CN112129604A - Method and equipment for preparing hydrolyzed amino acid - Google Patents
Method and equipment for preparing hydrolyzed amino acid Download PDFInfo
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- CN112129604A CN112129604A CN202011005210.1A CN202011005210A CN112129604A CN 112129604 A CN112129604 A CN 112129604A CN 202011005210 A CN202011005210 A CN 202011005210A CN 112129604 A CN112129604 A CN 112129604A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
The invention discloses a method and equipment for preparing hydrolyzed amino acid, which relate to the technical field of amino acid preparation, have the advantage of being capable of detecting whether oxygen in a hydrolysis bottle is emptied, and have the technical scheme key points that: s1: weighing a sample: s2: putting the weighed sample into a hydrolysis bottle, adding hydrochloric acid into the hydrolysis bottle, and screwing a sealing cover; s3: emptying oxygen in the hydrolysis bottle: the sealing cover is provided with an air inlet pipe for discharging nitrogen, an air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, the hydrolysis bottle is firstly filled with the nitrogen, the sealing cover is provided with an exhaust pipe for discharging oxygen, the length of the exhaust pipe in the hydrolysis bottle is adjustable, so that the exhaust pipe is close to the liquid level in the hydrolysis bottle, the exhaust pipe is provided with an oxygen concentration tester, whether the hydrolysis bottle contains the oxygen is judged according to the reading on the oxygen concentration tester, and the air inlet pipe and the exhaust pipe are both provided with stop valves; s4: hydrolyzing the amino acid; s5: opening the tube to take the liquid, filtering and washing, evaporating to dryness and deacidifying, buffering and dissolving, and taking out the supernatant.
Description
Technical Field
The invention relates to the technical field of amino acid preparation, in particular to a method and equipment for preparing hydrolyzed amino acid.
Background
An amino acid is a compound in which a hydrogen atom on a carbon atom of a carboxylic acid is substituted with an amino group, and the amino acid molecule contains both amino and carboxyl functional groups. Amino acids, like hydroxy acids, can be classified as α -, β -, γ -, w-amino acids according to the position of the amino group attached to the carbon chain, but the amino acids obtained after proteolysis are all α -amino acids, and only twenty, which are the basic units constituting proteins.
Chinese patent with publication number CN110668907A discloses a method for preparing hydrolyzed amino acid, which comprises the following steps: s1: weighing a sample: accurately weighing a hydrolysis sample according to the content of amino acid in a protein sample, so that the content of the amino acid taken out is 10-20 mg; s2: putting the weighed sample into a hydrolysis bottle, adding hydrochloric acid into the hydrolysis bottle, and screwing a sealing cover; s3: sealing the hydrolysis bottle: firstly, filling nitrogen into a hydrolysis bottle, then vacuumizing the hydrolysis bottle, and repeating the steps for many times to ensure that the hydrolysis bottle is completely free of oxygen; s4: hydrolyzing amino acid: placing the sealed hydrolysis bottle in a constant-temperature drying box at 110 ℃, standing for 24-30 hours, fully reacting the sample in the hydrolysis bottle with hydrochloric acid, and breaking peptide bonds of protein in the sample by the hydrochloric acid to form amino acid; s5: opening the tube to take liquid: taking the hydrolysis bottle out of the drying box, placing the hydrolysis bottle in a shade place, naturally cooling the hydrolysis bottle, and unscrewing a sealing cover of the hydrolysis bottle to pour out a hydrolyzed sample after the hydrolysis box is cooled; s6, filtering and washing: filtering the substances in the hydrolysis bottle, pouring the substances into a volumetric flask, and removing the residual impurities after the hydrolysis of the sample; washing the hydrolysis bottle with ionic water, filtering the washed solution with filter paper, and pouring the filtered solution into a volumetric flask; s7: evaporating to dryness and deacidifying: taking out the materials in the volumetric flask, placing the materials on a vacuum deacidification instrument for deacidification, and removing the materials until the materials are dried, wherein a little solid or trace stains are left at the bottom of the materials; s8: buffering and dissolving: adding a sodium citrate buffer solution with the pH value of 2.2 into the deacidified sample, and dissolving the sample; s9: taking out the supernatant: and taking out the supernatant after the sample is diluted by the buffer solution for storage.
