CN106269000A - One can sterilizing thermostat water bath and application - Google Patents
One can sterilizing thermostat water bath and application Download PDFInfo
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- CN106269000A CN106269000A CN201510264436.6A CN201510264436A CN106269000A CN 106269000 A CN106269000 A CN 106269000A CN 201510264436 A CN201510264436 A CN 201510264436A CN 106269000 A CN106269000 A CN 106269000A
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- pot
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- water bath
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Abstract
The invention discloses one can sterilizing thermostat water bath and application, specifically, the method of a kind of cell recovery of disclosure of the invention, described method include the cryopreservation tube that will be equipped with treating recovery cell put into can the step melted of sterilizing thermostat water bath, described can include pot cover, pot, base, built-in temperature electrode, add hot plate, magnetic stirring apparatus, temperature mixing control panel, temperature control equipment and magnetic agitation motor by sterilizing thermostat water bath.The present invention can sterilizing thermostat water bath compared with traditional water bath pot, can be by the taking and placing at any time of rustless steel pot, it is not only convenient for the cleaning of internal wall pot body, and by the water high temperature sterilize in water-bath by the way of moist heat sterilization, the pollution of water in minimizing dust proof workshop thermostat water bath, employing adds hot plate and coordinates agitator, makes heats fast and uniformly, has the advantage that simple in construction, floor space are little, easy to use.
Description
Technical field
The present invention relates to biological technical field, particularly to a kind of thermostat water bath for cell recovery and application.
Background technology
Thermostat water bath be widely used in be dried concentrates, distill, impregnate chemical reagent, dipping medicine and biology make
Agent it can also be used to constant temperature water bath heating and other temperature tests, be biology, heredity, virus, Aquatic product, environmental protection,
The indispensable instrument of medicine, health, biochemical laboratory, analysis room and education and scientific research.Thermostat water bath surface is general
Using electrostatic painting process, inner bag, upper cover all use corrosion resistant plate to make, anticorrosive, use digital display temperature control,
Temperature-controlled precision is high, stable performance.
Traditional thermostat water bath is to use in common laboratory, but requires higher dust proof workshop at laminar flow
The traditional thermostat water bath of interior use has the disadvantage that: the water in water-bath unlimited condition and suitable at a temperature of
Easy long dirt and breed bacteria, generally we can use preservative, such as copper sulfate, sodium azide etc. to process, but
These material poisonous and harmfuls, and perishable rustless steel inwall, and growing of microorganism can not be avoided completely.?
In the cell recovery stage, need the cryopreservation tube fast melt that will have just taken out from liquid nitrogen container, owing to cryopreservation tube can be indirectly
Or directly contact with the water in water-bath, easily cause pollution, therefore the microorganism in the water of water-bath is to cell
Recovery also exists bigger risk.Therefore, it is necessary to provide one can sterilizing thermostat water bath and application with solve
Certainly the problems referred to above.
Summary of the invention
In order to solve the problems referred to above, present inventor provides a kind of method of cell recovery and the one can sterilizing
Thermostat water bath, after adding appropriate purified water in advance, puts into moist heat sterilization cabinet before using in stainless-steel pan body
Middle sterilizing, and under air-proof condition, carry out constant temperature, make water be in the clean environment of relative insulation, it is ensured that
Cleanliness factor before uncapping, after using, changes water sterilizing again, treats that next time, cell recovery used, effectively reduces in water
Microorganism is on cell recovery and the impact of clean room environment.
Therefore, a kind of method that first purpose of the present invention is to provide cell recovery.
Second object of the present invention is that offer one can sterilizing thermostat water bath.
