CN112042475A - Formula, production process and application of mycorrhizal bacterial manure matrix of rivet mushroom - Google Patents
Formula, production process and application of mycorrhizal bacterial manure matrix of rivet mushroom Download PDFInfo
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- CN112042475A CN112042475A CN202010833948.0A CN202010833948A CN112042475A CN 112042475 A CN112042475 A CN 112042475A CN 202010833948 A CN202010833948 A CN 202010833948A CN 112042475 A CN112042475 A CN 112042475A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- Chemical & Material Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a formula, a production process and application of a matrix of a mycorrhizal bacterial fertilizer of a rivet mushroom, which comprises 500 g-800 g of the mycorrhizal bacterial strain of the rivet mushroom, 20 g of the cottonseed hull, 150 g of the compound fertilizer, 5 g of the mycorrhizal fungi and 100 g of talcum powder, wherein the mycorrhizal bacterial fertilizer of the rivet mushroom is mixed with pine needle soil and a seedling substrate, water is added for stirring to obtain the matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom, and through various enzymes secreted by mycorrhizal fungi in a metabolic process, minerals or inorganic matters in the soil can be synthesized into organic matters helpful for tree growth, growth hormones and regulators for promoting plant growth can be secreted, the growth and development of the plant can be promoted, the stress resistance of the plant can be enhanced, and the survival rate can be improved.
Description
Technical Field
The invention belongs to the technical field of agricultural planting, and particularly relates to a formula, a production process and application of a mycorrhizal bacterial fertilizer matrix for rivet mushrooms.
Background
The Chinese pine is a main afforestation tree species in our province, but due to the influence of drought factors (the rainfall in our province is 400-650 mm, but the distribution is uneven), the survival rate of Chinese pine seedlings in the afforestation of the Chinese pine in the barren mountains is low, the afforestation and greening results in our province are seriously influenced, and particularly in recent years, the requirements of the construction of ecological provinces are more urgent to the greening requirements of our province. However, the existing Chinese pine nursery stocks lack corresponding bacterial manure substrates for growth.
Disclosure of Invention
1. Technical problem to be solved by the invention
The invention aims to solve the problem that the existing Chinese pine nursery stock is lack of a corresponding bacterial manure matrix for growth.
2. Technical scheme
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention relates to a formula of a mycorrhizal bacterial fertilizer matrix for rivet mushroom, which is characterized in that; comprises 500-800 g of the rivet mushroom strain, 20 jin of the cotton seed hull, 150 g of the compound fertilizer, 5 g of the mycorrhizal and 100 g of the talcum powder.
A production process of a mycorrhizal bacterial fertilizer matrix of a rivet mushroom comprises the following steps:
s100, separating mycorrhizal fungi of the rivet mushroom fungi;
s200, culturing the separated mycorrhiza strains;
s300, propagating the cultured mycorrhizal strains;
s400, preparing a substrate;
s500, preparing a matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom.
Preferably, the step S200 of culturing the separated mycorrhizal fungi specifically includes inoculating the separated mycorrhizal fungi to a slant culture medium, grouping the slant culture medium into a group according to 5 test tubes, wrapping the ends of the test tubes with sterile kraft paper, tightening with rubber bands, making identification marks on the kraft paper, and then placing all the test tubes into a constant temperature and humidity incubator with a temperature and humidity of 25 ℃ and a humidity of 40% to culture the fungi.
Preferably, in step S300, the cultured mycorrhiza species is inoculated again and cultured in a constant temperature and humidity chamber, the temperature is adjusted to 25 ℃, and the humidity is adjusted to 60%.
Preferably, in the step S300, the method further includes a second-stage strain propagation, wherein the cultured mycorrhizal strain is inoculated into 10 second-stage culture bottles according to each test tube, the volume of each second-stage culture bottle is 0.5 liter, the inoculation amount is about 1 to 3 grams, the inoculation position is located at the center of each second-stage culture bottle, and when hyphae in the second-stage culture bottle overgrow, the hyphae are reserved for production and inoculation of the mycorrhizal fungi agent.
Preferably, the substrate in the step 400 is prepared by crushing the main raw material of the substrate according to the formula of the substrate, fermenting for 15-20 hours, uniformly spraying water by using a sprayer, uniformly stirring for later use, controlling the humidity of the substrate to be 50-60%, and filling the stirred substrate into a special material bag for sealing.
Preferably, the preparation of the mycorrhizal bacterial fertilizer matrix of the pleurotus ferulae in the step 500 is to inoculate hyphae in the secondary culture bottle of the step 300 to the matrix in a special material bag after the hyphae are overgrown, so that the mycorrhizal bacterial fertilizer matrix of the pleurotus ferulae is obtained.
Preferably, the pH of the matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom is controlled to be 5-6.
An application of a mycorrhizal bacterial fertilizer matrix of a rivet mushroom in promoting the growth direction of a Chinese pine seedling.
3. Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
the invention relates to a formula, a production process and application of a matrix of a mycorrhizal bacterial fertilizer of a rivet mushroom, which comprises 500-800 g of rivet mushroom strains, 20 jin of cotton seed shells, 150 g of compound fertilizer, 5 g of mycorrhizal and 100 g of talcum powder. The mycorrhizal bacterial manure of the pleurotus ferulae, the pine needle soil and the seedling raising substrate are mixed, water is added and stirred to obtain the mycorrhizal bacterial manure substrate of the pleurotus ferulae, various enzymes secreted by mycorrhizal fungi in the metabolic process can synthesize inorganic matters in ores or soil into organic matters helpful for tree growth, and can secrete growth hormone and regulator for promoting plant growth, promote plant growth and development, enhance the stress resistance of plants and improve the survival rate. .
Drawings
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
In order to facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which may be embodied in many different forms and are not limited to the embodiments described herein, but rather are provided for the purpose of providing a more thorough disclosure of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; as used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
Referring to the attached drawing 1, the formula, the production process and the application of the mycorrhizal bacterial fertilizer matrix for the pleurotus ostreatus comprise pleurotus ostreatus strains, cottonseed hulls, compound fertilizer, moroxydine and talcum powder, wherein the content of each component is 500-800 g of the pleurotus ostreatus strains, 20 jin of the cottonseed hulls, 150 g of the compound fertilizer, 5 g of the moroxydine and 100 g of the talcum powder.
A production process of a mycorrhizal bacterial fertilizer matrix of a rivet mushroom comprises the following steps:
s100, separating mycorrhizal fungi of the rivet mushroom fungi;
s200, culturing the separated mycorrhiza strains;
s300, propagating the cultured mycorrhizal strains;
s400, preparing a substrate;
s500, preparing a matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom.
The step S200 of culturing the separated mycorrhizal strain specifically comprises the steps of inoculating the separated mycorrhizal strain to a slant culture medium, enabling the slant culture medium to form a group according to 5 test tubes, wrapping the ends of the test tube with sterile kraft paper, binding with rubber bands, making identification marks on the kraft paper, and then putting all the test tubes into a constant-temperature constant-humidity incubator with the temperature of 25 ℃ and the humidity of 40% for strain culture.
In step S300, the cultured mycorrhizal strain is inoculated again and cultured in a constant temperature and humidity box, the temperature is adjusted to 25 ℃, and the humidity is adjusted to 60%.
And step S300, carrying out secondary strain propagation expansion, inoculating 10 secondary culture bottles with the cultured mycorrhizal strains according to each test tube, wherein the volume of each secondary culture bottle is 0.5 liter, the inoculation amount is about 1-3 g, the inoculation part is positioned at the center of each bottle, and when hyphae in the secondary culture bottles grow to be full, the hyphae are reserved for production and inoculation of the mycorrhizal fungi agent.
In the step 400, the substrate is specifically prepared by crushing the main raw materials of the substrate according to the formula of the substrate, fermenting for 15-20 hours, uniformly spraying water by using a spraying machine, uniformly stirring for later use, controlling the humidity of the substrate to be 50-60%, and filling the stirred substrate into a special material bag for sealing.
The step 500 of preparing the mycorrhizal bacterial fertilizer matrix of the pleurotus ostreatus is to inoculate the hypha in the secondary culture bottle of the step 300 to the matrix in the special material bag after the hypha grows full, so that the mycorrhizal bacterial fertilizer matrix of the pleurotus ostreatus is obtained.
Preferably, the pH of the matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom is controlled to be 5-6.
An application of a mycorrhizal bacterial fertilizer matrix of a rivet mushroom in promoting the growth direction of a Chinese pine seedling.
Through tests, after the rivet mushroom fungi is inoculated, various indexes of the Chinese pine are influenced, such as: the average seedling height of the inoculated pine seedlings is increased by 128 percent compared with that of the control seedlings; the average ground diameter is 126 percent of that of the control seedlings; biomass was 121% of control seedlings, etc. After the pinus tabulaeformis is inoculated with the rivet mushroom, the chlorophyll a is higher than the contrast, the chlorophyll b content is higher than the contrast, and the total chlorophyll content is also higher than the contrast. The rivet mushroom can improve the photosynthetic rate of the Chinese pine, and the relative photosynthetic rate is increased by 18% compared with a control. Experimental results show that the total nitrogen and total phosphorus contents of stems, leaves and roots of the seedlings of the Chinese pine forming the ectotrophic mycorrhiza are obviously higher than those of the seedlings of the Chinese pine without the ectotrophic mycorrhiza, and particularly the total phosphorus contents of the stems and leaves are most obvious. Comparing the total nitrogen and phosphorus contents of the whole Chinese pine seedling, the formation of the ectomycorrhiza can be seen to have a stronger promoting effect on phosphorus absorption than nitrogen absorption, such as: the total nitrogen content of the root system of the Chinese pine seedling is 1.6 times of that of the contrast, and the total phosphorus content is 2.1 times; the total nitrogen content of the stem leaves of the Chinese pine seedlings is 4.5 times of that of the control, and the total phosphorus content reaches 6.7 times. Rivet mushroom fungus absorption K+Is very powerful. Wherein the Km value is 15.31 mu mol/L, Imax35.07 mu mol/g.DW.h, Cmm1.29 mu mol/L; small Km value, for K+Has a strong affinity of (C)mmThe value was also very low, indicating that the Pholiota nameko fungus was able to survive at KTen pieces of clothAbsorption of K in very low concentration solutions+。
Drought-resisting texture research test of hypsizygus marmoreus fungus on pinus tabulaeformis seedlings
The Chinese pine is a main afforestation tree species in our province, but due to the influence of drought factors (the rainfall in our province is 400-650 mm, but the distribution is uneven), the survival rate of Chinese pine seedlings in the afforestation of the Chinese pine in the barren mountains is low, the afforestation and greening results in our province are seriously influenced, and particularly in recent years, the requirements of the construction of ecological provinces are more urgent to the greening requirements of our province. Therefore, the study on drought resistance and mechanism of the rivet mushroom fungus on the pine nursery stock and the application technology study of the rivet mushroom fungus in barren mountain forestation are studied.
