CN112175785B - Dual-channel isothermal amplification device and method - Google Patents
Dual-channel isothermal amplification device and method Download PDFInfo
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- CN112175785B CN112175785B CN201910588093.7A CN201910588093A CN112175785B CN 112175785 B CN112175785 B CN 112175785B CN 201910588093 A CN201910588093 A CN 201910588093A CN 112175785 B CN112175785 B CN 112175785B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Abstract
The invention discloses a two-channel isothermal amplification device and a method, wherein the device comprises the following components: the first substrate, the first ion selective electrode, the second substrate, the second ion selective electrode and the third substrate are sequentially arranged from bottom to top; the device comprises a first electric signal leading-out connecting piece, a second electric signal leading-out connecting piece, an electric signal acquisition circuit board and a processor. The amplification result of the isothermal amplification technology is detected by adopting an electric signal of the sample collected by the ion selective electrode, so that the amplification result can be accurately and rapidly judged; two samples can be detected simultaneously by setting two channels for isothermal amplification, and one sample can be set as a blank comparison sample so as to be convenient for comparison with the other sample, thereby reducing misjudgment of detection results; and the ion selective electrode is small and exquisite, the manufacturing process is simple, the cost is low, thus the two-channel isothermal amplification device is also small and exquisite, the carrying and the storage are convenient, and the detection cost of the amplification result is also low.
Description
Technical Field
The invention relates to the field of detection equipment, in particular to a two-channel isothermal amplification device and method.
Background
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology which has been widely used for detection of various pathogen genes, such as influenza virus, norovirus, coronavirus, tubercle bacillus, etc., due to its good detection specificity, high sensitivity, short detection time and no need of thermal cycling equipment.
Currently, the detection methods for the amplification result of LAMP amplification technology mainly include a turbidity method and a luciferase method. The luciferase method needs to judge the amplification result by observing white sediment or green fluorescence after the LAMP amplification reaction is finished, and cannot monitor the progress of the reaction in real time, so that uncertainty is generated in the detection time of pathogens, and the efficiency is low; although the turbidity method can monitor the whole reaction process in real time and judge the result according to the existence of a turbidity curve, the turbidity method relies on expensive equipment, so that the detection cost of the LAMP amplification technology is greatly increased.
Disclosure of Invention
In order to overcome the defects of the detection method of the amplification result of the LAMP amplification technology, the embodiment of the invention provides a double-channel isothermal amplification device and a double-channel isothermal amplification method.
According to a first aspect, an embodiment of the present invention provides a dual-channel isothermal amplification device, including: the first substrate, the first ion selective electrode, the second substrate, the second ion selective electrode and the third substrate are sequentially arranged from bottom to top; the first substrate comprises a first heating device for carrying and heating a first ion selective electrode; the first ion selective electrode is used for bearing a first sample and collecting a first electric signal output by the first sample; a second substrate for carrying a second ion-selective electrode; a third substrate including a second heating means for heating the second ion-selective electrode; the second ion selective electrode is used for bearing a second sample and collecting a second electric signal output by the second sample; the first electric signal extraction connecting piece is connected with the first ion selective electrode and is used for extracting the first electric signal acquired by the first ion selective electrode; the second electric signal extraction connecting piece is connected with the second ion selective electrode and is used for extracting a second electric signal acquired by the second ion selective electrode; the electric signal acquisition circuit board is respectively connected with the first electric signal extraction connecting piece and the second electric signal extraction connecting piece and is used for acquiring a first electric signal extracted by the first electric signal extraction connecting piece and a second electric signal extracted by the second electric signal extraction connecting piece; and the processor is connected with the electric signal acquisition circuit board and used for judging whether the first sample and the second sample are amplified or not according to the first electric signal and the second electric signal.
Optionally, the first heating device comprises a first heating plate and the second heating device comprises a second heating plate.
Optionally, the first heating device and the second heating device are in communication.
Optionally, the first ion-selective electrode is any one of a hydrogen ion-selective electrode, a sodium ion-selective electrode, a potassium ion-selective electrode, a chloride ion-selective electrode, and a calcium ion-selective electrode; the second ion-selective electrode is any one of a hydrogen ion-selective electrode, a sodium ion-selective electrode, a potassium ion-selective electrode, a chloride ion-selective electrode, and a calcium ion-selective electrode.