But at S3: the sealed hydrolysis bottle needs to be vacuumized after being filled with nitrogen, and needs to be repeatedly vacuumized, a detection device does not detect whether oxygen in the hydrolysis bottle is emptied, and the steps are complicated.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a method for preparing hydrolyzed amino acid, which has the advantage of being capable of detecting whether oxygen in a hydrolysis bottle is emptied or not.
In order to achieve the purpose, the invention provides the following technical scheme: a method for preparing hydrolyzed amino acid comprises the following steps,
s1: weighing a sample: weighing a hydrolysis sample containing amino acid;
s2: putting the weighed sample into a hydrolysis bottle, adding hydrochloric acid into the hydrolysis bottle, and screwing a sealing cover;
s3: emptying oxygen in the hydrolysis bottle: the sealing cover is provided with an air inlet pipe for discharging nitrogen, an air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, the hydrolysis bottle is firstly filled with the nitrogen, the sealing cover is provided with an exhaust pipe for discharging oxygen, the length of the exhaust pipe in the hydrolysis bottle is adjustable, so that the exhaust pipe is close to the liquid level in the hydrolysis bottle, the exhaust pipe is provided with an oxygen concentration tester, whether the hydrolysis bottle contains the oxygen is judged according to the reading on the oxygen concentration tester, and the air inlet pipe and the exhaust pipe are both provided with stop valves;
s4: hydrolyzing amino acid: placing the sealed hydrolysis bottle in a constant-temperature drying box at 110 ℃, placing for 30 hours, fully reacting the sample in the hydrolysis bottle with hydrochloric acid, and breaking peptide bonds of protein in the sample by the hydrochloric acid to form amino acid;
s5: opening the tube to take liquid, filtering and washing, evaporating to dryness and deacidifying, buffering and dissolving, and taking out supernatant fluid: and taking out the supernatant after the sample is diluted by the buffer solution for storage.
By adopting the technical scheme, the weighed sample is put into a hydrolysis bottle, hydrochloric acid is added into the hydrolysis bottle, after the sealing cover is screwed, nitrogen is introduced into the hydrolysis bottle through the air inlet pipe, because the nitrogen is lighter than the oxygen, the air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, so that the nitrogen can be conveniently and gradually filled in the space above the hydrolysis bottle, and the oxygen is extruded into the exhaust pipe, the exhaust pipe is positioned at a position close to the liquid level after the length of the exhaust pipe is adjusted, so that the oxygen in the hydrolysis bottle can be conveniently discharged out of the hydrolysis bottle, the oxygen concentration tester is convenient for a user to visually read the content of the oxygen in the exhaust pipe, thereby ensuring the discharge of the oxygen in the hydrolysis bottle, further detecting whether the oxygen in the hydrolysis bottle is emptied or not, facilitating the subsequent processes, when blast pipe and intake pipe stop work, intake pipe and blast pipe are closed to the stop valve, are convenient for hydrolyze the reaction in the bottle.
The invention also aims to provide a hydrolysis bottle applied to the preparation method of the hydrolyzed amino acid, the hydrolysis bottle is a long-neck glass test tube, the mouth of the hydrolysis bottle is in threaded connection with a sealing cover, an air inlet pipe for discharging nitrogen is arranged on the sealing cover, the air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, an exhaust pipe for discharging oxygen is arranged on the sealing cover, a telescopic pipe made of plastic cloth is arranged at the pipe orifice of one end of the exhaust pipe, which is positioned in the hydrolysis bottle, a counterweight ring is arranged at one end of the telescopic pipe, which is far away from the exhaust pipe, an adjusting part for adjusting the height position of the counterweight ring is arranged at one end of the exhaust pipe, which is positioned outside the hydrolysis.
Preferably: the adjusting part comprises a pull rope arranged on the balance weight ring, the inner wall of the exhaust pipe is rotatably connected with a rotating rod, one end, far away from the balance weight ring, of the pull rope is fixed on the rotating rod and wound on the rotating rod, a driving groove is formed in the outer wall, corresponding to the rotating rod, of the outer wall of the exhaust pipe, the rotating rod is an ironwork product, and a magnet corresponding to the rotating rod is rotatably connected in the driving groove.
