CN112080396B - Stropharia rugoso-annulata liquid strain incubator and culturing method - Google Patents
Stropharia rugoso-annulata liquid strain incubator and culturing method Download PDFInfo
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Abstract
The invention discloses a stropharia rugoso-annulata liquid strain incubator, which is characterized by comprising a liquid stock culture tank (1), a temperature-control magnetic rotary table (2) and a combined stirrer (3), wherein the liquid stock culture tank (1) is arranged above the temperature-control magnetic rotary table (2), stock culture liquid containing liquid stock is loaded in the liquid stock culture tank (1), and the combined stirrer (3) is placed in the stock culture liquid; the rotation of the temperature-control magnetic turntable (2) drives the rotation of the combined stirrer (3) to realize the non-contact stirring and heating of the stock culture solution. When the culture device disclosed by the invention is used for culturing strains, compared with a solid stock seed, the time for culturing the stock seed can be shortened by 40-60 days, and the time for growing the cultivated strain can be shortened by 30-50 days.
Description
Technical Field
The invention belongs to the field of fungus cultivation, and particularly relates to a stropharia rugoso-annulata liquid strain incubator and a cultivation method.
Background
The stropharia rugoso-annulata (Stropharia rugoso-annulata) is also called tricholoma matsutake and Yun Rong, the fungus caps of the stropharia rugoso-annulata are wine red, the meat quality of the stropharia rugoso-annulata is crisp and tender, and the stropharia rugoso-annulata is one of ten stropharia rugoso-annulata in the international mushroom trade market, can consume various agricultural and forestry wastes, has a simple cultivation mode, can adopt raw material open field or facility cultivation, and is suitable for solving the problem of agricultural straw recycling caused by the forbidden burning of straw in China.
At present, the second-level strain (stock strain) of the stropharia rugoso-annulata is divided into a solid strain and a liquid strain, the spawn growth of the solid strain is slow, the difference between the upper activity and the lower activity of the strain is large, the inoculation operation is slow, and the hypha growth speed is inconsistent after the third-level strain is inoculated; the liquid strain manufacturing method generally adopts the technology of a shaking table and a fermentation tank, the efficiency of manufacturing the secondary liquid strain through the shaking table is low, and the fermentation tank has high cost and is not convenient for transportation. The three-stage stropharia rugoso-annulata seed culture time is long, the hypha growth speed is about 2mm/d, the room temperature is controlled to be about 25 ℃ in summer for preparing the spawn, the hypha is filled in bags for about 85 days, and the culture cost is far greater than the material and labor cost.
Disclosure of Invention
Aiming at the problems in the background technology, the invention provides a stropharia rugoso-annulata liquid strain incubator and an incubation method. Can shorten the strain culture time, reduce the cost and rapidly transport liquid strains.
The invention discloses a stropharia rugoso-annulata liquid strain incubator, which comprises a liquid stock culture tank, a temperature-control magnetic turntable and a combined stirrer, wherein the liquid stock culture tank is arranged above the temperature-control magnetic turntable, stock culture liquid containing liquid stock is loaded in the liquid stock culture tank, and the combined stirrer is placed in the stock culture liquid; the rotation of the temperature-control magnetic turntable drives the rotation of the combined stirrer, so that the non-contact stirring and heating of the stock culture solution are realized.
Preferably, the top of the liquid stock culture tank is provided with a strain injection port and a liquid adding port, the strain injection port is provided with a rubber cover to realize the opening and the closing, and the liquid adding port is provided with a rubber plug to realize the opening and the closing; a liquid taking tap is arranged at the bottom of the liquid stock culture tank, and the liquid taking tap is sealed by a sealing film in the preparation process of stock culture liquid; the liquid taking tap is connected with a rubber tube in the inoculation process, the outlet end of the rubber tube is a glass conical opening, glass beads are arranged in the rubber tube, and the diameter of the glass beads is slightly larger than that of the rubber tube; the bottom of the liquid stock culture tank is provided with annular supporting feet; a graduated scale and a handle are arranged on the body of the liquid stock culture tank; the bottom center downside of cultivateing the jar sets up the recess, and the bottom center upside of cultivateing the jar corresponds and sets up the recess, and two recesses are used for spacing stirring's both ends.
