CN112119973A - Method for increasing survival rate of bullfrog tadpoles in early stage - Google Patents
Method for increasing survival rate of bullfrog tadpoles in early stage Download PDFInfo
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- CN112119973A CN112119973A CN202011015321.0A CN202011015321A CN112119973A CN 112119973 A CN112119973 A CN 112119973A CN 202011015321 A CN202011015321 A CN 202011015321A CN 112119973 A CN112119973 A CN 112119973A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000270934 Rana catesbeiana Species 0.000 title claims abstract description 19
- 230000004083 survival effect Effects 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 239000002738 chelating agent Substances 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 239000004155 Chlorine dioxide Substances 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 235000019398 chlorine dioxide Nutrition 0.000 claims description 5
- 239000004343 Calcium peroxide Substances 0.000 claims description 4
- LHJQIRIGXXHNLA-UHFFFAOYSA-N calcium peroxide Chemical compound [Ca+2].[O-][O-] LHJQIRIGXXHNLA-UHFFFAOYSA-N 0.000 claims description 4
- 235000019402 calcium peroxide Nutrition 0.000 claims description 4
- -1 phosphate ester Chemical class 0.000 claims description 4
- 229920001503 Glucan Polymers 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 108010001062 polysaccharide-K Proteins 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 238000003908 quality control method Methods 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 6
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- 230000036541 health Effects 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 241001107116 Castanospermum australe Species 0.000 description 2
- 235000021279 black bean Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000006213 oxygenation reaction Methods 0.000 description 2
- 210000001835 viscera Anatomy 0.000 description 2
- 241000243818 Annelida Species 0.000 description 1
- 241000162910 Cacosternum Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
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- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
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- 208000010643 digestive system disease Diseases 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000003194 forelimb Anatomy 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 208000026438 poor feeding Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for improving the survival rate of bullfrog tadpoles in the early stage, which belongs to the technical field of cultivation and comprises four aspects of cultivation pond environment control, seedling release, feeding and water quality management. Aiming at the characteristics of the tadpoles of the bullfrogs in the early stage, the invention correspondingly improves the tadpole seedling selection, the breeding environment, the water quality control and the feeding placement, improves the survival rate of the tadpoles in the first 40 days to 60 percent, and obviously reduces the morbidity of the tadpoles.
Description
Technical Field
The invention relates to the technical field of breeding, in particular to a method for improving the survival rate of bullfrog tadpoles in the early stage.
Background
Bullfrog belongs to vertebrate phylum, belongs to Amphinaceae of Annelida; the meat quality is white and tender, the nutritive value is high, the taste is delicious, and the high-grade nutritional food contains high protein and low fat. The viscera and leftovers of bullfrog can be hydrolyzed into compound amino acids, and has effects of nourishing, removing toxic substances, nourishing heart, and promoting health. Besides, bullfrog can secrete various hormones, and the hormones can be extracted for medicine and industrial production.
The traditional breeding time of the bullfrog tadpoles is from clearness to midday, the air temperature and weather are unstable at this stage, so that the fluctuation of water quality and water temperature is large, the tadpoles are easy to die when the fluctuation of the temperature is more than 5 ℃, 0-40 days of the tadpoles are the key period of tadpole development, the tadpoles are weak at this stage, the low-quality substrate tadpoles are poor in eating, high in morbidity and high in death rate due to the low-fluctuation of the temperature, and the survival rate of the bullfrog tadpoles is only about 20 percent. The bullfrog tadpoles are cultured in different ponds after reaching the size of the black beans in 40 days, so the survival rate of the tadpoles in the first 40 days when the tadpoles grow into the size of the black beans has great influence on the culture benefit and the subsequent culture.
At present, in order to catch up with time and put seedlings, a considerable proportion of farmers pay little attention to the quality of the seedlings, pay little attention to the quality of tadpoles during seedling screening, buy the seedlings with small size, small heads and poor viscera development, which causes poor resistance, incomplete development of digestive organs during feeding, poor feeding, slow growth speed, high morbidity and low survival rate.
Meanwhile, when the household feeds, the feed is not fed according to the feeding and health conditions of tadpoles, and the feed is fed according to the habit of the user and the feeding rate of 3-5 kilograms for 10 ten thousand tadpoles, so that the tadpoles are periodically drunk and overeat the time; leading to staged enteritis and digestive system disease.
