CN110326622B - Composition capable of enhancing drought resistance of cotton seedlings and drought resistance method - Google Patents

Composition capable of enhancing drought resistance of cotton seedlings and drought resistance method Download PDF

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CN110326622B
CN110326622B CN201910664555.9A CN201910664555A CN110326622B CN 110326622 B CN110326622 B CN 110326622B CN 201910664555 A CN201910664555 A CN 201910664555A CN 110326622 B CN110326622 B CN 110326622B
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cotton
composition
drought resistance
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CN110326622A (en
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庞朝友
张小萌
刘绍东
赵新华
葛常伟
陈静
张思平
沈倩
马慧娟
刘瑞华
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/50—Cotton
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a composition capable of enhancing drought resistance of cotton seedlings and a drought resistance method, wherein the composition comprises nomilin and hesperetin, the mol ratio of the nomilin to the hesperetin is 0.5-1.5 mu mol: 1-3 mu mol. The composition has an antioxidation effect, can remove free radicals, superoxide anion free radicals and peroxides, resist lipid peroxidation and linoleic acid oxidation, and simultaneously has a certain protection effect on DNA damage; the cotton seedlings treated by the method can improve the survival rate and enhance the drought resistance of the cotton seedlings under drought stress, and the method has the advantages of low cost, simple operation and strong popularization, can be used in the actual cotton production, and can effectively solve the problem of insufficient water resources on the premise of ensuring the cotton yield.

Description

Composition capable of enhancing drought resistance of cotton seedlings and drought resistance method
Technical Field
The invention belongs to the technical field of new crop variety cultivation, and particularly relates to a composition capable of enhancing drought resistance of cotton seedlings and a drought resistance method.
Background
Cotton is the most important strategic material in China except for grains. The cotton production area of China is further concentrated to the Xinjiang cotton area in the northwest inland by the original three cotton areas of the inland cotton area in the northwest, the cotton area in the yellow river basin and the cotton area in the Yangtze river basin. Xinjiang is one of the most serious provinces of the phenomenon of land competition between cotton and grain, and water resources are seriously insufficient. Researches show that drought stress can destroy the metabolic balance of active oxygen in plants to cause peroxidation of cell membrane lipid; drought stress can also cause leaf wilting, stomata closing, damage chloroplast structure, result in reduced plant photosynthesis, reduced photosynthetic rate, and ultimately affect yield. Therefore, the method for enhancing the drought resistance of the cotton has important significance for maintaining the active oxygen balance in the cotton body and ensuring the normal photosynthesis.
Research shows that no specific measures or substances for enhancing the drought resistance of cotton exist in the prior production except for a transgenic means. The cotton transgenic period is long, the technology is not completely mature, and the popularization in production is difficult. After the plants are stressed by drought, the content of active oxygen in the plants can be rapidly increased, and serious toxic effects can be generated on cells.
The present invention has been made in view of the above circumstances.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a composition capable of enhancing the drought resistance of cotton seedlings and a drought resistance method.
In order to achieve the purpose, the invention adopts the following technical scheme:
a composition for enhancing drought resistance of cotton seedlings, said composition comprising nomilin and hesperetin in a molar ratio of between 0.5 and 1.5 μmol: 1-3 mu mol.
Further, the mol ratio of the nomilin to the hesperetin is 1 mu mol: 2. mu. mol.
A method for enhancing drought resistance of cotton seedlings by using the composition comprises the following steps:
(1) firstly, selecting test cotton seeds, then sowing and watering;
(2) spraying or irrigating the ethanol solution of the composition for root after cotton seedling grows out, continuously spraying or irrigating the root for 3 days, and spraying or irrigating the root once in the morning and evening.
Further, the cotton seeds in the step (1) are soaked for 16-24 hours in the soaking liquid.
Further, the seed soaking liquid comprises, by weight, 100 parts of water, 0.4-0.8 part of salicylic acid, 4-8 parts of aspartic acid and 6-10 parts of anthocyanin.
Further, the seed soaking solution comprises 100 parts by weight of water, 0.6 part by weight of salicylic acid, 6 parts by weight of aspartic acid and 8 parts by weight of anthocyanin.
