CN114097773A - Suspending agent capable of preventing and controlling pine wood nematode disease - Google Patents

Suspending agent capable of preventing and controlling pine wood nematode disease Download PDF

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Publication number
CN114097773A
CN114097773A CN202010876921.XA CN202010876921A CN114097773A CN 114097773 A CN114097773 A CN 114097773A CN 202010876921 A CN202010876921 A CN 202010876921A CN 114097773 A CN114097773 A CN 114097773A
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pine
imidacloprid
fluopyram
suspending agent
monochamus alternatus
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吴松青
张飞萍
吴杰钦
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a suspending agent capable of preventing and controlling pine wood nematode disease, which comprises the following components in percentage by mass: 5-10% of fluopyram, 35-55% of imidacloprid, 8-30% of auxiliary agent, 0.1-1% of ethylene glycol monoundecyl ether and 5-52% of water. Two effective components of fluopyram and imidacloprid are mixed with wetting dispersion auxiliary agent and partial solvent by coarse crushing and superfine grinding, and then are fully mixed with other components to prepare the fluopyram-imidacloprid suspending agent. The population density of monochamus alternatus in the forest can be greatly reduced by imidacloprid contact killing, and meanwhile, the fluopyram has systemic property and can be conducted inside the pine trees to kill pine wood nematodes invading the pine trees. In addition, the addition of the auxiliary agent enables the active ingredients of the dosage form to be uniformly dispersed, avoids precipitation, has the functions of freezing resistance and defoaming, is beneficial to transportation and ensures the usability of the dosage form under the low-temperature condition, thereby playing the role of effectively preventing and controlling the pine wood nematode disease at different periods.

Description

Suspending agent capable of preventing and controlling pine wood nematode disease
Technical Field
The invention belongs to the field of chemical prevention and control of forestry diseases and insect pests, and particularly relates to a suspending agent capable of preventing and controlling pine wilt disease.
Background
The pine wood nematode disease is caused by pine wood nematode (pine wood nematode)Bursaphelenchus xylophilus) The pine wilt caused by the pine wilt disease can be died in a short time after the pine is infected with pine nematode disease, and the pine wilt disease has extremely strong spreading property, once the pine wilt disease occurs, the yield of pine forest can be reduced in a large area, great economic loss of forestry is caused, and the pine wilt disease is a destructive epidemic disease. The transmission of the pine wilt disease is mainly transmitted among plants by means of forest activities of a midhost monochamus alternatus besides artificial long-distance transportation of epidemic trees. In recent years, quarantine and control of trans-regional pine wood transportation by forestry law enforcement departments in various places are increasingly strict, so that the control of monochamus alternatus hope and pine wood nematodes becomes a main battlefield for preventing and controlling pine wood nematode diseases. The pine wood nematode parasitizes in pine resin canal of pine trees and blocks nutrition and moisture conduction of the pine trees, and the pesticide which is commonly used in agriculture and is effective to the soil nematode is difficult to act on the pine wood nematode. Adult monochamus alternatus feeding and breeding in forests, larvae are pine trunk borers, pine forest vegetation is dense, the traditional pesticide formulation and pesticide application mode are difficult to act on the adults and the larvae in the monochamus alternatus in the forests, the control effect on the monochamus alternatus is not ideal, and in addition, the problems of rain wash and the like are solved, the lasting period is short, and adverse effects are caused to the environment.
The prevention and control method of the pine wood nematode disease mainly comprises physical prevention and control, chemical prevention and control, biological prevention and control, forest control and trap control and the like, wherein the chemical prevention and control is used as a traditional prevention and control mode, the labor and material cost is low, the effect is fast, the action range is wide, and along with the continuous appearance of novel medicaments, the environment-friendly short versions of the novel prevention and control method are gradually supplemented, so that the novel prevention and control method plays an important role in the overall prevention and control of the pine wood nematode disease. The existing main pesticide formulations comprise powder, granules, wettable powder, soluble powder, water dispersible granules, suspending agents, missible oil, aqueous emulsion, soluble liquid, microcapsules and the like, the physical and chemical properties and the storage and transportation modes of the formulations are different, and different formulations can be selected according to different application requirements and conditions. In forestry, the operating conditions are more complicated compared with agriculture, and the requirements on medicaments, application modes and equipment are higher. The suspending agent is a pesticide formulation which appears in recent years, and highly dispersed viscous and flowable suspension is formed by grinding and refining solid raw pesticide, auxiliary agents such as wetting dispersant, viscosity regulator and the like and water. Suspending agent adds water and dilutes to certain multiple when using, uses the pharmaco-instrument such as unmanned aerial vehicle to spray in the woodland, is difficult for flying, can attach to the plant surface well, and is resistant to erode, and the period of validity is long. Compared with powder and granules, the suspending agent is more suitable for forestry application.
