CN112076221B

CN112076221B - Desensitizing paste and preparation method and application thereof

Info

Publication number: CN112076221B
Application number: CN202011014331.2A
Authority: CN
Inventors: 王崇福; 王葵
Original assignee: Sichuan Tianfu Meticulous Chemical Co ltd
Current assignee: Sichuan Tianfu Meticulous Chemical Co ltd
Priority date: 2020-09-24
Filing date: 2020-09-24
Publication date: 2022-02-22
Anticipated expiration: 2040-09-24
Also published as: CN112076221A

Abstract

The invention discloses desensitization paste, which comprises 0.3-0.5 part of Chinese gall extract, 0.05-0.3 part of chlorhexidine acetate, 5-10 parts of honeycomb extract, 50-60 parts of calcium powder, 5-15 parts of strontium chloride, 5-15 parts of potassium nitrate, 10-15 parts of sodium fluoride and 30-56 parts of pharmaceutically acceptable auxiliary materials. The desensitizing paste provided by the invention is added with the active extract of Chinese gall, has the functions of astringing and precipitating protein, can block and close dentinal tubules, and can be used for fundamentally desensitizing. In addition, the combined application of the active ingredients of the Chinese gall, the chlorhexidine acetate and the honeycomb extract has obvious synergistic effect, can greatly improve the tooth desensitization and sterilization effects of the product, particularly has obvious inhibition effect on streptococcus mutans forming dental caries, and has the effect of preventing and treating various oral diseases.

Description

Desensitizing paste and preparation method and application thereof

Technical Field

The invention belongs to the technical field of oral medical care, and particularly relates to desensitization paste for preventing and/or treating oral diseases and a preparation method thereof.

Background

With the remarkable improvement of the living standard of the substances of people and the continuous improvement of the consciousness of oral health care, the prevention of dental diseases, the treatment of dental diseases and the health care of teeth become more and more concerned problems. The oral cavity of a human body is a relatively complex internal environment and contains a large amount of harmful bacteria, and if the oral cavity is not nursed properly, a plurality of problems such as dentin hypersensitivity, dental ulcer, gingival bleeding, gingivitis and the like are easy to occur, so that the food intake and the daily life of a patient are influenced.

Dentine hypersensitivity is a subjective symptom caused by external stimuli of exposed dentine, and is represented by tooth soreness and pain caused by external stimuli such as temperature (cold, heat), chemical (sour, sweet), mechanical (rubbing or hard biting) and the like. In oral diseases, dentin hypersensitivity belongs to a common disease, 8-30% of people statistically suffer from dentin hypersensitivity, and particularly middle-aged people of about 40 years old suffer from high-grade dental hypersensitivity. The principle of dentinal hypersensitivity is thought to be that there is dentinal fluid in the dentinal tubules, and when there is external stimulus, the flow rate of dentinal fluid changes, causing swelling and compression of the dentinal cells and their projections, and mechanically stirring the nerve endings of the dental pulp to cause pain.

Based on the theoretical basis, laser desensitization treatment or treatment by using medicaments such as fluoride, strontium chloride, silver ammonium fluoride, silver iodide and the like is usually adopted clinically, but the medicaments have the advantages and the disadvantages, the treatment effect is not ideal, the treatment effective rate is low, the effective maintaining time is short, the recurrence rate is high, and the methods cannot fundamentally treat the allergic symptoms of the dentin of a patient. Therefore, there is a need for a desensitizing paste which has a long-lasting effect and is not likely to recur after treatment, and which can fundamentally prevent and treat dentinal hypersensitivity of patients.

Secondly, in the clinical practice of the stomatology, caries is also a widespread chronic disease, the most common disease that endangers human oral health. Caries is most different from other diseases in that it is cumulative, and after it has progressed to some extent, there is little to no chance of restoration other than artificial restoration, which would ultimately lead to pain and tooth loss if left untreated. It is now internationally accepted that caries is a multifactorial disease. A large number of studies have demonstrated that caries can be prevented and a significant therapeutic effect has been achieved. The principle of preventing caries is to reduce the pathogenic factors of caries, enhance the anti-caries ability, control bacterial plaque and pay attention to the diet hygiene. However, because of the complex pathogenic factors of caries, comprehensive caries prevention measures must be taken to prevent or reduce the occurrence of caries. Clinical practice proves that the desensitizing paste can effectively prevent and treat the caries.