Optionally, the first ion-selective electrode and/or the second ion-selective electrode comprises: the device comprises a basal layer, a measuring electrode layer, a reference electrode layer, an insulating layer, a solid electrolyte layer of the measuring electrode, a solid electrolyte layer of the reference electrode, a gas permeable layer of the measuring electrode, a gas permeable layer of the reference electrode, a bonding layer, a sample injection layer and a sealing film which are arranged in a laminated manner; wherein the measuring electrode layer and the reference electrode layer are positioned on the same layer, the solid electrolyte layer of the measuring electrode and the solid electrolyte layer of the reference electrode are positioned on the same layer, and the ventilation layer of the measuring electrode and the ventilation layer of the reference electrode are positioned on the same layer; the gas permeable layer of the reference electrode is provided with a first area positioned on one side of the solid electrolyte layer of the reference electrode away from the reference electrode layer and a second area positioned on the periphery of the first area; the second region is provided with an opening for communicating the solid electrolyte layer of the reference electrode with the outside.
Optionally, a first opening is formed in the insulating layer to allow the reference electrode layer to contact the solid electrolyte layer of the reference electrode; and a second opening is arranged on the insulating layer to enable the measuring electrode layer to be in contact with the solid electrolyte layer of the measuring electrode.
Optionally, the material of the sample injection layer is polyester fiber.
Alternatively, the solid electrolyte layer of the reference electrode is formed by mixing gelatin with a saturated chloride ion solution and then drying.
Optionally, the reference electrode layer comprises a conductive layer and a silver chloride layer in a stacked arrangement.
According to a second aspect, an embodiment of the present invention provides a two-channel isothermal amplification method, which is applied to the two-channel isothermal amplification device in the first aspect and any embodiment of the first aspect, and includes: placing the first sample and the second sample on the first ion-selective electrode and the second ion-selective electrode, respectively; heating the first ion-selective electrode and the second ion-selective electrode by a first heating device and a second heating device, respectively; collecting a first electric signal and a second point signal output by a first sample and a second sample respectively by using a first ion selective electrode and a second ion selective electrode; and judging whether the first sample and the second sample are amplified or not according to the first electric signal and the second electric signal by the processor.
The embodiment of the invention has the beneficial effects that:
(1) The amplification result of the isothermal amplification technology can be accurately and rapidly judged by adopting the electric signal of the sample collected by the ion selective electrode to detect the amplification result of the isothermal amplification technology; two samples can be detected simultaneously by setting two channels for isothermal amplification, and one sample can be set as a blank comparison sample so as to be convenient for comparison with the other sample, thereby reducing misjudgment of detection results; the ion selective electrode has simple manufacturing process and low cost, so that the detection cost of the amplification result of the isothermal amplification technology is low; and the ion selective electrode is small, so that the two-channel isothermal amplification device is small, and is convenient to carry and store.
(2) The ion selective electrode adopts a solid reference electrode, and the solid reference electrolyte in the solid reference electrode has the property of converting ion conduction into electron conduction, is arranged in the reference electrode to replace electrolyte solution, so that the solid reference electrode is obtained, the tolerance of the reference electrode to the environment is improved, and the ion selective electrode is suitable for being soaked in a liquid detection environment and is easy to store and use. The solid electrolyte layer of the reference electrode transfers electrons to the reference electrode layer, generating a reference potential, and the concentration of ions in the solid electrolyte layer of the reference electrode is fixed, so that a constant reference potential can be obtained. When the solid reference electrode is used for detecting a sample to be detected, the opening which exposes the solid electrolyte layer of the reference electrode is formed in the reference electrode film, so that water vapor in the sample enters the solid electrolyte layer of the reference electrode through the opening, the solid electrolyte in the sample is dissolved, the time required for generating liquid ions in the solid electrolyte layer is effectively shortened, and the time required for the solid reference electrode to reach a stable potential is greatly shortened. Meanwhile, the opening is arranged in the second area of the reference electrode film, so that the surface of the first solid electrolyte layer, which is in contact with the sample to be detected, is avoided, and ions in the detection environment can be prevented from entering the solid reference electrode through the opening while the water vapor transmission is accelerated, the interference of environmental factors is reduced, and the stability of the solid reference electrode is maintained. The time required for the solid reference electrode to reach the stable potential is short, so that the ion selective electrode can realize rapid detection of target parameters; on the other hand, the solid reference electrode has small potential fluctuation and high potential stability along with time, so that the ion selective electrode has high detection stability on the detection of target parameters.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 shows a schematic structure of a dual-channel isothermal amplification device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram showing the structure of an ion-selective electrode according to an embodiment of the present invention;
fig. 3 shows a schematic diagram of an electrical signal collected by a processor according to an embodiment of the invention.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention.
