CN111849708A

CN111849708A - High-efficient culture medium injection device

Info

Publication number: CN111849708A
Application number: CN202010763869.7A
Authority: CN
Inventors: 蔡文楠; 常炜; 邹士尧; 张玉臣; 李玉巍; 刘莉颖; 郑虹; 何存玲
Original assignee: Qinghai Provincial Drug Inspection And Testing Institute
Current assignee: Qinghai Provincial Drug Inspection And Testing Institute
Priority date: 2020-08-01
Filing date: 2020-08-01
Publication date: 2020-10-30

Abstract

The invention discloses an efficient culture medium injection device, and belongs to the technical field of injection devices. The utility model provides a high-efficient culture medium injection apparatus, includes the box, one side fixedly connected with fixed plate in the box, fixedly connected with injection tube on the fixed plate, sliding connection has the clamp plate in the injection tube, the liquid outlet of injection tube is connected with out the liquid tubule, the lower extreme rotates through the pivot in the box and is connected with first toothed disc, through the setting of first motor, second toothed disc, messenger's device has realized carrying out the characteristics of injecting to the culture container automatically in the invention, has significantly reduced researcher's the amount of labour, has improved work efficiency, through the setting of first closing plate, second closing plate, the effectual backward flow that prevents the culture medium, has realized the ration injection to the culture medium in the culture container, has improved the device's practicality, and has further satisfied user's in-service use demand.

Description

High-efficient culture medium injection device

Technical Field

The invention relates to the technical field of injection devices, in particular to a high-efficiency culture medium injection device.

Background

The culture medium is a nutrient substrate prepared by combining different nutrient substances for the places where microorganisms, animals, plants or tissues grow and propagate, generally contains several substances such as carbohydrates, nitrogen-containing substances, inorganic salt vitamins, water and the like, and is a basic substance for providing cell nutrition and promoting cell proliferation as well as a living environment for cell growth and propagation.

However, in some traditional injection means, generally, the laboratory staff holds the injection tube to carry out the injection of culture medium to it, and the laboratory staff needs to inject a plurality of culture containers that are equipped with the microorganism, has improved laboratory staff's intensity of labour greatly, has reduced work efficiency, though some injection apparatus of current have replaced artifical injection, but when injecting, the tray under the culture container can not carry out run-in step, and the practicality is lower, can not satisfy actual work demand, consequently, an urgent need for can carry out the high-efficient culture medium injection apparatus of injection to culture container automatically.

Disclosure of Invention

The invention aims to solve the problems that in the traditional injection means, an experimenter generally holds an injection tube to inject culture medium, the experimenter needs to inject a plurality of culture containers filled with microorganisms, the labor intensity of the experimenter is greatly improved, and the working efficiency is reduced.

In order to achieve the purpose, the invention adopts the following technical scheme:

a high-efficiency culture medium injection device comprises a box body, wherein a fixed plate is fixedly connected to one side in the box body, an injection tube is fixedly connected to the fixed plate, a pressing plate is connected to the injection tube in a sliding manner, air vents are formed in two sides of the upper end of the injection tube, a liquid outlet of the injection tube is connected with liquid outlet tubules, a hose is connected to a liquid inlet of the injection tube, a first gear disc is rotatably connected to the lower end in the box body through a rotating shaft, a plurality of ware grooves are formed in the first gear disc, the liquid outlet tubules are matched with the ware grooves in position, a first motor is fixedly connected to one end, far away from the fixed plate, of the upper end in the box body, a first fixed rod is fixedly connected to one side, close to the first motor, of the box body, a, and is fixedly connected with a crank, one end of the crank, which is far away from the first rotating rod, is rotatably connected with a connecting rod, one end of the connecting rod, which is far away from the crank, is rotatably connected with a movable rod, one end of the movable rod, which is far away from the connecting rod, is fixedly connected with a pressing plate, the lower end of one side of the fixed plate is fixedly connected with a fixed block, the fixed block is rotatably connected with a second rotating rod, the second rotating rod is fixedly connected with a driven wheel, the first rotating rod is fixedly connected with a driving wheel matched with the driven wheel in position, the driving wheel is connected with the driven wheel through a belt, one end of the second rotating rod, which is far away from the fixed block, is fixedly connected with a first bevel gear, the lower end, which is close to the first motor, in the, third dwang upper end fixedly connected with second bevel gear, mesh between first bevel gear and the second bevel gear mutually, third dwang lower extreme fixedly connected with and first gear dish engaged with second gear dish.

