CN111848567A - Method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn - Google Patents
Method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn Download PDFInfo
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- CN111848567A CN111848567A CN202010681730.8A CN202010681730A CN111848567A CN 111848567 A CN111848567 A CN 111848567A CN 202010681730 A CN202010681730 A CN 202010681730A CN 111848567 A CN111848567 A CN 111848567A
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- polyphenol
- purple corn
- procyanidine
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- nanofiltration
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- 240000008042 Zea mays Species 0.000 title claims abstract description 33
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 33
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 33
- 235000005822 corn Nutrition 0.000 title claims abstract description 33
- 229930002877 anthocyanin Natural products 0.000 title claims abstract description 30
- 235000010208 anthocyanin Nutrition 0.000 title claims abstract description 30
- 239000004410 anthocyanin Substances 0.000 title claims abstract description 30
- 150000004636 anthocyanins Chemical class 0.000 title claims abstract description 30
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 27
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000012528 membrane Substances 0.000 claims abstract description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000047 product Substances 0.000 claims abstract description 24
- 238000001728 nano-filtration Methods 0.000 claims abstract description 23
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 21
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 21
- 239000007789 gas Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 229910001873 dinitrogen Inorganic materials 0.000 claims abstract description 11
- 239000012535 impurity Substances 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000003651 drinking water Substances 0.000 claims abstract description 9
- 235000020188 drinking water Nutrition 0.000 claims abstract description 9
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 9
- 238000000576 coating method Methods 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000011248 coating agent Substances 0.000 claims abstract description 5
- 238000007605 air drying Methods 0.000 claims abstract description 4
- 239000008187 granular material Substances 0.000 claims abstract description 4
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 claims description 9
- 229920002414 procyanidin Polymers 0.000 claims description 9
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 claims description 8
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 claims description 8
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 claims description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 229930014669 anthocyanidin Natural products 0.000 claims description 6
- 235000008758 anthocyanidins Nutrition 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 4
- 150000001453 anthocyanidins Chemical class 0.000 claims 4
- 239000000243 solution Substances 0.000 description 21
- 235000013305 food Nutrition 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000003078 antioxidant effect Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
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- 230000002829 reductive effect Effects 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229920002770 condensed tannin Polymers 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- VEVZSMAEJFVWIL-UHFFFAOYSA-O cyanidin cation Chemical compound [O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC=C(O)C(O)=C1 VEVZSMAEJFVWIL-UHFFFAOYSA-O 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- NWKFECICNXDNOQ-UHFFFAOYSA-N flavylium Chemical compound C1=CC=CC=C1C1=CC=C(C=CC=C2)C2=[O+]1 NWKFECICNXDNOQ-UHFFFAOYSA-N 0.000 description 2
- 239000011261 inert gas Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- KZMACGJDUUWFCH-UHFFFAOYSA-O malvidin Chemical compound COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O)=C1 KZMACGJDUUWFCH-UHFFFAOYSA-O 0.000 description 2
- 230000004792 oxidative damage Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- GCPYCNBGGPHOBD-UHFFFAOYSA-N Delphinidin Natural products OC1=Cc2c(O)cc(O)cc2OC1=C3C=C(O)C(=O)C(=C3)O GCPYCNBGGPHOBD-UHFFFAOYSA-N 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 240000001549 Ipomoea eriocarpa Species 0.000 description 1
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 240000000851 Vaccinium corymbosum Species 0.000 description 1
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940093797 bioflavonoids Drugs 0.000 description 1
- 235000021014 blueberries Nutrition 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 201000011529 cardiovascular cancer Diseases 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 235000007336 cyanidin Nutrition 0.000 description 1
- 235000007242 delphinidin Nutrition 0.000 description 1
- JKHRCGUTYDNCLE-UHFFFAOYSA-O delphinidin Chemical compound [O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC(O)=C(O)C(O)=C1 JKHRCGUTYDNCLE-UHFFFAOYSA-O 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 125000004395 glucoside group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 235000009584 malvidin Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- HKUHOPQRJKPJCJ-UHFFFAOYSA-N pelargonidin Natural products OC1=Cc2c(O)cc(O)cc2OC1c1ccc(O)cc1 HKUHOPQRJKPJCJ-UHFFFAOYSA-N 0.000 description 1