Preferably, a magnetic disk is arranged at the top of the temperature-control magnetic turntable, and the magnetic disk is controlled to rotate by a motor; the body of the temperature-control magnetic turntable is provided with a power switch, a temperature adjusting knob, a rotating speed adjusting knob, a display screen and a power line.
Preferably, the stirrer comprises a magnetic rotor and a plastic rotor, and the magnetic rotor and the plastic rotor are connected through a bearing.
Preferably, the plastic rotor is longitudinally movable at an upper portion of the bearing, and the magnetic rotor is fixed at a lower portion of the bearing.
Preferably, the magnetic rotor is a hexagonal cross rotor, the plastic rotor has no edges and corners, and the magnetic rotor is an olive cross rotor.
The invention also discloses a method for culturing the stropharia rugoso-annulata liquid strain, which comprises the following components in parts by weight
Liquid mother culture medium: 4-6 parts of wood dust powder, 4-6 parts of glucose, 0.5-1.5 parts of bean pulp and 986-992 parts of water;
stock culture solution: 9-11 parts of corn flour, 3-5 parts of wood dust powder, 9-11 parts of glucose, 2-4 parts of bean pulp and 969-977 parts of water;
cultivation seed nutrients: 38-42 parts of 3mm mixed wood chips and 38-42 parts of 8mm mixed wood chips; 7-9 parts of chaff, 4-6 parts of bean pulp, 5-7 parts of corn flour, 0.5-1.5 parts of light calcium carbonate and 892-908 parts of water;
the method comprises the following steps:
s1, preparing a liquid mother culture solution, subpackaging the liquid mother culture solution into a plurality of conical flasks, plugging a bottle mouth by a silica gel plug with air holes, placing the bottle mouth into an autoclave, and cooling to room temperature after sterilization; obtaining a liquid mother culture solution;
s2, taking test tube mother seeds stored in a refrigerator, picking hypha blocks by using an inoculating needle, inoculating the hypha blocks into a liquid mother seed culture solution, and standing for 24 hours; placing into a shaking table, rotating at 190rpm, and culturing for 48h; standing for 24 hours; continuously placing the mixture into a shaking table, rotating at 190rpm, and culturing for 192h; obtaining a liquid mother seed;
s3, taking out the cultured liquid mother seeds, crushing hyphae in an ultra-clean workbench by using a refiner, and injecting all the liquid mother seeds into stock culture solution from a rubber plug by using a sterilized needle cylinder, wherein the stock culture solution is loaded in a liquid stock culture tank;
s4, standing the liquid stock culture tank for 12 hours, then placing the liquid stock culture tank on a temperature-control magnetic turntable, starting the temperature-control magnetic turntable, keeping the temperature at 25 ℃ and increasing the rotating speed to enable the liquid in the tank to slowly flow;
s5, preparing a culture material according to a culture material formula, filling the culture material into a polypropylene fungus bag, sealing the bag by using a special lantern ring of edible fungi, inserting a grid inoculation rod into the lantern ring by using a booster cone, covering a lantern ring cover, placing the bag into a sterilizing pot, sterilizing at the temperature of 121 ℃ for 2 hours by adopting high-pressure sterilization, placing the fungus bag into a cooling chamber fumigated and sterilized by using sodium iso-chlorophilic urate after sterilization, and cooling to the room temperature;
s6, taking off a sealing film on a liquid taking tap in an ultra-clean workbench, burning the liquid taking tap on an outer flame of an alcohol lamp, connecting a sterilized rubber tube on the liquid taking tap after the liquid taking tap is cooled, opening a lantern ring cover of a cultivated species, pinching glass beads on the rubber tube, squeezing 5ml-10ml of liquid strain into a grid inoculating rod, enabling the liquid strain to flow to the bottom along the grid inoculating rod, covering the lantern ring cover, and placing a connected fungus bag in a 25 ℃ environment for culturing for 45 days.
