring for 15-45min at the rotating speed of 240-360 rpm to obtain the nano drug delivery system.
Optionally, the organic solvent comprises at least one of dimethyl sulfoxide, acetone, ethyl acetate, N-octyl pyrrolidone, N-dimethylformamide, and N, N-dimethyldecanamide.
Alternatively, in step a, the organic solvent is used in an amount of 0.5 to 2.0 parts by weight, relative to 1 part by weight of the mixture of the support material and the auxiliary ingredient;
in the step b, the organic solvent is used in an amount of 0.5 to 2.5 parts by weight relative to 1 part by weight of the active ingredient.
Alternatively, in step c, the effective ingredient solution is used in an amount of 0.1 to 5 parts by volume relative to 1 part by volume of the carrier solution.
Through the technical scheme, the pesticide nano drug delivery system provided by the disclosure has an obvious sustained and controlled release effect and can increase the drug duration; meanwhile, the pesticide nano drug-loading system has strong blade affinity, can be well attached to crop blades, and avoids the phenomenon that a large amount of pesticide drops enter the natural environment to pollute the natural environment, so that the pesticide nano drug-loading system disclosed by the invention reduces the dosage, improves the drug effect, and has obvious effects on relieving the environmental pollution caused by pesticide abuse, the drug resistance of pests, the loss of biological diversity and the like.
Additional features and advantages of the disclosure will be set forth in the detailed description which follows.
Detailed Description
The following describes in detail specific embodiments of the present disclosure. It should be understood that the detailed description and specific examples, while indicating the present disclosure, are given by way of illustration and explanation only, not limitation.
A first aspect of the present disclosure provides a pesticide nano drug-carrying system comprising nanoparticles and an active ingredient encapsulated within the nanoparticles; the nanoparticles are composed of a carrier material and auxiliary components, and the effective components comprise brassinolide, matrine and natural pyrethrin.
In the technical scheme, the active ingredients are encapsulated in the nanoparticles, so that the problems of drug failure, low utilization rate and the like caused by the fact that the active ingredients are directly exposed in the natural environment and degraded can be avoided; meanwhile, the nanoparticles have slow release and controlled release effects, can continuously release effective components for a long time, and prolong the lasting period of the medicine; in addition, the nanoparticles also have blade affinity, can be well attached to crop blades, and prevent a large amount of pesticide droplets from entering the natural environment to pollute the natural environment. Therefore, the pesticide nano drug delivery system disclosed by the invention has the characteristics of low dosage, good drug effect and long lasting period, and is beneficial to relieving the problems of environmental pollution, pest drug resistance, biological diversity loss and the like caused by pesticide abuse.
Meanwhile, in the pesticide nano drug-carrying system, the effective components comprise brassinolide, matrine and natural pyrethrin, the three components have synergistic interaction, and compared with the single use of any one or two of the compositions, the combined use of the three components can more effectively repel pests, prevent and treat diseases, strengthen seedlings and promote the growth of regulator.
According to the present disclosure, the usage ratios of brassinolide, matrine and natural pyrethrin have synergistic effect in a wide range, and can reduce the dosage, reduce the application cost and reduce the pollution of pesticide residue to the environment. For example, in the active ingredient, the content of matrine can be 0.1-40 parts by weight and the content of natural pyrethrin can be 0.1-50 parts by weight relative to 1 part by weight of brassinolide; preferably, the matrine may be contained in an amount of 1-10 parts by weight and the natural pyrethrin may be contained in an amount of 1-20 parts by weight, relative to 1 part by weight of brassinolide. Within the specific preferable range, the synergism of the brassinolide, the matrine and the natural pyrethrin is more obvious, and the effects of repelling pests, preventing and treating diseases, strengthening seedlings, promoting regulator growth and the like aiming at crops are more obvious.