The drought resistance of cotton seedlings can be obviously enhanced by soaking cotton seeds in the seed soaking liquid, and the germination rate, the germination vigor and the seedling quality of cotton are obviously improved under the drought condition. The salicylic acid and the anthocyanin have the functions of resisting oxidation, removing endogenous free radicals and effectively improving the drought resistance of plants, and the aspartic acid can increase the comprehensive absorption of nitrogen, phosphorus, potassium and other trace elements by crops and is beneficial to seed germination.
Further, spraying or irrigating the composition when the seedlings grow to 4-6cm in the step (2).
Further, the concentration of nomilin in the ethanol solution of the composition is 0.5-1.5 mu mol.L-1The concentration of hesperetin is 1-3 mu mol.L-1。
Further, 6-14ml of ethanol solution of the composition is sprayed or irrigated to roots of each cotton seedling in the step (2) every time.
Further, 9-11ml of ethanol solution of the composition is sprayed or irrigated to roots of each cotton seedling in the step (2) every time.
Further, 10ml of ethanol solution of the composition is sprayed or irrigated to roots of each cotton seedling in the step (2) every time.
The hesperetin is a flavone, belongs to a secondary metabolite, and the secondary metabolite can be used as an osmotic regulator to play an important role in regulating the drought resistance of plants.
Nomlein is a limonin analogue aglycone and has certain antioxidant capacity. Animal experiments show that the compound can obviously reduce the levels of lipofuscin and malondialdehyde in serum and brain tissues of an aging model mouse, and can also improve the activity of glutathione peroxidase and superoxide dismutase in the serum and the brain tissues and the total antioxidant capacity in the brain tissues. The inventor discovers through a large number of tests that the drought resistance of cotton seedlings can be enhanced under the combined action of nomilin and hesperetin, and the drought resistance effect of the two is obviously enhanced when the nomilin and the hesperetin are used together compared with that of one when the nomilin and the hesperetin are used alone.
Ethanol solution preparation of the composition of the invention: firstly, preparing 5 mmol.L of nomilin and hesperetin respectively by using absolute ethyl alcohol-1The mother liquor is subpackaged and stored in a refrigerator at the temperature of minus 20 ℃. During the test, the mother liquor is diluted to the required concentration by water according to the molar ratio by adopting a concentration gradient dilution method.
The invention has the beneficial effects that:
(1) the composition has an antioxidation effect, can remove free radicals, superoxide anion free radicals and peroxides, resist lipid peroxidation and linoleic acid oxidation, has a certain protection effect on DNA damage, and plays a drought resisting role in cotton seedlings;
(2) the cotton seedlings treated by the method can improve the survival rate and enhance the drought resistance of the cotton seedlings under drought stress, and the method has the advantages of low cost, simple operation and strong popularization, can be used in the actual cotton production, and can effectively solve the problem of insufficient water resources on the premise of ensuring the cotton yield.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
In the embodiment of the invention, Xinluo No. 9 is selected as the cotton seed, the orange peel manufacturer is Shanghai-sourced leaf Biotechnology limited company, the purity of the reagent is more than or equal to 95%, the Nomlein manufacturer is Shanghai-sourced leaf Biotechnology limited company, and the purity of the reagent is more than or equal to 98%.
Ethanol solution formulation of the compositions described in the examples of the invention: firstly, preparing 5 mmol.L of nomilin and hesperetin by using absolute ethyl alcohol respectively-1The mother liquor is subpackaged and stored in a refrigerator at the temperature of minus 20 ℃. During the test, the mother liquor is diluted to the required concentration by water according to the molar ratio by adopting a concentration gradient dilution method.
Example 1
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has a molar ratio of nomilin to hesperetin of 0.5 mu mol: 1. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly selecting test cotton seeds, then sowing the test cotton seeds in a room-temperature seedling raising box, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) spraying ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 0.5 mu mol.L-1The concentration of hesperetin is 1 mu mol.L-1The composition is continuously sprayed for 3 days, and is sprayed once in the morning and at night, and 6ml of the composition is sprayed to each cotton seedling.