Preferably, in S3, the stock culture solution is obtained by:
s3-1, preparing stock culture solution;
s3-2, filling the stock culture solution into a liquid stock culture tank (1) through a liquid filling opening (1-7), plugging the liquid filling opening (1-7) by a silica gel plug (1-8), keeping the liquid stock culture tank (1) in a closed state, and placing the liquid stock culture tank into an autoclave for sterilization at 121 ℃ for 20min;
s3-3, sterilizing the liquid stock culture tank, and then putting the liquid stock culture tank into a sterile environment to be cooled to room temperature.
The beneficial effects of the invention are that
The device is simple, low in cost, light and portable, the culture area is not limited, the rotor in the fermentation bottle is driven by magnetic force to enable the culture solution to rotate at a proper rotation speed, and the method can accelerate the growth speed of the strain, increase the biomass of hyphae and enable the strain to grow to a usable state in a short time. The device can uniformly perform fungus growth in a fungus growth chamber, and is transported to an inoculation place after the fungus growth is completed, and by combining the culture method of the invention, the inoculation can be performed rapidly under simple conditions by using the grid-shaped inoculation rod, the activity of mycelia on and from a fungus bag after inoculation is uniform and consistent, and the bag filling time of mycelia is shortened due to strong ventilation in the bag, so that the culture cost can be greatly reduced.
The application also provides a brand new liquid mother culture solution, stock culture solution, cultivation seed nutrient formula and a corresponding cultivation method. The device is matched with the device of the invention to accelerate the growth speed of the strain to the greatest extent, shorten the mycelium cultivation time and improve the mycelium biomass.
Drawings
FIG. 1 is a schematic diagram showing the structure of a liquid stock culture tank according to the present invention
FIG. 2 is a schematic diagram of a temperature-controlled magnetic turntable according to the present invention
FIG. 3 is a schematic view of a stirrer according to the present invention
FIG. 4 is a schematic view of the booster cone and inoculating rod of the present invention
FIG. 5 is a schematic view showing the use of the inoculating rod of the present invention
Detailed Description
The invention is further illustrated below with reference to examples, but the scope of the invention is not limited thereto:
referring to fig. 1-5, a stropharia rugoso-annulata liquid strain incubator comprises a liquid stock culture tank (1), a temperature-control magnetic rotary table (2) and a combined stirrer (3), wherein the liquid stock culture tank (1) is arranged above the temperature-control magnetic rotary table (2), stock culture solution containing liquid stock is loaded in the liquid stock culture tank (1), and the combined stirrer (3) is placed in the stock culture solution; the rotation of the temperature-control magnetic turntable (2) drives the rotation of the combined stirrer (3) to realize the non-contact stirring and heating of the stock culture solution.
Preferably, the top of the liquid stock culture tank (1) is provided with a strain injection port (1-1) and a liquid adding port (1-7), the strain injection port (1-1) is provided with a rubber cover (1-2) to realize the opening and the closing, and the liquid adding port (1-7) is provided with a rubber plug (1-8) to realize the opening and the closing; the bottom of the liquid stock culture tank (1) is provided with a liquid taking tap (1-3), and the liquid taking tap (1-3) is sealed by a sealing film in the preparation process of stock culture liquid; the liquid taking tap (1-3) is connected with a rubber tube (1-4) in the inoculation process, the outlet end of the rubber tube (1-4) is a glass conical opening (1-5), a glass bead (1-6) is arranged in the rubber tube (1-4), and the diameter of the glass bead (1-6) is slightly larger than the diameter of the rubber tube (1-4); the bottom of the liquid stock culture tank (1) is provided with annular supporting feet (1-9); a graduated scale (1-10) and a handle (1-11) are arranged on the body of the liquid stock culture tank (1); the upper side of the center of the top of the culture tank (1) is provided with a groove, the upper side of the center of the bottom of the culture tank (1) is correspondingly provided with a groove, and the two grooves are used for limiting the two ends of the stirrer (3).