Preferably, be connected through thread sealing rotation between play tubule and the injection tube, play tubule inner wall both sides fixedly connected with backup pad, the first sleeve of fixedly connected with in the backup pad, sliding connection has first removal post in the first sleeve, the inlet of play tubule is equipped with first closing plate, two the first removal post is kept away from the both sides of first telescopic one end difference fixed connection at first closing plate, first return spring has been cup jointed on the first removal post, the both ends of first return spring respectively with first closing plate, first sleeve fixed connection.

Preferably, through connecting thread sealing connection between injection tube and the hose, the inlet both sides fixedly connected with loading board of injection tube, fixedly connected with second sleeve on the loading board, sliding connection has the second to remove the post in the second sleeve, the inlet of injection tube is equipped with the second closing plate, two the second removes the one end that the second was kept away from to the post and is fixed connection respectively in the both sides of second closing plate, the second removes and has cup jointed second return spring on the post, second sleeve, second closing plate fixed connection are distinguished to the both ends of second return spring.

Preferably, the first sealing plate and the second sealing plate are both provided with rubber cushions.

Preferably, the surfaces of the joint and the liquid outlet tubule are provided with anti-skid lines.

Preferably, the number of grooves formed in the lower end of the interior of the box body is 4, the grooves are internally connected with balls in a rolling mode, and the balls are connected with the first gear plate in a rolling mode.

Preferably, the number of the vessel grooves fixedly connected to the first gear plate is 6.

Preferably, one side of the box body is rotatably connected with a box door through a hinge, and the box door is a transparent plate.

Preferably, one side of the lower end of the fixing plate is fixedly connected with an illuminating lamp, and the illuminating lamp is electrically connected with an external power supply.

Preferably, soundproof cotton is arranged on the surface of the first motor.

Compared with the prior art, the invention provides a high-efficiency culture medium injection device, which has the following beneficial effects:

1. the high-efficiency culture medium injection device is characterized in that when a user uses the device, the device is firstly placed on a specified workbench, the inner lower end of a box body is rotatably connected with a first gear disc through a rotating shaft, a plurality of utensil grooves are formed in the first gear disc, the user firstly places a culture container in the utensil grooves to fix the culture container, the culture container is prevented from displacing due to vibration and affecting the injection effect, at the moment, the user starts a first motor to rotate a first rotating rod and further drive a crank to rotate, a connecting rod is connected to the crank, a moving rod is connected to the connecting rod and connected with the moving rod, the moving rod is connected with a pressing plate to drive the pressing plate to move up and down, a driving wheel is connected to the first rotating rod, the driving wheel is connected with a driven wheel through a belt and is fixed to a second rotating rod, one end of the second rotating rod is connected with, the third rotating rod is connected with a second bevel gear meshed with the first bevel gear, so that the third rotating rod is driven to rotate through the first rotating rod, the third rotating rod is connected with a second gear disc meshed with the first gear disc, the first gear disc is further driven to rotate, a container filled with a culture medium is connected with the injection tube through a hose by a user, and the device is used for injecting a culture container filled with microorganisms by starting the first motor;

the device has the advantages that the device has the characteristic of automatically injecting the culture container through the arrangement of the first motor and the second gear wheel disc, the labor capacity of researchers is greatly reduced, the working efficiency is improved, the backflow of the culture medium is effectively prevented through the arrangement of the first sealing plate and the second sealing plate, the quantitative injection of the culture medium in the culture container is realized, the practicability of the device is improved, and the actual use requirements of users are further met.

