CN112080483A - Hemp fiber degumming composite enzyme and preparation method thereof - Google Patents
Hemp fiber degumming composite enzyme and preparation method thereof Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01011—Pectinesterase (3.1.1.11)
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- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01015—Polygalacturonase (3.2.1.15)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02002—Pectate lyase (4.2.2.2)
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
- D01C1/02—Treatment of vegetable material by chemical methods to obtain bast fibres
Abstract
The invention relates to the technical field of textile auxiliary agents, in particular to a hemp fiber degumming composite enzyme and a preparation method thereof. The hemp fiber degumming composite enzyme is prepared from the following components in percentage by weight: xylanase: 6% -9%; cellulase: 2% -5%; and (3) pectinase: 20% -25%; chelating dispersant: 35% -44%; inhibitor (B): 16% -34%; nonionic polymer surfactant: 1% -3%; the preparation method of the hemp fiber degumming compound enzyme comprises the steps of weighing, stirring in stages, heating for reaction, and rotating and outputting the granular hemp fiber degumming compound enzyme by an agglomeration forming machine. The degumming composite enzyme is prepared by a method combining biology and chemistry, the formula combination is optimized, and a good degumming effect is achieved. The invention can control the residual quantity of the hemp fiber, pectin and lignin after degumming in a range suitable for dyeing and finishing, the hemicellulose is completely reserved, the dye uptake rate is high, the fastness is high, the spinnability of spun yarn is high, the yarn yield is high, the surface of the fabric is smooth and clean, and the hand feeling is soft.
Description
Technical Field
The invention relates to the technical field of textile auxiliary agents, in particular to a hemp fiber degumming composite enzyme and a preparation method thereof.
Background
Flax is the first natural plant fiber used by human beings, and has a history of more than 1 ten thousand years. Flax is a pure natural fiber, and is increasingly regarded by human beings due to its remarkable characteristics of sweat absorption, good air permeability, no harm to human bodies and the like. Flax is an important high-quality natural fiber and has many excellent wearability. The flax roving contains a large amount of cellulose accompanying matters such as hemicellulose, lignin, pectin, lipowax and the like besides main components of cellulose. The existence of these cellulose concomitants affects, on the one hand, the spinnability of the flax spun yarn subsequently spun, the quality of the spun yarn and the spun high count yarn, and on the other hand, the physical and mechanical properties, the dyeing properties and the wearability of the spun yarn. Therefore, before spinning the spun yarn, the flax roving needs to be treated, commonly called boiling-off of the flax roving or degumming of the flax fiber.
At present, a plurality of degumming methods for flax, such as a chemical degumming method, a biological enzyme method, a physical method and the like, have advantages and disadvantages. The general chemical degumming method is to utilize the hydrolysis of acid, alkali and oxidant to destroy colloid macromolecules to make the colloid macromolecules become micromolecules which can be dissolved in solution, but the chemical method has high cost and high energy consumption, the generated wastewater is difficult to treat, the pollution to water quality is serious, the fiber damage is serious, the fiber hand feeling is poorer, in the chemical degumming process, part of removed colloid is attached to the surface of the fiber and is difficult to remove by washing, and after the fibrilia is cooled and dried, the part of colloid is re-solidified and adhered to the fiber, thereby greatly influencing the degumming effect of the fiber; the biological enzyme method has the problems of unstable degumming quality, long time consumption and the like, and the treated flax fiber has thick and hard hand feeling, low splitting degree, low coloring property and spinnability and causes difficulty in the processing of the subsequent procedure. The biological-chemical degumming method is the most potential flax degumming method at present, combines the advantages of the first two degumming methods and achieves good degumming effect. However, the degumming enzyme auxiliary agent used in the current biological-chemical degumming method has poor removal effect on the flax particles, the flax scraps and the flax peels remained in the flax fibers, so that the degumming effect is poor, the flax fibers have rough and hard handfeel and low splitting degree, and the processing of the subsequent process is influenced.