Dental plaque is an ecological environment with bacteria as the main component that adheres firmly to the tooth surface. The ecology of dental plaque is extremely complex, and bacteria are constantly growing and developing, wherein complex metabolism of substances is carried out, and the co-habitation, competition and antagonism of bacteria play an important role in the composition and properties of plaque, and the like. Dental plaque is the cause of both caries and periodontal disease, but the nature of the plaque responsible for caries and periodontal disease is different, as are the tissues and environment in which they act. The main cariogenic components are streptococcus mutans and lactobacillus which can produce and resist acid, and the like, so that tooth tissues are demineralized, and organic substances are decomposed. Periodontal disease is caused mainly by anaerobic bacteria that produce metabolites such as toxins and enzymes, which damage the periodontal tissues. The desensitizing paste can effectively inhibit various bacteria in the oral cavity and clean the oral cavity, thereby reducing the harm of pathogenic bacteria to the oral health.

In conclusion, the high-quality desensitization paste product not only can effectively cure the dentin hypersensitivity, but also can play a significant role in preventing and treating dental caries and dental plaque.

Disclosure of Invention

The desensitizing paste is added with Chinese gall active extract, and the Chinese gall contains tannic acid, has the functions of astringency and protein precipitation, can block and close dentinal tubules, forms a layer of protective film to protect oral ulcer surfaces and oral mucosa, and can be used for fundamentally desensitizing. In addition, chlorhexidine acetate is added in the formula of the desensitizing paste, so that the stability of the desensitizing paste can be further enhanced, the desensitizing effect is more durable, and the symptoms are not easy to relapse after the desensitizing paste is used. In addition, the desensitization paste provided by the invention is added with a certain proportion of honeycomb extract, and the honeycomb extract has the effects of dispelling wind, easing pain, counteracting toxic substances, resolving masses, killing parasites, relieving itching and resisting allergy. The inventor of the invention unexpectedly discovers that the combined application of the Chinese gall active ingredient, the chlorhexidine acetate and the honeycomb extract has an obvious synergistic effect, can greatly improve the bacteriostatic and bactericidal effects of the product, particularly has an obvious inhibiting effect on streptococcus mutans forming dental caries, and has obvious advantages of preventing and treating dental caries and removing dental plaque. And the combined application of the active ingredients of the Chinese gall, the chlorhexidine acetate and the honeycomb extract can assist strontium chloride, sodium fluoride and other ingredients to play a better role in desensitizing teeth.

In a first aspect, the invention provides desensitization paste, which comprises 0.3-0.5 part of Chinese gall extract, 0.05-0.3 part of chlorhexidine acetate and 5-10 parts of honeycomb extract.

Preferably, the desensitization paste further comprises 50-60 parts of calcium powder, 5-15 parts of strontium chloride, 5-15 parts of potassium nitrate, 10-15 parts of sodium fluoride and 30-56 parts of pharmaceutically acceptable auxiliary materials.

In the invention, the pharmaceutically acceptable auxiliary materials comprise one or a combination of more than two of thickening agent, humectant, foaming agent and flavoring agent.

The thickening agent is one or a composition of more than two of guar gum, pectin, sodium alginate, cyclodextrin, sodium carboxymethyl starch, carboxymethyl cellulose and Arabic gum.

The humectant is one or a composition of more than two of glycerin, sorbitol and propylene glycol.

The foaming agent is one or a composition of two of sodium dodecyl sulfate and sodium cocoyl glycinate.

The flavoring agent is one or more of oleum Terebinthinae, oleum Caryophylli and oleum Menthae Dementholatum.

In a preferred embodiment of the invention, the desensitizing paste comprises the following components in parts by mass: 50-60 parts of calcium powder, 5-15 parts of strontium chloride, 5-15 parts of potassium nitrate, 10-15 parts of sodium fluoride, 0.3-0.5 part of nutgall extract, 0.05-0.3 part of chlorhexidine acetate, 5-10 parts of honeycomb extract, 0.8-1.5 parts of carboxymethyl cellulose, 0.5-1 part of Arabic gum, 20-30 parts of glycerol, 8-15 parts of sorbitol, 2-6.5 parts of sodium dodecyl sulfate and 0.8-1.5 parts of turpentine.