Drawings

FIG. 1 is a schematic view of an efficient medium injection device according to the present invention;

FIG. 2 is a left side view of a high efficiency media injection apparatus according to the present invention;

FIG. 3 is a second schematic view of the structure of a high efficiency medium injection device according to the present invention;

FIG. 4 is a third schematic view of an efficient medium injection device according to the present invention;

FIG. 5 is a schematic view showing the structure of part A of FIG. 3 of a highly efficient medium injection apparatus according to the present invention;

FIG. 6 is a schematic view showing the structure of part B of FIG. 4 of a highly efficient medium injection apparatus according to the present invention;

FIG. 7 is a fourth schematic view of the structure of an efficient culture medium injection device according to the present invention;

FIG. 8 is a fifth schematic view showing the structure of an efficient medium injection device according to the present invention.

In the figure: 1. a box body; 2. a box door; 3. a first motor; 4. an injection tube; 5. a fixing plate; 6. a first rotating lever; 7. a driving wheel; 8. a crank; 9. a connecting rod; 10. a travel bar; 11. pressing a plate; 12. a first fixing lever; 13. a liquid outlet thin tube; 14. a vessel trough; 15. a fixed block; 16. a second rotating lever; 17. a first bevel gear; 18. a second bevel gear; 19. a third rotating rod; 20. a second fixing bar; 21. a first gear plate; 22. a ball bearing; 23. a joint; 24. a hose; 25. a carrier plate; 26. a second sealing plate; 27. a support plate; 28. a first sealing plate; 29. a first sleeve; 30. a first moving column; 31. a first return spring; 32. a second sleeve; 33. a second moving column; 34. a second return spring; 101. a second gear wheel disc; 102. An ultraviolet lamp; 401. and (4) a vent hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Example 1:

referring to fig. 1-8, a high-efficiency culture medium injection device comprises a box body 1, wherein a fixed plate 5 is fixedly connected to one side in the box body 1, an injection tube 4 is fixedly connected to the fixed plate 5, a pressing plate 11 is slidably connected to the injection tube 4, vent holes 401 are formed in two sides of the upper end of the injection tube 4, a liquid outlet of the injection tube 4 is connected with a liquid outlet tubule 13, a liquid inlet of the injection tube 4 is connected with a hose 24, a first gear disc 21 is rotatably connected to the inner lower end of the box body 1 through a rotating shaft, a plurality of vessel grooves 14 are formed in the first gear disc 21, the liquid outlet tubule 13 is matched with the vessel grooves 14 in position, a first motor 3 is fixedly connected to one end of the inner upper end of the box body 1, which is far away from the fixed plate 5, a first fixing rod 12 is fixedly connected to one side, which, the one end that first motor 3 was kept away from to first dwang 6 runs through in first dead lever 12, and fixedly connected with crank 8, the one end that first dwang 6 was kept away from to crank 8 is rotated and is connected with connecting rod 9, the one end that crank 8 was kept away from to connecting rod 9 is rotated and is connected with carriage release lever 10, the one end that connecting rod 9 was kept away from to carriage release lever 10 and clamp plate 11 fixed connection, fixed plate 5 one side lower extreme fixedly connected with fixed block 15, rotate on fixed block 15 and be connected with second dwang 16, fixedly connected with from the driving wheel on the second dwang 16, fixedly connected with on the first dwang 6 with from driving wheel 7 of driving wheel position phase-match, driving wheel 7 with from being connected through the belt between the driving wheel, the one end fixedly connected with first conical gear 17 that fixed block 15 was kept away from to second dwang 16, the lower extreme fixedly connected with, the last rotation of second dead lever 20 is connected with third dwang 19, 19 lower extremes of third dwang are rotated through the pivot with 1 bottom of box and are connected, 19 upper end fixedly connected with second bevel gear 18 of third dwang, mesh mutually between first bevel gear 17 and the second bevel gear 18, 19 lower extreme fixedly connected with of third dwang and first gear dish 21 second gear dish 101 of meshing mutually.

