CN111956715A - Medicine for treating alcoholic liver disease and preparation method thereof - Google Patents
Medicine for treating alcoholic liver disease and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of medicine preparation, and discloses a medicine for treating alcoholic liver diseases and a preparation method thereof, wherein the medicine for treating alcoholic liver diseases comprises, by mass, 4-6 parts of codonopsis pilosula, 2-5 parts of pueraria lobata, 3-5 parts of poria alba, 2-3 parts of medicated leaven, 5-8 parts of coptis chinensis, 2-3 parts of rosa roxburghii tratt, 5-7 parts of rosa roxburghii leaves, 10-12 parts of green tea, 6-7 parts of bighead atractylodes rhizome, 2-3 parts of wolfberry fruit, 8-9 parts of hawthorn, 7-8 parts of salvia miltiorrhiza, 3-6 parts of spina date seeds and 4-7 parts of eucommia ulmoides. The traditional Chinese medicine composition has reasonable compatibility of medicinal materials, can eliminate damp and hot alcohol toxicity from top to bottom, can relieve the pain of alcoholic liver patients, and can effectively relieve and treat diseases; the medicine is mild and non-irritant, and can relieve the pain of patients in taking the medicine; has good effects of relieving alcoholism, protecting liver and reducing blood fat, and has remarkable curative effect on treating alcoholic liver disease. In addition, the preparation method of the medicine is simple, large-scale production can be realized, and the popularization is more convenient.
Description
Technical Field
The invention belongs to the technical field of medicine preparation, and particularly relates to a medicine for treating alcoholic liver diseases and a preparation method thereof.
Background
At present: the Alcoholic Liver Disease (ALD) is liver damage and a series of pathological changes caused by long-term large-volume drinking, and is clinically divided into five pathological changes with different degrees, namely, light ALD, alcoholic fatty liver, alcoholic hepatitis, alcoholic hepatic fibrosis and alcoholic cirrhosis according to an alcoholic liver disease diagnosis and treatment guideline revised by the Chinese medical society of hepatology and the alcoholic liver disease group in 1 month 2010. ALD is the leading cause of cirrhosis in western countries and is also one of the ten most common causes of death. In China, along with the continuous improvement of the living standard of people, the increase of social interaction, the increase of working pressure and the like, the number of people who drink wine also increases year by year. According to survey data, the incidence rate of ALD is on the increasing trend year by year in China, and the disease becomes the second largest liver disease after viral hepatitis, so that the prevention and treatment research on ALD has attracted great attention and attention in the same industry. Extensive and intensive research is carried out on the traditional Chinese medicine with the effect of resisting alcoholic liver injury, and the hope is that the action mechanism of the large compound prescription can be better researched and the anti-alcoholic liver injury medicine with better curative effect can be developed by determining the action mechanism of the traditional Chinese medicine components. However, no traditional Chinese medicine composition can realize effective treatment of alcoholic liver diseases at present, and no obvious effect exists in treatment by parting or special treatment and application of single-medicine active ingredient extract.
Through the above analysis, the problems and defects of the prior art are as follows: at present, no traditional Chinese medicine composition can realize effective treatment of alcoholic liver diseases, and no obvious effect exists in parting treatment or special treatment and application of single-medicine effective component extracts.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a medicine for treating alcoholic liver diseases and a preparation method thereof.
The invention is realized in such a way that the medicine for treating the alcoholic liver disease is composed of, by mass, 4-6 parts of codonopsis pilosula, 2-5 parts of pueraria lobata, 3-5 parts of poria alba, 2-3 parts of medicated leaven, 5-8 parts of coptis chinensis, 2-3 parts of rosa roxburghii tratt, 5-7 parts of rosa roxburghii tratt leaves, 10-12 parts of green tea, 6-7 parts of bighead atractylodes rhizome, 2-3 parts of wolfberry fruit, 8-9 parts of hawthorn, 7-8 parts of salvia miltiorrhiza, 3-6 parts of spina date seeds and 4-7 parts of eucommia ulmoides.