- 235000006251 pelargonidin Nutrition 0.000 description 1
- XVFMGWDSJLBXDZ-UHFFFAOYSA-O pelargonidin Chemical compound C1=CC(O)=CC=C1C(C(=C1)O)=[O+]C2=C1C(O)=CC(O)=C2 XVFMGWDSJLBXDZ-UHFFFAOYSA-O 0.000 description 1
- 229930015721 peonidin Natural products 0.000 description 1
- 235000006404 peonidin Nutrition 0.000 description 1
- XFDQJKDGGOEYPI-UHFFFAOYSA-O peonidin Chemical compound C1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O)=C1 XFDQJKDGGOEYPI-UHFFFAOYSA-O 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000009822 protein phosphorylation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 239000013557 residual solvent Substances 0.000 description 1
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/08—Vasodilators for multiple indications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Toxicology (AREA)
- Alternative & Traditional Medicine (AREA)
- Vascular Medicine (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Biochemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn, which comprises the following steps: s1: crushing raw materials: coating purple corn, air drying purple corn core, and crushing into coarse granules by a crusher for later use; s2: extraction: adding drinking water into the crushed raw materials obtained in the step S1, introducing carbon dioxide gas and nitrogen gas to enable the pressure in the reaction kettle to reach 1-2MPa, maintaining the pressure for 4-6 hours, and slowly releasing the pressure to obtain the product; s3: and (3) filtering: filtering the extracting solution obtained in the step S2 by a disc centrifuge and a tubular centrifuge respectively, filtering the filtrate by an ultrafiltration membrane to remove impurities, concentrating the clear solution by a nanofiltration membrane to remove small molecular impurities and redundant water, and reserving the nanofiltration concentrated solution for later use; s4: and (3) drying: and (S3) carrying out nanofiltration on the concentrated solution, and carrying out freeze drying to prepare a mixed product containing anthocyanin, procyanidine and polyphenol. The method has the advantages of good product quality, high yield and low cost.
Description
Technical Field
The invention belongs to the technical field of extraction of plant active ingredients, and particularly relates to a method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn.
Background
Anthocyanins (also called anthocyanidins) are water-soluble natural pigments widely existing in plants in nature, and are colored aglycones obtained by hydrolyzing anthocyanins (anthocyanins). The main color-producing substances in fruits, vegetables and flowers are mostly related to the plants. Under the condition of different pH values of plant cell vacuoles, the anthocyanin enables the petals to present colorful colors. More than 20 anthocyanidins are known, and 6 important foods are pelargonidin, cyanidin, delphinidin, peonidin, morning glory pigment and malvidin. The natural anthocyanin exists in a glucoside form, is called anthocyanin, and rarely exists in a free anthocyanin. Anthocyanins are mainly used for coloring food, and can also be used in dye, medicine, and cosmetic.
Procyanidins, abbreviated as PC, is a mixture of bioflavonoids with a specific molecular structure, oligomers of which: oligomeric Proanthocyanidin (OPC) is a natural antioxidant which is internationally recognized at present and is effective in removing free radicals in human bodies. Generally a reddish brown powder, slightly smelling, astringent, soluble in water and most organic solvents. Researches show that the procyanidine extracted from the blueberry leaves can prevent the hepatitis C virus from replicating, is a bioflavonoid with a special molecular structure, and is an effective natural antioxidant capable of removing free radicals in a human body. The lipid antioxidant has strong functions of resisting oxidation and eliminating free radicals, can effectively eliminate superoxide anion free radicals and hydroxyl free radicals, also participates in metabolism of phosphoric acid and arachidonic acid and protein phosphorylation, and protects lipid from peroxidation damage; is a powerful metal chelating agent which can chelate metal ions and form inert compounds in vivo; protect and stabilize vitamin C, and is helpful for the absorption and utilization of vitamin C. The product can be used in cosmetics, and has effects of preventing sunburn, whitening skin, preventing wrinkle, resisting aging, keeping moisture, and resisting radiation.
Oxidative damage is a significant cause of many chronic diseases, such as cardiovascular diseases, cancer and aging. The antioxidant function of polyphenol can prevent these chronic diseases. In addition to the antioxidant effect, the polyphenol intake while eating high-fat food can reduce the threat of the high-fat food to the health of human bodies, which is the first discovery that the compound can effectively prevent the derivative generated by the high-fat food in the human bodies from generating adverse effect on the human bodies.