When the device is used by a user, the device is firstly placed on a specified workbench, wherein the lower end in the box body 1 is rotatably connected with a first gear disc 21 through a rotating shaft, a plurality of vessel grooves 14 are arranged on the first gear disc 21, the user firstly places a culture vessel in the vessel grooves 14 for fixing the culture vessel and preventing the culture vessel from displacing due to vibration to influence the injection effect, at the moment, the user starts the first motor 3 to rotate the first rotating rod 6 and further drive the crank 8 to rotate, the crank 8 is connected with a connecting rod 9, the connecting rod 9 is connected with a moving rod 10, the moving rod 10 is connected with the pressing plate 11 and further drive the pressing plate 11 to move up and down, the first rotating rod 6 is connected with a driving wheel 7, the driving wheel 7 is connected with a driven wheel through a belt, the driven wheel is fixed on a second rotating rod 16, one end of the second rotating rod 16 is connected, 1 bottom of box rotates and is connected with third dwang 19, be connected with the second bevel gear 18 with first bevel gear 17 engaged with on the third dwang 19, thereby it rotates to drive third dwang 19 through first dwang 6, be connected with the second fluted disc 101 with first fluted disc 21 engaged with on the third dwang 19, and then drive first fluted disc 21 and rotate, the user passes through hose 24 and injection tube 4 through the container that the culture medium will be equipped with to the user this moment and is connected, make the device inject the culture container that contains the microorganism through starting first motor 3, the device has realized can carry out the characteristics of injection to the culture container automatically, researcher's the amount of labour has significantly reduced, work efficiency is improved, and then actual work demand has been satisfied.

Example 2:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the liquid outlet tubule 13 is rotatably connected with the injection tube 4 through thread sealing, two sides of the inner wall of the liquid outlet tubule 13 are fixedly connected with a support plate 27, the support plate 27 is fixedly connected with a first sleeve 29, a first moving column 30 is slidably connected in the first sleeve 29, a first sealing plate 28 is arranged at the liquid inlet of the liquid outlet tubule 13, one end of each of the two first moving columns 30 far away from the first sleeve 29 is respectively and fixedly connected with two sides of the first sealing plate 28, a first return spring 31 is sleeved on the first moving column 30, two ends of the first return spring 31 are respectively and fixedly connected with the first sealing plate 28 and the first sleeve 29, the liquid outlet tubule 13 is connected with the injection tube 4 through threads, a user can conveniently clean and replace the liquid outlet tubule 13, the practicability of the device is improved, through the arrangement of the first sealing plate 28, when the pressing plate 11 moves upwards, the first sealing plate 28 can block, prevent the backflow of the culture medium and influence the injection amount of the culture medium in the culture container.

Example 3:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the injection tube 4 is connected with the hose 24 through a connector 23 in a threaded sealing manner, the two sides of the liquid inlet of the injection tube 4 are fixedly connected with a bearing plate 25, a second sleeve 32 is fixedly connected on the bearing plate 25, a second moving column 33 is connected in the second sleeve 32 in a sliding manner, a second sealing plate 26 is arranged at the liquid inlet of the injection tube 4, one ends of the two second moving columns 33 far away from the second sleeve 32 are respectively and fixedly connected with the two sides of the second sealing plate 26, a second return spring 34 is sleeved on the second moving column 33, the two ends of the second return spring 34 are respectively and fixedly connected with the second sleeve 32 and the second sealing plate 26, through the arrangement of the connector 23, a user can conveniently clean and replace the hose 24, the practicability of the device is further improved, through the arrangement of the second sealing plate 26, when the pressing plate 11 moves upwards, the second sealing plate 26 is opened under the action of negative pressure, and then the culture medium, when the pressing plate 11 moves down, the second sealing plate 26 will block the liquid inlet of the injection tube 4, so that the culture medium can not enter the injection tube 4, and the quantitative injection of the microorganism in the culture container is realized, thereby meeting the actual use requirements of users.