Another object of the present invention is to provide a method for preparing a drug for treating alcoholic liver disease, the method comprising the steps of:
step one, respectively removing impurities from codonopsis pilosula, pueraria flower, white poria, medicated leaven, coptis chinensis, rosa roxburghii tratt, green tea, bighead atractylodes rhizome, wolfberry fruit, hawthorn, salvia miltiorrhiza, spina date seed and eucommia ulmoides, cleaning, and then drying at room temperature;
step two, mixing codonopsis pilosula, spina date seeds, bighead atractylodes rhizome, salvia miltiorrhiza and eucommia ulmoides, putting the mixture into a marmite, adding water to immerse the medicinal materials for 12-18 hours, and decocting the medicinal materials with the immersion liquid; filtering after decoction is finished, and reserving filtrate for later use;
step three, adding the pueraria flower and the white poria into filter residues, adding water to submerge the medicinal materials, decocting again, filtering to obtain filtrate, and combining the filtrates obtained by decocting twice;
step four, standing the combined filtrate at room temperature, and removing the bottom residue precipitate; maintaining the temperature of the medicinal liquid at 60-70 deg.C, and concentrating under reduced pressure to obtain concentrated medicinal liquid;
step five, crushing the dried medicated leaven into particles with the particle size of below 300 mu m, and placing the particles into a jet mill for superfine crushing to obtain medicated leaven powder;
sixthly, drying and grinding the coptis chinensis, and sieving the coptis chinensis with a 100-mesh sieve to obtain coptis chinensis powder; mixing Coptidis rhizoma powder and fructus Rosae Normalis extract, and stirring to obtain fructus Rosae Normalis and Coptidis rhizoma slurry;
step seven, respectively adding 80-100 ℃ hot water into the medlar and the hawthorn to soak for 1-2 hours to obtain medlar soak solution and hawthorn soak solution;
step eight, drying and crushing fresh green tea to obtain tea powder, and adding water to extract green tea polyphenol;
mixing the concentrated liquid medicine, the medicated leaven powder and the roxburgh rose and coptis chinensis pulp, adding the medlar soak solution and the hawthorn soak solution, stirring, adjusting the temperature to 20-30 ℃, adding the green tea polyphenol, and uniformly stirring to obtain mixed pulp, namely the medicine for treating the alcoholic liver disease.
Further, in the second step, the decoction with the soak solution specifically comprises: heating at 80 deg.C for 10min, boiling the decoction, heating at 160 deg.C for 20min, and cooling to 760 deg.C for 2 hr.
Further, in the second step, a filtering method adopted during filtering is to filter by using 4 layers of gauze, wherein the gauze is 15-20 meshes.
Further, in the fourth step, when the liquid medicine is concentrated under reduced pressure, the concentration pressure is 2.5-3.2kPa, and the concentration time is 20 min.
Further, in the fifth step, the superfine grinding temperature is 18-25 ℃, the pressure is 1-2MPa when the material enters the superfine grinder, the superfine grinding pressure is 2-6MPa, the air flow rate is 220-260m/s, and the grinding time is 2-3 h.
Further, in the sixth step, when the coptis chinensis is dried, the drying temperature is 90-110 ℃, and the drying time is 40-50 min.
Further, in the sixth step, before the coptis chinensis is dried, the coptis chinensis is sliced and cut into slices with the thickness of 2-3 mm.
Further, in the sixth step, the preparation method of the roxburgh rose extract comprises the following steps:
(1) pulverizing Rosa roxburghii Tratt leaf, adding into water, leaching for 4-6 hr, and filtering to obtain Rosa roxburghii Tratt leaf water extractive solution and Rosa roxburghii Tratt leaf filter residue;
(2) adding the roxburgh rose leaf filter residue into ethanol, and performing reflux extraction to obtain roxburgh rose leaf extracting solution;
(3) mixing the roxburgh rose leaf water extract, the roxburgh rose leaf extract and roxburgh rose pulp, and concentrating to obtain a concentrated solution;
(4) dissolving sodium ascorbate in water, adding fructus Rosae Normalis blocks, and pulping in a pulping machine to obtain fructus Rosae Normalis pulp;
(5) mixing the concentrated solution with the fructus Rosae Normalis pulp to obtain fructus Rosae Normalis extract.