For achieving the above object, the present invention is by the following technical solutions:
According to the first aspect of the invention, it is provided that a kind of method of cell recovery, including will be equipped with waiting to recover
The cryopreservation tube of cell put into can the step melted of sterilizing thermostat water bath, described can include by sterilizing thermostat water bath
Pot cover, pot, base, built-in temperature electrode, add hot plate, magnetic stirring apparatus, temperature mixing control panel,
Temperature control equipment and magnetic agitation motor, described pot cover is provided with air filter, described magnetic stirring apparatus
Being arranged in described pot, one end of described built-in temperature electrode is arranged in described pot, and the other end is with described
Temperature control equipment removably circuit connects, described in add hot plate and be arranged on described base, described temperature controls
Device and described magnetic agitation motor are integrally disposed in described base, described temperature mixing control panel respectively with
Described temperature control equipment and described magnetic agitation motor circuit connect.
Will be equipped with treating that the cryopreservation tube of recovery cell is put into described in it is also preferred that the left can the thawing of sterilizing thermostat water bath include
Following steps:
A, in described pot, add appropriate purified water, described pot cover is covered in described pot；
B, described pot and described pot cover are carried out high temperature sterilize；
Described pot and described pot cover are taken out in C, sterilizing after terminating, be placed in described in add in hot plate, by described built-in
Temperature electrode and described temperature control equipment circuit connect；
D, open described temperature mixing control panel, set bath temperature and mixing speed；
E, treat described pot in bath temperature control in the range of design temperature, open described pot cover, will be equipped with
Treat that the cryopreservation tube of recovery cell is put into described pot and melted.
In an instantiation, the profile of described air filter is disk like filter, and interface is that trapezoidal flexible pipe connects
Mouthful, it being connected with using silica gel hose between described pot cover, shell sizes is about 62mm, and inner membrance uses hydrophobic
PTFE film is as air filtration media, and the aperture of described PTFE film is 0.2 μm, can reuse by autoclaving,
The gas exchange inside and outside water-bath it is used for, it is ensured that the pressure inside and outside water-bath keeps balance in high-pressure sterilizing course.
It is also preferred that the left between described pot cover and described pot use rubber seal rings for seal, avoid after high temperature sterilize with
External microbe contacts.
It is also preferred that the left the both sides external of described pot is provided with handle, it is simple to taking and placing, and the cleaning being prone in pot.
It is also preferred that the left described temperature mixing control panel is arranged at the outside of described base.
It is also preferred that the left the material of described pot is rustless steel.
According to the second aspect of the invention, it is provided that one can sterilizing thermostat water bath, including pot cover, pot,
Base, built-in temperature electrode, add hot plate, magnetic stirring apparatus, temperature mixing control panel, temperature control equipment
With magnetic agitation motor, described pot cover being provided with air filter, described magnetic stirring apparatus is arranged at described pot
Internal, one end of described built-in temperature electrode is arranged in described pot, the other end and described temperature control equipment
Removably circuit connects, described in add hot plate and be arranged on described base, described temperature control equipment and described magnetic
Power stirring motor is integrally disposed in described base, and described temperature mixing control panel controls with described temperature respectively
Device and described magnetic agitation motor circuit connect.
It is also preferred that the left use rubber seal rings for seal between described pot cover and described pot.
It is also preferred that the left the both sides external of described pot is provided with handle.
Beneficial effects of the present invention:
1, rustless steel pot can taking and placing at any time, use before in pot, add appropriate purified water, cover pot cover
Put into and moist heat sterilization cabinet carries out sterilizing, effectively prevent the microorganism impact on environment in water.Sterilized pot
Being placed on temperature and add in hot plate, set bath temperature and mixing speed by control panel, kettle temperature is by adding
The heating of hot plate and pot natural heat dissipation realize thermostatic control, and stirring makes temperature mixing uniform, pot seal
Good.Before using, open pot cover, cryopreservation tube be directly placed in water-bath, rev up, compare either manual resuscitation,
Have a clear superiority at aspects such as ease-to-operate, time, physical demands, pollution rates.