The embodiment of the invention provides a dual-channel isothermal amplification device, as shown in fig. 1, comprising: the first substrate 4, the first ion-selective electrode 6, the second substrate 3, the second ion-selective electrode 7 and the third substrate 1 are arranged in sequence from bottom to top; a first substrate 4 comprising first heating means for carrying and heating a first ion-selective electrode 6; a first ion-selective electrode 6 for carrying a first sample and collecting a first electric signal output by the first sample; a second substrate 3 for carrying a second ion-selective electrode 7; a third substrate 1 comprising second heating means for heating the second ion-selective electrode 7; a second ion selective electrode 7 for carrying a second sample and collecting a second electric signal output from the second sample; the first electric signal extraction connecting piece 5 is connected with the first ion selective electrode 6 and is used for extracting the first electric signal collected by the first ion selective electrode 6; the second electric signal extraction connecting piece 8 is connected with the second ion selective electrode 7 and is used for extracting the second electric signal collected by the second ion selective electrode 7; the electric signal acquisition circuit board 2 is respectively connected with the first electric signal lead-out connecting piece 5 and the second electric signal lead-out connecting piece 8 and is used for acquiring the first electric signal led out by the first electric signal lead-out connecting piece 5 and the second electric signal led out by the second electric signal lead-out connecting piece 8; and the processor 9 is connected with the electric signal acquisition circuit board 8 and is used for judging whether the first sample and the second sample are amplified or not according to the first electric signal and the second electric signal.
According to the dual-channel isothermal amplification device provided by the embodiment of the invention, the amplification result of the isothermal amplification technology is detected by adopting the electric signal of the sample collected by the ion selective electrode, so that the amplification result of the isothermal amplification technology can be accurately and rapidly judged; two samples can be detected simultaneously by setting two channels for isothermal amplification, and one sample can be set as a blank comparison sample so as to be convenient for comparison with the other sample, thereby reducing misjudgment of detection results; the ion selective electrode has simple manufacturing process and low cost, so that the detection cost of the amplification result of the isothermal amplification technology is low; and the ion selective electrode is small, so that the two-channel isothermal amplification device is small, and is convenient to carry and store.
In an alternative embodiment, the first heating device and the second heating device may be electric heating plates, electric heating wires, electric heating plates, or the like, and the invention is not limited thereto. Preferably, the embodiment of the present invention is described taking the first heating device and the second heating device as electric heating sheets as examples, where the first heating device includes the first heating sheet, and the second heating device includes the second heating sheet.
In an alternative embodiment, to reduce the trouble of excessive wiring, the first heating plate and the second heating plate may be in communication, i.e., the first heating plate and the second heating plate are the same heating plate.