Preferably: one end of the counterweight ring, which is far away from the telescopic pipe, is provided with a conical air suction cover.
Preferably: after the air inlet pipe and the exhaust pipe stop working, the stop valves on the air inlet pipe and the exhaust pipe stop working, and the ends, far away from the sealing covers, of the air inlet pipe and the exhaust pipe are provided with sealing parts for sealing the air inlet pipe and the exhaust pipe.
Preferably: the sealing part comprises a sealing plate which is arranged on the inner wall of one end, far away from the sealing cover, of the air inlet pipe or the exhaust pipe, a tapered hole is formed in the sealing plate, a tapered plug for sealing the tapered hole is arranged on the sealing plate, the sealing plate is located at one end, far away from the sealing cover, of the stop valve, and a driving piece for driving the tapered plug to open or seal the tapered hole is arranged on the outer wall of the air inlet pipe or the exhaust pipe.
Preferably: the driving piece is including seting up on intake pipe or blast pipe and being located the connecting hole that sealed lid one end was kept away from to the closing plate, the connecting hole is along intake pipe or blast pipe length direction distribution just it is connected with the driving lever to slide in the connecting hole, intake pipe or blast pipe all rotate the outer wall that has the connecting hole and are connected with the closed tube, when the closed tube rotates, the spiral groove that supplies the driving lever to remove is seted up to the closed tube inner wall, the one end that the closed tube was kept away from to the driving lever is kept away from bell mouth one end fixed connection with the toper stopper, when rotating the closed tube and making the driving lever reciprocate to required position in the connecting hole, be equipped with the.
Preferably: the locating part includes the check lock of threaded connection at the closed tube outer wall, the check lock passes the closed tube and contradicts with intake pipe or blast pipe outer wall.
Preferably: and a rubber pad is arranged at one end of the locking rod, which is abutted against the outer wall of the air inlet pipe or the exhaust pipe.
Preferably: when the sealing cover is screwed into the hydrolysis bottle, the upper end of the sealing cover is provided with an annular sealing plate which is abutted against the mouth of the hydrolysis bottle.
In conclusion, the invention has the following beneficial effects: put into the bottle of hydrolysising with the good sample of title, add hydrochloric acid in the bottle of hydrolysising, sealed lid is twisted the back, let in nitrogen gas in to the bottle of hydrolysising through the intake pipe, because nitrogen gas is lighter than oxygen, consequently flush the gas outlet of intake pipe and sealed lid towards one side in the bottle of hydrolysising, be convenient for nitrogen gas is gradually full of bottle top space of hydrolysising, and extrude oxygen in to the blast pipe, be located the position that is close to the liquid level behind the blast pipe regulation length, be convenient for with the bottle of hydrolysising interior oxygen discharge hydrolysis bottle, oxygen concentration tester person's audio-visual reading of this moment is interior oxygen content in the blast pipe, and then ensure the discharge of the interior oxygen of bottle of hydrolysising, and then detect whether the oxygen in the bottle of hydrolysising is evacuated, be convenient for going on of follow-up process, when blast pipe and intake pipe stop work, intake pipe and blast.
Drawings
FIG. 1 is a schematic structural view of example 2;
fig. 2 is an enlarged schematic view of a portion a of fig. 1.