Example 2
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has the molar ratio of nomilin to hesperetin of 1 mu mol: 2. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly selecting test cotton seeds, then sowing the test cotton seeds in a room-temperature seedling raising box, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) spraying ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 1 mu mol.L-1The concentration of hesperetin is 2 mu mol.L-1The composition is continuously sprayed for 3 days, and is sprayed once in the morning and at night, and 10ml of the composition is sprayed to each cotton seedling.
Example 3
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has a molar ratio of nomilin to hesperetin of 1.5 mu mol: 3. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly selecting test cotton seeds, then sowing the test cotton seeds in a room-temperature seedling raising box, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) irrigating the root of the ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 1.5 mu mol/L-1The concentration of hesperetin is 3 mu mol.L-1And continuously irrigating roots for 3 days, irrigating roots once in the morning and at night, and irrigating roots of each cotton seedling by 10ml of the composition.
Example 4
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has a molar ratio of nomilin to hesperetin of 0.6 mu mol: 1.5. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly selecting test cotton seeds, soaking the cotton seeds in a seed soaking solution for 16 hours, according to 10kg of the seed soaking solution, wherein the seed soaking solution comprises 100 parts by weight of water, 0.4 part by weight of salicylic acid, 4 parts by weight of aspartic acid and 6 parts by weight of anthocyanin, then sowing the seeds in seedling raising boxes at room temperature, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) spraying ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 0.6 mu mol.L-1The concentration of hesperetin is 1.5 mu mol.L-1The composition is continuously sprayed for 3 days, and is sprayed once in the morning and at night, and 11ml of the composition is sprayed to each cotton seedling.
Example 5
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has a mol ratio of nomilin to hesperetin of 1.2 mu mol: 2.5. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly, selecting test cotton seeds, soaking the cotton seeds in a seed soaking solution for 20 hours, according to 10kg of the seed soaking solution, wherein the seed soaking solution comprises 100 parts by weight of water, 0.6 part by weight of salicylic acid, 6 parts by weight of aspartic acid and 8 parts by weight of anthocyanin, then sowing the seeds in seedling raising boxes at room temperature, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) irrigating the root of the ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 1.2 mu mol/L-1The concentration of hesperetin is 2.5 mu mol.L-1And continuously irrigating roots for 3 days, irrigating the roots once in the morning and at the evening, and irrigating the roots of each cotton seedling with 12ml of the composition each time.
Example 6
The composition for enhancing the drought resistance of cotton seedlings in the embodiment has the molar ratio of nomilin to hesperetin of 1 mu mol: 2.5. mu. mol.
The method for enhancing the drought resistance of cotton seedlings by using the composition prepared by the embodiment comprises the following steps:
(1) firstly selecting test cotton seeds, soaking the cotton seeds in a seed soaking solution for 24 hours, then sowing the test cotton seeds according to 10kg of the seed soaking solution, wherein the seed soaking solution comprises 100 parts by weight of water, 0.8 part by weight of salicylic acid, 8 parts by weight of aspartic acid and 10 parts by weight of anthocyanin, sowing the test cotton seeds in seedling raising boxes at room temperature, sowing 6 seeds in each box, watering each box with equal amount of water before sowing to ensure that the water content of the soil is consistent, and then not watering any more;
(2) spraying ethanol solution of the composition when cotton seedlings grow to 4-6cm, wherein the concentration of nomilin in the ethanol solution of the composition is 1 mu mol.L-1The concentration of hesperetin is 2.5 mu mol.L-1The composition is continuously sprayed for 3 days, and is sprayed once in the morning and at night, and 14ml of the composition is sprayed to each cotton seedling.
Comparative example 1
This comparative example was conducted in the same manner as in example 2 except that the ethanol solution of the sprayed composition was replaced with the same volume of water in step (2).
Comparative example 2
This comparative example was carried out in the same manner as in example 2 except that no nomilin was added to the ethanol solution of the composition to be sprayed in step (2).
Comparative example 3
This comparative example was conducted in the same manner as in example 2 except that no hesperetin was added to the ethanol solution of the composition to be sprayed in step (2).
Comparative example 4
This comparative example was conducted in the same manner as in example 5 except that the ethanol solution of the composition for root irrigation was replaced with the same volume of water in step (2).