In an alternative embodiment, the first ion-selective electrode may be any one of a hydrogen ion-selective electrode, a sodium ion-selective electrode, a potassium ion-selective electrode, a chloride ion-selective electrode, and a calcium ion-selective electrode; the second ion-selective electrode may be any one of a hydrogen ion-selective electrode, a sodium ion-selective electrode, a potassium ion-selective electrode, a chloride ion-selective electrode, and a calcium ion-selective electrode. In the embodiment of the present invention, the first ion-selective electrode and the second ion-selective electrode are exemplified as hydrogen ion-selective electrodes. The hydrogen ion selective electrode structure is shown in fig. 2, and comprises: a base layer 21, a measuring electrode layer 22, a reference electrode layer 27, an insulating layer 23, a solid electrolyte layer 24 of a measuring electrode, a solid electrolyte layer 28 of a reference electrode, a gas permeable layer 25 of a measuring electrode, a gas permeable layer 29 of a reference electrode, a bonding layer 20, a sample introduction layer 26 and a sealing film 30 which are laminated; wherein the measuring electrode layer 22 and the reference electrode layer 27 are positioned on the same layer, the solid electrolyte layer 24 of the measuring electrode and the solid electrolyte layer 28 of the reference electrode are positioned on the same layer, and the gas permeable layer 25 of the measuring electrode and the gas permeable layer 29 of the reference electrode are positioned on the same layer. The insulating layer 23 is provided with a first opening to bring the reference electrode layer 27 into contact with the solid electrolyte layer 28 of the reference electrode; and the insulating layer 23 is provided with a second opening so that the measuring electrode layer 22 is in contact with the solid electrolyte layer 24 of the measuring electrode; the gas permeable layer 29 of the reference electrode has a first region on a side of the solid electrolyte layer 28 of the reference electrode remote from the reference electrode layer 27, and a second region on the periphery of the first region; the second region is provided with an opening for communicating the solid electrolyte layer 28 of the reference electrode with the outside. The substrate layer 21 may be a PET plastic substrate or a carbon substrate. The measuring electrode layer 22 may be a carbon electrode, a gold electrode or other suitable conductive electrode, and the measuring electrode layer 22 may be formed by printing or vapor deposition. The material of the insulating layer 23 may be Polydimethylsiloxane (PDMS) or green oil. The material of the solid electrolyte layer 24 of the measuring electrode may be PEDOT, graphene or carbon nanotubes. The reference electrode layer 27 includes a conductive layer and a silver chloride layer which are stacked, and may be, for example, an Ag layer and an Agcl layer. The solid electrolyte layer 28 of the reference electrode is formed by mixing gelatin with a saturated chloride solution and drying. The gas permeable layer 25 of the measuring electrode is a measuring electrode film, which is used to cover the solid electrolyte layer 24 of the measuring electrode. The gas permeable layer 29 of the reference electrode is a reference electrode film for coating the solid electrolyte layer 28 of the reference electrode, the reference electrode film has a first area on one side of the solid electrolyte layer 28 of the reference electrode far away from the reference electrode layer 27, and a second area on the periphery of the first area, and an opening is formed on the second area to allow the solid electrolyte layer 28 of the reference electrode to communicate with the outside. The material of the sample introduction layer 26 is polyester fiber.
The ion selective electrode provided by the embodiment of the invention adopts the solid reference electrode, the solid reference electrolyte in the solid reference electrode has the property of converting ionic conduction into electronic conduction, and the solid reference electrode is arranged in the reference electrode to replace electrolyte solution, so that the solid reference electrode is obtained, the tolerance of the reference electrode to the environment is improved, the reference electrode is suitable for being soaked in a liquid detection environment, and the storage and the use are easy. The solid electrolyte layer of the reference electrode transfers electrons to the reference electrode layer, generating a reference potential, and the concentration of ions in the solid electrolyte layer of the reference electrode is fixed, so that a constant reference potential can be obtained. When the solid reference electrode is used for detecting a sample to be detected, the opening which exposes the solid electrolyte layer of the reference electrode is formed in the reference electrode film, so that water vapor in the sample enters the solid electrolyte layer of the reference electrode through the opening, the solid electrolyte in the sample is dissolved, the time required for generating liquid ions in the solid electrolyte layer is effectively shortened, and the time required for the solid reference electrode to reach a stable potential is greatly shortened. Meanwhile, the opening is arranged in the second area of the reference electrode film, so that the surface of the first solid electrolyte layer, which is in contact with the sample to be detected, is avoided, and ions in the detection environment can be prevented from entering the solid reference electrode through the opening while the water vapor transmission is accelerated, the interference of environmental factors is reduced, and the stability of the solid reference electrode is maintained. The time required for the solid reference electrode to reach the stable potential is short, so that the ion selective electrode can realize rapid detection of target parameters; on the other hand, the solid reference electrode has small potential fluctuation and high potential stability along with time, so that the ion selective electrode has high detection stability on the detection of target parameters.