Pine wood nematodes parasitize inside the body of pine trees, and in order to act on pine wood nematodes, it is required that the agent must be absorbed by pine trees, i.e., have systemic properties. Fluopyram is a novel excellent nematicide appearing in recent years, is also a broad-spectrum bactericide, has the function of killing nematodes by inhibiting the activity of succinate dehydrogenase and interfering the respiration of the nematodes, and is a novel action mechanism. At present, fluopyram as an efficient nematicide and prophylactic agent is widely applied in agriculture, and has obvious effect in nematode control of vegetables and fruits such as tomatoes, bananas and the like. Meanwhile, researches show that the action effect of fluopyram on pine wood nematodes is remarkably higher than that of other nematicides.
The monochamus alternatus is the main propagation medium of the pine wood nematodes, and when adult worms of the monochamus alternatus eclosion and fly out, the monochamus alternatus can carry a large amount of pine wood nematodes and propagate the pine wood nematodes to a new plant when the new plant is eaten, so that the pine wood nematodes are rapidly diffused. At present, the monochamus alternatus trapper widely used nationwide greatly reduces the population density of monochamus alternatus and has obvious effect of preventing and treating pine wilt disease. However, since monochamus alternatus responds to attractant stimuli only for a short specific period after adult eclosion, many monochamus alternatus have already spread to pine wood nematodes before they are trapped by the trap. The chemical prevention and control are not limited by the physiological stage of Monochamus alternatus, the lethal speed is high, and the prevention and control efficiency is high. Imidacloprid is a nitromethylene heterocyclic compound which can form antagonism with acetylcholine receptor, thus achieving poisoning effect by influencing the function of nervous system of insects. The imidacloprid is used as a high-efficiency systemic insecticide with broad spectrum, low toxicity, long residual effect period, environmental protection, no cross resistance, strong contact killing capability, quick response and wide application in agriculture. Experiments show that the imidacloprid has good contact killing performance on Monochamus alternatus.
At present, most of common agents for preventing and treating the pine wood nematode disease have drug effect only on one of the pine wood nematode disease or monochamus alternatus disease, and the common agents are fresh and effective on the pine wood nematode disease and the monochamus alternatus disease simultaneously.
Disclosure of Invention
The invention aims to provide a compound suspending agent which has attractive attraction capacity on monochamus alternatus and has contact toxicity while preventing the pine wilt disease, and before the pine wilt disease occurs, pine forests are sprayed to kill nematodes in pine bodies of infection sources, so that a transmission path of monochamus alternatus is blocked, and healthy plants have resistance.
In order to achieve the purpose, the invention adopts the following technical scheme:
the suspending agent capable of preventing and controlling the pine wilt disease comprises the following components in percentage by mass: 5-10% fluopyram; 35-55% imidacloprid; 8-30% of an auxiliary agent; 0.1-1% monochamus alternatus attractant; 5-62% of water.
The auxiliary agent comprises one or more of a wetting dispersant, an adhesive, an antifreezing agent, a defoaming agent, a preservative and a stabilizer.
The wetting dispersant comprises but is not limited to one or more of saponin, nekal, modified phosphate ester salt, sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, calcium dodecyl benzene sulfonate, sodium lignin sulfonate, sodium methylene dinaphthalene sulfonate, sodium naphthalene sulfonate formaldehyde condensate and sodium lauryl sulfate, and preferably modified phosphate ester salt and sodium lignin sulfonate.
The adhesive includes, but is not limited to, one or more of urea, sodium alginate, xanthan gum, arabic gum, magnesium aluminum silicate, cellulose methyl compound, polyacrylate, phenolic resin, polyethylene glycol and polyvinyl alcohol, preferably xanthan gum and polyvinyl alcohol.
The antifreeze includes but is not limited to one or more of ethylene glycol, polyethylene glycol, propylene glycol, glycerol, sodium chloride and urea, and preferably ethylene glycol and urea.