Purple corn is a unique corn variety of Peru, and corn cobs and corn kernels are purple and have extremely high phenolic compounds and anthocyanin. The purple corn contains eighteen amino acids, 21 trace elements, multiple vitamins and natural pigments which are necessary for human bodies, particularly contains anticancer element selenium, and intelligence-improving elements such as zinc, iron, calcium and the like, is excellent in taste, soft and tender, thin and smooth in skin and slightly sticky, and has special faint scent through detection of the Chinese agricultural grain quality supervision and test center, so that the food processed by the purple corn is superior natural and delicious nutritional health-care food. Meanwhile, researches find that the pigment of the purple corn has the effect of inhibiting the occurrence of cancers. In view of the effects of purple corn, the application provides a method for simultaneously extracting anthocyanin, procyanidine and polyphenol from purple corn, so as to achieve the aim of comprehensive utilization.
Disclosure of Invention
The invention aims to solve the problems and provides a method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn, and the method has the advantages of good product quality, high yield and low cost.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn comprises the following steps:
s1: crushing raw materials: coating purple corn, air drying purple corn core, and crushing into coarse granules by a crusher for later use;
s2: extraction: adding drinking water into the crushed raw materials obtained in the step S1, introducing carbon dioxide gas and nitrogen gas to enable the pressure in the reaction kettle to reach 1-2MPa, maintaining the pressure for 4-6 hours, and slowly releasing the pressure to obtain the product;
s3: and (3) filtering: filtering the extracting solution obtained in the step S2 by a disc centrifuge and a tubular centrifuge respectively, filtering the filtrate by an ultrafiltration membrane to remove impurities, concentrating the clear solution by a nanofiltration membrane to remove small molecular impurities and redundant water, and reserving the nanofiltration concentrated solution for later use;
s4: and (3) drying: and (S3) carrying out nanofiltration on the concentrated solution, and carrying out freeze drying to prepare a mixed product containing anthocyanin, procyanidine and polyphenol.
Preferably, the amount of the drinking water added in the step S2 is 5 to 10 times the amount of the crushed raw material.
Preferably, the proportion of the carbon dioxide and the nitrogen introduced in the step S2 is proportioned according to actual needs; wherein the carbon dioxide and nitrogen are combined, and can also comprise the combination of carbon dioxide and other inert gases; the ratio of 3:7 to 7:7 is preferred in the present embodiment.
Preferably, the membrane pore size of the ultrafiltration membrane in the step S3 is 1000 daltons.
Preferably, the membrane pore size of the nanofiltration membrane in the step S3 is 300 daltons.
Compared with the prior art, the invention has the beneficial effects that:
1) the traditional solvent extraction with methanol, ethanol and the like is changed, water is used as the solvent in the whole process, the problem of residual solvents such as methanol, ethanol, chloroform and the like in the product is avoided, the environmental pollution is avoided, and the cost of the product is greatly reduced;
2) carbon dioxide and nitrogen are used for pressurization, and simultaneously, part of carbon dioxide is dissolved in water to generate carbonic acid, so that the aqueous solution keeps acidic, the anthocyanin, the procyanidine and the polyphenol product are stably protected, and the use of inorganic acids such as sulfuric acid, hydrochloric acid and the like and other organic acids in the traditional method is avoided;
3) the extraction rate is improved by using gas for pressurized extraction;
4) carbon dioxide and nitrogen are used for pressurization, so that oxidative damage of oxygen and the like to anthocyanin, procyanidine and polyphenol products is avoided, and the content and yield of the products are improved;
5) Normal temperature and low temperature are used in the whole process, heating of each link in the traditional method is avoided, energy consumption is reduced, and product damage caused by temperature is reduced.
The technical scheme of the invention extracts three effective components from the coating and the core simultaneously, thus achieving the purpose of comprehensive utilization, and the product prepared by the method has good quality, high yield, low cost and wide market prospect.
Detailed Description
In order to make the technical solutions of the present invention better understood and implemented by those skilled in the art, the present invention is further described with reference to the following specific examples, which are provided for illustration only and are not intended to limit the present invention.
The technical scheme of the invention is to provide a method for simultaneously extracting and preparing anthocyanin, procyanidine and polyphenol from waste corn coatings and corncobs in the purple corn planting process, and the method is simple and easy to implement, low in cost and good in product quality.
