CN202066830U - Screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides - Google Patents
Screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides Download PDFInfo
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- CN202066830U CN202066830U CN2011200231703U CN201120023170U CN202066830U CN 202066830 U CN202066830 U CN 202066830U CN 2011200231703 U CN2011200231703 U CN 2011200231703U CN 201120023170 U CN201120023170 U CN 201120023170U CN 202066830 U CN202066830 U CN 202066830U
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- printing electrode
- enzyme
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides, which belongs to the technical field of environmental monitoring. The technical scheme for manufacturing the sensor includes that an electrode substrate is washed and disposed in a drying box to be dried, silver paste is printed on the substrate and then placed in the drying box to be dried, carbon paste is printed on the electrode substrate and then arranged in the drying box to be dried, and insulating paste is printed on the electrode substrate and then disposed in the drying box to be dried so that a screen printing electrode is formed and placed in a refrigerator at 4 DEG C for drying and storing. 20mu L enzyme liquor with concentration of 0.05U/mu L is applied on the screen printing electrode, the screen printing electrode is air-dried naturally, and acetyl chlorine enzyme is adsorbed and fixed physically. The screen printing electrode enzyme sensor can quickly, sensitively and effectively detect the organic phosphorous pesticides at low price.
Description
Technical field
The utility model belongs to the environmental monitoring technology field, is specifically related to a kind of screen printing electrode enzyme sensor of fast detecting organophosphorus pesticide.
Background technology
In recent years, the detection of organophosphorus pesticide was the direction that numerous environmental analysis workers make great efforts all the time in underground water, seawater or the waste water, and the organophosphorus pesticide in the soil also is to carry out test analysis again through after extracting liquid phase mostly.At present, often after, use gas chromatography, gas chromatography mass spectrometry, high performance liquid chromatography etc. for the detection of organophosphorus pesticide again through complicated pre-treatment.Organophosphorus pesticide is highly sensitive, degree of accuracy good though these methods are measured, but in this alanysis method system with determinand separation and Extraction and to remove the pre-treatment process that coexistent impurity disturbs very complicated from sample, need through complicated sample pre-treatments such as extracting, purify, concentrate and derive, require high to laboratory and instrumentation degree, and during operating cost, the analysis cost costliness is unfavorable for promoting the use of, and is difficult to adapt to great amount of samples and the on-the-spot requirement that detects.Therefore, seek a kind of quick, sensitive and cheap effectively detection side's religious services or rituals must go.
The utility model provides a kind of screen printing electrode enzyme sensor of fast detecting organophosphorus pesticide, the organophosphorus pesticide in the energy fast detecting water body.
Summary of the invention
The technical scheme that the utility model adopted is: the cleaning electrode substrate places drying box dry; Printed silver slurry on substrate is placed in the drying box dry; Carbon slurry in the printing is placed in the drying box dry; Insulation slurry in the printing is placed on dry screen printing electrode in the drying box; Electrode is positioned over kept dry in 4 ℃ of refrigerators.With 20 μ L concentration is that the enzyme solutions of 0.05U/ μ L spreads upon on the silk screen electrode, and natural air drying is by the fixing enzyme acetylcholine of physisorption.
The beneficial effects of the utility model are quick, sensitive and effectively cheap.
Description of drawings
Accompanying drawing 1 is the vertical view of enzyme sensor.In the accompanying drawing 1,1. being conductive carbon paste, 2. is conductive silver paste, for the insulation slurry, 4. is substrate 3., 5. is to electrode, 6. is the electrode perform region, 7. is working electrode, 8. is contrast electrode.
Accompanying drawing 2 is response current change curves.
Accompanying drawing 3 is different terbufos concentration-response electric current change curves.
Embodiment
Further specify the present invention below in conjunction with example.
Material:
(1) electrode base board.
(2) silver slurry, insulation slurry, carbon slurry.
(3) enzyme acetylcholine.
(4) potassium ferricyanide solution.
(5) phosphate buffered solution.
(6) chlorinated thio acetylcholine solution.
(7) organophosphorus pesticide terbufos solution.
Embodiment
The cleaning electrode substrate places drying box dry; Printed silver slurry on substrate places drying box dry then; Carbon slurry in the printing places drying box dry then; Insulation slurry in the printing, place then drying box dry screen printing electrode; Electrode is positioned over kept dry in 4 ℃ of refrigerators.With 20 μ L concentration is that the enzyme solutions of 0.05U/ μ L spreads upon on the screen printing electrode, and natural air drying is by the fixing enzyme acetylcholine of physisorption.With 30 μ L concentration is that 0.05U/ μ L acetylcholine enzyme solutions spreads upon on the electrode, natural air drying, and the method by physisorption makes it fixing.Drip the solution or the blank phosphate buffered solution of the certain density organophosphorus pesticide terbufos of 20 μ L in the electrode perform region, cover the fixing regional 10min of enzyme.Be that potassium ferricyanide calibration solution and the 50 μ L concentration of 0.2mmol/L are 5mmol/L chlorinated thio acetylcholine substrate solution in electrode perform region Dropwise 50 μ L concentration successively, galvanostatic method detects size of current behind the 15min.