Further, the concentration of the concentrated solution is 1.0g/cm3-1.2g/cm3。
Further, in the eighth step, the preparation method of the green tea polyphenol comprises the following steps:
(1) drying fresh tea leaves until the water content is lower than 10%, crushing, and sieving with a 100-mesh sieve to obtain tea leaf powder;
(2) soaking tea powder in hot water of 70-80 deg.C for 30-50min, filtering to remove tea to obtain crude extractive solution;
(3) centrifuging the crude extract in a high-speed centrifuge for 10min to obtain centrifugate;
(4) filtering the centrifugate, and passing through an ultrafiltration membrane to obtain ultrafiltrate;
(5) adding chloroform into the ultrafiltrate, stirring, and separating to obtain tea polyphenol extract; recovering the reagent layer;
(6) circulating the tea polyphenol extract for 2 times, flowing through macroporous adsorption resin for adsorption, and eluting the resin with 20-60% ethyl acetate solution to obtain eluate;
(7) and decompressing the eluent, concentrating in vacuum, and drying to obtain the green tea polyphenol.
Further, when the resin is eluted, the temperature of the system is kept at 26-30 ℃, and the elution flow rate is 4.2-5.3 BV/h.
By combining all the technical schemes, the invention has the advantages and positive effects that: the codonopsis pilosula is sweet in taste and neutral in nature and enters spleen and lung meridians; has effects in invigorating spleen, replenishing qi, quenching thirst, invigorating spleen, replenishing lung qi, nourishing blood, and promoting fluid production, and can be used for treating deficiency of spleen-lung qi, anorexia, listlessness, cough, asthma, deficiency of qi and blood, sallow complexion, palpitation, short breath, thirst due to body fluid consumption, and diabetes due to internal heat. The flower of kudzuvine has sweet and cool taste; relieving hangover and activating spleen, and treating alcoholic injury, fever, polydipsia, anorexia, vomiting, acid regurgitation, hematemesis, and intestinal wind induced hemorrhage; treating dizziness, aversion to cold, high fever, hangover alleviating, spleen enlivening, dysentery, anorexia, chest distention, singultus, emesis, sour phlegm, alcohol toxicity damaging stomach, hematemesis, and heat removing. The white poria is sweet, light and neutral in nature; has the functions of promoting diuresis, eliminating dampness, invigorating spleen, regulating stomach function, calming heart and tranquilizing when used as a medicine; has effects in enhancing immunity, and has antitumor and liver protecting effects. Massa Medicata Fermentata has effects of dispelling food stagnation, invigorating spleen, promoting appetite, regulating stomach and relieving diarrhea. The coptis root clears heat and dries dampness, and purges fire and detoxifies; can be used for treating damp-heat distention, fullness, emesis, acid regurgitation, dysentery, jaundice, hyperpyrexia, coma, excessive heart-fire, vexation, insomnia, hematemesis, hemorrhage, conjunctival congestion, toothache, diabetes, carbuncle, furuncle, etc.; it is used externally to treat eczema, eczema and purulent ear canal. The wine coptis excels in clearing away heat in the upper-jiao; it can be used for treating conjunctival congestion and aphtha. Jiang Huang Lian can clear stomach, harmonize stomach and stop vomiting. Can be used for treating cold and heat accumulation, damp-heat obstruction, fullness and emesis. YUHUANGLIAN has effects in soothing liver, regulating stomach function, and relieving vomit; can be used for treating liver and stomach disharmony, emesis and acid regurgitation. Bighead atractylodes rhizome, rhizoma Atractylodis Macrocephalae, with its medicinal function of activating the spleen, is bitter, warm, pungent and strong in property and taste, and has the actions of drying dampness, resolving turbidity and relieving pain. Wolfberry fruit, sweet and neutral; wolfberry fruit, fructus Lycii, for nourishing liver, nourishing kidney and moistening lung. The dried hawthorn can be used as a medicine, has the effects of reducing blood fat and blood pressure, strengthening heart, resisting arrhythmia and the like, is also a good medicine for strengthening spleen and stimulating appetite, promoting digestion and removing food stagnation, and promoting blood circulation and reducing phlegm, and has good curative effects on chest, diaphragm and spleen fullness, hernia, blood stasis, amenorrhea and other symptoms. Saviae Miltiorrhizae radix contains tanshinone, is a tonic and meridian dredging agent, and has effects of removing blood stasis, promoting tissue regeneration, promoting blood circulation, and regulating menstruation; in addition, the medicine also can be used for treating insomnia caused by neurasthenia, arthralgia, anemia, mastitis, lymphadenitis, arthritis, sore and furuncle pain and swelling, erysipelas, acute and chronic hepatitis, kidney menyan nephritis, traumatic injury, liver