In the figure: 1. a hydrolysis bottle; 11. a sealing cover; 12. an air inlet pipe; 13. an exhaust pipe; 131. a telescopic pipe; 132. a counterweight ring; 133. a stop valve; 134. pulling a rope; 135. a rotating rod; 136. a drive slot; 137. a magnet; 14. an air intake cover; 15. a closing plate; 151. a tapered hole; 152. a conical plug; 16. connecting holes; 161. a deflector rod; 162. closing the tube; 163. a spiral groove; 164. a locking lever; 165. a rubber pad; 17. and (7) sealing the plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a method for preparing hydrolyzed amino acid comprises the following steps,
s1: weighing a sample: weighing a hydrolysis sample containing amino acid;
s2: putting the weighed sample into a hydrolysis bottle, adding hydrochloric acid into the hydrolysis bottle, and screwing a sealing cover;
s3: emptying oxygen in the hydrolysis bottle: the sealing cover is provided with an air inlet pipe for discharging nitrogen, an air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, the hydrolysis bottle is firstly filled with the nitrogen, the sealing cover is provided with an exhaust pipe for discharging oxygen, the length of the exhaust pipe in the hydrolysis bottle is adjustable, so that the exhaust pipe is close to the liquid level in the hydrolysis bottle, the exhaust pipe is provided with an oxygen concentration tester, whether the hydrolysis bottle contains the oxygen is judged according to the reading on the oxygen concentration tester, and the air inlet pipe and the exhaust pipe are both provided with stop valves;
s4: hydrolyzing amino acid: placing the sealed hydrolysis bottle in a constant-temperature drying box at 110 ℃, placing for 30 hours, fully reacting the sample in the hydrolysis bottle with hydrochloric acid, and breaking peptide bonds of protein in the sample by the hydrochloric acid to form amino acid;
s5: opening the tube to take liquid, filtering and washing, evaporating to dryness and deacidifying, buffering and dissolving, and taking out supernatant fluid: taking out the supernatant after the sample is diluted by the buffer solution for storage, opening the tube to take out the solution, filtering and washing, evaporating to dryness and deacidifying, buffering and dissolving, and taking out the supernatant, and the consistency of the preparation method of the hydrolyzed amino acid disclosed in the Chinese patent with the publication number of CN110668907A is not repeated here.
Put into the bottle of hydrolysising with the good sample of title, add hydrochloric acid in the bottle of hydrolysising, sealed lid is twisted the back, let in nitrogen gas in to the bottle of hydrolysising through the intake pipe, because nitrogen gas is lighter than oxygen, consequently flush the gas outlet of intake pipe and sealed lid towards one side in the bottle of hydrolysising, be convenient for nitrogen gas is gradually full of bottle top space of hydrolysising, and extrude oxygen in to the blast pipe, be located the position that is close to the liquid level behind the blast pipe regulation length, be convenient for with the bottle of hydrolysising interior oxygen discharge hydrolysis bottle, oxygen concentration tester person's audio-visual reading of this moment is interior oxygen content in the blast pipe, and then ensure the discharge of the interior oxygen of bottle of hydrolysising, and then detect whether the oxygen in the bottle of hydrolysising is evacuated, be convenient for going on of follow-up process, when blast pipe and intake pipe stop work, intake pipe and blast.
Example 2: a hydrolysis bottle applied to the embodiment 1 is shown in figures 1 and 2, the hydrolysis bottle 1 is a long-neck glass test tube, the mouth of the hydrolysis bottle 1 is in threaded connection with the sealing cover 11, when the sealing cover 11 is screwed into the hydrolysis bottle 1, the upper end of the sealing cover 11 is provided with an annular sealing plate 17 which is abutted against the mouth of the hydrolysis bottle 1, and at the moment, the sealing plate 17 can be an elastic plate, so that the gap between the sealing cover 11 and the hydrolysis bottle 1 is reduced.
As shown in fig. 1 and fig. 2, an air inlet pipe 12 for discharging nitrogen is arranged on the sealing cover 11, an air outlet of the air inlet pipe 12 is flush with one side of the sealing cover 11 facing the inside of the hydrolysis bottle 1, an exhaust pipe 13 for discharging oxygen is arranged on the sealing cover 11, an orifice at one end of the exhaust pipe 13, which is located inside the hydrolysis bottle 1, is provided with a telescopic pipe 131 made of plastic cloth, the pipe diameter of the telescopic pipe 131 is consistent with that of the exhaust pipe 13, one end of the telescopic pipe 131, which is far away from the exhaust pipe 13, is provided with a counterweight ring 132, an adjusting part for adjusting the height position of the counterweight ring 132 is arranged at one end of the exhaust pipe 13. The adjusting part is convenient for adjust the height of counter weight ring 132, and then be convenient for adjust the length of flexible pipe 131, flexible pipe 131 is made by the plastic cloth this moment, be convenient for warp, the setting of counter weight ring 132 is convenient for stabilize the position that flexible pipe 131 kept away from blast pipe 13 one end, the adjusting part has adjusted the distance between blast pipe 13 and the interior liquid level of hydrolysis bottle 1 this moment, be convenient for blast pipe 13 to discharge the oxygen that is located hydrolysis bottle 1 and is close to the liquid level, and the adjusting part can adjust the position of counter weight ring 132 according to the height of liquid level at will, when intake pipe 12 fills nitrogen gas into hydrolysis bottle 1 in, nitrogen gas is because lighter than oxygen, be located the top of oxygen and extrude oxygen to the liquid level direction, be convenient for oxygen to let in.