2, solve the problem that water is polluter in thermostat water bath in dust proof workshop, thus effectively reduce water
The middle microorganism impact on environment.The present invention can be applicable to dust proof workshop by sterilizing thermostat water bath, can effectively control
Temperature, constant temperature, while reduction water-bath is on the impact of clean environment most possibly, the safety to cell recovery
Property provides the highest guarantee.
Accompanying drawing explanation
Fig. 1 be the present invention can the front view of sterilizing thermostat water bath.
Fig. 2 be the present invention can the top view of sterilizing thermostat water bath.
Description of reference numerals: 1-air filter；2-handle；3-built-in temperature electrode；4-adds hot plate；5-pot
Lid；6-pot；7-magnetic stirring apparatus；8-temperature mixing control panel；9-base.
Detailed description of the invention
The present invention is described in detail with detailed description of the invention below in conjunction with the accompanying drawings.
As depicted in figs. 1 and 2, the present invention can sterilizing thermostat water bath, including pot cover 5, pot 6, base 9,
Built-in temperature electrode 3, add hot plate 4, magnetic stirring apparatus 7, temperature mixing control panel 8, temperature control equipment and
Magnetic agitation motor.
Air filter 1 it is provided with, the displacement of gas during high-temperature heat sterilization on pot cover 5.Pot cover 5
And use rubber seal rings for seal between pot 6, avoid after high temperature sterilize contacting with external microbe.Pot 6
Both sides external be provided with handle 2, it is simple to taking and placing, and the cleaning being prone in pot.The material of pot 6 is preferably
Rustless steel.Magnetic stirring apparatus 7 is arranged in pot 6, is used for stirring.One end of built-in temperature electrode 3 is arranged at
In pot 6, the other end is connected with temperature control equipment circuit, is used for measuring bath temperature.Add hot plate 4 to be arranged at
On base 9, it is used for placing pot 6.Temperature mixing control panel 8 respectively with temperature control equipment and magnetic agitation
Motor circuit connects, and is used for setting bath temperature and mixing speed.Preferably, temperature mixing control panel 8 sets
It is placed in the outside of base 9.Temperature control equipment and magnetic agitation motor are integrally disposed in base 9, design temperature
After, regulate bath temperature by temperature control equipment, simultaneously by magnetic agitation motor-driven magnetic power agitator 7
Rotating, make the water mix homogeneously in pot 6, heats is good.
Preferably, air filter 1 profile is disk like filter, and interface is trapezoidal hose connection, with pot cover 5
Between use silica gel hose to connect, shell sizes is about 62mm, and inner membrance uses hydrophobic PTFE film as air
Filter medium, aperture is 0.2 μm, can reuse by autoclaving, for water-bath in high-pressure sterilizing course
Inside and outside gas exchange, it is ensured that the pressure inside and outside water-bath keeps balance.
The present invention can comprise the following steps for the method for cell recovery by sterilizing thermostat water bath:
1, first pot cover 5 is opened, use handle 2 pot 6 to be picked up, use purified water by clear for pot 6 inwall
After wash clean, add appropriate purified water, the pot cover 5 installing air filter 1 covered in pot 6,
Make the rubber seal rings for seal between pot cover 5 and pot 6 good；
2, the above-mentioned pot 6 installing purified water and pot cover 5 are positioned over high temperature sterilize in moist heat sterilization cabinet；
3, take out pot 6 and pot cover 5 after sterilizing terminates, the most pot cover 5 do not opened, it is to avoid with extraneous contact,
Utilize air filter 1 substitution gas, after temperature of boiler slightly reduces, be placed in and add in hot plate 4, by built-in temperature
Electrode 3 and temperature control equipment circuit connect；
4, opening temperature mixing control panel 8, set bath temperature and mixing speed, the temperature in pot 6 is led to
Cross and add the heating of hot plate 4 and pot 6 natural heat dissipation to realize thermostatic control, coordinate magnetic stirring apparatus 7, make heating
Constant temperature effect is fast and uniform；
5, in treating pot 6, bath temperature controls in the range of design temperature, opens pot cover 5, will be just from liquid nitrogen container
Middle taking-up put into pot fast melt, now cryopreservation tube outer wall and water-bath equipped with the cryopreservation tube treating recovery cell
The pollution that in sterilized water contact in pot, effectively control water-bath, in water, cell recovery link is brought by microorganism
Risk, coordinates stirring simultaneously, revs up, and compares either manual resuscitation, easy and simple to handle, save the time, save body
Power consumes, reduces pollution rate, it is most important that can guarantee that the concordance of each recovery cell manipulation, meets day by day
Strict GMP requirement；
6, after water-bath uses and terminates, the water in pot 6 can be discarded, degreasing the pan 6, treat cell next time again
Use according to the method described above before Soviet Union.