The defoaming agent comprises but is not limited to one or more of soybean oil, rapeseed oil, glycerol, fatty alcohol, polyoxypropylene glycerol ether, silicone oil, silicone, isooctanol, glycerol trihydrocarbon polyether, phenethyl alcohol oleate and phenethyl alcohol laurate, and preferably silicone oil and silicone.
The preservative comprises but is not limited to one or more of benzoic acid, benzoate, sorbic acid, sorbate, sodium diacetate, sodium deoxyacetate, calcium propionate, hydrogen peroxide and propyl p-hydroxybenzoate, and is preferably benzoate and sorbate.
The stabilizer includes but is not limited to one or more of phosphorous acid, alkyl phosphate, alkyl phosphite, triphenyl phosphite, epoxidized soybean oil and epichlorohydrin, and is preferably phosphorous acid and epichlorohydrin.
The monochamus alternatus attractant is ethylene glycol monoundecyl ether.
The components in the proportion are preferably as follows: 10% fluopyram; 45% imidacloprid; 15.2% of an auxiliary agent; 1% monochamus alternatus attractant; 28.8% water. The auxiliary agent comprises 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent and 3% of antifreezing agent.
The production process of the suspending agent comprises the following steps:
(1) coarsely crushing and mixing fluopyram, imidacloprid, monochamus alternatus attractant, wetting dispersant, defoaming agent and part of water to form a coarse mixture;
(2) carrying out superfine grinding on the coarse mixture until the particle size is 0.5-5 mu m to form effective liquid;
(3) mixing and dissolving the monochamus alternatus attractant with the rest of the auxiliary agent and water to form a regulating solution;
(4) the effective liquid and the regulating liquid are fully mixed to form the finished product of the compound suspending agent 'fluroxypyr imidacloprid'.
The components and the proportion of the rest of the auxiliary agents are selected according to different auxiliary agent costs and are limited by production conditions, can be flexibly adjusted, are known to persons skilled in the art, are protected by the patent and are not specifically described.
The application of a pine wood nematode disease control agent simultaneously having toxicity on Monochamus alternatus comprises the following steps:
(1) the suspending agent is diluted by water to be 500-800 times of liquid, an agricultural unmanned aerial vehicle is used, the suspending agent is sprayed above pine forests in a prevention mode, and the reference dosage per mu is 1 liter. Can kill pine wood nematode which is not erupted in pine trees, and kill Monochamus alternatus which is a transmission medium.
(2) Before the pine wilt disease occurs, the suspending agent is diluted to 1000-2000 times of liquid by water, and a backpack type medicine instrument is used for spraying in a forest or irrigating the roots of plants, wherein the reference dosage per mu is 30 liters. Can kill pine wood nematode which is not erupted in pine trees, and kill Monochamus alternatus which is a transmission medium.
(3) After the pine wood nematode disease is developed, the suspending agent is diluted into 1000 times of liquid by water, and is sprayed in a forest by using a backpack type pesticide instrument, wherein the reference dosage per mu is 30 liters. Killing monochamus alternatus in forests and blocking the channel for the pine wood nematodes to continuously spread. The diluent can also be used for treating dead wood, killing pine wood nematodes in epidemic trees and reducing infection sources.
Compared with the prior art, the invention has the following advantages:
(1) the suspending agent can kill the pine wood nematodes hidden in an infection source before the pine wood nematodes are attacked, and has insecticidal toxicity to Monochamus alternatus which is a transmission medium of the pine wood nematodes. The novel efficient nematicide fluopyram adopted by the medicament can influence the metabolism of succinate dehydrogenase in the pine wood nematode, thereby inhibiting the respiration of the pine wood nematode and achieving the purpose of nematocidal, is a novel nematocidal mechanism and has low toxicity to users and environment. Meanwhile, imidacloprid, another active ingredient of the pesticide, is a systemic pesticide widely applied in agriculture, has broad-spectrum insecticidal property, can influence the nervous system function of target pests, thereby quickly killing the pests, having high efficiency and low toxicity, being difficult to generate resistance and having good insecticidal activity on coleoptera insects such as Monochamus alternatus and the like. The pesticide adopts a suspending agent formulation, is convenient to use, is used in forestry, and has the characteristics of strong adhesion and difficult flying compared with formulations such as powder, and the like, thereby being more beneficial to the exertion of the effect.
(2) On the basis of simultaneously preventing and controlling monochamus alternatus and pine wood nematodes, the addition of the auxiliary agent monochamus alternatus attractant improves the insecticidal capacity of the mixed drug and enlarges the prevention and control effect on pine wood nematode to a great extent.
