CN112119834A - High-activity ganoderma lucidum culture medium - Google Patents
High-activity ganoderma lucidum culture medium Download PDFInfo
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- CN112119834A CN112119834A CN202010975799.1A CN202010975799A CN112119834A CN 112119834 A CN112119834 A CN 112119834A CN 202010975799 A CN202010975799 A CN 202010975799A CN 112119834 A CN112119834 A CN 112119834A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a high-activity ganoderma lucidum culture medium, which comprises 50-90% of blood-nourishing and nerve-soothing granule medicine residues, 5-45% of bran, 0.5-1% of yeast powder, 1-3% of small molecular peptide, 1% of gypsum, 1% of granulated sugar and KH2PO40.2 percent and the ratio of material to water is 1:1.5, thus solving the problem of recycling the solid waste of the traditional Chinese medicine residue, comprehensively utilizing the residue and reducing the production cost of the ganoderma lucidum. Meanwhile, the ganoderma lucidum sporocarp effectively utilizes the effective components of the blood-nourishing and nerve-soothing granule dregs, so that the activity of ganoderma lucidum is effectively improved, and the Chinese medicine dregs are changed into valuables.
Description
Technical Field
The invention relates to the field of edible fungus culture, and particularly relates to a high-activity ganoderma lucidum culture medium.
Background
Ganoderma belongs to dry fruiting body of Ganoderma lucidum or Ganoderma sinensis of Polyporaceae of Basidiomycetes, is a large-scale fungus, is called as "Mesona chinensis and" Ruilao chinensis ", is a traditional rare medicinal fungus in China since ancient times, and has the effects of nourishing and strengthening, and strengthening body resistance and consolidating constitution.
Modern medical research shows that ganoderma contains a large amount of bioactive substances, such as ganoderma polysaccharide, triterpene, alkaloid, protein and the like, and has the effects of enhancing immunity, promoting metabolism, resisting tumors, protecting liver, improving memory, promoting sleep, delaying senescence and the like. The ganoderan is used as the main bioactive component of ganoderma, exists in natural ganoderma sporocarp and mycelium, and has strong bioactivity of resisting tumor, resisting oxygen free radical, resisting aging, improving immunity, promoting blood circulation, removing blood stasis, etc.
The main mode of modern industrialized production of ganoderma lucidum is artificial planting in a greenhouse. The culture medium mainly comprises corn straws, xylitol residues, bran, soybean cake powder, gypsum powder and the like. Not only the culture medium is expensive, but also trace heavy metal salts often exist in the culture medium to influence the quality of the lucid ganoderma.
The Chinese medicine dregs are the leftover of Chinese patent medicine production enterprises, and a large amount of nutritional active ingredients are not utilized. The traditional Chinese medicine residues are used as solid garbage of pharmaceutical enterprises, and unreasonable treatment not only faces serious net pressure, but also increases the production cost of the enterprises. The rational utilization of herb dregs is urgent. The blood-nourishing and nerve-soothing granules are prepared from rare raw materials such as hairyvein agrimony, prepared rehmannia root, vine of multiflower knotweed, yerbadetajo herb, rehmannia root, suberect spatholobus stem and the like through processing and have the functions of nourishing yin and blood, calming heart and soothing nerves and the like.
Disclosure of Invention
The invention aims to provide a high-activity ganoderma lucidum culture medium, which can not only improve the yield of ganoderma lucidum, but also enhance the effective active ingredients of ganoderma lucidum, so that the nutritive values of ganoderma lucidum fruiting bodies and mycelia obtained by culture are richer.
Secondly, the invention provides a utilization method of the blood nourishing and nerve calming granule dregs, which recycles the blood nourishing and nerve calming granule dregs, further develops the utilization value of the dregs, reduces the environmental pollution and better increases the pharmacological activity of the ganoderma lucidum.
The innovation point of the invention is mainly that the traditional ganoderma lucidum culture mode is broken through, and the traditional Chinese medicine residues are used as ganoderma lucidum culture raw materials, so that waste is changed into valuable. Meanwhile, the ganoderma lucidum effectively utilizes the effective components of the Chinese medicine residues, so that the content of ganoderma lucidum polysaccharide, triterpene and the like finally cultured is increased, and the pharmacological activity of the ganoderma lucidum is increased.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a high-activity culture medium for Ganoderma comprises (by weight) medicinal residue of blood nourishing and tranquilizing granule 50-90%, testa Tritici 5-45%, yeast powder 0.5-1%, small molecular peptide 1-3%, Gypsum Fibrosum 1%, granulated sugar 1%, and KH2PO40.2 percent and the ratio of material to water is 1: 1.5.
The blood nourishing and nerve soothing granules comprise the following components: prepared with agrimony, prepared rehmannia root, vine of multiflower knotweed, yerbadetajo herb, rehmannia root, spatholobus stem and other noble materials.
Obtaining blood-nourishing and nerve-soothing granule dregs: water content 8-10%, pH6.5-7.4, crude protein 0.9-1.2%, crude fiber 60-65%, soluble carbohydrate 21.2-25.8%, and heavy metal not detected.
The invention provides a culture medium for producing lucid ganoderma by taking blood-nourishing and nerve-soothing granular medicine residues as main raw materials. On one hand, the invention solves the problem of recycling the solid waste of the traditional Chinese medicine residue, comprehensively utilizes the medicinal residue and reduces the production cost of the lucid ganoderma. Meanwhile, the ganoderma lucidum sporocarp effectively utilizes the effective components of the blood-nourishing and nerve-soothing granule dregs, so that the activity of ganoderma lucidum is effectively improved, and the Chinese medicine dregs are changed into valuables.