Preferably, a magnetic disk (2-1) is arranged at the top of the temperature control magnetic turntable (2), and the magnetic disk (2-1) is controlled to rotate by a motor; the body of the temperature-control magnetic turntable (2) is provided with a power switch (2-2), a temperature adjusting knob (2-3), a rotating speed adjusting knob (2-4), a display screen (2-5) and a power line (2-6).
Preferably, the stirrer (3) comprises a magnetic rotor (3-1) and a plastic rotor (3-2), and the magnetic rotor (3-1) and the plastic rotor (3-2) are connected through a bearing (3-3).
Preferably, the plastic rotor (3-2) is longitudinally movable at the upper part of the bearing (3-3), and the magnetic rotor (3-1) is fixed at the lower part of the bearing (3-3).
Preferably, the magnetic rotor (3-1) is a hexagonal cross rotor, and the plastic rotor (3-2) has no edges and corners and is an olive cross rotor.
In the following, it will be described how to use the incubator of the present invention to culture a liquid strain of stropharia rugoso-annulata, with reference to specific examples, the specific dimensions of the incubator used in the examples are as follows:
150ml of conical flask; the diameter of the liquid stock culture tank (1) is 300mm, the height is 450mm, and the total volume is 30L; the width of the annular support leg (1-9) is 9cm, and the height is 3cm; the width of the liquid adding opening (1-7) is 8cm; the width of the strain injection opening (1-1) is 26mm; the external diameter of the temperature-control magnetic turntable (2) is 30cm, and the total height is 120mm; the diameter of the magnetic disc (2-1) is 100mm, and the height is 25mm; the controllable range of the rotating speed is 0-500 rpm, and the controllable range of the temperature is 0-35 ℃; a polypropylene fungus bag (4-1) with 15mm multiplied by 28mm; a collar (4-2) special for edible fungi with the diameter of 35 mm; the length of the grid inoculation rod (4-5) is 150mm, the outer diameter is 21mm, the inner diameter is 20mm, and the outside is smooth; the booster cone (4-3) is 350mm long and 19.5mm in outer diameter.
Example 1: according to parts by weight
Liquid mother culture medium: 4 parts of wood dust powder, 4 parts of glucose, 0.5 part of bean pulp and 992 parts of water;
stock culture solution: 9 parts of corn flour, 3 parts of wood dust powder, 9 parts of glucose, 2 parts of bean pulp and 977 parts of water;
cultivation seed nutrients: 38 parts of 3mm mixed wood chips and 38 parts of 8mm mixed wood chips; 7 parts of chaff, 4 parts of bean pulp, 5 parts of corn flour, 0.5 part of light calcium carbonate and 908 parts of water.
Example 2: according to parts by weight
Liquid mother culture medium: 6 parts of wood dust powder, 6 parts of glucose, 1.5 parts of soybean meal and 986 parts of water;
stock culture solution: 11 parts of corn flour, 5 parts of wood dust powder, 11 parts of glucose, 4 parts of bean pulp and 969 parts of water;
cultivation seed nutrients: 42 parts of 3mm miscellaneous wood chips and 42 parts of 8mm miscellaneous wood chips; 9 parts of chaff, 6 parts of bean pulp, 7 parts of corn meal, 1.5 parts of light calcium carbonate and 892 parts of water.
Example 3: according to parts by weight
Liquid mother culture medium: 5 parts of wood dust powder, 5 parts of glucose, 1 part of bean pulp and 990 parts of water;
stock culture solution: 10 parts of corn flour, 4 parts of wood dust powder, 10 parts of glucose, 3 parts of bean pulp and 972 parts of water;
cultivation seed nutrients: 40 parts of 3mm mixed wood chips and 40 parts of 8mm mixed wood chips; 8 parts of chaff, 5 parts of bean pulp, 6 parts of corn flour, 1 part of light calcium carbonate and 900 parts of water.