Patent CN 107385523A discloses a biological enzyme fibrilia degumming agent, which comprises the following components by volume: 70-80% of complex enzyme, 10-15% of stabilizing agent, 6-10% of penetrating agent and 4-5% of chelating agent; the compound enzyme comprises the following components in parts by weight: 30 parts of hemicellulase, 20 parts of pectinase, 20 parts of lignin degrading enzyme, 15 parts of cellulase, 12 parts of cellobiase and 8 parts of neutral protease. Patent CN 107385523A discloses an enzyme adjuvant used in cotton flax and linen viscose grey cloth cold batch process, which is prepared from the following raw materials by weight percent, desizing enzyme 3-5%; 10-15% of flax degumming enzyme; 0.5-2% of neutral cellulase; 3-5% of high-efficiency penetrant; 73-83.5% of distilled water and a preparation method thereof.
The components of the composite enzyme disclosed by the patent are hemicellulase, pectinase, lignin degrading enzyme, cellulase, cellobiase and neutral protease, the types of enzymes are more during preparation, the preparation process is complex, and the composite enzyme in the degumming agent is 70-80% in volume, and the total volume of the stabilizer, the penetrating agent and the chelating agent is 20-30%, so that the residual quantity of flax fibers, pectin and lignin cannot be controlled within a range suitable for dyeing and finishing after treatment, the retention integrity of hemicellulose cannot be ensured, and the subsequent dyeing rate is low and the dyeing fastness is poor; in addition, potassium sulfate is adopted in the preparation of the stabilizer, so that the discharge of wastewater and sewage is increased, the environment is polluted, and the environmental protection is not facilitated.
Disclosure of Invention
Aiming at the defects in the prior art, the technical problem to be solved by the invention is to provide the complex enzyme for degumming the fibrilia, which can control the residual quantity of the fibrilia, pectin and lignin after degumming in a range suitable for dyeing and finishing, keep the hemicellulose completely, and has high dye uptake, high fastness, high spinnability of spun yarn, high yarn yield, smooth fabric surface and soft hand feeling, and the preparation method thereof.
The technical scheme adopted by the invention for realizing the purpose is as follows: a composite enzyme for degumming hemp fiber is prepared from the following components in percentage by weight:
xylanase: 6% -9%;
cellulase: 2% -5%;
and (3) pectinase: 20% -25%;
chelating dispersant: 35% -44%;
inhibitor (B): 16% -34%;
nonionic polymer surfactant: 1% -3%;
the composite enzyme for degumming the hemp fiber comprises the following specific components in percentage by weight:
xylanase: 6 percent;
cellulase: 2 percent;
and (3) pectinase: 20 percent;
a chelating dispersant; 44%;
inhibitor (B): 27%;
nonionic polymer surfactant: 1 percent;
the composite enzyme for degumming the hemp fiber comprises the following specific components in percentage by weight:
xylanase: 7 percent;
cellulase: 4 percent;
and (3) pectinase: 22 percent;
chelating dispersant: 39 percent;
inhibitor (B): 26 percent;
nonionic polymer surfactant: 2 percent;
the composite enzyme for degumming the hemp fiber comprises the following specific components in percentage by weight:
xylanase: 9 percent;
cellulase: 5 percent;
and (3) pectinase: 25 percent;
chelating dispersant: 35 percent;
inhibitor (B): 23 percent;
nonionic polymer surfactant: 3 percent;
the chelating dispersant is one or more of maleic anhydride (HPMA), polyacrylic acid (PAA) and polyhydroxyacrylic acid mixture.
The inhibitor of the complex enzyme for degumming the hemp fiber is one or two of zinc sulfate and carboxymethyl cellulose mixture.
In the compound enzyme for degumming fibrilia, the nonionic polymer surfactant is fatty alcohol-polyoxyethylene ether.