and spleen swelling caused by late schistosomiasis, and epilepsy 30286. The semen Ziziphi Spinosae contains heavy fatty oil and protein, and contains sterol, triterpenes, semen Ziziphi Spinosae saponin, and vitamin C; has tranquilizing, hypnotic, analgesic, and anticonvulsive effects; has certain blood pressure lowering effect; used for treating yin-blood deficiency, palpitation, insomnia and amnesia; asthenia and hyperhidrosis. Eucommia bark is sweet in taste and warm in nature. Has effects in tonifying liver and kidney, strengthening muscle and bone, regulating Chong and ren meridians, and preventing miscarriage; can be used for treating lumbago, leg pain or soreness and weakness due to kidney yang deficiency, and infertility due to liver qi deficiency.
The traditional Chinese medicine composition has reasonable compatibility of medicinal materials, can eliminate damp and hot alcohol toxicity from top to bottom, can relieve the pain of alcoholic liver patients, and can effectively relieve and treat diseases; the medicine is mild and non-irritant, and can relieve the pain of patients in taking the medicine; has good effects of relieving alcoholism, protecting liver and reducing blood fat, and has remarkable curative effect on treating alcoholic liver disease. In addition, the preparation method of the medicine is simple, large-scale production can be realized, and the popularization is more convenient.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
Fig. 1 is a flowchart of a method for preparing a drug for treating alcoholic liver disease according to an embodiment of the present invention.
Fig. 2 is a flow chart of a preparation method of a roxburgh rose extract provided by the embodiment of the invention.
Fig. 3 is a flowchart of a method for preparing green tea polyphenols according to an embodiment of the present invention.
FIG. 4 is a graph comparing liver function indices of rats provided in the examples of the present invention.
FIG. 5 is a morphological diagram of hepatocytes according to an embodiment of the present invention, FIG. 5(a) is a morphological diagram of rat hepatocytes according to example 2, and FIG. 5(b) is a morphological diagram of rat hepatocytes according to example 3.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a medicament for treating alcoholic liver diseases and a preparation method thereof, and the invention is described in detail with reference to the accompanying drawings.
As shown in fig. 1, the preparation method of the drug for treating alcoholic liver disease provided by the embodiment of the invention includes the following steps:
s101, respectively removing impurities from codonopsis pilosula, pueraria flower, white poria, medicated leaven, coptis chinensis, rosa roxburghii tratt, green tea, bighead atractylodes rhizome, wolfberry fruit, hawthorn, salvia miltiorrhiza, spina date seed and eucommia ulmoides, cleaning, and drying at room temperature;
s102, mixing radix codonopsitis, spina date seed, bighead atractylodes rhizome, salvia miltiorrhiza and eucommia bark, putting the mixture into a marmite, adding water to immerse the medicinal materials for 12-18 hours, and decocting the medicinal materials with the immersion liquid; filtering after decoction is finished, and reserving filtrate for later use;
s103, adding the pueraria flower and the white poria into filter residues, adding water to immerse the medicinal materials, decocting again, filtering to obtain filtrate, and combining the filtrates obtained by decocting twice;
s104, standing the combined filtrate at room temperature, and removing the bottom residue precipitate; maintaining the temperature of the medicinal liquid at 60-70 deg.C, and concentrating under reduced pressure to obtain concentrated medicinal liquid;
s105, crushing the dried medicated leaven into particles with the particle size of below 300 mu m, and placing the particles into a jet mill for superfine crushing to obtain medicated leaven powder;
s106, drying and grinding the coptis chinensis, and sieving the coptis chinensis with a 100-mesh sieve to obtain coptis chinensis powder; mixing Coptidis rhizoma powder and fructus Rosae Normalis extract, and stirring to obtain fructus Rosae Normalis and Coptidis rhizoma slurry;
s107, respectively adding 80-100 ℃ hot water into the wolfberry fruits and the hawthorns for soaking for 1-2 hours to obtain wolfberry fruit soaking solutions and hawthorn fruit soaking solutions;
s108, drying and crushing fresh green tea to obtain tea powder, and adding water to extract green tea polyphenol;
s109, mixing the concentrated liquid medicine, the medicated leaven powder and the roxburgh rose and coptis chinensis pulp, adding the wolfberry fruit soaking solution and the hawthorn fruit soaking solution, stirring, adjusting the temperature to 20-30 ℃, adding the green tea polyphenol, and uniformly stirring to obtain mixed pulp, namely the medicine for treating the alcoholic liver disease.