Example 4:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the first sealing plate 28 and the second sealing plate 26 are both provided with rubber cushions, so that the sealing performance of the first sealing plate 28 and the second sealing plate 26 is improved. Preventing leakage from the syringe 4.

Example 5:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the joint 23 and the surface of the liquid outlet tubule 13 are both provided with anti-skid lines, which is convenient for a user to disassemble and assemble the liquid outlet tubule 13 and the hose 24.

Example 6:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the number of the grooves formed in the lower end of the interior of the box body 1 is 4, the balls 22 are connected in the grooves in a rolling mode, the balls 22 are connected with the first gear disc 21 in a rolling mode, and the stability of the first gear disc 21 in the rotating process is further improved.

Example 7:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the number of the vessel grooves 14 fixedly connected to the first gear plate 21 is 6, and the device can inject a plurality of culture vessels, so that the working efficiency of the device is improved.

Example 8:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: box 1 one side is connected with chamber door 2 through hinge rotation, and chamber door 2 is the transparent plate, through the setting of transparent plate, and the person of facilitating the use observes inside behavior.

Example 9:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the illuminating lamp 102 is fixedly connected to one side of the lower end of the fixing plate 5, and the illuminating lamp 102 is electrically connected with an external power supply to play a role in illumination, so that a user can observe the internal working condition conveniently.

Example 10:

referring to FIGS. 1-8, a high efficiency media injection apparatus, substantially as in example 1, further comprising: the surface of first motor 3 is equipped with soundproof cotton, reduces the noise that first motor 3 sent at the course of the work, improves user's operational environment.

It should be noted that in this patent document, the electrical components are all connected to an external master controller and a 220V commercial power point, and the master controller may be a conventional known device controlled by a computer, and it should be noted that the driving motor in the above embodiment may be, but is not limited to, a forward and reverse rotating motor with a model number of 90YR120GY 38;

according to the invention, through the arrangement of the first motor 3 and the second gear plate 101, the device realizes the characteristic of automatically injecting the culture container, the labor amount of researchers is greatly reduced, the working efficiency is improved, the backflow of the culture medium is effectively prevented through the arrangement of the first sealing plate 28 and the second sealing plate 26, the quantitative injection of the culture medium in the culture container is realized, the practicability of the device is improved, and the actual use requirements of users are further met.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The utility model provides a high-efficiency culture medium injection device, includes box (1), its characterized in that, one side fixedly connected with fixed plate (5) in box (1), fixedly connected with injection tube (4) on fixed plate (5), sliding connection has clamp plate (11) in injection tube (4), injection tube (4) upper end both sides are equipped with air vent (401), the liquid outlet of injection tube (4) is connected with out liquid tubule (13), the inlet of injection tube (4) is connected with hose (24), the lower extreme rotates through the pivot in box (1) and is connected with first toothed disc (21), be equipped with a plurality of ware grooves (14) on first toothed disc (21), go out liquid tubule (13) and ware groove (14) position phase-match, the one end fixedly connected with first motor (3) of fixed plate (5) is kept away from to the inside upper end of box (1), the box body is characterized in that a first fixing rod (12) is fixedly connected to one side of the box body (1) close to a first motor (3), a first rotating rod (6) is fixedly connected to the output end of the first motor (3), one end, far away from the first motor (3), of the first rotating rod (6) penetrates through the first fixing rod (12), a crank (8) is fixedly connected to the first rotating rod (6), one end, far away from the first rotating rod (6), of the crank (8) is rotatably connected to a connecting rod (9), one end, far away from the crank (8), of the connecting rod (9) of the connecting rod (10) is fixedly connected to a pressing plate (11), a fixing block (15) is fixedly connected to the lower end of one side of the fixing plate (5), a second rotating rod (16) is rotatably connected to the fixing block (15), and a driven wheel is fixedly connected to the second rotating rod (16), the first rotating rod (6) is fixedly connected with a driving wheel (7) matched with the driven wheel in position, the driving wheel (7) is connected with the driven wheel through a belt, one end of the second rotating rod (16) far away from the fixed block (15) is fixedly connected with a first bevel gear (17), a second fixed rod (20) is fixedly connected at the lower end of the box body (1) close to the first motor (3), a third rotating rod (19) is rotatably connected on the second fixing rod (20), the lower end of the third rotating rod (19) is rotatably connected with the bottom of the box body (1) through a rotating shaft, a second bevel gear (18) is fixedly connected at the upper end of the third rotating rod (19), the first bevel gear (17) and the second bevel gear (18) are meshed, and the lower end of the third rotating rod (19) is fixedly connected with a second gear wheel disc (101) meshed with the first gear wheel disc (21).