Meanwhile, in the existing tadpole culture, water regulating products, bottom changing products and health care products are rarely used, so that the ammonia nitrogen sub-salt in water quality exceeds standard, the ammonia nitrogen reaches more than 1mg/ml, the sub-salt is more than 0.5mg/ml, the tadpole is weak in physique, the intestinal tract, the liver and the gallbladder are unhealthy, and the death rate is high. The tadpoles are high in density, and due to few oxygen increasing measures, dissolved oxygen in the pool is within 2m/L, so that the respiration and normal physiological activities of the tadpoles are influenced, and oxygen deficiency of the tadpoles is caused. Excrement and feed residual bait at the bottom of the frog pond are less cleaned, so that the feed and the excrement residual bait are fermented at the bottom of the pond, a large amount of ammonia nitrogen and sub-salt are generated, oxygen is further consumed, and the anoxic condition is aggravated.
Therefore, improvement on the tadpole early-stage breeding mode is needed, the survival rate is improved, and the morbidity is reduced.
Disclosure of Invention
The invention aims at the problems in the prior art, provides a method for improving the survival rate of bullfrog tadpoles in the early stage, and aims at the culture of the bullfrog tadpoles within 40 days after the bullfrog tadpoles are hatched.
In order to realize the purpose of the invention, the method is realized by the following technical scheme:
a method for improving survival rate of bullfrog tadpoles in the early stage comprises the following steps:
controlling the environment of the culture pond: the water depth in the culture pond is more than or equal to 50 cm; a sunshade net is arranged above the culture pond; controlling the pH value of the pond water to be 7-8; the water temperature is 20-28 ℃; the nitrite content is less than or equal to 0.1 mg/ml; the ammonia nitrogen content is less than or equal to 0.3mg/ml, and the dissolved oxygen is more than or equal to 5 m/L;
seedling releasing: tadpoles are placed in the culture pond, and the density is 1200 and 1500 tails/m2；
Feeding: feeding feed accounting for 10-15% of tadpole weight every day; feeding for 4-6 times every day; the feeding area is less than or equal to 20 percent of the water surface area each time;
water quality management: the water change amount is less than or equal to 20 percent of the total water amount every day; and when the pH value, the water temperature, the nitrite content, the ammonia nitrogen content and the dissolved oxygen of the pond water exceed the ranges, the water change amount is increased until the pond water returns to the numerical value range.
Preferably, in the step of controlling the environment of the culture pond, an interception net is arranged on the side of the culture pond.
Preferably, in the step of controlling the environment of the culture pond, the pond water needs to be disinfected after being put into the pond water.
Preferably, in the seedling releasing step: tadpoles need to be screened, and the screening standard is that the tadpoles occupy more than or equal to 40% of the whole body.
Preferably, in the feeding step: the feeding speed is reduced by half when the feeding speed exceeds 30 minutes; until tadpoles eat within 30 minutes.
Preferably, in the feeding step: sprinkling VC phosphate ester and beta-1 and 3D glucan in the culture pond every 3 to 5 days; use of VC phosphate and beta-1, 3D glucanThe amount of each splashing is more than or equal to 10g/m3。
Preferably, in the water quality management step: and cleaning excrement and solid sundries at the bottom of the culture pond every 2 days.
Preferably, in the water quality management step: putting the calcium peroxide, the chlorine dioxide and the sodium humate chelating agent into the culture pond once every week; the adding amount of the calcium peroxide is more than or equal to 1g/m2(ii) a The adding amount of the chlorine dioxide is more than or equal to 1g/m2(ii) a The adding amount of the sodium humate chelating agent is 4-6 kg/mu.
Aiming at the characteristics of the tadpoles of the bullfrogs in the early stage, the invention correspondingly improves the tadpole seedling selection, the breeding environment, the water quality control and the feeding placement, improves the survival rate of the tadpoles in the first 40 days to 60 percent, and obviously reduces the morbidity of the tadpoles.
Detailed Description
The following specific examples are provided to further illustrate the present invention so that those skilled in the art may better understand the invention and practice it, but the examples are not intended to limit the invention. Other various modifications, substitutions and alterations of the above-described structures of the present invention will occur to those skilled in the art without departing from the basic technical spirit of the invention as described herein.
The growth stages of bullfrogs are sequentially divided into:
ovum → tadpole → long hind limb → long forelimb → young frog (tail disappearance) → adult frog.
Wherein it takes 4-5 days for the eggs to hatch into tadpoles; the tadpoles need to grow into froglets for 3-5 months; about 3 months are required for young frogs to become frogs of 0.6-0.7 jin, and about one month is required for becoming frogs of 0.6-0.7 jin to become bullfrogs which can be sold on the market.
The present invention is directed to breeding for a period of time within 0-40 days from egg hatching.
Five culture ponds are respectively numbered as No. 1, No. 2, No. 3, No. 4 and No. 5; the culture conditions and the management mode of each culture pond are the same.
