CN112088881B - Application of 8-methoxy kaempferol in preparation of preparation for promoting plant growth and improving salt tolerance of plants - Google Patents
Application of 8-methoxy kaempferol in preparation of preparation for promoting plant growth and improving salt tolerance of plants Download PDFInfo
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- CN112088881B CN112088881B CN202010854404.2A CN202010854404A CN112088881B CN 112088881 B CN112088881 B CN 112088881B CN 202010854404 A CN202010854404 A CN 202010854404A CN 112088881 B CN112088881 B CN 112088881B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses application of 8-methoxy kaempferol in preparation of a preparation for promoting plant growth and improving plant salt tolerance. When the 8-methoxy kaempferol is applied to the plant seeds stressed by salt, the germination rate and the germination speed of the seeds under the salt stress can be obviously improved; can obviously improve the plant height, root length and fresh weight of the plant seedlings under the salt stress and improve the salt tolerance of the plants. Growth can be promoted by applying an aqueous solution of 8-methoxykaempferol under normal conditions; the application of the 8-methoxy kaempferol aqueous solution under the condition of salt stress can improve the seed germination rate and the germination potential and reduce the salt damage; can also improve the height, the root length, the fresh weight and the survival rate of the plants, and can achieve the purposes of protecting and strengthening the seedlings and improving the salt stress resistance in production. The application method disclosed by the invention is simple to use and has an obvious effect.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to application of 8-methoxy kaempferol in preparation of a preparation for promoting plant growth and improving plant salt tolerance.
Background
8-methoxy kaempferol with the structural formula
Is a flavonoid polyphenol compound widely existing in tea, broccoli, delphinium, hamamelis, grapefruit, brussel sprout, apple, etc. Kaempferol is one of the bioflavonoids present at high levels in cruciferous vegetables. Kaempferol has a wide range of pharmacological activities including anticancer, cardioprotective, neuroprotective, antidiabetic, antiosteoporosis, antiestrogen, anxiolytic, analgesic, antiallergic, antiinflammatory and antibacterial effects. Research has confirmed that it has a number of functions, such as: is useful for preventing heart disease and cancer, and is a potential anticancer agentAn agent; can prevent oxidation of bone cells in vitro and may exert an anti-osteoporosis effect; can inhibit Fatty Acid Synthetase (FASN), and is an anti-obesity drug. However, no research report on the plant stress resistance of kaempferol is found at present.
Soil salination is an important factor affecting the yield and quality of crops today. Excess salt ions cause damage to plants primarily by causing osmotic stress, ionic toxicity and oxidative stress, ultimately resulting in a reduction in crop economic yield. How to enhance the salt resistance of plants is a hot spot in the research of plant salt resistance mechanism. The method for improving the stress resistance of the plants by applying the compound externally is simple and convenient.
Disclosure of Invention
Aiming at the prior art situation, the invention aims to provide the application of 8-methoxy kaempferol in preparing a preparation for promoting plant growth and improving plant salt tolerance, and can achieve the purposes of protecting and strengthening seedlings and improving salt stress resistance in production.
In order to realize the purpose of the invention, the invention is realized by adopting the following technical scheme:
the invention provides application of 8-methoxy kaempferol in preparation of a preparation for promoting plant growth.
The invention also provides application of the 8-methoxy kaempferol in preparation of a preparation for improving salt tolerance of plants.
Further, the specific application method is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings.
Further, the application comprises the following specific steps:
sterilizing the surface of the plant seeds with ethanol, washing with sterile water, and soaking the plant seeds in the aqueous solution of the preparation; or soaking the roots of young plants in the aqueous solution of the preparation.
Further, the concentration of the 8-methoxy kaempferol in the water solution of the preparation is 0.4-0.7 mu mol/L.
Further, the plant seeds are soaked for 12-16 h; the soaking time of the roots of the plant seedlings is 4 to 7 days.
Further, the plant seeds or seedlings are seeds or seedlings of wheat, millet and tobacco.
Furthermore, the 8-methoxy kaempferol can improve the germination rate of seeds, the germination potential of the seeds, the bud length of seedlings, the root length of the seedlings, the fresh weight of the seedlings and the survival rate of the seedlings.
Compared with the prior art, the invention has the advantages and positive effects that: the invention provides application of 8-methoxy kaempferol in preparation of a preparation for promoting plant growth and improving plant salt tolerance. 8-methoxy kaempferol can act in plants as a plant metabolite. Growth can be promoted by applying an aqueous solution of 8-methoxykaempferol under normal conditions; the application of the 8-methoxy kaempferol aqueous solution under the condition of salt stress can improve the seed germination rate and the germination potential and reduce the salt damage; can also improve the height, the root length, the fresh weight and the survival rate of the plants, and can achieve the purposes of protecting and strengthening the seedlings and improving the salt stress resistance in production. The application method disclosed by the invention is simple to use and has an obvious effect.