According to the present disclosure, the average particle size, the drug loading rate, the encapsulation rate, etc. of the pesticide nano drug-loading system can be changed in a large range, for example, the average particle size of the pesticide nano drug-loading system can be 69.7-142.5nm, the drug loading rate can be 5.6-58.8%, and the encapsulation rate can be 65.3-92.7%; preferably, the average particle size of the pesticide nano drug delivery system can be 96.7-121.3nm, the drug loading rate can be 19.6-33.5%, and the encapsulation rate can be 76.3-88.5%. Within the preferable range, the pesticide nano drug-carrying system has better sustained and controlled release effects.
According to the present disclosure, the carrier material constituting the above nanoparticles may be selected from a wide range, for example, the carrier material may include at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyethylene oxide-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer, and polymethyl methacrylate; preferably, the carrier material may be at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer, and polymethyl methacrylate. Under the optimal condition, the pesticide nano drug delivery system has better sustained-release and controlled-release effects and better leaf affinity.
The types of auxiliary ingredients that make up the nanoparticles described above can be selected within a wide range in accordance with the present disclosure, for example, the auxiliary ingredients can include emulsifying and wetting agents.
In accordance with the present disclosure, the relative amounts of carrier material and auxiliary ingredient in the above nanoparticles may vary within wide limits, for example, the auxiliary ingredient may be used in an amount of 0.05 to 0.5 parts by weight relative to 1 part by weight of the carrier material.
A second aspect of the present disclosure provides a method for preparing the pesticide nano drug delivery system described in any one of the above, the method comprising: a. dissolving the carrier material and the auxiliary component in an organic solvent to obtain a carrier solution; b. dissolving the effective components in an organic solvent to obtain an effective component solution; c. and mixing the carrier solution and the effective component solution, and stirring for 15-45min at the rotating speed of 240-360 rpm to obtain the nano drug delivery system.
Optionally, the organic solvent comprises at least one of acetone, ethyl acetate, N-octyl pyrrolidone, N-dimethylformamide, and N, N-dimethyldecanamide.
Alternatively, in step a, the organic solvent is used in an amount of 0.5 to 2.0 parts by weight, relative to 1 part by weight of the mixture of the support material and the auxiliary ingredient; in the step b, the organic solvent is used in an amount of 0.5 to 2.5 parts by weight relative to 1 part by weight of the active ingredient.
Alternatively, in step c, the effective ingredient solution is used in an amount of 0.1 to 5 parts by volume relative to 1 part by volume of the carrier solution.
The present disclosure is further illustrated by the following examples, but is not to be construed as being limited thereby. The raw materials, reagents, instruments and equipment involved in the examples of the present disclosure may be commercially available, unless otherwise specified.
Example 1
Mixing a carrier material (polyacrylamide-polylactic acid-glycolic acid block copolymer) and an auxiliary component (sodium dodecyl sulfate) according to a weight ratio of 1: 0.1, dissolving in an organic solvent (dimethyl sulfoxide) to obtain a carrier solution, wherein the amount of the organic solvent is 1 part by weight relative to 1 part by weight of the mixture of the carrier material and the auxiliary component; dissolving effective components (brassinolide, matrine and natural pyrethrin, wherein the dosage of matrine is 0.5 weight part and the dosage of natural pyrethrin is 1.5 weight part relative to 1 weight part of brassinolide) in an organic solvent (N, N-dimethyl capramide) to obtain an effective component solution, wherein the dosage of the organic solvent is 1 weight part relative to 1 weight part of the effective components; mixing the carrier solution and the effective component solution according to the volume ratio of 2.5: 1, stirring for 30min at the rotating speed of 300 revolutions per minute to obtain the pesticide nano drug-loading system of the embodiment.
Through detection, the average particle size of the pesticide nano drug delivery system prepared by the embodiment is 100.3nm, the drug loading rate is 25.6%, and the encapsulation rate is 85.5%.
Example 2
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: in the effective components, the dosage of matrine is 0.1 weight part and the dosage of natural pyrethrin is 50 weight parts relative to 1 weight part of brassinolide.
Example 3
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: in the effective components, the dosage of matrine is 40 weight parts and the dosage of natural pyrethrin is 0.1 weight part relative to 1 weight part of brassinolide.