Drawings
FIG. 1 shows the extraction method of ganoderan.
FIG. 2 shows the steps of ganoderic acid extraction.
Detailed Description
Embodiments of the present invention will be described in detail with reference to examples. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
Culturing ganoderma lucidum mycelia:
korean Ganoderma strain is stored in life science institute of chat university.
1) Strain activation
Cutting 200g peeled potato into small pieces, adding water, boiling for 20min, and filtering with gauze to obtain filtrate; adding 20g of glucose and 20g of agar into the filtrate, and metering to 1L; packaging into test tubes, sterilizing at 121 deg.C for 20min, cooling to room temperature, and making into slant. Inoculating Ganoderma mycelia in the slant with two rings.
2) And (5) culturing sporocarps.
Comprises 55 wt% of Chinese medicinal residue, 40 wt% of bran, 0.5 wt% of yeast powder, 1.5 wt% of soybean peptide, 0.8 wt% of oyster peptide, 0.8 wt% of granulated sugar, 1 wt% of gypsum and KH2PO40.2 percent and the ratio of material to water is 1: 1.5. Packaging with polypropylene bag, and adjusting pH. Obtaining a fungus bag, and sterilizing for later use. Inoculating the mycelium in the slant to the fungus bag, and culturing.
3) Component detection
And (3) observing hypha morphology: uneven growth, faster growth in the early stage and slower growth in the middle and later stages. And pigment secretion forms sporadic color spots.
The analysis of Ganoderma Lucidum Polysaccharide (GLP) shows that the solid mycoplasm polysaccharide content is 2.5% and the fruiting body polysaccharide content is 0.85%. The extraction method of ganoderan is shown in figure 1, and the determination method adopts phenol-sulfuric acid method.
Analysis of water-soluble protein content: 2.4 percent. The protein component determination method adopts Coomassie brilliant blue method.
Analysis of ganoderic acid triterpene content: 4.6 percent. The extraction of ganoderic acid product is shown in FIG. 2, and the content of ganoderic acid triterpene is analyzed by spectrophotometry (250 nm).
Example 2
Basically the same as in example 1. The difference lies in that:
1) ganoderma lucidum cultivation
65% of culture medium blood-nourishing and nerve-soothing granule dregs, 30% of bran, 0.5% of yeast powder, 1.5% of soybean peptide, 0.8% of oyster peptide, 1% of gypsum, 1% of granulated sugar and KH2PO4 0.2%。
2) Component detection
And (3) observing hypha morphology: the growth situation is basically consistent, and the hyphae are thick and strong. A small amount of yellow spots appeared.
Analysis of Ganoderma Lucidum Polysaccharide (GLP) shows that solid mycoplasm polysaccharide content is 3.2%, and fruiting body polysaccharide content is 1.28%.
Analysis of water-soluble protein content: 3.5 percent.
Analysis of ganoderic acid triterpene content: 5.3 percent.
Example 3
Basically the same as in example 1. The difference lies in that:
1) ganoderma lucidum cultivation
The culture medium comprises 70% of blood-nourishing and nerve-soothing granule dregs, 25% of bran, 0.5% of yeast powder, 1.5% of soybean peptide, 0.8% of oyster peptide, 1% of gypsum, 1% of granulated sugar and KH2PO40.2%.
2) Component detection
And (3) observing hypha morphology: the growth situation is basically consistent, and the hyphae are dense, strong and white. Five macroscopic yellow spots.
Analysis of Ganoderma Lucidum Polysaccharide (GLP) shows that solid mycoplasm polysaccharide content is 3.6%, and fruiting body polysaccharide content is 1.59%.
Analysis of water-soluble protein content: 3.9 percent.
Analysis of ganoderic acid triterpene content: 5.5 percent.
Example 4 is essentially the same as example 1. The difference lies in that:
1) ganoderma lucidum cultivation
The culture medium comprises 80% of blood-nourishing and nerve-soothing granule dregs, 15% of bran, 0.5% of yeast powder, 1.5% of soybean peptide, 0.8% of oyster peptide, 1% of gypsum, 1% of granulated sugar and KH2PO40.2%.
2) Component detection
And (3) observing hypha morphology: the growth situation is basically consistent, and the hyphae are dense, strong and white. Five macroscopic yellow spots.
Analysis of Ganoderma Lucidum Polysaccharide (GLP) shows that the solid mycoplasm polysaccharide content is 3.8%, and the fruiting body polysaccharide content is 1.79%.
Analysis of water-soluble protein content: 3.4 percent.
Analysis of ganoderic acid triterpene content: 5.1 percent.
Claims (3)
1. A high-activity ganoderma lucidum culture medium is characterized by comprising 50-90% of blood-nourishing and nerve-soothing granule medicine residues, 5-45% of bran and 0.5-1% of yeast powder by mass percent,1-3% of small molecular peptide, 1% of gypsum, 1% of granulated sugar and KH2PO40.2 percent and the ratio of material to water is 1: 1.5.
2. The high-activity ganoderma lucidum culture medium of claim 1, wherein the blood-nourishing and nerve-soothing granular decoction dregs contain 8-10% of water, 6.5-7.4% of pH, 0.9-1.2% of crude protein, 60-65% of crude fiber, 21.2-25.8% of soluble carbohydrate and no heavy metal detected.
3. The high-activity ganoderma lucidum culture medium according to claim 1, wherein the small molecular peptide is one or more of soybean peptide, peanut peptide or oyster peptide.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114009274A (en) * | 2021-12-02 | 2022-02-08 | 浙江寿仙谷医药股份有限公司 | Moisturizing synergistic ganoderma lucidum planting matrix and preparation method and efficient recycling method thereof |
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Application publication date: 20201225 |