The method is realized by the following steps:
s1: crushing raw materials: coating purple corn, air drying purple corn core, and crushing into coarse granules by a crusher for later use;
s2: extraction: adding 5-10 times of drinking water into the crushed raw materials, and then introducing carbon dioxide gas and nitrogen gas, wherein the ratio of the carbon dioxide gas to the nitrogen gas is 3: 7-7: 7; introducing gas to make the pressure in the reaction kettle reach 1-2MPa, maintaining the pressure for 4-6 hours, and then slowly releasing the pressure;
S3: and (3) filtering: filtering the above extractive solutions with disc centrifuge and tubular centrifuge respectively, filtering the filtrate with ultrafiltration membrane with membrane aperture of 1000 daltons to remove impurities such as protein and saccharide, concentrating the clear solution with nanofiltration membrane with membrane aperture of 300 daltons to remove small molecular impurities and excessive water, and collecting the nanofiltration concentrated solution;
s4: and (3) drying: and (4) carrying out nanofiltration on the concentrated solution in the previous step, and carrying out freeze drying to prepare a mixed product containing anthocyanin, procyanidine and polyphenol.
Example 1:
1. crushing raw materials: crushing 500kg of dried purple corn coatings into coarse particles by a crusher for later use;
2. extraction: adding 10 times of drinking water into the crushed raw materials, and then introducing carbon dioxide gas and nitrogen gas, wherein the ratio of the carbon dioxide gas to the nitrogen gas is 3: 7; introducing gas to enable the pressure in the reaction kettle to reach 1MPa, maintaining the pressure for 4 hours, and then slowly releasing the pressure to obtain the product;
3. and (3) filtering: filtering the above extractive solutions with disc centrifuge and tubular centrifuge respectively, filtering the filtrate with ultrafiltration membrane with membrane aperture of 1000 Dalton, concentrating the clear solution with nanofiltration membrane with membrane aperture of 300 Dalton, removing small molecular impurities and excessive water, and collecting the nanofiltration concentrated solution;
4. And (3) drying: and (3) carrying out nanofiltration on the concentrated solution in the previous step, and freeze-drying to obtain 5.3kg of a mixed product containing anthocyanin, procyanidin and polyphenol, wherein the anthocyanin content is 29.5%, the procyanidin content is 68%, and the polyphenol content is 75%.
Example 2:
1. crushing raw materials: crushing 500kg of dried purple corn cores into coarse particles by a crusher for later use;
2. extraction: adding 8 times of drinking water into the crushed raw materials, and then introducing carbon dioxide gas and nitrogen gas, wherein the ratio of the carbon dioxide gas to the nitrogen gas is 5: 7; introducing gas to enable the pressure in the reaction kettle to reach 2MPa, maintaining the pressure for 5 hours, and then slowly releasing the pressure to obtain the product;
3. and (3) filtering: filtering the above extractive solutions with disc centrifuge and tubular centrifuge respectively, filtering the filtrate with ultrafiltration membrane with membrane aperture of 1000 Dalton, concentrating the clear solution with nanofiltration membrane with membrane aperture of 300 Dalton, removing small molecular impurities and excessive water, and collecting the nanofiltration concentrated solution;
4. and (3) drying: and (3) carrying out nanofiltration on the concentrated solution in the previous step, and freeze-drying to obtain 6.8kg of a mixed product containing anthocyanin, procyanidin and polyphenol, wherein the anthocyanin content is 31.5%, the procyanidin content is 65%, and the polyphenol content is 73%.
Example 3:
1. Crushing raw materials: crushing 500kg of dried purple corn coatings into coarse particles by a crusher for later use;
2. extraction: adding 5 times of drinking water into the crushed raw materials, and then introducing carbon dioxide gas and nitrogen gas, wherein the ratio of the carbon dioxide gas to the nitrogen gas is 7: 7; introducing gas to enable the pressure in the reaction kettle to reach 1.5MPa, maintaining the pressure for 6 hours, and then slowly releasing the pressure to obtain the product;
3. and (3) filtering: filtering the above extractive solutions with disc centrifuge and tubular centrifuge respectively, filtering the filtrate with ultrafiltration membrane with membrane aperture of 1000 Dalton, concentrating the clear solution with nanofiltration membrane with membrane aperture of 300 Dalton, removing small molecular impurities and excessive water, and collecting the nanofiltration concentrated solution;
4. and (3) drying: and (3) carrying out nanofiltration on the concentrated solution in the previous step, and freeze-drying to obtain 5.6kg of a mixed product containing anthocyanin, procyanidin and polyphenol, wherein the anthocyanin content is 28.7%, the procyanidin content is 71%, and the polyphenol content is 74%.