Based on the formulation in the above 3 examples, the cultivation of stropharia rugoso-annulata liquid strain was performed according to the following steps:
s1, preparing a liquid mother culture solution, subpackaging the liquid mother culture solution into a plurality of conical flasks, filling 70ml of culture solution into each flask, plugging a bottle mouth by a silica gel plug with an air hole, placing the flask into an autoclave, and cooling to room temperature after sterilization; obtaining a liquid mother culture solution; s2, taking test tube mother seeds stored in a refrigerator, picking hypha blocks by using an inoculating needle, inoculating the hypha blocks into a liquid mother seed culture solution, and standing for 24 hours; placing into a shaking table, rotating at 190rpm, and culturing for 48h; standing for 24 hours; continuously placing the mixture into a shaking table, rotating at 190rpm, and culturing for 192h; obtaining a liquid mother seed;
s3, taking out the cultured liquid mother seeds, crushing hyphae in an ultra-clean workbench by using a refiner, and then injecting all the liquid mother seeds into stock culture solution from a rubber plug by using a sterilized needle cylinder, wherein the stock culture solution is loaded into a liquid stock culture tank (1) by using the rubber plug (1-2) in an injection manner;
s4, standing the liquid stock culture tank (1) for 12 hours, then placing the liquid stock culture tank on a temperature-control magnetic rotary table (2), starting the temperature-control magnetic rotary table (2), keeping the temperature at 25 ℃ and increasing the rotating speed to enable the liquid in the tank to slowly flow;
s5, preparing a culture material according to a culture material formula, filling the culture material into a polypropylene fungus bag (4-1), sealing the bag by using a special collar (4-2) of edible fungi, inserting a grid inoculation rod (4-5) into the collar (4-2) by using a booster cone (4-3), covering a collar cover (4-6), placing the bag into a sterilizing pot, sterilizing at the temperature of 121 ℃ for 2 hours by adopting high-pressure sterilization, and placing the fungus bag into a cooling chamber fumigated and sterilized by using sodium iso-chlorodiphenoxylate after sterilization, and cooling to the room temperature;
s6, taking off a sealing film on the liquid taking tap (1-3) in an ultra-clean workbench, burning the liquid taking tap (1-3) on an outer flame of an alcohol lamp, after the liquid taking tap (1-3) is cooled, connecting a sterilized rubber tube (1-4) on the liquid taking tap (1-3), opening a sleeve ring cover (4-6) of a cultivated species, pinching glass beads (1-6) on the rubber tube (1-4), extruding 5ml-10ml of liquid strain into a grid inoculation rod (4-5), enabling the liquid strain to flow to the bottom along the grid inoculation rod (4-5), covering the sleeve ring cover (4-6), and placing the connected fungus bag in a 25 ℃ environment for culturing for 45 days.
In the step S3, the stock culture solution is obtained by the following steps:
s3-1, preparing stock culture solution;
s3-2, filling stock culture solution into a liquid stock culture tank (1) through a liquid filling opening (1-7), filling 15L of culture solution into each tank, plugging the liquid filling opening (1-7) by a silica gel plug (1-8), keeping the liquid stock culture tank (1) in a closed state, and placing the liquid stock culture tank into an autoclave for sterilization at 121 ℃ for 20min;
s3-3, sterilizing the liquid stock culture tank (1), and then putting the liquid stock culture tank into a sterile environment to be cooled to room temperature.
The actual operation is carried out according to the three embodiments, and the obtained strain properties and the cultivation time are as follows:
the formula and the device disclosed by the application are adopted for mycelium culture, so that the strain culture time can be obviously shortened, and the cost is reduced.