Claims (10)
1. the method for a cell recovery, it is characterised in that include that the cryopreservation tube that will be equipped with treating recovery cell is put
Enter the step can melted in sterilizing thermostat water bath, described can include pot cover, pot, the end by sterilizing thermostat water bath
Seat, built-in temperature electrode, add hot plate, magnetic stirring apparatus, temperature mixing control panel, temperature control equipment and
Magnetic agitation motor, described pot cover is provided with air filter, and described magnetic stirring apparatus is arranged at described pot
In, one end of described built-in temperature electrode is arranged in described pot, and the other end and described temperature control equipment can
Releasably circuit connects, described in add hot plate and be arranged on described base, described temperature control equipment and described magnetic force
Stirring motor is integrally disposed in described base, and described temperature mixing control panel controls dress with described temperature respectively
Put and connect with described magnetic agitation motor circuit.
2. the method for cell recovery as claimed in claim 1, it is characterised in that described in will be equipped with treating that recovery is thin
The cryopreservation tube of born of the same parents is put into and can the thawing of sterilizing thermostat water bath be comprised the following steps:
A, in described pot, add appropriate purified water, described pot cover is covered in described pot；
B, described pot and described pot cover are carried out high temperature sterilize；
Described pot and described pot cover are taken out in C, sterilizing after terminating, be placed in described in add in hot plate, by described built-in
Temperature electrode and described temperature control equipment circuit connect；
D, open described temperature mixing control panel, set bath temperature and mixing speed；
E, treat described pot in bath temperature control in the range of design temperature, open described pot cover, will be equipped with
Treat that the cryopreservation tube of recovery cell is put into described pot and melted.
3. the method for cell recovery as claimed in claim 1 or 2, it is characterised in that described air filter
Profile be disk like filter, the interface of described air filter is between trapezoidal hose connection, and described pot cover
Employing silica gel hose connects, and the inner membrance of described air filter uses hydrophobic PTFE film to be situated between as air filtration
Matter.
4. the method for cell recovery as claimed in claim 1 or 2, it is characterised in that described pot cover is with described
Rubber seal rings for seal is used between pot.
5. the method for cell recovery as claimed in claim 1 or 2, it is characterised in that the outside of described pot
Both sides are provided with handle.
6. the method for cell recovery as claimed in claim 1 or 2, it is characterised in that described temperature stirring control
Panel processed is arranged at the outside of described base.
7. the method for cell recovery as claimed in claim 1 or 2, it is characterised in that the material of described pot
For rustless steel.
8. can a sterilizing thermostat water bath, including pot cover, pot, base, it is characterised in that also include
Built-in temperature electrode, add hot plate, magnetic stirring apparatus, temperature mixing control panel, temperature control equipment and magnetic force
Stirring motor, described pot cover is provided with air filter, and described magnetic stirring apparatus is arranged in described pot,
One end of described built-in temperature electrode is arranged in described pot, and the other end is detachable with described temperature control equipment
Ground circuit connects, described in add hot plate and be arranged on described base, described temperature control equipment and described magnetic agitation
Motor is integrally disposed in described base, described temperature mixing control panel respectively with described temperature control equipment and
Described magnetic agitation motor circuit connects.