Test example 1
The cotton is treated according to the methods of examples 1-6 and comparative examples 1-4 respectively, 50 boxes of cotton seedlings are planted in each group, 300 cotton seedlings in each group are planted in each group, 50ml of water is poured into each seedling box for rehydration treatment for 24 hours in the fourth day after spraying or root irrigation treatment, the growth condition of new leaves of the cotton seedlings after rehydration treatment for three days is observed, survival is determined when new leaves grow out, and the survival rate of each group is calculated, wherein the results are shown in table 1.
TABLE 1
Figure BDA0002139680810000071
As can be seen from Table 1, the cotton seedlings treated by the methods of examples 1 to 6 all have higher survival rates than those of examples 1 to 4, and the cotton seedlings treated by the methods of examples 4 to 6 have higher survival rates than those of examples 1 to 3, and the cotton seedlings treated by comparative examples 2, 3 and 4 have higher survival rates than those of comparative example 1 and are obviously lower than those of examples 1 to 6, because the drought resistance of the cotton seedlings can be enhanced by seed soaking, spraying or root pouring compositions, and the drought resistance of the cotton seedlings is better by combining the seed soaking, spraying or root pouring compositions, and the survival rates of the cotton seedlings are lower by only spraying nomilin or hesperetin, which shows that the combined survival rates of the two nomilin and hesperetin can enhance the drought resistance of the cotton seedlings.
Test example 2
Investigating the influence of different nomilin and hesperetin molar ratios on the survival rate of cotton seedlings
Cotton seedlings were treated in the same manner as in example 2 except that the molar ratio of nomilin to hesperetin was changed to examine the survival rate, which was calculated in the same manner as in test example 1, and the results are shown in Table 2.
TABLE 2
Figure BDA0002139680810000081
As can be seen from table 2, the molar ratio of nomilin and hesperetin has an effect on the survival rate of cotton seedlings, in the range of 1 μmol: the survival rate was highest at 2. mu. mol.
The inventors have also conducted the above experiments on other examples, and the results are substantially consistent, and are not listed due to space limitation.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (9)

1. A composition for enhancing drought resistance of cotton seedlings, comprising nomilin and hesperetin in a molar ratio of 0.5 to 1.5 μmol: 1-3 mu mol;
the method for enhancing the drought resistance of cotton seedlings by using the composition comprises the following steps:
(1) firstly, selecting test cotton seeds, then sowing and watering;
(2) spraying or irrigating the ethanol solution of the composition for root after cotton seedling grows out, continuously spraying or irrigating the root for 3 days, and spraying or irrigating the root once in the morning and evening.
2. The composition for enhancing drought resistance of cotton seedlings according to claim 1, wherein the molar ratio of nomilin to hesperetin is 1 μmol: 2. mu. mol.
3. The composition for enhancing drought resistance of cotton seedlings according to claim 1, wherein the cotton seeds in the step (1) are further subjected to seed soaking treatment, and the cotton seeds are soaked in the seed soaking liquid for 16-24 h.
4. The composition for enhancing drought resistance of cotton seedlings according to claim 3, wherein the seed soaking solution comprises 100 parts by weight of water, 0.4-0.8 part by weight of salicylic acid, 4-8 parts by weight of aspartic acid and 6-10 parts by weight of anthocyanin.
5. The composition for enhancing drought resistance of cotton seedlings according to claim 4, wherein the seed soaking solution comprises 100 parts by weight of water, 0.6 part by weight of salicylic acid, 6 parts by weight of aspartic acid and 8 parts by weight of anthocyanin.
6. The composition for enhancing drought resistance of cotton seedlings according to claim 1, wherein spraying or irrigating the ethanol solution of the composition is performed when the seedlings grow to 4-6cm in the step (2).
7. A composition for enhancing drought resistance of cotton seedlings according to claim 1 or 6, wherein the concentration of nomilin in the ethanol solution of the composition is 0.5-1.5 μmol-L-1The concentration of hesperetin is 1-3 mu mol.L-1。
8. The composition for enhancing the drought resistance of cotton seedlings according to claim 1, wherein 6-14ml of the ethanol solution of the composition is sprayed or irrigated to the roots of each cotton seedling in the step (2).
9. The composition for enhancing drought resistance of cotton seedlings according to claim 8, wherein 10ml of ethanol solution of the composition is sprayed or irrigated to roots of each cotton seedling in the step (2).
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