Preparing the environment in the culture pond before culture:
the width of the pool is 6 meters, the length of the pool is 12 meters, and the depth of the pool is 1 meter; placing water at a depth of about 50-70 cm; intercepting nets are arranged around the culture pond; a sunshade net is arranged above the culture pond; the intercepting net and the sunshade net can play the roles of intercepting birds and sundries and properly shading sunlight.
After water is discharged, quicklime is used for disinfecting pool water, and the using amount is 100g/m2(ii) a Then controlling the pH value of the pond water to be 7-8; the water temperature is 20-28 ℃; the nitrite content is less than or equal to 0.1 mg/ml; the content of ammonia nitrogen is less than or equal to 0.3 mg/ml.
A 2KW oxygenation pump is matched in the pool; each oxygenation pump is provided with 20 air outlet pipes which are uniformly distributed in the pool, and dissolved oxygen is kept to be more than or equal to 5 m/L.
Seedling releasing:
tadpoles with large heads, black and glossy bodies and good vitality are selected as breeding seedlings; the standard of head size is that the head occupies more than 40% of the whole body length; the index of good activity is that tadpoles can move against the water flow direction.
10 thousands of tadpoles are put into each culture pond, and the equivalent density is 1388 tadpoles/m2(ii) a The seedling density can be properly adjusted and controlled at 1200-1500 tail/m2Can be implemented.
Feeding with feed:
feeding tadpoles with the weight of 10-15% on the 0-3 th day, wherein the weight is about 500 and 1000 g/pool; 0.5kg of VC phosphate and 0.5kg of beta-1 and 3D glucan are respectively sprinkled in each culture pond at 7-8 am or 4-5 pm every day;
increasing the feeding amount to 3000-5000 g/pool in 4-15 days;
increasing the feeding amount to 3000-6000 g/pool on 16-40 days;
on days 4-40, 0.5kg of VC phosphate and 0.5kg of beta-1, 3D glucan were sprinkled once in the whole pool every 3-5 days.
The feeding amount is divided into 4-6 times per day, the feeding time is at least 2 hours apart, and the area of each feeding accounts for about 20% of the water surface area. Observing the condition of tadpoles during feeding, and controlling the tadpoles to be eaten within 30 minutes; when the air temperature change amplitude is increased (more than or equal to 5 ℃), tadpoles gather to the sides of the pond, and the feeding speed exceeds 30 minutes, the feeding amount needs to be halved; until the tadpole state is recovered to normal.
The feed formula comprises the following components in parts by mass: 40 parts of fish meal, 25 parts of fermented soybean meal, 20 parts of flour, 12.8 parts of rice bran, 0.2 part of Tongwei multivitamin F11V 0.2 and 29M 2 parts of Tongwei multiore F.
The production process of the feed comprises the following steps: uniformly mixing all the components, and then performing puffing granulation; then carrying out superfine grinding; pulverizing, and sieving with 80 mesh sieve.
Monitoring water quality:
after seedling release, the water amount is changed to be 20% of the total water amount every day, and the pH value, the water temperature, the nitrite content, ammonia nitrogen and dissolved oxygen are monitored in real time; when the index is out of range, the water exchange amount is increased until the index is recovered to be normal.
And cleaning the excrement at the bottom of the culture pond every 2 days.
After 3 days of seedling release, the dosage of using chlorine dioxide to disinfect once per week is 0.5g/m2(ii) a The dosage of calcium peroxide is 1g/m2(ii) a (ii) a Using a sodium humate chelating agent to purify debris and germs in the water body every week; the amount of the sodium humate chelating agent is 4-6 kg/mu
After 40 days of culture, the survival rates of the culture ponds 1, 2, 3, 4 and 5 are 72%, 65%, 70%, 66% and 69% respectively; the average survival rate is 68.4%.
Claims (8)
1. A method for improving survival rate of bullfrog tadpoles in the early stage is characterized by comprising the following steps:
controlling the environment of the culture pond: the water depth in the culture pond is more than or equal to 50 cm; a sunshade net is arranged above the culture pond; controlling the pH value of the pond water to be 7-8; the water temperature is 20-28 ℃; the nitrite content is less than or equal to 0.1 mg/ml; the ammonia nitrogen content is less than or equal to 0.3mg/ml, and the dissolved oxygen is more than or equal to 5 m/L;
seedling releasing: tadpoles are placed in the culture pond, and the density is 1200 and 1500 tails/m2；
Feeding: feeding feed accounting for 10-15% of tadpole weight every day; feeding for 4-6 times every day; the feeding area is less than or equal to 20 percent of the water surface area each time;
water quality management: the water change amount is less than or equal to 20 percent of the total water amount every day; and when the pH value, the water temperature, the nitrite content, the ammonia nitrogen content and the dissolved oxygen of the pond water exceed the ranges, the water change amount is increased until the pond water returns to the numerical value range.