Preferably, the desensitizing paste comprises the following components in parts by mass: 50 parts of calcium powder, 10 parts of strontium chloride, 10 parts of potassium nitrate, 10 parts of sodium fluoride, 0.5 part of nutgall extract, 0.1 part of chlorhexidine acetate, 5 parts of honeycomb extract, 1 part of carboxymethyl cellulose, 1 part of Arabic gum, 20 parts of glycerol, 10 parts of sorbitol, 3 parts of sodium dodecyl sulfate and 1 part of turpentine.

The gallnut extract of the present invention may be purchased commercially or prepared by itself. In a preferred embodiment of the invention, the gallnut extract is prepared by the following method: pulverizing Galla chinensis, extracting with 3-4 times of deionized water at 60-70 deg.C in water bath for 10-12 hr, shaking, filtering, repeating the previous extraction of the residue, mixing filtrates, decolorizing with activated carbon, filtering to obtain Galla chinensis extractive solution, and concentrating into paste.

The honeycomb extract liquid is obtained by extracting honeycombs by using 75-95% ethanol solution as an extracting agent.

In a second aspect, the present invention provides a method of preparing a desensitizing paste, the method comprising the steps of:

(1) mixing calcium powder, strontium chloride, potassium nitrate, sodium fluoride, Galla chinensis extract, chlorhexidine acetate, nidus Vespae extractive solution, carboxymethyl cellulose, and sodium dodecyl sulfate at a certain proportion, and dispersing into glycerol;

(2) adding acacia, sorbitol and turpentine into the dispersion system, and mixing to obtain desensitizing paste.

Preferably, the desensitizing paste is placed in a hose for use.

In a third aspect, the present invention provides the use of a desensitizing paste in the prevention and/or treatment of oral diseases including, but not limited to, dentinal hypersensitivity, caries, plaque, gum bleeding, canker sores, gingivitis.

Preferably, the oral diseases are dentin hypersensitiveness, caries and dental plaque.

The technical advantages of the invention are as follows: (1) the desensitizing paste is added with Chinese gall active extract, wherein the Chinese gall contains tannic acid, has the functions of astringency and protein precipitation, can block and close dentinal tubules, and can be used for fundamentally desensitizing; (2) the paste containing the active ingredient of the gallnut, which is prepared by the invention, forms a layer of protective film after being applied to protect the oral ulcer surface and the oral mucosa, thereby effectively preventing the oral problems; (3) the chlorhexidine acetate is added in the formula, so that the stability of desensitization paste can be further enhanced, the desensitization effect is more durable, and the symptoms are not easy to relapse after the desensitization paste is used; (4) the nidus Vespae extract has effects of dispelling pathogenic wind, relieving pain, removing toxic substance, resolving hard mass, killing parasite, relieving itching and resisting allergy, and can cooperate with Galla chinensis traditional Chinese medicine active ingredient and chlorhexidine acetate to exert better tooth desensitization and antibacterial effects; (5) the combined application of the active ingredients of the Chinese gall, the chlorhexidine acetate and the honeycomb extract has obvious synergistic effect, can greatly improve the bacteriostatic and bactericidal effects of the product, particularly has obvious inhibiting effect on streptococcus mutans forming dental caries, and has obvious advantages of preventing and treating dental caries and removing dental plaque.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

EXAMPLE 1 preparation of desensitizing paste

S1: uniformly mixing 50 parts of calcium powder, 5 parts of strontium chloride, 5 parts of potassium nitrate, 10 parts of sodium fluoride, 0.3 part of nutgall extract, 0.05 part of chlorhexidine acetate, 5 parts of honeycomb extract, 0.8 part of carboxymethyl cellulose and 2 parts of sodium dodecyl sulfate, and dispersing into 20 parts of glycerol;

s2: adding 0.5 part of Arabic gum, 8 parts of sorbitol and 0.8 part of turpentine into the dispersion system, and uniformly mixing to obtain the desensitizing paste.

EXAMPLE 2 preparation of desensitizing paste

S1: uniformly mixing 60 parts of calcium powder, 15 parts of strontium chloride, 15 parts of potassium nitrate, 15 parts of sodium fluoride, 0.5 part of nutgall extract, 0.3 part of chlorhexidine acetate, 10 parts of honeycomb extract, 1.5 parts of carboxymethyl cellulose and 6.5 parts of sodium dodecyl sulfate, and dispersing into 30 parts of glycerol;

s2: adding 1 part of Arabic gum, 15 parts of sorbitol and 1.5 parts of turpentine into the dispersion system, and uniformly mixing to obtain the desensitizing paste.