Detailed Description
Example 1: "Fluopyrazine-imidacloprid" field prevention
The formula is as follows: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent, 3% of antifreezing agent, 1% of ethylene glycol monoundecyl ether and 28.8% of water.
Auxiliary agent: the wetting dispersant is sodium lignosulphonate, so that the wettability of the effective components in the solvent can be increased, and a good initial dispersing effect is achieved; the adhesive is xanthan gum, so that the effect of preventing the suspending agent from being layered can be achieved, and meanwhile, the adhesive has a better adsorption effect when being applied in forests, so that the pesticide effect has higher durability; the defoaming agent is silicone oil, so that a large amount of foam generated during preparation, transportation and dilution of the suspending agent is reduced, and the use of the agent is influenced; the antifreezing agent is glycol, which lowers the freezing point of the suspending agent solvent, so that the agent can be normally used under the low-temperature condition.
The preparation method comprises the following steps:
(1) coarsely crushing and mixing 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoamer and 20% of water to form a coarse mixture;
(2) carrying out superfine grinding on the coarse mixture until the particle size is 2 mu m to form effective liquid;
(3) 4% of adhesive, 3% of antifreezing agent, 1% of ethylene glycol mono-undecyl ether and 8.8% of water are mixed to form a regulating solution;
(4) the effective liquid and the regulating liquid are fully mixed to form the finished product of the compound suspending agent 'fluroxypyr imidacloprid'.
The experimental method comprises the following steps: 20 three-year-old masson pine seedlings with good growth vigor and similar indexes such as plant height, basal diameter and the like are divided into two groups, one group is used as an experimental group, the 1500-fold diluted solution of the fluroxypyr imidacloprid suspending agent is sprayed, and the other group is used as a control group, and the same amount of clear water is sprayed. After one week, two groups of seedlings were inoculated with nematodes, 3000 pine wood nematodes were inoculated per plant, and the disease was continuously observed and recorded as follows:
table 1: preventing effect of 'Fluopyr imidacloprid' field masson pine seedlings
Figure 373944DEST_PATH_IMAGE001
Note: degree of onset: no disease, green plants; + first stage, color change of partial needle leaves; + two stages; most of the needles discolor; and the color of the whole plant is changed to wither and death by + and + three stages.
Example 2: 'Fluopyr imidacloprid' monochamus alternatus forest control
The formula is as follows: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent, 3% of antifreezing agent, 1% of ethylene glycol monoundecyl ether and 28.8% of water.
The auxiliaries are as in example 1.
The preparation method comprises the following steps:
(1) coarsely crushing and mixing 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoamer and 20% of water to form a coarse mixture;
(2) carrying out superfine grinding on the coarse mixture until the particle size is 3 mu m to form effective liquid;
(3) 4% of adhesive, 3% of antifreezing agent, 1% of ethylene glycol mono-undecyl ether and 8.8% of water are mixed to form a regulating solution;
(4) the effective liquid and the regulating liquid are fully mixed to form the finished product of the compound suspending agent 'fluroxypyr imidacloprid'.
The experimental method comprises the following steps:
in the woodland of the bursaphelenchus xylophilus disease area, 60 mu of sample land is selected, 3 traps are hung, the sample land is repeated for 3 times, the pesticide application time is 1-3 days in 7 months, the number of monochamus alternatus hopes in the traps in the 6 months of the year is counted before the suspending agent is applied, the number of monochamus alternatus hopes in the 6 months of the sample land in the second year after pesticide application is counted, and the woodland of the same area except 100 meters of each pesticide application sample land is used as a control, and 3 sample lands are used.
The statistical time of the trapper monochamus alternatus is 6 months to a whole month, the trapper monochamus alternatus is hung on a mountain top, a forest edge, a wider area in a forest or branches at the lower layer of pine trees, the lower end of the trapper needs to be above l.5 m away from the ground, the trapper is hung as high as possible under the condition, and the trapper monochamus alternatus is arranged in the forest in a triangular shape or a grid shape. When the edge of the forest stand is hung, the edge is hung in a small class and is more than 150 m away from the edge. Trap is rated at 2 hm2The left and right sides are hung at a density of 1 piece, and the distance is 100-150 m. During hanging, the trap needs special management to prevent artificial and extreme weather damage, and meanwhile, the trap quantity is counted periodically, the trap core or the attractant is replaced (added), and dry branches and fallen leaves in the trap are cleaned.