2. The efficient culture medium injection device according to claim 1, wherein the liquid outlet tubule (13) is connected with the injection tube (4) in a rotating way through a thread seal, two sides of the inner wall of the liquid outlet tubule (13) are fixedly connected with supporting plates (27), the supporting plates (27) are fixedly connected with first sleeves (29), the first sleeve (29) is connected with first movable columns (30) in a sliding manner, the liquid inlet of the liquid outlet tubule (13) is provided with first sealing plates (28), one ends of the two first movable columns (30) far away from the first sleeve (29) are respectively and fixedly connected with the two sides of the first sealing plates (28), the first movable column (30) is sleeved with a first return spring (31), and two ends of the first return spring (31) are fixedly connected with the first sealing plate (28) and the first sleeve (29) respectively.

3. An efficient media injector as in claim 2, characterized by that, the syringe (4) and the flexible tube (24) are connected by the screw thread of the adapter (23) in a sealing way, both sides of the liquid inlet of the injection cylinder (4) are fixedly connected with a bearing plate (25), the bearing plate (25) is fixedly connected with a second sleeve (32), the second sleeve (32) is connected with second movable columns (33) in a sliding manner, a liquid inlet of the injection cylinder (4) is provided with second sealing plates (26), one ends of the two second movable columns (33) far away from the second sleeve (32) are respectively and fixedly connected with two sides of the second sealing plates (26), and a second return spring (34) is sleeved on the second moving column (33), and two ends of the second return spring (34) are respectively fixedly connected with a second sleeve (32) and a second sealing plate (26).

4. A high efficiency media injector as in claim 3, wherein the first and second seal plates (28, 26) are each provided with a rubber cushion.

5. A high efficiency medium injector as claimed in claim 3, wherein the surface of the joint (23) and the surface of the outlet tubule (13) are provided with anti-slip lines.

6. The efficient culture medium injection device as claimed in claim 1, wherein the number of the grooves formed at the lower end of the interior of the box body (1) is 3-4, the balls (22) are connected in the grooves in a rolling manner, and the balls (22) are connected with the first gear plate (21) in a rolling manner.

7. The efficient culture medium injection device according to claim 1, wherein the number of the vessel grooves (14) fixedly connected to the first gear plate (21) is 4-6.

8. The efficient culture medium injection device as claimed in claim 1, wherein one side of the box body (1) is rotatably connected with a box door (2) through a hinge, and the box door (2) is a transparent plate.

9. An efficient culture medium injection device according to claim 1, wherein one side of the lower end of the fixing plate (5) is fixedly connected with an illuminating lamp (102), and the illuminating lamp (102) is electrically connected with an external power supply.

10. An efficient medium injection device according to claim 8, characterized in that the surface of the first motor (3) is provided with soundproof cotton.