EXAMPLE 3 preparation of desensitizing paste

S1: uniformly mixing 50 parts of calcium powder, 10 parts of strontium chloride, 10 parts of potassium nitrate, 10 parts of sodium fluoride, 0.5 part of nutgall extract, 0.1 part of chlorhexidine acetate, 5 parts of honeycomb extract, 1 part of carboxymethyl cellulose and 3 parts of sodium dodecyl sulfate, and dispersing into 20 parts of glycerol;

s2: adding 1 part of Arabic gum, 10 parts of sorbitol and 1 part of turpentine into the dispersion system, and uniformly mixing to obtain the desensitizing paste.

Comparative example 1 preparation of desensitizing paste

The preparation method and the preparation raw materials are the same as example 3, and only the raw materials do not contain the nutgall extract.

Comparative example 2 preparation of desensitizing paste

The preparation method and the preparation raw materials are the same as example 3, except that the raw materials do not contain chlorhexidine acetate.

Comparative example 3 preparation of desensitizing paste

The preparation method and the preparation raw materials are the same as example 3, except that the raw materials do not contain the honeycomb extract.

Firstly, the physical and chemical properties of the desensitization paste prepared by the invention are detected

The desensitizing pastes prepared in the embodiments 1 to 3 of the invention are subjected to physical and chemical property detection, which is performed by the detection center of medical instruments in Sichuan province, and the detection reports are shown in the following table:

TABLE 1 desensitization paste test report

(II) cell toxicity test of desensitizing paste prepared by the invention

The desensitizing pastes prepared in examples 1-3 of the present invention were subjected to cytotoxicity tests, which were conducted by the health monitoring and inspection center of Sichuan province.

Test materials

Will give an instant disclosureWeighing a sample according to the proportion of 0.2g/ml in the desensitization paste prepared by Ming, putting the sample into a sterile container, adding sterilized normal saline, sealing, and continuously leaching for 24 hours at the temperature of 37 +/-2 ℃ to obtain a sample leaching solution. When testing, 100mm²The sterile filter paper sheet absorbs 0.1ml of sample leaching liquor as a test sample, a negative sample adopts sterile physiological saline, and a positive sample is diluted phenol.

Test method

Taking mouse L-929 fibroblast strain, culturing and passing for 48 hours, and preparing into 3X 10 after the action of digestive juice⁵Cell suspension at 90 mm/ml²Adding 10ml cell suspension into the plate, and adding 5% CO at 37 deg.C₂Cultured in an incubator for 24 hours to form a well-growing but less dense reticular monolayer of cells. Eagle agar medium (10ml medium/0.1 ml neutral red) mixed with neutral red staining solution was added to the dish, and the plate was covered with a monolayer of cells, and the prepared test sample and negative and positive samples were placed on the agar surface, and cultured for 24 hours to observe the results.

Test results

The desensitization pastes prepared in examples 1-3 of the present invention were used as the culture dishes for the test samples without cell lysis, and the negative control without cell lysis and the positive control with cell lysis of 60%. The test results prove that the desensitization paste prepared by the invention has no cytotoxicity.

Effect example 1 therapeutic Effect of desensitizing paste on dentinal hypersensitivity

Purpose of the experiment

The treatment effect of the desensitization paste prepared by the invention on the dentin hypersensitive patients is verified.

Test method

During the test period, other toothpaste, gargle and desensitizer, antibiotics, immune preparation and the like are not used, and the treatment affecting the tooth sensitivity such as cleaning, scraping, filling of living marrow and teeth and the like is not carried out. 140 dentin hypersensitivity patients were randomly divided into 7 groups of 20 persons, and the degree of tooth hypersensitivity was examined by blowing cold air vertically for 1s at a distance of 5mm from the buccal surface of sensitive teeth of the subjects. 3g of desensitizing paste prepared in examples 1 to 3 and comparative examples 1 to 3 were applied to sensitive tooth surfaces of subjects, respectively, and after holding for 3min, the mouth was rinsed, the desensitizing effect was immediately checked with a probe, and the sensitivity was recorded. The control group was given 3g of a commercially available olmin tooth-cleaning desensitizing agent and sensitivity was recorded using the same method. After the test, each group of subjects was given a corresponding desensitization paste box, and the subjects were instructed to use once in the morning and at night, and the treatment was repeated before 1 week, and then the desensitization effect was checked again with a probe, and the sensitivity was recorded.