The proportion of carbon dioxide and nitrogen is proportioned according to actual needs; wherein the carbon dioxide and nitrogen are combined, and can also comprise the combination of carbon dioxide and other inert gases; the ratio of 3:7 to 7:7 is preferred in the present embodiment.
The details of the present invention not described in detail are prior art.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. A method for simultaneously preparing anthocyanin, procyanidine and polyphenol from purple corn is characterized by comprising the following steps:
s1: crushing raw materials: coating purple corn, air drying purple corn core, and crushing into coarse granules by a crusher for later use;
s2: extraction: adding drinking water into the crushed raw materials obtained in the step S1, introducing carbon dioxide gas and nitrogen gas to enable the pressure in the reaction kettle to reach 1-2MPa, maintaining the pressure for 4-6 hours, and slowly releasing the pressure to obtain the product;
s3: and (3) filtering: filtering the extracting solution obtained in the step S2 by a disc centrifuge and a tubular centrifuge respectively, filtering the filtrate by an ultrafiltration membrane to remove impurities, concentrating the clear solution by a nanofiltration membrane to remove small molecular impurities and redundant water, and reserving the nanofiltration concentrated solution for later use;
s4: and (3) drying: and (S3) carrying out nanofiltration on the concentrated solution, and carrying out freeze drying to prepare a mixed product containing anthocyanin, procyanidine and polyphenol.
2. The method for simultaneously preparing anthocyanidin, procyanidine and polyphenol from purple corn as claimed in claim 1, wherein the amount of drinking water added in step S2 is 5-10 times of the amount of the crushed raw material.
3. The method for simultaneously preparing anthocyanidin, procyanidine and polyphenol from purple corn as claimed in claim 1, wherein the ratio of carbon dioxide and nitrogen introduced in step S2 is proportioned according to actual needs.
4. The method for simultaneously preparing anthocyanidin, procyanidin and polyphenol from purple corn as claimed in claim 1, wherein the membrane pore size of the ultrafiltration membrane in the step S3 is 1000 Dalton.
5. The method for simultaneously preparing anthocyanidin, procyanidin and polyphenol from purple corn as claimed in claim 1, wherein the membrane pore size of the nanofiltration membrane in step S3 is 300 Dalton.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113826736A (en) * | 2021-08-23 | 2021-12-24 | 朱海英 | Purple corn substituted tea and preparation method thereof |
| CN117530445A (en) * | 2023-12-22 | 2024-02-09 | 广州泽力医药科技有限公司 | Purple corn compound beverage and preparation method thereof |
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| CN106008443A (en) * | 2016-07-22 | 2016-10-12 | 河南科技大学 | Extraction method of purple corn anthocyanin |
| CN107033118A (en) * | 2017-04-19 | 2017-08-11 | 北海市韩能生物科技有限公司 | The extracting method of OPC |
| CN107552536A (en) * | 2017-09-05 | 2018-01-09 | 贵州慧静生物科技有限公司 | Grape wine waste residue integrated extraction technique |
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| CN105601686A (en) * | 2016-02-02 | 2016-05-25 | 长沙蓝威生物制品有限公司 | Method for extracting cyanidin from roselle |
| CN106008443A (en) * | 2016-07-22 | 2016-10-12 | 河南科技大学 | Extraction method of purple corn anthocyanin |
| CN107033118A (en) * | 2017-04-19 | 2017-08-11 | 北海市韩能生物科技有限公司 | The extracting method of OPC |
| CN107552536A (en) * | 2017-09-05 | 2018-01-09 | 贵州慧静生物科技有限公司 | Grape wine waste residue integrated extraction technique |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113826736A (en) * | 2021-08-23 | 2021-12-24 | 朱海英 | Purple corn substituted tea and preparation method thereof |
| CN117530445A (en) * | 2023-12-22 | 2024-02-09 | 广州泽力医药科技有限公司 | Purple corn compound beverage and preparation method thereof |
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