The specific embodiments described herein are offered by way of example only to illustrate the spirit of the invention. Those skilled in the art may make various modifications or additions to the described embodiments or substitutions thereof without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.
Claims (7)
1. The stropharia rugoso-annulata liquid strain incubator is characterized by comprising a liquid stock culture tank (1), a temperature-control magnetic rotary table (2) and a combined stirrer (3), wherein the liquid stock culture tank (1) is arranged above the temperature-control magnetic rotary table (2), stock culture liquid containing liquid stock seeds is loaded in the liquid stock culture tank (1), and the combined stirrer (3) is placed in the stock culture liquid; the rotation of the temperature-control magnetic turntable (2) drives the rotation of the combined stirrer (3) to realize the non-contact stirring and heating of the stock culture solution; a strain injection port (1-1) and a liquid adding port (1-7) are arranged at the top of the liquid stock culture tank (1), a rubber cover (1-2) is arranged on the strain injection port (1-1) to realize the opening and the closing, and a rubber plug (1-8) is arranged on the liquid adding port (1-7) to realize the opening and the closing; the bottom of the liquid stock culture tank (1) is provided with a liquid taking tap (1-3), and the liquid taking tap (1-3) is sealed by a sealing film in the preparation process of stock culture liquid; the liquid taking tap (1-3) is connected with a rubber tube (1-4) in the inoculation process, the outlet end of the rubber tube (1-4) is a glass conical opening (1-5), a glass bead (1-6) is arranged in the rubber tube (1-4), and the diameter of the glass bead (1-6) is slightly larger than the diameter of the rubber tube (1-4); the bottom of the liquid stock culture tank (1) is provided with annular supporting feet (1-9); a graduated scale (1-10) and a handle (1-11) are arranged on the body of the liquid stock culture tank (1); the upper side of the center of the top of the culture tank (1) is provided with a groove, the upper side of the center of the bottom of the culture tank (1) is correspondingly provided with a groove, and the two grooves are used for limiting the two ends of the stirrer (3).
2. The stropharia rugoso-annulata liquid strain incubator according to claim 1, characterized in that a magnetic disk (2-1) is arranged at the top of the temperature-control magnetic turntable (2), and the magnetic disk (2-1) is controlled to rotate by a motor; the body of the temperature-control magnetic turntable (2) is provided with a power switch (2-2), a temperature adjusting knob (2-3), a rotating speed adjusting knob (2-4), a display screen (2-5) and a power line (2-6).
3. The stropharia rugoso-annulata liquid strain incubator according to claim 1, characterized in that the stirrer (3) comprises a magnetic rotor (3-1) and a plastic rotor (3-2), and the magnetic rotor (3-1) and the plastic rotor (3-2) are connected through a bearing (3-3).
4. A stropharia rugoso-annulata liquid spawn-incubator according to claim 3, characterized in that the plastic rotor (3-2) is longitudinally movable in the upper part of the bearing (3-3), and the magnetic rotor (3-1) is fixed in the lower part of the bearing (3-3).
5. A stropharia rugoso-annulata liquid strain incubator as claimed in claim 3, characterised in that the magnetic rotor (3-1) is a hexagonal cross rotor, the plastic rotor (3-2) is a olive cross rotor without corners.