As shown in fig. 1 and 2, the adjusting member includes a pulling rope 134 disposed on the counterweight ring 132, the inner wall of the exhaust pipe 13 is rotatably connected with a rotating rod 135, one end of the pulling rope 134, which is far away from the counterweight ring 132, is fixed on the rotating rod 135 and is wound on the rotating rod 135, the outer wall of the exhaust pipe 13 is provided with a driving groove 136 on the outer wall corresponding to the rotating rod 135, the rotating rod 135 is made of iron, a magnet 137 corresponding to the rotating rod 135 is rotatably connected in the driving groove 136, at this time, although the magnet 137 and the rotating rod 135 are separated by a pipe wall of the exhaust pipe 13, the magnet 137 can still attract the rotating rod 135, so that a user can drive the rotating rod 135 to rotate when rotating the magnet 137, and further the pulling rope 134 is wound or wound around the rotating rod 135, and further, the counterweight ring 132 is conveniently pulled upwards or the counterweight.
As shown in fig. 1 and 2, the end of the counterweight ring 132 remote from the telescopic tube 131 is provided with a conical suction hood 14. The provision of the suction cover 14 facilitates an increase in the area of oxygen entering the exhaust pipe 13.
As shown in fig. 1 and fig. 2, after the air inlet pipe 12 and the air outlet pipe 13 stop working, the stop valves 133 on the air inlet pipe 12 and the air outlet pipe 13 stop working, and the ends of the air inlet pipe 12 and the air outlet pipe 13 far away from the sealing cover 11 are provided with sealing parts for sealing the air inlet pipe 12 and the air outlet pipe 13, and the arrangement of the sealing parts reduces the influence of air entering the hydrolysis bottle 1 through the air inlet pipe 12 or the air outlet pipe 13 on the subsequent processes.
As shown in fig. 1 and fig. 2, the closing member includes a closing plate 15 disposed on an inner wall of one end of the intake pipe 12 or the exhaust pipe 13 away from the sealing cover 11, a tapered hole 151 is opened on the closing plate 15, a tapered plug 152 for closing the tapered hole 151 is disposed on the closing plate 15, the closing plate 15 is located at one end of the stop valve 133 away from the sealing cover 11, a driving member for driving the tapered plug 152 to open or close the tapered hole 151 is disposed on an outer wall of the intake pipe 12 or the exhaust pipe 13, and at this time, one end of the tapered hole 151 with a smaller taper is. When the driving member drives the conical plug 152 to close the conical hole 151, the opening of the air inlet pipe 12 or the air outlet pipe 13 is closed.
As shown in fig. 1 and fig. 2, the driving member includes a connecting hole 16 disposed on the air inlet pipe 12 or the air outlet pipe 13 and located at an end of the sealing plate 15 away from the sealing cover 11, the connecting hole 16 is distributed along a length direction of the air inlet pipe 12 or the air outlet pipe 13, and a shift lever 161 is slidably connected in the connecting hole 16, the air inlet pipe 12 or the air outlet pipe 13 is rotatably connected to a sealing pipe 162 on an outer wall having the connecting hole 16, when the sealing pipe 162 rotates, a spiral groove 163 for moving the shift lever 161 is disposed on an inner wall of the sealing pipe 162, an end of the shift lever 161 away from the sealing pipe 162 is fixedly connected to an end of the conical plug 152 away from the conical hole 151, and when the shift lever 161 reciprocally moves to a desired position in the connecting hole 16.