A method for preparing a composite enzyme for degumming hemp fibers comprises the following steps:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 25-28 ℃, after the temperature is stable, adding 35-44 wt% of chelating dispersant and 1-3 wt% of nonionic polymer surfactant into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 100-200rpm, the stirring time is 10-20min, stirring uniformly, and controlling the temperature of the reaction kettle at 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 6-9 wt% of xylanase, 2-5 wt% of cellulase and 20-25 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150-200rpm, and the stirring time is 20-30min until stirring is uniform;
(4) adding 16-34 wt% of inhibitor, stirring at 150-200rpm for 20-30min until stirring;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
The preparation method of the hemp fiber degumming compound enzyme comprises the following steps:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 26 ℃, after the temperature is stable, adding 39 wt% of chelating dispersant and 2 wt% of nonionic polymer surfactant into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150rpm, the stirring time is 15min, stirring uniformly, and controlling the temperature of the reaction kettle to be 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 7.5 wt% of xylanase, 4 wt% of cellulase and 23 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 170rpm, and the stirring time is 25min until stirring is uniform;
(4) adding inhibitor with weight percentage of 24.5%, stirring at a rotation speed of 180rpm for 24min until stirring uniformly;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
The hemp fiber degumming composite enzyme and the preparation method thereof have the beneficial effects that: the xylanase in the composite hemp fiber degumming enzyme can effectively remove lignin, the cellulase can effectively remove cellulose, and the pectinase can effectively remove pectin, and meanwhile, the compound use of the xylanase, the cellulase and the pectinase improves the removal rate of residual flax particles, flax scraps and flax peels in flax fibers, the degumming effect is good, the surface of a hemp fabric is smooth, and the hand feeling is soft; the addition of the nonionic surfactant reduces the surface tension of water, and a micelle can be formed after the nonionic surfactant reaches a certain concentration, so that the degumming composite enzyme is wetted and then is bonded into blocks, the treated fiber is degummed uniformly, and the surface of the fibrilia fabric is smooth. The degumming composite enzyme contains less chemical components, reduces the discharge amount of waste water and sewage, and is beneficial to environmental protection. The invention controls the residual quantity of the hemp fiber, the pectin and the lignin after the degumming treatment in a range suitable for dyeing and finishing, keeps the hemicellulose completely, and has high dye uptake, high fastness, high spinnability of spun yarn and high yarn yield. The invention has the advantages of little emission pollution, meeting the requirement of environmental protection, high degumming speed, high efficiency, water and energy saving of 30 to 50 percent, little strength damage to the fibrilia fabric and softer hand feeling of the treated fibrilia fabric.
Detailed Description
The present invention will be described in further detail with reference to specific examples;
example 1:
a composite enzyme for degumming hemp fiber is prepared from the following components in percentage by weight:
xylanase: 6 percent;
cellulase: 2 percent;
and (3) pectinase: 20 percent;
a chelating dispersant; 44%;
inhibitor (B): 27%;
nonionic polymer surfactant: 1 percent.
A method for preparing a composite enzyme for degumming hemp fibers comprises the following steps:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 25 ℃, after the temperature is stable, adding 44 weight percent of maleic anhydride (HPMA) and 1 weight percent of fatty alcohol-polyoxyethylene ether into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 100rpm, the stirring time is 10min, and stirring uniformly, wherein the temperature of the reaction kettle is controlled to 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 6 wt% of xylanase, 2 wt% of cellulase and 20 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150rpm, and the stirring time is 20min until stirring is uniform;
(4) adding 27 wt% of zinc sulfate, wherein the rotating speed of a stirring device is 150rpm, and the stirring time is 20min until the mixture is uniformly stirred;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
Example 2:
a composite enzyme for degumming hemp fiber is prepared from the following components in percentage by weight:
xylanase: 7 percent;
cellulase: 4 percent;
and (3) pectinase: 22 percent;
chelating dispersant: 39 percent;
inhibitor (B): 26 percent;
nonionic polymer surfactant: 2 percent.
Example 2:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 26 ℃, after the temperature is stable, adding 39 weight percent of polyacrylic acid (PAA) and 2 weight percent of fatty alcohol-polyoxyethylene ether into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150rpm, the stirring time is 15min, stirring uniformly, and controlling the temperature of the reaction kettle to be 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 7 wt% of xylanase, 4 wt% of cellulase and 22 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 170rpm, and the stirring time is 25min until stirring is uniform;
(4) adding a carboxymethyl cellulose mixture with the weight percentage of 26%, wherein the rotating speed of a stirring device is 180rpm, and the stirring time is 24min until the mixture is uniformly stirred;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
Example 3:
a composite enzyme for degumming hemp fiber is prepared from the following components in percentage by weight:
xylanase: 9 percent;
cellulase: 5 percent;
and (3) pectinase: 25 percent;
chelating dispersant: 35 percent;
inhibitor (B): 23 percent;
nonionic polymer surfactant: 3 percent.