2. The method of claim 1, wherein said method comprises the steps of: in the step of controlling the environment of the culture pond, an interception net is arranged on the side of the culture pond.
3. The method of claim 1, wherein said method comprises the steps of: in the step of controlling the environment of the culture pond, the pond water needs to be disinfected after being put into the pond water.
4. The method of claim 1, wherein said method comprises the steps of: the seedling releasing step comprises: tadpoles need to be screened, and the screening standard is that the tadpoles occupy more than or equal to 40% of the whole body.
5. The method of claim 1, wherein said method comprises the steps of: the feeding step comprises the following steps: the feeding speed is reduced by half when the feeding speed exceeds 30 minutes; until tadpoles eat within 30 minutes.
6. The method of claim 1, wherein said method comprises the steps of: the feeding step comprises the following steps: sprinkling VC phosphate ester and beta-1 and 3D glucan in the culture pond every 3 to 5 days; the dosage of VC phosphate ester and beta-1, 3D glucan is not less than 10g/m3。
7. The method of claim 1, wherein said method comprises the steps of: the water quality management step comprises: and cleaning excrement and solid sundries at the bottom of the culture pond every 2 days.
8. The method of claim 1, wherein said method comprises the steps of: the water quality management step comprises: putting the calcium peroxide, the chlorine dioxide and the sodium humate chelating agent into the culture pond once every week; said peroxygenThe adding amount of calcium is more than or equal to 1g/m2(ii) a The adding amount of the chlorine dioxide is more than or equal to 1g/m2(ii) a The adding amount of the sodium humate chelating agent is 4-6 kg/mu.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011015321.0A CN112119973A (en) | 2020-09-24 | 2020-09-24 | Method for increasing survival rate of bullfrog tadpoles in early stage |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011015321.0A CN112119973A (en) | 2020-09-24 | 2020-09-24 | Method for increasing survival rate of bullfrog tadpoles in early stage |
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| CN202011015321.0A Pending CN112119973A (en) | 2020-09-24 | 2020-09-24 | Method for increasing survival rate of bullfrog tadpoles in early stage |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0196879A2 (en) * | 1985-03-26 | 1986-10-08 | Shuichi Urata | Method of producing hairless pigs |
| CN102485020A (en) * | 2011-09-30 | 2012-06-06 | 湛江腾飞实业有限公司 | Soft feed for babylonia areolata parent mature stage and preparation method thereof |
| CN104068268A (en) * | 2014-06-24 | 2014-10-01 | 江苏明辉饲料有限公司 | Compound feed for breeding penaeus vannamei boone in greenhouse |
| CN105594649A (en) * | 2016-01-26 | 2016-05-25 | 中国中医科学院中药研究所 | Tadpole culturing jar and culturing method for improving metamorphosis rate of Bufo gargarizans tadpoles |
| CN107173324A (en) * | 2017-06-29 | 2017-09-19 | 杨慧 | It is a kind of to improve the method that frog cultivates period of metamorphosis survival rate |
| CN107373045A (en) * | 2017-07-27 | 2017-11-24 | 张银花 | One kind prevention leukodermal solid feed of lobster and preparation method thereof |
-
2020
- 2020-09-24 CN CN202011015321.0A patent/CN112119973A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0196879A2 (en) * | 1985-03-26 | 1986-10-08 | Shuichi Urata | Method of producing hairless pigs |
| CN102485020A (en) * | 2011-09-30 | 2012-06-06 | 湛江腾飞实业有限公司 | Soft feed for babylonia areolata parent mature stage and preparation method thereof |
| CN104068268A (en) * | 2014-06-24 | 2014-10-01 | 江苏明辉饲料有限公司 | Compound feed for breeding penaeus vannamei boone in greenhouse |
| CN105594649A (en) * | 2016-01-26 | 2016-05-25 | 中国中医科学院中药研究所 | Tadpole culturing jar and culturing method for improving metamorphosis rate of Bufo gargarizans tadpoles |
| CN107173324A (en) * | 2017-06-29 | 2017-09-19 | 杨慧 | It is a kind of to improve the method that frog cultivates period of metamorphosis survival rate |
| CN107373045A (en) * | 2017-07-27 | 2017-11-24 | 张银花 | One kind prevention leukodermal solid feed of lobster and preparation method thereof |
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