Claims (1)
1. the screen printing electrode enzyme sensor of a fast detecting organophosphorus pesticide, it is characterized in that, the silk screen electrode of this enzyme sensor is printed silver slurry, carbon slurry and the formation of insulation slurry successively on electrode base board, and the absorption fixed concentration is that the amount of the enzyme acetylcholine of 0.05U/ μ L is 20 μ L on the silk screen electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200231703U CN202066830U (en) | 2011-01-25 | 2011-01-25 | Screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200231703U CN202066830U (en) | 2011-01-25 | 2011-01-25 | Screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202066830U true CN202066830U (en) | 2011-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011200231703U Expired - Fee Related CN202066830U (en) | 2011-01-25 | 2011-01-25 | Screen printing electrode enzyme sensor capable of quickly detecting organic phosphorous pesticides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202066830U (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018305A (en) * | 2012-12-11 | 2013-04-03 | 江苏大学 | Portable enzyme sensor based on carbon nanometer tube |
| CN103123333A (en) * | 2012-12-31 | 2013-05-29 | 北京师范大学 | Method for rapidly detecting lead based on three-electrode sensor and differential pulse voltammetry |
| CN103149265A (en) * | 2012-12-31 | 2013-06-12 | 北京师范大学 | Quick copper-testing method based on linear sweep voltammetry and three-electrode sensor |
| CN103913502A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on square-wave stripping voltammetry and three-electrode sensor |
| CN104034798A (en) * | 2013-03-07 | 2014-09-10 | 浙江工商大学 | Cell suspension concentration detection system and its detection method |
| CN104569093A (en) * | 2015-01-23 | 2015-04-29 | 三诺生物传感股份有限公司 | Carbon paste for electrochemical detection test strip, preparation method of carbon paste, electrochemical detection test strip and preparation method of electrochemical detection test strip |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| CN108398477A (en) * | 2018-01-22 | 2018-08-14 | 昆明理工大学 | A kind of active electrochemical method of measurement phosphoric acid enol pyruvic acid carboxylase |
-
2011
- 2011-01-25 CN CN2011200231703U patent/CN202066830U/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018305A (en) * | 2012-12-11 | 2013-04-03 | 江苏大学 | Portable enzyme sensor based on carbon nanometer tube |
| CN103123333A (en) * | 2012-12-31 | 2013-05-29 | 北京师范大学 | Method for rapidly detecting lead based on three-electrode sensor and differential pulse voltammetry |
| CN103149265A (en) * | 2012-12-31 | 2013-06-12 | 北京师范大学 | Quick copper-testing method based on linear sweep voltammetry and three-electrode sensor |
| CN103913502A (en) * | 2012-12-31 | 2014-07-09 | 北京师范大学 | Copper rapid determination method based on square-wave stripping voltammetry and three-electrode sensor |
| CN103149265B (en) * | 2012-12-31 | 2016-03-02 | 北京师范大学 | Based on the method for linear sweep voltammetry and three electrode sensor Fast Measurement copper |
| CN103123333B (en) * | 2012-12-31 | 2016-03-02 | 北京师范大学 | Based on the method for three electrode sensors and Differential Pulse Voltammetry Fast Measurement lead |
| CN103913502B (en) * | 2012-12-31 | 2017-12-26 | 北京师范大学 | The method for quickly determining copper based on square wave stripping voltammetry and three electrode sensors |
| CN104034798A (en) * | 2013-03-07 | 2014-09-10 | 浙江工商大学 | Cell suspension concentration detection system and its detection method |
| CN104569093A (en) * | 2015-01-23 | 2015-04-29 | 三诺生物传感股份有限公司 | Carbon paste for electrochemical detection test strip, preparation method of carbon paste, electrochemical detection test strip and preparation method of electrochemical detection test strip |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| CN108398477A (en) * | 2018-01-22 | 2018-08-14 | 昆明理工大学 | A kind of active electrochemical method of measurement phosphoric acid enol pyruvic acid carboxylase |
| CN108398477B (en) * | 2018-01-22 | 2020-08-25 | 昆明理工大学 | Electrochemical method for determining activity of phosphoenolpyruvate carboxylase |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111207 Termination date: 20130125 |