TABLE 2. number of Monochamus alternatus Hope in-plot traps
Figure 229773DEST_PATH_IMAGE002
From the table 2, the reduction rate of the Monochamus alternatus population in the application region can be calculated to be 67.57%, while the number of the Monochamus alternatus populations which are not applied is slightly increased, and the suspending agent has an obvious control effect on the Monochamus alternatus population.
Example 3: forest control of "Fluopyrazine imidacloprid" pine wood nematode disease
The formula is as follows:
10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent, 3% of antifreezing agent, 1% of ethylene glycol monoundecyl ether and 28.8% of water.
The auxiliaries are as in example 1.
The preparation method comprises the following steps:
(1) coarsely crushing and mixing 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoamer and 20% of water to form a coarse mixture;
(2) carrying out superfine grinding on the coarse mixture until the particle size is 1 mu m to form effective liquid;
(3) 4% of adhesive, 3% of antifreezing agent and 1% of monochamus alternatus attractant are mixed with the rest water to form a regulating solution;
(4) the effective liquid and the regulating liquid are fully mixed to form the finished product of the compound suspending agent 'fluroxypyr imidacloprid'.
The experimental method comprises the following steps:
and 5, 30-31 days in a forest land of the pine wood nematode disease area, taking 30 mu of land for each masson pine forest experiment, wherein the average diameter at breast height of the masson pine is about 15cm, the height of the masson pine is about 12m, and the degree of closure is about 0.7-0.8. The prepared pesticide suspending agent is used at the concentration of 100ml/hm2And spraying the masson pine forest by using an unmanned aerial vehicle, wherein the three tests are repeated, and the area is 90 mu in total. The forest land without pesticide application is used as a control, the test method is the same, three plots are used, and the total area is 90 mu. And counting the death condition of the newly added masson pine after 6 months.
Figure 160820DEST_PATH_IMAGE003
TABLE 3 suspending agent control
Figure 600635DEST_PATH_IMAGE004
After the same column of data, different lower case letters indicated significant differences (p < 0.05) and different upper case letters indicated significant differences (p < 0.01).
The number of pinus massoniana in the forest in the test area is respectively 216, 303 and 336, and the average growth rate of the died; the number of pinus massoniana in the forest of the untreated control forest stand is 240, 187 and 315 respectively, and the average growth rate of the died epidemic trees is 4.40%. The above results show that: the growth rate of the withered and dead epidemic wood can be reduced by spraying the fluopyram suspending agent. In addition, statistical analysis shows that the average prevention effect of the fluopyram suspending agent on the pine wilt disease can reach 78.63%.
Under the comparison of the number of monochamus alternatus hopes and the number of dead and dead trees in a forest, the suspending agent disclosed by the invention has an obvious effect of preventing and treating the pine wilt disease.
Example 4: woodland prevention and control of pine wilt disease by different 'fluroxypyr imidacloprid' suspending agents
The formula is as follows:
suspending agent 1: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent, 3% of antifreezing agent, 1% of ethylene glycol monoundecyl ether and 28.8% of water.
Suspending agent 2 contains no attractant: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 4% of adhesive, 0.2% of defoaming agent, 3% of antifreezing agent and 29.8% of water.
Suspension 3 contains no binder: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoaming agent, 3% of antifreezing agent, 1% of Monochamus alternatus attractant and 32.8% of water.
Suspending agent 4 contains no attractant and no adhesive: 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoaming agent and 33.8% of water.
The auxiliaries are as in example 1.
The preparation method comprises the following steps:
(1) coarsely crushing and mixing 10% of fluopyram, 45% of imidacloprid, 8% of wetting dispersant, 0.2% of defoamer and 20% of water to form a coarse mixture;
(2) carrying out superfine grinding on the coarse mixture until the particle size is 1 mu m to form effective liquid;
(3) 4% of adhesive, 3% of antifreezing agent and 1% of ethylene glycol mono-undecyl ether are mixed with the rest water to form a regulating solution;
(4) the effective liquid and the regulating liquid are fully mixed to form the finished product of the compound suspending agent 'fluroxypyr imidacloprid'.