Referring to fig. 1 and 2, when it is required to close the tapered hole 151, a user rotates the closing tube 162, so that the shift lever 161 in the closing tube 162 moves along the spiral groove 163, at this time, the spiral groove 163 drives the shift lever 161 to reciprocate in the connection hole 16, when the closing tube 162 is rotated, the shift lever 161 drives the tapered plug 152 to move in the tapered hole 151, the tapered plug 152 closes the tapered hole 151, so as to reduce the flow of gas in the exhaust pipe 13 or the intake pipe 12, and the position of the closing tube 162 is limited by the limiting member, so that the position of the tapered plug 152 is fixed.
Referring to fig. 1 and 2, the stopper includes a locking rod 164 screwed to the outer wall of the closed pipe 162, and the locking rod 164 passes through the closed pipe 162 to abut against the outer wall of the intake pipe 12 or the exhaust pipe 13. When the position of the closed pipe 162 needs to be fixed, the locking rod 164 is rotated towards the outer wall of the air inlet pipe 12 or the air outlet pipe 13 until the locking rod abuts against the outer wall of the air inlet pipe 12 or the air outlet pipe 13, so that the rotation of the closed pipe 162 on the outer wall of the air inlet pipe 12 or the air outlet pipe 13 is limited.
As shown in fig. 1 and 2, a rubber pad 165 is disposed at one end of the locking rod 164 abutting against the outer wall of the intake pipe 12 or the exhaust pipe 13. The rubber pad 165 increases the friction between the locking lever 164 and the outer wall of the intake pipe 12 or the exhaust pipe 13, facilitating the fastening of the position of the closed pipe 162.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (10)
1. A method for preparing hydrolyzed amino acid is characterized in that: comprises the following steps of (a) carrying out,
s1: weighing a sample: weighing a hydrolysis sample containing amino acid;
s2: putting the weighed sample into a hydrolysis bottle, adding hydrochloric acid into the hydrolysis bottle, and screwing a sealing cover;
s3: emptying oxygen in the hydrolysis bottle: the sealing cover is provided with an air inlet pipe for discharging nitrogen, an air outlet of the air inlet pipe is flush with one side of the sealing cover facing the inside of the hydrolysis bottle, the hydrolysis bottle is firstly filled with the nitrogen, the sealing cover is provided with an exhaust pipe for discharging oxygen, the length of the exhaust pipe in the hydrolysis bottle is adjustable, so that the exhaust pipe is close to the liquid level in the hydrolysis bottle, the exhaust pipe is provided with an oxygen concentration tester, whether the hydrolysis bottle contains the oxygen is judged according to the reading on the oxygen concentration tester, and the air inlet pipe and the exhaust pipe are both provided with stop valves;
s4: hydrolyzing amino acid: placing the sealed hydrolysis bottle in a constant-temperature drying box at 110 ℃, placing for 30 hours, fully reacting the sample in the hydrolysis bottle with hydrochloric acid, and breaking peptide bonds of protein in the sample by the hydrochloric acid to form amino acid;
s5: opening the tube to take liquid, filtering and washing, evaporating to dryness and deacidifying, buffering and dissolving, and taking out supernatant fluid: and taking out the supernatant after the sample is diluted by the buffer solution for storage.
2. A hydrolysis flask to be used in the process for producing a hydrolyzed amino acid according to claim 1, wherein: hydrolysis bottle (1) is the long-neck glass test tube, hydrolysis bottle (1) bottleneck and sealed lid (11) threaded connection, be equipped with intake pipe (12) that emit into nitrogen gas on sealed lid (11), the gas outlet of intake pipe (12) flushes with sealed lid (11) towards one side in hydrolysis bottle (1), be equipped with exhaust pipe (13) of exhaust oxygen on sealed lid (11), the one end mouth of pipe that exhaust pipe (13) are located hydrolysis bottle (1) is equipped with flexible pipe (131) that the plastic cloth made, the one end that exhaust pipe (13) were kept away from in flexible pipe (131) is equipped with counter weight ring (132), the one end that exhaust pipe (13) are located hydrolysis bottle (1) is equipped with the regulating part that is used for adjusting counter weight ring (132) high position, all be equipped with stop valve (133) on intake pipe (12) and exhaust pipe (13).