The conclusion is as follows: the boletus can effectively promote the growth of the nursery stocks of the Chinese pine under the drought condition, and the growth of the nursery stocks of the Chinese pine is considered to mainly reduce the ratio of chlorophyll a to b and be beneficial to the drought resistance of plants; the membrane value peroxidation process can be effectively reduced, and the damage of drought stress to the cell membrane of the Chinese pine nursery stock can be reduced; can effectively generate antioxidant enzyme and prevent the damage of the adverse environment to plants.
The above-mentioned embodiments only express a certain implementation mode of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the present invention; it should be noted that, for those skilled in the art, without departing from the concept of the present invention, several variations and modifications can be made, which are within the protection scope of the present invention; therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (9)
1. The formula of the mycorrhizal bacterial fertilizer matrix of the rivet mushroom is characterized in that; comprises 500-800 g of the rivet mushroom strain, 20 jin of the cotton seed hull, 150 g of the compound fertilizer, 5 g of the mycorrhizal and 100 g of the talcum powder.
2. A production process of a mycorrhizal bacterial fertilizer matrix for rivet mushrooms is characterized by comprising the following steps:
s100, separating mycorrhizal fungi of the rivet mushroom fungi;
s200, culturing the separated mycorrhiza strains;
s300, propagating the cultured mycorrhizal strains;
s400, preparing a substrate;
s500, preparing a matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom.
3. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as claimed in claim 2, characterized in that: the step S200 of culturing the separated mycorrhizal fungi is to inoculate the separated mycorrhizal fungi onto a slant culture medium, group the slant culture medium into a group according to 5 test tubes, wrap the mouth of the test tube with sterile kraft paper, then tie the test tube with a rubber band, make an identification mark on the kraft paper, and then place all the test tube in a constant temperature and humidity incubator with 25 ℃ and 40% humidity to culture the fungi.
4. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as claimed in claim 2, characterized in that: in the step S300, the cultured mycorrhizal strain is inoculated again and cultured under the conditions of culturing in a constant temperature and humidity box, the temperature is adjusted to 25 ℃, and the humidity is adjusted to 60%.
5. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as claimed in claim 2, characterized in that: and in the step S300, the method further comprises the step of secondary strain propagation, wherein the cultured mycorrhizal strains are inoculated into 10 secondary culture bottles according to each test tube, the volume of each secondary culture bottle is 0.5 liter, the inoculation amount is about 1-3 g, the inoculation part is positioned at the center of each bottle, and when hyphae in the secondary culture bottles grow to be full, the hyphae are reserved for production and inoculation of the mycorrhizal fungi agents.
6. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as claimed in claim 2, characterized in that: the substrate in the step 400 is prepared by crushing the main raw materials of the substrate according to the formula of the substrate, fermenting for 15-20 hours, uniformly spraying water by using a spraying machine, uniformly stirring for later use, controlling the humidity of the substrate to be 50-60%, and filling the stirred substrate into a special material bag for sealing.
7. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as claimed in claim 2, characterized in that: the preparation of the mycorrhizal bacterial fertilizer matrix of the pleurotus ferulae in the step 500 is to inoculate the hyphae in the secondary culture bottle of the step S300 to the matrix in the special material bag after the hyphae grow full, so that the mycorrhizal bacterial fertilizer matrix of the pleurotus ferulae is obtained.
8. The production process of the mycorrhizal bacterial manure matrix of the rivet mushroom as recited in claim 7, characterized in that: controlling the pH value of the matrix of the mycorrhizal bacterial fertilizer of the rivet mushroom to be 5-6.
9. An application of a mycorrhizal bacterial fertilizer matrix of a rivet mushroom in promoting the growth direction of a Chinese pine seedling.
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Application publication date: 20201208 |