The embodiment of the invention also provides a two-channel isothermal amplification method, which is applied to the two-channel isothermal amplification device in any embodiment, and comprises the following steps: placing the first sample and the second sample on the first ion-selective electrode and the second ion-selective electrode, respectively; heating the first ion-selective electrode and the second ion-selective electrode by a first heating device and a second heating device, respectively; collecting a first electric signal and a second point signal output by a first sample and a second sample respectively by using a first ion selective electrode and a second ion selective electrode; and judging whether the first sample and the second sample are amplified or not according to the first electric signal and the second electric signal by the processor.
Specifically, the first sample can be a blank reference sample, the second sample can be a sample to be detected containing a sample to be detected, the first sample and the second sample are respectively arranged on sample injection layers of a first ion selective electrode and a second ion selective electrode in a self-driven sample injection mode, sealing films of the first ion selective electrode and the second ion selective electrode are sealed, the first ion selective electrode and the second ion selective electrode are respectively arranged on a first substrate and a second substrate, a power supply of a first heating device and a power supply of a second heating device are connected, so that the first heating device and the second heating device respectively heat the first ion selective electrode and the second ion selective electrode, and meanwhile, the first ion selective electrode and the second ion selective electrode are used for respectively collecting a first electric signal and a second point signal output by the first sample and the second sample, the first electric signal and the second electric signal are respectively transmitted to the electric signal collecting circuit board through the first electric signal leading-out connecting piece and the second electric signal leading-out connecting piece, the electric signal collecting circuit board transmits the first electric signal and the second electric signal to the processor, the processor processes the first electric signal and the second electric signal and judges whether the first sample and the second sample are amplified or not, for example, if the first electric signal has a trend of gradually becoming smaller, the first sample is not amplified, and the second electric signal has a trend of gradually becoming larger, which is an indication that the second sample is amplified.
It will be appreciated by those skilled in the art that implementing all or part of the above-described embodiment method may be implemented by a computer program to instruct related hardware, where the program may be stored in a computer readable storage medium, and the program may include the above-described embodiment method when executed. Wherein the storage medium may be a magnetic Disk, an optical Disk, a Read-Only Memory (ROM), a random access Memory (RandomAccessMemory, RAM), a Flash Memory (Flash Memory), a Hard Disk (HDD), a Solid State Drive (SSD), or the like; the storage medium may also comprise a combination of memories of the kind described above.
Although embodiments of the present invention have been described in connection with the accompanying drawings, various modifications and variations may be made by those skilled in the art without departing from the spirit and scope of the invention, and such modifications and variations are within the scope of the invention as defined by the appended claims.
Claims (9)
1. A two-channel isothermal amplification device, comprising:
the first substrate, the first ion selective electrode, the second substrate, the second ion selective electrode and the third substrate are sequentially arranged from bottom to top;
the first substrate comprises a first heating device for bearing and heating the first ion selective electrode;
the first ion selective electrode is used for bearing a first sample and collecting a first electric signal output by the first sample;
the second substrate is used for bearing the second ion selective electrode;
the third substrate comprises a second heating device for heating the second ion-selective electrode;
the second ion selective electrode is used for bearing a second sample and collecting a second electric signal output by the second sample;
wherein the first ion-selective electrode and/or the second ion-selective electrode comprises:
the device comprises a basal layer, a measuring electrode layer, a reference electrode layer, an insulating layer, a solid electrolyte layer of the measuring electrode, a solid electrolyte layer of the reference electrode, a gas permeable layer of the measuring electrode, a gas permeable layer of the reference electrode, a bonding layer, a sample injection layer and a sealing film which are arranged in a laminated manner;
the measuring electrode layer and the reference electrode layer are positioned on the same layer, the solid electrolyte layer of the measuring electrode and the solid electrolyte layer of the reference electrode are positioned on the same layer, and the ventilation layer of the measuring electrode and the ventilation layer of the reference electrode are positioned on the same layer; the gas permeable layer of the reference electrode has a first region on a side of the solid electrolyte layer of the reference electrode remote from the reference electrode layer, and a second region at the periphery of the first region; an opening for communicating the solid electrolyte layer of the reference electrode with the outside is formed in the second region;
the first electric signal extraction connecting piece is connected with the first ion selective electrode and is used for extracting the first electric signal acquired by the first ion selective electrode;
the second electric signal extraction connecting piece is connected with the second ion selective electrode and is used for extracting the second electric signal acquired by the second ion selective electrode;
the electric signal acquisition circuit board is respectively connected with the first electric signal lead-out connecting piece and the second electric signal lead-out connecting piece and is used for acquiring the first electric signal led out by the first electric signal lead-out connecting piece and the second electric signal led out by the second electric signal lead-out connecting piece;
and the processor is connected with the electric signal acquisition circuit board and used for judging whether the first sample and the second sample are amplified or not according to the first electric signal and the second electric signal.