Examination standard for degree of tooth sensitivity

Mild: slight sensitivity or acid feeling to cold stimulation and probes in allergic regions;

medium: the cold stimulation is painful, the probe for the allergic area is painful, and the probe can endure the probe inspection.

And (3) severe degree: the mouth cannot be rinsed with cold and hot water, the mouth is extremely sensitive to sour and sweet foods, and the probe allergic area is severe in ache and cannot tolerate.

Criteria for efficacy assessment

The effect is shown: after the patients mainly take the medicine, the cold, hot, sour and sweet stimulation is carried out to eliminate the ache symptom, and the symptom completely or basically disappears when the original allergic area is checked by a probe;

the method has the following advantages: the cold, hot, sour and sweet stimulation and ache symptoms of the patient after the administration are relieved compared with those before the administration, and the symptoms are relieved or improved when the original allergic area is inspected by a probe;

and (4) invalidation: the symptoms are not improved after the medicine is taken.

The method for calculating the treatment significant efficiency and the effective rate comprises the following steps:

the significant efficiency (%) — significant number/total number of treatments × 100%;

effective rate (%) - (number of significant effect + number of effective effect)/total number of treatments × 100%.

Test results

TABLE 1 results of clinical trials of desensitizing pastes for treatment of dentinal hypersensitivity

As can be seen from the test results, the immediate treatment effect of the desensitizing paste prepared in examples 1-3 is more remarkable for patients with dentin hypersensitivity than for comparative examples 1-3, especially for the desensitizing paste prepared in example 3, and the immediate effective rate can reach 100%. In comparison, the desensitizing pastes prepared in comparative examples 1-3 had an immediate effectiveness of 65-70% including the commercially available Orpimento tooth cleaning desensitizer. After 1 week of treatment, the effective rate of the desensitization paste prepared by the invention for tooth desensitization reaches 100 percent, which shows that the desensitization paste provided by the invention has obvious curative effect on dentin hypersensitivity. Comparative examples 1 to 3 were prepared by removing the Galla chinensis extract, chlorhexidine acetate and the nidus Vespae extract, respectively, on the basis of example 3, and as a result, the therapeutic effects were not as good as those of example 3, which shows that the desensitizing paste provided by the present invention can achieve the best desensitizing effect only by using the Galla chinensis extract, chlorhexidine acetate and the nidus Vespae extract in combination. No side effects associated with the tested products were found in the experiments.

Effect example 2 persistent therapeutic effects of desensitizing paste on dentinal hypersensitivity

Purpose of the experiment

The durability of the treatment effect of the desensitization paste prepared by the invention on the dentin hypersensitive patients is verified.

Test method

Effect example 1 a trial was continued with 140 dentinal hypersensitivity patients, and the subjects in each group continued to use the corresponding desensitizing paste for 2 weeks, once each in the morning and evening, and 3 weeks total before and after treatment for a double visit, and the desensitizing effect was checked again with the probe and the sensitivity was recorded. After the end of the follow-up visit, the patients were instructed to stop using the desensitizing paste, and after 10 days, the follow-up visit was carried out again, and the probability of recurrence of symptoms in each group was recorded.

Test results

TABLE 2 results of clinical trials of desensitizing pastes for treating dentinal hypersensitivity