In step S102 of the embodiment of the present invention, the decocting with the soak solution specifically includes: heating at 80 deg.C for 10min, boiling the decoction, heating at 160 deg.C for 20min, and cooling to 760 deg.C for 2 hr.
In step S102 of the embodiment of the present invention, a filtering method adopted during filtering is to filter by using 4 layers of gauze, where the gauze is 15-20 meshes.
In step S104 of the embodiment of the present invention, when the liquid chemical is concentrated under reduced pressure, the concentration pressure is 2.5 to 3.2kPa, and the concentration time is 20 min.
In step S105 of the embodiment of the invention, the superfine grinding temperature is 18-25 ℃, the pressure is 1-2MPa when the material enters the superfine grinder, the superfine grinding pressure is 2-6MPa, the air flow rate is 220-260m/S, and the grinding time is 2-3 h.
In step S106 of the embodiment of the invention, when the coptis chinensis is dried, the drying temperature is 90-110 ℃, and the drying time is 40-50 min.
In step S106 of the embodiment of the present invention, before drying the coptis chinensis, the coptis chinensis is sliced and cut into pieces with a thickness of 2 to 3 mm.
As shown in fig. 2, in step S106, the preparation method of the rosa roxburghii tratt extract provided by the embodiment of the invention includes the following steps:
s201, crushing roxburgh rose leaves, adding the roxburgh rose leaves into water, leaching for 4-6 hours, and filtering to obtain roxburgh rose leaf water leaching solution and roxburgh rose leaf filter residues;
s202, adding the roxburgh rose leaf filter residue into ethanol, and performing reflux extraction to obtain roxburgh rose leaf extracting solution;
s203, mixing the roxburgh rose leaf water extract, the roxburgh rose leaf extract and roxburgh rose pulp, and concentrating to obtain a concentrated solution;
s204, dissolving sodium ascorbate in water, adding the roxburgh rose blocks, and adding the roxburgh rose blocks into a pulping machine for pulping to obtain roxburgh rose pulp;
s205, mixing the concentrated solution into the roxburgh rose pulp to obtain the roxburgh rose extract.
The concentration of the concentrated solution provided by the embodiment of the invention is 1.0g/cm3-1.2g/cm3。
As shown in fig. 3, in step S108, the method for preparing green tea polyphenol according to the embodiment of the present invention includes:
s301, drying fresh tea leaves until the water content is lower than 10%, crushing, and sieving with a 100-mesh sieve to obtain tea leaf powder;
s302, soaking the tea powder in hot water at 70-80 ℃ for 30-50min, and filtering to remove tea leaves to obtain a crude extract;
s303, placing the crude extract in a high-speed centrifuge for centrifugation for 10min to obtain a centrifugate;
s304, filtering the centrifugate, and passing through an ultrafiltration membrane to obtain ultrafiltrate;
s305, adding chloroform into the ultrafiltrate, stirring, and separating liquid to obtain a tea polyphenol extract liquid; recovering the reagent layer;
s306, circulating the tea polyphenol extract for 2 times, flowing through macroporous adsorption resin for adsorption, and eluting the resin by using ethyl acetate solution with the concentration of 20-60% to obtain eluent;
s307, decompressing the eluent, concentrating in vacuum, and drying to obtain the green tea polyphenol.