(1) And weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 28 ℃, after the temperature is stable, adding 35 wt% of polyhydroxyacrylic acid mixture and 3 wt% of fatty alcohol-polyoxyethylene ether into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 200rpm, the stirring time is 20min, stirring uniformly, and controlling the temperature of the reaction kettle to be 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 9 wt% of xylanase, 5 wt% of cellulase and 25 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 200rpm, and the stirring time is 30min until stirring is uniform;
(4) adding 23 wt% of carboxymethyl cellulose mixture, stirring at 200rpm for 20-30min until stirring uniformly;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made according to the spirit of the present disclosure should be covered within the scope of the present disclosure.
Claims (9)
1. The composite enzyme for degumming fibrilia is characterized by being prepared from the following components in percentage by weight:
xylanase: 6% -9%;
cellulase: 2% -5%;
and (3) pectinase: 20% -25%;
chelating dispersant: 35% -44%;
inhibitor (B): 16% -34%;
nonionic polymer surfactant: 1 to 3 percent.
2. The composite enzyme for degumming fibrilia according to claim 1, which is characterized by comprising the following specific components in percentage by weight:
xylanase: 6 percent;
cellulase: 2 percent;
and (3) pectinase: 20 percent;
a chelating dispersant; 44%;
inhibitor (B): 27%;
nonionic polymer surfactant: 1 percent.
3. The composite enzyme for degumming fibrilia according to claim 1, which is characterized by comprising the following specific components in percentage by weight:
xylanase: 7 percent;
cellulase: 4 percent;
and (3) pectinase: 22 percent;
chelating dispersant: 39 percent;
inhibitor (B): 26 percent;
nonionic polymer surfactant: 2 percent.
4. The composite enzyme for degumming fibrilia according to claim 1, which is characterized by comprising the following specific components in percentage by weight:
xylanase: 9 percent;
cellulase: 5 percent;
and (3) pectinase: 25 percent;
chelating dispersant: 35 percent;
inhibitor (B): 23 percent;
nonionic polymer surfactant: 3 percent.
5. The hemp fiber degumming complex enzyme according to claim 1, which is characterized in that: the chelating dispersant is one or more of maleic anhydride, polyacrylic acid and polyhydroxyacrylic acid mixture.
6. The hemp fiber degumming complex enzyme according to claim 1, which is characterized in that: the inhibitor is one or two of zinc sulfate and carboxymethyl cellulose mixture.
7. The hemp fiber degumming complex enzyme according to claim 1, which is characterized in that: the nonionic polymer surfactant is fatty alcohol-polyoxyethylene ether.
8. A method for preparing the complex enzyme for degumming hemp fiber as claimed in claims 1-7, which is characterized by comprising the following steps:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 25-28 ℃, after the temperature is stable, adding 35-44 wt% of chelating dispersant and 1-3 wt% of nonionic polymer surfactant into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 100-200rpm, the stirring time is 10-20min, stirring uniformly, and controlling the temperature of the reaction kettle at 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 6-9 wt% of xylanase, 2-5 wt% of cellulase and 20-25 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150-200rpm, and the stirring time is 20-30min until stirring is uniform;
(4) adding 16-34 wt% of inhibitor, stirring at 150-200rpm for 20-30min until stirring;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
9. The preparation method of the hemp fiber degumming complex enzyme according to claim 8, which is characterized by comprising the following steps:
(1) and weighing: inspecting various raw materials according to requirements, and weighing the raw materials according to a formula ratio for later use;
(2) adjusting the temperature of the reaction kettle to 26 ℃, after the temperature is stable, adding 39 wt% of chelating dispersant and 2 wt% of nonionic polymer surfactant into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 150rpm, the stirring time is 15min, stirring uniformly, and controlling the temperature of the reaction kettle to be 25 ℃ in the stirring process;
(3) after stirring, continuously controlling the temperature of the reaction kettle to be 25 ℃, then sequentially adding 7.5 wt% of xylanase, 4 wt% of cellulase and 23 wt% of pectinase into the reaction kettle, starting a stirring device, wherein the rotating speed of the stirring device is 170rpm, and the stirring time is 25min until stirring is uniform;
(4) adding inhibitor with weight percentage of 24.5%, stirring at a rotation speed of 180rpm for 24min until stirring uniformly;
(5) and (4) outputting granular hemp fiber degumming compound enzyme by rotating the agglomeration forming machine.
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