The test results show that the effective rate of tooth desensitization of the patients with dentin hypersensitivity reaches 100 percent after the desensitization paste 3 prepared in the examples 1-3 is applied, the effect-showing rate results are very considerable, and the recurrence probability of symptoms is 0 after the administration is stopped for 10 days, which shows that the desensitization paste provided by the invention has lasting and effective treatment effect on dentin hypersensitivity. Compared with the commercially available olmesan hydrochloride group, the effective rate is not changed after 3 weeks of treatment, but the effective rate is reduced from 60% to 55%, which is probably because the medicine has tolerance to the affected part and has no effect after being continuously used, the symptom recurrence probability reaches 45% after the use of the olmesan hydrochloride is stopped, and the curative effect is not lasting. In addition, the inventor unexpectedly discovers that only the desensitization paste contains the active extract of the Chinese gall, the honeycomb extract and the chlorhexidine acetate, so that the prepared desensitization paste has more durable curative effect on dentin hypersensitive patients and has lower probability of symptom relapse after stopping taking the desensitization paste for 3 weeks. The inventor thinks that the Chinese gall extract contains tannic acid, has stronger convergence effect, can block and close dentinal tubules and fundamentally relieves the allergic symptoms; the nidus Vespae extract contains multiple microelements, and propolis in the extract has effect of enhancing immunity; in addition, chlorhexidine acetate has a strong sterilization and disinfection effect, helps other effective components to play a role, and slows down the recurrence of symptoms. Small molecule effective substances such as strontium chloride, sodium fluoride and the like in the desensitization paste are stably dispersed in viscous paste formed by gallnut traditional Chinese medicine active extract, honeycomb extract and thickening agent, and a layer of protective film can be formed on the surface of teeth in the application process, so that the desensitization paste has a longer curative effect of relieving dentin hypersensitivity.

Effect example 3 preventive or therapeutic Effect of desensitizing paste on caries

Purpose of the experiment

Streptococcus mutans is a common dental caries pathogenic bacterium, and the test indirectly shows the effect of the desensitizing paste on preventing and treating dental caries by verifying the inhibiting effect of the desensitizing paste on the Streptococcus mutans.

Test method

(1) Subculturing streptococcus mutans to form streptococcus mutans flora, and diluting streptococcus mutans bacterial liquid in logarithmic phase to 1.0 × 10⁶CFU/mL, 0.1mL of bacterial liquid is dripped on the sterilized 24-well plate, and the 24-well plate is put into an anaerobic incubator to be cultured for 24 hours at 37 ℃ without the periodAdding fresh culture solution, and recording the quantity of streptococcus mutans at the moment;

(2) 0.1g of the desensitizing paste prepared in examples 1 to 3 and comparative examples 1 to 3 of the present application and a commercially available olsensitive tooth-cleaning desensitizing agent were diluted 10-fold with distilled water, and each was dropped into a 24-well plate which had been cultured, to perform a bacteriostatic test, and the number of Streptococcus mutans was recorded after culturing for 24 hours under the same conditions.

Test results

The percent reduction in the number of Streptococcus mutans bacteria before and after desensitization paste incubation is shown in the following table:

TABLE 3 inhibitory Effect of desensitizing pastes on Streptococcus mutans

Test group	Percentage of bacterial inhibition
		Example 1	34.8％
Example 2	38.0％
		Example 3	47.3％
Comparative example 1	20.4％
		Comparative example 2	12.5％
Comparative example 3	26.1％
		'Amin Qing' group	21.7％

As can be seen from the data in the table, the desensitization pastes prepared in the examples 1 to 3 of the invention have relatively good inhibition effect on streptococcus mutans, and the optimal inhibition rate can reach 47.3%. Compared with the example 3, when the gallnut traditional Chinese medicine active ingredient, the chlorhexidine acetate or the honeycomb extract liquid are lacked in the formula, the inhibition effect of the desensitizing paste on the streptococcus mutans is reduced, which shows that the inhibition effect of the gallnut traditional Chinese medicine active ingredient, the chlorhexidine acetate and the honeycomb extract liquid on the streptococcus mutans is the best when the active ingredient, the chlorhexidine acetate and the honeycomb extract liquid are used together. In addition, the desensitization paste prepared by the invention has better bacteriostatic effect on streptococcus mutans than the current marketed oximin tooth-cleaning desensitizer, which shows that the desensitization paste prepared by the invention has better prevention and treatment effect on dental caries.

Effect example 4 Desensitizing paste removal effectiveness on dental plaque

Purpose of the experiment

The desensitizing paste prepared according to the present invention was validated for plaque removal efficacy.

Test method

(1) 105 subjects were randomized into 7 groups of 15 subjects who brushed 1 time each day, morning and evening, 2g of desensitizing paste per day for 3 months using each of the examples 1-3, comparative examples 1-3, and commercially available olmin tooth-cleaning desensitizing agents, respectively, using horizontal tremor method; the subjects were observed for a change in plaque index (PLI) before and after use of the test desensitizing paste.

(2) The plaque index reduction rate was calculated as (average plaque index before use-average plaque index after 3 months of use)/average plaque index before use x 100%.