Example 4
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: in the effective components, the dosage of matrine is 1 part by weight and the dosage of natural pyrethrin is 20 parts by weight relative to 1 part by weight of brassinolide.
Example 5
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: in the effective components, the dosage of matrine is 10 weight parts and the dosage of natural pyrethrin is 1 weight part relative to 1 weight part of brassinolide.
Example 6
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the carrier material used in this example was polymethyl methacrylate.
Example 7
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the carrier material used in this example was monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer.
Example 8
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the carrier material used in this example was a polyethylene glycol-polylactic acid-glycolic acid block copolymer.
Example 9
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the average particle size of the pesticide nano drug delivery system prepared by the embodiment is 69.7nm, the drug loading rate is 5.6%, and the encapsulation rate is 65.3%.
Example 10
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the average particle size of the pesticide nano drug delivery system prepared by the embodiment is 142.5nm, the drug loading rate is 58.8%, and the encapsulation rate is 92.7%.
Example 11
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the average particle size of the pesticide nano drug delivery system prepared by the embodiment is 96.7nm, the drug loading rate is 19.6%, and the encapsulation rate is 76.3%.
Example 12
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the average particle size of the pesticide nano drug delivery system prepared by the embodiment is 121.3nm, the drug loading rate is 33.5%, and the encapsulation rate is 88.5%.
Comparative example 1
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the active ingredient in example 1 was replaced with the same amount of brassinolide.
Comparative example 2
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the effective ingredient in example 1 was replaced with the same amount of matrine.
Comparative example 3
The method for preparing the pesticide nano drug-loaded system in the embodiment 1 is different from the method in the embodiment 1 in that: the effective ingredients in example 1 were replaced with the same amount of natural pyrethrin.
Comparative example 4
Mixing brassinolide, matrine and natural pyrethrin (the dosage of matrine is 0.5 weight part and the dosage of natural pyrethrin is 1.5 weight parts relative to 1 weight part of brassinolide), and preparing missible oil according to a conventional method in the field.
Comparative example 5
After brassinolide, matrine and natural pyrethrin are mixed (the dosage of matrine is 0.5 weight part and the dosage of natural pyrethrin is 1.5 weight part relative to 1 weight part of brassinolide), water dispersible granules are prepared according to the conventional method in the field.
Comparative example 6
Mixing brassinolide, matrine and natural pyrethrin (the dosage of matrine is 0.5 weight part and the dosage of natural pyrethrin is 1.5 weight part relative to 1 weight part of brassinolide), and preparing powder according to the conventional method in the field.
Test examples
The test example uses the pesticide preparations prepared in examples 1 to 12 and comparative examples 1 to 6 as test samples to test the control effect of the pesticide preparations on the fruit tip borer.
Test site: the test forest area is the Changbai mountain forest area of the Korean autonomous State of extended frontier of Jilin province, the soil fertility of the forest area is better, and the growth conditions of the trees are uniform. The test forest area belongs to a coniferous and broadleaf mixed forest, the average slope is 30 degrees, the red pine in the test forest area is used as a test object, the tree age of the red pine is more than 200 years, and the plant height is 25-30 m. About 15 pinus koraiensis are distributed in each mu of test forest area, about 70% of pinus koraiensis cones are subjected to insect pests, and 5-6 pests are distributed on each cone subjected to the insect pests.
The test method comprises the following steps: the experimental forest area is divided into 19 experimental plots, the area of each experimental plot is 50 mu, 18 experimental plots are used as experimental groups, the pesticide preparations prepared in examples 1-12 and comparative examples 1-6 are respectively applied, and the rest 1 experimental plot is used as a control group and is applied with clear water. In each test cell, the application amount of the pesticide preparations prepared in examples 1 to 12 and comparative examples 1 to 6 was 50mL, and the pesticide preparations were diluted 100 times with clear water and sprayed by a helicopter.