6. A method for culturing liquid strain of stropharia rugoso-annulata is characterized by comprising the following steps of
Liquid mother culture medium: 4-6 parts of wood dust powder, 4-6 parts of glucose, 0.5-1.5 parts of bean pulp and 986-992 parts of water;
stock culture solution: 9-11 parts of corn flour, 3-5 parts of wood dust powder, 9-11 parts of glucose, 2-4 parts of bean pulp and 969-977 parts of water;
cultivation seed nutrients: 38-42 parts of 3mm mixed wood chips and 38-42 parts of 8mm mixed wood chips; 7-9 parts of chaff, 4-6 parts of bean pulp, 5-7 parts of corn flour, 0.5-1.5 parts of light calcium carbonate and 892-908 parts of water;
the method comprises the following steps:
s1, preparing a liquid mother culture solution, subpackaging the liquid mother culture solution into a plurality of conical flasks, plugging a bottle mouth by a silica gel plug with air holes, placing the bottle mouth into an autoclave, and cooling to room temperature after sterilization; obtaining a liquid mother culture solution;
s2, taking test tube mother seeds stored in a refrigerator, picking hypha blocks by using an inoculating needle, inoculating the hypha blocks into a liquid mother seed culture solution, and standing for 24 hours; placing into a shaking table, rotating at 190rpm, and culturing for 48h; standing for 24 hours; continuously placing the mixture into a shaking table, rotating at 190rpm, and culturing for 192h; obtaining a liquid mother seed;
s3, taking out the cultured liquid mother seeds, crushing hyphae in an ultra-clean workbench by using a refiner, and injecting all the liquid mother seeds into stock culture solution from a rubber plug by using a sterilized needle cylinder, wherein the stock culture solution is loaded in a liquid stock culture tank (1);
s4, standing the liquid stock culture tank (1) for 12 hours, then placing the liquid stock culture tank on a temperature-control magnetic rotary table (2), starting the temperature-control magnetic rotary table (2), keeping the temperature at 25 ℃ and increasing the rotating speed to enable the liquid in the tank to slowly flow;
s5, preparing a culture material according to a culture material formula, filling the culture material into a polypropylene fungus bag (4-1), sealing the bag by using a special collar (4-2) of edible fungi, inserting a grid inoculation rod (4-5) into the collar (4-2) by using a booster cone (4-3), covering a collar cover (4-6), placing the bag into a sterilizing pot, sterilizing at the temperature of 121 ℃ for 2 hours by adopting high-pressure sterilization, and placing the fungus bag into a cooling chamber fumigated and sterilized by using sodium iso-chlorodiphenoxylate after sterilization, and cooling to the room temperature;
s6, taking off a sealing film on the liquid taking tap (1-3) in an ultra-clean workbench, burning the liquid taking tap (1-3) on an outer flame of an alcohol lamp, after the liquid taking tap (1-3) is cooled, connecting a sterilized rubber tube (1-4) on the liquid taking tap (1-3), opening a sleeve ring cover (4-6) of a cultivated species, pinching glass beads (1-6) on the rubber tube (1-4), extruding 5ml-10ml of liquid strain into a grid inoculation rod (4-5), enabling the liquid strain to flow to the bottom along the grid inoculation rod (4-5), covering the sleeve ring cover (4-6), and placing the connected fungus bag in a 25 ℃ environment for culturing for 45 days.
7. The method for culturing a liquid strain of stropharia rugoso-annulata according to claim 6, wherein in S3, the stock culture solution is obtained by:
s3-1, preparing stock culture solution;
s3-2, filling the stock culture solution into a liquid stock culture tank (1) through a liquid filling opening (1-7), plugging the liquid filling opening (1-7) by a silica gel plug (1-8), keeping the liquid stock culture tank (1) in a closed state, and placing the liquid stock culture tank into an autoclave for sterilization at 121 ℃ for 20min;
s3-3, sterilizing the liquid stock culture tank (1), and then putting the liquid stock culture tank into a sterile environment to be cooled to room temperature.
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| CN1440637A (en) * | 2003-04-01 | 2003-09-10 | 刘永昶 | Liquid edible fungus strain producing method and apparatus |
| CN101790936A (en) * | 2009-02-02 | 2010-08-04 | 春山敏昭 | Liquid fungus culturing apparatus and liquid fungus culturing method |
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| CN1440637A (en) * | 2003-04-01 | 2003-09-10 | 刘永昶 | Liquid edible fungus strain producing method and apparatus |
| CN101790936A (en) * | 2009-02-02 | 2010-08-04 | 春山敏昭 | Liquid fungus culturing apparatus and liquid fungus culturing method |
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