When the resin provided by the embodiment of the invention is eluted, the temperature of the system is kept at 26-30 ℃, and the elution flow rate is 4.2-5.3 BV/h.
The medicine for treating alcoholic liver diseases provided by the embodiment of the invention is composed of, by mass, 4-6 parts of codonopsis pilosula, 2-5 parts of flos puerariae lobatae, 3-5 parts of poria alba, 2-3 parts of medicated leaven, 5-8 parts of coptis chinensis, 2-3 parts of rosa roxburghii tratt, 5-7 parts of rosa roxburghii tratt leaves, 10-12 parts of green tea, 6-7 parts of bighead atractylodes rhizome, 2-3 parts of wolfberry fruit, 8-9 parts of hawthorn, 7-8 parts of salvia miltiorrhiza, 3-6 parts of spina date seeds and 4-7 parts of eucommia ulmoides.
The technical solution of the present invention is further described with reference to the following specific examples.
Example 1:
case one:
wangzhi, male, age 48, alcoholism, history of alcoholic liver disease, hospitalization before one week due to epigastric distending pain; when the medicine is used for treatment, the abdominal pain is relieved after two days, and no obvious pain is caused by light pressure; the medicine is discharged and treated by taking the medicine.
Case 2:
zhangqi, male, age 31, with alcoholic liver disease, delays obvious pain; the medicine is used for treatment, wine is strictly prohibited during the medicine taking period, and the face color is obviously improved and the energy is better after the medicine is taken for a year; the rechecking result shows that the alcoholic liver disease is effectively improved.
Example 2:
removing impurities from radix Codonopsis, flos Puerariae Lobatae, Poria, Massa Medicata Fermentata, Coptidis rhizoma, fructus Rosae Normalis, green tea, Atractylodis rhizoma, fructus Lycii, fructus crataegi, Saviae Miltiorrhizae radix, semen Ziziphi Spinosae and Eucommiae cortex, cleaning, and drying at room temperature; mixing the above materials, placing in casserole, adding water to submerge the materials, soaking for 12-18h, and decocting with the soaking solution; filtering after decoction is finished, and reserving filtrate for later use; decocting the residue with water, filtering to obtain filtrate, and mixing the two decoctions; standing the combined filtrate at room temperature, and removing the bottom residue precipitate; maintaining the temperature of the medicinal liquid at 60-70 deg.C, and concentrating under reduced pressure to obtain concentrated medicinal liquid.
Example 3:
removing impurities from radix Codonopsis, flos Puerariae Lobatae, Poria, Massa Medicata Fermentata, Coptidis rhizoma, fructus Rosae Normalis, green tea, Atractylodis rhizoma, fructus Lycii, fructus crataegi, Saviae Miltiorrhizae radix, semen Ziziphi Spinosae and Eucommiae cortex, cleaning, and drying at room temperature; mixing radix Codonopsis, semen Ziziphi Spinosae, Atractylodis rhizoma, Saviae Miltiorrhizae radix, and Eucommiae cortex, placing in casserole, adding water to submerge the medicinal materials, soaking for 12-18h, and decocting with the soaking solution; filtering after decoction is finished, and reserving filtrate for later use; adding flos Puerariae Lobatae and Poria into the residue, adding water, decocting, filtering to obtain filtrate, and mixing the two decoctions; standing the combined filtrate at room temperature, and removing the bottom residue precipitate; maintaining the temperature of the medicinal liquid at 60-70 deg.C, and concentrating under reduced pressure to obtain concentrated medicinal liquid;
pulverizing dried Massa Medicata Fermentata to particle size below 300 μm, and micronizing in jet mill to obtain Massa Medicata Fermentata powder; drying the coptis chinensis, grinding, and sieving by a 100-mesh sieve to obtain coptis chinensis powder; mixing Coptidis rhizoma powder and fructus Rosae Normalis extract, and stirring to obtain fructus Rosae Normalis and Coptidis rhizoma slurry; respectively soaking fructus Lycii and fructus crataegi in 80-100 deg.C hot water for 1-2 hr to obtain fructus Lycii soaking solution and fructus crataegi soaking solution; drying fresh green tea, pulverizing to obtain tea powder, and extracting with water to obtain green tea polyphenol;
mixing the concentrated liquid medicine, Massa Medicata Fermentata powder and fructus Rosae Normalis and Coptidis rhizoma slurry, adding fructus Lycii soaking solution and fructus crataegi soaking solution, stirring, adjusting temperature to 20-30 deg.C, adding green tea polyphenols, and stirring to obtain mixed slurry, i.e. medicine for treating alcoholic liver disease.