Test results

TABLE 4 Desensitizing paste's plaque removal Effect

The core of removing dental plaque is to remove extracellular polymer secreted by microorganisms, the extracellular polymer mainly comprises protein, polysaccharide, nucleic acid, uronic acid, lipid, humic acid, amino acid and the like, and the essence of removing dental plaque is to remove essential substances for growth, inhibit adhesion of microorganisms and promote detachment. As can be seen from the above table, the desensitizing paste prepared by the invention has a mean plaque reduction rate of 65-82%, while the commercial Orpimox tooth-cleaning desensitizer has a plaque removal rate of only 44%. Compared with example 3, when the desensitizing paste formulation does not contain Chinese gall traditional Chinese medicine active ingredients, chlorhexidine acetate and honeycomb extract, the average dental plaque reduction rate is reduced from 81.5 percent to 53.8 percent, 42.3 percent and 53.8 percent respectively. The active ingredients of the Chinese gall, the chlorhexidine acetate and the honeycomb extract are indispensable effective ingredients for exerting the effect of removing dental plaque, and the best effect of removing dental plaque can be achieved only by combining the three ingredients, but none is indispensable.

Effect example 5 desensitization paste stability test

Purpose of the experiment

The storage stability of the desensitization paste prepared by the invention is verified.

Test method

The desensitizing pastes prepared in examples 1 to 3 of the present invention and comparative examples 1 to 3 and a commercially available oximin tooth-cleaning desensitizer were examined for their properties of products, their mildewing and their hardening after 6 months of storage at room temperature and 6 months of storage in an oven at 42 ℃.

Test results

TABLE 5 desensitization paste stability test results

As can be seen from the test results in the table above, the desensitizing pastes prepared in examples 1-3 of the present invention do not have the phenomena of delamination and precipitation, mildew and hardening after being stored for 6 months at normal temperature or at high temperature of 42 ℃, which indicates that the product stability is good. When no gallnut extract is added into the product, the product can be hardened after being stored for 6 months at the high temperature of 42 ℃, and when chlorhexidine acetate is not added into the product, slight peculiar smell can be generated after being stored for 6 months at the high temperature of 42 ℃, which indicates that the product has a mildew tendency. The o-Min-Qing desensitizer sold in the market can be stable within 6 months of storage life at normal temperature, but precipitates, mildews and hardens at the high temperature of 42 ℃. The inventors of the present invention have analyzed the above results and have considered that the honeycomb extract contains components such as beeswax, propolis, honeycomb oil and the like, and particularly the content of propolis is relatively high. Propolis not only has the effects of relieving pain, resisting bacteria, diminishing inflammation and resisting virus, but also is a resin-like substance and can increase the stability of small-molecular effective components such as strontium chloride, sodium fluoride and the like in desensitizing paste. The chlorhexidine acetate has antibacterial and disinfectant effects, and can slow down the mildew process of desensitizing paste during long-term storage. Hardening of the paste during storage of the desensitizing paste is a common problem because the desensitizing paste contains certain amounts of strontium chloride, potassium nitrate, sodium fluoride, etc. and even the addition of humectant glycerin does not ensure long-term stability of the paste. In the stability experiment, the inventor unexpectedly finds that the Chinese gall extract can prevent the desensitization paste from hardening during long-term storage, and the analysis reason is probably that the Chinese gall extract is a pasty substance and can wrap the effective components in the desensitization paste or enhance the effect of a humectant.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A desensitizing paste comprises the following components in parts by mass: 50-60 parts of calcium powder, 5-15 parts of strontium chloride, 5-15 parts of potassium nitrate, 10-15 parts of sodium fluoride, 0.3-0.5 part of nutgall extract, 0.05-0.3 part of chlorhexidine acetate, 5-10 parts of honeycomb extract, 0.8-1.5 parts of carboxymethyl cellulose, 0.5-1 part of Arabic gum, 20-30 parts of glycerol, 8-15 parts of sorbitol, 2-6.5 parts of sodium dodecyl sulfate and 0.8-1.5 parts of turpentine;

the preparation method of the desensitizing paste comprises the following steps:

2. The desensitization paste of claim 1, wherein said desensitization paste is placed in a hose for use.

3. Use of a desensitizing paste according to any of claims 1-2 in the manufacture of a medicament for the prevention and/or treatment of oral diseases such as dentinal hypersensitivity, caries, dental plaque.