Drawings
FIG. 1 is a graph showing the growth promoting effect of 8-methoxykaempferol on seeds; a: growth promoting effect of 8-methoxy kaempferol on wheat seeds, B: growth promoting effect of 8-methoxy kaempferol on millet seeds, C: the growth promotion effect of the 8-methoxy kaempferol on the tobacco seeds;
FIG. 2 is a graph showing the growth promoting effect of 8-methoxykaempferol on wheat seedlings;
FIG. 3 is a graph showing the growth promoting effect of 8-methoxykaempferol on millet seedlings;
FIG. 4 is a graph showing the growth promoting effect of 8-methoxykaempferol on tobacco seedlings;
FIG. 5 shows the effect of 8-methoxy kaempferol in promoting salt tolerance of seeds; a: the promotion effect of 8-methoxy kaempferol on the salt tolerance of wheat seeds, B: the salt tolerance promotion effect of 8-methoxy kaempferol on millet seeds is shown in the specification, wherein C: the 8-methoxyl kaempferol has the function of promoting the salt tolerance of tobacco seeds;
FIG. 6 shows the effect of 8-methoxykaempferol in promoting salt tolerance of wheat seedlings;
FIG. 7 shows the effect of 8-methoxykaempferol in promoting salt tolerance of millet seedlings;
FIG. 8 shows the effect of 8-methoxykaempferol in promoting the salt tolerance of tobacco seedlings.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and the detailed description.
Example 1, 8-methoxy kaempferol can promote plant growth: seed germination and seedling growth
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. And (3) soaking the seeds of the control group and the experimental group in corresponding treatment solutions for 12 hours respectively for pretreatment. Three replicates of each treatment, each 50 seeds in parallel.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. And counting germination data every day, and counting bud length, root length and fresh weight. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 1 growth promoting Effect of 8-Methoxybanediol on wheat, millet and tobacco seeds
Note: p is less than 0.001, P is less than 0.01, P is less than 0.05
In Table 1, CK is distilled water and CK + 0.5. mu.M SX is an aqueous solution containing 0.5. mu.M 8-methoxykaempferol. Germination rate is 100% of the number of germinated seeds/total number of seeds. Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination. .
As can be seen from Table 1 and A, B, C of FIG. 1, exogenous application of 8-methoxykaempferol significantly promoted germination and post-germination growth of plant seeds compared to controls.
2. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and carrying out corresponding solution treatment. And (5) observing the phenotype. And counting the bud length, the root length and the fresh weight.
As can be seen from fig. 2, 3 and 4, exogenous application of 8-methoxykaempferol under normal conditions significantly promoted the growth of plants, compared to the control.
Example 2, 8-Methoxypentanoic acid Kaempferol can improve the salt tolerance of plant seeds
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. The seeds of the control group and the experimental group are respectively soaked in the treatment solution for 12 hours for pretreatment. Three replicates of each treatment, 50 seeds per replicate.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. Germination data were counted daily. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 2 salt tolerance promotion of 8-methoxy kaempferol on wheat, millet and tobacco seeds
Note: p is less than 0.001, P is less than 0.01 and P is less than 0.05.
In Table 2, NaCl was 150mM, and NaCl + 0.5. mu.M SX was a NaCl solution containing 0.5. mu.M 8-methoxykaempferol. Germination rate is 100% of the number of germinated seeds/total number of seeds. Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination.
It is clear from table 2 and A, B, C of fig. 5 that exogenous 8-methoxy kaempferol pretreatment significantly promoted germination and growth of wheat, millet and tobacco seeds under salt stress as compared to NaCl stress alone.
Example 3, 8-Methoxypentanediol can improve the salt tolerance of young plants
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and soaking the seedlings in 8-methoxy kaempferol aqueous solution. And (5) observing the phenotype. And counting the bud length, the root length, the fresh weight and the survival rate.
As can be seen from fig. 6, 7 and 8, exogenous application of 8-methoxykaempferol under salt stress conditions significantly promoted the growth of plants, compared to the control.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (5)
- The application of 8-methoxyl kaempferol in preparing a preparation for promoting plant growth is characterized in that: the specific application mode is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings; the plant seeds or seedlings are seeds or seedlings of wheat, millet and tobacco; the concentration of 8-methoxy kaempferol in the aqueous solution of the preparation is 0.5 mu mol/L.
- The application of 8-methoxy kaempferol in preparing the preparation for improving the salt tolerance of plants is characterized in that: the specific application mode is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings; the plant seeds or seedlings are seeds or seedlings of wheat, millet and tobacco; the concentration of 8-methoxy kaempferol in the aqueous solution of the preparation is 0.5 mu mol/L.
- 3. Use according to claim 1 or 2, characterized in that: the application comprises the following specific steps:sterilizing the surface of the plant seeds with ethanol, washing with sterile water, and soaking the plant seeds in the aqueous solution of the preparation; or soaking the roots of young plants in the aqueous solution of the preparation.
- 4. Use according to claim 3, characterized in that: the soaking time of the plant seeds is 12-16 h; the soaking time of the roots of the plant seedlings is 4 to 7 days.
- 5. Use according to claim 1 or 2, characterized in that: the 8-methoxy kaempferol can improve the germination rate of seeds, the germination potential of the seeds, the bud length of seedlings, the root length of seedlings, the fresh weight of the seedlings and the survival rate of the seedlings.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105050402A (en) * | 2013-03-27 | 2015-11-11 | 诺维信生物农业公司 | Compositions and methods for enhancing plant growth |
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN107926199A (en) * | 2017-11-18 | 2018-04-20 | 吕强 | A kind of tomato seeds disease-resistant treatment method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN105050402A (en) * | 2013-03-27 | 2015-11-11 | 诺维信生物农业公司 | Compositions and methods for enhancing plant growth |
| CN107926199A (en) * | 2017-11-18 | 2018-04-20 | 吕强 | A kind of tomato seeds disease-resistant treatment method |
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