The most as claimed in claim 8 can sterilizing thermostat water bath, it is characterised in that described pot cover and described pot
Rubber seal rings for seal is used between body.
The most as claimed in claim 8 can sterilizing thermostat water bath, it is characterised in that the outside of described pot
Both sides are provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510264436.6A CN106269000A (en) | 2015-05-21 | 2015-05-21 | One can sterilizing thermostat water bath and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510264436.6A CN106269000A (en) | 2015-05-21 | 2015-05-21 | One can sterilizing thermostat water bath and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106269000A true CN106269000A (en) | 2017-01-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510264436.6A Pending CN106269000A (en) | 2015-05-21 | 2015-05-21 | One can sterilizing thermostat water bath and application |
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| Country | Link |
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| CN (1) | CN106269000A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732012A (en) * | 2017-03-24 | 2017-05-31 | 上海涵欧制药设备有限公司 | A kind of pharmaceutical purpose high efficient mixer |
| CN106893666A (en) * | 2017-04-24 | 2017-06-27 | 何向锋 | A kind of multi-functional biomedical instrument |
| CN108355729A (en) * | 2018-03-27 | 2018-08-03 | 温州医科大学附属第医院 | Portable water-bath |
| CN112080396A (en) * | 2020-09-22 | 2020-12-15 | 嘉兴市农业科学研究院 | Stropharia rugosoannulata liquid strain incubator and culture method |
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| CN2820312Y (en) * | 2005-09-20 | 2006-09-27 | 南昌大学 | Integrated oil bath electromagnetic stirring heater |
| CN202725211U (en) * | 2012-04-25 | 2013-02-13 | 昆明理工大学 | Constant-temperature water bath pot |
| CN103651331A (en) * | 2013-12-13 | 2014-03-26 | 青岛中天干细胞工程有限公司 | Construction method of pool of adipose-derived stem cells |
| CN104162460A (en) * | 2014-08-21 | 2014-11-26 | 河南科技大学 | Electromagnetic stirring type ultrasonic water bath device |
| CN204724194U (en) * | 2015-05-21 | 2015-10-28 | 上海中信国健药业股份有限公司 | One can sterilizing thermostat water bath |
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2015
- 2015-05-21 CN CN201510264436.6A patent/CN106269000A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2820312Y (en) * | 2005-09-20 | 2006-09-27 | 南昌大学 | Integrated oil bath electromagnetic stirring heater |
| CN202725211U (en) * | 2012-04-25 | 2013-02-13 | 昆明理工大学 | Constant-temperature water bath pot |
| CN103651331A (en) * | 2013-12-13 | 2014-03-26 | 青岛中天干细胞工程有限公司 | Construction method of pool of adipose-derived stem cells |
| CN104162460A (en) * | 2014-08-21 | 2014-11-26 | 河南科技大学 | Electromagnetic stirring type ultrasonic water bath device |
| CN204724194U (en) * | 2015-05-21 | 2015-10-28 | 上海中信国健药业股份有限公司 | One can sterilizing thermostat water bath |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732012A (en) * | 2017-03-24 | 2017-05-31 | 上海涵欧制药设备有限公司 | A kind of pharmaceutical purpose high efficient mixer |
| CN106893666A (en) * | 2017-04-24 | 2017-06-27 | 何向锋 | A kind of multi-functional biomedical instrument |
| CN108355729A (en) * | 2018-03-27 | 2018-08-03 | 温州医科大学附属第医院 | Portable water-bath |
| CN112080396A (en) * | 2020-09-22 | 2020-12-15 | 嘉兴市农业科学研究院 | Stropharia rugosoannulata liquid strain incubator and culture method |
| CN112080396B (en) * | 2020-09-22 | 2023-05-23 | 嘉兴市农业科学研究院 | Stropharia rugoso-annulata liquid strain incubator and culturing method |
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