2. The two-channel isothermal amplification device according to claim 1, wherein,
the first heating device comprises a first heating plate, and the second heating device comprises a second heating plate.
3. The two-channel isothermal amplification device according to claim 2, wherein,
the first heating plate and the second heating plate are communicated.
4. A two-channel isothermal amplification device according to any of claims 1 to 3, wherein,
the first ion selective electrode is any one of a hydrogen ion selective electrode, a sodium ion selective electrode, a potassium ion selective electrode, a chloride ion selective electrode and a calcium ion selective electrode;
the second ion selective electrode is any one of a hydrogen ion selective electrode, a sodium ion selective electrode, a potassium ion selective electrode, a chloride ion selective electrode and a calcium ion selective electrode.
5. The two-channel isothermal amplification device according to claim 4, wherein,
a first opening is formed in the insulating layer, so that the reference electrode layer is in contact with the solid electrolyte layer of the reference electrode; and is also provided with
The insulating layer is provided with a second opening to allow the measuring electrode layer to be in contact with the solid electrolyte layer of the measuring electrode.
6. The two-channel isothermal amplification device according to claim 5, wherein,
the sample injection layer is made of polyester fiber.
7. The two-channel isothermal amplification device according to claim 6, wherein,
the solid electrolyte layer of the reference electrode is formed by mixing gelatin with saturated chloride ion solution and drying.
8. The two-channel isothermal amplification device according to claim 7, wherein,
the reference electrode layer comprises a conductive layer and a silver chloride layer which are stacked.
9. A two-channel isothermal amplification method applied to the two-channel isothermal amplification device according to any one of claims 1 to 8, comprising:
placing the first sample and the second sample on the first ion-selective electrode and the second ion-selective electrode, respectively;
heating the first ion-selective electrode and the second ion-selective electrode by a first heating device and a second heating device, respectively;
collecting a first electric signal and a second electric signal output by the first sample and the second sample respectively by using a first ion selective electrode and a second ion selective electrode;
determining, by the processor, whether amplification of the first and second samples occurs based on the first and second electrical signals.
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| CN109563462A (en) * | 2016-01-08 | 2019-04-02 | 先进诊疗公司 | Detect the fully-integrated handheld device of specific nucleic acid sequence |
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| CN107002005B (en) * | 2014-09-02 | 2021-05-11 | 东芝医疗***株式会社 | Nucleic acid detecting cassette |
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| CN101871912A (en) * | 2010-06-07 | 2010-10-27 | 浙江大学 | Full-solid potassium ion sensor and preparation method thereof |
| CN103645229A (en) * | 2013-12-12 | 2014-03-19 | 复旦大学 | Array type multi-electrochemical isothermal amplification chip for detecting bacteria and preparation method thereof |
| CN109563462A (en) * | 2016-01-08 | 2019-04-02 | 先进诊疗公司 | Detect the fully-integrated handheld device of specific nucleic acid sequence |
| JP2018130122A (en) * | 2018-05-22 | 2018-08-23 | 株式会社東芝 | Nucleic acid detection method and assay kit |
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