3. The hydrolysis flask as set forth in claim 2, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: the regulating part is including setting up stay cord (134) on counter weight ring (132), blast pipe (13) inner wall rotates and is connected with bull stick (135), the one end that counter weight ring (132) were kept away from in stay cord (134) is fixed on bull stick (135) and is twined on bull stick (135), drive groove (136) have been seted up at the outer wall that corresponds bull stick (135) to blast pipe (13) outer wall, bull stick (135) are ironwork, drive groove (136) internal rotation is connected with magnet (137) corresponding with bull stick (135).
4. The hydrolysis flask as set forth in claim 1 or 2, which is used in a method for preparing a hydrolyzed amino acid, wherein: one end of the counterweight ring (132) far away from the telescopic pipe (131) is provided with a conical air suction cover (14).
5. The hydrolysis flask as set forth in claim 2, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: after the air inlet pipe (12) and the exhaust pipe (13) stop working, stop valves (133) on the air inlet pipe (12) and the exhaust pipe (13) stop working, and one ends, far away from the sealing cover (11), of the air inlet pipe (12) and the exhaust pipe (13) are provided with sealing parts for sealing the air inlet pipe (12) and the exhaust pipe (13).
6. The hydrolysis flask as set forth in claim 5, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: the closing member is including setting up closing plate (15) of keeping away from the one end inner wall of sealed lid (11) in intake pipe (12) or blast pipe (13), tapered hole (151) have been seted up on closing plate (15), be equipped with tapered plug (152) that seal tapered hole (151) on closing plate (15), closing plate (15) are located stop valve (133) and keep away from the one end of sealed lid (11), intake pipe (12) or blast pipe (13) outer wall are equipped with the driving piece that drive tapered plug (152) were opened or are sealed tapered hole (151).
7. The hydrolysis flask as set forth in claim 6, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: the driving piece comprises a connecting hole (16) which is arranged on the air inlet pipe (12) or the air outlet pipe (13) and is positioned at one end of the closing plate (15) far away from the sealing cover (11), the connecting holes (16) are distributed along the length direction of the air inlet pipe (12) or the air outlet pipe (13), the connecting holes (16) are connected with a shift lever (161) in a sliding way, the air inlet pipe (12) or the air outlet pipe (13) is rotatably connected with a closed pipe (162) on the outer wall with a connecting hole (16), when the closed pipe (162) rotates, the inner wall of the closed pipe (162) is provided with a spiral groove (163) for the shift lever (161) to move, one end of the deflector rod (161) far away from the closed pipe (162) is fixedly connected with one end of the conical plug (152) far away from the conical hole (151), when the closing pipe (162) is rotated to make the shift lever (161) move back and forth to the required position in the connecting hole (16), and a limiting piece for limiting the position of the closed pipe (162) is arranged on the closed pipe (162).
8. The hydrolysis flask as set forth in claim 7, wherein the hydrolysis flask is used in a method for preparing a hydrolyzed amino acid, and comprises: the locating part comprises a locking rod (164) which is in threaded connection with the outer wall of the closed pipe (162), and the locking rod (164) penetrates through the closed pipe (162) to be abutted against the outer wall of the air inlet pipe (12) or the air exhaust pipe (13).
9. The hydrolysis flask as set forth in claim 8, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: and a rubber pad (165) is arranged at one end of the locking rod (164) abutting against the outer wall of the air inlet pipe (12) or the exhaust pipe (13).
10. The hydrolysis flask as set forth in claim 2, wherein the hydrolysis flask is used in a method for producing a hydrolyzed amino acid, and comprises: when the sealing cover (11) is screwed into the hydrolysis bottle (1), the upper end of the sealing cover (11) is provided with an annular sealing plate (17) which is abutted against the mouth of the hydrolysis bottle (1).
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| CN202011005210.1A CN112129604A (en) | 2020-09-23 | 2020-09-23 | Method and equipment for preparing hydrolyzed amino acid |
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| CN202011005210.1A CN112129604A (en) | 2020-09-23 | 2020-09-23 | Method and equipment for preparing hydrolyzed amino acid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112816620A (en) * | 2020-12-31 | 2021-05-18 | 杭州谱育科技发展有限公司 | Gas concentration adjusting device and method |
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Application publication date: 20201225 |