Example 4:
an alcoholic liver disease animal model was established, and the drug efficacy of the drugs for treating alcoholic liver disease prepared in example 2 and example 3 was measured:
dividing 50 rats with alcoholic liver diseases into 2 groups, namely a control group and an experimental group, respectively, performing intragastric administration on the control group with the drug for treating the alcoholic liver diseases in the embodiment 2, and performing intragastric administration on the experimental group with the drug for treating the alcoholic liver diseases in the embodiment 3; the experiment was performed for 30 weeks, all rats were sacrificed at one time using femoral bleeding, and serum was collected and isolated from each rat and labeled.
Liver function index of each group of rats is measured by a full-automatic biochemical analyzer, and the measurement result is shown in figure 4;
the morphology of the hepatocytes was examined, and FIG. 5 is a diagram of the morphology of the hepatocytes.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. The preparation method of the medicine for treating the alcoholic liver disease is characterized by comprising the following steps of:
step one, respectively removing impurities from codonopsis pilosula, pueraria flower, white poria, medicated leaven, coptis chinensis, rosa roxburghii tratt, green tea, bighead atractylodes rhizome, wolfberry fruit, hawthorn, salvia miltiorrhiza, spina date seed and eucommia ulmoides, cleaning, and then drying at room temperature;
step two, mixing codonopsis pilosula, spina date seeds, bighead atractylodes rhizome, salvia miltiorrhiza and eucommia ulmoides, putting the mixture into a marmite, adding water to immerse the medicinal materials for 12-18 hours, and decocting the medicinal materials with the immersion liquid; filtering after decoction is finished, and reserving filtrate for later use;
step three, adding the pueraria flower and the white poria into filter residues, adding water to submerge the medicinal materials, decocting again, filtering to obtain filtrate, and combining the filtrates obtained by decocting twice;
step four, standing the combined filtrate at room temperature, and removing the bottom residue precipitate; maintaining the temperature of the medicinal liquid at 60-70 deg.C, and concentrating under reduced pressure to obtain concentrated medicinal liquid;
step five, crushing the dried medicated leaven into particles with the particle size of below 300 mu m, and placing the particles into a jet mill for superfine crushing to obtain medicated leaven powder;
when the superfine grinding is carried out, the grinding temperature is 18-25 ℃, the pressure is 1-2Mpa when the superfine grinding machine enters, the superfine grinding pressure is 2-6Mpa, the air flow speed is 220-260m/s, and the grinding time is 2-3 h;
sixthly, drying the coptis chinensis at the temperature of 90-110 ℃ for 40-50min, grinding after drying, and sieving with a 100-mesh sieve to obtain coptis chinensis powder; mixing Coptidis rhizoma powder and fructus Rosae Normalis extract, and stirring to obtain fructus Rosae Normalis and Coptidis rhizoma slurry;
step seven, respectively adding 80-100 ℃ hot water into the medlar and the hawthorn to soak for 1-2 hours to obtain medlar soak solution and hawthorn soak solution;
step eight, drying and crushing fresh green tea to obtain tea powder, and adding water to extract green tea polyphenol;
mixing the concentrated liquid medicine, the medicated leaven powder and the roxburgh rose and coptis chinensis pulp, adding the medlar soak solution and the hawthorn soak solution, stirring, adjusting the temperature to 20-30 ℃, adding the green tea polyphenol, and uniformly stirring to obtain mixed pulp, namely the medicine for treating the alcoholic liver disease.