The experimental method comprises the following steps:
taking 30 mu of land for each masson pine forest experiment in the forest land of the pine wilt disease epidemic area within 6 months, 20 days to 22 days, wherein the average diameter at breast height of the masson pine is about 15cm, the height of the masson pine is about 12m, and the degree of closure is about 0.7 to 0.8. The prepared pesticide suspending agent is used at the concentration of 100ml/hm2And spraying the masson pine forest by using an unmanned aerial vehicle, wherein the three tests are repeated, and the area is 90 mu in total. In addition, the same effective component agents without attractant, adhesive and attractant and adhesive are respectively tested, and the method is the same as that of the suspending agent. The forest land without pesticide application is used as a control, the test method is the same, three plots are used, and the total area is 90 mu. In 6 monthsAnd then counting the death condition of the newly added masson pine.
Control effect (%) = [ (number of dead plants in blank control area-number of dead plants in treatment area)/number of dead plants in blank control area ]. times.100%
TABLE 4 suspending agent control
Figure 275330DEST_PATH_IMAGE005
After the same column of data, different lower case letters indicated significant differences (p < 0.05) and different upper case letters indicated significant differences (p < 0.01).
According to the statistics of the number of newly added dead wood in table 3, 14 newly added dead wood, 19 newly added dead wood in the test area without attractant, 21 newly added dead wood in the test area without adhesive, 25 newly added dead wood in the test area without attractant and adhesive and 57 newly added dead wood in the control area are found in the application area of the suspending agent of the invention, and compared with the test area without additive, the number of dead wood in the application area of the suspending agent of the invention is obviously reduced. The control effect of the pine wilt disease of the suspending agent application area of the invention can be calculated to be 75.43%, and the control effect is obvious compared with the control effect of the suspending agent with the same effective component without the addition of the auxiliary agent, which shows that the added adhesive and attractant have synergistic effect on the use of fluopyram and imidacloprid.
The present invention is described in detail with reference to the above embodiments. It should be noted that the above embodiments are only for illustrating the present invention. Numerous alternatives and modifications can be devised by those skilled in the art without departing from the spirit and scope of the invention, which should be construed as within the scope of the invention.

Claims (10)

1. The suspending agent capable of preventing and controlling the pine wilt disease is characterized by comprising the following components in percentage by mass: 5-10% of fluopyram, 35-55% of imidacloprid, 8-30% of auxiliary agent, 0.1-1% of monochamus alternatus attractant and 5-52% of water.
2. The suspension concentrate of claim 1, wherein the adjuvant comprises one or more of a wetting dispersant, an adhesive, an antifreeze, an antifoaming agent, a preservative, and a stabilizer.
3. The suspension concentrate of claim 2, wherein the wetting dispersant is one or more of modified phosphate ester salts, sodium lignosulfonate, sodium lauryl sulfate, and the like.
4. A suspension as claimed in claim 2 wherein said binder is one or more of xanthan gum, acacia, magnesium aluminium silicate, cellulose methyl compound and the like.
5. The suspension concentrate of claim 2, wherein the anti-freeze is one or more of ethylene glycol, urea, polyethylene glycol, and the like.
6. The suspension concentrate of claim 2, wherein the defoamer is one or more of silicone oil, silicone, glycerin, and the like.
7. The suspension concentrate of claim 2, wherein the preservative is one or more of benzoate, sorbate, sodium diacetate, and the like.
8. The suspension concentrate according to claim 2, characterized in that the stabilizer is one or more of phosphorous acid, alkyl phosphate, epichlorohydrin, etc.
9. A suspension concentrate as claimed in claim 1, wherein the monochamus alternatus attractant is ethylene glycol monoundecyl ether.
10. Use of the suspension concentrate of claim 1 for the control of bursaphelenchus xylophilus and Monochamus alternatus.
CN202010876921.XA 2020-08-27 2020-08-27 Suspending agent capable of preventing and controlling pine wood nematode disease Pending CN114097773A (en)

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CN111011375A (en) * 2019-11-11 2020-04-17 南京生兴有害生物防治技术股份有限公司 Novel preparation method of compound monochamus alternatus attractant and lure

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WO2013180292A1 (en) * 2012-05-31 2013-12-05 国立大学法人 琉球大学 Control agent and control method for pine wilt
KR20160099477A (en) * 2015-02-12 2016-08-22 대구대학교 산학협력단 Trapping agent of monochamus alternatus and method for manufacturing the same and method for controlling bursaphelenchus xylophilus using the same
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CN114617013A (en) * 2022-03-25 2022-06-14 安榆林 Method for preventing and controlling pine wilt disease

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