Respectively selecting 5 red pines in each test cell by adopting a five-point sampling method after 1 day, 7 days, 14 days and 21 days after the application of the pesticide, randomly selecting 5 red pine cones on each red pine, counting the number of the young leaf rollers in the cones, and calculating the control effect in each test cell, wherein the results are shown in table 1. The control effect of each test cell was calculated as follows:
control effect (%) - (reduction rate of population of insects in experimental group-reduction rate of population of insects in control group)/(reduction rate of population of insects in 1-control group) × 100
TABLE 1
As can be seen from table 1, the pesticide nano drug delivery system provided by the present disclosure has a good control effect on pine cone pests, and has a longer duration.
The preferred embodiments of the present disclosure have been described in detail above, however, the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present disclosure within the technical idea of the present disclosure, and these simple modifications all fall within the protection scope of the present disclosure.
It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention.
In addition, any combination of various embodiments of the present disclosure may be made, and the same should be considered as the disclosure of the present disclosure, as long as it does not depart from the spirit of the present disclosure.
Claims (10)
1. A pesticide nano drug-carrying system is characterized by comprising nanoparticles and an active ingredient encapsulated in the nanoparticles; the nanoparticles are composed of a carrier material and auxiliary components, and the effective components comprise brassinolide, matrine and natural pyrethrin.
2. The Nanocarpin delivery system according to claim 1, wherein the active ingredient comprises 0.1 to 40 parts by weight of matrine and 0.1 to 50 parts by weight of natural pyrethrin, based on 1 part by weight of brassinolide; preferably, the content of matrine is 1-10 parts by weight and the content of natural pyrethrin is 1-20 parts by weight relative to 1 part by weight of brassinolide.
3. The pesticide nano-drug delivery system of claim 1, which is characterized in that the mean particle size of the pesticide nano-drug delivery system is 69.7-142.5nm, the drug loading rate is 5.6-58.8%, and the encapsulation efficiency is 65.3-92.7%; preferably, the average particle size of the pesticide nano drug delivery system is 96.7-121.3nm, the drug loading rate is 19.6-33.5%, and the encapsulation rate is 76.3-88.5%.
4. The pesticide nano drug delivery system of claim 1, wherein the carrier material comprises at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyethylene oxide-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer and polymethyl methacrylate; preferably, the carrier material is at least one of monomethoxypolyethylene glycol-polylactic acid-glycolic acid block copolymer, polyacrylamide-polylactic acid-glycolic acid block copolymer and polymethyl methacrylate.
5. The pesticide nano drug delivery system of claim 1, wherein the adjuvant comprises an emulsifier and a wetting agent.
6. The pesticidal nanoparticie system of claim 5, wherein the adjuvant is used in an amount of 0.05-0.5 parts by weight relative to 1 part by weight of the carrier material.
7. A method for preparing the pesticide nano-drug delivery system of any one of claims 1 to 6, which is characterized by comprising the following steps:
a. dissolving the carrier material and the auxiliary component in an organic solvent to obtain a carrier solution;
b. dissolving the effective components in an organic solvent to obtain an effective component solution;
c. and mixing the carrier solution and the effective component solution, and stirring for 15-45min at the rotating speed of 240-360 rpm to obtain the nano drug delivery system.
8. The method of claim 7, wherein the organic solvent comprises at least one of acetone, ethyl acetate, N-octyl pyrrolidone, N-dimethylformamide, and N, N-dimethyldecanamide.
9. The process according to claim 7, wherein in step a, the organic solvent is used in an amount of 0.5 to 2.0 parts by weight, relative to 1 part by weight of the mixture of carrier material and auxiliary components;
in the step b, the organic solvent is used in an amount of 0.5 to 2.5 parts by weight relative to 1 part by weight of the active ingredient.
10. The method according to claim 9, wherein the effective ingredient solution is used in an amount of 0.1 to 5 parts by volume with respect to 1 part by volume of the carrier solution in step c.
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CN114342951A (en) * | 2021-12-09 | 2022-04-15 | 湖南农业大学 | Composite nanoparticle for accelerating degradation of pesticide residues and preparation method and application thereof |
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