2. The method for preparing a medicament for treating alcoholic liver disease as claimed in claim 1, wherein the step two, the decoction with the soak solution specifically includes: heating at 80 deg.C for 10min, boiling the decoction, heating at 160 deg.C for 20min, and cooling to 760 deg.C for 2 hr.
3. The method for preparing a medicament for treating alcoholic liver disease of claim 1, wherein in the second step, the filtering method is performed by using 4 layers of gauze, and the gauze is 15-20 meshes.
4. The method for preparing a pharmaceutical preparation for treating alcoholic liver disease of claim 1, wherein in the fourth step, when the liquid medicine is concentrated under reduced pressure, the concentration pressure is 2.5-3.2kPa, and the concentration time is 20 min.
5. The method of claim 1, wherein in step six, the coptis chinensis is sliced and cut into pieces 2-3mm thick before being dried.
6. The method for preparing a medicament for treating alcoholic liver disease as claimed in claim 1, wherein in the sixth step, the method for preparing the rosa roxburghii tratt extract comprises the following steps:
(1) pulverizing Rosa roxburghii Tratt leaf, adding into water, leaching for 4-6 hr, and filtering to obtain Rosa roxburghii Tratt leaf water extractive solution and Rosa roxburghii Tratt leaf filter residue;
(2) adding the roxburgh rose leaf filter residue into ethanol, and performing reflux extraction to obtain roxburgh rose leaf extracting solution;
(3) mixing the roxburgh rose leaf water extract, the roxburgh rose leaf extract and roxburgh rose pulp, and concentrating to obtain a concentrated solution;
(4) dissolving sodium ascorbate in water, adding fructus Rosae Normalis blocks, and pulping in a pulping machine to obtain fructus Rosae Normalis pulp;
(5) mixing the concentrated solution with the fructus Rosae Normalis pulp to obtain fructus Rosae Normalis extract.
7. The method of claim 6, wherein the concentration of the concentrate is 1.0g/cm3-1.2g/cm3。
8. The method for preparing a medicament for treating alcoholic liver disease as claimed in claim 1, wherein in step eight, the method for preparing green tea polyphenol comprises:
(1) drying fresh tea leaves until the water content is lower than 10%, crushing, and sieving with a 100-mesh sieve to obtain tea leaf powder;
(2) soaking tea powder in hot water of 70-80 deg.C for 30-50min, filtering to remove tea to obtain crude extractive solution;
(3) centrifuging the crude extract in a high-speed centrifuge for 10min to obtain centrifugate;
(4) filtering the centrifugate, and passing through an ultrafiltration membrane to obtain ultrafiltrate;
(5) adding chloroform into the ultrafiltrate, stirring, and separating to obtain tea polyphenol extract; recovering the reagent layer;
(6) circulating the tea polyphenol extract for 2 times, flowing through macroporous adsorption resin for adsorption, and eluting the resin with 20-60% ethyl acetate solution to obtain eluate;
(7) and decompressing the eluent, concentrating in vacuum, and drying to obtain the green tea polyphenol.
9. The method for preparing a medicament for treating alcoholic liver disease of claim 8, wherein the resin is eluted while maintaining the system temperature at 26-30 ℃ and the elution flow rate at 4.2-5.3 BV/h.
10. The medicine for treating the alcoholic liver disease, which is prepared by the preparation method of the medicine for treating the alcoholic liver disease as claimed in any one of claims 1 to 9, is characterized by comprising, by mass, 4-6 parts of codonopsis pilosula, 2-5 parts of pueraria lobata, 3-5 parts of poria alba, 2-3 parts of medicated leaven, 5-8 parts of coptis chinensis, 2-3 parts of rosa roxburghii tratt, 5-7 parts of rosa roxburghii tratt leaf, 10-12 parts of green tea, 6-7 parts of bighead atractylodes rhizome, 2-3 parts of wolfberry fruit, 8-9 parts of hawthorn, 7-8 parts of salvia miltiorrhiza, 3-6 parts of spina date seed and 4-7 parts of eucommia ulmoides.
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